Tokushima University / Educator and Researcher Directory --- Nakagawa, Hideyuki

Field of Study

○	Bioactive Substance Physiology
○	Cell Biology

Subject of Study

○	Comparative physiology and biochemistry of marine bioactive substances (sea urchin, scorpionfish, mitogenic activity, cytotoxicity, bioactive substance, lectin, differentiation, apoptosis) (I have been studying for twenty years on sea urchin bioactive substances. More recently, I have also studied on fish venoms from scorpionfish. Our recent data revealed the isolation and some characterization of D-galactose-specific lectins (SUL-I and SUL-II) and a heparin-binding lectin from the toxoneustid sea urchins. Especially, SUL-I has mitogenic stimulation on T-cells, chemotactic properties for neutrophils, and regulates mast cell function. On the other hand, two venoms from scorpionfish have mitogenic and cytotoxic activity. A glycoprotein was isolated from the dorsal spines of redfin velvetfish. The glycoprotein with a molecular mass of 110 kDa has cytotoxic activity on murine leukemic cells.)
○	Comparative Physiology and Biochemistry of Marine Bioactive Substances

Book / Paper

Book:

1.	edited T. Tu Anthony, 後藤京子, Hideyuki Nakagawa and 宮澤啓輔 : Science in Action Series 51 :Social poison, toxin and venom, Tokyo Kagaku Dozin C., Ltd., Tokyo, Jun. 2012. (Keyword) 日常生活に潜む毒物 / 海に潜む毒物 / 咬毒 / 食中毒 / 刺毒
2.	石川統, 他6名編集, Hideyuki Nakagawa and 他800名 : ウニ毒，魚毒, Tokyo Kagaku Dozin C., Ltd., Dec. 2010. (Keyword) 生物 / 用語辞典
3.	石川統, 黒岩常祥, 塩見正衛, 松本忠夫, 守隆夫, 八杉貞雄, 山本正幸, Hideyuki Nakagawa and 他800名 : 生物学辞典, Tokyo Kagaku Dozin C., Ltd., Tokyo, Dec. 2010. (Keyword) 生物学辞典
4.	Hideyuki Nakagawa : 薬毒物分析辞典(編集:高取健彦，黒岩幸雄，Anthony T. Tu，山本郁男), 株式会社廣川書店, Tokyo, Sep. 2009. (Keyword) sea urchin lectin
5.	Anthony T. Tu, Shinichi Miyoshi, Hideaki Miyata, Shigeru Shintani and Hideyuki Nakagawa : Dictionary of Toxic Substances, Kagaku-Dojin Publishing Company,INC, Kyoto, Jul. 2005. (Summary) 毒や中毒に関連する重要な用語を分かりやすく解説し，専門家だけでなく一般の人びとに役立つようなハンディな辞典．主な内容は，ドーピング/キノコや間違えて食べてしまう植物の毒/海産毒(海洋毒液，海洋毒素)/ヘビ毒/昆虫毒(ノミ，ダニ，ハチ等)/細菌毒/化学·生物兵器/日常品による毒/薬の中毒/重金属中毒/農薬/覚せい剤，麻薬，幻覚剤/皮膚のかぶれ，アレルギー症，花粉症/中毒の救急法など． (Keyword) Marine venom / Marine toxin / Fish venom / Sea urchin venom / Sponge toxins / venomous scorpionfish / Venom of echinodermata / Venom of chidarians / Sea snake venom / Fish skin toxins
6.	Hideyuki Nakagawa, Tamiko Hashimoto, Hiromi Hayashi, Mitsuko Shinohara, Kiyoshi Ohura, Eiichi Tachikawa and Takeshi Kashimoto : Isolation of a novel lectin from the globiferous pedicellariae of the sea urchin Toxoneustes pileolus, Plenum Press, New York, Apr. 1996. (Summary) 分子量3.2 万のウニレクチン–I (SUL-I) はラッパウニの大型叉棘からゲル濾過，イオン交換クロマトグラフィおよび逆相 HPLC により精製された．S型レクチンと推定されるSUL-I は D - ガラクトース結合性レクチンで，アミノ酸294残基よりなり，モルモット好中球に対して遊走活性を示した． (Keyword) Sea urchin / Toxoneustes pileolus / Lectin / D-galacotose / Chemotaxis

Academic Paper (Judged Full Paper):

