Tokushima University / Educator and Researcher Directory --- Wada -Mihara, Chie

Personal Web Page

https://researchmap.jp/mihachie (researchmap)

Field of Study

○	Dentistry

Subject of Study

○

Book / Paper

Academic Paper (Judged Full Paper):

1.	Susumu Abe, Yoshizo Matsuka, Ayu Murakami, Omar Maningo Rodis, Kenji Oka, Toshinori Okawa, Chie Wada -Mihara, Chihiro Shinohara and Fumiaki Kawano : Job shadowing for early undergraduate dental students: Validation of educational effectiveness using structural equation modeling, European Journal of Dental Education, Vol.27, No.4, 898-907, 2023. (Summary) This study aimed to evaluate and validate the educational effectiveness of a job shadowing class as a dental teaching programme for preclinical dental students through a questionnaire that assessed related student perceptions. A total of 124 preclinical (second year) dental students at Tokushima University participated in job shadowing placements from 2017 to 2019. Each student observed dental practice or surgery for 1 day in the university hospital. After shadowing, the students were requested to fill out a nine-item questionnaire based on a five-point scale. The first analysis was performed to calculate the correlation for each item. Furthermore, regression analysis for the categorical data was performed to show the relationship between eight questions (Items 1-8) and one question (Item 9: comprehensive evaluation). Next, factor analysis was performed to determine students' perceptions regarding the shadowing class. Ninety-eight students (response rate: 79%) completed the questionnaire. There was a strong relationship amongst Items 1, 8 and 9 ("interested in dentistry as a job," "learning motivation" and "comprehensive evaluation" respectively) based on the results of the correlation and regression analysis. The factor analysis led to three student perceptions as latent factors, namely, "Interesting," "Self-awareness" and "Future Goal," which had a relatively close inter-factor correlation (0.465-0.772). The finding suggests that job shadowing may increase a students' interest in a dentistry career at an early stage of their education and learning motivation, along with generating self-awareness. This programme may present possible educational effectiveness in dental education. (Link to Publication Site) ● Publication site (DOI): 10.1111/eje.12880 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36484175 ● Search Scopus @ Elsevier (PMID): 36484175 ● Search Scopus @ Elsevier (DOI): 10.1111/eje.12880 (DOI: 10.1111/eje.12880, PubMed: 36484175)
2.	Toshinori Okawa, Susumu Abe, Keiichiro Watanabe, Kouhei Kamoi, Kenji Oka, Chie Wada -Mihara, Akira Imakiire, Taeko Masuda, Nanoha Matsumoto, Chihiro Shinohara and Fumiaki Kawano : Possibility and Future of Intraoral Scanner Application to Change the Method of Taking Impressions, Journal of Oral Health and Biosciences, Vol.35, No.2, 33-38, 2023. (Keyword) Intraoral Scanner / Precise Impression / Digital Dentistry (Tokushima University Institutional Repository) ● Metadata: 118166 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.35.2_33 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390014093884084608 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.35.2_33 (Tokushima University Institutional Repository: 118166, DOI: 10.20738/johb.35.2_33, CiNii: 1390014093884084608)
3.	Koji Naruishi, Chie Wada -Mihara, Keiji Oishi and Toshihiko Nagata : Dental students' awareness after clinical training between dental treatment and systemic health: A questionnaire-based survey, Frontiers in Dental Medicine, 2022. (Link to Publication Site) ● Publication site (DOI): 10.3389/fdmed.2021.740441 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3389/fdmed.2021.740441 (DOI: 10.3389/fdmed.2021.740441)
4.	Chie Wada -Mihara, Hiroyuki Seto, Hirofumi Ohba, Kaku Tokunaga, Jun-ichi Kido, Toshihiko Nagata and Koji Naruishi : Local administration of calcitonin inhibits alveolar bone loss in an experimental periodontitis in rats., Biomedicine & Pharmacotherapy, Vol.97, No.1, 765-770, 2018. (Summary) Calcitonin (CTN), a calcium regulatory hormone, promotes calcium diuresis from the kidney and suppresses bone resorption. The objective of this study was to evaluate whether the topical and intermittent application of CTN inhibits alveolar bone resorption using ligature-induced experimental periodontitis in rats. Experimental periodontitis was induced by placing a nylon ligature around maxillary molars of 8-week-old male Wistar rats for 20 days. Thirty-two rats were divided into four groups: basal sham control group, periodontitis group, periodontitis plus 0.2 U CTN (low dose), and periodontitis plus 1.0 U CTN (high dose) group. To investigate the effects of CTN on alveolar bone resorption, CTN was topically injected into the palatal gingivae every 2 days after ligature removal (day 0). Micro-computed tomography (CT) analysis was performed for linear parameter assessment of alveolar bone on day 5 and day 14. Periodontal tissues were examined histo-pathologically to assess the differences among the study groups. Micro-CT images showed that alveolar bone resorption was induced statistically around the molar of ligatured rats on day 5 and day 14. The amount of bone resorption was more severe on day 14 than that on day 5. On day 5, only high-dose CTN treatment significantly suppressed bone resorption. In addition, both doses of CTN significantly suppressed bone resorption on day 14. Histological examination clarified that there were fewer TRAP-positive cells in the CTN treatment groups than in the periodontitis group on day 5. Local administration of CTN decreased alveolar bone resorption by regulating osteoclast activation in rats with periodontitis. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.biopha.2017.10.165 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29107933 ● Search Scopus @ Elsevier (PMID): 29107933 ● Search Scopus @ Elsevier (DOI): 10.1016/j.biopha.2017.10.165 (DOI: 10.1016/j.biopha.2017.10.165, PubMed: 29107933)
