Tokushima University / Educator and Researcher Directory --- Suzuki, Yoshihisa

Personal Web Page

https://researchmap.jp/read0070755 (researchmap)

Field of Study

○	Crystal Growth

Subject of Study

○	Crystallization of Biological Macromolecules, Colloidal crystal, Microgravity experiments, Synchrotron X-ray Crystallography of Protein Crystals (Protein crystallization, colloidal crystal, Microgravity experiments, Synchrotron X-ray Crystallography of Protein Crystals, Liquid-liquid Phase Separation of Protein Solutions)

Book / Paper

Book:

1.	Yoshihisa Suzuki, Ai Ninomiya and Shin-ichiro Yanagiya : The 3rd International Conference on Nanomaterials and Advanced Composites, --- Chapter 8 In Situ Observation of Crystal Growth Processes ---, Springer Nature, Singapore, Nov. 2023.
2.	Yoshihisa Suzuki : タンパク質の構造解析手法と In silico スクリーニングへの応用事例 ∼AlphaFold，In silico 創薬， NMR，X 線，クライオ電子顕微鏡∼ 第 3 章高品質なタンパク質結晶の求め方第 4 節塩濃度の違いによるタンパク質結晶の構造解析, Technical Information Institute Co., Ltd., Jul. 2023. (Keyword) Concentration / protein / crystal growth
3.	Yoshihisa Suzuki : 高圧力の科学・技術事典 VII章生物関連科学(編集:加藤稔・松木均) 1 生体高分子 6 高圧結晶成長, 朝倉書店, Tokyo, Nov. 2022.
4.	Yoshihisa Suzuki : Modern Aspects of Bulk Crystal and Thin Film Preparation, --- Chapter 19 Protein Crystal Growth Under High Pressure ---, Intech, Rijeka, Jan. 2012.
5.	Emi Konda, Yoshihisa Suzuki, Hironori Hondoh and Katsuhiro Tamura : 高圧力下の生物科学: 第7章リゾチーム三斜晶系結晶の溶解度に及ぼす温度・圧力・pHの効果, さんえい出版株式会社, Kyoto, Aug. 2006.
6.	浅井俊高, Yoshihisa Suzuki, 竹重和也 and Katsuhiro Tamura : 高圧力下の生物科学: 第6章リゾチーム単斜晶系結晶の成長速度に及ぼす圧力効果, さんえい出版株式会社, Kyoto, Aug. 2006.
7.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : 高圧力下の生物科学: 第8章タンパク質結晶表面のステップ前進速度に及ぼす圧力効果, さんえい出版株式会社, Kyoto, Aug. 2006.
8.	山本忠, Katsuhiro Tamura and Yoshihisa Suzuki : 高圧力下の生物科学, さんえい出版株式会社, Kyoto, Aug. 2006.
9.	Katsuhiro Tamura, 松本知浩, Toshiaki Arao and Yoshihisa Suzuki : 生物科学·食品科学への高圧利用(再掲), さんえい出版株式会社, Kyoto, Aug. 2003. (Summary) 新しく製作したガス圧高圧熱量計の利用分野の紹介ならびに具体的な研究例についての解説．1つの研究例は「リポソーム二分子膜の相転移温度に及ぼすガス圧力の影響」に関するもので，Dimyristoyl phosphatikylcholine 二分子膜の相転移温度を酸素，窒素，ヘリウム，アルゴン，亜酸化窒素などの気体で加圧したところ，気体の性質に応じて様々な挙動を示した．他の研究例は「微生物の代謝熱測定によるバイオアッセイ」に関するもので，水圏，気圏，いずれの試料でも定量が可能なバイオアッセイの確立を目指している．
10.	Toshiaki Arao, Yoshihisa Muramoto, Yoshihisa Suzuki and Katsuhiro Tamura : Advances in High Pressure Bioscience and Biotechnology II, --- Effects of High-Pressure Gas on the Growth Thermograms of Yeast ---, Springer-Verlag, Berlin Heidelberg New York, Jun. 2003. (Summary) 高圧マイクロ熱量計を使用して，ガス圧力下における酵母の増殖サーモグラム(代謝熱-培養時間曲線)を描いた．液体培地を窒素，空気，亜酸化窒素，アルゴンで直接加圧することにより，成長阻害効果が現れた．得られた熱データをもとに気体の毒性指標として最小阻止圧力(MIP)を決定した．毒性は以下のような順番となった．空気>亜酸化窒素>アルゴン>窒素．
11.	Yoshihisa Suzuki, Hiroshi Suto, Tsutomu Sawada and Katsuhiro Tamura : Advances in High Pressure Bioscience and Biotechnology II, --- Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals ---, Springer-Verlag, Berlin Heidelberg New York, Jun. 2003. (Summary) 斜方晶系リゾチーム結晶の溶解度と成長速度を0.1, 100 MPaで測定した．溶解度は，結晶の成長·溶解速度を溶液濃度を変えて測定し，成長·溶解速度が0となるときの溶液濃度として決定した．その結果，溶解度は30, 35, 40℃において，加圧に伴い減少した．これは，加圧に伴い過飽和度が増加し，結晶化が進むことを意味する．(010)面の結晶成長速度R(010)を過飽和度σ=ln(C/Ce) (C:溶液濃度 Ce: 溶解度) に対してプロットし，kinetic係数k=R(010)/σを見積もった．kは，30, 35, 40℃で加圧に伴い増加した．これは，加圧に伴い成長kineticsも促進されたことを意味する．
12.	Katsuhiro Tamura, Matsumoto Tomohiro and Yoshihisa Suzuki : Advances in High Pressure Bioscience and Biotechnology II, --- Effects of Gas Pressure on the Phase Transition Temperature of Liposomal Bilayer Membranes ---, Springer-Verlag, Berlin Heidelberg New York, Jun. 2003. (Summary) リン脂質二分子膜(DMPC, DPPC)の主相転移温度(Tm)を様々なガス圧の下で決定した．使用したガスは酸素，窒素，水素，ヘリウム，アルゴンといった単体のガスと，空気，Heベースの20%酸素, 窒素ベースの10%亜酸化窒素などの複合ガスである．加圧によるTmの振る舞いは二つのグループに分類できた．1つは，Tmは圧力増加とともに直線的に増加したが，その他のグループでは10-20 MPaまでの圧力範囲で減少した．これらの原因の1つは，気体の水への溶解度の違いによる．脂質への気体の溶解度も，重要な因子になり得る．
13.	Yoshihisa Suzuki, Satoru Miyashita, Tsutomu Sawada, Gen Sazaki, Toshitaka Nakada, Hiroshi Komatsu, Toshiaki Arao and Katsuhiro Tamura : Progress in Biotechnology Vol. 19: Trends in High Pressure Bioscience and Biotechnology, --- Effects of pressure on growth kinetics of protein crystals ---, Elsevier Science, Jan. 2002. (Summary) 0.1, 50, 100 MPaの圧力下で正方晶系ニワトリ卵白リゾチーム結晶の{110}, {101}面の面成長速度をその場観察した．高圧力下での溶解度は高圧力下での過飽和度の決定を可能にする．同じ過飽和度でも，両方の結晶面とも加圧に伴い成長速度が減少した．これは，表面成長kineticsが圧力に依存することを意味している．birth and spreadモデルを使って成長kineticsにおよぼす圧力効果について理解した．加圧に伴う平均レッジ表面自由エネルギーの増加によって，成長速度の減少が説明できた．また，100 MPa1時間の加圧を施した5mg/mlのリゾチームのモル熱変性エンタルピーを示差走査型熱量計を用いて測定した．その結果，未加圧の試料と変わらなかったので，本研究における100 MPaまでの圧力による不可逆変性の影響は無視出来た．
14.	Kazuhiro Hisada, Yoshihisa Suzuki and Katsuhiro Tamura : Progress in Biotechnology Vol. 19: Trends in High Pressure Bioscience and Biotechnology, --- Thermotolerance and barotolerance of alcohol-shocked yeast ---, Elsevier Science, Jan. 2002. (Summary) 8種類のアルコールを用いてアルコールショックを加えた酵母菌のストレス耐性に関する研究．アルコールショック処理により酵母に高温，高圧力や薬物に対するストレス耐性が誘導された．最大耐性を誘導する最適アルコールショック濃度は，アルコールの物性に依存した．この最適濃度はアルコール分子の炭素数に逆比例した．さらに，アルコール分子の水酸基の位置はストレス耐性の強さに影響を及ぼすことが分かった．また，マイクロカロリメトリーにより，酵母の増殖に伴う発熱曲線からアルコールの最小阻止濃度(MIC)を決定し，アルコールショックの最適アルコール濃度との関係を考察している．
15.	Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Progress in Biotechnology Vol. 19: Trends in High Pressure Bioscience and Biotechnology, --- Effects of saccharide in medium on stress tolerance of yeast ---, Elsevier Science, Jan. 2002. (Summary) 酵母のストレス耐性に及ぼす培地中の糖類(glucose, fructose, galactose, mannose, lactose, maltose, sucrose, trehalose)の影響をコロニーカウント法で調べた．高圧力と高温に対する酵母細胞における様々な糖類の防御効果を，生存率を用いて評価した．糖の添加は高温や高圧に対する酵母の生存率を明らかに増加させた．中でもtrehaloseは酵母細胞に対して最大の圧力耐性を導付与した．また二糖類は単糖類に比べてより高い耐性を付与した．高圧力·高温に対する糖類の防御効果は，糖類分子のエカトリアルOH基の数によって説明された．
16.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Hiroshi Komatsu, Toshiaki Arao and Katsuhiro Tamura : Progress in Biotechnology Vol. 19: Trends in High Pressure Bioscience and Biotechnology, --- In situ measurements of the solubility of protein crystals under high pressure ---, Elsevier Science, Jan. 2002. (Summary) ニワトリ卵白リゾチーム正方晶系結晶に及ぼす圧力効果をマッハツェンダー干渉計と，高圧光学セルを用いてその場観察により測定した．結晶の平衡化過程による濃度変化過程を，過飽和状態·未飽和状態の両方から正確にかつ連続的に測定した．一週間程で一定の値に到達したが，両者は一致しなかった．理論的な観点から，未飽和状態から到達した濃度を平衡濃度とした．また，この実験の条件でのタンパク質の圧力変性の有無をモルエンタルピーの測定から確認した結果，実験中の変性はないという事が明らかになった．

Academic Paper (Judged Full Paper):

1.	Yoshihisa Suzuki and Kohei Hosokawa : Very high solubility and low enthalpy of dissolution of orthorhombic lysozyme crystals grown in deionized water, Journal of Crystal Growth, Vol.630, 127600-1-127600-4, 2024. (Summary) Solubility data are indispensable to control the quality of protein crystals. The solubility curves of orthorhombic hen egg-white lysozyme (HEWL) crystals grown in deionized water (salt-free crystals) were measured in this study. Once salt-free crystals have been successfully grown, we can measure solubility with arbitrary salt concentrations, including in vivo. The absolute values of the solubilities of the salt-free crystals were found to be the largest of all HEWL crystals grown under various high salt concentration conditions. Van t Hoff plots of the solubilities of the salt-free crystals showed the lowest enthalpy of dissolution (15 ± 2 kJ mol-1). (Keyword) Biocrystallization / solubility / Growth from solutions / Lysozyme / Proteins (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2024.127600 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2024.127600 (DOI: 10.1016/j.jcrysgro.2024.127600)
2.	Ryusei Fukunaga, Masahide Sato and Yoshihisa Suzuki : In-situ observation of homogeneous nucleation of colloidal crystals formed with attractive interactions under density-matching conditions, Modern Physics Letters. B, in-press, 2024. (Summary) Colloidal crystals formed with attractive interactions are used for in-situ observations ofhomogeneous nucleation processes in density-matched dispersion.We found globular and plate-likepolycrystalline clusters stably suspended in the dispersion. Both clusters rotated randomlyand moved freely with suspending in the dispersion. They were probably formed through homogeneous nucleation processes. (Keyword) colloidal crystal / nucleation / depletion aggregation / heavy water (Link to Publication Site) ● Publication site (DOI): 10.1142/S0217984924410173 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1142/S0217984924410173 (DOI: 10.1142/S0217984924410173)
3.	Yoshihisa Suzuki, Akiho Ikeuchi, Koichiro Taoka and Gen Sazaki : Solution-mediated phase transition of protein crystals, Modern Physics Letters. B, in-press, 2024. (Summary) A snapshot of the evidence of solution-mediated phase transition of glucose isomerase (GI)crystals more than 7 months after the start of an experiment was successfully captured. The transition to P21212 crystals started about 2 months after the first I222 crystals nucleated. To conduct such long-term experiments, we developed novel observation cells with liquid paraffin layers to prevent aqueous GI solutions from evaporation of water. Changes in the weights of cells were measured, and much less evaporation was successfully achieved by continuously adding liquid paraffin as a liquid sealant. (Keyword) in situ observation / Solution-mediated phase transition / glucose isomerase / evapolation (Link to Publication Site) ● Publication site (DOI): 10.1142/S021798492441015X (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1142/S021798492441015X (DOI: 10.1142/S021798492441015X)
