Tokushima University / Educator and Researcher Directory --- Sakai, Hitomi

https://researchmap.jp/read0201685 (researchmap)

○	Food Science

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Academic Paper (Judged Full Paper):

1.	Hitomi Sakai, Tohru Sakai, Badr Ali Hoida, Kaori Kanemaru and Kumio Yokoigawa : Analysis of cereal extracts as conditioning solutes to suppress the initial attachment of Escherichia coli to abiotic surfaces, European Food Research and Technology, 2020. (Keyword) Cereals / Escherichia coli / Attachment / Abiotic materials (Tokushima University Institutional Repository) ● Metadata: 114680 (Link to Publication Site) ● Publication site (DOI): 10.1007/s00217-020-03444-9 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85081030075 (Tokushima University Institutional Repository: 114680, DOI: 10.1007/s00217-020-03444-9, Elsevier: Scopus)
2.	Tomomitsu Hatakeyama, Ayaka Ichise, Hideaki Unno, Shuichiro Goda, Tatsuya Oda, Hiroaki Tateno, Jun Hirabayashi, Hitomi Sakai and Hideyuki Nakagawa : Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus, Protein Science, Vol.276, No.8, 1574-1583, 2017. (Summary) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure. (Keyword) Amino Acid Sequence / Animal Structures / Animals / Binding Sites / Carbohydrate Sequence / Cercopithecus aethiops / Cloning, Molecular / X-ray crystallography / Escherichia coli / gene expression / Hydrogen Bonding / Lectins / Lymphocytes / Marine Toxins / Mice / Microarray Analysis / Mitogens / Models, Molecular / Polysaccharides / Protein Binding / Protein Interaction Domains and Motifs / Protein Multimerization / Protein Structure, Secondary / Recombinant Proteins / Rhamnose / Sea Urchins / Vero Cells (Link to Publication Site) ● Publication site (DOI): 10.1002/pro.3185 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28470711 ● Summary page in Scopus @ Elsevier: 2-s2.0-85018917301 (DOI: 10.1002/pro.3185, PubMed: 28470711, Elsevier: Scopus)
3.	Hideyuki Nakagawa, Kuniko Nagasaka, Hitomi Sakai, Kozue Edo, Mitsuko Shinohara and kiyoshi Ohura : Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus, International Aquatic Research, Vol.7, No.2, 143-150, 2015. (Link to Publication Site) ● Publication site (DOI): 10.1007/s40071-015-0101-2 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/s40071-015-0101-2 (DOI: 10.1007/s40071-015-0101-2)
4.	Hitomi Sakai, Kozue Edo, Hideyuki Nakagawa, Mitsuko Shinohara, Rie Nishiitsutsuji and Kiyoshi Ohura : Isolation and partial characterization of a L-rhamnose-binding lectin from the globiferous pedicellariae of the toxopneustid sea urchin, Toxopneustes pileolus, International Aquatic Research, Vol.5, No.12, 1-10, 2013. (Keyword) Sea urchin / Toxopneustes pileolus / L-rhamnose-binding lectin / Mitogenic activity / Helper T cell (Link to Publication Site) ● Publication site (DOI): 10.1186/2008-6970-5-12 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1186/2008-6970-5-12 (DOI: 10.1186/2008-6970-5-12)
5.	Kozue Edo, Hitomi Sakai, Hideyuki Nakagawa, Tamiko Hashimoto, Mitsuko Shinohara and Kiyoshi Ohura : Immunomodulatory activity of a pedicellarial venom lectin from the toxopneustid sea urchin, Toxopneustes pileolus, Toxin Reviews, Vol.31, No.3-4, 54-60, 2012. (Keyword) toxopneustid sea urchin / venom / D-galactose-binding lectin / inflammatory cytokine / Th-related cytokine (Link to Publication Site) ● Publication site (DOI): 10.3109/15569543.2012.730090 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84869470363 (DOI: 10.3109/15569543.2012.730090, Elsevier: Scopus)
6.	Kuniko Nagasaka, Hideyuki Nakagawa, Fumihiko Satoh, Takefumi Hosotani, Kumio Yokoigawa, Hitomi Sakai, Haruhiko Sakuraba, Toshihisa Ohshima, Mitsuko Shinohara and Kiyoshi Ohura : A novel cytotoxic protein, Karatoxin, from the dorsal spines of the the redfin velvetfihi, Hypodytes rubripinnis, Toxin Reviews, Vol.28, No.4, 260-265, 2009. (Keyword) Hypodytes rubripinnis / Karatoxin (Link to Publication Site) ● Publication site (DOI): 10.3109/15569540903348940 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-72049118862 (DOI: 10.3109/15569540903348940, Elsevier: Scopus)
7.	Yoko Sakanashi, Keisuke Oyama, Hiroko Matusi, Toshihisa Oyama, Tomohiro Oyama, Yumiko Nishimura, Hitomi Sakai and Yasuo Oyama : Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca²⁺: A model experiment, Life Sciences, Vol.83, No.5-6, 164-169, 2008. (Summary) Quercetin is known to protect the cells suffering from oxidative stress. However, the oxidative stress elevates intracellular Ca2+ concentration, one of phenomena responsible for cell death. Therefore, we hypothesized that quercetin would protect the cells suffering from overload of intracellular Ca2+. To test the hypothesis, the effects of quercetin on the cells suffering from oxidative stress and intracellular Ca2+ overload were examined by using a flow cytometer with appropriate fluorescence probes (propidium iodide, fluo-3-AM, and annexin V-FITC) and rat thymocytes. The concentrations (1-30 µM) of quercetin to protect the cells suffering from intracellular Ca2+ overload by A23187, a calcium ionophore, were similar to those to do so the cells from oxidative stress by H2O2. The cell death respectively induced by H2O2 and A23187 was significantly suppressed by removal of external Ca2+. Furthermore, quercetin greatly delayed the process of Ca2+-dependent cell death although it did not significantly affect the elevation of intracellular Ca2+ concentration by H2O2 and A23187, respectively. It is concluded that quercetin can protect the cells from oxidative injury in spite of increased concentration of intracellular Ca2+. Results suggest that quercetin is also used for protection of cells suffering from overload of intracellular Ca2+. (Keyword) calcium (Link to Publication Site) ● Publication site (DOI): 10.1016/j.lfs.2008.05.009 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18586279 ● Search Scopus @ Elsevier (PMID): 18586279 ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2008.05.009 (DOI: 10.1016/j.lfs.2008.05.009, PubMed: 18586279)
