Tokushima University / Educator and Researcher Directory --- GOTO, Masaki

Personal Web Page

https://researchmap.jp/masaki_goto (researchmap)

https://orcid.org/0000-0003-0494-4212 (ORCID)

Field of Study

○	Biophysical Chemistry

Subject of Study

○	脂質ナノ粒子(LNP)の高圧物性と産業的応用 (脂質二重膜, 界面活性剤, 高圧, 相転移, 蛍光イメージング, 放射光, 麻酔, 膜融合)

Book / Paper

Book:

1.	Masaki GOTO : 脂質の安定性(DSC)–水和結晶・非二重膜転移–, 熱量測定・熱分析ハンドブック第3版,(日本熱測定学会編集), 丸善出版, 東京, Aug. 2020.
2.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Encyclopedia of Biocolloid and Biointerface Science, --- Bilayer Imaging of Phosphatidylcholines by High-Pressure Fluorometry ---, John Wiley & Sons, Inc., Hoboken, Sep. 2016.
3.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Encyclopedia of Biocolloid and Biointerface Science, --- Phase Separation in Phospholipid Bilayers Induced by Cholesterol ---, John Wiley & Sons, Inc., Hoboken, Sep. 2016.
4.	Hitoshi Matsuki, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : 高圧バイオサイエンスとバイオテクノロジー, 三恵社, Nagoya, Nov. 2015.
5.	Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : 高圧力下の生物科学, さんえい出版株式会社, Kyoto, Nov. 2006.
6.	Kaori Tada, Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : 高圧力下の生物科学, さんえい出版株式会社, Kyoto, Nov. 2006.

Academic Paper (Judged Full Paper):