4.	Masahiro Kanno, Yutaka Tsuboi, Shigeki Matsuo and Yoshihisa Suzuki : Activation energy of kink incorporation of particles into colloidal crystals with attractive interactions, CrystEngComm, Vol.25, No.12, 1828-1832, 2023. (Summary) The kink incorporation and diffusion of particles on the growth interface of colloidal crystals with attractive interactions between particles are important elementary processes. We have successfully estimated the activation energy of kink incorporation, εkink, and vibrational frequency, ν, of silica particles on the growth interface using two fundamentally different approaches. One was the use of the Arrhenius plot of step velocities, Vstep, at five temperatures. The other was the use of ν measured by the direct observation of particle movements with a high-speed camera. Although ν and εkink took similar values for both approaches, the errors of ν and εkink are smaller for the direct observation case than those obtained by the Arrhenius plot. This study is the first to estimate quantitively the activation energy of kink incorporation and the vibrational frequency of particles on the growth interface of a colloidal crystal. These findings would contribute significantly to the understanding of crystal growth mechanisms. (Keyword) crystal growth / step velocity / colloidal crystal / depletion attraction (Link to Publication Site) ● Publication site (DOI): 10.1039/D2CE01524B (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1039/D2CE01524B (DOI: 10.1039/D2CE01524B)
5.	Yoshihisa Suzuki, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Taro Shimaoka, Masae Nagai, Koji Inaka, Shin-ichiro Yanagiya, Takehiko Sone, Shingo Wachi, Yasutomo Arai and Katsuo Tsukamoto : Highly Purified Glucose Isomerase Crystals Under Microgravity Conditions Grow as Fast as Those on the Ground Do, Crystal Growth & Design, Vol.22, No.12, 7074-7078, 2022. (Summary) Suppression of convection flows (solute transportation) and that of impurity incorporation into crystals seem to be the main reasons why the quality of protein crystals becomes better under microgravity conditions, whereas each precise mechanism has not been completely clarified yet. We tried to clarify the former reason by the in-situ observation of spiral growth hillocks on the {110} faces of highly purified glucose isomerase (GI) crystals under microgravity conditions and on the ground. Lateral growth rates Vlateral of a spiral hillock on the {110} face of a glucose isomerase crystal in situ under microgravity conditions and step velocities Vstep of the same configuration on the ground took similar values as far as the maximum values are compared each other. This similarity indicates there are less influences of the convection flows on the growth rates of protein crystals contrary to conventional expectations. (Keyword) crystal growth / nucleation / glucose isomerase / microgravity (Link to Publication Site) ● Publication site (DOI): 10.1021/acs.cgd.2c00751 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/acs.cgd.2c00751 (DOI: 10.1021/acs.cgd.2c00751)
6.	Yoshihisa Suzuki, Shiori Fujiwara, Shoko Ueta and Takashi Sakai : Precipitant-free Crystallization of Lysozyme and Glucose Isomerase by Drying, Crystals, Vol.12, 129-1-129-8, 2022. (Summary) Protein crystallization is usually conducted by using precipitants, although the salting-out phenomenon is still unclear and complex. Moreover, the addition of precipitants sometimes results in irreversible disordered precipitation of protein molecules. Although precipitant-free lysozyme crystals obtained by centrifugal concentration showed significant changes in three-dimensional structure compared to the structure of salted-out crystals, it was rather difficult to mount crystals from a viscous dense liquid phase after centrifugal concentration, and the quality of the crystals often deteriorated during the mounting process. Here we present novel precipitant-free crystallization methods, which were effective for lysozyme and glucose isomerase. Tetragonal lysozyme crystals were successfully crystallized in a glass capillary simply by drying highly concentrated lysozyme solution in the presence of 0.01 M hydrochloric acid without using any precipitants. Glucose isomerase dissolved in ultra-pure water was also successfully crystallized in hanging drops by drying highly concentrated solution under low-humidity conditions. Oscillation images of the obtained crystals were safely collected without handling; they clearly indicated the crystals had a tetragonal form for lysozyme and an orthorhombic form for glucose isomerase, and their lattice parameters are similar to those of previously reported crystals obtained by salting-out methods. (Keyword) precipitant-free / drying / highly concentrated solution / oscillation image (Tokushima University Institutional Repository) ● Metadata: 116991 (Link to Publication Site) ● Publication site (DOI): 10.3390/cryst12020129 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3390/cryst12020129 (Tokushima University Institutional Repository: 116991, DOI: 10.3390/cryst12020129)
7.	Haruhiko Koizumi, Uda Satoshi, Ryo Suzuki, Masaru Tachibana, Kenichi Kojima, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama and Yoshihisa Suzuki : Control of strain in subgrains of protein crystals by the introduction of grown-in dislocations, Acta Crystallographica. Section D, Structural Biology, Vol.77, 599-605, 2021. (Summary) It is important to reveal the exact cause of poor diffractivity in protein crystals in order to determine the accurate structure of protein molecules. It is shown that there is a large amount of local strain in subgrains of glucose isomerase crystals even though the overall crystal quality is rather high, as shown by clear equal thickness fringes in X-ray topography. Thus, a large stress is exerted on the subgrains of protein crystals, which could significantly lower the resistance of the crystals to radiation damage. It is also demonstrated that this local strain can be reduced through the introduction of dislocations in the crystal. This suggests that the introduction of dislocations in protein crystals can be effective inenhancing the crystal quality of subgrains of protein crystals. By exploiting this effect, the radiation damage in subgrains could be decreased, leading to the collection of X-ray diffraction data sets with high diffractivity. (Link to Publication Site) ● Publication site (DOI): 10.1107/S2059798321001820 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85105484744 (DOI: 10.1107/S2059798321001820, Elsevier: Scopus)
8.	Yohei Yamada, Takahito Suzuta, Eriko Okada, Toshio Takayanagi, Yoshihisa Suzuki, Kei-ichiro Murai and Tomoki Yabutani : タンパク質結晶成長場及びX線回折実験用試料固定材としてのトラックエッチドメンブランフィルターの利用, Bunseki Kagaku, Vol.68, No.9, 639-646, 2019. (Link to Publication Site) ● Publication site (DOI): 10.2116/bunsekikagaku.68.639 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001277365707776 ● Search Scopus @ Elsevier (DOI): 10.2116/bunsekikagaku.68.639 (DOI: 10.2116/bunsekikagaku.68.639, CiNii: 1390001277365707776)
9.	Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka and Yasutomo Arai : A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature, The Review of Scientific Instruments, Vol.90, 054101-1-054101-4, 2019. (Summary) We have developed a handling-free mounting method for X-ray crystallography ofprotein crystals at room temperaturesthe glass capillary method. In this method,crystals were nucleated and grown on the capillary walls, and then growth solutions weregently removed. The procedures for collecting data on the crystals were conducted bysimply setting the capillary on the goniometer of a synchrotron beamline withouttouching the crystals. Crystal quality was characterized using mosaicity, resolution at I /σ(I) = 2, I / σ(I) at resolution = 2.0 , Rmerge , and Completeness. Wilson plots were alsoused to characterize the quality of crystals. In particular, all samples showed very lowmosaicity; the handling-free method successfully retained their low mosaicity andeffectively maintained the crystal quality. (Keyword) crystal growth / synchrotron radiation / X-ray crystallography / room temperature / mosaicity (Link to Publication Site) ● Publication site (DOI): 10.1063/1.5070122 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1063/1.5070122 (DOI: 10.1063/1.5070122)
10.	Atsushi Mori, Masahide Sato and Yoshihisa Suzuki : Effect of density change at crystallization on a one-dimensional heat balance equation at solid-liquid interface, Japanese Journal of Applied Physics, Part 1 (Regular Papers & Short Notes), Vol.58, 045506-1-045506-6, 2019. (Link to Publication Site) ● Publication site (DOI): 10.7567/1347-4065/ab0707 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85065168560 (DOI: 10.7567/1347-4065/ab0707, Elsevier: Scopus)
11.	Yoshihisa Suzuki, Takahisa Fujiwara, Katsuo Tsukamoto, Seijiro Fukuyama, Taro Shimaoka, Kazuma Tsuchiya, Ai Ninomiya, Yumiko Murakumo, Akiho Ikeuchi, Hitomi Minamizono, Tomokazu Yamazaki, Takehiko Sone, Tetsuya Sakashita, Masae Nagai and Yasutomo Arai : Very low nucleation rates of glucose isomerase crystals under microgravity in the International Space Station, Crystals, Vol.9, No.2, 90-1-8, 2019. (Summary) In situ observation of the nucleation and growth of glucose isomerase (GI) crystals under microgravity was conducted using an optical microscope during the first flight of the Advanced Nano Step project undertaken in the International Space Station (ISS). Very low apparent nucleation rates (J) of GI crystals in the solution and on the substrate of the growth container were confirmed compared with those on the ground. In particular, J of GI crystals in the solution were a few times lower than that on the substrate. The growth rates (R) of the {101} faces of GI crystals in the solution and on the substrate were measured. The very low nucleation rates allowed us to successfully measure R at a very high supersaturation region (up to ln(C/Ce)=6), at which R cannot be measured on the ground. (Keyword) crystal growth / nucleation / growth / glucose isomerase / microgravity (Tokushima University Institutional Repository) ● Metadata: 115075 (Link to Publication Site) ● Publication site (DOI): 10.3390/cryst9020090 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85062873727 (Tokushima University Institutional Repository: 115075, DOI: 10.3390/cryst9020090, Elsevier: Scopus)
