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徳島大学バイオイノベーション研究所
徳島大学研究クラスター群研究クラスター群 (指定)2001001 ゲノム編集技術を活用した異種キメラブタの開発
徳島大学先端技術科学教育部物質生命システム工学専攻生命テクノサイエンスコース生物反応工学講座
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研究活動

個人のホームページ

専門分野

家畜繁殖学

研究テーマ

生殖工学を活用した野生動物の保護と形質転換動物の作出

著書・論文

学術論文(審査論文):

1. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.,
International Journal of Molecular Sciences, Vol.22, No.5, 2249, 2021.
(DOI: 10.3390/ijms22052249,   PubMed: 33668187,   Elsevier: Scopus)
2. Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Le Anh, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara and Takeshige Otoi :
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.,
Animals : An Open Access Journal from MDPI, Vol.11, No.2, 2021.
(DOI: 10.3390/ani11020578,   PubMed: 33672168,   Elsevier: Scopus)
3. Anh Quynh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata and Takeshige Otoi :
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.,
BMC research notes, Vol.14, No.1, 7, 2021.
(DOI: 10.1186/s13104-020-05412-8,   PubMed: 33407863)
4. Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, T Nhien Nguyen, A Quynh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.,
Animal Science Journal, Vol.92, No.1, e13534, 2021.
(DOI: 10.1111/asj.13534,   PubMed: 33638256)
5. Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi and Takeshige Otoi :
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.8, 614-621, 2020.
(DOI: 10.1007/s11626-020-00507-9,   PubMed: 32978715)
6. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi :
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes.,
BMC Biotechnology, Vol.20, No.1, 40, 2020.
(DOI: 10.1186/s12896-020-00638-7,   PubMed: 32811500)
7. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Thi Nhien Nguyen, Koki Takebayashi, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation.,
Molecular Biology Reports, Vol.47, No.7, 5073-5079, 2020.
(DOI: 10.1007/s11033-020-05576-3,   PubMed: 32519310)
8. Takayuki Hirano, Maki Hirata, Shigeyuki Fujimoto, Thi Nhien Nguyen, Le Anh Quynh, Fuminori Tanihara and Takeshige Otoi :
Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.4, 277-280, 2020.
(DOI: 10.1007/s11626-020-00451-8,   PubMed: 32394241)
9. Fuminori Tanihara, Maki Hirata, Nhien Nguyen Thi, Le Anh Quynh, Takayuki Hirano and Takeshige Otoi :
Generation of viable PDX1 gene-edited founder pigs as providers of nonmosaics.,
Molecular Reproduction and Development, Vol.87, No.4, 471-481, 2020.
(DOI: 10.1002/mrd.23335,   PubMed: 32166879)
10. Yoko Sato, Kiyoshi Asahina, Miki Yoshiike, Shiari Nozawa, Takeshige Otoi and Teruaki Iwamoto :
A change in the steroid metabolic pathway in human testes showing deteriorated spermatogenesis.,
Reproductive Biology, Vol.20, No.2, 210-219, 2020.
(DOI: 10.1016/j.repbio.2020.02.008,   PubMed: 32151535)
11. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi :
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020.
(DOI: 10.1111/rda.13609,   PubMed: 31858644)
12. A Quynh Le, Maki Hirata, T Nhien Nguyen, Koki Takebayashi, Manita Wittayarat, Yoko Sato, Zhao Namula, Masahiro Nii, Fuminori Tanihara and Takeshige Otoi :
Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes.,
Animal Science Journal, Vol.91, No.1, e13386, 2020.
(DOI: 10.1111/asj.13386,   PubMed: 32512638)
13. Shinya Ishihara, Q Thanh Dang-Nguyen, Kazuhiro Kikuchi, Aisaku Arakawa, Satoshi Mikawa, Makoto Osaki, Takeshige Otoi, Minh Quang Luu, Son Thanh Nguyen and Masaaki Taniguchi :
Characteristic features of porcine endogenous retroviruses in Vietnamese native pigs.,
Animal Science Journal, Vol.91, No.1, e13336, 2020.
(DOI: 10.1111/asj.13336,   PubMed: 32219916)
14. Ha Minh Ngo, MaríaCruz Arnal, Ryosuke Sumi, Junna Kawasaki, Ariko Miyake, K Chris Grant, Takeshige Otoi, Daniel de Luco Fernández and Kazuo Nishigaki :
Tracking the Fate of Endogenous Retrovirus Segregation in Wild and Domestic Cats.,
Journal of Virology, Vol.93, No.24, 2019.
(DOI: 10.1128/JVI.01324-19,   PubMed: 31534037)
15. Thanh-Van Nguyen, Kim Lanh Thi Do, Tamas Somfai, Takeshige Otoi, Masayasu Taniguchi and Kazuhiro Kikuchi :
Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress.,
Animal Science Journal, Vol.90, No.12, 1530-1536, 2019.
(DOI: 10.1111/asj.13302,   PubMed: 31663235)
16. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Le Anh Quynh, Manita Wittayarat, Mokhamad Fahrudin, Takayuki Hirano and Takeshige Otoi :
Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes.,
Animal Biotechnology, 2019.
(DOI: 10.1080/10495398.2019.1668801,   PubMed: 31558095)
17. Maki Hirata, Manita Wittayarat, Takayuki Hirano, Thi Nhien Nguyen, Le Anh Quynh, Zhao Namula, Mokhamad Fahrudin, Fuminori Tanihara and Takeshige Otoi :
The relationship between embryonic development and the efficiency of target mutations in porcine endogenous retroviruses (PERVs) pol genes in porcine embryos.,
Animals : An Open Access Journal from MDPI, Vol.9, No.9, E593, 2019.
(徳島大学機関リポジトリ: 114984,   DOI: 10.3390/ani9090593,   PubMed: 31443357)
18. Namula Zhao, Manita Wittayarat, Maki Hirata, Hirano Takayuki, Nguyen Thi Nhien, Le Anh Quynh, Fahrudin Mokhamad, Fuminori Tanihara and Takeshige Otoi :
Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.8, 598-603, 2019.
(DOI: 10.1007/s11626-019-00385-w,   PubMed: 31297696)
19. Fuminori Tanihara, Maki Hirata, Morikawa Shigeki, Thi Nhien Nguyen, Le Anh Quynh, Hirano Takayuki, Fukumi Yoshiyuki, Abe Toshiaki and Takeshige Otoi :
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts,
The Journal of Reproduction and Development, Vol.65, No.5, 475-479, 2019.
(徳島大学機関リポジトリ: 115540,   DOI: 10.1262/jrd.2019-049,   PubMed: 31178553)
20. Fuminori Tanihara, Maki Hirata, Satoru Iizuka, Shinya Sairiki, Masahiro Nii, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
Relationship among ovarian follicular status, developmental competence of oocytes, and anti-M llerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.,
Animal Science Journal, Vol.90, No.6, 712-718, 2019.
(DOI: 10.1111/asj.13200,   PubMed: 30977253)
21. Nhien Nguyen Thi, Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.54, No.5, 750-755, 2019.
(DOI: 10.1111/rda.13417,   PubMed: 30788874,   Elsevier: Scopus)
22. Zhao Namula, Fuminori Tanihara, Manita Wittayarat, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.,
Acta Veterinaria Hungarica, Vol.67, No.1, 106-114, 2019.
(DOI: 10.1556/004.2019.012,   PubMed: 30922097)
23. Maki Hirata, Fuminori Tanihara, Manita Wittayarat, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, Zhao Namula, Masahiro Nii and Takeshige Otoi :
Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.4, 237-242, 2019.
(DOI: 10.1007/s11626-019-00338-3,   PubMed: 30820813)
24. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh LE, Takayuki Hirano and Takeshige Otoi :
Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos.,
The Journal of Reproduction and Development, Vol.65, No.3, 209-214, 2019.
(徳島大学機関リポジトリ: 115541,   DOI: 10.1262/jrd.2018-116,   PubMed: 30726783,   Elsevier: Scopus)
25. Fuminori Tanihara, Maki Hirata, Nhien T. Nguyen, Quynh A. Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.,
Animal Science Journal, Vol.90, No.1, 55-61, 2018.
(DOI: 10.1111/asj.13129,   PubMed: 30368976)
26. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.,
PLoS ONE, Vol.13, No.10, e0206360, 2018.
(徳島大学機関リポジトリ: 112917,   DOI: 10.1371/journal.pone.0206360,   PubMed: 30352075)
27. Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Mitsuhiro Takagi, Tsukasa Terazono and Takeshige Otoi :
Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.,
Veterinary Medicine and Science, 2018.
(DOI: 10.1002/vms3.121,   PubMed: 30273973)
28. Maki Hirata, Fuminori Tanihara, Taniguchi Masayasu, Takagi Mitsuhiro, Terazono Tsukasa and Takeshige Otoi :
Viability of canine ovaries autografted to different peripheral sites,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, No.2, 140-148, 2018.
29. Zhao Namula, Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Nhien Nguyen Thi, Takayuki Hirano, Masahiro Nii and Takeshige Otoi :
Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.,
Reproduction in Domestic Animals = Zuchthygiene, 2018.
(DOI: 10.1111/rda.13288,   PubMed: 30053311)
30. Tetsushi Ono, Mitsuhiro Takagi, Chiho Kawashima, B Missaka P Wijayagunawardane, M Peter L A Vos, Masayasu Taniguchi, Fuminori Tanihara and Takeshige Otoi :
Comparative Effects of Different Dosages of hCG on Follicular Development in Postpartum Dairy Cows With Cystic Ovarian Follicles.,
Frontiers in Veterinary Science, Vol.5, 2018.