5.	Chie Wada -Mihara, Koji Naruishi, Mika Bandou, Yasufumi Nishikawa, Lew Jung-Hwan, Eijiro Sakamoto, Takahisa Ikuta, Kaoru Kono, Yukari Kajiura, Mari Hashimoto, Yukiko Nakajima, Yuji Inagaki, Masami Ninomiya, Jun-ichi Kido and Toshihiko Nagata : Post clinical clerkship-OSCEを見据えた歯周病科での模擬OSCEの概要, Journal of Oral Health and Biosciences, Vol.29, No.2, 49-53, 2017. (Tokushima University Institutional Repository) ● Metadata: 111059 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.29.2_49 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.20738/johb.29.2_49 (Tokushima University Institutional Repository: 111059, DOI: 10.20738/johb.29.2_49)
6.	Eijiro Sakamoto, Chie Wada -Mihara, Takahisa Ikuta, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : Inhibitory effects of advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide on the expression of osteoblastic markers of rat bone marrow cells in culture, Journal of Periodontal Research, Vol.51, No.3, 313-320, 2016. (Summary) Diabetes is a major risk factor for periodontitis and there is a close relationship between the degree of hyperglycemia and the severity of periodontitis. Advanced glycation end-products (AGEs) accumulate in various tissues under diabetic conditions. AGEs in the periodontal tissues probably play a role in upregulating periodontal inflammation; however, the association of AGEs with the severity of periodontitis has not been fully clarified. Lipopolysaccharide from Porphyromonas gingivalis (P-LPS) is a potent pathogenic factor in periodontitis. Although the independent effect of AGE or P-LPS on osteoblastic cells has been reported in vitro, the effect of adding both has not been clearly elucidated. In this study, to explore factors aggravating diabetic periodontitis, we investigated the effects of AGE and P-LPS on the expression of osteoblastic markers and the expression of inflammation-related markers in vitro. Rat bone marrow cells were cultured, and alkaline phosphatase activity and bone nodule formation were evaluated as osteoblastic markers. Reverse transcription-polymerase chain reaction was performed to determine the mRNA expression of molecules associated with bone and inflammation. Protein levels of osteocalcin and interleukin-1β (IL-1β) were measured using enzyme-linked immunosorbent assay. AGEs and P-LPS independently reduced alkaline phosphatase activity and bone nodule formation. The addition of both AGE and P-LPS (AGE+P-LPS) further decreased these markers. Reverse transcription-polymerase chain reaction analysis revealed that AGE+P-LPS markedly decreased the mRNA expression of osteoblast-related molecules such as type 1 collagen, osteocalcin and Cbfa1, and markedly increased that of inflammation-related molecules such as IL1β and S100A8. AGE and P-LPS decreased the protein level of osteocalcin and increased that of IL-1β, and a further increase of IL-1β was detected for AGE+P-LPS. AGEs and P-LPS inhibited the expression of osteoblastic markers and increased the levels of inflammatory markers in rat bone marrow cells, suggesting that both AGE and P-LPS may be important factors associated with the aggravation of diabetic periodontitis. (Keyword) advanced glycation end products / diabetes / osteoblastic differentiation / periodontitis (Link to Publication Site) ● Publication site (DOI): 10.1111/jre.12310 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26223811 ● Summary page in Scopus @ Elsevier: 2-s2.0-84938353798 (DOI: 10.1111/jre.12310, PubMed: 26223811, Elsevier: Scopus)
7.	Chie Wada -Mihara, 徳永格, Hiroyuki Seto, Eijiro Sakamoto, Yuka Hiroshima, Jun-ichi Kido and Toshihiko Nagata : Topical injection of osteoprotegerin prevents alveolar bone loss in rat experimental periodontitis, Journal of the Japanese Association of Periodontology, Vol.54, No.2, 167-174, 2012. (Keyword) osteoprotegerin / experimental periodontitis / alveolar bone / osteoclast (Link to Publication Site) ● Publication site (DOI): 10.2329/perio.54.167 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679391110400 ● Search Scopus @ Elsevier (DOI): 10.2329/perio.54.167 (DOI: 10.2329/perio.54.167, CiNii: 1390282679391110400)
8.	Yuka Hiroshima, Mika Bandou, Yuji Inagaki, Chie Wada -Mihara, Masatoshi Kataoka, Hiromi Murata, Yasuo Shinohara, Toshihiko Nagata and Jun-ichi Kido : Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils, Journal of Periodontal Research, Vol.47, No.5, 554-562, 2012. (Summary) Resistin is an adipocytokine that induces insulin resistance and is predominantly expressed in adipocytes and peripheral blood mononuclear cells. Resistin expression increases in inflammatory diseases as well as diabetes mellitus, and is upregulated by bacterial pathogens and proinflammatory cytokines. The aim of this study was to identify resistin in human gingival crevicular fluid, to compare the resistin levels in gingival crevicular fluid between subjects with and without periodontitis and diabetes mellitus and to investigate the regulation of resistin release from human neutrophils by Porphyromonas gingivalis lipopolysaccharide (P-LPS). Gingival crevicular fluid samples were collected from patients with chronic periodontitis (n = 24), patients with diabetes mellitus-related periodontitis (n = 18) and healthy subjects (n = 21). Resistin in gingival crevicular fluid was determined using western blot analysis and an ELISA kit. The glycated