12.	Yoshihisa Suzuki, Takahisa Fujiwara, Tomohiro Shiomoto, Tetsuya Toyooka, Daidoh Nakahashi, Katsuo Tsukamoto and Yuko Inatomi : Dependence of Convection Flow Rates on Gravity around Growing Hen Egg-White Lysozyme Crystals, International Journal of Microgravity Science and Application, Vol.35, No.3, 350304-1-350304-5, 2018. (Summary) Convection flows induced by formation of solute depletion zone around growing protein crystals are visualized to confirm whether growth of crystals at a ceiling position (top wall of a growth container) can really suppress this convection flow effectively or not. First, we observed the convection flows around the crystal at the ceiling position at 1.0 G. Second, parabolic flight experiments revealed that flow rates of polystyrene particles (as marker particles, 500 nm in diameter) around a crystal at the ceiling position moved and thus do not indicate zero at high-gravity conditions, and the particles almost stop at zero gravity. Thus, stable microgravity experiments are still indispensable to attain complete convection-free conditions. (Keyword) crystal growth / Convection flow / Solutal convection / Flow rates / Ceiling position (Link to Publication Site) ● Publication site (DOI): 10.15011//jasma.35.350304 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.15011//jasma.35.350304 (DOI: 10.15011//jasma.35.350304)
13.	Liyuan Niu, Kazuki Nomura, Hitoshi Iwahashi, Hiroyuki Matsuoka, Satoshi Kawachi, Yoshihisa Suzuki and Katsuhiro Tamura : Petit-High Pressure Carbon Dioxide stress increases synthesis of S-Adenosylmethionine and phosphatidylcholine in yeast Saccharomyces cerevisiae, Biophysical Chemistry, Vol.231, 79-86, 2017. (Summary) Petit-High Pressure Carbon Dioxide (p-HPCD) is a promising nonthermal technology for foods pasteurization.Cluster analysis of gene expression profiles of Saccharomyces cerevisiae exposed to various stresses exhibitedthat gene expression profile for p-HPCD stress (0.5 MPa, 25 °C) was grouped into a cluster including profilesfor Sodium Dodecyl Sulfate and Roundup herbicide. Both are detergents that can disorder membrane structurallyand functionally, which suggests that cell membrane may be a target of p-HPCD stress to cause cell growth inhibition. Through metabolomic analysis, amount of S-Adenosylmethionine (AdoMet) that is used as methyl donorto participate in phosphatidylcholine synthesis via phosphatidylethanolamine (PE) methylation pathway, wasincreased after p-HPCD treatment for 2 h. The key gene OPI3 encoding phospholipid methyltransferase that catalyzes the last two steps in PE methylation pathway was confirmed significantly induced by RT-PCR. Transcriptional expression of genes (MET13, MET16, MET10, MET17, MET6 and SAM2) related to AdoMet biosynthesis wasalso significantly induced. Choline as the PC precursor and ethanolamine as PE precursor in Kennedy pathwaywere also found increased under p-HPCD condition.We also found that amounts of most of amino acids involvingprotein synthesis were found decreased after p-HPCD treatment for 2 h. Moreover, morphological changes on cellsurface were observed by scanning electron microscope. In conclusion, the effects of p-HPCD stress on cellmembrane appear to be a very likely cause of yeast growth inhibition and the enhancement of PC synthesis couldcontribute to maintain optimum structure and functions of cell membrane and improve cell resistance toinactivation. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bpc.2017.03.003 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2017.03.003 (DOI: 10.1016/j.bpc.2017.03.003)
14.	Ryo Suzuki, Haruhiko Koizumi, Kenichi Kojima, Seijiro Fukuyama, Yasutomo Arai, Katsuo Tsukamoto, Yoshihisa Suzuki, Masaru Tachibana and Satoshi Uda : Characterization of grown-in dislocations in high-quality glucose isomerase crystals by synchrotron monochromatic-beam X-ray topography, Journal of Crystal Growth, Vol.468, 299-304, 2017. (Summary) High quality glucose isomerase (GI) single crystals are grown by using chemical cross-linked seed crystals. The crystal structure is an orthorhombic system in which the molecular arrangement is close to a body-centered cubic (bcc) one. The crystal defects, especially dislocations, in GI crystals are experimentally characterized by synchrotron monochromatic-beam X-ray topography. Two straight dislocations are clearly observed, which originate from the interface between the cross-linked seed crystal and the grown crystal. From the invisibility criterion of the dislocation images, it is experimentally identified that they are close to be of pure edge character with the Burgers vector of [1 1 1] which is typical one in bcc metal crystals. Moreover, bead-like contrasts along the dislocation images and the equal-thickness fringes, related to Pendellösung fringes, at crystal edges are clearly observed, which have never been observed in other protein crystals so far. These contrasts can attributed to the dynamical diffraction effect which has been often observed in high-quality crystals such as Si. Thus it seems that the perfection of GI crystals shown in this paper is extremely high compared with other protein crystals reported so far. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2016.10.047 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2016.10.047 (DOI: 10.1016/j.jcrysgro.2016.10.047)
15.	Liyuan Niu, Kazuki Nomura, Hitoshi Iwahashi, Hiroyuki Matsuoka, Satoshi Kawachi, Yoshihisa Suzuki and Katsuhiro Tamura : Urea cycle is enhanced by petit-high pressure carbon dioxide stress in yeast Saccharomyces cerevisiae, High Pressure Research, Vol.37, No.1, 1-8, 2016. (Summary) It has been demonstrated that pasteurized effect on microorganisms of petit-high pressure carbon dioxide (p-HPCD) with long time treatment is similar to the effect of HPCD with short time treatment. The petit-high pressure refers to a pressure of 1.513atm (standard atmosphere). After 0.5MPa of CO2 at 25°C for 2h treatment, specific growth rate of yeast cells in the logarithm phase was decreased by 50% approximately. Under this condition, our study analyzed transcriptional responses of Saccharomyces cerevisiae through the functional genomic approach. Transcription of 837 open reading frames (ORFs) was altered relative to cells without treatment and 476 ORFs were induced after p-HPCD treatment. These selected genes were then categorized by function of gene product using the Munich Information Centre for Protein Sequences database. Genes involved in metabolism of the urea cycle were found to be significantly induced. This enhanced metabolic process could help to remove redundant HCO3 in cellular interior, thereby decrease the production of CO23. Liberation of H+ ion could be decreased along with the inhibition of HCO3 dissociation. (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2016.1261404 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2016.1261404 (DOI: 10.1080/08957959.2016.1261404)
16.	Haruhiko Koizumi, Ryo Suzuki, Masaru Tachibana, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama, Yoshihisa Suzuki, Satoshi Uda and Kenichi Kojima : Importance of Determination of Crystal Quality in Protein Crystals when Performing High-Resolution Structural Analysis, Crystal Growth & Design, Vol.16, 4906-4909, 2016. (Summary) We show that the extinction effect explained by the dynamical theory of diffraction must be considered even in the case of protein crystals. Equal-thickness fringes, which are attributed to the Pendello sung effect, were clearly observed in the region of a tapered glucose isomerase crystal with wedge-like edges using X-ray topography carried out with a beam of monochromatic synchrotron radiation. This indicates that the perfection of this glucose isomerase crystal is high enough to produce this dynamical theory-related effect: this surely leads to difficulty in the collection of accurate integrated intensities of diffraction spots for Xray structural analysis. Therefore, it is important to determine whether the crystal quality of a protein crystal under analysis is adequate to obtain accurate three-dimensional structures of protein molecules for X-ray structural analyses. We show that X-ray diffraction rocking-curve measurements can provide clues for this determination. (Link to Publication Site) ● Publication site (DOI): 10.1021/acs.cgd.6b00457 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/acs.cgd.6b00457 (DOI: 10.1021/acs.cgd.6b00457)
17.	Yoshihisa Suzuki, Yoshiaki Hattori, Jun Nozawa, Satoshi Uda, Akiko Toyotama and Junpei Yamanaka : Adsorption, Desorption, Surface Diffusion, Lattice Defect Formation, and Kink Incorporation Processes of Particles on Growth Interfaces of Colloidal Crystals with Attractive Interactions, Crystals, Vol.6, No.7, 80-1-80-11, 2016. (Summary) Good model systems are required in order to understand crystal growth processes because, in many cases, precise incorporation processes of atoms or molecules cannot be visualized easily at the atomic or molecular level. Using a transmission-type optical microscope, we have successfully observed in situ adsorption, desorption, surface diffusion, lattice defect formation, and kink incorporation of particles on growth interfaces of colloidal crystals of polystyrene particles in aqueous sodium polyacrylate solutions. Precise surface transportation and kink incorporation processes of the particles into the colloidal crystals with attractive interactions were observed in situ at the particle level. In particular, contrary to the conventional expectations, the diffusion of particles along steps around a two-dimensional island of the growth interface was not the main route for kink incorporation. This is probably due to the number of bonds between adsorbed particles and particles in a crystal; the number exceeds the limit at which a particle easily exchanges its position to the adjacent one along the step. We also found novel desorption processes of particles from steps to terraces, attributing them to the assistance of attractive forces from additionally adsorbing particles to the particles on the steps. (Keyword) colloidal crystal / polystyrene particle / sodium polyacrylate / attractive interaction / surface diffusion / incorporation process (Tokushima University Institutional Repository) ● Metadata: 115077 (Link to Publication Site) ● Publication site (DOI): 10.3390/cryst6070080 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84983304245 (Tokushima University Institutional Repository: 115077, DOI: 10.3390/cryst6070080, Elsevier: Scopus)