1.	Tomomitsu Hatakeyama, Ayaka Ichise, Hideaki Unno, Shuichiro Goda, Tatsuya Oda, Hiroaki Tateno, Jun Hirabayashi, Hitomi Sakai and Hideyuki Nakagawa : Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus, Protein Science, Vol.276, No.8, 1574-1583, 2017. (Summary) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure. (Keyword) Amino Acid Sequence / Animal Structures / Animals / Binding Sites / Carbohydrate Sequence / Cercopithecus aethiops / Cloning, Molecular / X-ray crystallography / Escherichia coli / gene expression / Hydrogen Bonding / Lectins / Lymphocytes / Marine Toxins / Mice / Microarray Analysis / Mitogens / Models, Molecular / Polysaccharides / Protein Binding / Protein Interaction Domains and Motifs / Protein Multimerization / Protein Structure, Secondary / Recombinant Proteins / Rhamnose / Sea Urchins / Vero Cells (Link to Publication Site) ● Publication site (DOI): 10.1002/pro.3185 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28470711 ● Summary page in Scopus @ Elsevier: 2-s2.0-85018917301 (DOI: 10.1002/pro.3185, PubMed: 28470711, Elsevier: Scopus)
2.	Tomomitsu Hatakeyama, Ayaka Ichise, Tomokazu Yonekura, Hideaki Unno, Shuichiro Goda and Hideyuki Nakagawa : cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom, Toxicon, Vol.94, No.1, 8-15, 2015. (Summary) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contain several biologically active proteins. Among these, a galactose-binding lectin SUL-I isolated from the venom in the large globiferous pedicellariae shows several activities such as mitogenic, chemotactic, and cytotoxic activities through binding to the carbohydrate chains on the cells. We cloned cDNA encoding SUL-I by reverse transcription-PCR using the degenerate primers designed on the basis of the N-terminal amino acid sequence of the protein and expressed the recombinant SUL-I (rSUL-I) in Escherichia coli cells. The SUL-I gene contains an open reading frame of 927 nucleotides corresponding to 308 amino acid residues, including 24 residues of a putative signal sequence. The mature protein with 284 residues is composed of three homologous regions, each showing similarity with the carbohydrate-recognition domains of the rhamnose-binding lectins, which have been mostly found in fish eggs. While rSUL-I exhibited binding activity for several galactose-related sugars, the highest affinity was found for l-rhamnose among carbohydrates tested, confirming that SUL-I is a rhamnose-binding lectin. rSUL-I also showed hemagglutinating activity toward rabbit erythrocytes, indicating the existence of more than one carbohydrate-binding site to cross-link the carbohydrate chains on the cell surface, which may be closely related to its biological activities. (Keyword) sea urchin venom / Toxopneustes pileolus / pedicellaria / rhmnose-binding lectin / cDNA cloning (Link to Publication Site) ● Publication site (DOI): 10.1016/j.toxicon.2014.11.236 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25475394 ● Summary page in Scopus @ Elsevier: 2-s2.0-84918582853 (DOI: 10.1016/j.toxicon.2014.11.236, PubMed: 25475394, Elsevier: Scopus)
3.	Hideyuki Nakagawa, Kuniko Nagasaka, Hitomi Sakai, Kozue Edo, Mitsuko Shinohara and kiyoshi Ohura : Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus, International Aquatic Research, Vol.7, No.2, 143-150, 2015. (Link to Publication Site) ● Publication site (DOI): 10.1007/s40071-015-0101-2 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/s40071-015-0101-2 (DOI: 10.1007/s40071-015-0101-2)
4.	Hitomi Sakai, Kozue Edo, Hideyuki Nakagawa, Mitsuko Shinohara, Rie Nishiitsutsuji and Kiyoshi Ohura : Isolation and partial characterization of a L-rhamnose-binding lectin from the globiferous pedicellariae of the toxopneustid sea urchin, Toxopneustes pileolus, International Aquatic Research, Vol.5, No.12, 1-10, 2013. (Keyword) Sea urchin / Toxopneustes pileolus / L-rhamnose-binding lectin / Mitogenic activity / Helper T cell (Link to Publication Site) ● Publication site (DOI): 10.1186/2008-6970-5-12 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1186/2008-6970-5-12 (DOI: 10.1186/2008-6970-5-12)
5.	Kozue Edo, Hitomi Sakai, Hideyuki Nakagawa, Tamiko Hashimoto, Mitsuko Shinohara and Kiyoshi Ohura : Immunomodulatory activity of a pedicellarial venom lectin from the toxopneustid sea urchin, Toxopneustes pileolus, Toxin Reviews, Vol.31, No.3-4, 54-60, 2012. (Keyword) toxopneustid sea urchin / venom / D-galactose-binding lectin / inflammatory cytokine / Th-related cytokine (Link to Publication Site) ● Publication site (DOI): 10.3109/15569543.2012.730090 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84869470363 (DOI: 10.3109/15569543.2012.730090, Elsevier: Scopus)
6.	Mitsuko Shinohara, Kuniko Nagasaka, Hideyuki Nakagawa, Kozue Edo, Hitomi Sakai, Koji Kato, Futoshi Iwaki, Kiyoshi Ohura and Haruhiko Sakuraba : A novel chemoattractant lectin, Karatoxin, from the dorsal spines of the small scorpionfish Hypodytes rubripinnis, Journal of Pharmacological Sciences, Vol.113, No.4, 414-417, 2010. (Summary) A chemoattractant lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, was isolated using a combination of affinity chromatography techniques. The glycoprotein, with a molecular mass of 110 kDa, is named Karatoxin. Karatoxin caused agglutination of rabbit erythrocytes. This agglutination was effectively inhibited by D-mannose. In addition, Karatoxin exhibited not only mitogenic activity in the presence of murine splenocytes, but also chemotactic activity in the presence of guinea-pig neutrophils and macrophages. Thus, Karatoxin appears to be a novel chemoattractant lectin. These results suggest that the redfin velvetfish Hypodytes rubripinnis may be a novel source of biologically active substances. (Keyword) Karatoxin / Scorpionfish / Hypodytes rubripinnis / Cemoattractant lectin (Link to Publication Site) ● Publication site (DOI): 10.1254/jphs.10058SC (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20647688 ● Summary page in Scopus @ Elsevier: 2-s2.0-77956213737 (DOI: 10.1254/jphs.10058SC, PubMed: 20647688, Elsevier: Scopus)
7.	Kuniko Nagasaka, Hideyuki Nakagawa, Fumihiko Satoh, Takefumi Hosotani, Kumio Yokoigawa, Hitomi Sakai, Haruhiko Sakuraba, Toshihisa Ohshima, Mitsuko Shinohara and Kiyoshi Ohura : A novel cytotoxic protein, Karatoxin, from the dorsal spines of the the redfin velvetfihi, Hypodytes rubripinnis, Toxin Reviews, Vol.28, No.4, 260-265, 2009. (Keyword) Hypodytes rubripinnis / Karatoxin (Link to Publication Site) ● Publication site (DOI): 10.3109/15569540903348940 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-72049118862 (DOI: 10.3109/15569540903348940, Elsevier: Scopus)
8.	Masao Takei and Hideyuki Nakagawa : A sea urchin lectin, SUL-1, from the Toxopneustid sea urchin induces DC maturation from human monocytes and drives Th1 polarization in vitro, Toxicology and Applied Pharmacology, Vol.213, No.1, 27-36, 2006. (Summary) The sea urchin Toxopneustes pileolus belonging to the family Toxopneustidae, they have well-developed globiferous pedicellariae with pharmacologically active substances. We have purified a novel sea urchin lectin-1 (SUL-1) from the large globiferous pedicellariae of T. pileolus. Dendritic cells (DC) are professional APC and play a pivotal role in controlling immune responses. This study investigated whether SUL-1 can drive DC maturation from human immature monocyte-derived DC in vitro. Human monocytes were cultured with GM-CSF and IL-4 for 6 days followed by another 1 day in the presence of SUL-1 or LPS. DC harvested on day 7 were examined using functional assays. The expression levels of CD1a, CD80, CD83, CD86 and HLA-DR as expressed by mean fluorescence intensity (MFI) on DC differentiated from immature DC after culture with 1.0 microg/ml of SUL-1 for 1 day were enhanced and decreased endocytic activity. SUL-1-treated DC also displayed enhanced T cell stimulatory capacity in an MLR, as measured by T cell proliferation. Cell surface expression of CD80, CD83 and CD86 on SUL-1-treated DC was inhibited by anti-DC-SIGN mAb, while anti-DC-SIGN mAb had no influence on allogeneic T cell proliferation by SUL-1-treated DC. DC differentiated with SUL-1 induced the differentiation of naïve T cell towards a helper T cell type 1 (Th1) response at DC/T (1:5) cells ratio depending on IL-12 secretion. In CTL assay, the production of IFN-gamma and 51Cr release on SUL-1-treated DC were more augmented than of immature DC or LPS-treated DC. SUL-1-treated DC expressed CCR7 and had a high migration to MIP-3beta. Intracellular Ca2+ mobilization in SUL-1-treated DC was also induced by MIP-3beta. These results suggest that SUL-1 bindings to DC-SIGN on surface of immature DC may lead to differentiate DC from immature DC. Moreover, it suggests that SUL-1 may be used on DC-based vaccines for cancer immunotherapy. (Keyword) Animals / Cell Adhesion Molecules / cell differentiation / Cell Polarity / Cells, Cultured / Cytotoxicity, Immunologic / Dendritic Cells / Endocytosis / Humans / Interleukin-12 / Interleukin-4 / Lectins / Lectins, C-Type / Lipopolysaccharides / Lymphocyte Culture Test, Mixed / Monocytes / Receptors, Cell Surface / Sea Urchins / Th1 Cells (Link to Publication Site) ● Publication site (DOI): 10.1016/j.taap.2005.08.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16197973 ● Summary page in Scopus @ Elsevier: 2-s2.0-33646160189 (DOI: 10.1016/j.taap.2005.08.004, PubMed: 16197973, Elsevier: Scopus)