1.	Masaki GOTO, Shuntaro Yoshida, Shigeyuki Habara, Agnieszka Wilk-Kohlbrecher, Joachim Kohlbrecher, Nobutake Tamai and Hitoshi Matsuki : A molecular mechanism for how pressure induces interdigitation of phospholipid bilayer membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1866, No.8, 184385-1-184385-9, 2024. (Summary) The phase transition from the bilayer gel (ripple gel) phase to the nonbilayer gel (interdigitated gel) phase of bilayers of phosphatidylcholines (PCs) with two saturated long-chain fatty acids under high pressure was investigated by pressure-scanning microscopy, fluorometry, and dynamic light scattering (DLS) measurements. Microscopic observations for giant vesicles (GVs) of distearoyl-PC (DSPC) under high pressure showed that spherical GVs transforms into warped and distorted spherical ones instantaneously at the interdigitation. The fluorescence intensities of amphiphilic probe Prodan and hydrophobic probe Laurdan in the dipalmitoyl-PC (DPPC) bilayer steeply decreased and increased, respectively, at the interdigitation, suggesting that the conformational change of the polar head group of DPPC in the bilayer transiently occurred at the interdigitation. Further, the high-pressure DLS measurements revealed that the size of the vesicle particles of DPPC and DSPC transiently increased near the interdigitation pressure, whereas the ethanol-induced interdigitation of the DSPC bilayer under atmospheric pressure produced no such change in the particle size. Taking account of the critical packing parameter of the PC molecule, we could conclude that the pressure-induced interdigitation is attributable to the increase in repulsive interaction between the polar head groups of the PC molecules resulting from the orientational change of the head group from a parallel alignment to a perpendicular one with respect to the bilayer surface with pressure, namely the transient state: it occurs when the repulsive interaction exceeds a threshold value for the balance between the repulsive interaction and the attractive interaction among the hydrophobic acyl chains. (Keyword) Interdigitation / Non-bilayer / phase transition / Phospholipid / pressure (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2024.184385 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2024.184385 (DOI: 10.1016/j.bbamem.2024.184385)
2.	Nobutake Tamai, Hitoshi Matsuki and Masaki GOTO : Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence, Membranes, Vol.12, No.12, 1219-1-1219-17, 2022. (Summary) Prodan (6-propiponyl-2-(N,N-dimethylamino)naphthalene) is well known as a polarity-sensitive fluorescent probe and has a high capability of detecting structural changes occurring within phospholipid bilayer membranes. In this study, we carried out the fluorescence spectroscopic observation of bilayer phase behavior for a series of symmetric saturated diacylphosphatidylcholines (CnPCs) with different acyl-chain length n (n = 12 15 and 19 22) using Prodan as a membrane probe to confirm the availability of Prodan along with the previous results for the CnPC bilayer membranes (n = 16 18). The results were discussed by constructing spectral three-dimensional (3D) imaging plots for visualizing the change in bilayer phase states with temperature or pressure to verify the functionality of this 3D imaging plot. It was found that the Prodan fluorescence technique is applicable to the detection of the changes in the bilayer phase states of all CnPCs with a few exceptions. One of the most crucial exceptions was that Prodan cannot be used for the detection of the bilayer-gel state of the C21PC bilayer membrane. It was also found that it is only to the CnPC bilayer membranes with n = 15 18 that the 3D imaging plot is adequately and accurately applicable as a useful graphical tool for visually detecting the bilayer phase states. This is a disadvantageous feature of this technique brought about by the high sensitivity of Prodan as a membrane probe. Therefore, further detailed studies on the molecular behavior of Prodan will enable us to find a more useful way of utilizing this membrane probe. (Keyword) diacylphosphatidylcholine / Prodan (6-propiponyl-2-(N,N-dimethylamino)naphthalene) / bilayer membrane / phase transition / pressure (Tokushima University Institutional Repository) ● Metadata: 118067 (Link to Publication Site) ● Publication site (DOI): 10.3390/membranes12121219 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3390/membranes12121219 (Tokushima University Institutional Repository: 118067, DOI: 10.3390/membranes12121219)
3.	Toshiki Nakao, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : Temperature- and Pressure-Induced Bilayer Phase Transitions of an Amide-Linked Phosphatidylcholine: A Contrasting Effect of Chain Linkage Type, Bulletin of the Chemical Society of Japan, Vol.95, No.2, 261-270, 2022. (Summary) An unnatural phospholipid with two amide linkages, dipalmitoylamidodeoxyphosphatidylcholine (DPADPC), was synthesized and its bilayer phase transitions were observed by differential scanning calorimetry under atmospheric pressure and light-transmittance measurements under high pressure. The DPADPC bilayer membrane showed different phase transitions depending on the thermal history of the lipid sample under atmospheric pressure. The thermal-history dependent transitions were also observed under high pressure. The temperature pressure phase diagram and thermodynamic quantities of the phase transitions of the DPADPC bilayer membrane were compared with those of bilayer membranes of hydrophobic chain-linkage isomers, ester-liked dipalmitoylphosphatidylcholine (DPPC) and ether-linked dihexadecylphosphatidylcholine (DHPC). The thermodynamic quantities of the main transition for these PC bilayer membranes were similar to each other, whereas the suppression of the interdigitation and great stabilization of the hydrated crystal phase were observed for the DPADPC bilayer membrane. Furthermore, the results of nuclear magnetic resonance measurements indicated that the motility of PC molecules in both gel and liquid crystal phases of the DPADPC bilayer membranes were much lower than those of the DPPC and DHPC bilayer membranes. From the above results, we concluded that the molecular interaction in the PC bilayer membranes increases in the order of ether-, ester- and amide-linkage isomers. (Keyword) Amide-linked phospholipid / Lipid bilayer membrane / phase transition (Link to Publication Site) ● Publication site (DOI): 10.1246/bcsj.20210395 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1246/bcsj.20210395 (DOI: 10.1246/bcsj.20210395)
4.	Masaki GOTO, Akira Kazama, Kensuke Fukuhara, Honami Sato, Nobutake Tamai, Hiro-O Ito and Hitoshi Matsuki : Membrane fusion of phospholipid bilayers under high pressure: Spherical and irreversible growth of giant vesicles, Biophysical Chemistry, Vol.277, 106639-1-106639-11, 2021. (Summary) Membrane fusion of giant vesicles (GVs) for binary bilayers of unsaturated phospholipids, dioleoylphosphatidylethanolamine (DOPE) having an ability to promote membrane fusion and its homolog dioleoylphosphatidylcholine (DOPC) having an ability to form GV, was investigated under atmospheric and high pressure. While DOPC formed GVs in the presence of inorganic salts with a multivalent metal ion under atmospheric pressure, an equimolar mixture of DOPE and DOPC formed GVs both in the absence and the presence of LaCl3. We examined the change in size and shape of the GVs of this binary mixture in the absence and presence of LaCl3 as a function of time under atmospheric and high pressure. The size and shape of the GVs in the absence of LaCl3 under atmospheric and high pressure and those in the presence of LaCl3 under atmospheric pressure hardly changed with time. By contrast, the GV in the presence of LaCl3 under high pressure gradually changed in the size and shape with time on a time scale of several hours. Namely, the GV became larger than the original GV due to accelerated membrane fusion and its shape became more spherical. This pressure-induced membrane fusion was completely irreversible, and the growth rate was correlated with the applied pressure. The reason for the GV growth by applying pressure was considered on the basis of thermodynamic phase diagrams. We concluded that the growth is attributable to a closer packing of lipid molecules in the bilayer resulting from their preference of smaller volumes under high pressure. Furthermore, the molecular mechanism of the pressure-induced membrane fusion was explored by observing the fusion of two GVs with almost the same size. From their morphological changes, we revealed that the fusion is caused by the actions of Laplace and osmotic pressure. (Keyword) Giant vesicle / high pressure / Laplace pressure / Lipid bilayer membrane / Osmotic pressure / Membrane fusion (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bpc.2021.106639 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2021.106639 (DOI: 10.1016/j.bpc.2021.106639)
5.	Masaki GOTO, Sawaguchi Hiroshi, Nobutake Tamai and Hitoshi Matsuki : Subgel-phase formation of membranes of ether-linked phosphatidylcholines, Chemistry and Physics of Lipids, Vol.239, 105119-1-105119-8, 2021. (Summary) The formation of subgel (so-called hydrated crystal) phase of membranes of ether-linked phospholipids, dialkylphosphatidylcholines containing linear saturated alkyl chain (Cn = 14, 16 and 18), was examined under atmospheric and high pressure. The results of differential scanning calorimetry in 50 wt% aqueous ethylene glycol solution and water showed that these PC membranes undergo the subtransition from the subgel phase to the gel phase at a low temperature with or without the thermal pretreatment of lipid samples called annealing. The subtransition in water could be clearly observed by light-transmittance measurements under high pressure and the transition temperature increased by applying pressure. The temperature-pressure phase diagrams and the thermodynamic quantities of subtransition were obtained from the phase-transition data and compared with those of membranes of ester-linked phospholipids, diacylphosphatidylcholines. The phase diagrams indicated that all gel phases of the ether-linked PC membranes exist as stable phases while parts of the gel phases of the ester-linked PC membranes are metastable. The subtransition temperatures of the ether-linked PC membranes were lower than those of the ester-linked PC membranes by more than 10 °C and the corresponding thermodynamic quantities were extremely small. Further, it was revealed by high-pressure fluorometry that the difference in subgel phase between ether- and ester-linked PC membranes results from their phase structures: the nonbilayer interdigitated structure is maintained after the conversion from the gel phase to the subgel phase in the ether-linked PC membranes whereas the ester-linked PC membranes form bilayer subgel phase with staggered structure. (Keyword) Ether-linked phospholipid / Interdigitation / phase transition / Phospholipid membrane / pressure / Subgel phase (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2021.105119 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2021.105119 (DOI: 10.1016/j.chemphyslip.2021.105119)
6.	Hitoshi Matsuki, Masaki GOTO, Makiko Motohashi, Aoi Kiguchi, Toshiki Nakao and Nobutake Tamai : Formation of intermediate gel-liquid crystalline phase on medium-chain phosphatidylcholine bilayers: Phase transitions depending on the bilayer packing, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1862, No.5, 183197-1-183197-11, 2020. (Summary) The bilayer phase transitions of medium-chain phosphatidylcholines with linear saturated acyl chains (Cn = 12, 13 and 14) were measured by high-pressure light-transmittance measurements and differential scanning calorimetry to investigate the formation of intermediate gel-liquid crystalline phase called Lx phase. The constructed phase diagrams showed that there existed a distinct region of the Lx phase between ripple gel (Pβ') and liquid crystalline (Lα) phases for multilamellar vesicle bilayers of C12PC and C13PC. The Lx phase of the C12PC bilayer was metastable at all pressures and disappeared at a higher pressure. In the C13PC bilayer, the Lx phase was stable and also disappeared at a higher pressure but its region markedly shrunk. By contrast, the Lx phase was not detected for the C14PC bilayer. Effects of other factors such as vesicle size and solvent substitution on the Lx phase of the C13PC bilayer were also examined. A decrease in vesicle size and solvent substitution from water to 50 wt% ethylene glycol solution promoted the Lx-phase formation as opposed to the effects of acyl-chain elongation and pressurization. The fluorescence data of the C13PC bilayer with different vesicle sizes showed that the Lx phase is caused by the difference of local packing in the bilayer. Considering these facts, we concluded that the Lx phase is an intermediate gel-Lα phase that has gel-phase monolayers with negative curvature and Lα-phase monolayers with positive curvature. The formation mechanism of the Lx-phase in stacked bilayers and dispersed vesicles is also explainable by this difference in packing state. (Keyword) Bilayer Membrane / Intermediate gel-liquid crystalline phase / Packing state / phase transition / Phosphatidylcholine / pressure (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2020.183197 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2020.183197 (DOI: 10.1016/j.bbamem.2020.183197)
7.	Masaki GOTO, Hirotsugu Okamoto, Nobutake Tamai, Kazuhiro Fukada and Hitoshi Matsuki : Salt effect on bilayer phase transitions of dipalmitoylphosphatidylglycerol in saline water under high pressure, High Pressure Research, Vol.39, No.2, 238-247, 2019. (Summary) The phase transitions of dipalmitoylphosphatidylglycerol (DPPG) bilayer membrane at high NaCl concentrations under high pressure were investigated to construct the temperature pressure phase diagram and determine phase-transition properties. The constructed phase diagrams exhibited the qualitative resemblance to that of the dipalmitoylphosphatidylcholine (DPPC) bilayer membrane: they underwent the gel-phase polymorphism including the pressure-induced bilayer interdigitation. The phase-transition properties of the DPPG bilayer membrane changed in a salt concentration-dependent manner, whereas qualitatively the phase behavior is not affected appreciably by the salt concentration measured. We discussed the salt effect on the DPPG bilayer membrane from the variation in interactions between the polar head group of the PG molecule. (Keyword) Bilayer Membrane / Dipalmitoylphosphatidylglycerol / Interdigitation / Phase transition / Pressure / Prodan fluorescence (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2019.1601189 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85063877150 (DOI: 10.1080/08957959.2019.1601189, Elsevier: Scopus)
8.	Nobutake Tamai, Sanae Inazawa, Saori Takeuchi, Masaki GOTO and Hitoshi Matsuki : Phase Behavior of Binary Bilayer Membrane of Dipalmitoylphosphatidylcholine and Stigmasterol, Journal of Thermal Analysis and Calorimetry, Vol.135, No.5, 2635-2645, 2019. (Summary) We investigated the phase behavior of a binary bilayer membrane of dipalmitoylphosphatidylcholine (DPPC) and stigmasterol (Stig) as a function of the Stig concentration (XSt) by means of differential scanning calorimetry (DSC) and fluorescence spectroscopy using 6-propionyl-2-(N,N-dimethylamino)naphthalene (Prodan) as a membrane probe. The DSC results revealed that the pretransition and the main transition are abolished at XSt = ca. 0.08 and ca. 0.50, respectively. The main transition enthalpy DeltaHm of the DPPC Stig binary bilayer decreased with XSt in a non-linear manner, which is contrast to the almost linear decrease in DeltaHm for the DPPC cholesterol (Chol) binary bilayer. This suggests that Stig has a stronger effect of disturbing the chain packing in a gel state and a weaker effect of suppressing the chain melting as compared to Chol. The XSt dependence of the peak width at half height (DeltaT1/2) suggests that a drastic change in the phase behavior occurs at XSt = ca. 0.14, which is similar to the DPPC Chol binary bilayer. The Prodan fluorescence spectra changed depending on the phase state, which was characterized by the wavelength at the emission maximum and allowed us to determine the transition temperatures of the binary bilayers. The compositional phase diagram of the DPPC Stig binary bilayer was found to be similar to that of the DPPC Chol binary bilayer. The phase behavior of the DPPC Stig binary bilayer could be well explained by applying the so-called superlattice view to interpret the phase diagram. (Keyword) cholesterol / Incongruent melting / Lipid bilayer / Liquid ordered phase / phase diagram / Stigmasterol (Link to Publication Site) ● Publication site (DOI): 10.1007/s10973-018-7597-9 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85051429077 (DOI: 10.1007/s10973-018-7597-9, Elsevier: Scopus)
9.	Nobutake Tamai, Maiko Uemura, Takuya Izumikawa, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Phase Behavior of Cholesterol-Containing Binary Membrane of an Ether-Linked Phospholipid, Dihexadecylphosphatidylcholine, Colloid and Polymer Science, Vol.296, No.4, 697-711, 2018. (Summary) The thermotropic phase behavior of the cholesterol (Chol)-containing binary membrane of dihexadecylphosphatidylcholine (DHPC) was investigated as a function of the mole fraction of Chol Xch by DSC and fluoresce spectroscopy using 6-propionyl-2-(N,N-dimethylamino)naphthalene (Prodan). The main-transition enthalpy Hm was almost constant at Xch < ca. 0.05, but it decreased almost linearly with increasing Xch above 0.05 until it reached 0 at Xch = ca. 0.50, suggesting that the interaction between DHPC and Chol is relatively weaker than that between dipalmitoylphosphatidylcholine (DPPC) and Chol. The Prodan fluorescence spectra changed depending on the phase state, which was characterized by the wavelength at the emission maximum. Particularly, the two-phase state of the interdigitated (LβI) and non-interdigitated gel (Lβ) was sensitively detected as the superposition of the two elementary spectra corresponding to each phase state, allowing us to explain the Xch dependent non-linear behavior of the Chol-induced Lβ phase by introducing a proportionality factor ξ(Xch) and by assuming a simple probabilistic process. By comparing the phase diagram constructed for the DHPC Chol binary membrane with that for the DPPC Chol binary bilayer, a difference was observed in the minimum Xch value for the entire formation of the liquid ordered (Lo) phase. This difference could be explained in terms of the hydrophobic mismatch at the bilayer midplane by taking into account the difference in the capability of hydrogen bonding with the 3β-OH group of Chol due to the presence or absence of the carbonyl oxygens at the glycerol backbone. (Keyword) cholesterol / Dihexadecylphosphatidylcholines / Interdigitation / Liquid ordered phase / phase diagram / Peritectic point (Link to Publication Site) ● Publication site (DOI): 10.1007/s00396-018-4280-4 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/s00396-018-4280-4 (DOI: 10.1007/s00396-018-4280-4)
10.	Hitoshi Matsuki, Kentaro Kato, Hirotsugu Okamoto, Shuntaro Yoshida, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Ligand partitioning into lipid bilayer membranes under high pressure: implication of variation in phase-transition temperatures, Chemistry and Physics of Lipids, Vol.209, 9-18, 2017. (Summary) The variation in phase-transition temperatures of dipalmitoylphosphatidylcholine (DPPC) bilayer membrane by adding two membrane-active ligands, a long-chain fatty acid (palmitic acid (PA)) and an inhalation anesthetic (halothane (HAL)), was investigated by light-transmittance measurements and fluorometry. By assuming the thermodynamic colligative property for the bilayer membrane at low ligand concentrations, the partitioning behavior of these ligands into the DPPC bilayer membrane was considered. It was proved from the differential partition coefficients between two phases that PA has strong affinity with the gel (lamellar gel) phase in a micro-molal concentration range and makes the bilayer membrane more ordered while HAL has strong affinity with the liquid crystalline phase in a milli-molal concentration range and does the bilayer membrane more disordered. The transfer volumes of both ligands from the aqueous solution to each phase of the DPPC bilayer membrane showed that the preferential partitioning of the PA molecule into the gel (lamellar gel) produces about 20% decrease in transfer volume as compared with the liquid crystalline phase, whereas that of the HAL molecule into the liquid crystalline phase does about twice increase in transfer volume as compared with the gel (ripple gel) phase. Furthermore, changes in thermotropic and barotropic phase behavior of the DPPC bilayer membrane by adding the ligand was discussed from the viewpoint of the ligand partitioning. Reflecting the contrastive partitioning of PA and HAL into the pressure-induced interdigitated gel phase among the gel phases, it was revealed that PA suppresses the formation of the interdigitated gel phase under high pressure while HAL promotes it. These results clearly indicate that each phase of the DPPC bilayer membrane has a potential to recognize various ligand molecules. (Keyword) High pressure / Inhalation anesthetic / Ligand partitioning / Lipid bilayer membrane / Long-chain fatty acid / Phase transition (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2017.10.002 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2017.10.002 (DOI: 10.1016/j.chemphyslip.2017.10.002)
11.	Masaki GOTO, Yuya Aoki, Nobutake Tamai and Hitoshi Matsuki : Effect of pressure on bilayer phase behavior of N-methylated di-O-hexadecylphosphatidylethanolamines: relevance of head-group modification on the bilayer interdigitation, Biophysical Chemistry, Vol.231, 64-70, 2017. (Summary) The phase transitions of N-methylated di-O-hexadecylphosphatidylethanolamines (DHPE, DH-N-methyl-PE (DHMePE) and DH-N,N-dimethyl-PE (DHMe2PE)) were observed by differential scanning calorimetry (DSC) and fluorometry under atmospheric pressure and light-transmittance measurements under high pressure. The DSC thermograms showed that the N-methylated DHPE bilayers underwent the phase transitions from the gel phase to the liquid crystalline (Lα) phase under atmospheric pressure. The gel phase was identified by fluorometry as the lamellar gel (Lβ) phase, and not interdigitated gel (LβI) phase. The gel/Lα transition temperature increased with pressure while decreased stepwise with increasing polar head-group size. This stepwise depression in the transition temperature may be caused by the inverse-proportional hydrogen-bonding capabilities of the head-group to the head-group size. The thermodynamic quantities of the gel/Lα transition were comparable for the N-methylated DHPE bilayers. The pressure-induced LβI phase was not found in these bilayers although the bilayer of di-O-hexadecylphosphatidylcholine (DHPC), which is a kind of N-methylated DHPEs, forms the LβI phase only by hydration under atmospheric pressure. Taking into account that the bilayers of diacyl-homologs of N-methylated DHPEs, N-methylated dipalmitoyl-PEs except for dipalmitoylphosphatidylcholine (DPPC), do not form the LβI phase in the whole pressure range investigated but the DPPC bilayer forms the LβI phase under high pressure, we can say that the interdigitation requires a weaker interaction between large-sized head groups like the bulky choline group. (Keyword) Bilayer interdigitation / Ether-linked phospholipid / Lipid bilayer membrane / Phase transition / Pressure (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bpc.2017.03.009 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2017.03.009 (DOI: 10.1016/j.bpc.2017.03.009)
12.	Hitoshi Matsuki, Shigeru Endo, Ryosuke Sueyoshi, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Thermotropic and barotropic phase transitions on diacylphosphatidylethanolamine bilayer membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1859, No.7, 1222-1232, 2017. (Summary) The bilayer phase transitions of four diacylphosphatidylethanolamines (PEs) with identical two saturated acyl chains (Cn = 12, 14, 16 and 18) and two PEs with identical two unsaturated acyl chains containing a different kind of double bonds were observed by differential scanning calorimetry under atmospheric pressure and light-transmittance measurements under high pressure. The temperature-pressure phase diagrams for these PE bilayer membranes were constructed from the obtained phase-transition data. The saturated PE bilayer membranes underwent two different phase transitions related to the liquid crystalline (Lα) phase, the transition from the hydrated crystalline (Lc) phase and the chain melting (main) transition, depending on the thermal history. Pressure induced the change in the gel-phase stability of the bilayer membranes of PEs with longer chains at a low pressure. Comparing the thermodynamic quantities of the saturated PE bilayer membranes with those of diacylphosphatidylcholines (PC) bilayer membranes, the PE bilayer membranes showed higher phase-transition temperatures and formed more stable Lc phase, which originates from the strong interaction between polar head groups of PE molecules. On the other hand, the unsaturated PE bilayer membranes underwent the transition from the Lα phase to the inverted hexagonal (HII) phase at a high temperature and this transition showed small thermodynamic quantities but high-pressure responsivity. It turned out that the kind of double bonds markedly affects both bilayer-bilayer and bilayer-nonbilayer transitions and the Lα/HII transition is a volume driven transition for the reconstruction of molecular packing. Further, the phase-transition behavior was explained by chemical potential profiles of bilayer phases. (Keyword) Differential scanning calorimetry / Lipid bilayer membrane / Phase stability / Phase transition / Phosphatidylethanolamine / Pressure (Tokushima University Institutional Repository) ● Metadata: 111087 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2017.03.020 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28366514 ● Search Scopus @ Elsevier (PMID): 28366514 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2017.03.020 (Tokushima University Institutional Repository: 111087, DOI: 10.1016/j.bbamem.2017.03.020, PubMed: 28366514)
13.	Masaki GOTO, Takuya Endo, Takahiro Yano, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : Comprehensive characterization of temperature- and pressure-induced bilayer phase transitions for saturated phosphatidylcholines containing longer chain homologs, Colloids and Surfaces B:Biointerfaces, Vol.128, 389-397, 2015. (Summary) Complete elucidation of the phase behavior of phospholipid bilayers requires information on the subtransition from the lamellar crystal (Lc) phase to the gel phase. However, for bilayers of saturated diacylphosphatidylcholines (CnPCs), especially longer chain homologs, equilibration in the Lc phase is known to be very slow. In this study, bilayer phase transitions of three CnPCs with longer acyl chains, C19PC, C20PC and C21PC, were observed by differential scanning calorimetry under atmospheric pressure and by light-transmittance measurements under high pressure. Using lipid samples treated by thermal annealing enabled the observation of the sub-, pre- and main transitions of the C19PC and C20PC bilayers under atmospheric pressure. Only the pre- and main transitions could be observed for the C21PC bilayer due to very slow kinetics of the Lc phase formation for lipids with long acyl chains. The temperature and pressure phase diagrams constructed and phase-transitions quantities (enthalpy, entropy and volume changes) evaluated for these bilayers were compared with one another and with those of bilayers of the CnPC homologs examined in previous studies. These results allowed us (1) to clarify the temperature- and pressure-dependent phase sequence and phase stability of the CnPC (n = 12 22) bilayers as a function of the hydrophobicity of the molecules, (2) to prove the presence of a shorter and a longer limit (n = 13 and 21) in the acyl-chain length for the pressure-induced bilayer interdigitation and (3) to reveal the chain-length dependence of the thermodynamic quantities of the subtransitions including the volume change. (Keyword) Bilayer membrane / Diacylphosphatidylcholine / Interdigitation / phase transition / high pressure / Subgel phase (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2015.02.036 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84938086188 (DOI: 10.1016/j.colsurfb.2015.02.036, Elsevier: Scopus)
14.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Pressure-induced interdigitation of lipid bilayer membranes: Dependence on Acyl-chain length and limitation of the formation, Journal of the Society of Materials Science, Japan, Vol.63, No.8, 620-623, 2014. (Summary) We investigated the bilayer interdigitation of diacylphosphatidylcholines (CnPCs) with long acyl chains (n = 19 - 22) under high pressure. The temperature-pressure phase diagrams of the CnPC bilayers were constructed by the methods of differential scanning calorimetry and high-pressure light transmittance. We confirmed the bilayer interdigitation for CnPCs (n = 19 - 21) under high pressure. On the other hand, the C22PC bilayer showed peculiar behavior: two transitions were observed under atmospheric pressure while one transition under high pressure. To identify the low-temperature phase of the C22PC bilayer, we performed small angle neutron scattering measurements under atmospheric pressure and fluorescence measurements under high pressure. From these results, the phase was identified as the interdigitated gel (LβI) phase. Considering our previous results, it turned out that the acyl-chain length of CnPC that exhibits the pressure-induced interdigitation is limited within n = 14 - 21. The present results definitely indicate that the bilayer interdigitation is governed by the hydrophobic interaction as well as the hydrophilic interaction of the PC molecule in the bilayer. (Keyword) Bilayer phase transition / bilayer phase transition / Lipid bilayers / lipid bilayers / Phase behavior / phase behavior / Pressure-induced interdigitation / pressure-induced interdigitation / Saturated and unsaturated phospholipids / saturated and unsaturated phospholipids (Link to Publication Site) ● Publication site (DOI): 10.2472/jsms.63.620 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282680421512704 ● Summary page in Scopus @ Elsevier: 2-s2.0-84906962918 (DOI: 10.2472/jsms.63.620, CiNii: 1390282680421512704, Elsevier: Scopus)
15.	Nobutake Tamai, Takuya Izumikawa, Suguru Fukui, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : How Does Acyl-chain Length Affect Thermotropic Phase Behavior of Saturated Diacylphosphatidylcholine-chloresterol Binary Bilayers?, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1828, No.11, 2513-2523, 2013. (Summary) Thermotropic phase behavior of diacylphosphatidylcholine (CnPC) cholesterol binary bilayers (n = 14 16) was examined by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry. The former technique can detect structural changes of the bilayer in response to the changes in polarity around Prodan molecules partitioned in a relatively hydrophilic region of the bilayer, while the latter is sensitive to the conformational changes of the acyl chains. On the basis of the data from both techniques, we propose possible temperature T cholesterol composition Xch phase diagrams for these binary bilayers. A notable feature of our phase diagrams, including our previous results for C17PC and C18PC, is that there is a peritectic-like point around Xch = 0.15, which can be interpreted as indicating the formation of a 1:6-complex of cholesterol and CnPCs within the binary bilayer irrespective of the acyl chain length. We could give a reasonable explanation for such complex formation using the modified superlattice view. Our results also showed that the Xch value of the abolition of the main transition is almost constant for n = 14 17 (ca. 0.33), while it increases to ca.0.50 for n = 18. By contrast, a biphasic n-dependence of Xch was observed for the abolition of the pretransition, suggesting that there are at least two antagonistic n-dependent factors. We speculate that this could be explained by the enhancement of the van der Waals interaction with increases in n and the weakening of the repulsion between the neighboring headgroups with decreases in n. (Keyword) cholesterol / Diacylphosphatidylcholines / Peritectic point / phase diagram / Lateral phase separation / Superlattice (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2013.06.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23791704 ● Search Scopus @ Elsevier (PMID): 23791704 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2013.06.008 (DOI: 10.1016/j.bbamem.2013.06.008, PubMed: 23791704)
16.	Nobutake Tamai, Sayuri Kakibe, Tanaka Saeko, Masaki GOTO and Hitoshi Matsuki : An Attempt to Reveal Viscoelastic Behavior of Lipid Bilayer Membrane by Pressure Perturbation Calorimetry, High Pressure Research, Vol.33, No.2, 271-277, 2013. (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2013.767900 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2013.767900 (DOI: 10.1080/08957959.2013.767900)
17.	Nobutake Tamai, Sanae Inazawa, Daiki Fujiwara, Masaki GOTO and Hitoshi Matsuki : Thermotropic Phase Behavior of Binary Bilayer Membrane of Dipalmitoylphosphatidylcholine and Ergosterol, Chemistry Letters, Vol.41, No.10, 1087-1089, 2012. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.2012.1087 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2012.1087 (DOI: 10.1246/cl.2012.1087)
18.	Masaki GOTO, Agnieszka Wilk, Kosuke Kataoka, Shirish Chodankar, Nobutake Tamai, Makoto Fukui, Joachim Kohlbrecher, Hiro-O Ito and Hitoshi Matsuki : Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry, Langmuir, Vol.28, No.33, 12191-12198, 2012. (Summary) The myristoylpalmitoylphosphatidylcholine (MPPC) bilayer membrane shows a complicated temperature-pressure phase diagram. The large portion of the lamellar gel (L(β)'), ripple gel (P(β)'), and pressure-induced gel (L(β)I) phases exist as metastable phases due to the extremely stable subgel (L(c)) phase. The stable L(c) phase enables us to examine the properties of the L(c) phase. The phases of the MPPC bilayers under atmospheric and high pressures were studied by small-angle neutron scattering (SANS) and fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan. The SANS measurements clearly demonstrated the existence of the metastable L(β)I phase with the smallest lamellar repeat distance. From a second-derivative analysis of the fluorescence data, the line shape for the L(c) phase under high pressure was characterized by a broad peak with a minimum of ca. 460 nm. The line shapes and the minimum intensity wavelength (λ''(min)) values changed with pressure, indicating that the L(c) phase has highly pressure-sensible structure. The λ''(min) values of the L(c) phase spectra were split into ca. 430 and 500 nm in the L(β)I phase region, which corresponds to the formation of a interdigitated subgel L(c) (L(c)I) phase. Moreover, the phase transitions related to the L(c) phase were reversible transitions under high pressure. Taking into account the fluorescence behavior of Prodan for the L(c) phase, we concluded that the structure of the L(c) phase is highly probably a staggered structure, which can transform into the L(c)I phase easily. (Keyword) 2-Naphthylamine / Atmospheric Pressure / Cell Membrane / Fluorometry / Gels / Lipid Bilayers / Neutron Diffraction / Phosphatidylcholines / Pressure / Scattering, Small Angle (Link to Publication Site) ● Publication site (DOI): 10.1021/la3020173 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22823885 ● Summary page in Scopus @ Elsevier: 2-s2.0-84865495527 (DOI: 10.1021/la3020173, PubMed: 22823885, Elsevier: Scopus)
19.	Saeko Tanaka, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer Membrane in Saline Water under High Pressure, International Journal of Modern Physics: Conference Series, Vol.6, 727-732, 2012. (Link to Publication Site) ● Publication site (DOI): 10.1142/S2010194512004059 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1142/S2010194512004059 (DOI: 10.1142/S2010194512004059)
20.	Nobutake Tamai, Maiko Tsutsui, Masaki GOTO and Hitoshi Matsuki : Volumetric Study on Dioleoylphosphatidylcholine Bilayer by Pressure Perturbation Calorimetry, International Journal of Modern Physics: Conference Series, Vol.6, 762-767, 2012. (Link to Publication Site) ● Publication site (DOI): 10.1142/S2010194512004114 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1142/S2010194512004114 (DOI: 10.1142/S2010194512004114)
21.	Nobutake Tamai, Yuko Nambu, Saeko Tanaka, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Volumetric characterization of ester- and ether-linked lipid bilayers by pressure perturbation calorimetry and densitometry, Colloids and Surfaces B:Biointerfaces, Vol.92, No.1, 232-239, 2012. (Summary) We investigated the thermotropic volume behavior of dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and dihexadecylphosphatidylcholine (DHPC) membranes using pressure perturbation calorimetry (PPC) and densitometry. The ln φ(2) vs temperature curves (φ(2): apparent molar volume of phospholipid) obtained from the PPC data using an analysis method that we developed agreed with the results from the density measurements for these lipids within the relative difference of about 0.62%. From those curves, the volume changes with the main transition were estimated at 18.0±0.49, 23.5±2.33 and 23.0±0.33 cm(3) mol(-1) for DMPC, DPPC and DHPC, respectively. For DPPC and DMPC, the average volume per methylene group of the hydrocarbon chains v(CH2) calculated by referring to the procedure by Nagle and Wilkinson was consistent with the previous result, which indicates that the DPPC bilayer in the gel state has denser hydrophobic bilayer core than the DMPC bilayer. For DHPC, the volume of the headgroup region v(H) was calculated to be 244 Å(3) by assuming that v(CH2) of DHPC equals that of DPPC above 45°C. This value was comparable to that of DPPC when the volume of the carbonyl groups was considered, which may signify that there is no significant conformational difference in the polar headgroups of both phospholipids. However, it was suggested from the consideration on v(H) of DHPC at 20°C that expansion of the headgroup region should occur as the interdigitated structure is formed, which means some conformational change of the headgroup region is induced by the interdigitation. (Keyword) 1,2-Dipalmitoylphosphatidylcholine / Calorimetry / Densitometry / Dimyristoylphosphatidylcholine / Esters / Ether, Ethyl / Lipid Bilayers / Phospholipid Ethers / Pressure / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2011.11.055 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22221457 ● Search Scopus @ Elsevier (PMID): 22221457 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2011.11.055 (DOI: 10.1016/j.colsurfb.2011.11.055, PubMed: 22221457)
22.	Saeko Tanaka, Nobutake Tamai, Masaki GOTO, Shoji Kaneshina and Hitoshi Matsuki : Morphological Change of Vesicle Particle can Produce a Peculir Stepwise Transition in Dipalmitoylphosphatidylglycerol Bilayer at High NaCl Concentration, Chemistry Letters, Vol.41, No.3, 304-306, 2012. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.2012.304 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1360283694088387200 ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2012.304 (DOI: 10.1246/cl.2012.304, CiNii: 1360283694088387200)
23.	Hitoshi Matsuki, Masaki GOTO, Masataka Kusube and Nobutake Tamai : Imaging of Phosphatidylcholine Bilayers by a High-Pressure Fluorescence Technique: Detection of the Packing Difference, Bulletin of the Chemical Society of Japan, Vol.84, No.12, 1329-1335, 2011. (Summary) The packing states in phosphatidylcholine (PC) bilayers under high pressure were studied from fluorescence measurements using a polarity-sensitive probe Prodan. By using the Prodan fluorescence data, three-dimensional image plots, which can visually evaluate the bilayer packing, were constructed for the C16PC bilayer. It turned out from the shape of contours in the image plot constructed by the second-derivative spectra that the Prodan molecules can be distributed into multiple sites in the bilayer and move around the head-group region depending on the phase state. Furthermore, comparing the plot to those for the C17PC and C18PC bilayers, the slight difference in hydrophobicity among the PC molecules produced a marked difference in quantity of the Prodan molecules distributed among the bilayer gel, nonbilayer gel, and liquid crystalline phases of the PC membranes. The present study shows that the imaging using Prodan is useful for the evaluation of lipid bilayer packing. (Keyword) General Chemistry (Link to Publication Site) ● Publication site (DOI): 10.1246/bcsj.20110229 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1360283694073547136 ● Search Scopus @ Elsevier (DOI): 10.1246/bcsj.20110229 (DOI: 10.1246/bcsj.20110229, CiNii: 1360283694073547136)
24.	Tselmeg Baatarjav, Kosuke Kataoka, Rebekah S. Gilbert, Yutaka Terao, Makoto Fukui, Masaki GOTO, Shigetada Kawabata, Masato Yamamoto, Kohtaro Fujihashi and Hiro-O Ito : Mucosal immune features to phosphorylcholine by nasal Flt3 ligand cDNA-based vaccination, Vaccine, Vol.29, No.34, 5747-5757, 2011. (Summary) Phosphorylcholine (PC) is an immunodominant epitope in some pathogens including Streptococcus pneumoniae and it is well-known that PC-specific antibodies (Abs) play a key role in the induction of protective immunity against pneumococcal infection. In this study, we examined whether nasal administration of DNA plasmid encoding Flt3 ligand gene (pFL) as a mucosal adjuvant plus PC-conjugated keyhole limpet hemocyanin (PC-KLH), would elicit PC-specific immune responses, and characterized mucosal immune responses to PC induced by this nasal vaccination. Nasal immunization with pFL plus PC-KLH enhanced induction of PC-specific IgA and IgM Abs in airway secretions when compared with mice given PC-KLH with or without empty plasmid gene (pORF) as controls; in addition to the mucosal immune responses, PC-specific immune responses in serum were also induced. Furthermore, the mucosal and serum IgA and IgM Abs in mice given pFL plus PC-KLH nasally, exhibited high-specificity for the PC molecule. Of interest, the PC-specific Abs bound dose-dependently to anti-T15 idiotype (AB1-2). Thus, the inhibition of S. pneumoniae colonization on the nasal cavity and lungs after nasal challenge with the live organism was significantly elicited in mice immunized with pFL plus PC-KLH compared to that of mice immunized with antigen with pORF. Taken together, these findings show that nasal administration of pFL with PC-KLH elicited T15-like anti-PC IgA and IgM Abs in the respiratory tracts, and further attenuated S. pneumoniae colonization on the respiratory tracts. Nasal administration of Flt3 ligand cDNA with PC may contribute to the development of nasal vaccination for prevention of S. pneumoniae infection. (Keyword) Administration, Intranasal / Administration, Mucosal / Animals / Antibodies, Bacterial / Bacterial Vaccines / DNA, Complementary / Hemocyanin / Immunity, Mucosal / Immunoglobulin A / Immunoglobulin M / Membrane Proteins / Mice / Mice, Inbred C57BL / Phosphorylcholine / Plasmids / Pneumococcal Infections / Streptococcus pneumoniae / Vaccines, DNA (Link to Publication Site) ● Publication site (DOI): 10.1016/j.vaccine.2011.05.097 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21683111 ● Summary page in Scopus @ Elsevier: 2-s2.0-79960560899 (DOI: 10.1016/j.vaccine.2011.05.097, PubMed: 21683111, Elsevier: Scopus)
25.	Masaki GOTO, Shunsuke Ishida, Yuka Ito, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Thermotropic and Barotropic Phase Transitions of Dialkyldimethylammonium Bromide Bilayer Membranes: Effect of Chain Length, Langmuir, Vol.27, No.10, 5824-5831, 2011. (Summary) The bilayer phase transitions of dialkyldimethylammonium bromides (2C(n)Br; n = 12, 14, 16) were observed by differential scanning calorimetry and high-pressure light-transmittance measurements. Under atmospheric pressure, the 2C(12)Br bilayer membrane underwent the stable transition from the lamellar crystal (L(c)) phase to the liquid crystalline (L(α)) phase. The 2C(14)Br bilayer underwent the main transition from the metastable lamellar gel (L(β)) phase to the metastable L(α) phase in addition to the stable L(c)/L(α) transition. For the 2C(16)Br bilayer, moreover, three kinds of phase transitions were observed: the metastable main transition, the metastable transition from the metastable lamellar crystal (L(c(2))) phase to the metastable L(α) phase, and the stable lamellar crystal (L(c(1)))/L(α) transition. The temperatures of all the phase transitions elevated almost linearly with increasing pressure. The temperature (T)-pressure (p) phase diagrams of the 2C(12)Br and 2C(14)Br bilayers were simple, but that of the 2C(16)Br bilayer was complex; that is, the T-p curves for the metastable main transition and the L(c(2))/L(α) transition intersect at ca. 25 MPa, which means the inversion of the relative phase stability between the metastable phases of L(β) and L(c(2)) above and below the pressure. Moreover, the T-p curve of the L(c(2))/L(α) transition was separated into two curves under high pressure, and as a result, the pressure-induced L(c(2P)) phase appeared in between. Thermodynamic quantities for phase transitions of the 2C(n)Br bilayers increased with an increase in alkyl-chain length. The chain-length dependence of the phase-transition temperature for all kinds of transitions observed suggests that the stable L(c(1))/L(α) transition incorporates the metastable L(c(2))/L(α) transition in the bilayers of 2C(n)Br with shorter alkyl chains, and the main-transition of the 2C(12)Br bilayer would occur at a temperature below 0 °C. (Keyword) Atmospheric Pressure / Cell Membrane / Light / Lipid Bilayers / Phase Transition / Quaternary Ammonium Compounds / Surface-Active Agents / Temperature / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1021/la200323h (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21520909 ● Search Scopus @ Elsevier (PMID): 21520909 ● Search Scopus @ Elsevier (DOI): 10.1021/la200323h (DOI: 10.1021/la200323h, PubMed: 21520909)