hemoglobin (HbA(1c)) value was obtained from patients with diabetes mellitus-related periodontitis by a medical interview. Human neutrophils were cultured with P-LPS (0-1000 ng/mL), or incubated with inhibitors of actin or microtubule polymerization in the absence or presence of P-LPS. The medium and cellular fractions were used for determination of resistin by ELISA. The resistin level in gingival crevicular fluid from patients with periodontitis or diabetes mellitus-related periodontitis was significantly higher than that of healthy subjects. The resistin level in gingival crevicular fluid was correlated with gingival index score, but not blood HbA(1c) value. The P-LPS increased resistin release from human neutrophils, and its induction was decreased by actin polymerization inhibitors. We show, for the first time, the presence of resistin in gingival crevicular fluid. A high resistin level in gingival crevicular fluid samples from periodontitis patients may to some extent be related to P-LPS-induced resistin release from neutrophils. (Keyword) Actin Capping Proteins / Adult / Aged / Cell Culture Techniques / Chronic Periodontitis / Colchicine / Cytochalasin B / Cytochalasin D / Diabetes Complications / Female / Gingival Crevicular Fluid / Hemoglobin A, Glycosylated / Humans / Lipopolysaccharides / Male / Middle Aged / Neutrophils / Nocodazole / Nucleic Acid Synthesis Inhibitors / Periodontal Index / Periodontal Pocket / Periodontitis / Porphyromonas gingivalis / Resistin / Tubulin Modulators (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-0765.2011.01466.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22309231 ● Summary page in Scopus @ Elsevier: 2-s2.0-84865553635 (DOI: 10.1111/j.1600-0765.2011.01466.x, PubMed: 22309231, Elsevier: Scopus)
9.	Kaku Tokunaga, Hiroyuki Seto, Hirofumi Ohba, Chie Wada -Mihara, Hideki Hama, Masumi Horibe, Satoshi Yoneda and Toshihiko Nagata : Topical and intermittent application of parathyroid hormone recovers alveolar bone loss in rat experimental periodontitis., Journal of Periodontal Research, Vol.46, No.6, 655-662, 2011. (Summary) Periodontitis is characterized by periodontal tissue inflammation and alveolar bone loss. The intermittent administration of parathyroid hormone (PTH), a major regulator of bone remodeling, has been demonstrated to stimulate osteoblastic activity. Although the systemic administration of PTH has been reported to protect against periodontitis-associated bone loss, the effect of the topical administration of PTH is unclear. In this study, the effect of intermittent administration of PTH on osteoblastic differentiation was examined in cultured calvaria cells and then the effect of topical and intermittent administration of PTH was determined by measuring the recovery of alveolar bone loss after inducing experimental periodontitis in rats. Alkaline phosphatase activity and bone nodule formation were measured in fetal rat calvaria cells. Experimental periodontitis was induced by placing nylon ligature around rat maxillary molars for 20 d. After ligature removal (day 0), PTH was topically injected into buccal gingiva three times a week for 10 wk. Micro-computed tomography analysis and histological examination were performed on days 35 and 70. Intermittent exposure of PTH in calvaria cells increased alkaline phosphatase activity and bone nodule formation by 1.4- and 2.4-fold, respectively. Ligature procedures induced marked alveolar bone loss around the molars on day 0 and greater bone recovery was observed in the PTH-treated rats on day 70. An increase in osteoid formation on the surface of alveolar bone was detected in the PTH-treated rats. Intermittent treatment with PTH stimulated osteoblastic differentiation in fetal rat calvaria cell cultures, and topical and intermittent administration of PTH recovered alveolar bone loss in rat experimental periodontitis. (Keyword) Administration, Topical / Alkaline Phosphatase / Alveolar Bone Loss / Animals / Bone Regeneration / Cell Differentiation / Cells, Cultured / Drug Administration Schedule / Fetus / Image Processing, Computer-Assisted / Ligation / Osteoblasts / Parathyroid Hormone / Periodontitis / Rats / Rats, Inbred F344 / Rats, Wistar / Skull / X-Ray Microtomography (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-0765.2011.01386.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21722135 ● Search Scopus @ Elsevier (PMID): 21722135 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2011.01386.x (DOI: 10.1111/j.1600-0765.2011.01386.x, PubMed: 21722135)
10.	Noriko Hayashi, Jun-ichi Kido, (名) Suryono, Reiko Kido, Chie Wada -Mihara, Masatoshi Kataoka, Yasuo Shinohara and Toshihiko Nagata : Regulation of Calprotectin Expression by IL-1α and TGF-β in Human Gingival Keratinocytes, Journal of Periodontal Research, Vol.42, No.1, 1-7, 2007. (Summary) Calprotectin, a heterodimer of S100A8 and S100A9 with antimicrobial properties, is expressed in gingival keratinocytes and plays an important role in innate immunity. Because calprotectin expression is localized in the spinous cell layer of the gingival epithelium, we hypothesized that the expression of calprotectin in keratinocytes is related to the differentiation stage. The aim of the present study was to investigate the relationship between calprotectin expression and keratinocyte differentiation using some factors that regulated its differentiation. Normal human gingival keratinocytes were isolated from gingival tissues obtained at the extraction of wisdom teeth, and were cultured in serum-free keratinocyte medium supplemented with interleukin-1alpha or calcium, which promote keratinocyte differentiation, and transforming frowth factor-beta (TGF-beta) or retinoic