9.	Hideyuki Nakagawa, Takahiko Tanigawa, Katsuyuki Tomita, Yasuhiro Tomihara, Yasutetsu Araki and Eiichi Tachikawa : Recent Studies on the Pathological Effects of Purified Sea Urchin Toxins, Toxin Reviews, Vol.22, No.4, 631-647, 2003. (Summary) 日本近海には約200種類のウニが生息し，その内有用種は15種類である．一方，棘に毒を持つ有毒種は数種が知られレいる．私達は類熱帯性および亜熱帯性の4種類のウニの体表にあるトゲから，生理活性物質として糖類に特異的に結合するレクチンを精製·分離して，それらの生物活性を検討した．ラッパウニ科のウニからはD-ガラクトース結合性レクチンが見い出され，細胞増殖活性を持つことがわかった．体表の長い棘に毒を持つガンガゼやリュウキュウフクロウニからはレクチンの存在は推定されたが，精製にはいたっていない．これらウニからのレクチンは，医薬品素材として可能性が強く示唆された． (Keyword) Lectin / Sea urchins / Sea urchin venom / Toxins / Biological activity (Link to Publication Site) ● Publication site (DOI): 10.1081/TXR-120026918 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0348014605 (DOI: 10.1081/TXR-120026918, Elsevier: Scopus)
10.	Hideyuki Nakagawa, Fumihiko Satoh, Hitomi Sakai, Hiromi Hayashi and Yasuhiro Ozeki : Biological activities of multiple lectins from the toxopneustid sea urchins, Recent Advances in Marine Science and Technology 2002, 145-152, 2003. (Summary) ラッパウニ科のラッパウニおよびシラヒゲウニの毒棘のタンパク画分をゲル濾過，アフィニティークロマトグラフィーおよびHPLCにより精製し，レクチンを単離し，それらの生物活性を比較検討した．ラッパウニから精製された32 kDa のSUL-I は多様な生物活性を示したが，SUL-II はほとんど細胞増殖活性などの生理活性を示さなかった．一方，シラヒゲウニから精製された23 kDa のヘパリン結合性のレクチン，TGL-I も細胞増殖活性は示さなかった．SUL-I とSUL-II は部分アミノ酸配列において殆ど相同性は認められなかった． (Keyword) sea urchin / lectin / mitogenic activity / amino acid
11.	Hiromi Hayashi, Hitomi Sakai, Wakako Minakuchi-Fijiwara, Miki Takayama, Michiko Nakamura-Murata, Yoshiko Kamo, Kanako Funakoshi, Keisuke Fukumoto, Kaori Kanemaru, Hideyuki Nakagawa, Yasuo Oyama, Nobuyuki Shinohara and Yoshihiro Ito : Cytokinesis arrest and nuclear fission in low density populations of Trichomonad Protozoan, Zoological Science, Vol.19, No.10, 1089-1094, 2002. (Summary) Cell growth of anaerobic protozoan Tritrichomonas foetus was analyzed. This protozoan usually proliferates in extremely high density, but protozoan parasites were dispersed uniformly in F-bouillon medium and cell division stopped temporarily. However, nuclear fission continued and giant polynucleated cells formed. Later, cell division resumed and cells returned to normal form. In conditioned medium, cytokinesis of the dispersed parasites did not stop. Results indicated that T. foetus cells secreted an extracellular factor that influenced cytokinesis. (Keyword) Animals / Cell Division / Cell Nucleus / Culture Media / Oxygen / Population Density / Time Factors / Trichomonas (Link to Publication Site) ● Publication site (DOI): 10.2108/zsj.19.1089 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12426470 ● CiNii @ National Institute of Informatics (CRID): 1522825128326665984 ● Search Scopus @ Elsevier (PMID): 12426470 ● Search Scopus @ Elsevier (DOI): 10.2108/zsj.19.1089 (DOI: 10.2108/zsj.19.1089, PubMed: 12426470, CiNii: 1522825128326665984)
12.	Fumihiko Satoh, Hideyuki Nakagawa, Haruo Yamada, Kuniko Nagasaka, Toshinori Nagasaka, Yasutetsu Araki, Yasuhiro Tomihara, Masatoshi Nozaki, Haruhiko Sakuraba, Toshihisa Ohshima, Tomomitsu Hatakeyama and Haruhiko Aoyagi : Fishing for bioactive substances from scorpionfish and some sea urchins, Journal of Natural Toxins, Vol.11, No.4, 297-304, 2002. (Summary) Venom proteins from the dorsal spine of two scorpionfish, Hypodytes rubripinnis and Synanceia verrucosa were assayed for mitogenicity and cytotoxicity. The two venoms had both mitogenic and cytotoxic activity on murine splenocytes and murine P388 leukemic cells. In H. rubripinnis, the second gel chromatographic fraction showed cytotoxic activity on P388 leukemic cells. On native PAGE, the glycoprotein isolated by concavalin A sepharose chromatography appeared to have a molecular mass of 110 kDa. In addition, two D-galactose-binding lectins (SUL-I and SUL-II) and a heparin-binding lectin (TGL-I) were purified from the globiferous pedicellariae of the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla, respectively. SUL-I (Nakagawa et al., 1999a) had mitogenic activity and cytotoxic activity but SUL-II and TGL-I did not. SUL-I did not show sequence homology to SUL-II. A hemolytic lectin with a molecular mass of 29 kDa was isolated from the coelomic fluid of T. gratilla. The hemolytic activity of the lectin was dependent on Ca2+ concentration and inhibited by lactose. The present results suggest that some species of scorpionfish and sea urchins may be novel sources for biologically active substances such as anti-tumor compounds or new lectins. (Keyword) Sea urchin venom / Fish venom / SUL-I / Toxopneustes pileolus / Hypodytes rubripinnis (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12503872 ● Summary page in Scopus @ Elsevier: 2-s2.0-0036915041 (PubMed: 12503872, Elsevier: Scopus)
13.	Zhang Yu-an, Junichi Abe, Ambreena Siddiq, Hideyuki Nakagawa, Susumu Honda, Tetsuyuki Wada and Seiji Ichida : UT841 purified from sea urchin (Toxopneustes pileolus) venom inhibits time-dependent 45Ca²⁺ uptake in crude synaptosome fraction from chick brain, Toxicon, Vol.39, No.8, 1223-1229, 2001. (Summary) To clarify the mechanism by which the toxic abstract from Toxopneustes pileolus inhibits time-dependent (Time-dep.) Ca(2+) uptake in crude synaptosome fraction, the effective component from pedicellarial venom of the sea urchin was purified. The crude extracts were purified by a series of steps including ion exchange (DEAE-sephadex-A25 gel), gel filtration (with Superdex-2000 and Superdex-peptide columns) and reversed-phase chromatography (Sephasil-C18 column). The effective component that inhibited Time-dep. 45Ca(2+) uptake was purified and named UT841. Its IC(50) was determined to be lower than 35ng/ml. UT841 is an acidic protein with an apparent molecular weight of about 18,000. The N-terminal sequence (40 amino acids) was almost identical to that of Contractin A (a protein purified from the same kind of venom which induces smooth muscle contraction). Even though it is unclear whether or not UT841 is Contractin A, Ca(2+) mobilization in nerve cells was shown to be influenced by UT841. This investigation also revealed that a donor of nitric oxide, arachidonic acid and an inhibitor of phospholipase C selectively inhibit Time-dep. (45)Ca(2+) uptake. These results suggest that UT841 purified from sea urchin venom may affect Time-dep. (45)Ca(2+) uptake through the metabolism of some lipids and nitric oxide. (Keyword) Sea urchin venom / Lectin / Toxopneustes pileolus / Synaptosome / Ca uptake (Link to Publication Site) ● Publication site (DOI): 10.1016/S0041-0101(00)00267-1 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11306134 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035068012 (DOI: 10.1016/S0041-0101(00)00267-1, PubMed: 11306134, Elsevier: Scopus)
14.	Tamiko Suzuki-Nishimura, Hideyuki Nakagawa and Masaatsu Uchida : D-galactose-specific sea urchin lectin sugar-specifically inhibited histamine release induced by Datsura stramonium agglutinin, --- Differences between sugar-specific effects of sea urchin lectin and those of D-galactose-or L0fucose-specific plant lectins ---, The Japanese Journal of Pharmacology, Vol.85, No.4, 443-452, 2001. (Summary) A new sea urchin lectin from Toxopneustes pileolus, is D(+)galactose (Gal)-, D(+)fucose (Fuc)-specific. Incubation of rat peritoneal mast cells with the lectin in the presence of 0.3 mM CaCl2 for 10 min significantly and dose-dependently inhibited the histamine release induced by N-acetyl glucosamine (GlcNAc)-specific Datura stramonium agglutinin (DSA), an activator of the Gi-protein-dependent pathway in mast cells. This inhibition by the sea urchin lectin was sugar-specifically reversed in the presence of D(+)Gal or D(+)Fuc but not L(-)Fuc. The sea urchin lectin had no effect on the histamine release induced by compound 48/80, slightly inhibited the histamine release induced by substance P and mastoparan, and slightly enhanced the histamine release induced by melittin, but these effects were not dose-dependent. Compound 48/80, substance P, mastoparan and melittin are mast cell activators without sugar residues. It is suggested that the lectin binds to D(+)Gal residues of DSA to interfere with mast cell activation induced by DSA, a glycoprotein with arabinose and Gal residues. The effects of plant lectins with affinity to D(+)Gal, N-acetyl galactosamine and/or sialic acid and L(-)Fuc on the histamine release induced by DSA, compound 48/80 and substance P were also examined. (Keyword) Mast cell / Histamine release / Datsura stramonium agglutinin / Sea urchin lectin (Link to Publication Site) ● Publication site (DOI): 10.1254/jjp.85.443 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11388649 ● Summary page in Scopus @ Elsevier: 2-s2.0-0035012370 (DOI: 10.1254/jjp.85.443, PubMed: 11388649, Elsevier: Scopus)
15.	Zhang Yu-an, Tetsuyuki Wada, Yasunori Iwasaki, Hideyuki Nakagawa and Seiji Ichida : The inhibitory effect of the toxic fraction from sea urchin (Toxopneustes pileolus) venom on 45Ca²⁺ uptake in crude syaptosome fraction from chick brain, Biological & Pharmaceutical Bulletin, Vol.22, No.12, 1279-1283, 1999. (Summary) ラッパウニの大型叉棘標品のゲル濾過を行い，タンパク画分(P-I, P-II およびP-III画分)を回収した．これらの画分を用いて，ニワトリシナプトゾウムへの45Ca2+取り込みを検討した．その結果，P-II 画分はもっとも低濃度で経時的なNa+/Ca2+系への阻害を強く示唆した． (Keyword) Sea urchin / Toxopneustes pileolus / 45Ca2+uptake / Synaptosome
