Tokushima University / Educator and Researcher Directory --- Watanabe, Minoru

Personal Web Page

https://researchmap.jp/minomino (researchmap)

Field of Study

○	Molecular Biology, Developmental Biology

Subject of Study

○	Molecular biological study of the early frog development (Xenopus laevis, Transcriptional Regulation, signal transduction, RNAi, development, morphogenesis, transcription factor, genome editing)

Book / Paper

Academic Paper (Judged Full Paper):

1.	Taisei Shiraki, Takuma Hayashi, Jotaro Ozue and Minoru Watanabe : Appropriate Amounts and Activity of the Wilms Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos, Journal of Developmental Biology, Vol.10, No.46, 2022. (Keyword) CRISPR/Cas9 / pronephros / transcription factor / Wilms tumor / WT1 / Xenopus laevis (Tokushima University Institutional Repository) ● Metadata: 117828 (Link to Publication Site) ● Publication site (DOI): 10.3390/jdb10040046 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36412640 ● Search Scopus @ Elsevier (PMID): 36412640 ● Search Scopus @ Elsevier (DOI): 10.3390/jdb10040046 (Tokushima University Institutional Repository: 117828, DOI: 10.3390/jdb10040046, PubMed: 36412640)
2.	Yuuta Tagami, Takeshi Nishiyama, Michiko Omote and Minoru Watanabe : Application of the RNA interference technique to Xenopus embryos: Specific reduction of the β-catenin gene products by short double-stranded RNA produced by recombinant human Dicer, Development Growth & Differentiation, Vol.63, No.9, 467-477, 2021. (Summary) RNA interference (RNAi) is a technique for suppressing the function of specific genes and is widely used in many organisms, including yeast, nematodes, flies, plants, mice, and cultured mammalian cells. As of date, this technique has not been successfully applied to Xenopus laevis embryos. In this study, we applied RNAi to Xenopus embryos using β-catenin as a model gene. Injection of long double-stranded RNA (dsRNA) corresponding to the 3'-untranslated region of β-catenin mRNA into embryos induced embryonic lethality without any specific phenotype. However, injection of short dsRNA, generated from long dsRNA by treatment with recombinant human Dicer, into embryos resulted in decreased expression of endogenous β-catenin mRNA and protein, as well as decreased Wnt signaling activity in the embryos. The decrease in β-catenin mRNA and protein levels was observed only after mid-blastula transition. Embryos injected with short dsRNA showed a characteristic phenotype of enlarged anterior structures and loss of posterior structures. These phenotypes, as well as the increased expression of the anterior gene and decreased expression of the posterior gene, suggest that RNAi against the β-catenin gene suppresses the "late Wnt signaling" involved in proper anterior-posterior patterning of Xenopus embryos. The effect of RNAi on Xenopus embryos was also found to be sensitive to temperature. These results strongly suggest that the RNAi technique can be applied to Xenopus embryos using short dsRNAs, appropriate temperature control, and proper selection of target genes. (Keyword) Xenopus laevis / β-catenin / RNAi / Wnt signaling / gene silencing (Link to Publication Site) ● Publication site (DOI): 10.1111/dgd.12762 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34817899 ● Search Scopus @ Elsevier (PMID): 34817899 ● Search Scopus @ Elsevier (DOI): 10.1111/dgd.12762 (DOI: 10.1111/dgd.12762, PubMed: 34817899)
3.	Satoshi Yoshitome, Yukito Aiba, Masahiro Yuge, Nobuaki Furuno, Minoru Watanabe and Nobushige Nakajo : Involvement of Myt1 kinase in the G2 phase of thefirst cell cycle inXenopus laevis, Biochemical and Biophysical Research Communications, Vol.515, No.1, 139-144, 2019. (Summary) During cleavage of Xenopus laevis, the first mitotic cell cycle immediately following fertilization is approximately 90 min and consists of S, G2, and M phases. In contrast, the subsequent eleven cell cycles are approximately 30 min and consist mostly of S and M phases. The balance between Cdc25 and Wee1A/Myt1 is thought to be crucial for Xenopus first cell cycle progression; however, the role of Myt1 in this period has not been fully investigated. In this study, we examined the roles of Myt1, Wee1A, and Cdc25A in the first cell cycle of Xenopus laevis. Inhibition of Cdc25A with antisense morpholino oligonucleotides lengthened the duration of the first cell cycle to some extent, whereas it was slightly shortened by ectopic Cdc25A expression, suggesting that the low concentration of Cdc25A during the first cell cycle does not fully account for the long duration of this cycle. Using the Wee1A antisense morpholino oligonucleotide and neutralizing antibody against Myt1, we found that Myt1 phosphorylates and inhibits Cdk1 much more effectively than Wee1A during the first cell cycle in Xenopus. Taken together, these results suggest that the activity of Myt1 is predominantly responsible for the duration of the long G2 phase in the first mitotic cell cycle in Xenopus. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2019.05.104 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31128913 ● Search Scopus @ Elsevier (PMID): 31128913 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2019.05.104 (DOI: 10.1016/j.bbrc.2019.05.104, PubMed: 31128913)
4.	Minoru Watanabe, Yuuri Yasuoka, Shuuji Mawaribuchi, Aya Kuretani, Michihiko Ito, Mariko Kondo, Haruki Ochi, Hajime Ogino, Akimasa Fukui, Masanori Taira and Tsutomu Kinoshita : Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis., Developmental Biology, Vol.426, No.2, 301-324, 2017. (Summary) Xenopus laevis has an allotetraploid genome of 3.1Gb, in contrast to the diploid genome of a closely related species, Xenopus tropicalis. Here, we identified 412 genes (189 homeolog pairs, one homeologous gene cluster pair, and 28 singletons) encoding transcription factors (TFs) in the X. laevis genome by comparing them with their orthologs from X. tropicalis. Those genes include the homeobox gene family (Mix/Bix, Lhx, Nkx, Paired, POU, and Vent), Sox, Fox, Pax, Dmrt, Hes, GATA, T-box, and some clock genes. Most homeolog pairs for TFs are retained in two X. laevis subgenomes, named L and S, at higher than average rates (87.1% vs 60.2%). Among the 28 singletons, 82.1% were deleted from chromosomes of the S subgenome, a rate similar to the genome-wide average (82.1% vs 74.6%). Interestingly, nkx2-1, nkx2-8, and pax9, which reside consecutively in a postulated functional gene cluster, were deleted from the S chromosome, suggesting cluster-level gene regulation. Transcriptome correlation analysis demonstrated that TF homeolog pairs tend to have more conservative developmental expression profiles than most other types of genes. In some cases, however, either of the homeologs may show strongly different spatio-temporal expression patterns, suggesting neofunctionalization, subfunctionalization, or nonfunctionalization after allotetraploidization. Analyses of otx1 suggests that homeologs with much lower expression levels have undergone greater amino acid sequence diversification. Our comprehensive study implies that TF homeologs are highly conservative after allotetraploidization, possibly because the DNA sequences that they bind were also duplicated, but in some cases, they differed in expression levels or became singletons due to dosage-sensitive regulation of their target genes. (Keyword) Xenopus laevis / genome / chromosome / transcription factor / allotetraploid (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ydbio.2016.09.017 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27810169 ● Search Scopus @ Elsevier (PMID): 27810169 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2016.09.017 (DOI: 10.1016/j.ydbio.2016.09.017, PubMed: 27810169)
5.	Adam M. Session, Yoshinobu Uno, Taejoon Kwon, Jarrod A. Chapman, Atsushi Toyoda, Shuji Takahashi, Akimasa Fukui, Akira Hikosaka, Atsushi Suzuki, Mariko Kondo, Simon Heeringen J. van, Ian Quigley, Sven Heinz, Hajime Ogino, Haruki Ochi, Uffe Hellsten, Jessica B. Lyons, Oleg Simakov, Nicholas Putnam, Jonathan Stites, Yoko Kuroki, Toshiaki Tanaka, Tatsuo Michiue, Minoru Watanabe, Ozren Bogdanovic, Ryan Lister, Georgios Georgiou, Sarita S. Paranjpe, Ila Kruijsbergen van, Shengquiang Shu, Joseph Carlson, Tsutomu Kinoshita, Yuko Ohta, Shuuji Mawaribuchi, Jerry Jenkins, Jane Grimwood, Jeremy Schmutz, Therese Mitros, Sahar V. Mozaffari, Yutaka Suzuki, Yoshikazu Haramoto, Takamasa S. Yamamoto, Chiyo Takagi, Rebecca Heald, Kelly Miller, Christian Haudenschild, Jacob Kitzman, Takuya Nakayama, Yumi Izutsu, Jacques Robert, Joshua Fortriede, Kevin Burns, Vaneet Lotay, Kamran Karimi, Yuuri Yasuoka, Darwin S. Dichmann, Martin F. Flajnik, Douglas W. Houston, Jay Shendure, Louis DuPasquier, Peter D. Vize, Aaron M. Zorn, Michihiko Ito, Edward M. Marcotte, John B. Wallingford, Yuzuru Ito, Makoto Asashima, Naoto Ueno, Yoichi Matsuda, Gert Jan C. Veenstra, Asao Fujiyama, Richard M. Harland, Masanori Taira and Daniel S. Rokhsar : Genome evolution in the allotetraploid frog Xenopus laevis., Nature, Vol.538, No.7625, 336-343, 2016. (Summary) To explore the origins and consequences of tetraploidy in the African clawed frog, we sequenced the Xenopus laevis genome and compared it to the related diploid X. tropicalis genome. We characterize the allotetraploid origin of X. laevis by partitioning its genome into two homoeologous subgenomes, marked by distinct families of 'fossil' transposable elements. On the basis of the activity of these elements and the age of hundreds of unitary pseudogenes, we estimate that the two diploid progenitor species diverged around 34 million years ago (Ma) and combined to form an allotetraploid around 17-18 Ma. More than 56% of all genes were retained in two homoeologous copies. Protein function, gene expression, and the amount of conserved flanking sequence all correlate with retention rates. The subgenomes have evolved asymmetrically, with one chromosome set more often preserving the ancestral state and the other experiencing more gene loss, deletion, rearrangement, and reduced gene expression. (Keyword) Xenopus laevis / genome / chromosome / allotetraploid (Tokushima University Institutional Repository) ● Metadata: 110155 (Link to Publication Site) ● Publication site (DOI): 10.1038/nature19840 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27762356 ● Search Scopus @ Elsevier (PMID): 27762356 ● Search Scopus @ Elsevier (DOI): 10.1038/nature19840 (Tokushima University Institutional Repository: 110155, DOI: 10.1038/nature19840, PubMed: 27762356)