(徳島大学機関リポジトリ: 114493,   DOI: 10.3389/fvets.2018.00130,   PubMed: 30009161)
31. Thanh-Van Nguyen, Manita Wittayarat, Kim Lanh Thi Do, Van Thanh Nguyen, Masahiro Nii, Zhao Namula, Toshiki Kunihara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.,
Animal Science Journal, Vol.89, No.8, 1207-1213, 2018.
(DOI: 10.1111/asj.13049,   PubMed: 29806122)
32. Fuminori Tanihara, Maki Hirata, Thi Nguyen Nhien, Takayuki Hirano, Toshiki Kunihara and Takeshige Otoi :
Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation.,
The Journal of Veterinary Medical Science, Vol.80, No.6, 1007-1011, 2018.
(徳島大学機関リポジトリ: 115574,   DOI: 10.1292/jvms.18-0131,   PubMed: 29769451)
33. V T Nguyen, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Katsutoshi Nishio, T Do L Kim, V T Nguyen, M Nii and Takeshige Otoi :
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.,
Cryo Letters, Vol.39, No.2, 131-136, 2018.
(PubMed: 29734422,   Elsevier: Scopus)
34. Katsutoshi Nishio, Fuminori Tanihara, T-V Nguyen, Toshiki Kunihara, M Nii, Maki Hirata, Tatsuya Takemoto and Takeshige Otoi :
Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.53, No.2, 313-318, 2017.
(DOI: 10.1111/rda.13106,   PubMed: 29135047)
35. T-V Nguyen, Fuminori Tanihara, Ltk Do, Y Sato, M Taniguchi, M Takagi, T Nguyen Van and Takeshige Otoi :
Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.52, No.6, 969-975, 2017.
(DOI: 10.1111/rda.13005,   PubMed: 28660662)
36. Katsutoshi Nishio, Mado Yamazaki, Masayasu Taniguchi, Kazuhiko Besshi, Fumio Morita, Toshiki Kunihara, Fuminori Tanihara, Tatsuya Takemoto and Takeshige Otoi :
Sensitivity of the meiotic stage to hyperthermia during in vitro maturation of porcine oocytes.,
Acta Veterinaria Hungarica, Vol.65, No.1, 115-123, 2017.
(DOI: 10.1556/004.2017.012,   PubMed: 28244334)
37. Fuminori Tanihara, Tatsuya Takemoto, Eri Kitagawa, Shengbin Rao, Kim Lanh Thi Do, Akira Onishi, Yukiko Yamashita, Chisato Kosugi, Hitomi Suzuki, Shoichiro Sembon, Shunichi Suzuki, Michiko Nakai, Masakazu Hashimoto, Akihiro Yasue, Munehide Matsuhisa, Sumihare Noji, Tatsuya Fujimura, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Somatic cell reprogramming-free generation of genetically modified pigs.,
Science Advances, Vol.2, No.9, 2016.
(徳島大学機関リポジトリ: 110154,   DOI: 10.1126/sciadv.1600803,   PubMed: 27652340)
38. L Do, M Wittayarat, T Terazono, Y Sato, M Taniguchi, Fuminori Tanihara, Tatsuya Takemoto, Y Kazuki, K Kazuki, M Oshimura and Takeshige Otoi :
Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.51, No.6, 1039-1043, 2016.
(DOI: 10.1111/rda.12766)
39. Manita Wittayarat, Yoko Sato, Kim Lanh Thi Do, Kaywalee Chatdarong, Theerawat Tharasanit, Mongkol Techakumphu, Masayasu Taniguchi and Takeshige Otoi :
Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.,
Animal Science Journal, 2016.
(DOI: 10.1111/asj.12676,   PubMed: 27506538,   Elsevier: Scopus)
40. N Kurniani Karja Wayan, M Fahrudin, MA Setiadi, LI Tumbelaka, R Sudarwati, YT Hastuti, BH Mulia, A Widianti, K Sultan, T Terazono, Z Namula, M Taniguchi, Fuminori Tanihara, Tatsuya Takemoto, K Kikuchi, Y Sato and Takeshige Otoi :
Characteristics and fertility of sumatran tiger spermatozoa cryopreserved with different sugars.,
Cryo Letters, Vol.37, No.4, 264-271, 2016.
(PubMed: 27925009,   Elsevier: Scopus)
41. Kyohei Kuse, Jumpei Ito, Ariko Miyake, Junna Kawasaki, Shinya Watanabe, Isaac Makundi, Ha Minh Ngo, Takeshige Otoi and Kazuo Nishigaki :
Existence of two distinct infectious ERVs in domestic cats and their different strategies for adaptation to transcriptional regulation.,
Journal of Virology, 2016.
(DOI: 10.1128/JVI.00716-16,   PubMed: 27466428)
42. Morita Yasuhiro, Taniguchi Masayasu, Fuminori Tanihara, Ito Aya, Namula Zhao, DO Thi Kim Lanh, Takagi Mitsuhiro, Tatsuya Takemoto and Takeshige Otoi :
The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture,
The Journal of Veterinary Medical Science, 2016.
(徳島大学機関リポジトリ: 115575,   DOI: 10.1292/jvms.15-0658,   PubMed: 26947170)
43. Tetsushi Ono, Asako Takaoka, Yasuhiro Morita, Lanh Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus-oocyte complexes.,
Acta Veterinaria Hungarica, Vol.63, No.4, 485-498, 2015.
(DOI: 10.1556/004.2015.045,   PubMed: 26599095,   Elsevier: Scopus)
44. L T. K. Do, Y Shibata, M Taniguchi, M Nii, T V. Nguyen, Fuminori Tanihara, M Takagi and Takeshige Otoi :
Melatonin Supplementation During In Vitro Maturation and Development Supports the Development of Porcine Embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.50, No.6, 1054-1058, 2015.
(DOI: 10.1111/rda.12607,   PubMed: 26392209,   Elsevier: Scopus)
45. Y Fushimi, M Takagi, D Monniaux, S Uno, E Kokushi, U Shinya, C Kawashima, Takeshige Otoi, E Deguchi and J Fink-Gremmels :
Effects of Dietary Contamination by Zearalenone and Its Metabolites on Serum Anti-Müllerian Hormone: Impact on the Reproductive Performance of Breeding Cows.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.50, No.5, 834-839, 2015.
(DOI: 10.1111/rda.12599,   PubMed: 26303354,   Elsevier: Scopus)
46. Yasuhiro Morita, Ni Wayan Kurniani Karja, Lanh Thi Kim Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.,
Animal Biotechnology, Vol.26, No.4, 273-275, 2015.
(DOI: 10.1080/10495398.2015.1015681,   PubMed: 26158458,   Elsevier: Scopus)
47. Megumi Shimazaki, Rentsenkhand Sambuu, Yoko Sato, Kim Lanh Thi Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.,
Cryo Letters, Vol.36, No.4, 264-269, 2015.
(PubMed: 26576001,   Elsevier: Scopus)
48. Cut Yasmin, Takeshige Otoi, Mohamad Agus Setiadi and Ni Wayan Kurniani Karja :
Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin.,
Acta Veterinaria Hungarica, Vol.63, No.1, 110-117, 2015.
(DOI: 10.1556/AVet.2015.009,   PubMed: 25655418)
49. Lanh Thi Kim Do, Vien Viet Luu, Yasuhiro Morita, Masayasu Taniguchi, Masahiro Nii, Augustine T. Peter and Takeshige Otoi :
Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes.,
Reproductive Biology, Vol.15, No.2, 86-93, 2015.
(DOI: 10.1016/j.repbio.2015.01.002,   PubMed: 26051456)
50. Tomohiro Isobe, Yoshihisa Ikebata, Lanh Thi Kim Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.,
Animal Science Journal, Vol.86, No.7, 661-665, 2014.
(DOI: 10.1111/asj.12341,   PubMed: 25488699)
51. Masayasu Taniguchi, Manita Wittayarat, Kota Morinaga, Yoko Sato, Lanh Kim Thi, Kaywalee Chatdarong, Mongkol Techakumphu, Masahiro Nii and Takeshige Otoi :
Chelation of trace elements in preservation medium influences the quality of boar spermatozoa during liquid preservation at 5°C for 4 weeks.,
Cryo Letters, Vol.35, No.4, 336-344, 2014.
(PubMed: 25282502)
52. Manita Wittayarat, Akira Fujiwara, Kaywalee Chatdarong, Mongkol Techakumphu, Yoko Sato, Fuminori Tanihara, Yasuhiro Morita, Masayasu Taniguchi and Takeshige Otoi :
Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors.,
Acta Veterinaria Hungarica, Vol.62, No.2, 233-242, 2014.
(DOI: 10.1556/AVet.2013.050,   PubMed: 24334073,   Elsevier: Scopus)
53. Urara Watanabe, Mitsuhiro Takagi, Osamu Yamato, Takeshige Otoi and Koji Okamoto :
Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows.,
Journal of Veterinary Science, Vol.15, No.2, 283-288, 2014.
(DOI: 10.4142/jvs.2014.15.2.283,   PubMed: 24675835)
54. Zhao Namula, Risa Kodama, Fuminori Tanihara, Yasuhiro Morita, Yoko Sato, Manita Wittayarat, Masayasu Taniguchi and Takeshige Otoi :
Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C.,
Acta Veterinaria Hungarica, Vol.62, No.1, 106-116, 2014.
(DOI: 10.1556/AVet.2013.046,   PubMed: 24334075,   Elsevier: Scopus)
55. L T. K. Do, Z Namula, V V. Luu, Y Sato, M Taniguchi, T Isobe, K Kikuchi and Takeshige Otoi :
Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.49, No.2, e17-20, 2014.
(DOI: 10.1111/rda.12274,   PubMed: 24467637)
56. Fuminori Tanihara, Michiko Nakai, Nguyen Thi Men, Noriko Kato, Hiroyuki Kaneko, Junko Noguchi, Takeshige Otoi and Kazuhiro Kikuchi :
Roles of the zona pellucida and functional exposure of the sperm-egg fusion factor 'IZUMO' during in vitro fertilization in pigs.,
Animal Science Journal, Vol.85, No.4, 395-404, 2014.
(DOI: 10.1111/asj.12164,   PubMed: 24450993)
57. Vien Viet Luu, Zhao Namula, Lanh Thi Kim Do, Yoko Sato, Masayasu Taniguchi, Ni Wayan Kurniani Karja and Takeshige Otoi :
Nuclear status and DNA fragmentation of oocytes from porcine, bovine and feline ovaries stored at 4 degrees C for 5 days.,
Cryo Letters, Vol.35, No.1, 48-53, 2014.
(PubMed: 24872157)
58. Fuminori Tanihara, Yukine Kaedei, Zhao Namula, Vien Viet Luu, Yoko Sato, Manita Wittayarat, Masayasu Taniguchi and Takeshige Otoi :
Comparison of activation ability between feline and bovine oocytes.,