26.	Masaki GOTO, Takayuki Matsui, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Prodan fluorescence detects the bilayer packing of asymmetric phospholipids, Colloids and Surfaces B:Biointerfaces, Vol.84, No.1, 55-62, 2011. (Summary) The bilayer phase behavior of asymmetric phospholipids, palmitoylstearoylphosphatidylcholine (PSPC) and stearoylpalmitoylphosphatidylcholine (SPPC), with different vesicle sizes (large multilamellar vesicle (LMV) and giant multilamellar vesicle (GMV)) was investigated by fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan under high pressure. The results were compared with those of a symmetric phospholipid, diheptadecanoyl PC (C17PC). The difference in phase transitions of the PSPC and SPPC bilayers and in thermodynamic quantities of the transitions was hardly observed between LMV and GMV as the case of the C17PC bilayer. On the other hand, the Prodan fluorescence showed clear differences between LMV and GMV of the asymmetric PC bilayers. From the second derivative of Prodan fluorescence spectra, the three dimensional image plots in which we can clearly see the location of Prodan in the bilayer membrane as blue valleys were constructed for LMV and GMV under high pressure. We revealed from the plots that the bilayer packing is significantly dependent on not only the vesicle size but also the acyl-chain asymmetry of PC molecule in addition to the phase states. It was found that the packing of the gel phases of the asymmetric PC bilayers is weaker than that of the symmetric PC bilayer, and the size of vesicle affects the packing of the interdigitated gel phase the most markedly among three gel phases. This study suggests that the Prodan molecules can detect the effect of vesicle size on the phase states for the asymmetric PC bilayers, and they become a useful indicator for various membrane properties, especially bilayer interdigitation. (Keyword) 2-Naphthylamine / Fluorescence / Fluorescent Dyes / Lipid Bilayers / Models, Biological / Phase Transition / Phospholipids / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2010.12.015 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21232926 ● Search Scopus @ Elsevier (PMID): 21232926 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2010.12.015 (DOI: 10.1016/j.colsurfb.2010.12.015, PubMed: 21232926)
27.	Masaki GOTO, Agnieszka Wilk, Akira Kazama, Shirish Chodankar, Joachim Kohlbrecher and Hitoshi Matsuki : Chain elongation of diacylphosphatidylcholine induces fully bilayer interdigitation under atmospheric pressure, Colloids and Surfaces B:Biointerfaces, Vol.84, No.1, 44-48, 2011. (Summary) The phase transitions of dibehenoylphosphatidylcholine (C22PC) bilayer membrane were observed by differential scanning calorimetry under atmospheric pressure and light-transmittance measurements under high pressure. The constructed temperature-pressure phase diagram suggests that the gel phase at low temperatures is the interdigitated gel phase. To confirm the phase state, we performed small-angle neutron scattering and fluorescence measurements using a polarity-sensitive probe Prodan for the C22PC bilayer membrane under atmospheric pressure. The peaks obtained in both measurements clearly showed the characteristic patterns of the fully interdigitated gel phase. Taking into account of previous studies on the gel phase for long-chain PC bilayers under atmospheric pressure and our studies on the pressure-induced bilayer interdigitaion of diacyl-PCs, it turned out that the interdigitation of diacyl-PC bilayer membranes occurs when the carbon number of acyl chain reaches at least 22. The present study revealed that the interdigitation of PC bilayer membranes occurs not only by weakening the attractive force of polar head groups but also by strengthening the cohesive force of acyl chains. When dominating the force of acyl chains, the interdigitation can be induced even in a diacyl-PC bilayer membrane by only hydration under atmospheric pressure. (Keyword) Atmospheric Pressure / Lipid Bilayers / Models, Biological / Phosphatidylcholines (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2010.12.010 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21216568 ● Search Scopus @ Elsevier (PMID): 21216568 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2010.12.010 (DOI: 10.1016/j.colsurfb.2010.12.010, PubMed: 21216568)
28.	Masaki GOTO, Yuka Ito, Shunsuke Ishida, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Hydrostatic Pressure Reveals Bilayer Phase Behavior of Dioctadecyldimethylammonium Bromide and Chloride, Langmuir, Vol.27, No.5, 1592-1598, 2011. (Summary) Bilayer phase transitions of dioctadecyldimethylammonium bromide (2C(18)Br) and chloride (2C(18)Cl) were observed by differential scanning calorimetry and high-pressure light-transmittance measurements. The 2C(18)Br bilayer membrane showed different kinds of transitions depending on preparation methods of samples under atmospheric pressure. Under certain conditions, the 2C(18)Br bilayer underwent three kinds of transitions, the metastable transition from the metastable lamellar crystal (L(c(2))) phase to the metastable lamellar gel (L(β)) phase at 35.4 °C, the metastable main transition from the metastable L(β) phase to the metastable liquid crystalline (L(α)) phase at 44.5 °C, and the stable transition from the stable lamellar crystal (L(c(1))) phase to the stable L(α) phase at 52.8 °C. On the contrary, the 2C(18)Cl bilayer underwent two kinds of transitions, the stable transition from the stable L(c) phase to the stable L(β) phase at 19.7 °C and the stable main transition from the stable L(β) phase to the stable L(α) phase at 39.9 °C. The temperatures of the phase transitions of the 2C(18)Br and 2C(18)Cl bilayers were almost linearly elevated by applying pressure. It was found from the temperature (T)-pressure (p) phase diagram of the 2C(18)Br bilayer that the T-p curves for the main transition and the L(c(1))/L(α) transition intersect at ca. 130 MPa because of the larger slope of the former transition curve. On the other hand, the T-p phase diagram of the 2C(18)Cl bilayer took a simple shape. The thermodynamic properties for the main transition of the 2C(18)Br and 2C(18)Cl bilayers were comparable to each other, whereas those for the L(c(1))/L(α) transition of the 2C(18)Br bilayer showed considerably high values, signifying that the L(c(1)) phase of the 2C(18)Br bilayer is extremely stable. These differences observed in both bilayers are attributable to the difference in interaction between a surfactant and its counterion. (Keyword) Atmospheric Pressure / Hydrostatic Pressure / Phase Transition / Quaternary Ammonium Compounds / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1021/la104552z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21261314 ● Search Scopus @ Elsevier (PMID): 21261314 ● Search Scopus @ Elsevier (DOI): 10.1021/la104552z (DOI: 10.1021/la104552z, PubMed: 21261314)
29.	Nobutake Tamai, Yuko Nambu, Maiko Tsutsui, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Application of pressure perturbation calorimetry to the aqueous system of phospholipid vesicle dispersion, High Pressure Research, Vol.30, No.4, 490-498, 2010. (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2010.526115 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2010.526115 (DOI: 10.1080/08957959.2010.526115)
30.	Hitoshi Matsuki, Makoto Nishimoto, Kaori Tada, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Packing state in bilayer membranes of diacylphosphatidylcholines with varying acyl chain lengths under high pressure, High Pressure Research, Vol.30, No.4, 475-482, 2010. (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2010.526114 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2010.526114 (DOI: 10.1080/08957959.2010.526114)
31.	Masaki GOTO, Hiroshi Sawaguchi, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Effect of Vesicle Size on the Prodan Fluorescence in Diheptadecanoylphosphatidylcholine Bilayer Membrane under Atmospheric and High Pressures, Langmuir, Vol.26, No.16, 13377-13384, 2010. (Summary) The bilayer phase behavior of diheptadecanoylphosphatidylcholine (C17PC) with different vesicle sizes (large multilamellar vesicle (LMV) and giant multilamellar vesicle (GMV)) was investigated by fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan under atmospheric and high pressures. The difference in phase transitions and thermodynamic quantities of the transition was hardly observed between LMV and GMV used here. On the contrary, the Prodan fluorescence in the bilayer membranes changed depending on the size of vesicles as well as on the phase states. From the second derivative of fluorescence spectra, the three-dimensional image plots in which we can see the location of Prodan in the bilayer membrane as blue valleys were constructed for LMV and GMV under atmospheric pressure. The following characteristic behavior was found: (1) the Prodan molecules in GMV can be distributed to not only adjacent glycerol backbone region, but also near bulk-water region in the lamellar gel or ripple gel phase; (2) the blue valleys of GMV became deeper than those of LMV because of the greater surface density of the Prodan molecules per unit area of GMV than LMV; (3) the liquid crystalline phase of the bilayer excludes the Prodan molecules to a more hydrophilic region at the membrane surface with an increase in vesicle size; (4) the accurate information as to the phase transitions is gradually lost with increasing vesicle size. Under the high-pressure condition, the difference in Prodan fluorescence between LMV and GMV was essentially the same as the difference under atmospheric pressure except for the existence of the pressure-induced interdigitated gel phase. Further, we found that Prodan fluorescence spectra in the interdigitated gel phase were especially affected by the size of vesicles. This study revealed that the Prodan molecules can move around the headgroup region by responding not only to the phase state but also to the vesicle size, and they become a useful membrane probe, detecting important membrane properties such as the packing stress. (Keyword) 2-Naphthylamine / Fluorescent Dyes / Lipid Bilayers / Phosphatidylcholines / Pressure / Spectrometry, Fluorescence / Temperature / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1021/la100871z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20695581 ● Search Scopus @ Elsevier (PMID): 20695581 ● Search Scopus @ Elsevier (DOI): 10.1021/la100871z (DOI: 10.1021/la100871z, PubMed: 20695581)
32.	Masataka Kusube, Takumi Nishino, Yuki Nishikawa, Masaki GOTO, Hitoshi Matsuki and Hitoshi Iwahashi : Recovery of Tobacco BY-2 Cells After Treatment of High Hydrostatic Pressure, Annals of the New York Academy of Sciences, Vol.1189, 139-142, 2010. (Summary) The recovery of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells in Linsmaire and Skoog medium after treatment at high hydrostatic pressure was investigated using an Evans Blue staining method to discriminate live from dead cells. The survival of BY-2 cells just after the high-pressure treatment at 5 degrees C and 25 degrees C decreased abruptly at pressures higher than 50 MPa and 100 MPa, respectively. Furthermore, almost all of the BY-2 cells treated at 5 degrees C and 25 degrees C recovered pressures below 25 MPa and 75 MPa, respectively. However, no BY-2 cells recovered at pressures above 100 MPa at either temperature. (Keyword) Biotechnology / Cell Line / Cell Proliferation / Cell Survival / Hydrostatic Pressure / Preservation, Biological / Temperature / Tobacco (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1749-6632.2009.05185.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233380 ● Search Scopus @ Elsevier (PMID): 20233380 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05185.x (DOI: 10.1111/j.1749-6632.2009.05185.x, PubMed: 20233380)
33.	Masaki GOTO, Masataka Kusube, Makoto Nisimoto, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Pressure Study on Symmetric and Asymmetric Phospholipid Bilayers: Effect of Vesicle Size on Prodan Fluorescence, Annals of the New York Academy of Sciences, Vol.1189, 68-76, 2010. (Summary) The bilayer phase behavior of symmetric and asymmetric phosphatidylcholines (PCs), 1,2-diheptadecanoyl-PC (C17PC), 1-palmitoyl-2-stearoyl-PC (PSPC), and 1-stearoyl-2-palmitoyl-PC (SPPC), with different vesicle sizes were investigated by a high-pressure fluorescence method using the polarity-sensitive fluorescent probe Prodan. The second derivative of fluorescence spectra for all the PCs of small-sized vesicle showed four minima characteristic of four membrane states on the spectra irrespective of the acyl-chain symmetry, whereas those of large-sized vesicle had one more minimum originating from the most hydrophilic site at the membrane surface. These findings indicate that Prodan molecules can distribute into multiple sites in the bilayer and move around the head-group region depending on the vesicle size. The behavior of the spectra in the SPPC bilayer suggested that the interdigitated gel phase had a less polar "pocket" formed by a space between uneven terminal methyl ends of the sn-1 and sn-2 chains. It turned out that the curvature of vesicles affects the distribution of the Prodan molecules in all phases, more particularly in the interdigitated gel phase. (Keyword) 2-Naphthylamine / Biotechnology / Fluorescent Dyes / Hydrostatic Pressure / Lipid Bilayers / Particle Size / Phase Transition / Phosphatidylcholines / Phosphatidylserines / Phospholipids / Spectrometry, Fluorescence (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1749-6632.2009.05202.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233370 ● Search Scopus @ Elsevier (PMID): 20233370 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05202.x (DOI: 10.1111/j.1749-6632.2009.05202.x, PubMed: 20233370)
34.	Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Pressure Effect on the Bilayer Phase Transition of Asymmetric Lipids with an Unsaturated Acyl Chain, Annals of the New York Academy of Sciences, Vol.1189, 77-85, 2010. (Summary) The bilayer phase transitions of mixed-chain lipids with monounsaturated acyl chain in the sn-2 position, 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), and with a polyunsaturated acyl chain in the sn-2 position, 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (SDPC), were observed by differential scanning calorimetry (DSC) under ambient pressure and by light-transmittance measurements under high pressure. The DSC thermogram for each lipid bilayer showed only one transition between the lamellar gel and liquid crystalline phases. The introduction of one or two cis double bonds into the sn-2 acyl chain caused the significant depression of the main-transition temperature and an obvious decrease of enthalpy and volume changes associated with the transition. These features are attributable to loose packing of saturated and unsaturated acyl chains in the bilayer gel phase. The existence of four or six double bonds in the sn-2 chain produced no further decrease in the transition temperature, and in fact six double bonds caused a slight increase in the transition temperature. Thermodynamic properties associated with the bilayer phase transition were discussed. (Keyword) Biotechnology / Calorimetry, Differential Scanning / Fatty Acids, Monounsaturated / Fatty Acids, Unsaturated / Gels / Hydrostatic Pressure / Lipid Bilayers / Molecular Structure / Phase Transition / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1749-6632.2009.05203.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233371 ● Search Scopus @ Elsevier (PMID): 20233371 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05203.x (DOI: 10.1111/j.1749-6632.2009.05203.x, PubMed: 20233371)
35.	Ismail Ismaliza Mohd Nor, Tsuyoshi Yuasa, Keizo Yuasa, Yuko Nambu, Makoto Nishimoto, Masaki GOTO, Hitoshi Matsuki, Masahiro Inoue, Masami Nagahama and Akihiko Tsuji : A critical role for highly conserved Glu⁶¹⁰ residue of oligopeptidase B Tryoanosoma brucei in thermal stability, The Journal of Biochemistry, Vol.147, No.2, 201-211, 2010. (Summary) Oligopeptidase B from Trypanosoma brucei (Tb OPB) is a virulence factor and therapeutic target in African sleeping sickness. Three glutamic acid residues at positions 607, 609 and 610 of the catalytic domain are highly conserved in the OPB subfamily. In this study, the roles of Glu(607), Glu(609) and Glu(610) in Tb OPB were investigated by site-directed mutagenesis. A striking effect on k(cat)/K(m) was obtained following mutation of Glu(607) to glutamine. In contrast, the heat stability of Tb OPB decreased markedly following the single mutation of Glu(610) to glutamine, although this mutation had significantly less effect on catalytic properties compared with the Glu(607) mutation. Although no differences were found in the tertiary and secondary structures between wild-type (WT) OPB and the E610Q mutant prior to heat treatment, the E610Q mutant is inactivated more rapidly than WT OPB following heat treatment in a manner correlating with its attendant structural changes. Trypsin digestion showed that the boundary regions between the beta-propeller and catalytic domain of the E610Q mutant are unfolded with heat treatment. It is concluded that Glu(607) is essential for the catalytic activity of Tb OPB and that Glu(610) plays a critical role in stabilization rather than catalytic activity despite their close proximity. (Keyword) Adrenocorticotropic Hormone / Animals / Calorimetry, Differential Scanning / Circular Dichroism / Enzyme Activation / Enzyme Stability / Glucagon / Glutamic Acid / Hot Temperature / Mutagenesis, Site-Directed / Mutation / Protozoan Proteins / Salts / Serine Endopeptidases / Trypanosoma brucei brucei / Trypsin / Urea (Link to Publication Site) ● Publication site (DOI): 10.1093/jb/mvp156 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19819899 ● Search Scopus @ Elsevier (PMID): 19819899 ● Search Scopus @ Elsevier (DOI): 10.1093/jb/mvp156 (DOI: 10.1093/jb/mvp156, PubMed: 19819899)
36.	Agnieszka Broniec, Masaki GOTO and Hitoshi Matsuki : A Peculiar Phase Transition of Plasmalogen Bilayer Membrane under High Pressure, Langmuir, Vol.25, No.19, 11265-11268, 2009. (Summary) The bilayer phase transition of plasmalogen, monounsaturated plasmenylcholine 1-O-1'-(Z)-octadecenyl-2-oleoyl-sn-glycero-3-phosphocholine (Plg-SOPC), was examined by differential scanning calorimetry, high-pressure transmittance, and fluorescence techniques. The bilayer properties of Plg-SOPC such as the temperature-pressure phase diagram, the thermodynamic quantities of the transition, and the location of a fluorescent membrane probe in the bilayer, were compared with those of a similar phospholipid 1-stearoyl-2-oleoyl-phosphatidylcholine (SOPC). It turned out that a vinyl-ether bond in the sn-1 position of the glycerol backbone in the Plg-SOPC molecule produces a peculiar phase transition under high pressure and significantly affects the membrane properties. (Keyword) Cell Membrane / Light / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Plasmalogens / Pressure / Scattering, Radiation / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1021/la902503n (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19697955 ● Search Scopus @ Elsevier (PMID): 19697955 ● Search Scopus @ Elsevier (DOI): 10.1021/la902503n (DOI: 10.1021/la902503n, PubMed: 19697955)
37.	Masaki GOTO, Shunsuke Ishida, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Chain asymmetry alters thermotropic and barotropic properties of phospholipid bilayer membranes, Chemistry and Physics of Lipids, Vol.161, No.2, 65-76, 2009. (Summary) The alignment of the sn-1 and sn-2 acyl chains at the terminal methyl ends generally produces significant influence on the thermodynamic properties of the bilayer phase transitions. We investigated the bilayer phase behavior of asymmetric phospholipids, myristoylpalmitoylphosphatidylcholine and palmitoylmyristoylphosphatidylcholine, by high-pressure light-transmittance and Prodan-fluorescence techniques and differential scanning calorimetry. Constructed temperature-pressure phase diagrams revealed that no stable Lbeta' phase can exist in the whole pressure range because of the formation of the most stable Lc phase. Nevertheless, the pretransition, the detection of which is severely hampered by the exceptionally prompt formation of the Lc phase, was successfully observed. Moreover, the effect of the total and difference of the sn-1 and sn-2 acyl chain lengths on minimal interdigitation pressure (MIP) was summarized in a MIP vs. chain-length inequivalence parameter plot, where the effect was proved to be classified in three zones depending on the alignment of both terminal methyl ends. (Keyword) 2-Naphthylamine / Calorimetry, Differential Scanning / Cholesterol / Hydrostatic Pressure / Lipid Bilayers / Phase Transition / Phospholipids / Pressure / Structure-Activity Relationship / Temperature / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2009.07.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19616525 ● Search Scopus @ Elsevier (PMID): 19616525 ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2009.07.003 (DOI: 10.1016/j.chemphyslip.2009.07.003, PubMed: 19616525)
38.	Kaori Tada, Eri Miyazaki, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Barotropic and thermotropic bilayer phase behavior of positional isomers of unsaturated mixed-chain phosphatidylcholines, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1788, No.5, 1056-1063, 2009. (Summary) The bilayer phase transitions of six kinds of mixed-chain phosphatidylcholines (PCs) with an unsaturated acyl chain in the sn-1 or sn-2 position, 1-oleoyl-2-stearoyl- (OSPC), 1-stearoyl-2-oleoyl- (SOPC), 1-oleoyl-2-palmitoyl- (OPPC), 1-palmitoyl-2-oleoyl- (POPC), 1-oleoyl-2-myristoyl- (OMPC) and 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC), were observed by means of differential scanning calorimetry (DSC) and high-pressure light transmittance measurements. Bilayer membranes of SOPC, POPC and MOPC with an unsaturated acyl chain in the sn-2 position exhibited only one phase transition, which was identified as the main transition between the lamellar gel (L(beta)) and liquid crystalline (L(alpha)) phases. On the other hand, the bilayer membranes of OSPC, OPPC and OMPC with an unsaturated acyl chain in the sn-1 position exhibited not only the main transition but also a transition from the lamellar crystal (L(c)) to the L(beta) (or L(alpha)) phase. The stability of their gel phases was markedly affected by pressure and chain length of the saturated acyl chain in the sn-2 position. Considering the effective chain lengths of unsaturated mixed-chain PCs, the difference in the effective chain length between the sn-1 and sn-2 acyl chains was proven to be closely related to the temperature difference of the main transition. That is, a mismatch of the effective chain length promotes a temperature difference of the main transition between the positional isomers. Anomalously small volume changes of the L(c)/L(alpha) transition for the OPPC and OMPC bilayers were found despite their large enthalpy changes. This behavior is attributable to the existence of a cis double bond and to significant inequivalence between the sn-1 and sn-2 acyl chains, which brings about a small volume change for chain melting due to loose chain packing, corresponding to a large partial molar volume, even in the L(c) phase. Further, the bilayer behavior of unsaturated mixed-chain PCs containing an unsaturated acyl chain in the sn-1 or sn-2 position was well explained by the chemical-potential diagram of a lipid in each phase. (Keyword) Biophysical Phenomena / Calorimetry, Differential Scanning / Ethylene Glycol / Isomerism / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2009.02.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19233121 ● Summary page in Scopus @ Elsevier: 2-s2.0-65249097838 (DOI: 10.1016/j.bbamem.2009.02.008, PubMed: 19233121, Elsevier: Scopus)
39.	Makoto Nishimoto, Takashi Hata, Masaki GOTO, Nobutake Tamai, Shoji Kaneshina, Hitoshi Matsuki and Issaku Ueda : Interaction modes of long-chain fatty acids in dipalmitoylphosphatidylcholine bilayer membrane: contrast to mode of inhalation anesthetics, Chemistry and Physics of Lipids, Vol.158, No.2, 71-80, 2009. (Summary) The effects of long-chain fatty acids (four saturated and two unsaturated fatty acids, one derivative) on phase transitions of dipalmitoylphosphatidylcholine (DPPC) bilayer membranes were examined in the low concentration region, and the results were compared with those for an inhalation anesthetic. The effects of all fatty acids on the pre- and main-transition temperatures of the DPPC bilayer membrane appeared in the concentration range of microM order while that of the anesthetic appeared in the mM order. The appearance modes of these ligand actions were significantly different from one another. The three differential partition coefficients of the ligands between two phases of the DPPC bilayer membrane were evaluated by applying the thermodynamic equation to the variation of the phase-transition temperatures. The DPPC bilayer membranes showed the different receptivity for the ligands; the saturated fatty acids had an affinity for gel phase whereas unsaturated fatty acids and an anesthetic had an affinity for liquid-crystalline phase to the contrary. In particular, the receptivity for the ligands in the gel phase markedly changed depending on kinds of ligands. The interaction modes between the DPPC and fatty acid molecules in the gel phase were considered from the hexagonal lattice model. The disappearance compositions of the pretransition by the fatty acids coincided with the compositions at which the membrane is all covered by the units in each of which two fatty acids molecules are regularly distributed in the hexagonal lattice in a different way, and the distribution depended on the chain length and existence of a double bond for the fatty acids. The interpretation did not hold for the case of the anesthetic at all, which proved that a number of anesthetic molecules act the surface region of the bilayer membrane nonspecifically. The present study clearly implies that DPPC bilayer membranes have high ability to recognize kinds of ligand molecules and can discriminate among them with specific interaction by the membrane states. (Keyword) 1,2-Dipalmitoylphosphatidylcholine / Anesthetics / Calorimetry, Differential Scanning / Fatty Acids / Lipid Bilayers / Phase Transition (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2009.02.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19428351 ● Search Scopus @ Elsevier (PMID): 19428351 ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2009.02.003 (DOI: 10.1016/j.chemphyslip.2009.02.003, PubMed: 19428351)
40.	Kaori Tada, Kensuke Saito, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : High-pressure study on bilayer phase behavior of oleoylmyristoyl- and myristoyloleoyl-phosphatidylcholines, Biophysical Chemistry, Vol.138, No.1-2, 36-41, 2008. (Summary) We investigated the thermotropic and barotropic bilayer phase behavior of 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC) and 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine (OMPC) by means of the differential scanning calorimetry (DSC) and high-pressure light-transmittance technique. Water could be used as a solvent for measurements at high pressures because of the elevation of the transition temperatures above 0 degrees C by pressurization, whereas aqueous 50 wt.% ethylene glycol solution was used mainly for those at low pressures. Only one phase transition was observed in the DSC thermogram of the MOPC bilayer membrane as an endothermic peak, and also observed at high pressures as an abrupt change of the light-transmittance. The transition was assigned as a main transition between the lamellar gel (L(beta)) and liquid-crystalline (L(alpha)) phases on the basis of the values of enthalpy change (DeltaH) and slope of the transition temperature with respect to pressure (dT/dP). The DSC thermogram of the OMPC bilayer membrane similarly showed a single endothermic peak but two kinds of phase transitions were observed at different temperatures in the light-transmittance profile at high pressures. The extrapolation of the lower-temperature transition in the high-pressure range to an ambient pressure coincided with the transition observed in the DSC thermogram. This transition was identified as a transition between the lamellar crystal (L(c)) and L(alpha) (or L(beta)) phases from the DeltaH and dT/dP values. The higher-temperature transition, appearing only at high pressures, was identified as the L(beta)/L(alpha) transition considering the topological resemblance of its temperature-pressure phase diagram as that of the dioleoylphosphatidylcholine bilayer membrane. The phase diagram of the OMPC bilayer membrane demonstrated that the L(beta) phase cannot exist at pressures below ca. 190 MPa while it can exist stably in a finite temperature range at pressures above the pressure. (Keyword) Calorimetry, Differential Scanning / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Temperature / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bpc.2008.08.009 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18804320 ● Search Scopus @ Elsevier (PMID): 18804320 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2008.08.009 (DOI: 10.1016/j.bpc.2008.08.009, PubMed: 18804320)
41.	Nobutake Tamai, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Lateral phase separation in cholesterol/diheptadecanoylphosphatidylcholine binary bilayer membrane, Colloids and Surfaces B:Biointerfaces, Vol.65, No.2, 213-219, 2008. (Summary) We investigated the phase behavior of cholesterol/diheptadecanoylphosphatidylcholine (C17:0-PC) binary bilayer membrane as a function of the cholesterol composition (X(ch)) by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry (DSC). The fluorescence spectra showed that the wavelength at the maximum intensity (lambda(max)) changed depending on the bilayer state: ca. 440 nm for the lamellar gel (L'(beta) or L(beta)) and the liquid ordered (L(o)) phases and ca. 490 nm for the liquid-crystalline (L(alpha)) phase. The transition temperatures were determined from the temperature dependence of lambda(max) and endothermic peaks of the DSC thermograms. Both measurements showed that the pre- and main transition disappear around X(ch)=0.05 and 0.30, respectively. The constructed temperature-X(ch) phase diagram resembled a typical phase diagram for a eutectic binary mixture containing a peritectic point. The presence of a peritectic point at X(ch)=0.15 suggested that a complex of cholesterol and C17:0-PC is stoichiometrically formed in the gel phase. Consideration based on the hexagonal lattice model revealed that the compositions of 0.05 and 0.15 correspond to the bilayer states where cholesterol molecules are regularly distributed in different ways. The former is nearly equal to the composition for the membrane occupied entirely with Units (1:18), composed of a cholesterol and 18 surrounding C17:0-PC molecules within the next-next nearest neighbor sites. The latter is represented by a Unit (1:6), including a cholesterol and 6 surrounding C17:0-PC molecules. Further, the disappearance of the main transition at X(ch)=0.30 indicates that the pure L(o) phase can exist in X(ch)>0.30. The eutectic behavior observed in the phase diagram was explainable in terms of phase separation between two different types of regions with different types of regular distributions of cholesterol. (Keyword) Calorimetry, Differential Scanning / Cholesterol / Crystallization / Lipid Bilayers / Phosphatidylcholines / Spectrometry, Fluorescence (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2008.04.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18502622 ● Search Scopus @ Elsevier (PMID): 18502622 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2008.04.004 (DOI: 10.1016/j.colsurfb.2008.04.004, PubMed: 18502622)
42.	Shoji Kaneshina, Masaki GOTO, 多田佳織, Nobutake Tamai and Hitoshi Matsuki : エーテル結合型リン脂質における指組み構造形成, High Pressure Bioscience and Biotechnology, Vol.2, No.1, 75-81, 2008.
43.	Masaki GOTO, 戸田雅隆, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : 高圧力下におけるジノナデカノイルホスファチジルコリン二分子膜の相挙動, High Pressure Bioscience and Biotechnology, Vol.2, No.1, 68-74, 2008. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.2.68 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.68 (DOI: 10.11229/hpbb.2.68)
44.	Hitoshi Matsuki, 多田佳織, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Thermodynamic Consideration for Nonbilayer Formation of Phospholipid Membranes, High Pressure Bioscience and Biotechnology, Vol.2, No.1, 61-67, 2008. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.2.61 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.61 (DOI: 10.11229/hpbb.2.61)
45.	Nobutake Tamai, Maiko Uemura, Tetsuya Takeichi, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : A new interpretation of eutectic behavior for distearoylphosphatidylcholine-cholesterol binary bilayer membrane, Biophysical Chemistry, Vol.135, No.1-3, 95-101, 2008. (Summary) We investigated the thermotropic phase behavior of the distearoylphosphatidylcholine (DSPC)-cholesterol binary bilayer membrane as a function of the cholesterol composition (X(ch)) by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry (DSC). The fluorescence spectra, each of which has a single maximum, showed that the wavelength at the maximum intensity (lambda(max)) changed depending on the bilayer state: ca. 440 nm for the lamellar gel (L(beta)' or L(beta)) and the liquid ordered (L(o)) phases, ca. 470 nm for the ripple gel (P(beta)') phase and ca. 490 nm for the liquid crystalline (L(alpha)) phase, respectively. The transition temperatures were determined from the temperature dependences of the lambda(max) and endothermic peaks of the DSC thermograms. Both measurements showed that the pretransition disappears around X(ch)=0.035. The constructed temperature-X(ch) phase diagram indicated that the phase behavior of the binary bilayer membrane at X(ch)< or =0.15 is similar to that of general liquid-solid equilibrium for a binary system where both components are completely miscible in the liquid phase and completely immiscible in the solid phase. It was also revealed that the diagram has two characteristic points: a congruent melting point at X(ch)=0.08 and a peritectic-like point at X(ch)=0.15. The hexagonal lattice model was used for the interpretation of the phase behavior of the binary bilayer membrane. These characteristic compositions well correspond to the bilayer states in each of which cholesterol molecules are regularly distributed in the hexagonal lattice in a different way. That is, each composition of 0.035, 0.08 and 0.15 is nearly equal to that for the binary bilayer membrane which is entirely occupied with units, each composed of a cholesterol and 30 surrounding DSPC molecules within the next-next-next nearest neighbor sites (Unit (1:30): L(beta)(1:30)), with units, each of a cholesterol and 12 surrounding DSPC molecules within the next nearest sites (Unit (1:12): L(beta)(1:12)) or with units, each of a cholesterol and 6 surrounding DSPC molecules at the nearest neighbor sites (Unit (1:6): L(beta)(1:6)), respectively. Therefore, the eutectic behavior observed in the phase diagram was fully explainable in terms of a kind of phase separation between two different types of regions with different types of regular distributions of cholesterol. Further, the L(o) phase was found in the higher X(ch)-region (X(ch)>0.15). No endothermic peak over the temperature range from 10 to 80 degrees C at X(ch)=0.50 suggested that the single L(o) phase can exist at X(ch)>0.50. (Keyword) Calorimetry, Differential Scanning / Cholesterol / Fluorescence / Fluorescent Dyes / Lipid Bilayers / Models, Biological / Phase Transition / Phosphatidylcholines / Thermodynamics / Transition Temperature (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bpc.2008.03.008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18436366 ● Search Scopus @ Elsevier (PMID): 18436366 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2008.03.008 (DOI: 10.1016/j.bpc.2008.03.008, PubMed: 18436366)
46.	Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Thermotropic and barotropic phase transitions of dilauroylphosphatidylcholine bilayer, Chemistry and Physics of Lipids, Vol.153, No.2, 138-143, 2008. (Summary) The bilayer phase transitions of dilauroylphosphatidylcholine (DLPC), containing two linear acyl chains with 12 carbon atoms, were observed by means of differential scanning calorimetry (DSC) under ambient pressure and light transmittance under high pressure. When the heating scan for the DLPC bilayer in 50 wt.% aqueous ethylene glycol (EG) solution began at -30 degrees C after cold storage, the DSC thermogram showed two endothermic peaks at 1.7 and 4.5 degrees C, which correspond to the transition from the lamellar crystalline (Lc) phase to the intermediate liquid crystalline (Lx) phase and the transition from the Lx phase to the liquid crystalline (L) phase, respectively. Extremely large enthalpy change (32.9 kJ mol(-1)) is characteristic of the Lc/Lx phase transition. The DSC thermogram for the heating scan beginning from -10 degrees C showed a single endothermic peak with 9.2 kJ mol(-1) at -0.4 degrees C, which was assigned as the so-called main transition between the metastable ripple gel (P'(beta)) and metastable Lalpha phases. The DLPC bilayer under high pressure underwent three kinds of transitions in EG solution, whereas only one transition was observed in water under high pressure. The middle-temperature transition in EG solution could be assigned to the main transition because of its consistency with the main transition in water. The lower-temperature transition is probably assigned as transition from the Lc phase to the P'(beta) phase. Since the slope (dT/dp) of the Lc/P'(beta) phase boundary is smaller than that for the main transition, the Lc/P'(beta) phase boundary and the main transition curves crossed each other at 40 MPa on the temperature-pressure phase diagram. The higher-temperature transition in EG solution refers to the transition from the Lx phase to the Lalpha phase. The Lx phase disappeared at about 180 MPa, and the direct transition from the P'(beta) phase to the Lalpha phase was observed at high pressures above 180 MPa. (Keyword) Calorimetry, Differential Scanning / Ethylene Glycol / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2008.03.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18394426 ● Search Scopus @ Elsevier (PMID): 18394426 ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2008.03.004 (DOI: 10.1016/j.chemphyslip.2008.03.004, PubMed: 18394426)
47.	Nobutake Tamai, Takayuki Matsui, Nobutaka Moribayashi, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Cholesterol Suppresses Pressure-induced Interdigitation of Dipalmitoylphosphatidylcholine Bilayer Membrane, Chemistry Letters, Vol.37, No.6, 604-605, 2008. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.2008.604 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1360283694087874304 ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2008.604 (DOI: 10.1246/cl.2008.604, CiNii: 1360283694087874304)
48.	Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Effect of hydrostatic pressure on the bilayer phase behavior of symmetric and asymmetric phospholipids with the same total chain length, Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1778, No.4, 1067-1078, 2008. (Summary) The bilayer phase transitions of palmitoylstearoyl-phosphatidylcholine (PSPC), diheptadecanoyl-PC (C17PC) and stearoylpalmitoyl-PC (SPPC) which have the same total carbon numbers in the two acyl chains were observed by differential scanning calorimetry and high-pressure optical method. As the temperature increased, these bilayers exhibited four phases of the subgel (Lc), lamellar gel (L beta'), ripple gel (P beta') and liquid crystal (L alpha), in turn. The Lc phase was observed only in the first heating scan after cold storage. The temperatures of the phase transitions were almost linearly elevated by applying pressure. The temperature-pressure phase diagrams and the thermodynamic quantities associated with the phase transitions were compared among the lipid bilayers. For all the bilayers studied, the pressure-induced interdigitated gel (L beta I) phase appeared above the critical interdigitation pressure (CIP) between the L beta' and P beta' phases. The CIPs for the PSPC, C17PC and SPPC bilayers were found to be 50.6, 79.1 and 93.0 MPa, respectively. Contribution of two acyl chains to thermodynamic properties for the phase transitions of asymmetric PSPC and SPPC bilayers was not even. The sn-2 acyl chain lengths of asymmetric PCs governed primarily the bilayer properties. The fluorescence spectra of Prodan in lipid bilayers showed the emission maxima characteristic of bilayer phases, which were dependent on the location of Prodan in the bilayers. Second derivative of fluorescent spectrum exhibited the original emission spectrum of Prodan to be composed of the distribution of Prodan into multiple locations in the lipid bilayer. The F''497/F''430 value, a ratio of second derivative of fluorescence intensity at 497 nm to that at 430 nm, is decisive evidence whether bilayer interdigitation will occur. With respect to the L beta'/L beta I phase transition in the SPPC bilayer, the emission maximum of Prodan exhibited the narrow-range red-shift from 441 to 449 nm, indicating that the L beta I phase in the SPPC bilayer has a less polar "pocket" formed by a space between uneven terminal methyl ends of the sn-1 and sn-2 chains, in which the Prodan molecule remains stably. (Keyword) 2-Naphthylamine / Calorimetry, Differential Scanning / Hydrostatic Pressure / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Phospholipids / Spectrometry, Fluorescence / Temperature / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbamem.2007.12.009 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18190778 ● Search Scopus @ Elsevier (PMID): 18190778 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2007.12.009 (DOI: 10.1016/j.bbamem.2007.12.009, PubMed: 18190778)
49.	Masataka Kusube, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Bilayer Phase Transitions of N-methylated Dioleoylphosphatidylethanolamines under High Pressure, Chemistry and Physics of Lipids, Vol.142, No.1-2, 94-102, 2006. (Summary) The bilayer phase transitions of four kinds of unsaturated phospholipids with different-sized polar head groups, dioleoylphosphatidylethanolamine (DOPE), dioleoylphosphatidyl-N-methylethanolamine (DOMePE), dioleoylphosphatidyl-N,N-dimethylethanolamine (DOMe2PE) and dioleoylphosphatidylcholine (DOPC), were observed by means of differential scanning calorimetry (DSC) and high-pressure light-transmittance. DSC thermogram and light-transmittance curve for each phospholipid vesicle solution exhibited only one phase transition under ambient pressure, respectively. The light-transmittance of DOPC solution at pressure higher than 234 MPa abruptly increased stepwise at two temperatures, which corresponds to the appearance of stable subgel and lamellar gel phases under high pressure in addition to the liquid crystal phase. The constructed temperature (T)-pressure (p) phase diagrams were compared among these phospholipids. The phase-transition temperatures of the phospholipids decreased stepwise by N-methylation of the head group. The slops of the T-p phase boundary (dT/dp) of DOPE, DOMePE and DOMe2PE bilayers (0.127-0.145 K MPa-1) were found to be close to that of the transition from the lamellar crystal (or subgel; Lc) phase to the liquid crystal (Lalpha) phase for DOPC bilayer (0.131 K MPa-1). On the other hand, the dT/dp value of the main transition from the lamellar gel (Lbeta) phase to the Lalpha phase for DOPC bilayer (0.233 K MPa-1) was significantly different from that of the Lc/Lalpha transition, hence both curves intersected with each other at 234 MPa. The thermodynamic quantities associated with the phase transition of DOPE, DOMePE and DOMe2PE bilayers had also similar values to those for the Lc/Lalpha transition of DOPC bilayer. Taking into account of the values of transition temperature, dT/dp and thermodynamic quantities compared with the corresponding results of saturated phospholipids, we identified the phase transitions observed in the DOPE, DOMePE and DOMe2PE bilayers as the transition from the Lc phase to the Lalpha phase although they have been regarded as the main transition in the previous studies. The Lbeta phase is probably unstable for DOPE, DOMePE and DOMe2PE bilayers at all pressures, it exists as a metastable phase at pressures below 234 MPa while as a stable phase at pressures above 234 MPa in DOPC bilayer. The difference in phase stability among the phospholipid bilayers is originated from that in hydration structure of the polar head groups. (Keyword) Colorimetry / Lipid Bilayers / Methylation / Phase Transition / Phosphatidylcholines / Phosphatidylethanolamines / Stress, Mechanical / Thermodynamics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.chemphyslip.2006.03.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16620796 ● Search Scopus @ Elsevier (PMID): 16620796 ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2006.03.004 (DOI: 10.1016/j.chemphyslip.2006.03.004, PubMed: 16620796)
50.	Ryosuke Sueyoshi, Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Barotropic phase transition between the lamellar liquid crystal phase and the inverted hexagonal phase of dioleoylphosphatidylethanolamine, Colloids and Surfaces B:Biointerfaces, Vol.50, No.1, 85-88, 2006. (Summary) The phase transition between the lamellar liquid crystal (Lalpha) phase and the inverted hexagonal (H(II)) phase of dioleoylphosphatidylethanolamine (DOPE) in aqueous NaCl solutions was observed by means of differential scanning calorimetry (DSC) under ambient pressure and light-transmittance technique under high pressure. The pressure dependence of the transition temperature (dT/dp) and the thermodynamic quantities for the Lalpha/H(II) transition were compared with those of another phase transition found in the DOPE bilayer membrane, which is the transition from the lamellar crystal (Lc) phase to the Lalpha phase. The dT/dp value of the Lalpha/H(II) transition was about 3.5 times as large as that of the Lc/Lalpha transition while the thermodynamic quantities were significantly smaller than those of the latter to the contrary. Comparing the enthalpy and volume behavior of the Lalpha/H(II) transition with that of the Lc/Lalpha transition, we concluded that the Lalpha/H(II) transition can be regarded as the volume-controlled transition for the reconstruction of molecular packing. (Keyword) Calorimetry, Differential Scanning / Crystallization / Lipid Bilayers / Liquid Crystals / Models, Biological / Phase Transition / Phosphatidylethanolamines / Sodium Chloride / Solutions / Thermodynamics / Water (Link to Publication Site) ● Publication site (DOI): 10.1016/j.colsurfb.2006.04.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16697154 ● Search Scopus @ Elsevier (PMID): 16697154 ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2006.04.001 (DOI: 10.1016/j.colsurfb.2006.04.001, PubMed: 16697154)
51.	Hitoshi Matsuki, Masaki GOTO, Masataka Kusube, Nobutake Tamai and Shoji Kaneshina : Barotropic Phase Transitions of 1-Palmitoyl-2-stearoylphosphatidylcholine Bilayer Membrane, Chemistry Letters, Vol.34, No.2, 270-271, 2005. (Summary) 非対称型リン脂質1-パルミトイル-2-ステアロイルホスファチジルコリン二分子膜の圧力誘起相転移を高圧光透過率測定および示差走査熱量測定により観測した．決定した相転移点に基づいて温度—圧力相図を構築し，3種類の対称型ホスファチジルコリン二分子膜の相図と比較した．1-パルミトイル-2-ステアロイルホスファチジルコリン二分子膜の相挙動は対称型ホスファチジルコリン二分子膜とは大きく異なり，指組み構造形成の臨界圧力は全ての脂質のうちで最も小さくなった． (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.2005.270 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1574231874639495424 ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2005.270 (DOI: 10.1246/cl.2005.270, CiNii: 1574231874639495424)