8.	Hideyuki Nakagawa, Fumihiko Satoh, Hitomi Sakai, Hiromi Hayashi and Yasuhiro Ozeki : Biological activities of multiple lectins from the toxopneustid sea urchins, Recent Advances in Marine Science and Technology 2002, 145-152, 2003. (Summary) ラッパウニ科のラッパウニおよびシラヒゲウニの毒棘のタンパク画分をゲル濾過，アフィニティークロマトグラフィーおよびHPLCにより精製し，レクチンを単離し，それらの生物活性を比較検討した．ラッパウニから精製された32 kDa のSUL-I は多様な生物活性を示したが，SUL-II はほとんど細胞増殖活性などの生理活性を示さなかった．一方，シラヒゲウニから精製された23 kDa のヘパリン結合性のレクチン，TGL-I も細胞増殖活性は示さなかった．SUL-I とSUL-II は部分アミノ酸配列において殆ど相同性は認められなかった． (Keyword) sea urchin / lectin / mitogenic activity / amino acid
9.	Hiromi Hayashi, Hitomi Sakai, Wakako Minakuchi-Fijiwara, Miki Takayama, Michiko Nakamura-Murata, Yoshiko Kamo, Kanako Funakoshi, Keisuke Fukumoto, Kaori Kanemaru, Hideyuki Nakagawa, Yasuo Oyama, Nobuyuki Shinohara and Yoshihiro Ito : Cytokinesis arrest and nuclear fission in low density populations of Trichomonad Protozoan, Zoological Science, Vol.19, No.10, 1089-1094, 2002. (Summary) Cell growth of anaerobic protozoan Tritrichomonas foetus was analyzed. This protozoan usually proliferates in extremely high density, but protozoan parasites were dispersed uniformly in F-bouillon medium and cell division stopped temporarily. However, nuclear fission continued and giant polynucleated cells formed. Later, cell division resumed and cells returned to normal form. In conditioned medium, cytokinesis of the dispersed parasites did not stop. Results indicated that T. foetus cells secreted an extracellular factor that influenced cytokinesis. (Keyword) Animals / Cell Division / Cell Nucleus / Culture Media / Oxygen / Population Density / Time Factors / Trichomonas (Link to Publication Site) ● Publication site (DOI): 10.2108/zsj.19.1089 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12426470 ● CiNii @ National Institute of Informatics (CRID): 1522825128326665984 ● Search Scopus @ Elsevier (PMID): 12426470 ● Search Scopus @ Elsevier (DOI): 10.2108/zsj.19.1089 (DOI: 10.2108/zsj.19.1089, PubMed: 12426470, CiNii: 1522825128326665984)
10.	Yasuo Oyama, Hitomi Sakai, Tomohiro Arata, Yoshiro Okano, Norihiko Akaike, Kei Sakai and Katuhiko Noda : Cytotoxic effects of methanol, formaldehyde, and formate on dissociate rat thymocytes: a possibility of aspartame toxicity, Cell Biology and Toxicology, Vol.18, No.1, 43-50, 2002. (Keyword) formaldehyde (Link to Publication Site) ● Publication site (DOI): 10.1023/A:1014419229301 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11991085 ● Search Scopus @ Elsevier (PMID): 11991085 ● Search Scopus @ Elsevier (DOI): 10.1023/A:1014419229301 (DOI: 10.1023/A:1014419229301, PubMed: 11991085)
11.	Yoshihiko Okada, Yasuo Oyama, Lumi Chikahisa, Kaori Kanemaru, Hitomi Sakai, Katuhiko Noda and Masaya Satoh : Tri-n-butyltin-induced decrease in cellular level of glutathione in rat thymocytes: a flow cytometric study using 5-chloromethylfluorescein diacetate, Toxicology Letters, Vol.117, 123-128, 2000. (Keyword) tri-n-butyltin
12.	Hideyuki Nakagawa, Chieko Yamaguchi, Hitomi Sakai, Kaori Kanemaru, Hiromi Hayashi, Yasutetsu Araki, Yasuhiro Tomihara, Mitsuko Shinohara, Kiyoshi Ohura and Hisato Kitagawa : Biochemical and physiological properties of pedicellarial lectins from the toxopneustid sea urchins, Journal of Natural Toxins, Vol.8, No.3, 297-308, 1999. (Summary) Pedicellarial lectins (SUL-I, SUL-II, and TGL-I) were purified from the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla using gel filtration chromatography, affinity chromatography, and reverse-phase HPLC. SUL-I (Nakagawa et al., 1996) and SUL-II from the large globiferous pedicellariae of T. pileolus are D-galactose-binding lectins with molecular masses of 32 kDa and 23 kDa, respectively; while TGL-I from the globiferous pedicellariae of T. gratilla is a Ca(2+)-independent heparin-binding lectin with a molecular mass of 23 kDa. SUL-I induced mitogenic stimulation on murine splenocytes but TGL-I did not. At higher dose ranges SUL-I exhibited inhibitory effects on the cells. The dual response to SUL-I was effectively inhibited by D-galactose. SUL-I enhanced norepinephrine-induced contraction of isolated rat mesenteric artery with endothelium. When endothelium was removed from the artery, acetylcholine did not relax the norepinephrine-induced contraction. In the same artery the enhancing effect of the contraction by SUL-I was abolished, suggesting that SUL-I acts on the endothelium of mesenteric artery, and may release prostanoids. The present results suggest an extracellular function for SUL-I that may have wide-ranging effects in physiological processes. The primary role of pedicellarial lectins from T. pileolus and T. gratilla might be defense against a foreign body. (Keyword) Toxopneustes pileolus / Toxopneustes gratilla / D-galactose-specific lectin / Heparin-specific lectin / Contraction (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10591033 ● Search Scopus @ Elsevier (PMID): 10591033 (PubMed: 10591033)