18.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, Hitoshi Miura and Takahisa Fujiwara : First Direct Observation of Impurity Effects on the Growth Rate of Tetragonal Lysozyme Crystals under Microgravity as Measured by Interferometry, Crystal Growth & Design, Vol.15, 4787-4794, 2015. (Summary) The suppression of impurity effects on protein crystallization under microgravity would be related to the origin of the higher X-ray resolution limits observed for space-grown crystals, and the mechanism that the impurity effects are suppressed would be useful for producing high-quality protein crystals on the ground. The normal growth rates R and apparent step velocities (lateral growth rates of a spiral hillock) V of tetragonal hen egg-white lysozyme (HEWL) crystals were for the first time measured by Michelson interferometry in the international space station (as part of the NanoStep project) using commercialized HEWL samples containing 1.5% impurities. A significant increase in V under microgravity was confirmed compared to step velocities Vstep on ground, while a decrease in R was also confirmed compared to that in the purified solution under microgravity; therefore, even under microgravity, the purity level influences crystal growth processes as expected. More precisely, because of exact measurement of growth rates, kinetic analyses of R were conducted as a function of supersaturation, σ (σ ≡ ln(C/Ce), where C is the concentration of lysozyme, and Ce is the solubility), using a spiral growth model and a two-dimensional (2D) nucleation growth model. For both models over a wide range of σ, R in the impure solution was significantly lower than that in the purified solution. Thus, the final quality of the crystals would depend on the purity of the solutions even in microgravity environments. The degree of the suppression of impurity effects was also evaluated using the difference between apparent step velocities Vp and Vi, where Vp and Vi are the apparent step velocity in the purified and impure solution, respectively. The difference between Vp and Vi became significantly smaller than the difference in step velocities on the ground, Vstep,p and Vstep,i, where Vstep,p and Vstep,i are the step velocities in the pure and impure solution, respectively. Thus, the suppression of impurity effects under microgravity was confirmed for the first time. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1021/acs.cgd.5b00456 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84943570367 (DOI: 10.1021/acs.cgd.5b00456, Elsevier: Scopus)
19.	Takahisa Fujiwara, Yoshihisa Suzuki, Izumi Yoshizaki, Katsuo Tsukamoto, Kenta Murayama, Seijiro Fukuyama, Kouhei Hosokawa, Kentaro Oshi, Daisuke Ito, Tomoya Yamazaki, Masaru Tachibana and Hitoshi Miura : Correction of the equilibrium temperature caused by slight evaporation of water in protein crystal growth cells during long-term space experiments at International Space Station, The Review of Scientific Instruments, Vol.86, 083704-1-083704-6, 2015. (Summary) The normal growth rates of the {110} faces of tetragonal hen egg white lysozyme (HEWL) crystals, R, was measured as a function of the supersaturation σ parameter using a reflection type interferometer under μG at the International Space Station (ISS, NanoStep Project). Since water slightly evaporated from in situ observation cells during a long term space station experiment for several months, equilibrium temperature Te changed, and the actual σ, however, significantly increased mainly due to the increase in salt concentration Cs. To correct σ, the actual Cs and protein concentration Cp, which correctly represent the measured Te value in space, were first calculated. Second, a new solubility curve with the corrected Cs was plotted. Finally, the revised σ was obtained from the new solubility curve. This correction method successfully revealed that the 2.8% water was evaporated from the solution, leading to 2.8% increase in the Cs and Cp of the solution. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1063/1.4928491 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84939864910 (DOI: 10.1063/1.4928491, Elsevier: Scopus)
20.	Satoshi Kawachi, Yoshihisa Suzuki, Yasuhiro Uosaki and Katsuhiro Tamura : Microbial Reduction and Quality Changes in Powdered White and Black Pepper by Treatment with Compressed Oxygen or Carbon Dioxide Gas, Food Science and Technology Research, Vol.21, No.1, 51-57, 2015. (Summary) Powdered white and black peppers were treated with high-pressure oxygen (10 MPa) or carbon dioxide (5 MPa)at 70 _ 100 to decrease the microbial populations. The degree of microbial reduction in the pepper after treatment with high-pressure gases was dependent on the temperature and duration of treatment. Mesophilic aerobic bacterial counts of less than 103 CFU/g in white and black pepper were achieved with gas pressurization at 100 for 10 and 40 min, respectively. The reduction in the microbial population of the two types of pepper was attributed to the microbicidal effects of high-pressure gas and heat treatment. The piperine content of the pepper treated with gases decreased by approximately 10%. However, treated pepper also showed lower L* values, indicating a darker color, and qualitative alterations in volatile compounds. (Keyword) pepper / high pressure / carbon dioxide / microbial decontamination (Link to Publication Site) ● Publication site (DOI): 10.3136/fstr.21.51 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3136/fstr.21.51 (DOI: 10.3136/fstr.21.51)
21.	Atsushi Mori and Yoshihisa Suzuki : Identification of triangular-shaped defects often appeared in hard-sphere crystals grown on a square pattern under gravity by Monte Carlo simulations, Physica B : Condensed Matter, Vol.452, No.1, 58-65, 2014. (Summary) In thispaper,we have successfully identified the triangular-shaped defect structures withstackingfault tetrahedra. These structure often appeared in hard-sphere (HS) crystals grown on a square patter nunder gravity. Wehave, so far, performed Monte Carlosimulations of the HS crystals under gravity. Single stacking faults as observed previously in the HScrystals grown on a flat wall were not seen in the case of square template. Instead, defect structures with triangular appearance in xz- and yz- projections were appreciable. We have identified them by looking layer by layer. Those structures are surrounded by stacking faults along face-centered cubic (fcc) {111}. Also, we see isolated vacanciesandvacancy interstitial pairs, and we have found octahedral structures surrounded by stacking faults along fcc {111}. (Keyword) colloidal crystal / Colloidal epitexy / Sedimentation / Hard spheres / Defect structures / Monte Carlo simualations (Link to Publication Site) ● Publication site (DOI): 10.1016/j.physb.2014.07.012 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.physb.2014.07.012 (DOI: 10.1016/j.physb.2014.07.012)
22.	Mamoru Fujine, Masahide Sato, Tetsuya Toyooka, Hiroyasu Katsuno, Yoshihisa Suzuki and Tsutomu Sawada : Crystallization of Brownian particles in thin systems constrained by walls, Physical Review E, Statistical, Nonlinear, and Soft Matter Physics, Vol.90, 032404-1-.032404-7, 2014. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1103/PhysRevE.90.032404 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84907267716 (DOI: 10.1103/PhysRevE.90.032404, Elsevier: Scopus)
23.	Haruhiko Koizumi, Masaru Tachibana, Izumi Yoshizaki, Seijiro Fukuyama, Katsuo Tsukamoto, Yoshihisa Suzuki, Satoshi Uda and Kenichi Kojima : Dislocations in High-quality Glucose Isomerase Crystals Grown from Seed Crystals, Crystal Growth & Design, Vol.14, No.10, 5111-.5116, 2014. (Link to Publication Site) ● Publication site (DOI): 10.1021/cg500731v (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84907524327 (DOI: 10.1021/cg500731v, Elsevier: Scopus)
24.	Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : Ordering of Brownian Particles from Walls Due to an External Force, Journal of Crystal Growth, Vol.401, 87-92, 2014. (Summary) Keeping the formation of colloidal crystal under a centrifugal force in mind, we study the ordering of Brownian particles under a uniform external force. Owing to the external force, the particles move in the direction of the external force. Near walls, the density of particles increases and the ordering of particles occurs on the walls at first. Then, the ordering in bulk proceeds gradually. Domains with the face-centered cubic and hexagonal close-packed structures are created in bulk. By controlling the direction and strength of external force, the ratio of the two types of structures changes. © 2014 Elsevier B.V. All rights reserved. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2014.01.074 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1574231877421128064 ● Summary page in Scopus @ Elsevier: 2-s2.0-84906966701 (DOI: 10.1016/j.jcrysgro.2014.01.074, CiNii: 1574231877421128064, Elsevier: Scopus)