Academic Paper (Unrefereed Paper):

1.	Masaki GOTO : Visiting Research Fellow at ETH, Paul Scherrer Institute, The Review of High Pressure Science and Technology, Vol.25, No.3, 251-252, 2015. (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.25.251 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84941766485 (DOI: 10.4131/jshpreview.25.251, Elsevier: Scopus)
2.	Hitoshi Matsuki, Masaki GOTO, Nobutake Tamai and Mikito Yasuzawa : :, Bulletin of Institute of Technology and Science, The University of Tokushima, Vol.55, 25-30, 2010. (Summary) Soft nanotechnology is technology that treats soft and wet materials such as self-assemblysystems in a living body under a relatively moderate condition. We focus our attention on the sizecontrol of liposomes, namely lipid bilayer membranes and performed the soft nanotechnology insizing of liposomes under high pressure. Two high-pressure sizing techniques, a method ofcontinuous pressure relaxation using a phase transition between gel and liquid crystalline phases ofthe bilayer membrane and that of a pressure-induced gel phase using a phase transition betweenbilayer and nonbilayer membranes, were applied to liposomes of distearoylphosphatidylcholine, andthe effects of the both methods were considered. Further, the application of giant liposomescontrolled by high-pressure sizing to microsensor was briefly described. (Tokushima University Institutional Repository) ● Metadata: 66693 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050001337463705088 (Tokushima University Institutional Repository: 66693, CiNii: 1050001337463705088)