acid, which suppress its differentiation. The expression of S100A8/A9 mRNA and the production of calprotectin in normal human gingival keratinocytes were examined by northern blotting and enzyme-linked immunosorbent assay, respectively. The expression of cytokeratin 14, involucrin and filaggrin (marker proteins of keratinocyte differentiation) was investigated by immunohistochemical staining, and the DNA-binding activity of CCAAT/enhancer binding protein alpha (C/EBPalpha), a transcription factor, was examined by electrophoretic mobility shift assay. The expression of S100A8/A9 mRNA and the production of calprotectin were increased by interleukin-1alpha and calcium, but decreased by TGF-beta. RA inhibited the expression of S100A8/A9 and keratinocyte differentiation, which were induced by interleukin-1alpha. C/EBPalpha DNA-binding activity in normal human gingival keratinocytes was enhanced by interleukin-1alpha and calcium, but suppressed by TGF-beta. The present study suggests that calprotectin expression is related to keratinocyte differentiation and that C/EBPalpha is a regulator of calprotectin expression in keratinocytes. (Keyword) calprotectin / gingival keratinocyte / differentiation / C/EBPα (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1600-0765.2005.00857.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17214633 ● Search Scopus @ Elsevier (PMID): 17214633 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2005.00857.x (DOI: 10.1111/j.1600-0765.2005.00857.x, PubMed: 17214633)
11.	Chie Wada -Mihara, Masatoshi Kataoka, Hiroyuki Seto, Noriko Hayashi, Jun-ichi Kido, Yasuo Shinohara and Toshihiko Nagata : High-turnover osteoporosis is induced by cyclosporin A in rats., Journal of Bone and Mineral Metabolism, Vol.24, No.3, 199-205, 2006. (Summary) Cyclosporin A (CsA) is used widely as an immunosuppressive agent, but it induces osteoporosis as a prominent side effect. To elucidate the mechanisms involved in CsA-induced osteoporosis, the effects of CsA on bone metabolism were investigated in a rat experimental model. Fifteen-day-old rats were fed a powdered diet containing or lacking CsA for 8-30 days. Analysis was performed by micro-computed tomography (muCT) and light microscopy to examine histomorphometric changes in rat tibiae on days 8, 16, and 30. Plasma parathyroid hormone (PTH) and osteocalcin (OCN) levels were determined by enzyme-linked immunosorbent assay (ELISA) on days 8, 16, and 30. The expression of OCN, osteopontin (OPN), and cathepsin K mRNAs in tibial bone marrow was examined by Northern blot analysis on days 8 and 16. Although no significant differences were observed in tibial length during the experimental periods, or in histomorphometric parameters on day 8, an apparent decrease in bone volume was observed in the CsA-treated group after day 16. Histologic analysis showed that the number of osteoblasts and osteoclasts on the surface of trabecular bone in the CsA-treated group had increased significantly on day 16. Plasma PTH and OCN levels in CsA-treated rats were significantly higher than those in control animals on day 8. Northern blot analysis revealed that the CsA-treated group showed an increase in the expression of OCN, OPN, and cathepsin K mRNAs on day 8 compared with the controls. These findings suggest that bone resorption in CsA-treated rats is induced by high-turnover osteoporosis and that bone remodeling activity may be activated by PTH. (Keyword) Animals / Bone Remodeling / Bone Resorption / Cyclosporine / Gingival Overgrowth / Immunosuppressive Agents / Male / Osteocalcin / Osteogenesis / Osteoporosis / Parathyroid Hormone / Rats / Rats, Inbred F344 / Tibia / Tomography, X-Ray Computed (Link to Publication Site) ● Publication site (DOI): 10.1007/s00774-005-0672-x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16622732 ● Search Scopus @ Elsevier (PMID): 16622732 ● Search Scopus @ Elsevier (DOI): 10.1007/s00774-005-0672-x (DOI: 10.1007/s00774-005-0672-x, PubMed: 16622732)
12.	Mari Ogino, Jun-ichi Kido, Mika Bando, Noriko Hayashi, Chie Wada -Mihara, Toshihiko Nagata, Fusanori Nishimura, Y. Soga, Shogo Takashiba, T. Kubota, M. Itagaki, Yasuko Shimada, H. Tai, Hiromasa Yoshie, Naoshi Yamazaki, Yasuo Shinohara and Masatoshi Kataoka : Alpha 2 integrin +807 polymorphism in drug-induced gingival overgrowth, Journal of Dental Research, Vol.84, No.12, 1183-1186, 2005. (Summary) Alpha2 integrin on fibroblasts is reported to play an important role in the induction of drug-induced gingival overgrowth, which is characterized by excessive accumulation of type I collagen in gingival connective tissue. Silent polymorphism 807 T/C within the alpha2 integrin gene is associated with high/low alpha2 integrin expression. The aim of this study was to test the hypothesis that expression of alpha2 integrin 807 T/C polymorphism correlates with drug-induced gingival overgrowth. A case-control study comparing 136 subjects taking calcium channel blockers (72 with vs. 64 without drug-induced gingival overgrowth) demonstrated that the frequency of the +807 C allele was significantly higher in the case group than in the controls (odds ratio, 3.61; 95% confidence interval, 2.14 - 6.10; P < 0.05). The present findings suggest that the alpha2 +807 C allele is one of the genetic risk factors for drug-induced gingival overgrowth. (Keyword) drug-induced gingival overgrowth / Alpha 2 integrin / SNP / collagen phagocytosis (Link to Publication Site) ● Publication site (DOI): 10.1177/154405910508401217 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16304451 ● Search Scopus @ Elsevier (PMID): 16304451 ● Search Scopus @ Elsevier (DOI): 10.1177/154405910508401217 (DOI: 10.1177/154405910508401217, PubMed: 16304451)