6.	k Tanaka, A Kato, C Angelocci, Minoru Watanabe and Y Kato : A potential molecular pathogenesis of cardiac/laterality defects in Oculo-Facio-Cardio-Dental syndrome., Developmental Biology, Vol.387, No.1, 28-36, 2014. (Summary) Pitx2 is the last effector of the left-right (LR) cascade known to date and plays a crucial role in the patterning of LR asymmetry. In Xenopus embryos, the expression of Pitx2 gene in the left lateral plate mesoderm (LPM) is directly regulated by Xnr1 signaling, which is mediated by Smads and FoxH1. Previous studies suggest that the suppression of Pitx2 gene in the left LPM is a potential cause of cardiac/laterality defects in Oculo-Facio-Cardio-Dental (OFCD) syndrome, which is known to be caused by mutations in BCL6 co-repressor (BCOR) gene. Recently, our work has revealed that the BCL6/BCOR complex blocks Notch-dependent transcriptional activity to protect the expression of Pitx2 in the left LPM from the inhibitory activity of Notch signaling. These studies indicated that uncontrolled Notch activity in the left LPM caused by dysfunction of BCOR may result in cardiac/laterality defects of OFCD syndrome. However, this Notch-dependent inhibitory mechanism of Pitx2 gene transcription still remains unknown. Here we report that transcriptional repressor ESR1, which acts downstream of Notch signaling, inhibits the expression of Pitx2 gene by binding to a left side-specific enhancer (ASE) region in Pitx2 gene and recruiting histone deacetylase 1 (HDAC1) to this region. Once HDAC1 is tethered, histone acetyltransferase p300 is no longer recruited to the Xnr1-dependent transcriptional complex on the ASE region, leading to the suppression of Pitx2 gene in the left LPM. The study presented here uncovers the regulatory mechanism of Pitx2 gene transcription which may contribute to an understanding of pathogenesis of OFCD syndrome. (Keyword) Animals / Base Sequence / Binding Sites / Body Patterning / Cataract / DNA-Binding Proteins / Enhancer Elements, Genetic / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Heart / Heart Defects, Congenital / Heart Septal Defects / Heterotaxy Syndrome / Histone Deacetylase 1 / Homeodomain Proteins / Mesoderm / Microphthalmos / Molecular Sequence Data / Protein Binding / Receptors, Notch / Repressor Proteins / Signal Transduction / Smad Proteins / Transcription Factors / Transcriptional Activation / Xenopus Proteins / Xenopus laevis / p300-CBP Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ydbio.2014.01.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24440151 ● Summary page in Scopus @ Elsevier: 2-s2.0-84893724977 (DOI: 10.1016/j.ydbio.2014.01.003, PubMed: 24440151, Elsevier: Scopus)
7.	Makoto Yanagisawa, Keiko Kashiwagi, Hideki Hanada, Tadashi Shinkai, Satoshi Yoshitome, Hideo Kubo, Masao Sakai, Hirotada Fujii, Masamichi Yamashita, Akihiko Kashiwagi, Minoru Watanabe and Nobuaki Furuno : Hypergravity Specifically Affects Anterior Head Formation in Early Xenopus Embryos, Biological Sciences in Space, Vol.26, 47-52, 2013. (Link to Publication Site) ● Publication site (DOI): 10.2187/bss.26.47 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.2187/bss.26.47 (DOI: 10.2187/bss.26.47)
8.	Makoto Yanagisawa, Keiko Kashiwagi, Hideki Hanada, Tadashi Shinkai, Satoshi Yoshitome, Hideo Kubo, Masao Sakai, Hirotada Fujii, Masamichi Yamashita, Akihiko Kashiwagi, Nobuaki Furuno and Minoru Watanabe : Analysis of Head-Defects Caused by Hypergravity in Early Xenopus Embryos, Biological Sciences in Space, Vol.26, 1-6, 2012. (Link to Publication Site) ● Publication site (DOI): 10.2187/bss.26.1 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.2187/bss.26.1 (DOI: 10.2187/bss.26.1)
9.	柳澤誠, 古野伸明, Minoru Watanabe, 柏木啓子, 花田秀樹, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 鈴木賢一, 山下雅道 and 柏木明彦 : Hypergravity specifically effects head formation in the Xenopus embryos raised under hypergravity condition, Space Utilization Research, Vol.28, 172-175, 2012.
10.	柳澤誠, 古野伸明, Minoru Watanabe, 柏木啓子, 花田秀樹, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 鈴木賢一, 山下雅道 and 柏木昭彦 : 過重力によって生じたアフリカツメガエル初期胚の頭部形成異常の解析 II, Space Utilization Research, Vol.27, 198-200, 2011. (Keyword) Xenopus / hypergravity / head formation / gene expression
11.	Takanori Satoh, Tetsuo Sayama, Keiji Tani, Yukari Nakahara, Junko Fujimoto and Minoru Watanabe : A Report from Experience-based Study Programs of Life Science for High School Students by University-High School Cooperation: Examples of SPP and SSH Programs., Journal of University Education Research, No.7, 173-186, 2010. (Tokushima University Institutional Repository) ● Metadata: 82851 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050020697878018432 (Tokushima University Institutional Repository: 82851, CiNii: 1050020697878018432)
12.	柏木桂子, 藤原好恒, 坂尾智美, 古野伸明, 柳澤誠, 花田秀樹, 谷本能文, 山下雅道, Minoru Watanabe, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 内藤富夫, 鈴木賢一 and 柏木昭彦 : Effect of strong static magnetic fields on amphibian life cycle-morphological and milecular biological analysis of early development-, Space Utilization Research, Vol.26, 232-235, 2010.
13.	Minoru Watanabe, 柳澤誠, 古野伸明, 柏木啓子, 花田秀樹, 新海正, 吉留賢, 久保英夫, 酒井雅夫, 藤井博匡, 山下雅道 and 柏木昭彦 : 過重力下で見られるアフリカツメガエル頭部形成異常の解析, Space Utilization Research, Vol.25, 155-158, 2009.
14.	Kanako Waki, Akiko Noami, Mikiko Hashimoto, Akio Okuma, Ituka Kubo, Taku Kanai, Takanori Satoh, Minoru Watanabe and Toshio Gotoh : Novel S-S loops in the globin chains from the giant hemoglobin of the polychaete Perinereis aibuhitensis., Natural Science Research, Vol.22, 105-125, 2008. (Tokushima University Institutional Repository) ● Metadata: 62134 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050020697878137472 (Tokushima University Institutional Repository: 62134, CiNii: 1050020697878137472)
15.	H Ueno, N Nakajo, Minoru Watanabe, M Isoda and N Sagata : FoxM1-driven cell division is required for neuronal differentiation in early Xenopus embryos., Development, Vol.135, No.11, 2023-2030, 2008. (Summary) In vertebrate embryogenesis, neural induction is the earliest step through which the fate of embryonic ectoderm to neuroectoderm becomes determined. Cells in the neuroectoderm or neural precursors actively proliferate before they exit from the cell cycle and differentiate into neural cells. However, little is known about the relationship between cell division and neural differentiation, although, in Xenopus, cell division after the onset of gastrulation has been suggested to be nonessential for neural differentiation. Here, we show that the Forkhead transcription factor FoxM1 is required for both proliferation and differentiation of neuronal precursors in early Xenopus embryos. FoxM1 is expressed in the neuroectoderm and is required for cell proliferation in this region. Specifically, inhibition of BMP signaling, an important step for neural induction, induces the expression of FoxM1 and its target G2-M cell-cycle regulators, such as Cdc25B and cyclin B3, thereby promoting cell division in the neuroectoderm. Furthermore, G2-M cell-cycle progression or cell division mediated by FoxM1 or its target G2-M regulators is essential for neuronal differentiation but not for specification of the neuroectoderm. These results suggest that FoxM1 functions to link cell division and neuronal differentiation in early Xenopus embryos. (Keyword) Animals / Bone Morphogenetic Proteins / Carrier Proteins / Cell Cycle / Cell Differentiation / Cell Division / Cell Proliferation / Embryo, Nonmammalian / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Immunoblotting / In Situ Hybridization / Models, Biological / Neurons / Reverse Transcriptase Polymerase Chain Reaction / Xenopus Proteins / Xenopus laevis (Link to Publication Site) ● Publication site (DOI): 10.1242/dev.019893 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18469223 ● Search Scopus @ Elsevier (PMID): 18469223 ● Search Scopus @ Elsevier (DOI): 10.1242/dev.019893 (DOI: 10.1242/dev.019893, PubMed: 18469223)
16.	新海正, 柏木昭彦, 柏木啓子, 古野伸明, 花田秀樹, 吉留賢, Minoru Watanabe, 藤井博匡, 坂井雅夫 and 山下雅道 : 過重力によるニシツメガエル脳下垂体‐甲状腺系の絹小低下に対する甲状腺ホルモン投与の効果, Space Utilization Research, Vol.24, 242-245, 2008.
17.	古野伸明, 田澤一朗, 柏木啓子, 新海正, 吉留賢, Minoru Watanabe, 山下雅道 and 柏木昭彦 : アフリカツメガエル卵成熟過程に対する過重力の影響II, Space Utilization Research, Vol.23, 311-313, 2007.
18.	Tadashi Shinkai, Akihiko Kashiwagi, Keiko Kashiwagi, Michihiko Matsuda, Shiro Urano, Hiroya Sato, Hideo Kubo, Nobuaki Furuno, Masao Sakai, Minoru Watanabe, Satoshi Yoshitome, Hirotada Fujii and Masamichi Yamashita : Effects of Hypergravity on Pituitary-Target Organs in the Frog, Xenopus laevis, Biological Sciences in Space, Vol.20, No.2, 40-43, 2006. (Keyword) Hypergravity / Pituitary / Thyroid / Long-term breeding / Xenopus laevis (Link to Publication Site) ● Publication site (DOI): 10.2187/bss.20.40 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679408433792 ● Search Scopus @ Elsevier (DOI): 10.2187/bss.20.40 (DOI: 10.2187/bss.20.40, CiNii: 1390282679408433792)
19.	Tadashi Shinkai, Akihiko Kashiwagi, Keiko Kashiwagi, Nobuaki Furuno, Shiro Urano, Hiroya Sato, Hideo Kubo, Masao Sakai, Minoru Watanabe, Satoshi Yoshitome, Hirotada Fujii and Masamichi Yamashita : Effects on hypergravity on pituitary-target organ axes in the frog, Xenopus laevis, Space Utilization Research, Vol.22, 231-234, 2006.
20.	Nobiaki Furuno, Ichiro Tazawa, Keiko Kashiwagi, Satomi Kawakami, Satoshi Yoshitome, Minoru Watanabe, Masamichi Yamashita and Akihiko Kashiwagi : Effects of the hypergravity on oocyte maturation of Xenopus laevis, Space Utilization Research, Vol.22, 228-230, 2006.
21.	Akihiko Kashiwagi, Nobuaki Furuno, Keiko Kashiwagi, Hideki Hanada, Satomi Kawakami, Minoru Watanabe and Masamichi Yamashita : Effects of gravity on the amphibian life cycle - morphological and molecular biological analyses of early development, morphogenesis and metamorphosis, Space Utilization Research, Vol.21, 270-273, 2005.
22.	Minoru Watanabe, L Martha Rebbert, Massimiliano Andreazzoli, Nobuhiro Takahashi, Reiko Toyama, Steven Zimmerman, Malcolm Whitman and B Igor Dawid : Regulation of the Lim-1 gene is mediated through conserved FAST-1/FoxH1 sites in the first intron, Developmental Dynamics, Vol.225, No.4, 448-456, 2002. (Summary) The Lim-1 gene encodes a LIM-homeodomain transcription factor that is highly conserved among vertebrates and is required for successful gastrulation and head formation. The expression of this gene in the mesoderm of the gastrula is known to require an activin/nodal signal. Earlier studies have shown that the Xenopus Lim-1 (Xlim-1) gene contains an activin response element (ARE) in its first intron, which cooperates with an activin-unresponsive upstream promoter in the regulation of the gene. Here, we show that the Xlim-1 ARE contains a cluster of FAST-1/FoxH1 and Smad4 recognition sites; such sites have been shown to mediate activin/nodal responses in other genes. By using reporter constructs with mutated FAST-1/FoxH1 sites and FAST-1/FoxH1 protein chimeras, we show that the regulation of Xlim-1 by activin depends on FAST-1/FoxH1 function. Comparative studies on the zebrafish lim1 gene indicate the presence of FoxH1 sites in the first intron of this gene and provide evidence for the requirement for FoxH1 function in its regulation. These results illuminate the conserved nature of the transcriptional regulation of the Lim-1 gene in different vertebrate animals. (Keyword) Activins / Animals / Base Sequence / Binding Sites / Conserved Sequence / DNA-Binding Proteins / Evolution, Molecular / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Genes, Reporter / Homeodomain Proteins / Introns / LIM-Homeodomain Proteins / Models, Biological / Models, Genetic / Molecular Sequence Data / Nodal Protein / Protein Binding / RNA, Messenger / Sequence Homology, Nucleic Acid / Signal Transduction / Smad4 Protein / Trans-Activators / Transcription Factors / Transforming Growth Factor beta / Xenopus / Xenopus Proteins / Zebrafish / Zebrafish Proteins (Link to Publication Site) ● Publication site (DOI): 10.1002/dvdy.10176 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12454922 ● Search Scopus @ Elsevier (PMID): 12454922 ● Search Scopus @ Elsevier (DOI): 10.1002/dvdy.10176 (DOI: 10.1002/dvdy.10176, PubMed: 12454922)