16.	Zhang Yu-an, Tetsuyuki Wada, Hideyuki Nakagawa and Seiji Ichida : A toxic extract from sea urchin (T. pileolus) inhibits 45Ca²⁺ uptake in P2 fraction from chick brain under physiologocal ionic conditions, Toxicon, Vol.37, No.11, 1639-1643, 1999. (Summary) ラッパウニの大型叉棘抽出標品は，ニワトリP2画分において時間依存的に45Ca2+の取り込みを阻害した．IC50値は10ng/ml であった．抽出標品による阻害はNa+/Ca2+系への影響と推定された． (Keyword) Sea urchin / Toxopneustes pileolus / Sea urchin venom / 45Ca2+ / Chick brain (Link to Publication Site) ● Publication site (DOI): 10.1016/S0041-0101(99)00094-X (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0033231655 (DOI: 10.1016/S0041-0101(99)00094-X, Elsevier: Scopus)
17.	Hideyuki Nakagawa, Chieko Yamaguchi, Fukiyo Tomiyoshi and Hiromi Hayashi : A novel mitogenic lectin from the globiferous pedicellariae of sea urchin, Toxopneustes pileolus, Jour. Chem. Soc. Pak., Vol.21, No.3, 305-310, 1999. (Summary) ラッパウニの大型叉棘から，D-ガラクトース結合性レクチンであるSUL-Iを精製した．マウス脾細胞に対してマイトジェン活性を示した．脾細胞からTリンパ球を分離して，T細胞の細胞増埴活性を検討したところ，SUL-Iは低濃度よりマイトジェン活性を示した．SUL-Iは新規な海洋生物レクチンであると考えられた． (Keyword) sea urchin / Toxopneustes pileolus / mitogenic stimulation / T cell / D-galactose-specific
18.	Hideyuki Nakagawa, Chieko Yamaguchi, Hitomi Sakai, Kaori Kanemaru, Hiromi Hayashi, Yasutetsu Araki, Yasuhiro Tomihara, Mitsuko Shinohara, Kiyoshi Ohura and Hisato Kitagawa : Biochemical and physiological properties of pedicellarial lectins from the toxopneustid sea urchins, Journal of Natural Toxins, Vol.8, No.3, 297-308, 1999. (Summary) Pedicellarial lectins (SUL-I, SUL-II, and TGL-I) were purified from the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla using gel filtration chromatography, affinity chromatography, and reverse-phase HPLC. SUL-I (Nakagawa et al., 1996) and SUL-II from the large globiferous pedicellariae of T. pileolus are D-galactose-binding lectins with molecular masses of 32 kDa and 23 kDa, respectively; while TGL-I from the globiferous pedicellariae of T. gratilla is a Ca(2+)-independent heparin-binding lectin with a molecular mass of 23 kDa. SUL-I induced mitogenic stimulation on murine splenocytes but TGL-I did not. At higher dose ranges SUL-I exhibited inhibitory effects on the cells. The dual response to SUL-I was effectively inhibited by D-galactose. SUL-I enhanced norepinephrine-induced contraction of isolated rat mesenteric artery with endothelium. When endothelium was removed from the artery, acetylcholine did not relax the norepinephrine-induced contraction. In the same artery the enhancing effect of the contraction by SUL-I was abolished, suggesting that SUL-I acts on the endothelium of mesenteric artery, and may release prostanoids. The present results suggest an extracellular function for SUL-I that may have wide-ranging effects in physiological processes. The primary role of pedicellarial lectins from T. pileolus and T. gratilla might be defense against a foreign body. (Keyword) Toxopneustes pileolus / Toxopneustes gratilla / D-galactose-specific lectin / Heparin-specific lectin / Contraction (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10591033 ● Search Scopus @ Elsevier (PMID): 10591033 (PubMed: 10591033)
19.	Hideyuki Nakagawa, Chieko Yamaguchi and Hiromi Hayashi : Biologically active subatance from sea urchins, Journal of Natural Toxins, Vol.6, No.2, 193-202, 1997. (Summary) ラッパウニ，シラヒゲウニ，ガンガゼおよびリュウキュウフクロウニの4種類のウニの棘蛋白室画分からレクチンを探索した．ウサギ赤血球の凝集反応を指標にレクチン画分を精製した．その結果，ラッパウニの叉棘蛋白画分より，Dーガラクトース結合性レクチンを精製した．分子量3.2 万のレクチンをSUL-I と命名した．SUL-I はマウス脾臓細胞および腸管膜リンパ球の細胞増殖活性を示した． (Keyword) Sea urchins / Lectin / Agglutination / Mitogenic activity / Toxopneustes pileolus
20.	Hideyuki Nakagawa, Yasuhiro Tomihara, Yasutetsu Araki and Hiromi Hayashi : Preliminary studies on venom proteins in the pedicellariae of the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla, Journal of Natural Toxins, Vol.3, No.1, 25-34, 1994. (Summary) ラッパウニ科のラッパウニとシラヒゲウニの叉棘蛋白質成分の比較を行った．両ウニの叉棘抽出標品をゲル濾過およびアフィニティクロマトグラフィーで分画した糖画分は生物活性には差異はなかったが，電気泳動的に質的な差異が認められた． (Keyword) Toxopneustid sea urchins / Venom protein / Toxoneustes pileolus / Tripneustes gratilla / Affinity chromatography
21.	Masao Takei, Hideyuki Nakagawa and Koichi Endo : Mast cell activation by pedicellarial toxin of sea uuchin, Toxopneustes pileolus, FEBS Letters, Vol.328, No.1,2, 59-62, 1993. (Summary) ラッパウニの叉棘タンパク質画分はラット腹腔肥満細胞からヒスタミン遊離を起した．同蛋白質画分はヒスタミン遊離を示す濃度で IP3 産生を増大させ，肥満細胞膜標品の PLC 活性化を起した．ホスホリパーゼC阻害剤で抑制された．Cholera toxin の遊離阻害の結果から，蛋白質画分は肥満細胞膜の G 蛋白質を活性化し，次いで PLC を活性化することが示唆された． (Keyword) Pedicellarial toxin / Sea urchin / Cholera toxin / PLC inhibitor / Mast cell activation
22.	Hideyuki Nakagawa, Nobuyuki Yanagihara, Fotoshi Izumi, Akihiko Wada and Akira Kimura : Inhibition of nicotinic acetylcholine receptor-madiated secretion and synthesis of catecholamines by sea urchin toxin in cultured bovine adrenal medullary cells, Biochemical Pharmacology, Vol.44, No.9, 1779-1785, 1992. (Summary) 培養ウシ副腎髄質細胞において，ラッパウニの叉棘由来のゲル濾過蛋白質画分はニコチン性アセチルコリン受容体刺激によるカテコールアミン遊離(CA) 分泌を抑制し，Ca および Na イオンの流入も抑制した．また同画分は受容体刺激における CA 生合成およびチロシン水酸化酵素活性を抑制した．これらの抑制作用はアセチルコリン受容体を介するナトリウムの流入を阻害することによるものであった． (Keyword) Sea urchin toxin / Catecholamines / Acetylcholine receptor / Adrenal medullary cells / Toxopneustes pileolus
23.	Hideyuki Nakagawa, Hisato Kitagawa, Noriaki Kondo and Jun Kondo : Effect of Contractin A, a glycoprotein from the pedicellarial venom of the sea uechin Toxoneustes pileolus, on isolated vasucular smooth muscles, Journal of Natural Toxins, Vol.1, No.2, 31-37, 1992. (Summary) マンノース糖鎖を含有する18 kDa の Contractin A はラット尾動脈の電気刺激収縮を濃度依存的に抑制したが，尾動脈のノルアドレナリン(NE) 収縮に対しては抑制を示さなかった．Contractin A の電気刺激収縮に対する抑制作用はNE 取り込み阻害薬により消失した．これらの結果はContactin A はラット尾動脈において，交感神経終末からのNE 遊離を抑制することを示唆した． (Keyword) Contractin A / Rat tail artery / A purified glycoprotein / Toxopneustes pileolus / Electrically-induced contraction
24.	Masao Takei, Makoto Ueno, Koichi Endo and Hideyuki Nakagawa : Effect of NCDC, a protease inhibitor, on histamine release from rat peritoneal mast cells induced by anti-IgE, Biochemical and Biophysical Research Communications, Vol.181, No.3, 1313-1322, 1991. (Summary) NCDC dose-dependently inhibited histamine release from rat peritoneal mast cells induced by anti-IgE. Moreover, NCDC inhibited Ca(2+)-mobilization from intracellular Ca(2+)-stores as well as histamine release in mast cells activated by anti IgE, the effect on both of these phenomena being closely correlated. Anti-IgE induced a rapid increase in IP3 production from phosphoinositides in mast cells, with its production in 15 sec, followed to baseline levels within 1 min. Anti-IgE stimulated PLC activity on mast cells membrane preparation. NCDC dose-dependently inhibited the generation of IP3. These results suggest that the inhibitory effect of NCDC on the release of histamine induced by anti-IgE is due to, in part at least, the inhibition of PI-specific PLC and that the inhibitory effects of NCDC are involved in intracellular calcium store. (Keyword) Mast cell / Histamine release / Protease inhibitor / NCDC / Anti-IgE / IP3 (Link to Publication Site) ● Publication site (DOI): 10.1016/0006-291X(91)92082-U (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 1722401 ● Summary page in Scopus @ Elsevier: 2-s2.0-0026322057 (DOI: 10.1016/0006-291X(91)92082-U, PubMed: 1722401, Elsevier: Scopus)
25.	Masao Takei, Hideyuki Nakagawa, Akira Kimura and Koichi Endo : A toxic substance from the sea urchin Toxopneustes pileolus induces histamine release from rat peritoneal mast cells, Agents and Actions, Vol.32, No.3/4, 224-228, 1991. (Summary) A toxic substance (P-II fraction), fractionated from the pedicellariae of the sea urchin Toxopneustes pileolus, dose-dependently caused the histamine release from rat peritoneal mast cells. The histamine release induced by P-II fraction increased with time, while compound 48/80 caused a more rapid histamine release. The dose-response curve for P-II fraction was studied with concentration 0.03-2.0 mg/ml. This reaction was dependent on Ca2+ and temperature. When glucose (5.5 mM) was omitted during the incubation step, the histamine release induced by P-II fraction was significantly reduced as compared to that of compound 48/80. Pyruvate reversed this reduction. On the other hand, the histamine release induced by P-II fraction was effectively potentiated by the addition of glucose (11.0 mM), but not that by compound 48/80. These results suggest that P-II fraction-induced histamine release differs from that of compound 48/80 disregards to the effects of glucose, because this histamine release appears to be more sensitive to the glycolytic pathway than compound 48/80-induced histamine release. (Keyword) Rat peritoneal mast cells / Histamin release / Sea urchin / Toxopneustes pileolus / Toxic substance / glucose (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 1713736 ● Summary page in Scopus @ Elsevier: 2-s2.0-0026028221 (PubMed: 1713736, Elsevier: Scopus)
26.	Hideyuki Nakagawa, Tu T. Anthony and Akira Kimura : Purification and characterization of Contractin A from the pedicellarial venom of sea urchin, Toxopneustes pileolus, Archives of Biochemistry and Biophysics, Vol.284, No.2, 279-284, 1991. (Summary) ラッパウニの中型叉棘から，マンノースを含有する糖タンパク質を精製した．この糖タンパク質は分子量1万8千で，Contractin A と命名された．Contractin A はモルモット気管平滑筋においてホスホリパーゼC様の作用を示した．Contractin A のN末端はセリンで，37残基までアミノ酸配列分析がされた． (Keyword) Sea urchin / Pedicellarial venom / Toxopneustes pileolus / Tracheal smooth muscle / Purification / Arachidonic acid
27.	Hideyuki Nakagawa, Miller A. Roger, Tu T. Anthony, Toshiaki Nikai and Hisayoshi Sugihara : Partial amino acid squence of hemorrhagic toxin, Ac1 protease, isolated from snake venom of Agkistrodon acutus, Biochemical Archives, Vol.5, No.3, 243-247, 1989. (Summary) 毒蛇ヒャッポダの毒から，亜鉛を含有する出血因子としてAc1-プロティナーゼを精製した．Ac1-プロティナーぜの部分アミノ酸配列決定は，HPLC で精製された標品および還元カルボキシメチル化された標品をエドマン分解法で行った．Ac1-プロティナーゼのN 末端はセリンで，アミノ酸残基39個が明らかになった． (Keyword) snake venom / Agkistrodon acutus / proteinase / partial amino acid sequence