Proceeding of International Conference:

1.	Hideyuki Nakagawa, Kozue Edo, Hitomi Sakai, Yuika Watanabe, Mitsuko Shinohara and Kiyoshi Ohura : Mining on novel lectins from some venomous fish and sea urchins, Abstract of 9th IST Asia Pacific Meeting on Animal, Plant and Microbial Toxins, 10, Vladivostok, Russia, Sep. 2011. (Keyword) D-galactose-binding lectin / venomous fish / sea urchin venom / Karatoxin / dendritic cell
2.	Natsuko Kusunoki, Kozue Edo, Kaori Ohnishi, Keiko Ashida, Asami Nakanishi, Hitomi Sakai, Hideyuki Nakagawa, Kumio Yokoigawa, Haruhiko Sakuraba and Toshihisa Ohshima : Multiple lectins from globiferous pedicellariae of the sea urchin, Toxopneustes pileolus, The Proceeding of 21st Pacific Science Congress:Session:4-10 Health hazard by marine animals in Pacific region, 7p, Okinawa, Jun. 2007. (Keyword) lectin / sea urchin / venom / Toxopneustes pileolus / pedicellariae
3.	Hideyuki Nakagawa, Hitomi Sakai, Kaori Kanemaru and Hiromi Hayashi : A sea urchin lectin, SUL-I induces differentiation on murine myeloid leukemic cells, GRC on Marine Natural Products, Ventura, California, Feb. 2004. (Summary) ラッパウニ叉棘から精製されたD-galactose binding lectin のSUL-I はマウス由来のmyeloid leikemic cell をマクロファージ様に分化誘導し，部分的なアポトーシスを引き起こした． (Keyword) murine myeloid cells / differentiation / D-galatose-binding lectin / sea urchin lectin / Toxopneustes pileolus
4.	Hideyuki Nakagawa, Fumihiko Satoh, Hitomi Sakai, Hiromi Hayashi and Yasuhiro Ozeki : Biological activities of multiple lectins from the toxopneustid sea urchins, 10th Pacific Congress on Marine Science and Technology, Chiba, Jun. 2002. (Summary) We isolated two D-galactose-binding lectins (SUL-I and SUL-II) and a heparin-binding lectin (TGL-I) from Toxopneustes pileolus and Tripneustes gratilla, respectively. (Keyword) toxopneustid sea urchins / lectins / D-galactose / mitogenic activity / chemotaxis
5.	Hideyuki Nakagawa, Hitomi Sakai, Hiromi Hayashi, Yasuhiro Tomihara and Yasutestsu Araki : Some physiological studies of pedicellarial lectins from the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla, 6th International Symposium: Protein Structure Function Relationship, Karachi, Pakistan, Feb. 1999. (Summary) ラッパウニ科のラッパウニおよびシラヒゲウニの叉棘からレクチンを精製し，生物活性について比較検討した． (Keyword) Toxopneustes pileolus / Tripneustes gratilla / Pdeicellariae / Mitogenic activity
6.	Hitomi Sakai, Rie Konii, Wakako Minakuchi, Miki Takayama, Hideshi Takatani, Fukiyo Tomiyoshi and Hiromi Hayashi : Observation of multinucleated cells of Trichomonas foetus in in vitro culture, IXth International Congress of Parasitology, 296, Chiba, Aug. 1998. (Keyword) polynucleated cell / trichomonad protozoan / nuclear fission / cell division / cell cycle

Proceeding of Domestic Conference:

1.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 身の回りの素材に対する小麦粉由来アラビノキシランの大腸菌付着抑制効果について, 第43回日本食品微生物学会, Sep. 2022.
2.	Hitomi Sakai, Kaori Kanemaru, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着を抑制する小麦粉の成分の同定, 第39回日本食品微生物学会総会, Sep. 2018.
3.	Kaori Kanemaru, Hitomi Sakai, Keiko Takahashi and Kumio Yokoigawa : ガラス表面に付着した大腸菌の洗浄と殺菌, 第39回日本食品微生物学会学術総会講演要旨集, 111, Sep. 2018. (Keyword) biofilm / Escherichia coli
4.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着を抑制する小麦粉の成分について, 第38回日本食品微生物学会学術総会, Oct. 2017.
5.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 調理器具素材への大腸菌の付着に及ぼす穀類の影響, 第37回日本食品微生物学会学術総会講演要旨集, 111, Sep. 2016.
6.	Hitomi Sakai, Tohru Sakai and Kumio Yokoigawa : 大腸菌の非生物素材への付着に対する穀類の影響, 日本農芸化学会中四国支部第45回講演会要旨集, 23, Jun. 2016.
7.	Hitomi Sakai, Kozue Edo, Maoko Yoshino, Hideyuki Nakagawa, Mitsuko Shinohara and Kiyoshi Ohura : Isolation of novel lectins from the globiferous pedicellariae of the toxopneustes sea urchin, Toxopneustes pileolus, 88th JPS Annual Meeting Program, 214p, Feb. 2015. (Keyword) sea urchin / venom / lectin / mitogen activity / agglutinating activity
8.	Edo Kozue, Hitomi Sakai, Yoshino Maoko and Hideyuki Nakagawa : 徳島産ラッパウニの叉棘に由来するレクチンの多様性について, 日本薬理学会西南部会プログラム・抄録集, 52, Nov. 2014. (Keyword) sea urchin / pedicellaria / venom / lectin / diversity
9.	江戸梢, Hitomi Sakai, Hideyuki Nakagawa, Naoyoshi Nishibori and 西尾幸郎 : 徳島産ラッパウニの叉棘レクチンの生理活性について, 日本農芸化学会2014年度中四国支部大会(第40回公演会)講演要旨集, 49, Sep. 2014. (Keyword) ラッパウニ / ウニ毒 / レクチン / 凝集反応 / マイトジェン活性
10.	Hideyuki Nakagawa, Kozue Edo, Yuri Kotake, Hitomi Sakai, Mitsuko Shinohara and Kiyoshi Ohura : Isolation of a novel mitogenic factor from the pedicellariae of the toxopneustid sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.121, No.Supplement 1, 157P, Mar. 2013. (Keyword) sea urchin / Toxopnesustes pileolus / mitogenic activity / mitogen / venom
11.	Hitomi Sakai, Hideyuki Nakagawa, kouzue Edo, Mitsuko Shinohara and Kiyoshi Ohura : ラッパウニの卵巣に由来するレクチンの探索, Folia Pharmacologica Japonica, Vol.140, No.4, 9P, Oct. 2012. (Keyword) sea urchin / Toxopneustes pileolus / ovary / bioactive substance / lectin
12.	Kozue Edo, Hitomi Sakai and Hideyuki Nakagawa : 徳島産ラッパウニの大型叉棘に由来するマイトジェン様物質の精製について, (社)日本家政学会中国・四国支部研究発表要旨集 2012, 9, Oct. 2012. (Keyword) sea urchin / Toxopneustes pileolus / mitogen / aimon acid sequence / chemotaxis
13.	Kozue Edo, Shizuka Satoh, Hitomi Sakai and Hideyuki Nakagawa : Diversity of bioactive substances from the globiferous pedicellariae of the sea urchin, Toxopneustes pileolus, 社団法人日本家政学会第64回大会研究発表要旨集, 119, May 2012. (Keyword) Toxopneustes pileolus / bioactive substance / galectin-3 / mitogen / sea urchin lectin