Review, Commentary:

1.	Masaki GOTO : Study on phase transition of lipid nano particles under high pressure, MEMBRANE, Vol.48, No.4, 163-167, Jul. 2023. (Keyword) lipid bilayer membrane / Phospholipid / high pressure
2.	Masaki GOTO : Effect of Hydrophobic Chain Linkage Mode on Lipid Bilayer Phase Behavior: A Study Using Unnatural Amide-Linked Phospholipids, Colloid and Interface Communication, Vol.47, No.3, 18-21, Aug. 2022.
3.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Membrane States of Saturated Glycerophospholipids: A Thermodynamic Study of Bilayer Phase Transitions, Chemical & Pharmaceutical Bulletin, Vol.67, No.4, 300-307, Apr. 2019. (Keyword) bilayer membrane / phase transition / glycerophospholipid / high pressure (Link to Publication Site) ● Publication site (DOI): 10.1248/cpb.c18-00954 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30930432 ● Summary page in Scopus @ Elsevier: 2-s2.0-85064011553 (DOI: 10.1248/cpb.c18-00954, PubMed: 30930432, Elsevier: Scopus)
4.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Pressure Perturbation Calorimetry: Application to Lipid Membranes, Netsu Sokutei, Vol.46, No.2, 76-80, Apr. 2019. (Summary) Pressure perturbation calorimetry (PPC) is a novel calorimetric technique which allows us to obtain information about volumetric properties of solute molecules and colloidal particles in aqueous solutions. A PPC calorimeter has basically the same architecture as a high-sensitivity power-compensation differential scanning calorimeter, but it can measure the heat quantity transferred from or into a sample solution during the course of an isothermal small pressure change by attaching an external accessary for pressure control to the calorimeter. This heat quantity can be related to the expansion coefficient α, and thus, the temperature dependence of α can be obtained by performing PPC scans at a series of different temperatures. This article describes the application of PPC to the investigation of volume behavior of phospholipid bilayer membranes on the basis of our previous studies along with the general thermodynamic background of the PPC technique. We also give a concise explanation of how to analyze the PPC data, because we modified the conventional data analysis method to evaluate the absolute values of volume changes with bilayer phase transitions. (Keyword) pressure perturbation calorimetry / lipid membrane / volume behavior / apparent molar volume / thermal expansivity (Link to Publication Site) ● Publication site (DOI): 10.11311/jscta.46.2_76 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520290882709222784 ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.46.2_76 (DOI: 10.11311/jscta.46.2_76, CiNii: 1520290882709222784)
5.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : ホスファチジルエタノールアミン二重膜の温度および圧力誘起相転移, MEMBRANE, Vol.44, No.2, 40-49, Mar. 2019. (Link to Publication Site) ● Publication site (DOI): 10.5360/membrane.44.40 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.5360/membrane.44.40 (DOI: 10.5360/membrane.44.40)
6.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Association Behavior of Double Chain Ionic Surfactants: Elucidation of the Membrane States by High-pressure Study, The Review of High Pressure Science and Technology, Vol.28, No.2, 81-87, Jul. 2018. (Summary) The bilayer phase transitions of double-chain cationic surfactants, dialkyldimethylammonium bromides (2CnBr), at ambient and high pressures were investigated. The 2CnBr bilayers underwent a single or multiple phase transitions depending on the alkyl-chain length. Comparing the phase-transition properties among the 2CnBr bilayers, the temperature pressure phase diagrams and thermodynamic quantities for the phase transitions were determined. We could comprehensively interpret the complicated phase behavior of the 2CnBr bilayers, which has not been revealed by conventional experimental methods at ambient pressure. (Keyword) Double chain cationic surfactant / bilayer membrane / phase transition / high pressure (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.28.81 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85050766308 (DOI: 10.4131/jshpreview.28.81, Elsevier: Scopus)
7.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : コレステロールが誘起する脂質膜側方相分離の熱力学的考察, Netsu Sokutei, Vol.44, No.3, 101-107, Jul. 2017. (Link to Publication Site) ● Publication site (DOI): 10.11311/jscta.44.3_101 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.44.3_101 (DOI: 10.11311/jscta.44.3_101)
8.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Quantitative Observation of Volume and Relaxation Behavior of Phospholipid Bilayers by Pressure Perturbation Calorimetry, The Review of High Pressure Science and Technology, Vol.25, No.2, 109-115, May 2015. (Summary) Pressure perturbation calorimetry (PPC) is a relatively new calorimetric technique which allows us to investigate volume behavior of solute molecules in aqueous solutions or colloidal particles in aqueous dispersions. This article deals with the application of the PPC technique to phospholipid vesicle dispersions along with a brief description about the theoretical aspect of this technique on the basis of our recent studies on the volume and relaxation behavior of phospholipid bilayers. (Keyword) bilayer / isothermal compressibility / partial molar volume / phospholipid / pressure perturbation calorimetry / relaxation behavior / thermal expansivity (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.25.109 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204380727040 ● Summary page in Scopus @ Elsevier: 2-s2.0-84931074285 (DOI: 10.4131/jshpreview.25.109, CiNii: 1390001204380727040, Elsevier: Scopus)
9.	Masaki GOTO : ハロゲン化ジアルキルジメチルアンモニウム二重膜の会合挙動:高圧力研究による相状態の解明, C & I commun colloid & interface communication : newsletter from DCSC, Vol.1, No.40, 31-33, Mar. 2015.
10.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 脂質ラフトは熱力学的に説明できるのか?―非調和融点型固液相図とクラスター形成―, Seibutsu Butsuri, Vol.54, No.3, 154-157, May 2014. (Link to Publication Site) ● Publication site (DOI): 10.2142/biophys.54.154 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.2142/biophys.54.154 (DOI: 10.2142/biophys.54.154)
11.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 飽和ジアシルホスファチジルコリン二重膜の温度および圧力誘起相転移, Netsu Sokutei, Vol.41, No.2, 66-73, Apr. 2014. (Link to Publication Site) ● Publication site (DOI): 10.11311/jscta.41.2_66 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.41.2_66 (DOI: 10.11311/jscta.41.2_66)
12.	Masaki GOTO : Imaging of Phosphatidylcholine Bilayers by a High-Pressure Fluorometry, The Review of High Pressure Science and Technology, Vol.23, No.1, 157-166, May 2013. (Summary) High-pressure fluorescence study using a polarity-sensitive probe Prodan on phosphatidylcholine (PC) bilayers is reviewed. The author has constructed a three-dimensional image plot based on the second-derivative of the Prodan fluorescence spectra that change depending on the phase state of the PC bilayers, which enables us to examine the packing state of the bilayer. The effects of (1) acyl chain length, (2) vesicle size, and (3) chain asymmetry on the PC bilayers are discussed by using the technique of bilayer imaging. Further the formation of the lamellar crystal phase of the PC bilayer is also revealed by the technique. (Keyword) Bilayer membrane / Bilayer imaging / Packing state / Phosphatidylcholine / Phase transition / Prodan fluorescence (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.23.157 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679358404224 ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.23.157 (DOI: 10.4131/jshpreview.23.157, CiNii: 1390282679358404224)
13.	Hitoshi Matsuki and Masaki GOTO : Control of Lipid Phase Transitions by Applying High Pressure, 冷凍, Vol.88, No.1026, 274-279, Apr. 2013. (Keyword) High pressure / Lipid bilayer membrane / Phase stability / Phase transition / Neutral fat / Non-bilayer structure (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520009408125051776 (CiNii: 1520009408125051776)
14.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Membrane States of Lipids in Biological Membranes -Structure-Function Relationship Revealed from Pressure Study-, The Review of High Pressure Science and Technology, Vol.23, No.1, 30-38, Feb. 2013. (Summary) Bilayer-membrane properties of the most frequently used lipid in membrane studies, dipalmitoylphosphatidylcholine (DPPC), are described. And then, barotropic bilayer phase behavior of various kinds of phospholipids with a different molecular structure from DPPC is explained in comparison with that of the DPPC bilayer. A slight difference in the molecular structure has a marked influence on the membrane state of the bilayer. Systematic pressure study using the DPPC bilayer enables us to understand the role and meaning of various phospholipids existing in biological membranes. (Keyword) bilayer membrane / dipalmitoylphosphatidylcholine / phase stability / phase transition / phospholipids / pressure (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.23.30 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204380540672 ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.23.30 (DOI: 10.4131/jshpreview.23.30, CiNii: 1390001204380540672)
15.	Hitoshi Matsuki, Masaki GOTO, Kaori Tada and Nobutake Tamai : Thermotropic and Barotropic Phase Behavior of Phosphatidylcholine Bilayers, --- Phospholipids: Molecular Sciences 2012 ---, International Journal of Molecular Sciences, Vol.14, No.2, 2282-2302, Jan. 2013. (Link to Publication Site) ● Publication site (DOI): 10.3390/ijms14022282 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84875095682 (DOI: 10.3390/ijms14022282, Elsevier: Scopus)
16.	Hitoshi Matsuki, 多田佳織, Masaki GOTO and Nobutake Tamai : Barotropic Phase Transitions of Asymmetric Phospholipid Bilayer Membranes : Molecular Asymmetry and Phase Stability, The Review of High Pressure Science and Technology, Vol.20, No.4, 306-314, Nov. 2010. (Summary) The effect of pressure on the bilayer phase transitions of two kinds of asymmetric phospholipids, phosphatidylcholines (PCs) with different saturated acyl chains in the sn-1 and sn-2 positions on the glycerol backbone and mixed-chain PCs with an unsaturated acyl chain in the sn-1 or sn-2 position, is described. The alignment of the sn-1 and sn-2 acyl chains at the therminal methyl ends and the mismatch of the effective chain length between the sn-1 and sn-2 acyl chains produce a significant influence on their bilayer properties. The correlation between molecular asymmetry of PC molecules and phase stabilities of the bilayer membranes is discussed. (Keyword) asymmetric phospholipid / bilayer membrane / phase stability / phase transition / pressure / symmetric phospholipid (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.20.306 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204381315968 ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.20.306 (DOI: 10.4131/jshpreview.20.306, CiNii: 1390001204381315968)
17.	Masaki GOTO : High-Pressure Fluorescence Study on Bilayer Phase Behavior of Symmetric and Asymmetric Phospholipids, The Review of High Pressure Science and Technology, Vol.18, No.3, 267-272, Aug. 2008. (Summary) High-pressure fluorescence study on the bilayer phase behavior of symmetric and asymmetric phospholipids in my doctoral thesis is briefly reviewed. It has been clarified by the novel analysis of the second derivatives of fluorescent spectra that the fluorescent probe Prodan resides multiple sites in the bilayer membrane of phosphatidylcholines (PCs) depending on the membrane states. Comparing the diagrams of three dimensional fluorescent spectra among them, the present results revealed that the slight difference in acyl chain length between the sn-1 and sn-2 positions in the PC molecules provides significant influence on their bilayer phase behavior, especially in the bilayer interdigitation. (Keyword) asymmetric phospholipid / bilayer membrane / phase transition / fluorescence / pressures-induced interdigitation / prodan fluorescence (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.18.267 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204381120000 ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.18.267 (DOI: 10.4131/jshpreview.18.267, CiNii: 1390001204381120000)