25.	Yoshihisa Suzuki, Atsushi Mori, Masahide Sato, Hiroyasu Katsuno and Tsutomu Sawada : Colloidal crystallization on tilted substrates under gravitational fields, Journal of Crystal Growth, Vol.401, No.1, 905-909, 2014. (Summary) We show usefulness of a side wall of a tilted growth container during continuous growth of colloidal crystals under gravitational fields. The side wall works as an effective substrate which enables both enlargement of grains of colloidal crystals and control of crystallographic orientations of the grains. We successfully fabricated large grains of polystyrene face-centered cubic colloidal crystals which align with their {111} planes parallel to a side wall by tilting a growth container during centrifugation. Although, without tilting a growth container, widths and crystallographic orientations of grains remained unchanged once the widths become asymptotic values, we could increase both widths of grains and population of the grains which aligned with their {111} planes parallel to a side wall with terminating grains of the other orientations by tilting the container. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2013.11.101 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84906965906 (DOI: 10.1016/j.jcrysgro.2013.11.101, Elsevier: Scopus)
26.	Mamoru Fujine, Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : Effect of container shape and walls on solidification of Brownian particles in a narrow system, Physical Review E, Statistical, Nonlinear, and Soft Matter Physics, Vol.89, 042401, 2014. (Summary) We carry out Brownian dynamics simulations and study the ordering of particles under a uniform external force in a narrow system. In our previous studies [M. Sato et al. Phys. Rev. E 87, 032403 (2013); J. Phys. Soc. Jpn. 82, 084804 (2013)], we showed that the ordering of particles depends on the direction of the external force. In the studies, however, the system size and the number of particles are small, so that the behaviors we observed corresponds to the motions in the initial stage of crystallization. In this paper, using a longer container and more particles, we investigate how solidification in a narrow system proceeds. We also study the effect of the shape of simulation box on the ordering of particles. When we use a rhomboid as a simulation box, the ratio of the particles with the face-centered cubic structure to those with the hexagonal close-packed structure is larger than that in a cuboid system. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1103/PhysRevE.89.042401 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24827254 ● Summary page in Scopus @ Elsevier: 2-s2.0-84899741152 (DOI: 10.1103/PhysRevE.89.042401, PubMed: 24827254, Elsevier: Scopus)
27.	Izumi Yoshizaki, Katsuo Tsukamoto, Tomoya Yamazaki, Kenta Murayama, Kentaro Oshi, Seijiro Fukuyama, Taro Shimaoka, Yoshihisa Suzuki and Masaru Tachibana : Growth rate measurements of lysozyme crystals under microgravity conditions by laser interferometry, The Review of Scientific Instruments, Vol.84, 103707-1-103707-8, 2013. (Summary) The growth rate vs. supersaturation of a lysozyme crystal was successfully measured in situ together with the crystal surface observation and the concentration measurements onboard the International Space Station. A Michelson-type interferometer and a Mach-Zehnder interferometer were, respectively, employed for real-time growth rate measurements and concentration field measurements. The hardware development, sample preparation, operation, and analysis methods are described. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1063/1.4826090 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84887327809 (DOI: 10.1063/1.4826090, Elsevier: Scopus)
28.	Yoshihisa Suzuki, Gen Sazaki, Kaori Hashimoto, Takahisa Fujiwara and Yoshinori Furukawa : Colloidal crystallization utilizing interfaces of unidirectionally growing ice crystals, Journal of Crystal Growth, Vol.383, 67-71, 2013. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2013.08.026 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2013.08.026 (DOI: 10.1016/j.jcrysgro.2013.08.026)
29.	Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : Crystallization of Brownian Particles from Walls Induced by a Uniform External Force, Journal of the Physical Society of Japan, Vol.82, No.8, 084804-1-084804-8, 2013. (Summary) Keeping the formation of colloidal crystal under a centrifugal force in mind, we study the ordering of Brownian particles induced by a uniform external force. When the uniform external force is added, the particles move in the direction of the external force and the density of particles near walls becomes high. The ordering of particles starts on the walls, and successive ordering in bulk occurs near the walls. In bulk, both domains of the face-centered cubic structure and hexagonal close-packed structure appear. By controlling the direction and the strength of the external force, the number of ordered particles and distribution of cluster size are changed. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.7566/JPSJ.82.084804 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84880884785 (DOI: 10.7566/JPSJ.82.084804, Elsevier: Scopus)
30.	Atsushi Mori and Yoshihisa Suzuki : Vanishing linear term in chemical potential difference in volume term of work of critical nucleus formation for phase transition without volume change, Journal of Crystal Growth, Vol.375, No.1, 16-19, 2013. (Summary) A question is given on the form n(μ_β-μ_α) for the volume term of work of formation of critical nucleus. Here, n is the number of molecule undergone the phase transition, μ denotes the chemical potential, α and β represent the parent and nucleating phases, respectively. In this paper we concentrate phase transition without volume change. We have calculated the volume term in terms of the chemical potential difference μ_{re}-μ_{eq} for this case. Here, μ_{re}$ is the chemical potential of the reservoir and μ_{eq} that at the phase transition. We have W_{vol} = -[(κ_β-κ_α)/2v_{eq}^2] (μ_{re}-μ_{eq})^2 V_βwith κ denoting the isothermal compressibility, v_{eq} being the molecular volume at the phase transition, V_β the volume of the nucleus. (Keyword) nucleaton / cluster formation work / critical nucleus / chemical potential difference / phase transition without volume change (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2013.04.005 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2013.04.005 (DOI: 10.1016/j.jcrysgro.2013.04.005)
31.	Atsushi Mori and Yoshihisa Suzuki : Grand potential formalism of interfacial thermodynamics for critical nucleus, Natural Science, Vol.5, No.5, 631-639, 2013. (Summary) In the nucleation theory, the work of formation of a nucleus is often denoted by W=ΔG. This convention misleads that the nucleation should be considered in the isothermal-isobaric system. However, the pressure in the system with a nucleus is no longer uniform due to Laplace's equation.Instead, the chemical potential is uniform throughout the system for the critical nucleus. Therefore, one can consider the nucleation in the grand ensemble properly. Accordingly, W is found to be the grand potential difference and the interfacial tension is also turned to be an interfacial excess grand potential. This treatment is not entirely new; however, to explicitly treat in the grand potential formalism is for the first time. We have successfully given an overwhelmingly clear description. (Keyword) Gibbs interfacial thermodynamics / grad potential / interfacial tension / work of nucleus formation (Link to Publication Site) ● Publication site (DOI): 10.4236/ns.2013.55078 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.4236/ns.2013.55078 (DOI: 10.4236/ns.2013.55078)
32.	Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : Formation of a crystal of Brownian particles under a uniform external force, Physical Review E, Statistical, Nonlinear, and Soft Matter Physics, Vol.87, No.3, 032403, 2013. (Summary) To keep the formation of colloidal crystal under a centrifugation in mind, we study ordering of Brownian particles under a uniform external force. When the force is added to Brownian particles distributing uniformly in the system, the particles drift and the density of particles near walls increases. Ordering of particles on the walls occurs at first and ordering in bulk occurs in succession. In bulk, both the clusters with face-centered cubic structure and those with the hexagonal close-packed structure appear. The distribution of cluster sizes changes with the direction of external force. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1103/PhysRevE.87.032403 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1050845760916926976 ● Search Scopus @ Elsevier (DOI): 10.1103/PhysRevE.87.032403 (DOI: 10.1103/PhysRevE.87.032403, CiNii: 1050845760916926976)
33.	Yoshihisa Suzuki, Jin Endoh, Atsushi Mori, Tomoki Yabutani and Katsuhiro Tamura : Gravitational Annealing of Colloidal Crystals, Defect and Diffusion Forum, Vol.323-325, 555-558, 2012. (Summary) A silica colloidal crystal obtained by centrifugation at 9 G for 2 days in water was annealed by additional stronger centrifugation at 50 G for 5 days. The number of the striations observed in the colloidal crystal under crossed polarized light decreased at some parts in a growth container after the additional centrifugation, while the number also increased at the other parts. The decrease probably shows the shrinkage of the stacking disorders under high gravity, while the increase probably shows the production of new stacking disorders. (Keyword) crystal growth / Colloidal crystal / Gravitational annealing / Centrifugation / Lattice disorder / Stacking disorder (Link to Publication Site) ● Publication site (DOI): 10.4028/www.scientific.net/DDF.323-325.555 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.4028/www.scientific.net/DDF.323-325.555 (DOI: 10.4028/www.scientific.net/DDF.323-325.555)