14.	Hideyuki Nakagawa, Jun Kurochi, Kozue Edo, Yuika Watanabe, Hitomi Sakai, Ayaka Miki, Shun Morikawa, Mitsuko Shinohara and Kiyoshi Ohura : Partial purification and characterization of a glycoprotein from the coelomic fluid of the sea urchin, Toxopneustes pileolus, Journal of Pharmacological Sciences, Vol.118, No.Suppl. 1, 258p, Feb. 2012. (Keyword) coelomic fluid / galectin-3 / Toxopneustes pileolus / agglunination / mitogenic response
15.	江戸梢, 橋本多美子, Hitomi Sakai and Hideyuki Nakagawa : 徳島産ラッパウニの叉棘に由来するレクチンのサイトカイン産生と遊走活性, 第58会(社)日本家政学会中国・四国支部研究発表会研究発表要旨集, 12, Oct. 2011. (Keyword) sea urchin / lectin / chemotaxis / inflammatory cytokine / pedicellaria
16.	Kozue Edo, Tamiko Hashimoto, Hitomi Sakai and Hideyuki Nakagawa : Biological activity and diversity of the pedicellarial lectin from the toxopneustid sea urchin, Toxopneustes pileoluse, (社)日本家政学会第63回大会研究発表要旨集, 141, May 2011. (Keyword) sea urchin / lectin / pedicellariae / diversity / biological activity / sea urchin venom
17.	Hideyuki Nakagawa, kozue Edo, Yuika Watanabe, Hitomi Sakai, Mitsuko Shinohara and Kiyoshi Ohura : Biological activites of a novel lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, Journal of Pharmacological Sciences, Vol.115, No.Suppl. 1, 231p, Mar. 2011. (Keyword) Hypodytes rubripinnis / Dorsal spine / galectin-3 / Mitogenic activity / Chemotactic activity
18.	Kozue Edo, Hideyuki Nakagawa, Hitomi Sakai, Tamiko Hashimoto, Jun Kurochi, Masahiro Sakata, Shizuka Satoh, Mitsuko Shinohara, Kiyoshi Ohura and Haruhiko Sakuraba : Biological activities of a novel glycoprotein, Karatoxin, from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, 第83回日本生化学会大会プログラム, 380, Dec. 2010. (Keyword) scorpionfish / redfin velvetfish / venom / lectin
19.	江戸梢, 橋本多美子, Hitomi Sakai and Hideyuki Nakagawa : ラッパウニ科のウニに由来する糖結合性タンパク質の性質について, 第57回日本家政学会中国・四国支部研究発表要旨集, 13, Oct. 2010. (Keyword) ウニ / レクチン / D-ガラクトース / 細胞増殖活性
20.	Hitomi Sakai, 香山暎里 and Hiromi Hayashi : 多核Trichomonas foetus細胞の分裂過程の解析第39回日本原生動物学会, Nov. 2006.
21.	河野直子, 細谷岳史, 宮田匡志, 萱野真弓, Hideyuki Nakagawa, Hitomi Sakai, Hiromi Hayashi, 野崎真敏, 篠原光子, 大浦清 and 長坂壽訓 : Partial purification of novel lectins from the dorsal spines of the reef stonefish, Synanceia verrucosa, Journal of Pharmacological Sciences, Vol.97, 278, Mar. 2005.
22.	Hiromi Hayashi and Hitomi Sakai : Cell growth of parasitic protozoan Trichomonas foetus in low cell density culture, 第72回日本寄生虫学会大会, Mar. 2003.
23.	Hideshi Takatani, Hitomi Sakai and Hiromi Hayashi : Elimination of the intraperitoneal parasites in mice injected with Trichomonas foetus cells, 第33回日本原生動物学会大会, Nov. 2000.
24.	Hitomi Sakai, Rie Konii, Wakako Minakuchi, Fukiyo Tomiyoshi and Hiromi Hayashi : Observation of multinuclear cells of Trichomonas foetus in in vitro culture, Apr. 1998. (Keyword) Trichomonas foetus / multinuclear cell
25.	Hitomi Sakai, Kana Hamaguchi, Keisuke Fukumoto and Hiromi Hayashi : Appearance of multinuclear cells of Trichomonas foetus in in vitro culture, Oct. 1996. (Keyword) Trichomonas foetus / multinuclear cell