23.	Hidetaka Shiratori, Rui Sakuma, Minoru Watanabe, Hiromi Hashiguchi, Kyoko Mochida, Yasuo Sakai, Jinsuke Nishino, Yukio Saijoh, Malcolm Whitman and Hiroshi Hamada : Two step regulation of left-right asymmetric Pitx2 expression: Initiation by Nodal signaling and maintenance by Nkx2, Molecular Cell, Vol.7, No.1, 137-149, 2001. (Summary) Pitx2 is left--right (L--R) asymmetrically expressed initially in the lateral plate and later in primordial visceral organs. The transcriptional regulatory mechanisms that underlie L--R asymmetric expression of Pitx2 were investigated. Mouse Pitx2 has a left side-specific enhancer (ASE) that mediates both the initiation and maintenance of L--R asymmetric expression. This element contains three binding sites for the transcription factor FAST. The FAST binding sites function as Nodal-responsive elements and are sufficient for the initiation but not for the maintenance of asymmetric expression. The maintenance requires an Nkx2-5 binding site also present within the ASE. These results suggest that the left-sided expression of Pitx2 is directly initiated by Nodal signaling and is subsequently maintained by Nkx2. Such two-step control may represent a general mechanism for gene regulation during development. (Keyword) Animals / Base Sequence / Binding Sites / Body Patterning / DNA-Binding Proteins / Enhancer Elements, Genetic / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Homeodomain Proteins / Lac Operon / Left-Right Determination Factors / Mice / Molecular Sequence Data / Nodal Protein / Nuclear Proteins / Paired Box Transcription Factors / Signal Transduction / Transcription Factors / Transforming Growth Factor beta / Transgenes / Xenopus / Xenopus Proteins (Link to Publication Site) ● Publication site (DOI): 10.1016/S1097-2765(01)00162-9 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11172719 ● CiNii @ National Institute of Informatics (CRID): 1363951795033869056 ● Summary page in Scopus @ Elsevier: 2-s2.0-17744364498 (DOI: 10.1016/S1097-2765(01)00162-9, PubMed: 11172719, CiNii: 1363951795033869056, Elsevier: Scopus)
24.	Shin-Ichi Osada, Yukio Saijoh, Amanda Frisch, Chang-Yeol Yeo, Hitoshi Adachi, Minoru Watanabe, Malcolm Whitman, Hiroshi Hamada and VE Christopher Wright : Activin/Nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1, Development, Vol.127, No.11, 2503-2514, 2000. (Summary) Vertebrate Nodal-related factors play central roles in mesendoderm induction and left-right axis specification, but the mechanisms regulating their expression are largely unknown. We identify an element in Xnr1 intron 1 that is activated by activin and Vg1, autoactivated by Xnrs, and suppressed by ventral inducers like BMP4. Intron 1 contains three FAST binding sites on which FAST/Smad transcriptional complexes can assemble; these sites are differentially involved in intron 1-mediated reporter gene expression. Interference with FAST function abolishes intron 1 activity, and transcriptional activation of Xnrs by activin in embryonic tissue explant assays, identifying FAST as an essential mediator of Xnr autoregulation and/or 'signal relay' from activin-like molecules. Furthermore, the mapping of endogenous activators of the Xnr1 intronic enhancer within Xenopus embryos agrees well with the pattern of Xnr1 transcription during embryogenesis. In transgenic mice, Xnr1 intron 1 mimics a similarly located enhancer in the mouse nodal gene, and directs FAST site-dependent expression in the primitive streak during gastrulation, and unilateral expression during early somitogenesis. The FAST cassette is similar in an ascidian nodal-related gene, suggesting an ancient origin for this regulatory module. Thus, an evolutionarily conserved intronic enhancer in Xnr1 is involved in both mesendoderm induction and asymmetric expression during left-right axis formation. (Keyword) Activins / Animals / Binding Sites / DNA-Binding Proteins / Enhancer Elements, Genetic / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Inhibins / Introns / Mice / Mice, Inbred C3H / Mice, Inbred C57BL / Mice, Transgenic / Nodal Protein / Response Elements / Transcription Factors / Transforming Growth Factor beta / Transgenes / Xenopus Proteins / Xenopus laevis (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10804190 ● Summary page in Scopus @ Elsevier: 2-s2.0-0034044575 (PubMed: 10804190, Elsevier: Scopus)
25.	Yukio Saijoh, Hitoshi Adachi, Rui Sakuma, Chang-Yeol Yeo, Kenta Yashiro, Minoru Watanabe, Hiromi Hashiguchi, Kyoko Mochida, Sachiko Ohishi, Masahiro Kawabata, Kohei Miyazono, Malcolm Whitman and Hiroshi Hamada : Left-right asymmetric expression of lefty2 and nodal is induced by a signaling pathway that includes the transcription factor FAST2, Molecular Cell, Vol.5, No.1, 35-47, 2000. (Summary) The left-right (L-R) asymmetric expression of lefty2 and nodal is controlled by a left side-specific enhancer (ASE). The transcription factor FAST2, which can mediate signaling by TGF beta and activin, has now been identified as a protein that binds to a conserved sequence in ASE. These FAST2 binding sites were both essential and sufficient for L-R asymmetric gene expression. The Fast2 gene is bilaterally expressed when nodal and lefty2 are expressed on the left side. TGF beta and activin can activate the ASE activity in a FAST2-dependent manner, while Nodal can do so in the presence of an EGF-CFC protein. These results suggest that the asymmetric expression of lefty2 and nodal is induced by a left side-specific TGF beta-related factor, which is most likely Nodal itself. (Keyword) Animals / Base Sequence / Cloning, Molecular / Conserved Sequence / DNA-Binding Proteins / Embryo, Mammalian / Embryo, Nonmammalian / Enhancer Elements, Genetic / Feedback / Forkhead Transcription Factors / Gene Expression Regulation / Left-Right Determination Factors / Mice / Mice, Transgenic / Nodal Protein / Recombinant Fusion Proteins / Recombinant Proteins / Signal Transduction / Transcription Factors / Transcription, Genetic / Transforming Growth Factor beta / Xenopus laevis (Link to Publication Site) ● Publication site (DOI): 10.1016/S1097-2765(00)80401-3 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10678167 ● Summary page in Scopus @ Elsevier: 2-s2.0-0033969935 (DOI: 10.1016/S1097-2765(00)80401-3, PubMed: 10678167, Elsevier: Scopus)
26.	Minoru Watanabe and Malcolm Whitman : The role of transcription factors involved in TGFß superfamily signaling during development, Cellular and Molecular Biology, Vol.45, No.5, 537-543, 1999.
27.	Minoru Watanabe and Malcolm Whitman : FAST-1 is a key maternal effector of mesodermal inducers in the early Xenopus embryo, Development, Vol.126, 5621-5634, 1999.
28.	Gretchen Poortinga, Minoru Watanabe and M. Susan Parkhurst : Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and Hairy-mediated transcriptional repression, The EMBO Journal, Vol.17, 2067-2078, 1998.
29.	Xin Chen, Ellen Weisberg, Valerie Fridmacher, Minoru Watanabe, Grace Naco and Malcolm Whitman : Smad4 and FAST-1 in the assembly of activin-responsive factor, Nature, Vol.389, No.6646, 85-89, 1997. (Summary) Members of the TGF-beta superfamily of signalling molecules work by activating transmembrane receptors with phosphorylating activity (serine-threonine kinase receptors); these in turn phosphorylate and activate SMADs, a class of signal transducers. Activins are growth factors that act primarily through Smad2, possibly in partnership with Smad4, which forms heteromeric complexes with different ligand-specific SMADs after activation. In frog embryos, Smad2 participates in an activin-responsive factor (ARF), which then binds to a promoter element of the Mix.2 gene. The principal DNA-binding component of ARF is FAST-1, a transcription factor with a novel winged-helix structure. We now report that Smad4 is present in ARF, and that FAST-1, Smad4 and Smad2 co-immunoprecipitate in a ligand-regulated fashion. We have mapped the site of interaction between FAST-1 and Smad2/Smad4 to a novel carboxy-terminal domain of FAST-1, and find that overexpression of this domain specifically inhibits activin signalling. In a yeast two-hybrid assay, the FAST-1 carboxy terminus interacts with Smad2 but not Smad4. Deletion mutants of the FAST-1 carboxy terminus that still participate in ligand-regulated Smad2 binding no longer associated with Smad4 or ARF. These results indicate that Smad4 stabilizes a ligand-stimulated Smad2-FAST-1 complex as an active DNA-binding factor. (Keyword) Activins / Animals / Binding Sites / DNA / DNA-Binding Proteins / Electrophoresis / Embryo, Nonmammalian / Epitopes / Forkhead Transcription Factors / Humans / Inhibins / Macromolecular Substances / Mice / Molecular Sequence Data / Precipitin Tests / Signal Transduction / Smad2 Protein / Smad4 Protein / Trans-Activators / Transcription Factors / Xenopus / Xenopus Proteins (Link to Publication Site) ● Publication site (DOI): 10.1038/38008 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9288972 ● Search Scopus @ Elsevier (PMID): 9288972 ● Search Scopus @ Elsevier (DOI): 10.1038/38008 (DOI: 10.1038/38008, PubMed: 9288972)
30.	Naoyuki Hayashi, Hiroaki Seino, Kenji Irie, Minoru Watanabe, KL Clark, Kunihiro Matsumoto and Takeharu Nishimoto : General interaction of DED1 encoding a putative ATP-dependent RNA helicase with SRM1 encoding a mammalian RCC1 homologue in Saccharomyces cerevisiae, Molecular and General Genetics, Vol.253, 149-156, 1996.
31.	Minoru Watanabe, R. Andrew Zinn, C. David Page and Takeharu Nishimoto : Functional equivalence of human X- and Y-encoded isoforms of ribosomal protein S4 consistent with a role in Turner syndrome, Nature Genetics, Vol.4, No.3, 268-271, 1993. (Summary) Several genes are found on both the human X and Y chromosomes in regions that do not recombine during male meiosis. In each case, nucleotide sequence analysis suggests that these X-Y gene pairs encode similar but nonidentical proteins. Here we show that the human Y- and X-encoded ribosomal proteins, RPS4Y and RPS4X, are interchangeable and provide an essential function: either protein rescued a mutant hamster cell line that was otherwise incapable of growth at modestly elevated temperatures. These findings are consistent with the hypothesis that RPS4 deficiency has a role in Turner syndrome, a complex human phenotype associated with monosomy X. (Keyword) Amino Acid Sequence / Animals / Base Sequence / Cricetinae / DNA / Female / Genetic Complementation Test / Humans / Hybrid Cells / Male / Molecular Sequence Data / Mutation / Phenotype / Ribosomal Proteins / Temperature / Turner Syndrome / X Chromosome / Y Chromosome (Link to Publication Site) ● Publication site (DOI): 10.1038/ng0793-268 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8358435 ● Search Scopus @ Elsevier (PMID): 8358435 ● Search Scopus @ Elsevier (DOI): 10.1038/ng0793-268 (DOI: 10.1038/ng0793-268, PubMed: 8358435)
32.	Minoru Watanabe, Toshiro Hayashida, Takeharu Nishimoto and Hideki Kobayashi : Nucleotide sequence of Xenopus homeobox gene, En-1, Nucleic Acids Research, Vol.21, 2513, 1993.
33.	Minoru Watanabe, Nobuaki Furuno, Mark Gobel, Mitiko Go, Kumi Miyauchi, Takeshi Sekiguchi, Claudio Basilico and Takeharu Nishimoto : Molecular cloning of the human gene, CCG2, that complements the BHK-derived temperature-sensitive cell cycle mutant tsBN63: identification of CCG2 with the human X chromosomal SCAR/RPS4 gene, Journal of Cell Science, Vol.100, 35-43, 1991.