28.	Hideyuki Nakagawa, Motoo Oka, Akira Kimura and Takeshi Ohuchi : Effect of Age on the formation of cyclic nucleotides in gunea-pig tracheal smooth muscle in response to pharmacological agents, European Journal of Pharmacology, Vol.125, No.2, 211-216, 1986. (Summary) 幼若および老齢モルモット気管平滑筋において，平滑筋弛緩薬によるcAMP生成および平滑筋収縮薬によるcGMP生成の加齢にともなう変化を比較した．イソプロテレノールやプロスタグランジン，コレラトキシン等によるcAMP生成は，幼若モルモット気管平滑筋に比べて老齢モルモット気管平滑筋において減少した．一方，ヒスタミンや高濃度カリウムによるcGMP生成は，cAMP生成とは逆に老齢モルモット気管平滑筋で増大した． (Keyword) age / cyclic nucleotide / guinea-pig / tracheal smooth muscle
29.	Hitoshi Houchi, Hideyuki Nakagawa, Motoo Oka, Joe Watanabe, Minoru Isosaki and Takeshi Ohuchi : Incease in cyclic AMP level and stimulation of catecholamine synthesis in isolated bovine adrenal medullary cells caused by forskolin, Journal of Pharmacological Sciences, Vol.36, No.1, 97-99, 1984. (Summary) ウシ副腎髄質の浮遊細胞を用いて，アデニル酸シクラーゼを活性化するフォルスコリンの細胞内cAMP レベルに対する影響および14C-チロシンから14C-カテコラミン生合成に対する影響について検討した．フォルスコリンによって細胞内cAMPレベルは著明に上昇し，14C-カテコラミン生合成の促進が認められた． (Keyword) Bovine adrenal medullary cells / Forskolin / Catecholamine synthesis / Cyclic AMP (Link to Publication Site) ● Publication site (DOI): 10.1254/jjp.36.97 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1254/jjp.36.97 (DOI: 10.1254/jjp.36.97)
30.	Akira Kimura, Hideyuki Nakagawa, Hiromi Hayashi and Koichi Endo : Seasonal changes in contractile activity of a toxic substance from the pedicellariae of the sea urchin Toxopneustes pileolus, Toxicon, Vol.22, No.3, 353-358, 1984. (Summary) ラッパウニの叉棘タンパク毒の季節変化を摘出モルモット回腸縦走筋の収縮活性を指標として検討した．ウニ叉棘の抽出標品のタンパク量は春から夏にかけて増加したが，活性とは平行しなかった．また，タンパク量と活性の変化はウニの生殖期とも密接に関連しなかった．ゲル濾過画分の収縮活性もタンパク量の変化と平行せず，夏に低く秋から冬に増加した．これらの結果より，ラッパウニの叉棘タンパク毒の季節的変動が示唆された． (Keyword) Seasonal change / Sea urchin / Toxopneustes pileolus / Toxic substance / Contractile activity / Protein content (Link to Publication Site) ● Publication site (DOI): 10.1016/0041-0101(84)90079-5 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 6474489 ● Summary page in Scopus @ Elsevier: 2-s2.0-0021198905 (DOI: 10.1016/0041-0101(84)90079-5, PubMed: 6474489, Elsevier: Scopus)
31.	Hideyuki Nakagawa and Akira Kimura : Partial purification and characterization of a toxic substance from pedicellariae of the sea urchin Toxopneustes pileolus, The Japanese Journal of Pharmacology, Vol.32, No.5, 966-968, 1982. (Summary) ラッパウニの叉棘粗抽出液の硫安分画をセファデックスG-200カラムでゲル濾過した結果，二つのタンパク質画分が回収された．この内，収縮活性を有する画分をPeak-II画分とした．Peak-II画分の比活性は粗抽出液に比して5.8倍に上昇した．Peak-II画分は硫安画分より低濃度で肥満細胞からヒスタミン遊離を起した．Peak-II画分の分子量は約20,000と推定された． (Keyword) partial purification / a toxic substance / sea urchin / Toxopneustes pileolus / sea urchin toxin (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7176229 ● Summary page in Scopus @ Elsevier: 2-s2.0-0019967396 (PubMed: 7176229, Elsevier: Scopus)
32.	Hideyuki Nakagawa, Akira Kimura, Masao Takei and Koichi Endo : Histamine release from rat mast cells induced by an extract from the sea urchin Toxopneustes pileolus, Toxicon, Vol.20, No.6, 1095-1097, 1982. (Summary) The extract caused a dose-dependent release of histamine from rat peritoneal mast cells which was slower in onset than that of compound 48/80. It is also suggested that the extract-induced histamine release is dependent on aerobic glycolysis. (Keyword) Sea urchin / Toxopneustes pileolus / Mast cells / Histamine release / Compound 48/80 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 6187106 ● Summary page in Scopus @ Elsevier: 2-s2.0-0020465466 (PubMed: 6187106, Elsevier: Scopus)
33.	Tetsuyuki Nasu, Kazumi Yui, Hideyuki Nakagawa and Yukio Ishida : Role of glycolysis in the tension development under anoxia in guinea pig teania coli, The Japanese Journal of Pharmacology, Vol.32, No.1, 65-71, 1982. (Summary) The role of glycolysis in the tension development under anoxic conditions in a high-K medium was studied in the intestinal smooth muscle of guinea pig taenia coli. After exposure to the high-K medium (isotonic, 60 mM) under normal oxygen for 30 min, the muscles were exposed to a high-K medium bubbled with N2 gas. The tonic contraction decreased gradually to about 10% of the original level. Glucose was then cumulatively added to the high-K medium under anoxia. The maximum tension was observed following the addition of the higher concentrations of glucose. The muscle tension which developed in the high-K medium with a high concentration of glucose under anoxia was dependent on the external Ca2+ and was inhibited by iodoacetic acid (IAA). The addition of glucose to a high-K medium under anoxia also increased lactate release from the muscle. Pretreatment with 1 mM IAA decreased the lactate release from the muscle. In a Ca2+-free medium under anoxia, the addition of glucose did not increase the muscle tension although there was a significant increase in the lactate release. In summary, it is considered that the smooth muscle of taenia coli develops tension utilizing energy produced by the glycolytic pathway under anoxia in a high-K medium. (Keyword) anoxia / gunea pig tania coli / tension development / glycolysis / adenosine triphosphate (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7087264 ● Summary page in Scopus @ Elsevier: 2-s2.0-0020041399 (PubMed: 7087264, Elsevier: Scopus)
34.	Akira Kimura and Hideyuki Nakagawa : Action of an extract from the sea urchin Toxopneustes pileolus on isolated smooth muscle, Toxicon, Vol.18, No.6, 689-693, 1980. (Summary) ラッパウニの叉棘抽出液の硫安分画によるモルモット摘出回腸縦走筋の収縮作用ならびにヒスタミン遊離作用について検討した．硫安文画による収縮作用は抗ヒスタミン薬によって部分的に抑制された．硫安分画は高濃度において有意にヒスタミン遊離を引き起こした．硫安分画によるモルモット摘出回腸縦走筋の収縮反応は部分的にヒスタミン遊離を介するものと思われた． (Keyword) sea urchin / Toxopneustes pileolus / longitudinal muscle / gunea pig ileum (Link to Publication Site) ● Publication site (DOI): 10.1016/0041-0101(80)90101-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 6164130 ● Summary page in Scopus @ Elsevier: 2-s2.0-0019214209 (DOI: 10.1016/0041-0101(80)90101-4, PubMed: 6164130, Elsevier: Scopus)
35.	Shigeru Shimomura, Akira Kimura, Hideyuki Nakagawa and Masaomi Takao : Mercury levels in human hair and sex factors, Enviromental Resaerch, Vol.22, No.1, 22--30, 1980. (Summary) 四国居住の人(N=1324)の毛髪中の水銀量を分析した．水銀量は幾何平均値で示し，その値を対数確率で表すと，正規分布型であった．男性の値は女性より高値を示した．農村地区の中学生(N=300)および成人(N=354)の分析において，男性の値は両者共に女性よりも高値を示した．これらの結果は，体内の水銀量に内分泌系が関与することを示唆した． (Keyword) Mercury level / Human hair / sex
36.	Aritomo Suzuki, Noriyoshi Kajimoto and Hideyuki Nakagawa : Contractile mechanism of ATP in isolated vas deferens of guinea pig, Acta Medica Kinki, Vol.1, 59-62, 1976. (Summary) モルモット摘出精管において，ATPとADPは収縮を引き起こしたが，AMPとアデノシンは収縮を起こさなかった．ATPの収縮反応は一過性であり，膜電位の変化と平行した．この収縮反応はアトロピンあるいはフェントールアミンによって若干抑制を受けた．以上の結果から，精管に対してATPは一部神経要素に作用するが，大部直接平滑筋に作用するものと考えられる． (Keyword) guinea pig vas deferens / ATP / contractile mechanism
37.	Hideyuki Nakagawa, Tetsuyuki Nasu and Yukio Ishida : Effect of anoxia on isotonic shortening induced by high-K medium in rat uterus, The Japanese Journal of Pharmacology, Vol.26, No.3, 353-357, 1976. (Summary) The effect of anoxia on the shortening induced by high-K medium was investigated in isolated rat uterus. The shortening induced by high-K medium was maintained for 75 min or more under normoxia in the initial load (0.35 g). Under normoxia 1, 2, 2.5, and 3 g load to the uterus reduced the maximum shortening induced by high-K medium to 93, 65, 18 and 3%, respectively. Under anoxia the high-K-induced shortening in the initial load was not so affected and it was maintained at least for 60 min. However, against 0.7 and 1 g load to the uterus, the maximum shortening reduced greatly to 14 and 13% under anoxia. Effects of anoxia on the shortenings induced by high-K medium in guinea pigs taenia coli and longitudinal muscle from ileum were also studied. Our observations support the theory that the shortening of the light loaded muscle under anoxia is maintained by only a little force and the muscle may not be so dependent on metabolic energy. (Keyword) rat uterus / isotonic shortening / anoxia / high-K medium (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 978848 ● Summary page in Scopus @ Elsevier: 2-s2.0-0017082553 (PubMed: 978848, Elsevier: Scopus)
38.	Yukio Ishida, Noriaki Kondo, Syunzo Aihara and Hideyuki Nakagawa : Difference between inhibitory actions of papaverine and dehydroepiandrosterone on the isolated guinea-pig ileum, The Japanese Journal of Pharmacology, Vol.25, No.6, 719-726, 1975. (Summary) Dehydroepiandrosterone (DEA), a metabolite of steroid hormone in the urine, differed from papaverine in the mode of inhibitory action on the isolated guinea-pig ileum. Papaverine inhibited both phasic and tonic contractions induced by an agonist, while DEA inhibited the phasic contraction without apparent fatigue of the tonic phase. The main action of papaverine seems to be predominatly a metabolic inhibition similar to that of metabolic inhibitors, but the action of DEA could not be ascribed to that of metabolic inhibition. In the presence of papaverine or metabolic inhibitors the height of maximal contraction of an agonist tested by the cumulative method was greatly depressed and less than that by a single method. The longer the time involved in the cumulative doses, the lower was the maximal contraction. (Keyword) guinea-pig ileum / papaverine / dehydroepiandrosterone / metabolic inhibition (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 132553 ● Summary page in Scopus @ Elsevier: 2-s2.0-0016676637 (PubMed: 132553, Elsevier: Scopus)