Report:

1.	Hideyuki Nakagawa, Chieko Yamaguchi, Hitomi Sakai and Hiromi Hayashi : 沖縄産ガンガゼの毒棘タンパク画分のマイトジェン活性について, 平成9年度海洋危険性生物対策事業報告書, 27-31, Naha, Oct. 1998. (Summary) ガンガゼ毒棘の抽出標品は強い血球凝集活性を示した．抽出標品をゲル濾過法で分画し，血球凝集活性と細胞増殖活性を測定した．細胞増殖活性は100倍上昇した．この画分による血球凝集反応はD-ガラクトサミンで弱い阻害を受けた．SDS-PAGE では同画分は20 kDa の位置にタンパクバンドを示した． (Keyword) Sea urchin / Diadema setosum / Lectin / Mitogenic activity / Toxin
2.	Hideyuki Nakagawa, 山口知栄子, Hitomi Sakai, Hiromi Hayashi, 富原靖博 and 新城安哲 : 沖縄産ウニ類の棘および叉棘タンパク画分のマイトジェン活性, 平成8年度海洋性危険生物対策事業報告書, 23-27, Naha, Mar. 1997. (Summary) 沖縄本島沿海で採集したシラヒゲウニ，ガンガゼおよびリュウキュウフクロウニの棘抽出蛋白質画分の血球凝集活性ならびに細胞増殖活性を検討した．ウサギ血液では，3種類ともに強い凝集活性を示した．この結果から，3種の抽出標品にはレクチンが存在することが推定された，そこで細胞増殖活性を検討した．シラヒゲウニ，ガンガゼおよびリュウキュウフクロウニ抽出標品は強い活性を示した．この結果は，3種類のウニは生理活性物質としてのレクチンの宝庫の可能性を示唆した． (Keyword) Lectin / Sea urchins / Tripneustes gratilla / Diadema setosum / Asthenodoma sp. / mitogenic activity

食品の新機能開拓と革新的食中毒予防の開発 (Project/Area Number: 21K13499 )
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Technician : Sakai, Hitomi

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