Academic Paper (Unrefereed Paper):

1.	藤本順子, 徳島県立城北高等学校サイエンス部, 小川誠, Minoru Watanabe and 米澤義彦 : Characteristics of hybrids between Taraxacum japonicum and T. officinale (Asteraceae, Asterales) growing in urban parks of Tokushima city, western Japan, Bull. Tokushima Pref. Mus., No.34, 65-76, 2024.
2.	Minoru Watanabe, Tadashi Yamashiro, Takanori Satoh, Makoto Ohashi, Hideaki Maseda, Shyouzo Tsuzuki and Takao Hanabusa : University and High school cooperation project-A report from experience-based study program of high school students in the laboratory of the university-, Journal of University Education Research, No.6, 85-101, 2009. (Tokushima University Institutional Repository) ● Metadata: 82694 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050020697878028800 (Tokushima University Institutional Repository: 82694, CiNii: 1050020697878028800)
3.	Makoto Ohashi, Minoru Watanabe, Takanori Satoh and Hiroyoshi Ishii : A practical training in enveronmental education for traversal thinking of a nature and historical heritage of headstream of Yoshino river;a educational significance of environmental education for symbiosis, Journal of University Education Research, No.6, 115-119, 2009. (Tokushima University Institutional Repository) ● Metadata: 82697 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050583647831449472 (Tokushima University Institutional Repository: 82697, CiNii: 1050583647831449472)
4.	山中瑞江, 脇加奈子, 尾崎絵里香, 金井拓, 金井悠一郎, 中辻嵩, 谷川和代, 橋本美城子, 森由美子, 倉林陽子, 岸本慎哉, 水本浩太, Minoru Watanabe, Masaya Satoh, Kazuhiro W. Makabe, Makoto Ohashi and Toshio Gotoh : Comparative study on the primary structure of globin chains of the extracellular giant hemoglobin from the polychaet Perinereis aibuhitensis and Tylonrrhynchus heterochaetus, Natural Science Research, Faculty of Integrated Arts and Sciences, The University of Tokushima, Vol.19, 63-92, 2005. (Keyword) giant hemoglobin / annelid / polychaete / phylogenetic tree / supramolecule (Tokushima University Institutional Repository) ● Metadata: 117481 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520290882530406912 (Tokushima University Institutional Repository: 117481, CiNii: 1520290882530406912)