Acta Veterinaria Hungarica, Vol.61, No.4, 491-494, 2013.
(DOI: 10.1556/AVet.2013.032,   PubMed: 23974933,   Elsevier: Scopus)
59. Manita Wittayarat, Aya Ito, Taichi Kimura, Zhao Namula, Vien Viet Luu, Lanh Thi Kim Do, Yoko Sato, Masayasu Taniguchi and Takeshige Otoi :
Effects of green tea polyphenol on the quality of canine semen after long-term storage at 5°C.,
Reproductive Biology, Vol.13, No.3, 251-254, 2013.
(DOI: 10.1016/j.repbio.2013.07.006,   PubMed: 24011197)
60. Tomohiro Isobe, Yoshihisa Ikebata, Takeshi Onitsuka, Lanh Thi Kim Do, Yoko Sato, Masayasu Taniguchi and Takeshige Otoi :
Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin.,
Cryobiology, Vol.67, No.2, 184-187, 2013.
(DOI: 10.1016/j.cryobiol.2013.06.010,   PubMed: 23850826)
61. Manita Wittayarat, Yoko Sato, Lanh Thi Kim Do, Yasuhiro Morita, Kaywalee Chatdarong, Mongkol Techakumphu, Masayasu Taniguchi and Takeshige Otoi :
Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos.,
Cellular Reprogramming, Vol.15, No.4, 301-308, 2013.
(DOI: 10.1089/cell.2012.0094,   PubMed: 23790014)
62. Fuminori Tanihara, Michiko Nakai, Hiroyuki Kaneko, Junko Noguchi, Takeshige Otoi and Kazuhiro Kikuchi :
Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.,
The Journal of Reproduction and Development, Vol.59, No.4, 385-392, 2013.
(DOI: 10.1262/jrd.2013-021,   PubMed: 23666494,   Elsevier: Scopus)
63. Yoko Sato, Kaoru Yoshida, Shiari Nozawa, Miki Yoshiike, Michiko Arai, Takeshige Otoi and Teruaki Iwamoto :
Establishment of adult mouse Sertoli cell lines by using the starvation method.,
Reproduction, Vol.145, No.5, 505-516, 2013.
(DOI: 10.1530/REP-12-0086,   PubMed: 23509371)
64. Vien Viet Luu, Keisuke Hanatate, Fuminori Tanihara, Yoko Sato, Lanh Thi Kim Do, Masayasu Taniguchi and Takeshige Otoi :
The effect of relaxin supplementation of in vitro maturation medium on the development of cat oocytes obtained from ovaries stored at 4°C.,
Reproductive Biology, Vol.13, No.2, 122-126, 2013.
(DOI: 10.1016/j.repbio.2013.04.002,   PubMed: 23719116,   Elsevier: Scopus)
65. Masahito Komuro, Kenichi Suzuki, Makoto Kanebako, Takashi Kawahara, Takeshige Otoi, Kenji Kitazato, Toshio Inagi, Kimiko Makino, Masakazu Toi and Hiroshi Terada :
Novel iontophoretic administration method for local therapy of breast cancer.,
Journal of Controlled Release, Vol.168, No.3, 298-306, 2013.
(DOI: 10.1016/j.jconrel.2013.03.021,   PubMed: 23562634)
66. Zhao Namula, Yoko Sato, Risa Kodama, Kouta Morinaga, Viet Vien Luu, Masayasu Taniguchi, Michiko Nakai, Fuminori Tanihara, Kazuhiro Kikuchi, Takashi Nagai and Takeshige Otoi :
Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks.,
Animal Science Journal, Vol.84, No.8, 600-606, 2013.
(DOI: 10.1111/asj.12049,   PubMed: 23607795,   Elsevier: Scopus)
67. Budiyanto Agung, Takeshige Otoi, Dai-ichiro Fuchimoto, Shoichiro Senbon, Akira Onishi and Takashi Nagai :
In vitro fertilization and development of porcine oocytes matured in follicular fluid.,
The Journal of Reproduction and Development, Vol.59, No.2, 103-106, 2013.
(DOI: 10.1262/jrd.2011-050,   PubMed: 23428620)
68. Urara Watanabe, Koji Okamoto, Akio Miyamoto, Takeshige Otoi, Osamu Yamato, Chenga Tshering and Mitsuhiro Takagi :
Japanese Black breeding herd exhibiting low blood urea nitrogen: a metabolic profile study examining the effect on reproductive performance.,
Animal Science Journal, Vol.84, No.5, 389-394, 2012.
(DOI: 10.1111/asj.12012,   PubMed: 23607460)
69. Urara Watanabe, Mitsuhiro Takagi, Osamu Yamato, Takeshige Otoi, Chenga Tshering and Koji Okamoto :
Metabolic profile of Japanese Black breeding cattle herds: usefulness in selection for nutrient supplementation to enhance reproductive performance and regional differences.,
The Journal of Veterinary Medical Science, Vol.75, No.4, 481-487, 2012.
(DOI: 10.1292/jvms.12-0441,   PubMed: 23196825)
70. M Wittayarat, A Thongphakdee, K Saikhun, K Chatdarong, Takeshige Otoi and M Techakumphu :
Cell cycle synchronization of skin fibroblast cells in four species of family Felidae.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.48, No.2, 305-310, 2012.
(DOI: 10.1111/j.1439-0531.2012.02149.x,   PubMed: 22834557)
71. Rentsenkhand Sambuu, Mitsuhiro Takagi, Zhao Namula, Masahiro Nii, Masayasu Taniguchi, Seiich Uno, Emiko Kokushi, Chenga Tshering, Regiane Santos Rodrigues dos, Johanna Fink-Gremmels and Takeshige Otoi :
Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium.,
Animal Science Journal, Vol.84, No.1, 28-34, 2012.
(DOI: 10.1111/j.1740-0929.2012.01033.x,   PubMed: 23302079)
72. T Isobe, Y Ikebata, T Onitsuka, M Wittayarat, Y Sato, M Taniguchi and Takeshige Otoi :
Effect of sericin on preimplantation development of bovine embryos cultured individually.,
Theriogenology, Vol.78, No.4, 747-752, 2012.
(DOI: 10.1016/j.theriogenology.2012.03.021,   PubMed: 22626775)
73. Y Kaedei, M Naito, H Naoi, Y Sato, M Taniguchi, Fuminori Tanihara, K Kikuchi, T Nagai and Takeshige Otoi :
Effects of (-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.47, No.6, 880-886, 2012.
(DOI: 10.1111/j.1439-0531.2012.01984.x,   PubMed: 22299777,   Elsevier: Scopus)
74. Yoko Sato, Shiari Nozawa, Miki Yoshiike, Takeshige Otoi and Teruaki Iwamoto :
Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis.,
Human Reproduction, Vol.27, No.3, 659-668, 2012.
(DOI: 10.1093/humrep/der430,   PubMed: 22215623)
75. Manita Wittayarat, Taichi Kimura, Risa Kodama, Zhao Namula, Kaywalee Chatdarong, Mongkol Techakumphu, Yoko Sato, Masayasu Taniguchi and Takeshige Otoi :
Long-term preservation of chilled canine semen using vitamin C in combination with green tea polyphenol.,
Cryo Letters, Vol.33, No.4, 318-326, 2012.
(PubMed: 22987243)
76. Pimprapar Wongsrikeao, Dyana Saenz, Tommy Rinkoski, Takeshige Otoi and Eric Poeschla :
Antiviral restriction factor transgenesis in the domestic cat.,
Nature Methods, Vol.8, No.10, 853-859, 2011.
(DOI: 10.1038/nmeth.1703,   PubMed: 21909101)
77. Masayasu Taniguchi, Rie Arikawa, Yukine Kaedei, Fuminori Tanihara, Zhao Namula, Vien Luu Viet, Yoko Sato and Takeshige Otoi :
Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes.,
Cryo Letters, Vol.32, No.5, 410-414, 2011.
(PubMed: 22020463,   Elsevier: Scopus)
78. T Terazono, Y Kaedei, Fuminori Tanihara, Z Namula, Vl Viet, M Takagi, M Inoue, Y Sato, M Taniguchi and Takeshige Otoi :
Follicle formation in the canine ovary after autografting to a peripheral site.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.47, No.2, e16-21, 2011.
(DOI: 10.1111/j.1439-0531.2011.01880.x,   PubMed: 21883513,   Elsevier: Scopus)
79. T Terazono, M Inoue, Y Kaedei, Fuminori Tanihara, Z Namula, L V Viet, Y Taura, M Takagi, T Takuma and Takeshige Otoi :
Assessment of canine ovaries autografted to various body sites.,
Theriogenology, Vol.77, No.1, 131-138, 2011.
(DOI: 10.1016/j.theriogenology.2011.07.026,   PubMed: 21872320,   Elsevier: Scopus)
80. Masao Murakami, Juan Ya Dong, Tatsuyuki Suzuki, Masayasu Taniguchi, Yukine Kaedei, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media.,
Cryobiology, Vol.63, No.3, 170-174, 2011.
(DOI: 10.1016/j.cryobiol.2011.06.002,   PubMed: 21893054,   Elsevier: Scopus)
81. Rentsenkhand Sambuu, Mitsuhiro Takagi, Zhao Namula, Takeshige Otoi, Satoshi Shiga, Regiane Dos Santos Rodrigues and Johanna Fink-Gremmels :
Effects of exposure to zearalenone on porcine oocytes and sperm during maturation and fertilization in vitro.,
The Journal of Reproduction and Development, Vol.57, No.4, 547-550, 2011.
(DOI: 10.1262/jrd.11-026H,   PubMed: 21606630)
82. Atsushi Taniyama, Yasutaka Watanabe, Youji Nishino, Tetsurou Inoue, Yasuho Taura, Mitsuhiro Takagi, Chikara Kubota and Takeshige Otoi :
Assisted hatching of poor-quality bovine embryos increases pregnancy success rate after embryo transfer.,
The Journal of Reproduction and Development, Vol.57, No.4, 543-546, 2011.
(DOI: 10.1262/jrd.10-096T,   PubMed: 21606632)
83. Pimprapar Wongsrikeao, Shizuyo Sutou, Miho Kunishi, Ya Juan Dong, Xuejin Bai and Takeshige Otoi :
Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter.,
Prion, Vol.5, No.1, 39-46, 2011.
(DOI: 10.4161/pri.5.1.14075,   PubMed: 21084838)
84. Rentsenkhand Sambuu, Mitsuhiro Takagi, Satoshi Shiga, Seiichi Uno, Emiko Kokushi, Zhao Namula, Takeshige Otoi, Akio Miyamoto, Eisaburo Deguchi and Johanna Fink-Gremmels :
Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry.,
The Journal of Reproduction and Development, Vol.57, No.2, 303-306, 2010.
(DOI: 10.1262/jrd.10-106M,   PubMed: 21139326)
85. V E Abakushina, Y Morita, Y Kaedei, Fuminori Tanihara, Z Namula, L V Viet and Takeshige Otoi :