Proceeding of International Conference:

1.	Kana Kajiura, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Phase Transitions of Acidic Phospholipid Bilayer Membranes: Effect of Charged Head-Group Size, 26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023.
2.	Nobutake Tamai, Mei Kamiya, Masaki GOTO and Hitoshi Matsuki : Bilayer Phase Transitions of Phospholipid in Aqueous Solutions of Monosaccharides, 26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023.
3.	Masaki GOTO, Ayaka Mukae, Shuntaro Yoshida, Nobutake Tamai and Hitoshi Matsuki : Pressure-Induced Bilayer Interdigitation of Lipid Membranes: Characteristics and Formation Mechanism, 26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023.
4.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Bilayer Phase Imaging by Prodan Fluorescence Spectroscopy, 13th International Congress on Membranes and Membrane Processes (ICOM2023), Chiba, Jul. 2023.
5.	Masaki GOTO, Ayaka Mukae, Nobutake Tamai and Hitoshi Matsuki : Pressure-Induced Interdigitation of Lipid Membranes: Difference from Chemically Induced Interdigitation and the Formation Mechanism, 13th International Congress on Membranes and Membrane Processes (ICOM2023), Chiba, Jul. 2023.
6.	Nobutake Tamai, Nono Kiriyama, Masaki GOTO, Hitoshi Matsuki and Kazuhiro Fukada : Thermodynamic Study on the Effect of Monosaccharides on Phase Transitions of Phospholipid Bilayer Membrane, The 8th International Symposium of International Society of Rare Sugars (RSC2023), Takamatsu, Apr. 2023.
7.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Thermodynamic Study on Bilayer Phase Transitions of Twin-Tailed Cationic Surfactants, The 5th International Kyushu Colloid Colloquium, Okinawa, Nov. 2019.
8.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : High-Pressure Fluorometric Study on Bilayer Packing of Phosphatidylcholines, Okinawa Colloid 2019, Okinawa, Nov. 2019.
9.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Suppression of the Formation of Intgerdigitated Structure in Ether-Linked Phosphatidylcholine Bilayer by Cholesterol, Okinawa Colloid 2019, Okinawa, Nov. 2019.
10.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Membrane Fusion of Phospholipid Bilayers Induced by High Pressure, Okinawa Colloid 2019, Okinawa, Nov. 2019.
11.	Masaki GOTO, Shuntaro Yoshida, Nobutake Tamai and Hitoshi Matsuki : Thermodynamic Study on Ligand Partitioning into Lipid Bilayer Membranes under High Pressure, 10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018.
12.	Shuntaro Yoshida, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Prodan Is a Useful Packing Indicator in Lipid Membranes, Which Discerns Various Kinds of Phase Transitions, 10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018.
13.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Pressure-Induced Membrane Fusion of Phospholipid Bilayers: Irreversible and Spherical Growth of Giant Unilamellar Vesicles, 10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018.
14.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Thermotropic Phase Behavior of Sterol-Containing Binary Bilayer Membranes of Diacylphosphatidylcholines, 8th International and 10th Japan-China Joint Symposium on Calorimetry and Thermal Analysis (CATS-2017), Fukuoka, Nov. 2017.
15.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : Pressure Perturbation Calorimetry for the Volume Characterization of Lipid Bilayers, 4th Joint Meeting of the 71st Calorimetry Conference (CALCON2016) and the Japan Society of Calorimetry and Thermal Analysis (JSCTA), Turtle Bay, Oahu, Hawaii, Aug. 2016.
16.	Hitoshi Matsuki, Shigeru Endo, Ryosuke Sueyoshi, Masaki GOTO and Nobutake Tamai : Temperature- and Pressure-Induced Phase Transitions of Diacylphosphatidylethanolamine Bilayers, 4th Joint Meeting of the 71st Calorimetry Conference (CALCON2016) and the Japan Society of Calorimetry and Thermal Analysis (JSCTA), Turtle Bay, Oahu, Hawaii, Aug. 2016.
17.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Effect of Pressure on Bilayer Phase Behavior of N-methylated Di-O-hexadecylphosphatidylethanolamines, 9th International Conference on High Pressure Bioscience and Biotechnology (HPBB2016), Toronto, Jul. 2016.
18.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Effect of Hydrophobic Chain Structure on Phase Transitions of Diacylphosphatidylethanolamine Bilayers under High Pressure, 9th International Conference on High Pressure Bioscience and Biotechnology (HPBB2016), Toronto, Jul. 2016.
19.	Takahiro Yano, Yoshioka Tatsuro, Nobutake Tamai, Masaki GOTO, Hitoshi Matsuki, Nagasawa Makoto and Matsumoto Masamitsu : Precise Measuring System of Density for Liquids under High Pressure, 8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014.
20.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Barotropic Structural Change of Lipid Membranes: Characteristics and Mechanism of Bilayer Interdigitation, 8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014.
21.	Takahiro Yano, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Barotropic Phase Behavior of Asymmetric Phosphatidylcholine Bilayers, 8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014.
22.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : Pressure-induced Bilayer Interdigitation: Model of Molecular-ordering Formation in Lipid Membranes, The 2nd International Symposium ``Dynamical ordering of biomolecular systems for creation of integrated functions'', Kyoto, Jan. 2014.
23.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : High-pressure fluorometric study on the subgel-formation of phosphatidylcholine bilayer membrane, 7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012.
24.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : An attempt to determine the viscoelastic properties of phospholipid bilayer membranes, 7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012.
25.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : How do membranes response by applying high hydrostatic pressure?, 7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012.
26.	Nobutake Tamai, Sanae Inazawa, Daiki Fijiwara, Masaki GOTO, Shoji Kaneshina and Hitoshi Matsuki : Effect of Stigmasterol and Ergosterol on the Thermotropic Bilayer Phase Behavior of Dipalmitoylphosphatidylcholine, 14th International Association of Colloid and Interface Scientists, Conference (IACIS2012), Sendai, May 2012.
27.	Hitoshi Matsuki, Saeko Tanaka, Masaki GOTO and Nobutake Tamai : Barotropic Bilayer Phase Behavior of an Acidic Phospholipid, Dipalmitoylphosphatidylglycerol, 14th International Association of Colloid and Interface Scientists, Conference (IACIS2012), Sendai, May 2012.
28.	Saeko Tanaka, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer Membrane in Saline Water under High Pressure, 6th International Conference on Advanced Materials Development and Performance (AMDP2011), Tokushima, Jul. 2011.
29.	Nobutake Tamai, Maiko Tsutsui, Masaki GOTO and Hitoshi Matsuki : Investigation on Volumetric Properties of Phospholipid Aggregates by Pressure Perturbation Calorimetry, 6th International Conference on Advanced Materials Development and Performance (AMDP2011), Tokushima, Jul. 2011.
30.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : High-pressure fluorescence study on packing states of phosphatidylcholine bilayers, The 6th meeting of the Study of Matter at Extreme Conditions (SMEC2011), Miami, Apr. 2011.
31.	Sanae Inazawa, Takuya Izumikawa, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Comparison between effects of cholesterol and stigmasterol on bilayer phase behavior of dipalmitoylphosphatidylcholine, The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010.
32.	Maiko Tsutsui, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Themotropic volume behavior of bilayer membranes of saturated and unsaturated phosphatidylcholines, The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010.
33.	Nobutake Tamai, Takuya Izumikawa, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Thermotropic phase behavior of binary bilayer membranes of cholesterol and a homologous series of saturated diasylphosphatidylcholines of different chain lengths, The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010.
34.	Hitoshi Matsuki, Agnieszka Broniek, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Phase behavior of plasmalogen bilayer membrane under high pressure, The 3rd International Kyushu Colloid Colloquium, Fukuoka, Sep. 2010.
35.	Hitoshi Matsuki, Hiroshi Sawaguchi, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Subgel Phase Formation on Bilayer Membranes of Diacyl- and Dialkylphosphatidylcholines with Varying Acyl Chain Lengths, International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010.
36.	Saeko Tanaka, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer under High Pressure, International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010.
37.	Ryo Kishizoe, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Anomalous Phase Behavior of Ditridecanoylphosphatidylcholine Bilayer under High Pressure, International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010.
38.	Sanae Inazawa, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Comparative Study on Effects of Stigmasterol and Cholesterol on Bilayer Phase Behavior of Dipalmitoylphosphatidylcholine, International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010.
39.	Nobutake Tamai, Takuya Izumikawa, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Acyl-chain Length Dependent and Independent Effects of Chlolesterol on Bilayer Phase Behavior of Diacylphosphatidylcholines, International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010.
40.	Nobutake Tamai, Yuko Nambu, Maiko Tsutsui, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : Volume properties of diacylphosphatidylcholine bilayer membranes revealed by pressure perturbation calorimetry and densitometry, 6th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2010), Freising, Germany, Aug. 2010.
41.	Hitoshi Matsuki, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Packing state in bilayer membranes of diacylphosphatidylcholines with varying acyl chain lengths under high pressure, 6th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2010), Freising, Germany, Aug. 2010.
42.	Nobutake Tamai, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina : A mechanism of pressure-induced interdigitation of lipid bilayers, Journal of Physics: Conference Series, Vol.215, No.1, 12161, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012161 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012161 (DOI: 10.1088/1742-6596/215/1/012161)
43.	Takuya Izumikawa, Yuko Nambu, Kazuyo Fujishige, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Volume behaviour of dipalmitoylphosphatidylcholine bilayer membrane: pressure perturbation calorimetry and densitometry, Journal of Physics: Conference Series, Vol.215, No.1, 12163, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012163 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012163 (DOI: 10.1088/1742-6596/215/1/012163)
44.	Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Effect of pressure on the bilayer phase transitions of asymmetric lipids with an unsaturated acyl chain in sn-1 position, Journal of Physics: Conference Series, Vol.215, No.1, 12162, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012162 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012162 (DOI: 10.1088/1742-6596/215/1/012162)
45.	Makoto Nishimoto, Masaki GOTO, Nobutake Tamai, Hideaki Nagamune, Shoji Kaneshina and Hitoshi Matsuki : Effect of pressure on interactions of anti-fluorescent probe monoclonal antibody with a ligand and inhibitors, Journal of Physics: Conference Series, Vol.215, No.1, 12157, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012157 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012157 (DOI: 10.1088/1742-6596/215/1/012157)
46.	Hitoshi Matsuki, Makoto Nishimoto, Kaori Tada, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina : Thermodynamic characterization of bilayer-nonbilayer phase transitions of phospholipid membranes, Journal of Physics: Conference Series, Vol.215, No.1, 12160, Bristol, Mar. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012160 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012160 (DOI: 10.1088/1742-6596/215/1/012160)
47.	Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Bilayer Phase Behavior of an Asymmetric Lipid, 1-Stearoyl-2-palmitoylphosphatidylcholine, under High Pressure, The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 73-78, Tsukuba, Aug. 2007.
48.	Kaori Tada, Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Barotropic Phase Transitions of Dilauroylphosphatidylcholine Bilayer Membrane, The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 68-72, Tsukuba, Aug. 2007.
49.	Masataka Kusube, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : Effect of Pressure on the Prodan Fluorescence in Bilayer Membrane of Ether-linked Lipid, Dihexadecylphosphatidylcholine, The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 61-67, Tsukuba, Aug. 2007.

Proceeding of Domestic Conference:

1.	Masaki GOTO, 松下里李夏, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜は光学活性リガンドを認識できるのか?, 2023年度麻酔メカニズム研究会, Dec. 2023.
2.	角田芙美, Nobutake Tamai, Masaki GOTO, Mikito Yasuzawa and Hitoshi Matsuki : 極性頭部荷電が異なるコリンホスフェート型脂質の有機合成, 2023年度日本化学会中国四国支部大会, Nov. 2023.
3.	角田芙美, Nobutake Tamai, Masaki GOTO, Mikito Yasuzawa and Hitoshi Matsuki : 荷電状態の異なる極性頭部転置型リン脂質の有機合成, 第37回九州コロイドコロキウム, Nov. 2023.
4.	中尾俊樹, Masaki GOTO, Hitoshi Matsuki and 加藤稔 : FTIR法によるラセミ体アミド結合型リン脂質の温度・圧力誘起相転移, 第64回高圧討論会, Nov. 2023.
5.	榎本賢太郎, 尾形茉実, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧力下におけるハロゲン化ジアルキルジメチルアンモニウム二重膜の相挙動, 第64回高圧討論会, Nov. 2023.
6.	羽原誉幸, 川原宏介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 不飽和リン脂質混合二重膜の圧力誘起膜融合, 第64回高圧討論会, Nov. 2023.
7.	松岡颯大, 梶浦可菜, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧下における酸性リン脂質ジパルミトイルホスファチジン酸二重膜の相挙動, 第64回高圧討論会, Nov. 2023.
8.	Masaki GOTO, 松岡楓太, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質ジミリストイルホスファチジルグリセロール二重膜の圧力誘起相転移, 第64回高圧討論会, Nov. 2023.
9.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 脂質膜研究の新展開:系統脂質学から化学脂質学へ, 第64回高圧討論会, Nov. 2023.
10.	Nobutake Tamai, 桐山野乃, 神谷芽生, Masaki GOTO, 深田和宏 and Hitoshi Matsuki : 化学構造の異なる四種類のアルドおよびケトヘキソースがリン脂質二重膜の相転移におよぼす影響, 第59回熱測定討論会, Oct. 2023.
11.	梶浦可菜, 松岡颯大, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 極性頭部の有無が酸性リン脂質の二重膜相転移におよぼす影響:特異的な添加塩濃度依存性, 第74回コロイドおよび界面化学討論会, Sep. 2023.
12.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジアルキルホスファチジルコリン二重膜のサブゲル相形成, 第74回コロイドおよび界面化学討論会, Sep. 2023.
13.	角田芙美, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 配列変異非天然脂質の有機合成, 第14回日本生物物理学会中国四国支部大会, May 2023.
14.	Nobutake Tamai, 角田芙美, Masaki GOTO and Hitoshi Matsuki : 系統脂質学から化学脂質学へ:新たな脂質膜研究の展開, 第14回日本生物物理学会中国四国支部大会, May 2023.
15.	Masaki GOTO, 中尾俊樹, Nobutake Tamai and Hitoshi Matsuki : ラセミ体アミド結合型長鎖ホスファチジルコリンの圧力誘起二重膜相転移, 第63回高圧討論会, Dec. 2022.
16.	梶浦可菜, 松岡颯大, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜の圧力誘起相転移 -酸性リン脂質ジパルミトイルホスファチジン酸-, 第63回高圧討論会, Dec. 2022.
17.	関谷朋美, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 光散乱法によるリン脂質ベシクルの形態観察, 2022年度日本化学会中国四国支部大会, Nov. 2022.
18.	山崎名津美, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 極性頭部転置ベタイン型脂質の有機合成と二重膜物性, 2022年度日本化学会中国四国支部大会, Nov. 2022.
19.	Nobutake Tamai, 横矢祐香, Masaki GOTO and Hitoshi Matsuki : アルキル鎖長の異なる1:1-カチオンーアニオン界面活性剤イオン対の水中における会合体形成, 第58回熱測定討論会, Oct. 2022.
20.	Toshiki Nakao, Masaki GOTO, Nobutake Tamai, Minoru Kato and Hitoshi Matsuki : Bilayer Properties of a Glycero- and Sphingo-Mixed Type Phospholipid, The 73rd Divisional Meeting of Division of Colloid and Surface Chemistry, The Chemical Society of Japan, Sep. 2022.
21.	Nobutake Tamai, 横矢祐香, Masaki GOTO and Hitoshi Matsuki : 水中での非対称型カチオン-アニオン界面活性剤の会合体形成, 第73回コロイドおよび界面化学討論会, Sep. 2022.
22.	Masaki GOTO, 中尾俊樹, Nobutake Tamai and Hitoshi Matsuki : 高圧下におけるラセミ体アミド結合型長鎖ホスファチジルコリン二重膜の相挙動, 第73回コロイドおよび界面化学討論会, Sep. 2022.
23.	桐山野乃, 神谷芽生, Nobutake Tamai, Masaki GOTO, 深田和宏 and Hitoshi Matsuki : 単糖水溶液中でのリン脂質二分子膜のゲル-液晶相転移, 第36回九州コロイドコロキウム, Aug. 2022.
24.	松岡楓太, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧下におけるジミリストイルホスファチジルグリセロール二重膜の相挙動, 第36回九州コロイドコロキウム, Aug. 2022.
25.	梶浦可菜, 成瀬由希, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質ホスファチジン酸の二重膜相転移:荷電頭部サイズの効果, 第36回九州コロイドコロキウム, Aug. 2022.
26.	梶浦可菜, 成瀬由希, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質二重膜の相転移:荷電頭部サイズが膜状態におよぼす影響, 日本膜学会第44年会, Jun. 2022.
27.	桐山野乃, 神谷芽生, Nobutake Tamai, Masaki GOTO, 深田和宏 and Hitoshi Matsuki : リン脂質二重膜のゲル–液晶相転移におよぼす単糖の効果, 日本膜学会第44年会, Jun. 2022.
28.	松岡楓太, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジミリストイルホスファチジルグリセロール二重膜の圧力誘起相転移, 第13回日本生物物理学会中国四国支部大会, May 2022.
29.	名田智哉, 尾形茉実, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ヨウ化ジテトラデシルジメチルアンモニウム二重膜の相挙動, 第13回日本生物物理学会中国四国支部大会, May 2022.
30.	迎綾香, 下田毬絵, 金岡大樹, 新居佳孝, 山﨑博子, Keizo Yuasa, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 柑橘類果皮抽出液による真皮線維芽細胞コラーゲン産生作用, 第13回日本生物物理学会中国四国支部大会, May 2022.
31.	大西沙弥, 西谷真美, 下野萌香, 下田毬絵, 金岡大樹, 山﨑博子, Keizo Yuasa, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : スダチ果皮抽出液による表皮アクアポリン3の発現制御機構の解明, 第13回日本生物物理学会中国四国支部大会, May 2022.
32.	角田芙美, 中尾俊樹, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 光学活性アミド結合型リン脂質の有機合成と二重膜特性, 第13回日本生物物理学会中国四国支部大会, May 2022.
33.	中尾俊樹, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : ホスファチジルコリン二重膜におよぼす疎水鎖結合様式の影響, 第59回生物物理学会年会, Nov. 2021.
34.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : エーテル結合型ホスファチジルコリン膜のサブゲル相形成, 膜シンポジウム2021, Nov. 2021.
35.	Nobutake Tamai, 清水建翔, Masaki GOTO and Hitoshi Matsuki : アルキル鎖長の等しい1:1-カチオン-アニオン界面活性剤イオン対の水中における会合体形成, 第57回熱測定討論会, Oct. 2021.
36.	Masaki GOTO, 中尾俊樹, Nobutake Tamai and Hitoshi Matsuki : 高圧下におけるアミド結合型リン脂質ジアシルアミドデオキシホスファチジルコリン二重膜の相挙動, 第62回高圧討論会, Oct. 2021.
37.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : ホスファチジルコリン二重膜のサブゲル相形成, 第62回高圧討論会, Oct. 2021.
38.	松下里李夏, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 光学活性リン脂質二重膜の相転移とリガンド認識, 第35回九州コロイドコロキウム, Sep. 2021.
39.	中尾俊樹, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : グリセロ-スフィンゴ混合型非天然リン脂質の二分子膜相転移, 第35回九州コロイドコロキウム, Sep. 2021.
40.	Masaki GOTO, 中尾俊樹, Nobutake Tamai and Hitoshi Matsuki : アミド結合型リン脂質ジアシルアミドデオキシホスファチジルコリン二重膜の常圧および高圧相転移, 第72回コロイドおよび界面化学討論会, Sep. 2021.
41.	Nobutake Tamai, 清水建翔, Masaki GOTO and Hitoshi Matsuki : 水中での対称型カチオン-アニオン界面活性剤の会合体形成, 第72回コロイドおよび界面化学討論会, Sep. 2021.
42.	長尾苑, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 光散乱法によるリン脂質ベシクルの構造安定性評価, 日本膜学会第43年会, Jun. 2021.
43.	中尾俊樹, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : モジュール構造変更アナログ脂質の合成と二重膜物性, 日本膜学会第43年会, Jun. 2021.
44.	Toshiki Nakao, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : Organic Synthesis and Bilayer Properties of a Sphingolipid Analog, an Amide-Linked Phosphatidylcholine, 12th Annual Meeting of Chugoku/Shikoku Branch in the Biophysical Society of Japan, May 2021.
45.	荒井祐徹, 今村比呂志, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and 加藤稔 : ジミリストイルホスファチジルコリン二重膜の温度・圧力相転移に関するFTIR研究, 第61回高圧討論会, Dec. 2020.
46.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 飽和ジアルキルホスファチジルコリン二重膜の熱力学的相図, 第61回高圧討論会, Dec. 2020.
47.	長安真舜, 川原宏介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧力が誘起する不飽和リン脂質混合二重膜の膜融合とその分子メカニズム, 2020年度日本化学会中国四国支部大会, Nov. 2020.
48.	神谷芽生, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 非対称型カチオン-アニオン界面活性剤の水中での会合体形成, 2020年度日本化学会中国四国支部大会, Nov. 2020.
49.	尾形茉実, 本橋牧子, 青木雄椰, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ハロゲン化ジドデシルジメチルアンモニウム二重膜相挙動におよぼす対イオン効果, 2020年度日本化学会中国四国支部大会, Nov. 2020.
50.	Nobutake Tamai, 清水建翔, 横矢祐香, Masaki GOTO and Hitoshi Matsuki : 対称型カチオン–アニオン界面活性剤塩の水中での会合体形成に関する熱力学的研究, 膜シンポジウム2020, Nov. 2020.
51.	中尾俊樹, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : スフィンゴリン脂質類似アミド型リン脂質二重膜の熱的相転移:鎖結合様式の対照的効果, 第56回熱測定討論会, Oct. 2020.
52.	中尾俊樹, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : スフィンゴ脂質類似アミド結合型ホスファチジルコリンの有機合成と二重膜物性, 日本膜学会第42年会, Jun. 2020.
53.	川原宏介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧力下における不飽和リン脂質混合二重膜ベシクルの融合条件, 2019年度日本化学会中国四国支部大会, Nov. 2019.
54.	中尾俊樹, Masashi Kurashina, Masaki GOTO, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki : アミド結合型ホスファチジルコリンの有機合成および二分子膜相転移評価, 2019年度日本化学会中国四国支部大会, Nov. 2019.
55.	野村拓弘, 成瀬由希, 岡本裕嗣, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧蛍光法によるジパルミトイルホスファチジルグリセロール二重膜内充填構造の評価, 2019年度日本化学会中国四国支部大会, Nov. 2019.
56.	田中杏奈, 本橋牧子, 青木雄椰, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 塩化ジアルキルジメチルアンモニウムの合成と二重膜相挙動の解明, 2019年度日本化学会中国四国支部大会, Nov. 2019.
57.	吉田俊太郎, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧力下におけるDPPC二重膜のエタノール誘起指組み構造形成, 2019年度日本化学会中国四国支部大会, Nov. 2019.
58.	原山侑也, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 荷電状態の異なる極性頭部転置型リン脂質二分子膜の合成と膜物性, 2019年度日本化学会中国四国支部大会, Nov. 2019.
59.	木口碧, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 中鎖ホスファチジルコリン二重膜のゲル-液晶中間相状態, 2019年度日本化学会中国四国支部大会, Nov. 2019.
60.	平井悠貴, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : ハロゲン化ジアルキルジメチルアンモニウム二分子膜の体積挙動, 2019年度日本化学会中国四国支部大会, Nov. 2019.
61.	清水建翔, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 水中における対称型カチオン-アニオン界面活性剤の会合体形成, 2019年度日本化学会中国四国支部大会, Nov. 2019.
62.	Nobutake Tamai, 稲澤早苗, Masaki GOTO and Hitoshi Matsuki : 植物および菌類由来のステロールが誘起するリン脂質二分子膜側方相分離挙動, 膜シンポジウム2019, Nov. 2019.
63.	Hitoshi Matsuki, 木口碧, 中尾俊樹, 本橋牧子, Masaki GOTO and Nobutake Tamai : 中鎖ホスファチジルコリン二重膜のゲル-液晶中間状態:出現様式と形成機構, 膜シンポジウム2019, Nov. 2019.
64.	Masaki GOTO, 岡本裕嗣, 成瀬由希, 野村拓弘, Nobutake Tamai and Hitoshi Matsuki : ジステアロイルホスファチジルコリン二重膜の高圧相挙動, 第60回高圧討論会, Oct. 2019.
65.	成瀬由希, 中江ひかる, 山口舞夏, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質ジパルミトイルホスファチジン酸二重膜の熱的相転移, 第55回熱測定討論会, Oct. 2019.
66.	横矢祐香, 桶谷嘉一, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 対称型カチオン-アニオン界面活性剤塩の水中における二分子膜状会合体形成, 第55回熱測定討論会, Oct. 2019.
67.	本橋牧子, 青木雄椰, 田中杏奈, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 塩化ジアルキルジメチルアンモニウム二重膜の相転移熱力学量, 第55回熱測定討論会, Oct. 2019.
68.	Nobutake Tamai, 稲澤早苗, Masaki GOTO and Hitoshi Matsuki : リン脂質二分子膜の相挙動におよぼす種々のステロールの効果, 第55回熱測定討論会, Oct. 2019.
69.	Nobutake Tamai, 信岡健, 武知嶺, Masaki GOTO and Hitoshi Matsuki : 動的および静的光散乱法によるリン脂質ベシクルの構造評価2, 第57回生物物理学会年会, Sep. 2019.
70.	Nobutake Tamai, 信岡健, 武知嶺, Masaki GOTO and Hitoshi Matsuki : 動的・静的光散乱法によるリン脂質会合体の構造特性評価, 日本膜学会第41年会, May 2019.
71.	Hitoshi Matsuki, 田中佐江子, 岡本裕嗣, Masaki GOTO and Nobutake Tamai : 酸性リン脂質二分子膜の会合形態依存相転移, 日本膜学会第41年会, May 2019.
72.	Masaki GOTO, 岡本裕嗣, 吉本早希, 吉田俊太郎, Nobutake Tamai and Hitoshi Matsuki : リン脂質二分子膜の圧力誘起相転移 -アシル鎖長の異なる飽和ジアシルホスファチジルグリセロール-, 第59回高圧討論会, Nov. 2018.
73.	吉田俊太郎, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ホスファチジルコリン二重膜の圧力誘起指組み構造形成:高圧蛍光法による遷移状態の実証, 第59回高圧討論会, Nov. 2018.
74.	青木雄椰, 村上尚, 村上祐介, 田中杏奈, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 陽イオン性二本鎖界面活性剤二重膜の相転移に関する熱力学的研究, 第59回高圧討論会, Nov. 2018.
75.	平井悠貴, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 核磁気共鳴法によるホスファチジルコリン二分子膜中の脂質分子運動性評価, 2018年度日本化学会中国四国支部大会, Nov. 2018.
76.	清水建翔, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : カチオン-アニオン界面活性剤の水中における会合挙動, 2018年度日本化学会中国四国支部大会, Nov. 2018.
77.	田中杏奈, 青木雄椰, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 塩化テトラデシルジメチルアンモニウムの合成と二分子膜物性, 2018年度日本化学会中国四国支部大会, Nov. 2018.
78.	Hitoshi Matsuki, 風間暁, Masaki GOTO and Nobutake Tamai : 脂質二分子膜の圧力誘起膜融合2:融合の分子メカニズム, 膜シンポジウム2018, Nov. 2018.
79.	Hitoshi Matsuki, 木口碧, 中尾俊樹, 本橋牧子, Masaki GOTO and Nobutake Tamai : リン脂質二分子膜のゲル-液晶中間相形成:充填状態依存相転移と形成機構, 第54回熱測定討論会, Oct. 2018.
80.	吉田俊太郎, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ホスファチジルコリン二重膜の圧力誘起指組み構造形成:蛍光プローブ法による遷移状態の検出, 第35回九州コロイドコロキウム, Sep. 2018.
81.	木口碧, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 中鎖ホスファチジルコリン二分子膜の特異的な相転移:充填状態に依存したゲル-液晶転移, 第35回九州コロイドコロキウム, Sep. 2018.
82.	Masaki GOTO, 村上祐介, 村上尚, Masaki GOTO and Hitoshi Matsuki : 塩化ジアルキルジメチルアンモニウム二重膜の高圧相挙動:アニーリング処理に依存した膜状態, 第69回コロイドおよび界面化学討論会, Sep. 2018.
83.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : リン脂質-コレステロール混合二分子膜に対するコレステロール依存性細胞溶解毒素の結合活性, 第69回コロイドおよび界面化学討論会, Sep. 2018.
84.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : モデル膜を用いたコレステロール依存性細胞溶解毒素の膜結合活性評価, 第56回生物物理学会年会, Sep. 2018.
85.	木口碧, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 中鎖ホスファチジルコリン二分子膜のゲル-液晶中間相形成, 第10回日本生物物理学会中国四国支部大会, May 2018.
86.	吉田俊太郎, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ホスファチジルコリン二分子膜における圧力誘起指組み構造形成の分子機構, 第10回日本生物物理学会中国四国支部大会, May 2018.
87.	平井悠貴, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : エステルおよびエーテル結合型ホスファチジルコリン二分子膜中の脂質分子運動性評価, 第10回日本生物物理学会中国四国支部大会, May 2018.
88.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 脂質二分子膜の圧力誘起膜融合, 日本膜学会第40年会, May 2018.
89.	Nobutake Tamai, 森光達, Masaki GOTO, Hideaki Nagamune and Hitoshi Matsuki : コレステロール依存性細胞溶解毒素の脂質膜に対する結合活性, 日本膜学会第40年会, May 2018.
90.	Hitoshi Matsuki, 吉田俊太郎, Masaki GOTO and Nobutake Tamai : 脂質二分子膜へのリガンド分配2:温度・圧力誘起相の安定性変化, 膜シンポジウム2017, Nov. 2017.
91.	竹下真広, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : リン脂質–コレステロール二成分混合二分子膜における各成分の部分モル体積, 膜シンポジウム2017, Nov. 2017.
92.	村上祐介, 村上尚, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 塩化ジアルキルジメチルアンモニウム二重膜の高圧相挙動, 第58回高圧討論会, Nov. 2017.
93.	Masaki GOTO, 吉田俊太郎, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホスファチジルグリセロール二重膜の蛍光イメージング, 第58回高圧討論会, Nov. 2017.
94.	Hitoshi Matsuki, 岡本裕嗣, 吉田俊太郎, Masaki GOTO and Nobutake Tamai : リガンド存在下におけるリン脂質二分子膜の高圧相挙動:圧力誘起指組み構造ゲル相の安定性変化, 第58回高圧討論会, Nov. 2017.
95.	中尾俊樹, 木口碧, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジトリデカノイルホスファチジルコリン二分子膜における中間相の熱的相転移, 第53回熱測定討論会, Nov. 2017.
96.	Masaki GOTO, 岡本裕嗣, 中尾俊樹, Nobutake Tamai and Hitoshi Matsuki : リガンド添加による脂質二分子膜相転移温度変化の熱力学的解釈, 第55回生物物理学会年会, Sep. 2017.
97.	Nobutake Tamai, 泉川拓也, 植村麻衣子, Masaki GOTO and Hitoshi Matsuki : コレステロールによるジアルキルホスファチジルコリンの指組構造化の抑制, 第55回生物物理学会年会, Sep. 2017.
98.	村上尚, 村上祐介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 二本鎖陽イオン性界面活性剤二重膜の高圧相挙動, 第68回コロイドおよび界面化学討論会, Sep. 2017.
99.	坪井俊祐, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : コレステロールを含む二成分リン脂質二分子膜の圧力摂動熱量測定を用いた体積挙動の定量的評価, 第68回コロイドおよび界面化学討論会, Sep. 2017.
100.	岡本裕嗣, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and 深田和宏 : 添加塩により誘起されるジステアロイルホスファチジルグリセロール二分子膜の特異的相転移, 第68回コロイドおよび界面化学討論会, Sep. 2017.
101.	Masaki GOTO, 村上尚, 村上祐介, Masaki GOTO and Hitoshi Matsuki : 塩化ジアルキルジメチルアンモニウム二重膜の圧力誘起相転移, 第68回コロイドおよび界面化学討論会, Sep. 2017.
102.	Nobutake Tamai, 泉川拓也, 植村麻衣子, Masaki GOTO and Hitoshi Matsuki : ジアルキルリン脂質の指組構造形成におよぼすコレステロール効果, 第68回コロイドおよび界面化学討論会, Sep. 2017.
103.	Hitoshi Matsuki, 岡本裕嗣, Masaki GOTO and Nobutake Tamai : 脂質二分子膜へのリガンド分配:相転移温度変化の熱力学的考察, 日本膜学会第39年会, May 2017.
104.	Nobutake Tamai, 泉川拓也, 植村麻衣子, Masaki GOTO and Hitoshi Matsuki : エーテル結合型リン脂質-コレステロール二成分混合膜の相挙動, 日本膜学会第39年会, May 2017.
105.	青木雄椰, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : N-メチル化ジヘキサデシルホスファチジルエタノールアミン二分子膜の高圧相挙動, 第9回日本生物物理学会中国四国支部大会, May 2017.
106.	Masaki GOTO, 岡本裕嗣, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホスファチジルグリセロール二重膜の圧力誘起相転移, 第9回日本生物物理学会中国四国支部大会, May 2017.
107.	岡本裕嗣, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジアシルホスファチジルグリセロール二分子膜の添加塩誘起相転移, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
108.	竹下真広, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : コレステロール含有二成分リン脂質二分子膜中における構成脂質の部分モル体積の組成依存的変化, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
109.	Masaki GOTO, 遠藤茂, Nobutake Tamai and Hitoshi Matsuki : アシル鎖長の異なるホスファチジルエタノールアミン二重膜の熱および圧力相転移, 第54回生物物理学会年会, Nov. 2016.
110.	Nobutake Tamai, 竹下真広, Masaki GOTO and Hitoshi Matsuki : ジミリストイルホスファチジルコリン-コレステロール二成分二分子膜中における構成脂質の部分モル体積, 第54回生物物理学会年会, Nov. 2016.
111.	竹下真広, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : リン脂質-コレステロール混合二分子膜の体積挙動:部分モル体積の組成依存性, 2016年度日本化学会中国四国支部大会, Nov. 2016.
112.	坪井俊祐, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : リン脂質-コレステロール混合二分子膜の体積挙動:転移体積の組成依存性, 2016年度日本化学会中国四国支部大会, Nov. 2016.
113.	村上祐介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 二本鎖陽イオン性界面活性剤二重膜の相挙動-アルキル鎖長の影響-, 2016年度日本化学会中国四国支部大会, Nov. 2016.
114.	村上尚, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 二本鎖陽イオン性界面活性剤二重膜の相挙動-対イオンの影響-, 2016年度日本化学会中国四国支部大会, Nov. 2016.
115.	Masaki GOTO, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : 陽イオン性二本鎖界面活性剤二重膜の構造解析, 2016年度日本化学会中国四国支部大会, Nov. 2016.
116.	Masaki GOTO, 岡本裕嗣, Nobutake Tamai and Hitoshi Matsuki : 高圧力下におけるジパルミトイルホスファチジルグリセロール二重膜の相挙動, 第57回高圧討論会, Oct. 2016.
117.	Hitoshi Matsuki, 山下翔子, Masaki GOTO and Nobutake Tamai : 飽和ジアシルホスファチジルエタノールアミン二重膜の相転移:疎水鎖長依存性と相転移熱力学量, 第57回高圧討論会, Oct. 2016.
118.	Masaki GOTO, 岡本裕嗣, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホスファチジルグリセロール二重膜の相転移熱力学量, 第52回熱測定討論会, Sep. 2016.
119.	西本真琴, 高垣美由紀, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and 山中美智男 : 血清アルブミンと麻酔薬の本質的な結合様式:麻酔薬疎水性と特異的結合部位, 第52回熱測定討論会, Sep. 2016.
120.	村上祐介, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 二本鎖陽イオン性界面活性剤二重膜の相挙動におよぼすアルキル鎖長の影響, 第52回熱測定討論会, Sep. 2016.
121.	坪井俊祐, Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 圧力摂動熱量測定を用いたジミリストイルホスファチジルコリン-コレステロール混合二分子膜の体積挙動の定量的評価, 第52回熱測定討論会, Sep. 2016.
122.	村上尚, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 二本鎖陽イオン性界面活性剤二重膜の相挙動におよぼす対イオンの影響, 第52回熱測定討論会, Sep. 2016.
123.	岡本裕嗣, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジアシルホスファチジルグリセロール二分子膜が形成する棒状会合体のゲル-液晶転移, 第52回熱測定討論会, Sep. 2016.
124.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 圧力摂動熱量法を利用したリン脂質二分子膜の体積特性の評価, 第52回熱測定討論会, Sep. 2016.
125.	Nobutake Tamai, 竹下真広, 坪井俊祐, Masaki GOTO and Hitoshi Matsuki : ジミリストイルホスファチジルコリン二分子膜の体積特性におよぼすコレステロール効果, 第67回コロイドおよび界面化学討論会, Sep. 2016.
126.	Hitoshi Matsuki, 山下翔子, Masaki GOTO and Nobutake Tamai : 飽和ジアシルホスファチジルエタノールアミン二重膜の温度および圧力誘起相転移, 第67回コロイドおよび界面化学討論会, Sep. 2016.
127.	Nobutake Tamai, 坪井俊祐, 竹下真広, Masaki GOTO and Hitoshi Matsuki : ジミリストイルホスファチジルコリン-コレステロール混合二分子膜の体積挙動, 第8回日本生物物理学会中国四国支部大会, May 2016.
128.	岡本裕嗣, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジステアロイルホスファチジルグリセロール二分子膜の添加塩濃度に依存した特異的な相転移, 第8回日本生物物理学会中国四国支部大会, May 2016.
129.	Hitoshi Matsuki, 加藤謙太郎, Masaki GOTO and Nobutake Tamai : 高圧力下におけるリン脂質二重膜のリガンド分配様式, 第56回高圧討論会, Nov. 2015.
130.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜の圧力誘起相転移–酸性リン脂質ジパルミトイルホスファチジルグリセロール–, 第56回高圧討論会, Nov. 2015.
131.	Masaki GOTO : Evaluation of Phospholipid Bilayer Packing by High-pressure Fluorometry, 第38回溶液化学シンポジウムプレシンポジウム, Oct. 2015.
132.	井上嶺之, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質二重膜の温度・圧力誘起相転移:ジミリストイルホスファチジルグリセロール, 第51回熱測定討論会, Oct. 2015.
133.	黒葛和信, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 血清アルブミン中における麻酔薬の結合部位:熱的および構造データの相関性, 第51回熱測定討論会, Oct. 2015.
134.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : Temperature- and pressure-induced bilayer phase transitions of phosphatidylcholines with asymmetric saturated acyl chains, 第53回日本生物物理学会年会, Sep. 2015.
135.	Masaki GOTO, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : 高圧小角中性子散乱法による二本鎖界面活性剤会合体の構造解析, 第66回コロイドおよび界面化学討論会, Sep. 2015.
136.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 生体膜脂質の非対称性:非対称飽和ホスファチジルコリン位置異性体, 特殊環境微生物セミナー2015, Sep. 2015.
137.	Masaki GOTO : 支部発海外だより∼スイス・ポールシェラー研究所滞在記∼, 日本生物物理学会第7回中国四国支部大会, May 2015.
138.	黒葛和信, Masaki GOTO, Nobutake Tamai, 西本真琴 and Hitoshi Matsuki : 血清アルブミン中における麻酔薬の結合部位:脂肪酸の結合部位との同一性, 第7回日本生物物理学会中国四国支部大会, May 2015.
139.	井上嶺之, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 酸性リン脂質二重幕の熱的相転移:ジミリストイルホスファチジルグリセロール, 第7回日本生物物理学会中国四国支部大会, May 2015.
140.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 圧力摂動熱量法を用いたリン脂質二分子膜の体積挙動の定量的評価, 日本膜学会第37年会, May 2015.
141.	Hitoshi Matsuki, 矢野貴大, Masaki GOTO and Nobutake Tamai : 脂質膜の圧力誘起指組み構造形成(その2):アシル鎖非対称性, 日本膜学会第37年会, May 2015.
142.	八木信久, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧下におけるリン脂質二分子膜の体積挙動 ―高圧精密密度測定システムの構築―, 日本膜学会第37年会, May 2015.
143.	井上嶺之, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ホスファチジルグリセロール二分子膜の熱的相挙動:相転移におよぼす電荷の影響, 日本膜学会第37年会, May 2015.
144.	茨木孝司, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜の圧力誘起膜融合 -融合率と封入率の相関性-, 第55回高圧討論会, Nov. 2014.
145.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : リン脂質二分子膜の体積挙動, 第55回高圧討論会, Nov. 2014.
146.	矢野貴大, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質二分子膜の圧力誘起相転移 -非対称飽和アシル鎖をもつホスファチジルコリン-, 第55回高圧討論会, Nov. 2014.
147.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : リン脂質二重膜の圧力および化学誘起指組み構造化:形成機構の相違, 第52回生物物理学会年会, Sep. 2014.
148.	Nobutake Tamai, 信岡健, Masaki GOTO and Hitoshi Matsuki : 動的および静的光散乱法によるリン脂質ベシクルの構造評価, 第52回生物物理学会年会, Sep. 2014.
149.	Nobutake Tamai, 森絵里香, Masaki GOTO and Hitoshi Matsuki : リン脂質ベシクルの体積緩和挙動におよぼすコレステロール効果, 第65回コロイドおよび界面化学討論会, Sep. 2014.
150.	Hitoshi Matsuki, 金澤雅俊, Masaki GOTO and Nobutake Tamai : 高圧力が誘起するリン脂質二重膜の膜融合:膜曲率の効果と融合機構, 第65回コロイドおよび界面化学討論会, Sep. 2014.
151.	矢野貴大, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 脂質二分子膜相安定性におよぼすアシル鎖非対称性の影響, 第6回日本生物物理学会中国四国支部大会, May 2014.
152.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 脂質膜の圧力誘起指組み構造形成:短鎖限界と長鎖限界, 日本膜学会第36年会, May 2014.
153.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : コレステロールが誘起するジパルミトイルホスファチジルコリン二重膜の側方相分離, 日本膜学会第36年会, May 2014.
154.	Hitoshi Matsuki, 多田佳織, Masaki GOTO and Nobutake Tamai : リン脂質二分子膜の圧力誘起相転移 -ジラウロイル基を有するリン脂質-, 第54回高圧討論会, Nov. 2013.
155.	Masaki GOTO, 佐藤明弘, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜のサブゲル相形成 -エーテル結合およびエステル結合型リン脂質の相違-, 第54回高圧討論会, Nov. 2013.
156.	西本真琴, 矢野華奈子, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ホタル発光酵素ルシフェラーゼへの麻酔薬効果:DSC測定による構造安定性の評価, 第49回熱測定討論会, Nov. 2013.
157.	Hitoshi Matsuki, 黒葛和信, Masaki GOTO, Nobutake Tamai and 西本真琴 : 牛血清アルブミンへの麻酔薬効果:DSC，ITCおよびPPC測定による比較研究, 第49回熱測定討論会, Nov. 2013.
158.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧蛍光法により明らかにされるサブゲル相中のホスファチジルコリン分子のスタッガード構造, 第51回生物物理学会年会, Oct. 2013.
159.	Nobutake Tamai, 稲澤早苗, 藤原大樹, Masaki GOTO and Hitoshi Matsuki : ジパルミトイルホスファチジルコリン二分子膜の熱的相挙動に及ぼすステロール効果, 第51回生物物理学会年会, Oct. 2013.
160.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 疎水性相互作用に支配される脂質膜の圧力誘起指組み構造形成, 特殊環境微生物セミナー2013, Oct. 2013.
161.	茨木孝司, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 圧力誘起膜融合リポソームの安定性評価, 第64回コロイドおよび界面化学討論会, Sep. 2013.
162.	矢野貴大, 森神佳彦, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 非対称型ホスファチジルコリン二分子膜の高圧誘起相転移, 第64回コロイドおよび界面化学討論会, Sep. 2013.
163.	大坪洋平, 岩佐悠太, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ハロゲン化ジオクタデシルジメチルアンモニウム二重膜の高圧相挙動, 第64回コロイドおよび界面化学討論会, Sep. 2013.
164.	Masaki GOTO, Agnieszka Wilk, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : ホスファチジルコリン二分子膜における圧力誘起指組み構造の分子メカニズム, 第64回コロイドおよび界面化学討論会, Sep. 2013.
165.	Nobutake Tamai, 柿部小百合, 田中佐江子, Masaki GOTO and Hitoshi Matsuki : 圧力摂動熱量法によるリン脂質二分子膜の緩和挙動の観測, 第64回コロイドおよび界面化学討論会, Sep. 2013.
166.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 高圧力が誘起するリン脂質二重膜の膜融合, 第64回コロイドおよび界面化学討論会, Sep. 2013.
167.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 高圧力により誘起されるリン脂質二重膜の膜融合(第2報), 第18回生物関連高圧研究会シンポジウム, Sep. 2013.
168.	Hitoshi Matsuki, 西本真琴, Masaki GOTO and Nobutake Tamai : 麻酔作用のタンパク質受容体仮説の源泉:ホタル発光酵素ルシフェラーゼ, 第5回日本生物物理学会中国四国支部大会, May 2013.
169.	Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki : 脂質ラフト形成は熱力学的に説明できるのか? -側方相分離とクラスター形成-, 第5回日本生物物理学会中国四国支部大会, May 2013.
170.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質二重膜のイメージング:蛍光分子の膜内配向で見る膜状態, 第5回日本生物物理学会中国四国支部大会, May 2013.
171.	Masaki GOTO, Agnieszka Wilk, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : リン脂質二重膜のサブゲル相形成 -非対称型リン脂質膜への高圧蛍光法の適用-, 第53回高圧討論会, Nov. 2012.
172.	Hitoshi Matsuki, 金澤雅俊, Masaki GOTO and Nobutake Tamai : リン脂質二重膜の圧力誘起膜融合 -巨大単層ベシクルの不可逆的球形成長-, 第53回高圧討論会, Nov. 2012.
173.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : リン脂質二重膜の圧力誘起指組み構造形成:疎水鎖長依存性と形成限界, 第50回生物物理学会年会, Sep. 2012.
174.	Nobutake Tamai, 柿部小百合, 田中佐江子, Masaki GOTO and Hitoshi Matsuki : 圧力摂動熱量法によるジミリストイルホスファチジルコリン二分子膜の緩和挙動の解明への試み, 第50回生物物理学会年会, Sep. 2012.
175.	Nobutake Tamai, 稲澤早苗, 藤原大樹, Masaki GOTO and Hitoshi Matsuki : エルゴステロール–ジパルミトイルホスファチジルコリン二成分混合二分子膜の相挙動, 第4回日本生物物理学会中国四国支部大会, Jun. 2012.
176.	Masaki GOTO, Agnieszka Wilk, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : ジアシルホスファチジルコリン二重膜の圧力誘起指組み構造化の形成限界, 第4回日本生物物理学会中国四国支部大会, Jun. 2012.
177.	Makoto Fukui, SA Mulyatno, Masaki GOTO, Kaname Miki, RC Orihuela-Campos, Kosuke Kataoka, 林田秀明, 北村雅保, 川崎浩二, 関田孝晴, 中里未央, 前田隆浩, 齋藤俊行 and Hiro-O Ito : ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性-第二報-, 第61回日本口腔衛生学会・総会, May 2012.
178.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 脂質膜の圧力誘起指組み構造形成:疎水鎖長依存性と形成限界, 材料学会第61期通常総会講演会極限環境フォーラム, May 2012.
179.	森神佳彦, 田中佐江子, 澤口裕史, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジアシルホスファチジルコリン二分子膜のラメラ結晶相形成におよぼすアシル鎖長の効果, 2011年日本化学会西日本大会, Nov. 2011.
180.	田岡昌朗, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジアシルホスファチジルコリン二分子膜の指組み構造化のメカニズム, 2011年日本化学会西日本大会, Nov. 2011.
181.	田中佐江子, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホスファチジルグリセロール二分子膜の相挙動に及ぼすNaClの効果, 2011年日本化学会西日本大会, Nov. 2011.
182.	金澤雅俊, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 非対称型リン脂質二分子膜相挙動におよぼすベシクルサイズの効果, 2011年日本化学会西日本大会, Nov. 2011.
183.	稲澤早苗, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホスファチジルコリン二分子膜相挙動に及ぼすスティグマステロールの効果, 2011年日本化学会西日本大会, Nov. 2011.
184.	遠藤卓也, 田中佐江子, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 長鎖飽和アシル鎖を有するホスファチジルコリン二分子膜の高圧相挙動, 2011年日本化学会西日本大会, Nov. 2011.
185.	岩佐悠太, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 臭化および塩化ジオクタデシルジメチルアンモニウム二分子膜相挙動におよぼす1-ブタノールの影響, 2011年日本化学会西日本大会, Nov. 2011.
186.	Nobutake Tamai, 南部優子, Masaki GOTO and Hitoshi Matsuki : 圧力摂動法による脂質二分子膜体積挙動の定量的解析, 第52回高圧討論会, Nov. 2011.
187.	Masaki GOTO, Agnieszka Wilk, 遠藤卓也, 田中佐江子, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki : 長鎖アシル鎖を有するホスファチジルコリン二分子膜の高圧相挙動, 第52回高圧討論会, Nov. 2011.
188.	Makoto Fukui, Sapta Adisuka Mulyatno, バータルジャフツェルメグ, Masaki GOTO, Kosuke Kataoka, 林田秀明, 川崎浩二, 前田隆浩, 中里未央, 齋藤俊行 and Hiro-O Ito : ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性:五島研究, Oct. 2011.
189.	Sapta Adisuka Mulyatno, Kosuke Kataoka, 関根伸一, バータルジャブツェルメグ, Makoto Fukui, 金川裕子, Masaki GOTO and Hiro-O Ito : 経鼻粘膜アジュバントCpG ODNはマクロファージのTLR9発現と歯周病細菌抗原特異的Th1免疫応答を誘導する, Oct. 2011.
190.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 高圧力により誘起されるリン脂質二重膜の膜融合, 第17回生物関連高圧研究会シンポジウム, Sep. 2011.
191.	Nobutake Tamai, 南部優子, Masaki GOTO and Hitoshi Matsuki : 圧力摂動法によるエステルおよびエーテル結合型リン脂質膜の体積挙動の解明, 第3回日本生物物理学会中国四国支部大会, May 2011.
192.	Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai : 脂質二重膜内パッキング状態のイメージング解析, 第3回日本生物物理学会中国四国支部大会, May 2011.
193.	梨野翔, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : リン脂質-短鎖脂肪酸混合二分子膜の相挙動, 2010年日本化学会西日本大会, Nov. 2010.
194.	衣川進, 多田佳織, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : カチオン-アニオン界面活性剤混合系の会合体転移に関する熱的研究, 2010年日本化学会西日本大会, Nov. 2010.
195.	Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina : ジアシルホスファチジルコリン二分子膜相挙動の蛍光イメージング解析, 第51回高圧討論会, Oct. 2010.
196.	風間暁, Agnieszka Wilk, Masaki GOTO, Nobutake Tamai, Shoji Kaneshina, Joachim Kohlbrecher and Hitoshi Matsuki : ホスファチジルコリン二分子膜の圧力誘起指組み構造化のメカニズム, 第51回高圧討論会, Oct. 2010.
197.	Nobutake Tamai, 泉川拓也, Masaki GOTO and Hitoshi Matsuki : ジヘキサデシルホスファチジルコリン二分子膜の相挙動に及ぼすコレステロールの影響, 第2回日本生物物理学会中国四国支部大会, May 2010.
198.	筒井舞子, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 飽和・不飽和リン脂質二分子膜の体積挙動観測, 第2回日本生物物理学会中国四国支部大会, May 2010.
199.	田中佐江子, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : ジパルミトイルホファチジルグリセロール二分子膜の高圧相挙動, 第2回日本生物物理学会中国四国支部大会, May 2010.

