徳島大学 / 教育研究者総覧 --- 柿本拓海

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徳島大学 ⟩ 病院 ⟩ 診療科 ⟩ 病理診断科 ⟩

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著書・論文

学術論文(審査論文):

1.	Takumi Kakimoto, Masato Hosokawa, Mayuko Shimizu, Hirohisa Ogawa, Yuko Miyakami, Satoshi Sumida and Koichi Tsuneyama : Accumulation of α-synuclein in hepatocytes in nonalcoholic steatohepatitis and its usefulness in pathological diagnosis., Pathology, Research and Practice, Vol.247, 154525, 2023. (要約) Nonalcoholic steatohepatitis (NASH) is characterized by fat deposition, inflammation, and hepatocellular damage. The diagnosis of NASH is confirmed pathologically, and hepatocyte ballooning is an important finding for definite diagnosis. Recently, α-synuclein deposition in multiple organs was reported in Parkinson's disease. Since it was reported that α-synuclein is taken up by hepatocytes via connexin 32, the expression of α-synuclein in the liver in NASH is of interest. The accumulation of α-synuclein in the liver in NASH was investigated. Immunostaining for p62, ubiquitin, and α-synuclein was performed, and the usefulness of immunostaining in pathological diagnosis was examined. Liver biopsy tissue specimens from 20 patients were evaluated. Several antibodies against α-synuclein, as well as antibodies against connexin 32, p62, and ubiquitin were used for immunohistochemical analyses. Staining results were evaluated by several pathologists with varying experience, and the diagnostic accuracy of ballooning was compared. Polyclonal α-synuclein antibody, not the monoclonal antibody, reacted with eosinophilic aggregates in ballooning cells. Expression of connexin 32 in degenerating cells was also demonstrated. Antibodies against p62 and ubiquitin also reacted with some of the ballooning cells. In the pathologists' evaluations, the highest interobserver agreement was obtained with hematoxylin and eosin (H&E)-stained slides, followed by slides immunostained for p62 and α-synuclein, and there were cases with different results between H&E staining and immunostaining CONCLUSION: These results indicate the incorporation of degenerated α-synuclein into ballooning cells, suggesting the involvement of α-synuclein in the pathogenesis of NASH. The combination of immunostaining including polyclonal α-synuclein may contribute to improving the diagnosis of NASH. (キーワード) α-synuclein / NASH / Ballooning (徳島大学機関リポジトリ) ● Metadata: 118741 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.prp.2023.154525 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37209576 ● Search Scopus @ Elsevier (PMID): 37209576 ● Search Scopus @ Elsevier (DOI): 10.1016/j.prp.2023.154525 (徳島大学機関リポジトリ: 118741, DOI: 10.1016/j.prp.2023.154525, PubMed: 37209576)
2.	Satoshi Sumida, Mayuko Shimizu, Yuko Miyakami, Takumi Kakimoto, Tomoko Kobayashi, Yasuyo Saijo, Minoru Matsumoto, Hirohisa Ogawa, Takeshi Oya, Yoshimi Bando, Hisanori Uehara, Shu Taira, Mitsuo Shimada and Koichi Tsuneyama : Histological and immunohistochemical analysis of epithelial cells in epidermoid cysts in intrapancreatic accessory spleen., The Journal of Medical Investigation : JMI, Vol.70, No.1.2, 251-259, 2023. (要約) Novel histological features of epithelial cells of ECIPAS were indicated. Although more cases need to be evaluated, we propose that the cause of ECIPAS may be different from that of pancreatic ductal origin. J. Med. Invest. 70 : 251-259, February, 2023. (キーワード) Humans / Epidermal Cyst / Spleen / Pancreatic Diseases / Epithelial Cells / Immunohistochemistry (徳島大学機関リポジトリ) ● Metadata: 118205 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.70.251 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37164730 ● Search Scopus @ Elsevier (PMID): 37164730 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.70.251 (徳島大学機関リポジトリ: 118205, DOI: 10.2152/jmi.70.251, PubMed: 37164730)
3.	Kohei Muto, Ryosuke Miyamoto, Yuka Terasawa, Yoshimitsu Shimatani, Keijiro Hara, Takumi Kakimoto, Tatsuya Fukumoto, Yusuke Osaki, Koji Fujita, Masafumi Harada, Hisanori Uehara, Yasushi Takagi and Yuishin Izumi : A novel COL4A1 variant associated with recurrent epistaxis and glioblastoma, Human Genome Variation, Vol.8, No.1, 18, 2021. (徳島大学機関リポジトリ) ● Metadata: 116530 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41439-021-00150-0 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85105944271 (徳島大学機関リポジトリ: 116530, DOI: 10.1038/s41439-021-00150-0, Elsevier: Scopus)