Formation of an antral follicle-like structure of bovine cumulus-oocyte complexes embedded individually or in groups in collagen gels.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.46, No.3, 423-427, 2010.
(DOI: 10.1111/j.1439-0531.2010.01684.x,   PubMed: 20723136,   Elsevier: Scopus)
86. Yavari Morteza, Naoi Hideaki, Kaedei Yukine, Fuminori Tanihara, Namula Zhao, Viet Luu Vien and Takeshige Otoi :
Effects of epigallocatechin- 3-gallate on the developmental competence of parthenogenetic embryos in the pig,
Italian Journal of Animal Science, Vol.9, No.4, 386-389, 2010.
(DOI: 10.4081/ijas.2010.e73)
87. Makoto Seki, Norio Watanabe, Kenyo Ishii, Yoh-Ichi Kinoshita, Takehiro Aihara, Shuji Takeiri and Takeshige Otoi :
Plasma progesterone profiles in Beagle bitches with and without the whelping experience.,
Acta Veterinaria Hungarica, Vol.58, No.1, 117-124, 2010.
(DOI: 10.1556/AVet.58.2010.1.12,   PubMed: 20159745)
88. B Agung, Y Piao, D Fuchimoto, S Senbon, A Onishi, Takeshige Otoi and T Nagai :
Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid.,
Theriogenology, Vol.73, No.7, 893-899, 2010.
(DOI: 10.1016/j.theriogenology.2009.11.013,   PubMed: 20060577)
89. Makoto Seki, Norio Watanabe, Kenyo Ishii, Yoh-ichi Kinoshita, Takehiro Aihara, Shuji Takeiri and Takeshige Otoi :
Influence of parity and litter size on gestation length in beagle dogs.,
Canadian Journal of Veterinary Research, Vol.74, No.1, 78-80, 2010.
(PubMed: 20357965)
90. Kaedei Yukine, Fujiwara Akira, Ito Aya, Fuminori Tanihara, Morita Yasuhiro, Hanatate Keisuke, Viet Luu Vien, Namula Zhao and Takeshige Otoi :
Effect of Roscovitine Pretreatment on the Meiotic Maturation of Bovine Oocytes and their Subsequent Development after Somatic Cell Nuclear Transfer,
Journal of Animal and Veterinary Advances, Vol.9, No.22, 2848-2853, 2010.
(DOI: 10.3923/javaa.2010.2848.2853,   Elsevier: Scopus)
91. Y Kaedei, A Fujiwara, Fuminori Tanihara, Z Namula, VL Viet and Takeshige Otoi :
In vitro development of cat interspecies nuclear transfer using pig's and cow's cytoplasm,
Bulletin of the Veterinary Institute in Pulawy, Vol.54, No.3, 405-408, 2010.
(Elsevier: Scopus)
92. Tetsuya Takuma, Sayoko Sakai, Daisuke Ezoe, Hitoshi Ichimaru, Takaomi Jinnouchi, Yukine Kaedei, Takashi Nagai and Takeshige Otoi :
Effects of season and reproductive phase on the quality, quantity and developmental competence of oocytes aspirated from Japanese black cows.,
The Journal of Reproduction and Development, Vol.56, No.1, 55-59, 2009.
(DOI: 10.1262/jrd.09-071H,   PubMed: 19815989)
93. M Hayashi, H Tsuchiya, Takeshige Otoi, B Agung, N Yamamoto and K Tomita :
Influence of freezing with liquid nitrogen on whole-knee joint grafts and protection of cartilage from cryoinjury in rabbits.,
Cryobiology, Vol.59, No.1, 28-35, 2009.
(DOI: 10.1016/j.cryobiol.2009.04.002,   PubMed: 19362085)
94. A Fujii, Y Kaedei, Fuminori Tanihara, A Ito, K Hanatate, K Kikuchi, T Nagai and Takeshige Otoi :
In vitro maturation and development of porcine oocytes cultured in a straw or dish using a portable incubator with a CO2 chamber.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.45, No.4, 619-624, 2009.
(DOI: 10.1111/j.1439-0531.2008.01316.x,   PubMed: 19144033,   Elsevier: Scopus)
95. T Takuma, T Otsubo, Y Kurokawa, H Ichimaru and Takeshige Otoi :
Effects of twice-weekly follicular punctures of ovaries with or without the corpus luteum on follicular and luteal dynamics.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.45, No.1, 50-54, 2008.
(DOI: 10.1111/j.1439-0531.2008.01224.x,   PubMed: 19144025)
96. Mitsuhiro Takagi, Shuhei Mukai, Toshiyuki Kuriyagawa, Katsuhito Takagaki, Seiichi Uno, Emiko Kokushi, Takeshige Otoi, Agung Budiyanto, Koumei Shirasuna, Akio Miyamoto, Osamu Kawamura, Koji Okamoto and Eisaburo Deguchi :
Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle.,
Reproductive Toxicology, Vol.26, No.2, 164-169, 2008.
(DOI: 10.1016/j.reprotox.2008.08.006,   PubMed: 18790045)
97. H Naoi, B Agung, N W. K. Karja, P Wongsrikeao, R Shimizu, M Taniguchi and Takeshige Otoi :
Effects of the reproductive status on morphological oocyte quality and developmental competence of oocytes after in vitro fertilization and somatic cell nuclear transfer in cat.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.43, No.2, 157-161, 2008.
(DOI: 10.1111/j.1439-0531.2007.00870.x,   PubMed: 18325005)
98. F Barati, B Agung, P Wongsrikeao, M Taniguchi, T Nagai and Takeshige Otoi :
Meiotic competence and DNA damage of porcine oocytes exposed to an elevated temperature.,
Theriogenology, Vol.69, No.6, 767-772, 2008.
(DOI: 10.1016/j.theriogenology.2007.08.038,   PubMed: 18242671)
99. Agung Budiyanto, Barati Farid, Kaedei Yukine, Fuminori Tanihara and Takeshige Otoi :
Meiotic Competence and DNA Damage of Porcine Oocytes from Ovaries Exposed to an Elevated Temperature,
Journal of Animal and Veterinary Advances, Vol.7, No.10, 1179-1183, 2008.
(Elsevier: Scopus)
100. M Takagi, D Yamamoto, S Ogawa, Takeshige Otoi, M Ohtani and A Miyamoto :
Messenger RNA expression of angiotensin-converting enzyme, endothelin, cyclooxygenase-2 and prostaglandin synthases in bovine placentomes during gestation and the postpartum period.,
The Veterinary Journal, Vol.177, No.3, 398-404, 2007.
(DOI: 10.1016/j.tvjl.2007.05.017,   PubMed: 17804266)
101. Ken Maeda, Shigeru Yasumoto, Akari Tsuruda, Kiyohiko Andoh, Kazushige Kai, Takeshige Otoi and Tomio Matsumura :
Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.,
The Journal of Veterinary Medical Science, Vol.69, No.9, 989-991, 2007.
(DOI: 10.1292/jvms.69.989,   PubMed: 17917390)
102. Shizuyo Sutou, Miho Kunishi, Toshiyuki Kudo, Pimprapar Wongsrikeao, Makoto Miyagishi and Takeshige Otoi :
Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology.,
BMC Biotechnology, Vol.7, 44, 2007.
(DOI: 10.1186/1472-6750-7-44,   PubMed: 17655742)
103. Pimprapar Wongsrikeao, Takashi Nagai, Budiyanto Agung, Masayasu Taniguchi, Miho Kunishi, Shizuyo Suto and Takeshige Otoi :
Improvement of transgenic cloning efficiencies by culturing recipient oocytes and donor cells with antioxidant vitamins in cattle.,
Molecular Reproduction and Development, Vol.74, No.6, 694-702, 2007.
(DOI: 10.1002/mrd.20640,   PubMed: 17154297)
104. Masayasu Taniguchi, Akiko Ikeda, Eri Arikawa, Pimprapar Wongsrikeao, Budiyanto Agung, Hideaki Naoi, Takashi Nagai and Takeshige Otoi :
Effect of cryoprotectant composition on in vitro viability of in vitro fertilized and cloned bovine embryos following vitrification and in-straw dilution.,
The Journal of Reproduction and Development, Vol.53, No.4, 963-969, 2007.
(DOI: 10.1262/jrd.18175,   PubMed: 17420619)
105. Takeshige Otoi, T Shin, D C. Kraemer and M E. Westhusin :
Role of cumulus cells on in vitro maturation of canine oocytes.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.42, No.2, 184-189, 2007.
(DOI: 10.1111/j.1439-0531.2006.00750.x,   PubMed: 17348976)
106. Hideaki Naoi, Takeshige Otoi, Takano Shimamura, Ni Wayan Kurniani Karja, Budiyanto Agung, Ryohei Shimizu, Masayasu Taniguchi and Takashi Nagai :
Developmental competence of cat oocytes from ovaries stored at various temperature for 24 h.,
The Journal of Reproduction and Development, Vol.53, No.2, 271-277, 2006.
(DOI: 10.1262/jrd.18115,   PubMed: 17139136)
107. M Taniguchi, A Ikeda, E Arikawa, R Shimizu, M Seki, M Karaki, R Rajamahendran and Takeshige Otoi :
Ovarian follicular and corpus luteum changes, progesterone concentrations, estrus and ovulation following estradiol benzoate/progesterone based treatment protocol in cross-bred cows.,
Animal Reproduction Science, Vol.99, No.3-4, 389-394, 2006.
(DOI: 10.1016/j.anireprosci.2006.08.015,   PubMed: 16989965)
108. Ni Wayan Kurniani Karja, Takeshige Otoi, Pimprapar Wongsrikeao, Ryohei Shimizu, Masako Murakami, Budiyanto Agung, Mokhamad Fahrudin and Takashi Nagai :
Effects of electric field strengths on fusion and in vitro development of domestic cat embryos derived by somatic cell nuclear transfer.,
Theriogenology, Vol.66, No.5, 1237-1242, 2006.
(DOI: 10.1016/j.theriogenology.2006.03.034,   PubMed: 16675008)
109. Takeshige Otoi, R Shimizu, H Naoi, P Wongsrikeao, B Agung and M Taniguchi :
Meiotic competence of canine oocytes embedded in collagen gel.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.41, No.1, 17-21, 2006.
(DOI: 10.1111/j.1439-0531.2006.00639.x,   PubMed: 16420322)
110. Pimprapar Wongsrikeao, Takeshige Otoi, Hirofumi Yamasaki, Budiyanto Agung, Masayasu Taniguchi, Hideaki Naoi, Ryohei Shimizu and Takashi Nagai :
Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos.,