34.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Monte Carlo Simulation of Defects in Hard-Sphere Crystal Grown on a Square Pattern, World Journal of Engineering, Vol.9, No.1, 37-44, 2012. (Summary) Monte Carlo simulations of hard-sphere (HS) crystal gown on a square patterned wall under gravity have been performed. While previous simulations were performed with step-wise controlled gravity, in the present simulations the gravity which is constant from the first has been applied. In the case in which a flat wall is used as the bottom wall, if a large gravity is suddenly applied, the system does polycrystallize. On the other hand, in the present simulations, despite the sudden application of gravity, the system has not polycrystallize. Crystalline nucleation on the square pattern and successive crystal growth upward are suggested to overcome the homogeneous nucleation inside and result in. Defect disappearance, which has been essentially the same as that for the case with step-wise controlled gravity, has also observed for the present case. The characteristic of the square patterned bottom wall simulation with a large horizontal system size has been existence of triangular defects suggesting stacking tetrahedra. (Keyword) hard spheres / colloidal crystal / gravity / sedimentation / colloidal epitaxy / Shockley partial dislocation / stacking fault tetrahedra (Link to Publication Site) ● Publication site (DOI): 10.1260/1708-5284.9.1.37 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1260/1708-5284.9.1.37 (DOI: 10.1260/1708-5284.9.1.37)
35.	Takahisa Fujiwara, Yoshihisa Suzuki and Katsuhiro Tamura : Solubility measurements by in situ observation of the apex region formed by the (110), (1-10) and (101) faces of tetragonal lysozyme crystals, Journal of Crystal Growth, Vol.334, No.1, 134-137, 2011. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2011.08.007 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2011.08.007 (DOI: 10.1016/j.jcrysgro.2011.08.007)
36.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : Effects of high pressure on the step velocity on the {110} faces of tetragonal lysozyme crystals, World Journal of Engineering, Vol.8, No.4, 307-312, 2011. (Link to Publication Site) ● Publication site (DOI): 10.1260/1708-5284.8.4.307 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1260/1708-5284.8.4.307 (DOI: 10.1260/1708-5284.8.4.307)
37.	Satoshi Kawachi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of gas pressurization with ethylene on the ultrastructure of the yeast Saccharomyces cerevisiae, Bioscience, Biotechnology, and Biochemistry, Vol.75, No.4, 790-792, 2011. (Summary) We investigated ultrastructural changes in the yeast Saccharomyces cerevisiae when exposed to compressed ethylene gas. Transmission electron microscopy (TEM) revealed that intracellular organelles in yeast cells treated with compressed ethylene at up to 0.640 MPa (6.4 atm), especially the nuclear and plasma membranes, were seriously damaged. (Keyword) Air Pollutants / Ethylenes / Gases / Pressure / Saccharomyces cerevisiae (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.100871 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21512223 ● Search Scopus @ Elsevier (PMID): 21512223 ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.100871 (DOI: 10.1271/bbb.100871, PubMed: 21512223)
38.	Yoshihisa Suzuki, Atsushi Mori, Takahisa Fujiwara and Katsuhiro Tamura : Precise characterization of grain structures, stacking disorders and lattice disorders of a close-packed colloidal crystal, Journal of Crystal Growth, Vol.322, No.1, 109-113, 2011. (Summary) The perpendicular fracture surface of a dried colloidal crystal with pillar-like grains, obtained by centrifugation of a dispersion of polystyrene particles, was observed using a scanning electron microscope. Many grain boundaries on the fracture surface were observed at the particle level. Most of the particles on the surface showed a face-centered cubic (FCC) array. Although some grains were single FCC ones, other FCC grains contained some stacking disorders. Most of the surface was covered with such grains, and the grain boundaries formed a mosaic-like pattern. From these results, we confirmed that the colloidal crystals obtained by centrifugation formed a bundle structure of pillar-like FCC grains. A fracture surface adjacent to the side wall of the growth cell was also observed. The surface was composed of several layers. In the uppermost layer closest to the wall, numerous point defects and mismatches of triangular lattices between the neighboring two-dimensional islands were observed. These mismatches and point defects probably generated several lattice defects in the crystal. Similar generation of lattice defects probably occurred at the bottom of the container or the growth front of the crystals. Screw dislocations were also found in the layers, although they were not observed frequently. From these results, it was concluded that two-dimensional nucleation growth and spiral growth probably occurred on the crystal-dispersion interfaces of colloidal crystals as well as on the surface of atomic crystals. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2011.03.027 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2011.03.027 (DOI: 10.1016/j.jcrysgro.2011.03.027)
39.	Yoshihisa Suzuki, Emi Konda, Hironori Hondoh and Katsuhiro Tamura : Effects of temperature, pressure, and pH on the solubility of triclinic lysozyme crystals, Journal of Crystal Growth, Vol.318, No.1, 1085-1088, 2011. (Summary) We measured the temperature dependence of the solubility, Ce, of triclinic lysozyme crystals on temperature at two pressures (0.1 and 100 MPa) and five pHs (4.3, 4.4, 4.5, 4.6, 4.7) by in situ observation of the morphological changes in the crystals. The solubility increased with increasing temperature and pressure. The enthalpies of dissolution (ΔH) decreased with increasing pressure when we used the same 50 mM sodium acetate buffer solution (pH = 4.5 at 0.1 MPa). The effects of pH on solubility were somehow complicated. Although the solubilities at pH 4.7 did not differ from those at pH 4.5, those at pH 4.3 were significantly larger than those at pH 4.5. Since the pH of an acetate buffer is known to decrease from 4.71 to 4.52 at 98.1 MPa and 25 ºC [El'yanov et al. Aust. J. Chem. 28 (1975) 933], the pH of the acetate buffer (pH 4.5 at 0.1 MPa) decreased to pH ~ 4.3 as pressure increased to 100 MPa. Thus, from the viewpoint of ``constant pH'', ΔH increased with increasing pressure, since ΔH at pH 4.3 and 0.1 MPa was smaller than that at 100 MPa. This inconsistency is probably due to the change in the hydration state in the crystal and solution with decreasing pH (from 4.5 to 4.3). (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2010.11.107 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2010.11.107 (DOI: 10.1016/j.jcrysgro.2010.11.107)
40.	Yoshihisa Suzuki, Tsutomu Sawada and Katsuhiro Tamura : Colloidal crystallization by a centrifugation method, Journal of Crystal Growth, Vol.318, No.1, 780-783, 2011. (Summary) Colloidal crystals of high particle concentration were smoothly and continuously fabricated by simple centrifugation of a dispersion of polystyrene particles in water. The larger the applied centrifugal acceleration at the bottom of the growing crystals (i.e., the interior wall of the container, the higher the growth rates became. The average grain sizes in the crystals increased with decreasing , and below a critical value of , we obtained relatively large columnar-shaped grains. The average volume fraction of the obtained colloidal crystals ave. increased with an increase in the dispersion's KCl concentration CKCl: at = 82 G, ave. = 0.57 and 0.61 at CKCl = 2 × 10-3 and 8 × 10-3 molkg-1 water, respectively. The grain size also increased with an increase in CKCl. The maximum grain size in this study was greater than 0.3 mm in width and 8 mm in length. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2010.11.036 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcrysgro.2010.11.036 (DOI: 10.1016/j.jcrysgro.2010.11.036)
41.	Yoshihisa Suzuki, Takeshi Maruoka and Katsuhiro Tamura : Activation volume of crystallization and effects of pressure on the three-dimensional nucleation rate of glucose isomerase, High Pressure Research, Vol.30, No.4, 483-489, 2010. (Summary) An activation volume, DV , of the solute incorporation process at the kink site of a glucose isomerase (GI) crystal was estimated using the pressure dependency of the step kinetic coefficient, betastep, and the three-dimensional nucleation rates J of GI crystals were analyzed using classical nucleation theory. DV took a negative value. The sticking parameter, s, for the addition of a GI tetramer to a critical nucleus drastically increased with increasing pressure. This was probably due to the decrease in the activation energy needed for the addition of the GI tetramer. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1080/08957959.2010.526941 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2010.526941 (DOI: 10.1080/08957959.2010.526941)