Academic Letter:

1.	Takanori Satoh, 石田啓祐, 小山晋之, 久田旭彦, Minoru Watanabe, 秋山治彦 and 三好徳和 : 高校生向け課題研究研修会によるアクティブ・ラーニング型高大連携とFDへの展開, Education Conference, the University of Tokushima, Abstracts(2016), 28-29, 2016.
2.	Atsushi Tsuchiya, Hiroaki Mikasa, Minoru Watanabe, Masayoshi Shinhara and 古澤修一 : アクティブ・ラーニングを取り入れた授業実践, 平成28年度徳島大学「大学教育再生加速プログラム」シンポジウムアクティブ・ラーニング&反転授業発表抄録集, 4-5, 2016.
3.	Minoru Watanabe, Takanori Satoh and Ohashi M : A report from experience-based study program of high school students in the laboratory of the university (4), Education Conference, the University of Tokushima, Abstracts(2012), 54-55, 2012.
4.	Minoru Watanabe, Takanori Satoh, Yamashiro T and Ohashi M : A report from experience-based study program of high school students in the laboratory of the university (3), Education Conference, the University of Tokushima, Abstracts(2011), 66-67, 2012.
5.	Minoru Watanabe, 山城考, Takanori Satoh, 大橋眞, 間世田英明, 續木章三 and 英崇夫 : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告, 平成20年度全学FD徳島大学教育カンファレンス発表抄録集, 8-9, 2009.

Review, Commentary:

1.	Minoru Watanabe : FAST-1: TGFßシグナルによる中胚葉誘導に必須の転写因子, 細胞工学, Vol.19, 280-282, 2000.

Proceeding of International Conference:

1.	Keiji Minagawa, Minoru Watanabe, Takahito Saito and Hiroyuki Ukida : Learning through Teaching Programs of Science and Technology for University Students, 8th Asian Conference on Engineering Education (ACEE2019), Innovation in Education for Global Business, 175-180, Kota Kinabalu, Jun. 2019.
2.	Keiji Minagawa, Minoru Watanabe and Takahito Saito : Design of General/Basic Education Courses Utilizing Learning through Teaching at School Visit Programs, 7th Asian Conference on Engineering Education (ACEE2018), Niigata, Jun. 2018.
3.	Ryutaroh Tanaka, Miyu Yoshida, Yoshitome Satoshi, Furuno Nobuaki, Nakajo Nobushige and Minoru Watanabe : Developmental roles for Wee1B, a negative regulator for the cell cycle, in Xenopus laevis, International Symposium at Hiroshima University. Amphibian development, regeneration, evolution and beyond., 26, Mar. 2018. (Keyword) Xenopus laevis / genome editing / cell cycle
4.	Minoru Watanabe : Application of CRISPR/Cas system to Xenopus laevis, International Symposium Frontiers in Amphibian Biology: Endangered Specices Consevation and Genome Editing, 59-60, Mar. 2014. (Keyword) Xenopus laevis / genome editing
5.	Mari Yamada, Takeshi Nishiyama, Yuta Tagami and Minoru Watanabe : Suppression of gene function in Xenopus laevis, International Symposium on RNAi and Genome Editing Methods, Mar. 2014. (Keyword) Xenopus laevis / genome editing / RNAi
6.	Kanako Nakada, Noriyuki Kinoshita and Minoru Watanabe : A novel zinc finger transcription factor is required for the mesoderm induction during early Xenopus development, 11th International Xenopus Conference, Abstruct, 81, Kazusa (Chiba, Japan), Sep. 2006.
7.	Minoru Watanabe : Functional analysis of a transcription factor XSPR2b during early Xenopus development, 2nd International Symposium and annual meeting of Dynamics of Developmental Systems, Abstruct, 31, Chiba (Japan), Nov. 2005.
8.	Minoru Watanabe, Junji Mirikuma, Atsushi Kitayama and Naoto Ueno : XSPR-2b, a novel Nodal/FAST-1 target gene, is involved in the early mesodermal gene expression during Xenopus development, 10th International Xenopus Conference, Abstruct, 129, Woods Hole (Massachusetts, USA), Sep. 2004.
9.	Minoru Watanabe and Malcolm Whitman : The role of the FAST-1 gene: identification as a component of mesodermal inducing signaling in the Xenopus early embryo, 7th International Xenopus conference, Abstruct, 103, Chia Laguna (Sardegna, Italy), Sep. 1998.
10.	Minoru Watanabe and Malcolm Whitman : Role of FAST-1 in early Xenopus patterning, Molecular Genetics of Development, Abstruct, 8, Airlie (Virginia, USA), May 1998.
11.	Minoru Watanabe and Malcolm Whitman : Function of the FAST-1 gene during Xenopus early development, The Society for Developmental Biology, Northeast Regional Meeting, Abstruct, 9, Woods Hole (MA, USA), Apr. 1998.
12.	Minoru Watanabe and M Susan Parkhurst : Identification of genes that interact with hairy, 36th Annual Drosophila Reserach Conference, Abstruct, 282, Atlanta (Georgia, USA), Apr. 1995.

Proceeding of Domestic Conference:

1.	Minoru Watanabe, Yuta Tagami, Takeshi Nishiyama and Michiko Omote : Application of the RNA interference technique to Xenopus embryos: Specific reduction of the β-catenin gene products by short double-stranded RNA produced by recombinant human Dicer, 第56回日本発生生物学会年会, Jul. 2023.
2.	Takuma Hayashi, Ryutaroh Tanaka, Miyu Yoshida, 中條信成, 吉留賢, 西嶋達郎, 中島圭介, 古野伸明 and Minoru Watanabe : Functional analysis of cell cycle regulators MYT1 and WEE1B in early Xenopus embryos, 第45回日本分子生物学会年会, Dec. 2022.
3.	Taisei Shiraki, Takuma Hayashi, Johtaroh Ozue and Minoru Watanabe : The Wilmus' tumor suppressor gene is required for normal pronehros development of Xenopus laevis, 第45回日本分子生物学会年会, Nov. 2022.
4.	Minoru Watanabe : 授業の課題レポートの評価方法について, 第69回中国・四国地区大学教育研究会, Jun. 2022.
5.	Taisei Shiraki, Takuma Hayashi, Johtaroh Ozue and Minoru Watanabe : Proper amount and activity of the Wilms' tumor suppressor gene, WT1, is required for normal pronephros development of Xenopus embryos, 55th Annual Meeting of the Japanese Society for Developmental Biology, Jun. 2022.
6.	Minoru Watanabe : アフリカツメガエル初期胚における RNA interference(RNAi; RNA 干渉)法による遺伝子機能の抑制, 徳島生物学会会報, Vol.74, No.1, 15, Jan. 2022.
7.	Takuma Hayashi, Ryutaroh Tanaka, Miyu Yoshida and Minoru Watanabe : アフリカツメガエル細胞周期制御因子のゲノム編集法による機能解析, 徳島生物学会会報, Vol.74, No.1, 14, Jan. 2022.
8.	Takuma Hayashi, Ryutaroh Tanaka, Miyu Yoshida and Minoru Watanabe : CRISPR/Cas9 法によるアフリカツメガエル細胞周期制御因子 Myt1 および Wee1B の機能解析, 徳島生物学会会報, 12, Feb. 2021.
9.	Minoru Watanabe : 生命科学の基礎研究におけるゲノム編集技術, 徳島生物学会会報, 11, Feb. 2021.
10.	Minoru Watanabe : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告, 第16回大学教育カンファレンスin徳島発表抄録集, 58-59, Jan. 2021.
11.	Minoru Watanabe : 日本の大学で働く(日本の大学の現実), 第42回日本分子生物学会年会フォーラム, Dec. 2019.
12.	Ryutaroh Tanaka, Miyu Yoshida, 吉留賢, 古野伸明, 中條信成 and Minoru Watanabe : CRISPR/Cas9法によるアフリカツメガエルwee1b遺伝子のノックアウトと表現型の解析, 第12回日本ツメガエル研究集会・第4回次世代両生類研究会合同シンポジウム, Sep. 2018.
13.	Minoru Watanabe, Ryutaroh Tanaka, Miyu Yoshida, Satoshi Yoshitome, Nobuaki Furuno and Nobushige Nakajo : Gene knockout and phenotypic analyses of Xenopus laevis wee1B gene, a negative regulator of cell cycle, by CRISPER/Cas9 method, Joint Annual Meeting of JSCB 51st and JSCB 70th, Jun. 2018. (Keyword) Xenopus laevis / genome editing / cell cycle
14.	Minoru Watanabe, 宇野好宣, 福井彰雅, 松田洋一 and 平良眞則 : アフリカツメガエルのサブゲノム染色体Sにおける大規模逆位の構造, 2017年度生命科学系学会合同年次大会, Dec. 2017. (Keyword) Xenopus laevis / genome / allotetraploid / inversion
15.	Minoru Watanabe, 宇野好宣, 福井彰雅, 松田洋一 and 平良眞則 : アフリカツメガエル同祖染色体間の逆位構造の解析, 第11回ツメガエル研究集会要旨集, 21, Sep. 2017. (Keyword) Xenopus laevis / genome / chromosome / inversion / homeologous chromosome
16.	Takanori Satoh, 石田啓祐, 小山晋之, 久田旭彦, Minoru Watanabe, 秋山治彦 and 三好徳和 : 高校生向け課題研究研修会によるアクティブ・ラーニング型高大連携とFDへの展開, Education Conference, the University of Tokushima, Abstracts(2016), 28-29, Dec. 2016.
17.	Minoru Watanabe : 異質四倍体生物アフリカツメガエルに対するゲノム編集, 第3回日本生物工学会西日本支部講演会講演要旨集, 17, Dec. 2016. (Keyword) Xenopus laevis / genome editing / genome
18.	三浦真紀, 岡本悠輝, 光森瞳子 and Minoru Watanabe : Kruppel-like factor 6(KLF6)によるアフリカツメガエルの胚葉形成, 日本動物学会中四国支部会報, Vol.68, 4, May 2016. (Keyword) Xenopus laevis / KLF6 / 胚葉形成
19.	岡本悠輝, 光森瞳子 and Minoru Watanabe : Kruppel-like factor 6(KLF6)によるアフリカツメガエルの胚葉形成, 第9回日本ツメガエル研究集会要旨集, 20, Sep. 2015.
20.	山田真里 and Minoru Watanabe : CRISPR/Cas9 法によるアフリカツメガエル初期胚のゲノム編集, 日本動物学会中国四国支部会報第67号, 13, May 2015.
21.	Minoru Watanabe, Tadashi Yamashiro and 小川誠 : タンポポを題材とした遺伝子解析の実験, 日本生物教育学会第98回研究発表予稿集, 30, Jan. 2015.
22.	Mari Yamada and Minoru Watanabe : Taegeted mutagenesis of Xenopus laevis gene by CRISPR/Cas system, 第47回日本発生生物学会年会, 76, May 2014. (Keyword) Xenopus laevis / genome editing
23.	山田真里 and Minoru Watanabe : 人工ヌクレアーゼのアフリカツメガエル初期胚への応用, 日本動物学会中国四国支部会報第66号, 15, May 2014.
24.	北村友哉, Minoru Watanabe, 吉留賢 and 古野伸明 : ネッタイツメガエルMyt-1遺伝子のクローニングと初期発生における機能解析, 日本動物学会中国四国支部会報第66号, 14, May 2014.
25.	Minoru Watanabe, Takanori Satoh and Ohashi M : A report from experience-based study program of high school students in the laboratory of the university (4), Education Conference, the University of Tokushima, Abstracts(2012), Dec. 2012.
26.	前高明典, 西山剛司 and Minoru Watanabe : アフリカツメガエル初期胚におけるβ-catenin遺伝子の下流因子の解析, 第128回徳島生物学会会報, 13, Dec. 2012.
27.	Minoru Watanabe : ツメガエルを用いた高大連携事業, 第6回XCIJ首都圏支部会(XCIJ-MA)兼第6回日本ツメガエル研究集会, Jul. 2012.
28.	Minoru Watanabe, Takanori Satoh, Yamashiro T and Ohashi M : A report from experience-based study program of high school students in the laboratory of the university (3), Education Conference, the University of Tokushima, Abstracts(2011), Jan. 2012.
29.	柳澤誠, 古野伸明, Minoru Watanabe, 柏木啓子, 花田秀樹, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 鈴木賢一, 山下雅道 and 柏木明彦 : 過重力はアフリカツメガエルの頭部形成(特に眼と脳)に特異的に影響を与える, 第28回宇宙利用シンポジウム, Jan. 2012.
30.	西山剛司, 麻植汐那, 水本菜摘, 山根真由香 and Minoru Watanabe : KN3クラスホメオボックス遺伝子のアフリカツメガエル初期発生における機能解析, 第5回日本ツメガエル研究集会要旨集, 20, Oct. 2011.
31.	Minoru Watanabe and Takeshi Nishiyama : Involvement of the Nk3 class homeobox gene in the regulation of mesoderm gene expression during eraly Xenopus development, 第44回日本発生生物学会年会, May 2011.
32.	柳澤誠, 古野伸明, Minoru Watanabe, 柏木啓子, 花田秀樹, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 鈴木賢一, 山下雅道 and 柏木昭彦 : 過重力によって生じたアフリカツメガエル初期胚の頭部形成異常の解析II, 第27回宇宙利用シンポジウム, Jan. 2011.
33.	西山剛司, 有家翔子, 前高明典 and Minoru Watanabe : cDNA microarray analysis of Xenopus laevis beta-catenin knock-down embryos by RNAi, 第33回日本分子生物学会年会プログラム, 218, Dec. 2010.
34.	Minoru Watanabe, Yamashiro T, Takanori Satoh and Ohashi M : A report from experience-based study program of high school students in the laboratory of the university (2), Education Conference, the University of Tokushima, Abstracts(2009), 28-29, Mar. 2010.
35.	柏木啓子, 藤原好恒, 板尾智美, 古野伸明, 柳澤誠, 花田秀樹, 谷本能文, 山下雅道, Minoru Watanabe, 新海正, 吉留賢, 久保英夫, 坂井雅夫, 藤井博匡, 内藤富夫, 鈴木賢一 and 柏木昭彦 : 両生類の生活環に対する強磁場の影響ー初期発生の形態学的および分子生物学的解析ー, 第26回宇宙利用シンポジウム, Jan. 2010.
36.	西山剛司 and Minoru Watanabe : Analysis of beta-catenin knock-dwon embryos of Xenopus laevis by RNAi, 第32回日本分子生物学会年会プログラム, 182, Dec. 2009.
37.	Minoru Watanabe and 西山剛司 : RNAi法によりbeta-catenin遺伝子の機能を抑制されたアフリカツメガエル初期胚の解析, 第3回日本ツメガエル研究集会要旨集, 25, Oct. 2009.
38.	Yuta Tagami, Yuki Okamoto, Takeshi Nishiyama and Minoru Watanabe : Application of RNAi technology to Xenopus beta-catenin gene, 日本発生生物学会第42回大会発表要旨集, 125, May 2009.
39.	Minoru Watanabe, 山城考, Takanori Satoh, 大橋眞, 間世田英明, 續木章三 and 英崇夫 : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告, 平成20年度全学FD徳島大学教育カンファレンス発表抄録集, 8-9, Jan. 2009.
40.	Minoru Watanabe, 山城考, Takanori Satoh, Makoto Ohashi, 間世田英明, Shyouzo Tsuzuki and Takao Hanabusa : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告, 第120回徳島生物学会会報, 17, Dec. 2008.
41.	田上雄大, 岡元悠輝, 西山剛司 and Minoru Watanabe : アフリカツメガエルbeta-catenin遺伝子に対するRNAi法の適応, 第31回日本分子生物学会講演要旨集, 803, Dec. 2008.
42.	上野裕之, 中條信成, Minoru Watanabe, 磯田道孝 and 佐方功幸 : FoxM1依存の細部尾分裂はツメガエル胚の神経分化に必須である, 第31回日本分子生物学会講演要旨集, 801, Dec. 2008.
43.	上野裕之, 中條信成, Minoru Watanabe, 磯田道孝 and 佐方功幸 : FoxM1依存の細胞分裂はツメガエル神経分化に必要である, 日本発生生物学会第41回大会発表要旨集, 285, May 2008.
44.	Hiryuki Ueno, Nobushige Nakajo, Minoru Watanabe, Michitaka Isoda and Noriyuki Sagata : FoxM1-driven cell division is required for neuronal differentiation in early Xenopus embryos, 日本発生生物学会第41回大会発表要旨集, 113, May 2008.
45.	仲田香奈子, 岡本悠輝, 田上雄大, 光森瞳子, 木下典行 and Minoru Watanabe : アフリカツメガエル初期胚におけるKLF遺伝子群の解析, 第1回日本ツメガエル研究集会要旨集, 17, Aug. 2007.
46.	村田碧 and Minoru Watanabe : アフリカツメガエルXSPR2b遺伝子の発現調節機構の研究, 平成19年度生物系三学会中国四国支部大会，会報, 2, May 2007.
47.	仲田香奈子 and Minoru Watanabe : アフリカツメガエルの中胚葉形成に重要な機能を持つ新しい転写因子の研究, 平成19年度生物系三学会中国四国支部大会，会報, 1, May 2007.
48.	上野裕之, 中條信成 and Minoru Watanabe : 細胞周期制御転写因子FoxM1はツメガエル胚神経領域の細胞分裂と神経分化に必須である, 日本分子生物学会2006フォーラム，講演要旨集, 468, Dec. 2006.
49.	Minoru Watanabe : 高校生の大学研究室への体験入学∼「ひらめき☆ときめきサイエンス」プログラム, 第116回徳島生物学会会報, 15, Dec. 2006.
50.	山尾郁美, 村田碧, 諸熊淳治, 喜多山篤, 直人上野 and Minoru Watanabe : Functional analysis and transcriptional regulation of XSPR2b gene during early Xenopus development, 日本発生生物学会第39回大会，発表要旨集, 148, Jun. 2006.
51.	山尾郁美, 村田碧, 仲田香奈子 and Minoru Watanabe : アフリカツメガエルXSPR2遺伝子の発現調節機構と機能の解析, 平成18年度生物系三学会中国四国支部大会，会報, 18, May 2006.
52.	Minoru Watanabe, 水本浩太, 山中瑞恵, Kazuhiro W. Makabe and Toshio Gotoh : アオゴカイ巨大ヘモグロビンのグロビンmRNAとその発現組織, 平成18年度生物系三学会中国四国支部大会，会報, 18, May 2006.
53.	水本浩太, 山中瑞江, Kazuhiro W. Makabe, Minoru Watanabe and Toshio Gotoh : アオゴカイ細胞外ヘモグロビンのグロビン鎖に含まれるCyc残基の位置とグロビン鎖の共通名, 第116回徳島生物学会会報, 15, Dec. 2005.
54.	中條信成, 上野裕之, Minoru Watanabe, 住友洋美, 毛利宣子 and 佐方功幸 : Cyclin E2 is essential for normal embryogenesis in Xenopus laevis, 第28回日本分子生物学会年会，講演要旨集, 701, Dec. 2005.
55.	山尾郁美, 諸熊淳治, 喜多山篤, 直人上野 and Minoru Watanabe : Functional analysis of a transcription factor XSPR2b during early Xenopus development, 第28回日本分子生物学会年会，講演要旨集, 663, Dec. 2005.
56.	Valerie Fridmacher, Minoru Watanabe, Chang-Yeol Yeo, Melissa Rubock and Malcolm Whitman : A novel member of the Rel/NFkB family binds to activin-responsive promoter elements and regulates gene expression in the Xenopus embryo, 日本発生生物学会第38回大会，発表要旨集, 230, Jun. 2005.
57.	中條信成, 住友洋美, 毛利宣子, Minoru Watanabe and 佐方功幸 : サイクリンE2は原腸胚期以降のツメガエル正常発生に必要である, 日本発生生物学会第38回大会，発表要旨集, 98, Jun. 2005.
58.	Toshio Gotoh, 尾崎絵理香, 野網明子, 金井拓, 水本浩太, Minoru Watanabe and Kazuhiro W. Makabe : アオゴカイPerinereis aibuhitensis巨大ヘモグロビンのアミノ酸配列, 平成17年度生物系三学会中国四国支部大会岡山大会，会報, 18, May 2005.
59.	Minoru Watanabe : アフリカツメガエルを用いた初期発生における形態形成機構の研究ーTGFβシグナルとその下流因子の機能解析ー, 第113回徳島生物学会会報, 7, Jun. 2004.
60.	Minoru Watanabe, 諸熊淳治, 喜多山篤, 上野直人 and 中川敏幸 : Nodal/FAST-1シグナル標的遺伝子XSPR-2bのアフリカツメガエル初期発生における機能の解析, 第26回日本分子生物学会年会，講演要旨集, 1006, Dec. 2003.
61.	Minoru Watanabe, 諸熊淳治, 喜多山篤, 上野直人 and 中川敏幸 : Nodalシグナルの下流転写因子FAST-1の標的遺伝子(アフリカツメガエル初期胚由来)の探索と機能解析, 第25回日本分子生物学会年会，講演要旨集, 892, Dec. 2002.
62.	Minoru Watanabe and Malcolm Whitman : FAST-1はアフリカツメガエル初期胚におけるアクチビン/TGFßによる中胚葉誘導に必須の因子である, 第22回日本分子生物学会年会，講演要旨集, 592, Dec. 1999.
63.	Minoru Watanabe and 西本毅治 : 温度感受性変異株tsBN63株の変異遺伝子の単離, 第14回日本分子生物学会年会，講演要旨集, Dec. 1992.
64.	Minoru Watanabe, 古野伸明, 宮内久美 and 西本毅治 : 温度感受性変異株tsBN63株を相補するヒト遺伝子のクローニング, 第13回日本分子生物学会年会，講演要旨集, Dec. 1991.
65.	Minoru Watanabe, 古野伸明, 宮内久美 and 西本毅治 : 温度感受性変異株tsBN63株を相補するヒト遺伝子のクローニング, 第13回日本分子生物学会年会，講演要旨集, 2278, Dec. 1990.
66.	林田敏朗, Minoru Watanabe, 西本毅治 and 小林英紀 : アフリカツメガエル(xenopus leavis)ホメオボックス遺伝子・Xen-1のcDNAの構造及びその産物の解析, 第13回日本分子生物学会年会，講演要旨集, 2217, Dec. 1990.
67.	Minoru Watanabe, 林田敏朗, 山名清隆 and 小林英紀 : アフリカツメガエルのengrailed遺伝子のクローニング, 第11回日本分子生物学会年会，講演要旨集, Dec. 1989.