Theriogenology, Vol.66, No.2, 366-372, 2006.
(DOI: 10.1016/j.theriogenology.2005.12.001,   PubMed: 16414109)
111. M Murakami, N W. K. Karja, P Wongsrikeao, B Agung, M Taniguchi, H Naoi and Takeshige Otoi :
Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.40, No.6, 511-515, 2005.
(DOI: 10.1111/j.1439-0531.2005.00617.x,   PubMed: 16324075)
112. Budiyanto Agung, Takeshige Otoi, Pimprapar Wongsrikeao, Masayasu Taniguchi, Ryohei Shimizu, Hajime Watari and Takashi Nagai :
Effect of maturation culture period of oocytes on the sex ratio of in vitro fertilized bovine embryos.,
The Journal of Reproduction and Development, Vol.52, No.1, 123-127, 2005.
(DOI: 10.1262/jrd.17055,   PubMed: 16276038)
113. Ni Wayan Kurniani Karja, Takeshige Otoi, Masako Murakami, Pimprapar Wongsrikeao, Agung Budiyanto, Mokhamad Fahrudin and Takashi Nagai :
Effect of cycloheximide on in vitro development of electrically activated feline oocytes.,
The Journal of Reproduction and Development, Vol.51, No.6, 783-786, 2005.
(DOI: 10.1262/jrd.17056,   PubMed: 16210784)
114. F Rajaei, N W. K. Karja, B Agung, P Wongsrikeao, M Taniguchi, M Murakami, R Sambuu, M Nii and Takeshige Otoi :
Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.40, No.5, 429-432, 2005.
(DOI: 10.1111/j.1439-0531.2005.00585.x,   PubMed: 16149947)
115. Masaomi Mori, Takeshige Otoi, Pimprapar Wongsrikeao, Budiyanto Agung and Takashi Nagai :
Effects of beta-mercaptoethanol and cycloheximide on survival and DNA damage of bovine embryos stored at 4 degrees C for 72 h.,
Theriogenology, Vol.65, No.7, 1322-1332, 2005.
(DOI: 10.1016/j.theriogenology.2005.07.018,   PubMed: 16185762)
116. Ni Wayan Kurniani Karja, Takeshige Otoi, Pimprapar Wongsrikeao, Masako Murakami, Budiyanto Agung, Mokhamad Fahrudin and Takashi Nagai :
In vitro development and post-thaw survival of blastocysts derived from delipidated zygotes from domestic cats.,
Theriogenology, Vol.65, No.2, 415-423, 2005.
(DOI: 10.1016/j.theriogenology.2005.04.029,   PubMed: 15985290)
117. Pimprapar Wongsrikeao, Takeshige Otoi, Masayasu Taniguchi, Ni Wayan Kurniani Karja, Budiyanto Agung, Masaru Nii and Takashi Nagai :
Effects of hexoses on in vitro oocyte maturation and embryo development in pigs.,
Theriogenology, Vol.65, No.2, 332-343, 2005.
(DOI: 10.1016/j.theriogenology.2005.03.027,   PubMed: 15967489)
118. P Wongsrikeao, Y Kaneshige, R Ooki, M Taniguchi, B Agung, M Nii and Takeshige Otoi :
Effect of the removal of cumulus cells on the nuclear maturation, fertilization and development of porcine oocytes.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.40, No.2, 166-170, 2005.
(DOI: 10.1111/j.1439-0531.2005.00576.x,   PubMed: 15819969)
119. B Agung, Takeshige Otoi, H Abe, H Hoshi, M Murakami, N W. K. Karja, M K. Murakami, P Wongsrikeao, H Watari and T Suzuki :
Relationship between oxygen consumption and sex of bovine in vitro fertilized embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.40, No.1, 51-56, 2005.
(DOI: 10.1111/j.1439-0531.2004.00554.x,   PubMed: 15655001)
120. Pimprapar Wongsrikeao, Takeshige Otoi, Ni Wayan Kurniani Karja, Budiyanto Agung, Masahiro Nii and Takashi Nagai :
Effects of ovary storage time and temperature on DNA fragmentation and development of porcine oocytes.,
The Journal of Reproduction and Development, Vol.51, No.1, 87-97, 2005.
(DOI: 10.1262/jrd.51.87,   PubMed: 15750300)
121. Masao Murakami, Takeshige Otoi, Pimprapar Wongsrikeao, Budiyanto Agung, Rentsenkhand Sambuu and Tatsuyuki Suzuki :
Development of interspecies cloned embryos in yak and dog.,
Cloning and Stem Cells, Vol.7, No.2, 77-81, 2005.
(DOI: 10.1089/clo.2005.7.77,   PubMed: 15971981)
122. Ni Wayan Kurniani Karja, Pimprapar Wongsrikeao, Masako Murakami, Budiyanto Agung, Mokhamad Fahrudin, Takashi Nagai and Takeshige Otoi :
Effects of oxygen tension on the development and quality of porcine in vitro fertilized embryos.,
Theriogenology, Vol.62, No.9, 1585-1595, 2004.
(DOI: 10.1016/j.theriogenology.2004.03.012,   PubMed: 15511546)
123. Ni Wayan Kurniani Karja, Sergey Medvedev, Akira Onishi, Dai-Ichiro Fuchimoto, Masaki Iwamoto, Takeshige Otoi and Takashi Nagai :
Effect of replacement of pyruvate/lactate in culture medium with glucose on preimplantation development of porcine embryos in vitro.,
The Journal of Reproduction and Development, Vol.50, No.5, 587-592, 2004.
(DOI: 10.1262/jrd.50.587,   PubMed: 15514466)
124. Mk Murakami, Takeshige Otoi, N W. K. Karja, P Wongsrikeao, B Agung and T Suzuki :
Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose.,
Cryobiology, Vol.48, No.3, 341-348, 2004.
(DOI: 10.1016/j.cryobiol.2004.02.012,   PubMed: 15157782)
125. Pimprapar Wongsrikeao, Takeshige Otoi, Masako Murakami, Ni Wayan Kurniani Karja, Agung Budiyanto, Masao Murakami, Masaru Nii and Tatsuyuki Suzuki :
Relationship between DNA fragmentation and nuclear status of in vitro-matured porcine oocytes: role of cumulus cells.,
Reproduction, Fertility, and Development, Vol.16, No.8, 773-780, 2004.
(DOI: 10.1071/RD03099,   PubMed: 15740700)
126. Takeshige Otoi, Taeyoung Shin, Duane C. Kraemer and Mark E. Westhusin :
Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization.,
Reproduction, Nutrition, Development, Vol.44, No.6, 631-637, 2004.
(DOI: 10.1051/rnd:2004065,   PubMed: 15762307)
127. M Yuge, Takeshige Otoi, M Nii, Mk Murakami, N W. K. Karja, F Rajaei, B Agung, P Wongsrikeao, M Murakami and T Suzuki :
Effects of cooling ovaries before oocyte aspiration on meiotic competence of porcine oocytes and of exposing in vitro matured oocytes to ambient temperature on in vitro fertilization and development of the oocytes.,
Cryobiology, Vol.47, No.2, 102-108, 2003.
(DOI: 10.1016/j.cryobiol.2003.08.001,   PubMed: 14580845)
128. M Murakami, Takeshige Otoi, N W. K. Karja, A Ooka and T Suzuki :
Effects of serum-free culture media on in vitro development of domestic cat embryos following in vitro maturation and fertilization.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.37, No.6, 352-356, 2002.
(DOI: 10.1046/j.1439-0531.2002.00382.x,   PubMed: 12464074)
129. Takeshige Otoi, L Willingham, T Shin, D C. Kraemer and M Westhusin :
Effects of oocyte culture density on meiotic competence of canine oocytes.,
Reproduction, Vol.124, No.6, 775-781, 2002.
(DOI: 10.1530/rep.0.1240775,   PubMed: 12530915)
130. M Fahrudin, Takeshige Otoi, N W. K. Karja, M Mori, M Murakami and T Suzuki :
Analysis of DNA fragmentation in bovine somatic nuclear transfer embryos using TUNEL.,
Reproduction, Vol.124, No.6, 813-819, 2002.
(DOI: 10.1530/rep.0.1240813,   PubMed: 12530919)
131. M Mori, Takeshige Otoi and T Suzuki :
Correlation between the cell number and diameter in bovine embryos produced in vitro.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.37, No.3, 181-184, 2002.
(DOI: 10.1046/j.1439-0531.2002.00354.x,   PubMed: 12071894)
132. N W. K. Karja, Takeshige Otoi, M Murakami, M Fahrudin and T Suzuki :
In vitro maturation, fertilization and development of domestic cat oocytes recovered from ovaries collected at three stages of the reproductive cycle.,
Theriogenology, Vol.57, No.9, 2289-2298, 2002.
(DOI: 10.1016/S0093-691X(02)00905-6,   PubMed: 12141577)
133. N W. Kurniani Karja, Takeshige Otoi, Masako Murakami, Minori Yuge, Mokhamad Fahrudin and Tatsuyuki Suzuki :
Effect of protein supplementation on development to the hatching and hatched blastocyst stages of cat IVF embryos.,
Reproduction, Fertility, and Development, Vol.14, No.5-6, 291-296, 2002.
(DOI: 10.1071/RD01135,   PubMed: 12467353)
134. Y J. Dong, M D. Varisanga, N R. Mtango, M Aono, Takeshige Otoi and T Suzuki :
Improvement of the culture conditions for in vitro production of cattle embryos in a portable CO2 incubator.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.36, No.6, 313-318, 2001.
(DOI: 10.1046/j.1439-0531.2001.00308.x,   PubMed: 11928927)
135. M Fahrudin, Takeshige Otoi and T Suzuki :
Developmental competence of bovine embryos reconstructed by the transfer of somatic cells derived from frozen tissues.,
The Journal of Veterinary Medical Science, Vol.63, No.10, 1151-1154, 2001.
(DOI: 10.1292/jvms.63.1151,   PubMed: 11714036)
136. A S. Abdoon, O M. Kandil, Takeshige Otoi and T Suzuki :
Influence of oocyte quality, culture media and gonadotropins on cleavage rate and development of in vitro fertilized buffalo embryos.,
Animal Reproduction Science, Vol.65, No.3-4, 215-223, 2001.
(DOI: 10.1016/S0378-4320(01)00079-3,   PubMed: 11267801)
137. N R. Mtango, M D. Varisanga, Y J. Dong, Takeshige Otoi and T Suzuki :
The effect of prefreezing the diluent portion of the straw in a step-wise vitrification process using ethylene glycol and polyvinylpyrrolidone to preserve bovine blastocysts.,