Academic Paper (Unrefereed Paper):

1.	Jun-ichi Kido, Mika Bandou, Yuji Inagaki, 生田貴久, Yukari Kajiura, Yukiko Nakajima, Eijiro Sakamoto, Chie Wada -Mihara, Masami Ninomiya, 西川泰史, Jung-Hwan Lew, Koji Naruishi and Toshihiko Nagata : Diagnosis of Periodontal Diseases using Biomarkers in Gingival Crevicular Fluid, Journal of Oral Health and Biosciences, Vol.29, No.1, 13-20, 2016. (Summary) Periodontal diseases cause an inflammation and degradation of periodontal tissues and missing of teeth. The incidence rate of periodontal diseases is high in middle-aged and elderly people.A reasonable diagnosis of periodontal diseases is very important to keep teeth, however, conventional examinations of periodontal diseases is not necessarily exact and objective. Gingival crevicular fluid (GCF) is an exudate secreted from periodontal tissues and contains many components including proteolytic enzymes, inflammatory cytokines, blood-associated proteins, cellular and bacterial fragments. Because some proteins in GCF are related to inflammation, tissue degradation and bone metabolism, those proteins have been studying as a diagnostic marker of periodontal diseases. GCF is noninvasively collected using a sterile paper strip and biomarkers are determined using enzyme-linked immunosorbent assay (ELISA) and enzyme activity assay. We identified calprotectin, an inflammationrelated protein, in GCF and calprotectin level in GCF from periodontitis sites was significantly higher than that of healthy control. Calprotectin level in GCF was positively correlated to gingival index and other biomarkers and decreased by periodontal treatments. Resistin is an adipocytokine and its level increases in some inflammatory diseases. Resistin level in GCF from periodontitis sites was high compared to the level of healthy control samples. Procollagen type I C-terminal peptide (PICP) is a biomarker for bone metabolism and its level was high in GCF collected from periodontitis sites. These results suggested that calprotectin, resistin and PICP are useful biomarkers for periodontal diseases. On the other hand, we showed that glycated albumin (GA), a marker of diabetes mellitus (DM), was contained in GCF and GA level in GCF from DM patients was significantly higher than that of non-DM individuals. Components in GCF may be biomarkers of systemic diseases as well as periodontal diseases and their determination will be useful diagnostic examination of some diseases. Recently, we have been studying the determining system of GCF calprotectin, including microchip ELISA, surface plasmon resonance assay and immuno-chromatography assay. When GCF biomarkers are determined using the determining systems, we will simply, exactly and objectively diagnose periodontal diseases at our dental offices. (Keyword) 歯周病診断 / バイオマーカー / 歯肉溝滲出液 (Tokushima University Institutional Repository) ● Metadata: 109875 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050020697877556480 (Tokushima University Institutional Repository: 109875, CiNii: 1050020697877556480)