Academic Paper (Unrefereed Paper):

1.	Hideyuki Nakagawa, Fumihiko Satoh, Ambreena AmSiddiq, Hiromi Hayashi, Eiichi Tachikawa, Yasuhiro Tomihara and Yasutetsu Araki : ウニ刺毒レクチンの細胞反応性について, 第47回毒素シンポジュム予稿集, Vol.47, 86-93, 2000. (Summary) ラッパウニの大型叉棘レクチンの細胞増殖活性と細胞毒性について検討を行った． (Keyword) Sea urchin / Mitogenic activity / cytotoxicity
2.	Akira Kimura, Hideyuki Nakagawa and Akiko Kitahama : Physiological studies of spontaneous motor activity of mice, --- Circadian rhythm of spontaneous motor activity and influence of components of nonessential grocery items ---, J. Health Sci., Fac. of Integrated Arts and Sciences, Univ. of Tokushima, Vol.2, 53-69, 1990. (Summary) マウスの自発運動における日の出前後と日没とに見られる二峰性の日内リズムは，周年において雄よりも雌に強く認められた．そこで，マウス自発運動の日内リズムに対するエタノール，カフェインおよびニコチンの影響を検討した．エタノールおよびニコチン投与群では二峰性の自発運動のリズムには変化は認められなかったが，カフェイン投与群では二峰性のリズムは雌雄のマウス共に消失した． (Keyword) Mice / Spontaneous motor activity / Ethanol / Nicotine / Caffeine / Circadian rhythm
3.	Akira Kimura, Hideyuki Nakagawa and Akiko Kitahama : Physiological studies of spontaneous activity of mice, --- Circadian rhythm of spontaneous activity and influence of morphine ---, J. Health Sci., Fac. of Integrated Arts and Sciences, Univ. of Tokushima, Vol.2, 1-23, 1990. (Summary) マウスの自発運動における日内リズムに対する飼料摂取制限および薬物の影響を検討した．マウス自発運動は昼間よりも夜間の飼料摂取制限によって著明に減少した．モルヒネ投与による自発運動の日内リズムの変化は雄よりも雌マウスに著しく認められた． (Keyword) Mice / Circadian rhthm / Spontaneous activity / Morphine
4.	Hideyuki Nakagawa and Akira Kimura : Histamine H2-receptors in isolated ginea-pig airway smooth muscles, Commemoration Volume of the Founding of the Faculty of Integrated Arts and Sciences, University of Tokushima, 443-449, 1987. (Summary) 幼若モルモット気道平滑筋(気管および気管支平滑筋)におけるヒスタミンH2受容体の発現について，等尺性の収縮·弛緩反応を指標に検討した．気道平滑筋の緊張度が増大した場合には，ヒスタミンアゴニストのH2受容体刺激による弛緩反応が認められ，またこの弛緩反応がH1アンタゴニストで増強され，逆にH2アンタゴニストで減弱した．この結果から，幼若モルモット気道平滑筋にはヒスタミンH2受容体が発現していることが推定された． (Keyword) Guinea-pig / Tracheal smooth muscle / Histamine H2-receptor / Histamine H2 agonist
5.	Hideyuki Nakagawa : Age-dependent alterations in the formation of cyclic nucleotides caused by various agents in guinea-pig tracheal smooth muscle, The Tokushima Journal of Experimental Medicine, Vol.30, No.3/4, 85-91, 1983. (Summary) モルモット気管平滑筋の加齢に伴う薬物反応性の変化を検討した．イソプロテレノールやプロスタグランジンなどによるcAMPの生成は加齢により減少した．一方，カルバコールやヒスタミンなどによるcGMPの生成はcAMPの場合とは逆に増大した．放射性リガンドを用いた受容体の結合実験では加齢による変化は見られなかった．以上より，加齢に伴うcAMPおよびcGMP生成の変化は，モルモット気管平滑筋の受容体以後の細胞内レベルによるものと考えられた． (Keyword) age / guinea-pig / cyclic nucleotides / tracheal smooth muscle / binding assay
6.	Takao Yanagawa, Aritomo Suzuki, Takashi Tajiri and Hideyuki Nakagawa : Influence of imidazole on the response to the smooth muscle relaxants, Med. J. Kinki Univ., Vol.4, No.4, 553-558, 1979. (Summary) ウサギ摘出小腸を用い，諸種薬物の作用とcAMPとの関連性をimidazoleの影響から検討した．α受容体刺激薬，NaNO2およびATPによる弛緩作用はimidozoleにより抑制されなかったが，β受容体刺激薬，dibutryl cAMPあるいは低濃度のpapaverineによる弛緩はimidazoleにより著明に抑制された．これらの結果から，細胞内cAMPを介する弛緩作用はimidazoleによって抑制されると示唆された． (Keyword) rabbit ileum / imidazole / cyclic AMP / smooth muscle relaxant
7.	Akira Kimura and Hideyuki Nakagawa : Effects of isoproterenol and propranolol on estrous cycle through pineal mechanisim in developmental stage of genital organs, Nat. Sci, Fac. of Education, Univ. of Tokushima, Vol.18, No.6, 43-46, 1979. (Summary) ラットの発情周期に対する交感神経作用薬及び遮断薬の影響を検討した．交感神経作用薬の投与群は，対照群に比べて不規則な発情期の増加を示した．一方，交感神経遮断薬の投与群では，不規則な発情期の増加を起こしたが，交感神経作用薬の場合とは逆に持続的な発情期も増加させた． (Keyword) Rat / Isoproterenol / Propranolo / Estrous cycle / Genital organs
8.	Aritomo Suzuki, Sumiko Gamou and Hideyuki Nakagawa : Mechanism of the contracting actions of K, acetylcholine, noradrenaline and Ba in the excised seminal vesicle of guinea pig, particulary in relation to Ca., Med. J. Kinki Univ., Vol.1, No.2, 115-120, 1976. (Summary) モルモット摘出精嚢条片におけるK+, acetylcholine(ACh), noradrenaline (NA)およびBa2+による収縮はphasic contraction (PC)とこれに続くtonic contraction(TC)よりなる．AChおよびNAのPCならびにTcは主にCa2+の遊離によって起こるが，K+のPCはCa2+の遊離と流入によって維持されると推定された．Ba2+の収縮はCa2+の遊離のみによって起こるものと推定された． (Keyword) guinea pig seminal vesicle / potassyuim / acetylcholine / noradrenaline / calcium / contraction