Cryobiology, Vol.42, No.2, 135-138, 2001.
(DOI: 10.1006/cryo.2001.2308,   PubMed: 11448115)
138. Takeshige Otoi, M Murakami, A Ooka, N W. Karja and T Suzuki :
Effects of size and storage temperature on meiotic competence of domestic cat oocytes.,
The Veterinary Record, Vol.148, No.4, 116-118, 2001.
(PubMed: 11232928,   Elsevier: Scopus)
139. Takeshige Otoi, A Ooka, M Murakami, N W. Karja and T Suzuki :
Size distribution and meiotic competence of oocytes obtained from bitch ovaries at various stages of the oestrous cycle.,
Reproduction, Fertility, and Development, Vol.13, No.2-3, 151-155, 2001.
(DOI: 10.1071/RD00098,   PubMed: 11720132)
140. Takeshige Otoi, M Fujii, M Tanaka, A Ooka and T Suzuki :
Canine oocyte diameter in relation to meiotic competence and sperm penetration,
Theriogenology, Vol.54, No.4, 535-542, 2000.
(DOI: 10.1016/S0093-691X(00)00368-X,   PubMed: 11071127)
141. Takeshige Otoi, N Koyama, K Yamamoto, N Horikita, S Tachikawa and T Suzuki :
Developmental competence of frozen-thawed blastocysts from fair-quality bovine embryos cultured with beta-mercaptoethanol.,
The Veterinary Journal, Vol.159, No.3, 282-286, 2000.
(DOI: 10.1053/tvjl.1999.0408,   PubMed: 10775474)
142. M Fujii, Takeshige Otoi, M Murakami, M Tanaka, S Une and T Suzuki :
The quality and maturation of bitch oocytes recovered from ovaries by the slicing method.,
The Journal of Veterinary Medical Science, Vol.62, No.3, 305-307, 2000.
(PubMed: 10770604)
143. Takeshige Otoi, M Murakami, M Fujii, M Tanaka, A Ooka, S Une and T Suzuki :
Development of canine oocytes matured and fertilised in vitro.,
The Veterinary Record, Vol.146, No.2, 52-53, 2000.
(PubMed: 10678815)
144. Takeshige Otoi, A SS Abdoon, M TK Omaima, M Tanaka and T Suzuki :
Pellet freezing of in vitro produced bovine embryos using dry ice.,
Cryo Letters, Vol.21, No.1, 31-38, 2000.
(PubMed: 12148062,   Elsevier: Scopus)
145. Takeshige Otoi, K Yamamoto, N Horikita, S Tachikawa and T Suzuki :
Relationship between dead cells and DNA fragmentation in bovine embryos produced in vitro and stored at 4 degrees C.,
Molecular Reproduction and Development, Vol.54, No.4, 342-347, 1999.
(DOI: 10.1002/(SICI)1098-2795(199912)54:4<342::AID-MRD4>3.0.CO;2-7,   PubMed: 10542374)
146. K Yamamoto, Takeshige Otoi, N Koyama, N Horikita, S Tachikawa and T Miyano :
Development to live young from bovine small oocytes after growth, maturation and fertilization in vitro.,
Theriogenology, Vol.52, No.1, 81-89, 1999.
(DOI: 10.1016/S0093-691X(99)00111-9,   PubMed: 10734407)
147. H Abe, Takeshige Otoi, S Tachikawa, S Yamashita, T Satoh and H Hoshi :
Fine structure of bovine morulae and blastocysts in vivo and in vitro.,
Anatomy and Embryology, Vol.199, No.6, 519-527, 1999.
(DOI: 10.1007/s004290050249,   PubMed: 10350132,   Elsevier: Scopus)
148. Takeshige Otoi, M Fujii, M Tanaka, A Ooka and T Suzuki :
Effect of serum on the in vitro maturation of canine oocytes.,
Reproduction, Fertility, and Development, Vol.11, No.7-8, 387-390, 1999.
(PubMed: 11101272)
149. Takeshige Otoi, K Yamamoto, N Koyama, S Tachikawa and T Suzuki :
Cryopreservation of mature bovine oocytes by vitrification in straws.,
Cryobiology, Vol.37, No.1, 77-85, 1998.
(DOI: 10.1006/cryo.1998.2103,   PubMed: 9698432)
150. Takeshige Otoi, N Koyama, K Yamamoto, S Tachikawa and T Suzuki :
Superovulatory responses in Japanese black beef cows following largest follicle aspiration or human chorionic gonadotrophin (hCG) treatment.,
The Journal of Veterinary Medical Science, Vol.60, No.8, 961-963, 1998.
(PubMed: 9764411)
151. M Murakami, Takeshige Otoi, C Sumantri and T Suzuki :
Effects of centrifugation and lipid removal on the cryopreservation of in vitro produced bovine embryos at the eight-cell stage.,
Cryobiology, Vol.36, No.3, 206-212, 1998.
(DOI: 10.1006/cryo.1998.2077,   PubMed: 9597740)
152. Takeshige Otoi, N Koyama, K Yamamoto and S Tachikawa :
Superovulatory response in beef cows following removal of the largest ovarian follicle.,
The Veterinary Record, Vol.142, No.15, 402-403, 1998.
(PubMed: 9586134)
153. Takeshige Otoi, K Yamamoto, N Koyama, S Tachikawa and T Suzuki :
Bovine oocyte diameter in relation to developmental competence.,
Theriogenology, Vol.48, No.5, 769-774, 1997.
(DOI: 10.1016/S0093-691X(97)00300-2,   PubMed: 16728170)
154. Takeshige Otoi, K Yamamoto, N Koyama, S Tachikawa, M Murakami, Y Kikkawa and T Suzuki :
Cryopreservation of mature bovine oocytes following centrifugation treatment.,
Cryobiology, Vol.34, No.1, 36-41, 1997.
(DOI: 10.1006/cryo.1996.1988,   PubMed: 9028915)
155. Takeshige Otoi, K Yamamoto, N Koyama and S Tachikawa :
Development of oocytes derived from the first dominant follicles of beef cows.,
The Veterinary Record, Vol.139, No.16, 396, 1996.
(PubMed: 8913014)
156. Takeshige Otoi, K Yamamoto, N Koyama, S Tachikawa and T Suzuki :
A frozen-thawed in vitro-matured bovine oocyte derived calf with normal growth and fertility.,
The Journal of Veterinary Medical Science, Vol.58, No.8, 811-813, 1996.
(PubMed: 8877988)
157. S Saha, Takeshige Otoi, M Takagi, A Boediono, C Sumantri and T Suzuki :
Normal calves obtained after direct transfer of vitrified bovine embryos using ethylene glycol, trehalose, and polyvinylpyrrolidone.,
Cryobiology, Vol.33, No.3, 291-299, 1996.
(DOI: 10.1006/cryo.1996.0029,   PubMed: 8689886)
158. Takeshige Otoi, K Yamamoto, N Koyama and T Suzuki :
In vitro fertilization and development of immature and mature bovine oocytes cryopreserved by ethylene glycol with sucrose.,
Cryobiology, Vol.32, No.5, 455-460, 1995.
(DOI: 10.1006/cryo.1995.1045,   PubMed: 7587284)
159. Takeshige Otoi, S Tachikawa, S Kondo, M Takagi and T Suzuki :
Developmental competence of bovine oocytes frozen at different cooling rates.,
Cryobiology, Vol.31, No.4, 344-348, 1994.
(DOI: 10.1006/cryo.1994.1041,   PubMed: 7924393)
160. M Takagi, L Sakonju, Takeshige Otoi, K Hamana and T Suzuki :
Postthaw viability of the inner cell mass of in vitro-matured/in vitro-fertilized bovine embryos frozen in various cryoprotectants.,
Cryobiology, Vol.31, No.4, 398-405, 1994.
(DOI: 10.1006/cryo.1994.1048,   PubMed: 7924397)
161. M Takagi, Takeshige Otoi, A Boediono, S Saha and T Suzuki :
Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.,
Theriogenology, Vol.41, No.4, 915-921, 1994.
(DOI: 10.1016/0093-691X(94)90507-F,   PubMed: 16727445)
162. Takeshige Otoi, S Tachikawa, S Kondo and T Suzuki :
Effect of washing, antibiotics and trypsin treatment of bovine embryos on the removal of adhering K99+ Escherichia coli.,
The Journal of Veterinary Medical Science, Vol.55, No.6, 1053-1055, 1993.
(PubMed: 8117806)
163. M Takagi, Takeshige Otoi and T Suzuki :
Survival rate of frozen-thawed bovine IVM/IVF embryos in relation to post-thaw exposure time in two cryoprotectants.,
Cryobiology, Vol.30, No.5, 466-469, 1993.
(DOI: 10.1006/cryo.1993.1047,   PubMed: 8252914)
164. Takeshige Otoi, S Tachikawa, S Kondo and T Suzuki :
Developmental capacity of bovine oocytes frozen in different cryoprotectants.,
Theriogenology, Vol.40, No.4, 801-807, 1993.
(DOI: 10.1016/0093-691X(93)90215-Q,   PubMed: 16727361)
165. Takeshige Otoi, S Tachikawa, S Kondo, F Kono, M Goto, Y Ogano and T Suzuki :
Effect of trypsin treatment of in vitro fertilized bovine embryos on their subsequent survival and development.,
The Journal of Veterinary Medical Science, Vol.55, No.2, 237-239, 1993.
(PubMed: 8513003)
166. S Tachikawa, Takeshige Otoi, S Kondo, T Machida and M Kasai :
Successful vitrification of bovine blastocysts, derived by in vitro maturation and fertilization.,
Molecular Reproduction and Development, Vol.34, No.3, 266-271, 1993.
(DOI: 10.1002/mrd.1080340306,   PubMed: 8471248)
167. Takeshige Otoi, S Tachikawa, S Kondo and T Suzuki :
Effects of different lots of semen from the same bull on in vitro development of bovine oocytes fertilized in vitro.,
Theriogenology, Vol.39, No.3, 713-718, 1993.
(DOI: 10.1016/0093-691X(93)90256-5,   PubMed: 16727248)
168. Takeshige Otoi, S Tachikawa, S Kondo and T Suzuki :
Developmental capacity of bovine oocytes cryopreserved after maturation in vitro and of frozen-thawed bovine embryos derived from frozen mature oocytes.,
Theriogenology, Vol.38, No.4, 711-719, 1992.
(DOI: 10.1016/0093-691X(92)90033-N,   PubMed: 16727173)
169. Takeshige Otoi, S Tachikawa, S Kondo and T Suzuki :
Effect of antibiotics treatment of in vitro fertilized bovine embryos to remove adhering bacteria.,
The Journal of Veterinary Medical Science, Vol.54, No.4, 763-765, 1992.
(PubMed: 1391188)