学術論文(紀要・その他):

1.	松本力三, 西尾進, 平田有紀奈, 森田沙瑛, 湯浅麻美, 山尾雅美, 柿本拓海, 齋藤裕, 楠瀬賢也, 山田博胤, 上原久典, 島田光生, 佐田政隆 : 術前の画像検査で肝細胞癌と診断された肝炎症性偽腫瘍の1例, 超音波検査技術, Vol.48, No.5, 499-508, 2023年. (要約) 症例は70代,男性.近医の定期的な血液検査で胆道系酵素の上昇を認めたため,腹部超音波検査を施行したところ,肝左葉内側区に腫瘤を認めた.当院消化器外科に紹介となり,肝内側区切除術が施行され,肝細胞癌と診断された.術後7ヵ月,当院で経過観察目的に施行した造影CT検査で肝左葉外側区に新たに腫瘤を認め,再度精査となった.血液検査では,γ-GTPの軽度上昇を認めるのみで,腫瘍マーカーの上昇は認めなかった.術前の精査目的に施行した腹部超音波検査では,肝左葉外側区(S2)に18×16mm大の腫瘤を認めた.境界明瞭,輪郭は後面が整,前面がやや不整,内部エコーは等輝度で不均質であった.辺縁に比較的厚い低エコー帯を伴っていた.心拍動の影響で血流シグナルの評価は困難であった.腹部造影CT検査では,肝左葉外側区(S2)に2cm大の腫瘤を認め,早期濃染および洗い出しが確認された.本症例は,術前の超音波検査で,肝細胞癌としては非典型的な像を呈していたが,他の画像所見および既往歴から肝細胞癌の再発が否定できず,肝部分切除術が施行され,肝炎症性偽腫瘍と診断された.本症例のように肝細胞癌のリスクが高い症例においても,新規に腫瘤を認めた際には,超音波検査でその特徴を注意深く観察し,典型像と異なる場合には肝炎症性偽腫瘍も鑑別疾患の一つとして考慮した方がよいと考える.(著者抄録) (キーワード) 肝炎(画像診断,外科的療法,病理学) 肝細胞癌肝切除 MRI 腫瘍再発鑑別診断術前評価超音波診断 X線CT 肉芽腫(画像診断,外科的療法,病理学) 肉芽腫-形質細胞(画像診断,外科的療法,病理学) Gadoxetic Acid(診断的利用) Azan染色肝肉芽腫(画像診断,外科的療法,病理学) 腹部CT ヒト高齢者(65∼79) 男 (出版サイトへのリンク) ● Publication site (DOI): 10.11272/jss.405 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.11272/jss.405 (DOI: 10.11272/jss.405)

国際会議:

1.	Koichi Tsuneyama, Takumi Kakimoto and Mayuko Shimizu : Connexin 32 expression and alpha-synuclein accumulation in ballooning cells of non-alcoholic steatohepatitis (NASH) and other inflammatory liver diseases, AASLD 2024, Nov. 2023.
2.	Takumi Kakimoto, Mayuko Shimizu, Koichi Tsuneyama, Minoru Matsumoto, Takeshi Oya, Hosokawa Masato and Koichi Tsuneyama : Accumulation of α-synuclein in hepatocytes in NASH liver and usefulness in pathological diagnosis, Digestive Disease Week 2022, May 2022.

国内講演発表:

1.	柿本拓海, 清水真祐子, 小川博久, 常山幸一 : 非アルコール性脂肪性肝炎の診断におけるα-synuclein免疫組織化学染色の有用性, 日本病理学会会誌, Vol.110, No.2, 102, 2021年10月. (キーワード) 免疫組織化学 Alpha-Synuclein *肝疾患-非アルコール性脂肪性(病理学) ヒト