42.	Satoshi Kawachi, Yoshio Hara, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of compressed hydrocarbon gases on the growth activity of Saccharomyces cerevisiae, Bioscience, Biotechnology, and Biochemistry, Vol.74, No.10, 1991-1996, 2010. (Summary) The inhibitory action of compressed hydrocarbon gases on the growth of the yeast Saccharomyces cerevisiae was investigated quantitatively by microcalorimetry. Both the 50% inhibitory pressure (IP(50)) and the minimum inhibitory pressure (MIP), which are regarded as indices of the toxicity of hydrocarbon gases, were determined from growth thermograms. Based on these values, the inhibitory potency of the hydrocarbon gases increased in the order methane < ethane < propane < i-butane < n-butane. The toxicity of these hydrocarbon gases correlated to their hydrophobicity, suggesting that hydrocarbon gases interact with some hydrophobic regions of the cell membrane. In support of this, we found that UV absorbing materials at 260 nm were released from yeast cells exposed to compressed hydrocarbon gases. Additionally, scanning electron microscopy indicated that morphological changes occurred in these cells. (Keyword) Absorption / Calorimetry / Gases / Hydrocarbons / Microscopy, Electron, Scanning / Pressure / Saccharomyces cerevisiae / Thermography / Ultraviolet Rays (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.100156 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20944432 ● Search Scopus @ Elsevier (PMID): 20944432 ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.100156 (DOI: 10.1271/bbb.100156, PubMed: 20944432)
43.	Yoshihisa Suzuki, Masayuki Tsukamoto, Haruhiko Sakuraba, Masamitsu Matsumoto, Makoto Nagasawa and Katsuhiro Tamura : Design of a stand-alone-type Beryllium vessel for high-pressure protein crystallography, The Review of Scientific Instruments, Vol.81, No.8, 084302-1-084302-3, 2010. (Summary) A standalone-type beryllium (Be) high-pressure crystallography vessel has been developed. Using a coupler-joint unit and a pressure valve, we could keep the pressure in the vessel constant at 100+/-1 MPa for more than 24 h without connecting to a pressure-generating apparatus. Diffraction spots of a glucose isomerase (GI) crystal under 100 MPa were collected using the vessel and a rotating copper-anode in-house x-ray generator (0.8 kW). We successfully collected a 2.0 A resolution data set of a 0.5 mm size GI crystal in an aqueous solution at 100 MPa. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1063/1.3478000 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20815618 ● Search Scopus @ Elsevier (PMID): 20815618 ● Search Scopus @ Elsevier (DOI): 10.1063/1.3478000 (DOI: 10.1063/1.3478000, PubMed: 20815618)
44.	Atsushi Mori and Yoshihisa Suzuki : Interplay between elastic fields due to gravity and a partial dislocation for a hard-sphere crystal coherently grown under gravity: driving force for defect disappearance, Molecular Physics, Vol.108, No.13, 1731-1738, 2010. (Summary) We previously observed that an intrinsic staking fault shrunk through a glide of a Shockley partial dislocation terminating its lower end in a hard-sphere crystal under gravity coherently grown in <001> by Monte Carlo simulations [Mori et al., Molec. Phys. 105, 1377 (2007)]; it was an answer to a one-decade long standing question why the stacking disorder in colloidal crystals reduced under gravity [Zhu et al., Nature 387, 883 (1997)]. Here, we present an elastic energy calculation; in addition to the self-energy of the partial dislocation [Mori et al., Prog. Theor. Phys. Suppl. 178, 33 (2009)] we calculate the cross-coupling term between elastic field due to gravity and that due to a Shockley partial dislocation. The cross term is an increasing function of the linear dimension R over which the elastic field expands, showing that a driving force arises for the partial dislocation moving toward the upper boundary of a grain. (Keyword) elasticity / Shockley partial dislocation / gravity / hard-sphere crystal (Link to Publication Site) ● Publication site (DOI): 10.1080/00268976.2010.489523 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1080/00268976.2010.489523 (DOI: 10.1080/00268976.2010.489523)
45.	Satoshi Kawachi, Toshiaki Arao, Yoshihisa Suzuki, Katsuhiro Tamura and : Effects of compressed unsaturated hydrocarbon gases on yeast growth, Annals of the New York Academy of Sciences, Vol.1189, 121-126, 2010. (Summary) The effect of compressed unsaturated hydrocarbon gases on the growth of the yeast Saccharomyces cerevisiae was investigated by microcalorimetry. The growth thermograms showed that unsaturated hydrocarbon gases inhibited yeast growth. As an approach to determining the comparative toxicity of unsaturated hydrocarbon gases, we determined the 50% inhibitory pressure (IP(50)) and the minimum inhibitory pressure (MIP). On the basis of the IP(50) and MIP values, the inhibitory potency of the gases increased in the order ethylene < propylene < 1-butene. Additionally, scanning electron microscopy showed that cells treated with unsaturated hydrocarbon gases were damaged, including invagination of the cell surface. (Keyword) bioassay (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1749-6632.2009.05206.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233377 ● Search Scopus @ Elsevier (PMID): 20233377 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05206.x (DOI: 10.1111/j.1749-6632.2009.05206.x, PubMed: 20233377)
46.	Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima and Katsuhiro Tamura : First Direct Observation of Elementary Steps on the Surfaces of Glucose Isomerase Crystals under High Pressure, Crystal Growth & Design, Vol.9, No.10, 4289-4295, 2009. (Keyword) crystal growth (Link to Publication Site) ● Publication site (DOI): 10.1021/cg800119w (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/cg800119w (DOI: 10.1021/cg800119w)
47.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Disappearance of a Stacking Fault in Hard-Sphere Crystals under Gravity, Progress of Theoretical Physics Supplement, Vol.178, 33-40, 2009. (Summary) In the first part of this paper, a review is given on the mechanism for the disappearance of an intrinsic stacking fault in a hard-sphere (HS) crystal under gravity, which we recently discovered by Monte Carlo (MC) simulations [A. Mori et al., J. Chem. Phys. 124 (2006), 17450; Mol. Phys. 105 (2007), 1377]. We have observed, in the case of fcc (001) stacking, that the intrinsic stacking fault running along an oblique direction shrunk through the gliding of a Shockley partial dislocation at the lower end of the stacking fault. In order to address the shortcomings and approximations of previous simulations, such as the use of periodic boundary condition (PBC) and the fact that the fcc (001) stacking had been realized by the stress from the small PBC box, we present an elastic strain energy calculation for an infinite system and a MC simulation result for HSs in a pyramidal pit under gravity. In particular, the geometry of the latter has already been tested experimentally [S. Matsuo et al., Appl. Phys. Lett. 82 (2003), 4283]. The advantage of using a pyramidal pit as a template as well as the feasibility of the mechanism we describe is demonstrated. (Link to Publication Site) ● Publication site (DOI): 10.1143/PTPS.178.33 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1521699230840001152 ● Summary page in Scopus @ Elsevier: 2-s2.0-68049140771 (DOI: 10.1143/PTPS.178.33, CiNii: 1521699230840001152, Elsevier: Scopus)
48.	村本桂久, Katsuhiro Tamura, Toshiaki Arao, Yoshihisa Suzuki and 岩橋均 : Development of the Food Sterilization Equipment by Oxygen-Nitrogen Gas Hybrid Pressurization System, High Pressure Bioscience and Biotechnology, Vol.2, 101-108, 2008. (Summary) Microbial spoilage, including fermentation and oxidation is particularly an important factor that influences the quality of foods. In previous studies we showed that sudachi (sour citrus fruit) juice could be sterilized with oxygen gas pressurization at 10 MPa and 50°C without loss of its flavor and color. Furthermore, we reported a method to remove the dissolved oxygen in sudachi juice with nitrogen gas pressurization. However, all these results were obtained using a small-scale batch process (500 ml or less in capacity). In this research, we designed a new commercial scale food processing apparatus for the sudachi juice based on the above findings. This device consists of two parts: a sterilization part with oxygen gas pressurization and a dissolved oxygen removal part with nitrogen gas pressurization, and it has a big advantage that a large amount of sudachi juice can be treated continuously at the flow rates up to 40 L/h. Using this apparatus, the sudachi juice was sterilized under similar operation conditions (50°C and 10 MPa for 1 minute) to those of the small-scale batch system. Although the performance of dissolved oxygen removal and the quality conservation of the juice (such as flavor and color) is under investigation now, the facility and production costs are expected to be apparently less than those of high-pressure processing (liquid pressure). Because, our treatments can be completed in a short time at relatively low pressures using “oxygen-nitrogen hybrid pressurization system”. We consider further improvements of the apparatus and hope that the practical application of the method can be realized in food industry in the near future. (Keyword) sudachi juice / sterilization / oxygen / gas pressurization / hybrid pressurization (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.2.101 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205286464384 ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.101 (DOI: 10.11229/hpbb.2.101, CiNii: 1390001205286464384)