国際会議:

1. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
2. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Hirano Takayuki, Le Anh Quynh and Takeshige Otoi :
Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
3. Le Anh Quynh, Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Hirano Takayuki and Takeshige Otoi :
Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
4. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
5. Katsutoshi Nishio, Fuminori Tanihara, TV Nguyen, Toshiki Kunihara and Takeshige Otoi :
Effects of voltage strength on development and quality of electroporated porcine embryos.,
Transgenic Research, Vol.26, No.1, 29, Utah, USA, Oct. 2017.
6. Fuminori Tanihara, LTK Do, TV Nguyen, Toshiki Kunihara, Katsutoshi Nishio, Tatsuya Takemoto and Takeshige Otoi :
Generation of TP53-modified pigs by GEEP method: CRISPR/Cas9-mediated gene modification introduced into porcine zygotes by electroporation.,
Transgenic Research, Vol.26, No.1, 38, Utah, USA, Oct. 2017.
7. Fuminori Tanihara and Takeshige Otoi :
A simple-step generation of genetically modified pigs by genome editing by electroporation of Cas9 protein (GEEP) method.,
Forth World Congress of Reproductive Bioligy (WCRB2017), Okinawa, Sep. 2017.
8. R Kuriwaki, Y Sato, S Hagino, M Shimazaki, R Sambuu, LTK Do, Fuminori Tanihara, M Takagi, M Taniguchi and Takeshige Otoi :
Evaluation of the Leydig cell function on crossbreeding yak showing infertility.,
Forth World Congress of Reproductive Bioligy (WCRB2017), Okinawa, Sep. 2017.