Et cetera, Workshop:

1.	Kazuya Kusaka, Yohei Yamada, 大西舞, Masayuki Tsukagoshi, Mikito Yasuzawa, Masaki GOTO, Emi Morimoto, Masatake Akutagawa, Kenji Terada and Shoichiro Fujisawa : Enfocement and evaluation of the introductory subject "project menagement fundamentals", 電気学会研究会資料制御研究会, Vol.CT-14, No.15, 11-16, Apr. 2014. (Summary) We report about the introductory subject "Project Management Fundamentals". The student will get a basic ability to work with diverse peoples in the community or workplace, through the planning and practice of a project to accomplish the task. In addition, the student will obtain project management skills required to design and implement the project. This year's theme was "the development of new products to eliminate the inconvenience for you". The students worked on the development of new products in a team as a project. We evaluated the individual and team by using the project notes. (Keyword) Introductory subject / Creation subject / Project / project management / Group activity / evaluation

Patent:

1.	Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki : 高圧力を利用したリポソームの粒子径制御方法, 2011-021310 (Feb. 2011), .

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Synthesis of non-native phospholipids with an inverted polar headgroup by lipidmutation and their aggregation behavior (Project/Area Number: 21K05006 )
Bilayer characterization of analog lipids having modified module structure (Project/Area Number: 19K05404 )
Mechano-asymmetry of lipid membranes: lipid molecular asymmetry revealed from high-pressure study (Project/Area Number: 26410016 )
Are inhalation anesthetics really receptor-binding drugs? : calorimetry of firefly luciferase (Project/Area Number: 24659698 )
Volume relaxation behavior of sterol-containing lipid bilayers and dynamic mechanism of the formation of microdomains. (Project/Area Number: 24550157 )
Relationship between Mucosal Immune Response to Phosphorylcholineand atherosclerosis (Project/Area Number: 23792515 )
Search by Researcher Number (30507455)

Associate Professor : GOTO, Masaki

Research

Personal Web Page

Field of Study

Subject of Study

Book / Paper

Book:

Academic Paper (Judged Full Paper):

Academic Paper (Unrefereed Paper):

Review, Commentary:

Proceeding of International Conference:

Proceeding of Domestic Conference:

Et cetera, Workshop:

Patent:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)