Academic Letter:

1.	Hisao Kamiya, Kazuo Shiomi and Hideyuki Nakagawa : Current Topics on Toxins of Stinging Marine Animals, Nippon Suisan Gakkaishi, Vol.69, No.5, 824, 2003. (Summary) 海洋刺毒動物によるこれまでの被害状況と一部刺毒に関する最近の化学的知見をまとめた． (Keyword) venomous marine animals / box jellyfishes / sea anemone / starfish / redfin velvetfish (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1542543045206083072 (CiNii: 1542543045206083072)
2.	Hideyuki Nakagawa, Akira Kimura, Masao Takei and Kouichi Endo : レクチンカラムによるラッパウニ叉棘糖タンパク質の分画とその作用, 日本平滑筋誌, Vol.23, No.5, 406-408, 1987. (Summary) ラッパウニ叉棘粗抽出液のゲル濾過画分をConcanavalin A-Sepharose カラムにより分画し，活性画分として回収したCon A 画分を用いてモルモット気管平滑筋に対する作用を検討した．糖蛋白質画分であるCon A 画分はモルモット気管平滑筋においてアラキドン酸の場合と同様な反応性を示し，筋の緊張度によって収縮あるいは弛緩反応を示した．Con A 画分による反応はプロスタグランディン産生を介するものと推定された． (Keyword) Toxopneustes pileolus / Sea urchin / Affinity chromatography / Prostaglandin / Arachidonic acid
3.	Hideyuki Nakagawa and Akira Kimura : ラッパウニ毒成分のゲル濾過法による精製とその作用, Journal of Smooth Muscle Research, Vol.15, No.2, 126-128, 1979. (Summary) ラッパウニ叉棘粗抽出液の硫安画分をゲル濾過で分画した結果，二つの画分が回収された．このうち，モルモット回腸縦走筋において収縮活性を示す画分(P-II画分)のモルモット気管平滑筋に対する作用を検討した．P-II 画分はATPと同様な筋の静止張力に依存して収縮あるいは弛緩反応を示した．P-II 画分による両反応は Indomethacin の処理により消失した． (Keyword) Toxopneustes pilelous / Sea urchin / Venom / Guinea-pig tracheal smooth muscle / Indomethacin
4.	Hideyuki Nakagawa, Sumiko Gamou, Noriyoshi Kajimoto and Aritomo Suzuki : 諸種収縮薬の収縮作用におよぼすウワバインの影響，特にNa, K-ATPase活性阻害との関連について, Journal of Smooth Muscle Research, Vol.12, No.4, 217-219, 1976. (Summary) モルモット摘出精嚢の収縮作用に対するウワバインの影響をNa, K-ATPase活性阻害との関連から検討した．Na, K-ATPase活性を阻害しない低濃度のウワバインは諸種収縮薬の収縮作用を増強したが，ATPase活性を抑制する濃度では各収縮作用を抑制した．このことから，収縮抑制はNa. K-ATPase活性の阻害と関係があるものと推定された． (Keyword) Guinea-pig seminal vesicle / Ouabain / Na, K-ATPase / Contraction

Review, Commentary:

1.	Hideyuki Nakagawa : ウニ類の棘には毒がある, Biophilia, Vol.5, No.1, 14-19, Mar. 2009. (Keyword) sea urchin venom
2.	Hideyuki Nakagawa : 海洋資源を利用した医薬開発の最近の現状, Farumashia, Vol.32, No.6, 694-695, Jun. 1996. (Summary) 海洋生物からの新薬をめだす最近の動向を，パテントの獲得，新しいアプローチの導入や生物資源の保護などの観点から解説した． (Keyword) Lead compund / Marine sources / Patent / High-truoughput screening / Drug discovery
3.	Tu T. Anthony and Hideyuki Nakagawa : 続·身のまわりの毒, Chemistry Today, 53-55, Aug. 1990. (Summary) ウニとナマコの毒の性質および治療について解説した． (Keyword) sea urchin venom / sea cucumber venom / therapy

Proceeding of International Conference:

1.	Hideyuki Nakagawa, Kozue Edo, Hitomi Sakai, Yuika Watanabe, Mitsuko Shinohara and Kiyoshi Ohura : Mining on novel lectins from some venomous fish and sea urchins, Abstract of 9th IST Asia Pacific Meeting on Animal, Plant and Microbial Toxins, 10, Vladivostok, Russia, Sep. 2011. (Keyword) D-galactose-binding lectin / venomous fish / sea urchin venom / Karatoxin / dendritic cell
2.	Kiyoshi Ohura, Mitsuko Shinohara, Hideyuki Nakagawa and Kozue Edo : A novel chemoattractant protein, Karatoxin, from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, XX1st International Symposium on Medicinal Chemistry, Vol.21, 124, Copenhagen, Sep. 2010. (Keyword) velvetfish / chemotaxis / galectin-3
3.	Natsuko Kusunoki, Kozue Edo, Kaori Ohnishi, Keiko Ashida, Asami Nakanishi, Hitomi Sakai, Hideyuki Nakagawa, Kumio Yokoigawa, Haruhiko Sakuraba and Toshihisa Ohshima : Multiple lectins from globiferous pedicellariae of the sea urchin, Toxopneustes pileolus, The Proceeding of 21st Pacific Science Congress:Session:4-10 Health hazard by marine animals in Pacific region, 7p, Okinawa, Jun. 2007. (Keyword) lectin / sea urchin / venom / Toxopneustes pileolus / pedicellariae
4.	Hideyuki Nakagawa, Fumihiko Satoh, Takefumi Hosotani, Kozue Edo, Yasutetsu Araki, Masatoshi Nozaki and Yasuhiro Tomihara : Biologically Active Substances from Venomous Scorpionfish, The proceeding of 21st Pacific Science Congress: Session: 4-10 Health hazard by marine animals in Pacific region, 4p, Okinawa, Jun. 2007. (Keyword) scorpionfish / dorsal venom / Hypodytes rubripinnis / Inimicus japonicus / Synanceia verrucosa
5.	Hideyuki Nakagawa : Isolation of a novel lectin from the dorsal venom of the redfin velvetfish, Hypodytes rubripinnis, Gordon Research Conference on Marine Natural Products, Ventura, California, Feb. 2006. (Summary) ハオコゼの刺棘タンパク質画分よりレクチンを精製した．レクチンの分子量は10万で，それぞれ8万，4万，1，5万のサブユニットの部分アミノ酸配列を分析した．8万ユニットのアミノ酸配列の結果は一部，タイコブラの毒素の配列と一致した． (Keyword) Hypodytes rubripinnis / Toxin / Venomus fish / Lectin / Purification
6.	Hideyuki Nakagawa, Hitomi Sakai, Kaori Kanemaru and Hiromi Hayashi : A sea urchin lectin, SUL-I induces differentiation on murine myeloid leukemic cells, GRC on Marine Natural Products, Ventura, California, Feb. 2004. (Summary) ラッパウニ叉棘から精製されたD-galactose binding lectin のSUL-I はマウス由来のmyeloid leikemic cell をマクロファージ様に分化誘導し，部分的なアポトーシスを引き起こした． (Keyword) murine myeloid cells / differentiation / D-galatose-binding lectin / sea urchin lectin / Toxopneustes pileolus
7.	Hideyuki Nakagawa, Takahiko Tanigawa, Katsuyuki Tomita, Yasuhiro Tomihara, Yasutetsu Araki and Eiich Tachikawa : Recent studies in sea urchin venoms, International Symposium on Toxins and Natural Products in Honor of Professor Anthony T. Tu, Naha, Nov. 2002. (Summary) We described mainly the isolations oand characterizatons of sea urchin lectins from the venoms and coelomic fluid of the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla. (Keyword) sea urchin venoms / lectin / D-galactose / amino acid / bioactive substance
8.	Hideyuki Nakagawa, Fumihiko Satoh, Hitomi Sakai, Hiromi Hayashi and Yasuhiro Ozeki : Biological activities of multiple lectins from the toxopneustid sea urchins, 10th Pacific Congress on Marine Science and Technology, Chiba, Jun. 2002. (Summary) We isolated two D-galactose-binding lectins (SUL-I and SUL-II) and a heparin-binding lectin (TGL-I) from Toxopneustes pileolus and Tripneustes gratilla, respectively. (Keyword) toxopneustid sea urchins / lectins / D-galactose / mitogenic activity / chemotaxis
9.	Fumihiko Satoh, Hideyuki Nakagawa, Kuniko Nagasaka, Toshinori Nagasaka, Haruhiko Sakuraba and Toshihisa Ohshima : Fishing for Bioactive Compounds from Some Marine Vertebrate and Invertebrates, 7th Eurasia Conference on Chemical Sciences, Karachi, Pakistan, Mar. 2002. (Summary) We suggested that somespecies of scorpionfish and the toxopneustid sea urchins may be a resource for biologically active substances. (Keyword) scorpionfish / toxoneustid sea urchins / mitogenic activity / cytotoxicity
10.	Hideyuki Nakagawa, Fumihiko Satoh, Kuniko Nagasaka and Toshinori Nagasaka : Comparative study of biological activity of venom proteins fron the redfin velvetfish Hypodytes rubripinnis and stonefish Synanceia verrucosa, Gordon research Conferences on Marine Natural Products, Ventura, California, Feb. 2002. (Summary) We compared the biological activities of venom proteins from the dorsal spines of H. rubripinnis and S. verrucosa. (Keyword) redfin velvetfish / stonefish / dorsal spines / mitogenic activity / cytotoxicity
11.	Fumiaki Satoh, Hideyuki Nakagawa, Yasutetsu Araki, Yasuhiro Tomihara, Kuniko Nagasaka and Toshinori Nagasaka : Comparative study of venom proteins from the species of scorpionfish, Hypodytes rubripinnis and Synanceia verrucosa, 10th International Symposium on Marine Natural Products, 151, Okinawa, Jun. 2001. (Summary) ハオコゼとオニダルマオコゼの背鰭毒棘のタンパク質成分と生物活性を比較検討した．両者のタンパク質成分は類似しており，細胞毒性と細胞増殖活性の活性の度合いも似ていた． (Keyword) scorpionfish / Hypodytes rubripinnis / Synanceia verrucosa / dorsal spine / venom protein
12.	Hideyuki Nakagawa : Functional characterization of multiple lectins from the toxopneustid sea urchins, Gordon Research Conferences on Marine Natural Products 2000, Ventura, CA, Feb. 2000. (Summary) ラッパウニ科のラッパウニおよびシラヒゲウニからのレクチンの生物活性および構造について検討した． (Keyword) Toxopneustes pileolus / Tripneustes gratilla / SUL-I / SUL-II / TGL-I / amino acid
13.	Hideyuki Nakagawa, Hitomi Sakai, Hiromi Hayashi, Yasuhiro Tomihara and Yasutestsu Araki : Some physiological studies of pedicellarial lectins from the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla, 6th International Symposium: Protein Structure Function Relationship, Karachi, Pakistan, Feb. 1999. (Summary) ラッパウニ科のラッパウニおよびシラヒゲウニの叉棘からレクチンを精製し，生物活性について比較検討した． (Keyword) Toxopneustes pileolus / Tripneustes gratilla / Pdeicellariae / Mitogenic activity
14.	Hideyuki Nakagawa and Chieko Yamaguchi : Cytostatic effect of a β-galactoside-binding lectin fron the sea urchin, Toxopneustes pileolus, The 9 th Naito Conference on Bioactive Natural Products and their Modes of Action, 104, Tokyo, Oct. 1997. (Summary) ラッパウニの大型叉棘から精製されたレクチン(SUL-I)はマウス脾臓細胞の増殖活性を示したが，一方，増殖の抑制作用も引き起こした．これらの作用はSUL-I が細胞膜上のDーガラクトスとの結合によるものであると思われた． (Keyword) sea urchin lectin / Toxopneustes pileolus / D-galactose / mitogenic stimulation / cytostaitic effect
15.	Hideyuki Nakagawa, Chieko Yamaguchi, Fukiyo Tomiyoshi and Hiromi Hayashi : A novel lectin from the globiferous pedicellariae of sea urchin, Toxopneustes pileolus, 5 th International Symposium : Protein Structure Function Relationship, 34, Karachi, Jan. 1997. (Summary) ラッパウニの大型叉棘から，新規レクチンとして294アミノ酸残基からなる分子量3.2万のタンパク質(SUL-I)を精製した．SUL-IのN 末端はアラニンで37アミノ酸残基まで分析された． (Keyword) Sea urchin / Toxoneustes pileolus / D-galacotose binding lectin / Amino acid residue
16.	Hideyuki Nakagawa, Chieko Yamaguchi and Hiromi Hayashi : Biologically active substances from sea urchins, 13 th Rocky Mountain Regional Meeting, Denver, Jun. 1996. (Summary) 4種類の有毒種のウニからレクチンの探索を試みた．この内，ラッパウニの大型叉棘から，分子量3.2万と2.3万のD-ガラクトース結合性のレクチンを精製し，それぞれSUL-I およびSUL-II と命名した．SUL-I はモルモット好中球やマクロファジに対して遊走活性を示した．ウニの毒棘蛋白質成分から，生体に有用な生理活性物質の利用が示唆された． (Keyword) sea urchins / Toxopneustes pileolus / Tripneustes gratilla / Diadema setosum / Asthenodoma sp. / lectin
17.	Hideyuki Nakagawa, Tamiko Hashimoto, Hiromi Hayashi, Mitsuko Shinohara, Kiyoshi Ohura and Eiichi Tachikawa : Isolation of novel lectins from the globiferous pedicellariae of the sea urchin Toxopneustes pileolus, 209th American Chemical Society National Meeting, 77, Anaheim, CA, Apr. 1995. (Summary) The pedicellariae of globiferous type from the sea urchin, Toxopneustes pileolus contain biologically active substances and cause serious local and systemic effects. In the present study, we aimed to isolate novel lectins from the globiferous pedicellariae and large globiferous pedicellariae of T. pileolus. The lectin-like protein fractions from the pedicellariae of the two sub-types were fractionated by gel chromatography. Two lectins were purified bu affinity chromatography on an immobilized D-galactose or immobilized lactose. On SDS-PAGE, the molecular mass of both lectins was estimatd to be about 32,000 and 18,000, respectively. These lectins may play a role in defense. (Keyword) Sea urchin / Toxopneustes pileolus / Lectin / Globiferous peidicellaria / Isolation
18.	Masao Takei, Koichi Endo, Hideyuki Nakagawa and Akira Kimura : Anticholinergic action of a pedicellarial gycoprotein from the sea urchin Toxopneustes pileolus, Progress in Venom and Toxin Research, 464-466, Singapore, Nov. 1987. (Summary) ラッパウニの叉棘糖蛋白質画分(Con A 画分) はレクチンアフィニティクロマトグラフィーにより分画された．Con A 画分はアセチルコリンによるカエル腹直筋の収縮反応を抑制したが，カリウムによる収縮反応には無影響であった．アセチルコリンエステラーゼ阻害薬の存在でCon A 画分のアセチルコリン収縮の抑制作用は消失した． (Keyword) Acetylcholine / Pedicellarial glycoprotein / Frog rectus abdominis / Toxopneustes pileolus / sea urchin
19.	Hideyuki Nakagawa, Akira Kimura, Masao Takei and Koichi Endo : Sea urchin Toxin-induced degranulation of rat mesentery mast cells, Toxicon, Newcastle upon Tyne, Aug. 1985.