Review, Commentary:

1.	Toshihiko Nagata, Masami Ninomiya, Chie Wada -Mihara, Jun-ichi Kido and Seiji Nishikawa : Etiology of drug-induced gingival overgrowth, Current Issues in Periodontics, 1-8, Oct. 2016. (Keyword) Drug-induced gingival overgrowth / epidemiology / Calcium-channel blocker

Proceeding of International Conference:

1.	E Sakamoto, Chie Wada -Mihara, T Ikuta, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : Age and LPS inhibit osteoblastic differentiation in rat bone marrow cells., international Associatuin of dental Research, Seattle,
2.	E Sakamoto, Chie Wada -Mihara, K Tokunaga, H Seto and Yuka Hiroshima : Topical application of osteoprotegerin inhibits alveolar bone loss in rat experimental periodontitis, International Joint Symposium on Oral Science, Bali, Indonesia, Dec 17-19, 2010,
3.	Yuka Hiroshima, Mika Bandou, Takahiro Hasegawa, Yuji Inagaki and Chie Wada -Mihara : Resistin release from neutrophils is induced by Porphyromonas gingivalis lipopolysaccharide., IADR (International Association of Dental Research) Barcelona, Spain, July 14-17, 2010,
4.	Jun-ichi Kido, Mika Bandou, Yuji Inagaki, Yuka Hiroshima, Hiromi Murata, Chie Wada -Mihara, 梶浦由加里, 生田貴久, 篠原宏貴, 橋本万里, Yukiko Nakajima, Yukiko Bandou, Makoto Funaki, Haruhiko Saito and Toshihiko Nagata : Diagnosis of diabetes-associated periodontitis using glycoalbumin and calprotectin in gingival crevicular fluid, 10th Asian Pacific Society of Periodontology Meeting, Nara, Sep. 2013.
5.	E Sakamoto, Chie Wada -Mihara, T Ikuta, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : AGE and LPS inhibits osteoblastic differentiation in rat bone-marrow cells, 91th General Session & Exhibition of the International Association for Dental Research, Settle, USA, May 2013.
6.	Toshihiko Nagata, Jun-ichi Kido, Mika Bandou, Yuka Hiroshima, Yuji Inagaki, Hiromi Murata, Chie Wada -Mihara, Mika Bandou and Makoto Funaki : Diagnosis of diabetes-associated periodontitis using biomarkers in gingival crevicular fluid, 91th General Session & Exhibition of the International Association for Dental Research. Seattle, USA, May 2013.
7.	Eijiro Sakamoto, Chie Wada -Mihara, Takahisa Ikuta, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : Age and LPS inhibit osteoblastic differentiation in rat bone marrow cells., international Associatuin of dental Research, Seattle, Mar. 2013.
8.	Toshihiko Nagata, Jun-ichi Kido, Mika Bandou, Yuka Hiroshima, Yuji Inagaki, Masami Ninomiya, Chie Wada -Mihara, Hiromi Murata, Yukiko Bandou and Makoto Funaki : Diagnosis of diabetes-associated periodontitis by measuring biomarkers in gingival crevicular fluid, The 9th International Diabetes Federation Western Pacific Region Congress/ The 4th AASD Scientific Meeting, Nov. 2012.

Proceeding of Domestic Conference:

1.	Toshinori Okawa, Susumu Abe, Chie Wada -Mihara, Hinayo Umeda, Ayumi Matsura, 山本彩瑛 and Kenji Oka : Research of The Measurement Precision in Intraoral Scanner, 第16回日本総合歯科学会総会・学術大会, 67, Oct. 2023.
2.	Ayumi Matsura, Hinayo Umeda, Susumu Abe, Toshinori Okawa, Chie Wada -Mihara, 山本彩瑛 and Kenji Oka : Clinical case report of contributed QOL improvement wth esthetic partial prosthesis for missing maxillary incisor, 第16回日本総合歯科学会総会・学術大会, 58, Oct. 2023.
3.	山本彩瑛, Kenji Oka, Chihiro Shinohara, Susumu Abe, Chie Wada -Mihara and Toshinori Okawa : 2022 Clinical practice examination using the patient robot (SIMROID) at Tokushima University Hospital, 第16回日本総合歯科学会総会・学術大会, 43, Oct. 2023.
4.	Hinayo Umeda, Susumu Abe, Toshinori Okawa, Chie Wada -Mihara, Kenji Oka and Fumiaki Kawano : 徳島大学歯学部2年生を対象としたシャドウイング実習による学修行動変容の効果, 日本総合歯科学会, Nov. 2022.
5.	Toshinori Okawa, Susumu Abe, Kenji Oka, Chie Wada -Mihara, UMEDA hinayo and Fumiaki Kawano : A case of severe dentin hypersensitivity caused by steroid pulse therapy, 第15回日本総合歯科学会学術大会, Nov. 2022.
6.	Kenji Oka, Chihiro Shinohara, Susumu Abe, Chie Wada -Mihara, Toshinori Okawa and Fumiaki Kawano : Clinical practice examination using the patient robot(SIMROID®) at Tokushima University hospital, 第39回日本歯科医学教育学会総会および学術大会プログラム・抄録集, 81, Sep. 2020.
7.	Chie Wada -Mihara, Jun-ichi Kido, Masami Ninomiya and Hiromichi Yumoto : 薬物性歯肉増殖を伴う高齢の慢性歯周炎患者に対して包括的歯周治療を行った1症例, 第62回秋季日本歯周病学会学術大会, Oct. 2018.
8.	Chie Wada -Mihara, Jun-ichi Kido, Masami Ninomiya and Hiromichi Yumoto : A case report of comprehensive treatment for an elderly patient of chronic periodontitis with drug-induced gingival overgrowth, 第61回秋季日本歯周病学会学術大会, Vol.60, 153, Oct. 2018. (Keyword) Drug-induced gingival overgrowth / Calcium channel blockers / Chronic periodontitis
9.	Rie Kido, Eijiro Sakamoto, Mika Bandou, Yuji Inagaki, Masami Ninomiya, Chie Wada -Mihara, Takahisa Ikuta, Koji Naruishi, Toshihiko Nagata, Jun-ichi Kido and Hiromichi Yumoto : インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断, 第61回春季日本歯周病学会学術大会, Jun. 2018.
10.	Chie Wada -Mihara, Masami Ninomiya, Jun-ichi Kido and Toshihiko Nagata : 降圧薬由来の歯肉増殖を伴う重度歯周炎患者に対する歯周治療の効果, 日本歯周病学会中国四国3大学・日本臨床歯周病学会中国四国支部合同研修会, Nov. 2017. (Keyword) Antihypertensive drug / Drug-induced gingival hyperplasia / periodontitis
11.	Masami Ninomiya, Yuji Inagaki, Chie Wada -Mihara, Mika Bandou, Yukiko Nakajima, Eijiro Sakamoto, Ikuta Takahisa, Nishikawa Yasufumi, Jung-Hwan Lew, Rie Kido, Takagi Ryousuke, Nonaka Kouhei, Koji Naruishi, Jun-ichi Kido and Toshihiko Nagata : 新規歯周組織再生剤「リグロス歯科用液キット」の有効性, 四国歯学会第51回例会, Jun. 2017. (Keyword) periodontitis / fibroblast growth factor 2 / periodontal regeneration / Regroth
12.	Chie Wada -Mihara, Koji Naruishi, Mika Bandou, 西川泰史, Hwan Jung Lew, Eijiro Sakamoto, 生田貴久, 河野薫, Yukari Kajiura, 橋本万里, Yukiko Nakajima, Yuji Inagaki, Masami Ninomiya, Jun-ichi Kido and Toshihiko Nagata : アドバンスドOSCEを見据えた当科の模擬OSCEの概要, Shikoku Dental Research, Vol.29, No.2, 49-53, Jun. 2017.
13.	Chie Wada -Mihara, Jun-ichi Kido and Toshihiko Nagata : A case report of severe periodontitis with tooth movement induced by gingival overgrowth, Journal of the Japanese Association of Periodontology, Vol.58, No.9, 145, Oct. 2016.
14.	Chie Wada -Mihara : ラット実験的歯周炎におけるオステオプロテジェリンの局所投与による歯槽骨吸収抑制効果, 薫風会セミナー, Mar. 2014.
15.	Jun-ichi Kido, Mika Bandou, Yukiko Bandou, Yuji Inagaki, Yuka Hiroshima, 梶浦由加里, Chie Wada -Mihara, Hiromi Murata, 生田貴久, 篠原宏貴, Makoto Funaki and Toshihiko Nagata : 糖尿病関連歯周炎の診断マーカーとしての歯肉溝滲出液中グリコアルブミンとカルプロテクチンの有用性, 第56回日本糖尿病学会, May 2013.
16.	Eijiro Sakamoto, Chie Wada -Mihara, 生田貴久, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : ラット骨芽細胞の分化発現に及ぼす最終糖化産物とリポ多糖の影響, 第56回春季歯周病学会, May 2013.
17.	坂本英次郎, Chie Wada -Mihara, 生田貴久, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : ラット骨芽細胞の分化発現に及ぼす最終糖化産物とリポ多糖の影響, 第56回春季歯周病学会, May 2013.
18.	坂本英次郎, Chie Wada -Mihara, 生田貴久, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : ラット骨芽細胞の分化に及ぼす最終糖化産物とリポ多糖の影響, 第19回徳島骨代謝研究会, Nov. 2012.
