Tokushima University / Educator and Researcher Directory --- Tsukimoto, Jun

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Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 薬学域 ⟩ Pharmaceutical Science ⟩ Integrative Medicinal Sciences ⟩ Department of Bioorganic Chemistry ⟩

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Research

Field of Study

Subject of Study

Book / Paper

Book:

1.	Jun Tsukimoto and Kouji Itou : ガラクトシアリドーシス, Oct. 2023.

Academic Paper (Judged Full Paper):

1.	Tanaka Hiroki, Daisuke Tsuji, Watanabe Ryosuke, Ohnishi Yukiya, Kitaguchi Shindai, Nakae Ryuto, Teramoto Hiromi, Jun Tsukimoto, Horii Yuto and Kouji Itou : Aberrant autophagy in lysosomal storage disorders marked by a lysosomal SNARE protein shortage due to suppression of endocytosis, Journal of Inherited Metabolic Disease, Vol.45, No.6, 1191-1202, 2022. (Summary) Lysosomal storage disorders (LSDs) are inherited metabolic diseases caused by genetic defects in lysosomal enzymes or related factors. LSDs are associated with excessive accumulation of natural substrates in lysosomes leading to central nervous system and peripheral tissue damage. Abnormal autophagy is also involved in pathogenesis, although the underlying mechanisms remain unclear. We demonstrated that impairment of lysosome-autophagosome fusion is due to suppressed endocytosis in LSDs. The fusion was reduced in several LSD cells and the brains of LSD model mice, suggesting that the completion of autophagy is suppressed by the accumulation of substrates. In this brain, the expression of the soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins, VAMP8 and Syntaxin7, was decreased on the lysosomal surface but not intracellular. This aberrant autophagy preceded the development of pathological phenotypes in LSD-model mice. Furthermore, the enzyme deficiency leading to the substrate accumulation could suppress endocytosis, and the inhibited endocytosis decreased SNARE proteins localized on lysosomes. These findings suggest that the shortage of SNARE proteins on lysosomes is one of the reasons for the impairment of lysosome-autophagosome fusion in LSD cells. Defects in lysosomal enzyme activity suppress endocytosis and decrease the supply of intracellular SNARE proteins recruited to lysosomes. This shortage of lysosomal SNARE proteins impairs lysosome-autophagosome fusion in lysosomal storage disorders. (Keyword) Animals / Mice / Autophagy / Endocytosis / Lysosomal Storage Diseases / Lysosomes / SNARE Proteins (Link to Publication Site) ● Publication site (DOI): 10.1002/jimd.12558 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36102069 ● Search Scopus @ Elsevier (PMID): 36102069 ● Search Scopus @ Elsevier (DOI): 10.1002/jimd.12558 (DOI: 10.1002/jimd.12558, PubMed: 36102069)

Review, Commentary:

1.	Jun Tsukimoto and Kouji Itou : 細胞内結晶化能を持つノイラミニダーゼ1と欠損症の治療法開発, Seikagaku, Vol.95, No.6, 784-791, Dec. 2023.
2.	Jun Tsukimoto and Kouji Itou : Human neuraminidase 1 and related diseases, Trends in Glycoscience and Glycotechnology, Vol.35, No.206, E53-E55, Jul. 2023.

Proceeding of International Conference:

1.	AKIHO Murai, Noriko Saito-Tarashima, OBA Mizuki, Kanako Kawanishi, Jun Tsukimoto and Noriaki Minakawa : Disruption of the cell membrane by G-quadruplex formation on antibody, Supra FIBER International Summit for Nucleic Acids (S-FISNA) 2024, Mar. 2024.
2.	Jun Tsukimoto, Miyoshi Mizuki, Fukuike Rin, Takeuchi Yoshie, Horii Yuto, Iniwa Toshiki, Nishioka So-ichiro and Kouji Itou : Inhibition of in cellulo crystallization of human neuraminidase 1 and application for gene therapy of lysosomal storage disease, Sialoglyco 2022, Sep. 2022.

Proceeding of Domestic Conference:

1.	Jun Tsukimoto, RIN Fukuike, Mizuki Miyoshi, Yuto Horii, Toshiki Iniwa, Nijiho Kamori, Yoshie Takeuchi, 西岡宗一郎, Noriko Saito-Tarashima, Noriaki Minakawa and Kouji Itou : NEU1欠損症モデルマウスの作製と新規遺伝子治療法開発, 日本薬学会第144年会, Mar. 2024.
2.	OBA Mizuki, AKIHO Murai, Noriko Saito-Tarashima, Jun Tsukimoto and Noriaki Minakawa : Antibody-Ologonucleotide Conjugate (AOC)を利用する光応答性抗体凝集法の開発, 日本薬学会第144年会, Mar. 2024.
3.	KURODA Chihiro, YUTA Kashiwabara, Jun Tsukimoto, Noriko Saito-Tarashima and Noriaki Minakawa : 4'-チオヌクレオチドの導入による化学修飾mRNAの開発, 日本薬学会第144年会, Mar. 2024.
4.	YUHEI Nogi, Noriko Saito-Tarashima, Takaaki Koma, Jun Tsukimoto, Masako Nomaguchi and Noriaki Minakawa : 抗SARS-CoV-2活性を指標とした4'-チオ修飾siRNAの最適化, 日本薬学会第144年会, Mar. 2024.
5.	Rina Ozaki, YUHEI Nogi, Noriko Saito-Tarashima, Takaaki Koma, Jun Tsukimoto, Masako Nomaguchi and Noriaki Minakawa : 4'-チオ核酸修飾siRNAの開発 (2), 第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, Oct. 2023.
6.	Naoshi Yamazaki, Airi Ohkawa, Shiori Yamamoto, Arisa Biwatani, Jun Tsukimoto, Kouji Itou and Kentaro Kogure : 塩基改変U1 snRNAを用いたカテプシンAスプライス異常の修復, 第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, Oct. 2023.
7.	Jun Tsukimoto, Mizuki Miyoshi, RIN Fukuike, Yuto Horii, Nijiho Kamori, Yoshie Takeuchi, Noriko Saito-Tarashima, Noriaki Minakawa and Kouji Itou : ノイラミニダーゼ1細胞内結晶化の抑制とリソソーム病遺伝子治療への応用, 日本核酸医薬学会第8回年会, Jul. 2023.
8.	Airi Ohkawa, Shiori Yamamoto, Arisa Biwatani, Jun Tsukimoto, Kouji Itou, Kentaro Kogure and Naoshi Yamazaki : 改変 U1 snRNA を用いたカテプシン A スプライス異常の修復, 第64回日本生化学会中国四国支部例会, May 2023.
9.	Jun Tsukimoto, Mizuki Miyoshi, RIN Fukuike, Yuto Horii, Toshiki Iniwa, Nijiho Kamori, Yoshie Takeuchi, 西岡宗一郎 and Kouji Itou : ノイラミニダーゼ1(NEU1)細胞内結晶化阻止とNEU1欠損症の治療, 第63回日本先天代謝異常学会学術集会, Nov. 2022.
10.	Jun Tsukimoto, Mizuki Miyoshi, RIN Fukuike, Yuto Horii, Toshiki Iniwa, Nijiho Kamori, Yoshie Takeuchi, 西岡宗一郎 and Kouji Itou : ノイラミニダーゼ1細胞内結晶化抑制とリソソーム病治療応用, 第95回日本生化学会大会, Nov. 2022.
11.	RIN Fukuike, Jun Tsukimoto, Yuto Horii, Yoshie Takeuchi, Nijiho Kamori, Mizuki Miyoshi and Kouji Itou : 改変型NEU1/CTSA遺伝子二重搭載AAVPHP.eBベクターによるミオクローヌス発症マウスの遺伝子治療, 第63回日本生化学会中国・四国支部例会, May 2022.
12.	TERU Mizuno, Jun Tsukimoto, Yuto Horii, Yoshie Takeuchi and Kouji Itou : TGカイコ繭由来ヒトCTSA及び人口糖鎖改変体のCTSA欠損症モデルマウス脳室内及び静脈内への補充効果解析, 第63回日本生化学会中国・四国支部例会, May 2022.
13.	Mizuki Miyoshi, Jun Tsukimoto, Yuto Horii, Yoshie Takeuchi, Nijiho Kamori, RIN Fukuike and Kouji Itou : 細胞内結晶化抑制型NEU1及びCTSA遺伝子同時搭載AAV5を用いたガラクトシアリドーシスモデルマウスに対する遺伝子治療法開発, 第63回日本生化学会中国・四国支部例会, May 2022.
14.	Jun Tsukimoto, Yoshie Takeuchi, Toshiki Iniwa, Yuto Horii, 西岡宗一郎 and Kouji Itou : ヒトノイラミニダーゼ1細胞内結晶化に対する付加N型糖鎖の影響, 第63回日本生化学会中国・四国支部例会, May 2022.
15.	Rina Takahashi, Momoka Ueta, Kanae Koide, Mao Kawai, Naoshi Yamazaki, Jun Tsukimoto, Kouji Itou and Yoshiharu Takiguchi : イントロンやエクソン内の配列と結合する改変U1 snRNAによるCTSAエクソン7スプライス異常の修復, 第58回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, Nov. 2019.
16.	四宮槙子, 小出華永, Rina Takahashi, Naoshi Yamazaki, Jun Tsukimoto, Kouji Itou and Yoshiharu Takiguchi : Exon specific U1 snRNAによる CTSAエクソン7スプライス異常の修復, 第57回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, Nov. 2018.