国内講演発表:

1. 谷原 史倫, 平田 真樹, Nguyen Thi Nhien, Quynh Anh Le, 平野 隆之, 音井 威重 :
エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製,
第162回日本獣医学会学術集会, 2019年9月.
2. 平田 真樹, 谷原 史倫, NGUYEN NHIEN THI, Namula Zhao, 音井 威重 :
ブタ体外受精卵におけるCrispr/Cas9システムを使用したゲノム編集の効率,
日本ゲノム編集学会 第3回大会, 2018年6月.
3. 谷原 史倫, 平田 真樹, NGUYEN NHIEN THI, 平野 隆之, 音井 威重 :
ブタ内在性レトロウイルス遺伝子を標的としたゲノム編集が胚発育能に及ぼす影響,
日本ゲノム編集学会第3回大会, 2018年6月.

その他・研究会:

1. 谷原 史倫, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 :
CRISPR/Cas9システムによるブタ体外受精卵のINS遺伝子への点変異導入,
第7回日本先進医工学ブタ研究会, 2019年10月.
2. 音井 威重, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 谷原 史倫 :
GEEP法を用いた遺伝子改変ブタの作製と遺伝子改変効率,
第7回日本先進医工学ブタ研究会, 2019年10月.
3. 平田 真樹, 谷原 史倫, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 :
ブタ体外受精卵におけるCRISPR/Cas9システムを用いた複数遺伝子の同時改変,
第7回日本先進医工学ブタ研究会, 2019年10月.
4. Le Anh Quynh, Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano and Takeshige Otoi :
Concentration of CRISPR/Cas9 components effects on genetic mosaicism of cytoplasmic microinjected porcine embryos,
第6回日本先進医工学ブタ研究会, Oct. 2018.
5. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Le Anh Quynh, 新居 雅宏 and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid,
第6回日本先進医工学ブタ研究会, Oct. 2018.
6. 平田 真樹, 谷原 史倫, 平野 隆之, NGUYEN NHIEN THI, LE ANH QUYNH, 新居 雅宏, 音井 威重 :
ブタにおける受精前後でのゲノム編集が胚盤胞の変異導入効率に及ぼす影響,
第6回日本先進医工学ブタ研究会, 2018年10月.
7. 谷原 史倫, 平田 真樹, NGUYEN NHIEN THI, LE ANH QUYNH, 平野 隆之, 竹本 龍也, 中井 美智子, 淵本 大一郎, 音井 威重 :
ゲノム編集によるTP53遺伝子改変ブタの作製と表現型の解析,
第6回日本先進医工学ブタ研究会, 2018年10月.
8. Fuminori Tanihara, Tatsuya Takemoto, Maki Hirata, N Nguyen Thi, Toshiki Kunihara, R Nishinakamura and Takeshige Otoi :
Modification of SALL1 gene via CRISPR/Cas9-mediated gene editing introduced into porcine zygotes by electroporation,
KEY Forum: The 3rd International symposium on Stem Cell Traits and Developmental Systems, Jan. 2018.
9. 谷原 史倫, 音井 威重 :
ゲノム編集技術を用いたブタでの応用例,
第3回日本生物工学会西日本支部講演会「ゲノム編集—多様な生物種への応用研究」, 2016年12月.
10. 谷原 史倫, 竹本 龍也, 中井 美智子, 北川 絵理, DO THI KIM LANH, 大西 彰, 鈴木 俊一, 千本 正一郎, 淵本 大一郎, 音井 威重 :
新規ゲノム編集技術を用いたPDX-1遺伝子改変ブタの作製,
第4回 日本先進医工学ブタ研究会 要旨集, 21, 2016年10月.

報告書:

1. 植野 美彦, 澤田 麻衣子, 荒武 達朗, 橋本 一郎, 二川 健, 安井 敏之, 浜田 賢一, 白山 靖彦, 山田 健一, 北條 昌秀, 音井 威重, 古屋 S. 玲, 関 陽介 :
平成29年度 徳島大学総合教育センターアドミッション部門 報告書,
平成29年度 徳島大学総合教育センターアドミッション部門 報告書, 2018年3月.

特許:

1. 竹本 龍也, 橋本 昌和, 音井 威重, 谷原 史倫 : 特願2016-074645 (2016年4月), 特許第2016-074645号 (2016年4月).

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

  • ゲノム編集技術を用いた免疫不全マイクロミニブタの創出 (研究課題/領域番号: 18K12062 )
  • 内在性レトロウイルス感染制御による安全な異種移植用ブタの作製 (研究課題/領域番号: 17K19325 )
  • 染色体工学技術とゲノム編集技術の融合による遺伝子改変ブタの作製 (研究課題/領域番号: 17H03938 )
  • アジアゾウにおける精子成熟因子の調査研究 (研究課題/領域番号: 15H05259 )
  • 体細胞クローン動物由来腎臓移植モデルによるマイナー組織適合性抗原の評価 (研究課題/領域番号: 26560212 )
  • 飼養環境下の牛群における複数のマイコトキシンによる共浸潤動態とその影響の解明 (研究課題/領域番号: 26450429 )
  • モンゴルにおける交雑種ヤクに発生する不妊性の実態解明と交雑種オスからの産子作出 (研究課題/領域番号: 25304039 )
  • 非コードRNA作用複合体・クロマトイド小体による半数体ゲノム遺伝子量補正機構解明 (研究課題/領域番号: 24570237 )
  • 飼養環境下の家畜におけるゼアラレノン浸潤動態の解明と対策法の確立 (研究課題/領域番号: 23580441 )
  • 人工染色体ベクターを活用した遺伝子改変動物の開発 (研究課題/領域番号: 22580320 )
  • 飼養環境下の牛群におけるゼアラレノン浸潤動態の解明-特に繁殖性との関連性の検証 (研究課題/領域番号: 20580355 )
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