19.	Eijiro Sakamoto, Chie Wada -Mihara, 生田貴久, Yuji Inagaki, Jun-ichi Kido and Toshihiko Nagata : ラット骨芽細胞の分化発現に及ぼす最終糖化産物及びリポ多糖の影響, 日本歯科保存学雑誌第136回春季学術大会講演抄録集, 82, Jun. 2012.
20.	Jun-ichi Kido, Yuji Inagaki, Mika Bandou, Yuka Hiroshima, Hiromi Murata, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, Masami Ninomiya, Keiji Oishi, Eijiro Sakamoto, 中島由紀子, 生田貴久, 浅原洋士 and Toshihiko Nagata : バイオマーカーを用いた糖尿病関連歯周炎の診断研究, Journal of the Japanese Association for Dental Science, Vol.31, 86, Mar. 2012. (Keyword) *生物学的マーカー
21.	Jun-ichi Kido, Yuji Inagaki, Mika Bandou, Yuka Hiroshima, Hiromi Murata, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, Masami Ninomiya, Keiji Oishi, Eijiro Sakamoto, 中島由紀子, 生田貴久, 浅原洋士 and Toshihiko Nagata : バイオマーカーを用いた糖尿病関連歯周炎の診断研究, Journal of the Japanese Association for Dental Science, Vol.31, 86, Mar. 2012. (Keyword) *生物学的マーカー
22.	Jun-ichi Kido, Yuji Inagaki, Mika Bandou, Yuka Hiroshima, Hiromi Murata, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, Masami Ninomiya, Keiji Oishi, 坂本英次郎, 中島由紀子, 生田貴久, 浅原洋士 and Toshihiko Nagata : バイオマーカーを用いた糖尿病関連歯周炎の診断研究, Journal of the Japanese Association for Dental Science, Vol.31, 86, Mar. 2012. (Keyword) *生物学的マーカー
23.	Chie Wada -Mihara, Jun-ichi Kido, Satoshi Yoneda, Yuka Hiroshima, Hiroyuki Seto and Toshihiko Nagata : 上顎前歯の著しいフレアアウトを伴った薬物性歯肉増殖症患者の一症例, Journal of the Japanese Association of Periodontology, May 2011.
24.	坂本英次郎, Chie Wada -Mihara, 徳永格, 瀬戸浩行 and Yuka Hiroshima : ラット実験的歯周炎におけるオステオプロテジェリンの影響, 第53回秋季日本歯周病学会高松, Sep. 2010.
25.	Yuji Inagaki, Jun-ichi Kido, Mika Bandou, Yuka Hiroshima and Chie Wada -Mihara : 糖尿病関連歯周炎における歯肉溝浸出液中のバイオマーカーの分析, 第53回日本糖尿病学会, May 2010.
26.	Jun-ichi Kido, Yuji Inagaki, Mika Bandou, Yuka Hiroshima, Masatoshi Kataoka, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, 坂本英次郎, 中島由紀子, Masami Ninomiya, Keiji Oishi, 淺原洋士 and Toshihiko Nagata : 歯肉溝浸出液中の糖尿病関連歯周炎バイオマーカーの検索, Journal of the Japanese Association of Periodontology, Vol.52, 97, May 2010.
27.	Yuji Inagaki, Jun-ichi Kido, Mika Bandou, Yuka Hiroshima, Masatoshi Kataoka, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, 坂本英次郎, 中島由紀子, Masami Ninomiya, Keiji Oishi, 淺原洋士 and Toshihiko Nagata : 糖尿病関連歯周炎における歯肉溝滲出液中のバイオマーカーの分析, Journal of the The Japan Diabetes Society, Vol.53, No.Suppl.1, S-145, Apr. 2010. (Keyword) 生物学的マーカー
28.	Yuka Hiroshima, Mika Bandou, Jun-ichi Kido, Yuji Inagaki, Chie Wada -Mihara, Keiji Oishi, Masatoshi Kataoka and Toshihiko Nagata : ヒト口腔上皮細胞における抗菌ペプチド遺伝子発現に及ぼす小柴胡湯の影響, 第131回日本歯科保存学会秋季学術大会, Oct. 2009.
29.	Chie Wada -Mihara, 徳永格, 瀬戸浩行, 大場博史, Yuka Hiroshima, Jun-ichi Kido and Toshihiko Nagata : ラット実験的歯周炎におけるオステオプロテジェリンの骨吸収と炎症におよぼす効果, 第52回秋季日本歯周病学会学術大会, Vol.51, 93, Oct. 2009.
30.	Mika Bandou, Yuka Hiroshima, Yuji Inagaki, Masatoshi Kataoka, Chie Wada -Mihara, Masumi Horibe, Satoshi Yoneda, Keiji Oishi, Masami Ninomiya, Javkhlan Purevjav, Jun-ichi Kido and Toshihiko Nagata : 糖尿病関連歯周炎における歯肉溝浸出液中バイオマーカーの分析, Journal of the Japanese Association of Periodontology, Vol.51, 101, Oct. 2009.
31.	Jun-ichi Kido, Masatoshi Kataoka, Mika Bandou, Yuka Hiroshima, Chie Wada -Mihara and Toshihiko Nagata : マイクロチップ基板を用いたカルプロテクチンの測定‐歯周病診断法への応用の可能性について‐, 第52回春季日本歯周病学会学術大会, Vol.51, 114, May 2009.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

糖尿病関連歯周炎の歯周治療による糖尿病改善効果を指背毛細血管を用いて可視化する (Project/Area Number: 21K09875 )
ファイブロティックアクティビティから薬物性歯肉増殖症の発症リスクを診断する (Project/Area Number: 24593124 )
An investigation of predictive marker of bisphosphonate-related osteonecrosis of the jaw. (Project/Area Number: 22792090 )
The possibility of osteoproteger in as a topical application drug inperiodontal therapy. (Project/Area Number: 20791615 )
薬物誘発性歯肉増殖症の遺伝子リスクファクターの検索 (Project/Area Number: 18791596 )
Search by Researcher Number (70403748)

Assistant Professor : Wada -Mihara, Chie

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)