Proceeding of Domestic Conference:

1.	畠山智充, 東絵梨花, 海野英昭, 郷田秀一郎 and Hideyuki Nakagawa : ラッパウニ毒素タンパク質 Contractin A の cDNAクローニングと大腸菌による発現, 日本農芸化学会2015年度大会(岡山)プログラム集, 103, Mar. 2015. (Keyword) Toxoneustes pileolus / toxin / cDNA subtractive screening / クローニング / フォスホリパーゼ
2.	Hitomi Sakai, Kozue Edo, Maoko Yoshino, Hideyuki Nakagawa, Mitsuko Shinohara and Kiyoshi Ohura : Isolation of novel lectins from the globiferous pedicellariae of the toxopneustes sea urchin, Toxopneustes pileolus, 88th JPS Annual Meeting Program, 214p, Feb. 2015. (Keyword) sea urchin / venom / lectin / mitogen activity / agglutinating activity
3.	Edo Kozue, Hitomi Sakai, Yoshino Maoko and Hideyuki Nakagawa : 徳島産ラッパウニの叉棘に由来するレクチンの多様性について, 日本薬理学会西南部会プログラム・抄録集, 52, Nov. 2014. (Keyword) sea urchin / pedicellaria / venom / lectin / diversity
4.	一瀬彩香, 海野英昭, Shuichiro Goda, Hideyuki Nakagawa and 畠山智充 : ラッパウニ叉棘由来ガラクトース特異的レクチンSUL-Iの遺伝子クローニングと機能解析, 第87回日本生化学大会要旨集, 303, Oct. 2014. (Keyword) ラッパウニ / SUL-I / レクチン / クローニング / 血球凝集反応
5.	江戸梢, Hitomi Sakai, Hideyuki Nakagawa, Naoyoshi Nishibori and 西尾幸郎 : 徳島産ラッパウニの叉棘レクチンの生理活性について, 日本農芸化学会2014年度中四国支部大会(第40回公演会)講演要旨集, 49, Sep. 2014. (Keyword) ラッパウニ / ウニ毒 / レクチン / 凝集反応 / マイトジェン活性
6.	Hitomi Sakai, Kozue Edo, Hiroyuki Hase, Hideyuki Nakagawa, Mitsuko Shinohara and Kiyoshi Ohura : Isolation of a L-rhamnose-binding lectin from the globiferous pedicellariae of the toxopneustid sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.124, No.Suppl, 204P, Jan. 2014. (Keyword) sea urchin / Toxopnesustes pileolus / venom / lectin / L-rhmnose-biding lectin
7.	Hideyuki Nakagawa, Kozue Edo, Yuri Kotake, Hitomi Sakai, Mitsuko Shinohara and Kiyoshi Ohura : Isolation of a novel mitogenic factor from the pedicellariae of the toxopneustid sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.121, No.Supplement 1, 157P, Mar. 2013. (Keyword) sea urchin / Toxopnesustes pileolus / mitogenic activity / mitogen / venom
8.	Yuika Watanabe, Hideyuki Nakagawa, Kuniko Nagasaka, Mitsuko Shinohara and Kiyoshi Ohura : オニオコゼの背鰭刺毒レクチンの部分精製, Folia Pharmacologica Japonica, Vol.140, No.4, 9P, Oct. 2012. (Keyword) Inimicus japnicus / dorsal spine / poisoning / lectin / chemotaxis
9.	Hitomi Sakai, Hideyuki Nakagawa, kouzue Edo, Mitsuko Shinohara and Kiyoshi Ohura : ラッパウニの卵巣に由来するレクチンの探索, Folia Pharmacologica Japonica, Vol.140, No.4, 9P, Oct. 2012. (Keyword) sea urchin / Toxopneustes pileolus / ovary / bioactive substance / lectin
10.	Kozue Edo, Hitomi Sakai and Hideyuki Nakagawa : 徳島産ラッパウニの大型叉棘に由来するマイトジェン様物質の精製について, (社)日本家政学会中国・四国支部研究発表要旨集 2012, 9, Oct. 2012. (Keyword) sea urchin / Toxopneustes pileolus / mitogen / aimon acid sequence / chemotaxis
11.	Kozue Edo, Shizuka Satoh, Hitomi Sakai and Hideyuki Nakagawa : Diversity of bioactive substances from the globiferous pedicellariae of the sea urchin, Toxopneustes pileolus, 社団法人日本家政学会第64回大会研究発表要旨集, 119, May 2012. (Keyword) Toxopneustes pileolus / bioactive substance / galectin-3 / mitogen / sea urchin lectin
12.	Hideyuki Nakagawa, Jun Kurochi, Kozue Edo, Yuika Watanabe, Hitomi Sakai, Ayaka Miki, Shun Morikawa, Mitsuko Shinohara and Kiyoshi Ohura : Partial purification and characterization of a glycoprotein from the coelomic fluid of the sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.118, No.Suppl. 1, 258p, Feb. 2012. (Keyword) coelomic fluid / galectin-3 / Toxopneustes pileolus / agglunination / mitogenic response
13.	江戸梢, 橋本多美子, Hitomi Sakai and Hideyuki Nakagawa : 徳島産ラッパウニの叉棘に由来するレクチンのサイトカイン産生と遊走活性, 第58会(社)日本家政学会中国・四国支部研究発表会研究発表要旨集, 12, Oct. 2011. (Keyword) sea urchin / lectin / chemotaxis / inflammatory cytokine / pedicellaria
14.	Kozue Edo, Tamiko Hashimoto, Hitomi Sakai and Hideyuki Nakagawa : Biological activity and diversity of the pedicellarial lectin from the toxopneustid sea urchin, Toxopneustes pileoluse, (社)日本家政学会第63回大会研究発表要旨集, 141, May 2011. (Keyword) sea urchin / lectin / pedicellariae / diversity / biological activity / sea urchin venom
15.	Hideyuki Nakagawa, kozue Edo, Yuika Watanabe, Hitomi Sakai, Mitsuko Shinohara and Kiyoshi Ohura : Biological activites of a novel lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, Journal of Pharmacological Sciences, Vol.115, No.Suppl. 1, 231p, Mar. 2011. (Keyword) Hypodytes rubripinnis / Dorsal spine / galectin-3 / Mitogenic activity / Chemotactic activity
16.	Kozue Edo, Hideyuki Nakagawa, Hitomi Sakai, Tamiko Hashimoto, Jun Kurochi, Masahiro Sakata, Shizuka Satoh, Mitsuko Shinohara, Kiyoshi Ohura and Haruhiko Sakuraba : Biological activities of a novel glycoprotein, Karatoxin, from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, 第83回日本生化学会大会プログラム, 380, Dec. 2010. (Keyword) scorpionfish / redfin velvetfish / venom / lectin
17.	江戸梢, 橋本多美子, Hitomi Sakai and Hideyuki Nakagawa : ラッパウニ科のウニに由来する糖結合性タンパク質の性質について, 第57回日本家政学会中国・四国支部研究発表要旨集, 13, Oct. 2010. (Keyword) ウニ / レクチン / D-ガラクトース / 細胞増殖活性
18.	Hideyuki Nakagawa, Hitomi Sakai, Kozue Edo, Mitsuko Shinohara and Kiyoshi Ohura : Partial purification of lectin components of coelomic fluid from the sea urchin, Toxopneustes pileolus, Journal Pharamacological Sicences, Vol.112, No.Suppl. 1, 166, Feb. 2010. (Keyword) Coelomic fluid
19.	江戸梢, Hideyuki Nakagawa, 酒井仁美, 中西あさみ, 菅脇拓真, 櫻庭春彦, 篠原光子 and 大浦清 : ラッパウニの体腔液に由来するレクチンの部分精製, 生化学, Vol.81, No.9, 343, Sep. 2009. (Keyword) Toxopneustes pileolus
20.	Hideyuki Nakagawa, Kozue Edo, Hotomi Sakai, Takuma Sugawaki, Hiroshi Takae, Mitsuko Shinohara, Kiyoshi Ohura and Haruhiko Sakuraba : Isolation of a novel lectin from the sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.109, No.Suppl. 1, 142, Feb. 2009. (Keyword) sea urchin lectin
21.	篠原光子, Hideyuki Nakagawa and 大浦清 : オニオコゼの刺棘タンパク質成分からのレクチンの精製, Journal of Oral Biosciences, Vol.50, No.Suppl., 2004, Sep. 2008. (Keyword) Inimicus japanicus
22.	Hideyuki Nakagawa, Kozue Edo, Keiko Ashida, Asami Nakanishi, Hitomi Sakai, Kuniko Nagasaka and Haruhiko Sakuraba : Partial purification of dorsal lectins of the Japanese devil scorpionfish, Inimicus japonicus, Journal Pharamacological Sciences, Vol.106, No.Suupl. 1, 208, Feb. 2008. (Keyword) Inimicus japonicus
23.	河野直子, 細谷岳史, 宮田匡志, 萱野真弓, Hideyuki Nakagawa, Hitomi Sakai, Hiromi Hayashi, 野崎真敏, 篠原光子, 大浦清 and 長坂壽訓 : Partial purification of novel lectins from the dorsal spines of the reef stonefish, Synanceia verrucosa, Journal of Pharmacological Sciences, Vol.97, 278, Mar. 2005.
24.	Hideyuki Nakagawa, Takefumi Hosotani, Kuniko Nagasaka, Toshinori Nagasaka, Haruhiko Sakuraba and Toshihisa Ohshima : Isolation of a novel lectin from the dorsal venom of Japanese devil scorpionfish, Inimicus japonicus, 生化学, Vol.76, No.8, 1012, Aug. 2004. (Summary) 広島産オニオコゼの背鰭毒棘のタンパク質画分より，レクチンの精製を試みた．毒棘標品をアフィニティークロマトグラフィーにより分画·精製を行い，Dーマンノース結合性レクチンを分離した． (Keyword) Fish venom / Inimicus japonicus / Lectin / Dorsal spines / Mitogenic activity
25.	Hideyuki Nakagawa, Fumihiko Satoh, Yuuko Aguro, Takeshi Hosotani, Ai Yukata, Kaori Kanemaru, Kuniko Nagasaka, Toshinori Nagasaka, Mitsuko Shinohara, Tadashige Nozaki and Kiyoshi Ohura : Purification of a dorsal lectin from the redfin velvetfish, Hypodytes rubripinnis, Journal of Pharmacological Sciences, Vol.94, No.Supplement 1, 135p, Mar. 2004. (Summary) A novel lectin was isolated from the dorsal venom of H. rubripinnis by affinity chromatography and tested for mitogenicity. It showed a single protein band with a molecular mass of 120 kDa.
26.	Hideyuki Nakagawa : Venom protein from the redfin velvetfish, Hypodytes rubripinnis, Nippon Suisan Gakkaishi, Vol.69, No.5, 833-834, Sep. 2003. (Summary) ハオコゼの背鰭刺棘からのタンパク毒の精製ならびに生物活性について総説的な講演をおこなった． (Keyword) Hypodytes rubripinnis / Cytotoxic activity / Mitogenic activity / bioactive substance / Fish venom / Glycoprotein
27.	Hideyuki Nakagawa, Fumihiko Satoh, Hiroko Kagota, Koji Hiraga, Kuniko Nagasaka, Toshinori Nagasaka, Haruhiko Sakuraba and Toshihisa Ohshima : Isolation of a cytotoxic factor from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, Journal of Pharmacological Sciences, Vol.91, 130, Mar. 2003. (Summary) 小型刺毒魚であるハオコゼの背鰭毒棘のタンパク質画分より，細胞障害性を示す糖タンパク質をアフィニティークロマトグラフィーにより精製した，Native PAGE では分子量11万であった．SDS-PAGE では7.6 万，3.0 万，1 万数千の位置にそれぞれ，タンパク質バンドを呈した．7.6 万のサブユニットのN末端アミノ酸はアスパラギン酸であり，12残基のアスパラギン酸まで分析された． (Keyword) Hypodytes rubripinnis / Fish venom / Glycoprotein / Cytotoxic protein / Dorsal spine
28.	Fumihiko Satoh, Hideyuki Nakagawa, Haruo Yamada, Kuniko Nagasaka, Toshinori Nagasaka, Haruhiko Sakuraba and Toshihisa Ohshima : 刺毒魚ハオコゼの毒棘糖タンパク質の精製, 第75回日本生化学会大会, Vol.74, No.8, 727, Oct. 2002. (Summary) ハオコゼの背ビレ毒棘から糖タンパク質を精製した． (Keyword) redfin velvetfish / lectin / cytotoxicity / glycoprotein
29.	Fumihiko Satoh, Hideyuki Nakagawa, Hiromi Hayashi, Mitsuko Shinohara and Kiyoshi Ohura : Comparison of biological activities of venom proteins of the redfin velvetfish Hypodytes rubripinnis and the stonefish Synanceia verrucosa, 第75回日本薬理学会大会, Vol.88, 149, Mar. 2002. (Keyword) redfin velvetfish / stonefish / biological activity
30.	Kaori Kanemaru, Kuno Ryoko, Konishi Yukari, Sakai Hitomi, Yasuo Oyama, Hideyuki Nakagawa, Hiromi Hayashi and Katuhiko Noda : H2O2障害に対するヒジキの胸腺細胞保護作用, 日本食品科学工学会第48回大会, Sep. 2001.

Et cetera, Workshop:

1.	Hideyuki Nakagawa : Recent Study on Sea Urchin Venoms, Oct. 2004.
2.	Hideyuki Nakagawa : Recent Resarch on Sea Urchin Venoms, Mar. 2004. (Keyword) Biological activity / Lectins / Pedicellariae / Sea urchins / Venom / Toxins
3.	Hideyuki Nakagawa : 海胆と魚の毒, Ushio, No.532, 74-75, Jun. 2003. (Summary) ウニと魚のトゲからタンパク毒が精製された．生命の仕組みに関わる物質として期待される． (Keyword) Sea urchin toxin / Fish venom
4.	Hideyuki Nakagawa : Sea urchins and vanoumous sea urchins, 沖縄の海の安全を考える, 5, May 2000. (Summary) ウニをはじめとする海洋生物の毒は，生命の仕組みに関与する生命物質の可能性が考えられる． (Keyword) Marine organisms / Bioactive substance
5.	Hideyuki Nakagawa : ウニの棘から生理活性物質を探る, 徳島生物学会報, No.1, 8-9, Jun. 1998. (Summary) ウニ毒から生理活性物質を開発の可能性を検討した． (Keyword) Sea urchin / Lectin / Venom

Report:

1.	Ri-ichi Murakami, Toshihisa Ohshima, Kiyoshi Fukui, Hideyuki Nakagawa, Haruhiko Sakuraba and Daisuke Yonekura : ダイヤモンドなど炭素系薄膜を利用した新規高度耐久性バイオ基材の開発, 文部科学省科学研究費補助金(基盤研究(C)(2))成果報告書, Mar. 2004.
2.	Hideyuki Nakagawa, Chieko Yamaguchi, Hitomi Sakai and Hiromi Hayashi : 沖縄産ガンガゼの毒棘タンパク画分のマイトジェン活性について, 平成9年度海洋危険性生物対策事業報告書, 27-31, Naha, Oct. 1998. (Summary) ガンガゼ毒棘の抽出標品は強い血球凝集活性を示した．抽出標品をゲル濾過法で分画し，血球凝集活性と細胞増殖活性を測定した．細胞増殖活性は100倍上昇した．この画分による血球凝集反応はD-ガラクトサミンで弱い阻害を受けた．SDS-PAGE では同画分は20 kDa の位置にタンパクバンドを示した． (Keyword) Sea urchin / Diadema setosum / Lectin / Mitogenic activity / Toxin
3.	Hideyuki Nakagawa, 山口知栄子, Hitomi Sakai, Hiromi Hayashi, 富原靖博 and 新城安哲 : 沖縄産ウニ類の棘および叉棘タンパク画分のマイトジェン活性, 平成8年度海洋性危険生物対策事業報告書, 23-27, Naha, Mar. 1997. (Summary) 沖縄本島沿海で採集したシラヒゲウニ，ガンガゼおよびリュウキュウフクロウニの棘抽出蛋白質画分の血球凝集活性ならびに細胞増殖活性を検討した．ウサギ血液では，3種類ともに強い凝集活性を示した．この結果から，3種の抽出標品にはレクチンが存在することが推定された，そこで細胞増殖活性を検討した．シラヒゲウニ，ガンガゼおよびリュウキュウフクロウニ抽出標品は強い活性を示した．この結果は，3種類のウニは生理活性物質としてのレクチンの宝庫の可能性を示唆した． (Keyword) Lectin / Sea urchins / Tripneustes gratilla / Diadema setosum / Asthenodoma sp. / mitogenic activity

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

ダイヤモンドなど炭素系薄膜を利用した新規硬度耐久性バイオ基材の開発 (Project/Area Number: 14655047 )
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Professor Emeritus : Nakagawa, Hideyuki

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)