Et cetera, Workshop:

1.	Minoru Watanabe : 対面授業と遠隔授業を取り入れた学生実習(生物学)の実践, 第14回理学系大学教育に関する研究フォーラム, Nov. 2022.
2.	Minoru Watanabe and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験学習型プログラム」実施報告(第8報), 平成29年度全学FD推進プログラム大学共育カンファレンス in 徳島発表抄録集, 58-59, Jan. 2018. (Keyword) 高大連携
3.	Minoru Watanabe and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験型学習プログラム」実施報告(第7報), 平成28年度全学FD推進プログラム大学教育カンファレンス in 徳島, 48-49, Dec. 2016. (Keyword) 高大連携 / 科学実験
4.	Takanori Satoh and Minoru Watanabe : 「高校生物の復習テスト」結果について, FD企画平成27年度大学入門講座・オリエンテーション反省会, Sep. 2015.
5.	Minoru Watanabe and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告(第6報), 平成26年度全学FD大学教育カンファレンス in 徳島発表抄録集, 48-49, Dec. 2014.
6.	Minoru Watanabe : CRISPR/Cas9法によるアフリカツメガエル遺伝子の改変, 第9回XCIJ-MA・第8回ツメガエル研究会ジョイント研究集会, Nov. 2014.
7.	Minoru Watanabe, Takanori Satoh and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告(第5報), 平成25年度全学FD大学教育カンファレンス in 徳島発表抄録集, 46-47, Dec. 2013.
8.	前高明典, 西山剛司 and Minoru Watanabe : C/EBPα遺伝子による中胚葉遺伝子の発現制御, 第7回日本ツメガエル研究集会要旨集, 20, Sep. 2013.
9.	Minoru Watanabe, Takanori Satoh and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告(第4報), 平成24年度全学FD大学教育カンファレンス in 徳島発表抄録集, 54-55, Dec. 2012.
10.	Minoru Watanabe, Takanori Satoh, 山城考 and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告(第3報), 平成23年度全学FD大学教育カンファレンス in 徳島発表抄録集, 66-67, Jan. 2012.
11.	Minoru Watanabe, 山城考, Takanori Satoh and Makoto Ohashi : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告(第2報), 平成21年度全学FD大学教育カンファレンスin徳島，発表抄録集, 28-29, Mar. 2010.
12.	Minoru Watanabe, 山城考, Takanori Satoh, Makoto Ohashi, 間世田英明, Shyouzo Tsuzuki and Takao Hanabusa : 高大連携事業「高校生の大学研究室への体験入学型学習プログラム」実施報告, 平成20年度全学FD徳島大学教育カンファレンス発表抄録集, 8-9, Jan. 2009.
13.	Minoru Watanabe : 高校生の大学研究室への体験入学ー「ひらめき☆ときめきサイエンス」プログラム報告ー, 平成18年度全学FD徳島大学教育カンファレンス，発表抄録集, 53-54, Mar. 2007.

Report:

1.	Minoru Watanabe, Takanori Satoh and 大橋眞 : 平成27年度全学共通教育授業方法等改善経費使用実績報告書, 1, Tokushima, Mar. 2016.
2.	Takanori Satoh, Makoto Ohashi, Minoru Watanabe, Munkhtsetseg Janlav and Ichinkhorloo Zesem : モンゴル国家畜乳および乳製品の生物資源としての利用を指向した免疫作用および細菌・機能性生体分子の調査研究, 平成24年度学部長裁量経費・創生研究プロジェクト報告書, 20-22, Tokushima, Sep. 2013.
3.	大橋眞, Minoru Watanabe, Takanori Satoh and Hiroyoshi Ishii : 現代GP体験ゼミ吉野川源流の自然と文化を見て・知って・考えてみる, 豊饒な吉野川を持続可能とする共生環境教育実施報告書, 47-51, Tokushima, Mar. 2009.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

転写因子FAST-1標的遺伝子の機能解析による脊椎動物の初期形態形成機構の研究 (Project/Area Number: 16027219 )
Study of the novel Nodal target genes : molecular mechanism of inhibition of the FGF signaling and involvement of mesoderm formation during Xenopus development. (Project/Area Number: 15570172 )
The structural assemble and adaptive evolution of supramolecular hemoglobin (Project/Area Number: 14540629 )
転写因子FAST-1を用いた形態形成遺伝子の網羅的探索と機能解析 (Project/Area Number: 14034253 )
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Professor : Watanabe, Minoru

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)