49.	Satoshi Kawachi, 原好男, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Calorimetric Studies on the Growth Behavior of Yeast under Some Compressed Hydrocarbon Gases, High Pressure Bioscience and Biotechnology, Vol.2, 88-95, 2008. (Summary) In this study, the inhibitory effect under compressed hydrocarbon gases on yeast growth was investigated quantitatively by microcalorimetry. The growth thermograms (heat output - incubation time curve) were obtained during incubation of yeast under various compressed hydrocarbon gases. When the gas pressure increased, the curves of thermograms shifted to the right, indicating an obvious inhibitory action on the yeast growth. After quantification of growth inhibition at various pressures, we determined the 50% inhibitory pressure (IP<SUB>50</SUB>) and the minimum inhibitory pressure (MIP) values as indices which represent toxic potency of each gas. The lower the IP<SUB>50</SUB> and MIP values, the greater the growth inhibitory effects of the gas. Based on these values, the inhibitory potency of the gases increased in the order: methane < ethane < propane < <I>i</I>-butane < <I>n</I>-butane. In addition, the toxicity of some hydrocarbon gases was found to be correlated to their hydrophobicity. These results suggest that hydrocarbon gases interact with some hydrophobic region of the cell membrane, resulting in modification of membrane structure and function. However, no evidence was obtained in the yeast treated with compressed hydrocarbon gases as to the membrane damage. Therefore, we investigated the effects of hydrocarbon gases on the leakage of 260 nm absorbing material from treated cells. The release amount of 260 nm absorbing material increased with increasing pressure. This indicates that hydrocarbon gases affect the plasma membrane, particularly the nuclear membrane. Furthermore, we examined the effects of hydrocarbon gases on the morphology of the yeasts by scanning electron microscopy. Scanning electron microscopy showed that yeasts when exposed to compressed hydrocarbon gases undergo a dramatic morphological change that includes invagination of the cell surface. (Keyword) hydrocarbon gas / high pressure / bioassay / yeast / metabolic heat measurement (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.2.88 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282680263134464 ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.88 (DOI: 10.11229/hpbb.2.88, CiNii: 1390282680263134464)
50.	Atsushi Mori, Yoshihisa Suzuki and Shin-ichiro Yanagiya : Succession of stacking fault in hard-sphere crystal under gravity by Monte Carlo simulation, Fluid Phase Equilibria, Vol.257, No.2, 131-138, 2007. (Summary) We have performed Monte Carlo simulations of hard spheres under gravity. Vertical boundaries are hard walls, which are well separated with each other. On the other hand, the periodic boundary condition is imposed in the horizontal direction. While we previously reported enhancement of crystallinity as well as crystallization due to gravity, we present here the results that demonstrate the succession of a defect. In case that the crystal formed at the bottom of the system includes kinds of stacking disorders for the (001) growth, twin band structure develops as mediated by a stacking disorder succeeded in the crystal formed in the fluid region which lies on the bottom crystal. In case that the stacking structure along horizontal direction changes from the (111) stacking to the (001) stacking, twin band structure in the (001) stacking region develops as succeeded in the crystal transformed. The twin band structure also becomes large with its upward growth. (Keyword) hard-sphere suspension / gravity / sedimentaion / stacking disorder / partial dislocation / Monte Carlo simulation / twin band structur (Link to Publication Site) ● Publication site (DOI): 10.1016/j.fluid.2007.01.012 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-34250782690 (DOI: 10.1016/j.fluid.2007.01.012, Elsevier: Scopus)
51.	Satoshi Kawachi, Yoshio Hara, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of Some Compresed Hydrocarbon Gases on Yeast Growth, High Pressure Bioscience and Biotechnology, Vol.1, 139-145, 2007. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.1.139 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.1.139 (DOI: 10.11229/hpbb.1.139)
52.	Tadashi Yamamoto, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of Antioxidants (α-Lipoic Acid and Vitamin E) on the Phase Transition Temperature of Bilayer Membrane of Phospholipids under Oxygen-Gas Pressure, High Pressure Bioscience and Biotechnology, Vol.1, 86-89, 2007. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.1.86 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.1.86 (DOI: 10.11229/hpbb.1.86)
53.	Yoshio Hara, Katsuhiro Tamura, Satoshi Kawachi, Toshiaki Arao and Yoshihisa Suzuki : Effects of Some Compressed Rare Gases of Yeast Growth, High Pressure Bioscience and Biotechnology, Vol.1, 146-152, 2007. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.1.146 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.1.146 (DOI: 10.11229/hpbb.1.146)
54.	Toshiaki Arao, Yoshihisa Muramoto, Takanori Taniwaki, Yoshihisa Suzuki and Katsuhiro Tamura : Sterilization of Sudachi Juice by Hybrid Gas Compression of Oxygen and Nitrogen, High Pressure Bioscience and Biotechnology, Vol.1, 264-271, 2007. (Link to Publication Site) ● Publication site (DOI): 10.11229/hpbb.1.264 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.1.264 (DOI: 10.11229/hpbb.1.264)
55.	Atsushi Mori, Yoshihisa Suzuki, Shin-ichiro Yanagiya, Tsutomu Sawada and Kensaku Ito : Shrinking stacking fault through glide of the Shockley partial dislocation in hard-sphere crystal under gravity, Molecular Physics, Vol.105, No.10, 1377-1383, 2007. (Summary) Disappearance of a stacking fault in the hard-sphere crystal under gravity, such as reported by Zhu et al. [Nature 387 (1997) 883], has successfully been demonstrated by Monte Carlo simulations. We previously found that a less ordered (or defective) crystal formed above a bottom ordered crystal under stepwise controlled gravity [Mori et al., J. Chem. Phys. 124 (2006) 174507]. A defect in the upper defective region has been identified with a stacking fault for the (001) growth. We have looked at the shrinking of a stacking fault mediated by the motion of the Shockley partial dislocation; the Shockley partial dislocation terminating the lower end of the stacking fault glides. In addition, the presence of crystal strain, which cooperates with gravity to reduce stacking faults, has been observed. (Link to Publication Site) ● Publication site (DOI): 10.1080/00268970701348725 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85017155043 (DOI: 10.1080/00268970701348725, Elsevier: Scopus)
56.	Yoshihisa Suzuki, Tsutomu Sawada, Atsushi Mori and Katsuhiro Tamura : Colloidal crystallization by centrifugation, Japanese Journal of Polymer Science and Technology, Vol.64, No.3, 161-165, 2007. (Summary) Columnar-shaped colloidal crystals (width and length of the columns -0.1-1.0 mm and 10 mm, respectively) have been grown by centrifugation of the dispersion of polystyrene particles. The crystals were re-dispersed reversibly. Close-packed colloidal crystals were obtained by drying the columnar crystals gently. The dried crystals contained no macroscopic cracks over several mm<sup>3</sup>. Some stacking disorders were observed on the cross sections of the dried crystals. The average grain size of the columnar crystals increased with decreasing centrifugal acceleration. Too fast centrifugation caused the sedimentation of randomly oriented small grains in front of the growth interfaces.<br> (Keyword) Centrifugation / centrifugation / Close-Packed Colloidal Crystal / close-packed colloidal crystal / Stacking Disorder / stacking disorder / Grain Size / grain size (Link to Publication Site) ● Publication site (DOI): 10.1295/koron.64.161 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001206523776000 ● Search Scopus @ Elsevier (DOI): 10.1295/koron.64.161 (DOI: 10.1295/koron.64.161, CiNii: 1390001206523776000)
57.	Yoshihisa Suzuki, Atsuto Arai and Katsuhiro Tamura : Solubility Measurements of Tetragonal Lysozyme Crystals, International Journal of Modern Physics B, Vol.20, No.25n27, 4117-4122, 2006. (Link to Publication Site) ● Publication site (DOI): 10.1142/S0217979206040957 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-33751261159 (DOI: 10.1142/S0217979206040957, Elsevier: Scopus)
58.	Atsushi Mori, Shin-ichiro Yanagiya, Yoshihisa Suzuki, Tsutomu Sawada and Kensaku Ito : Monte Carlo simulation of crystal-fluid coexistence states in the hard-sphere system under gravity with step-wise control, The Journal of Chemical Physics, Vol.124, No.17, 174507-1-174507-10, 2006. (Summary) Monte Carlo (MC) simulations were performed for hard spheres (with diameter sigma and mass m) placed between well-separated upper and lower hard walls. A periodic boundary condition was imposed in the horizontal direction. The system was exposed to the gravitational field with the acceleration due to gravity g. After preparing a melt as the initial state, g was increased stepwise up to mgsigma/kBT([equivalent]g)=1.5 or 2.0 with an increment Deltag=0.1; kBT is the temperature multiplied by Boltzmann's constant. We maintained g* at each value for 2.0×105 MC cycles. The transition of the system into a metastable state such as a polycrystalline state due to trapping phenomena was successfully avoided. A monotonic increase and subsequent saturation were observed for the development of the crystalline region formed at the bottom of the system. The development of this region accompanied a shrinkage of the defective (or less ordered) crystalline region that was formed between the bottom region and the fluid phase. As the development of the bottom region almost saturated, the defective region grew upward again. (Link to Publication Site) ● Publication site (DOI): 10.1063/1.2193149 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16689583 ● Search Scopus @ Elsevier (PMID): 16689583 ● Search Scopus @ Elsevier (DOI): 10.1063/1.2193149 (DOI: 10.1063/1.2193149, PubMed: 16689583)
59.	Atsushi Mori, Shin-ichiro Yanagiya, Yoshihisa Suzuki, Tsutomu Sawada and Kensaku Ito : Crystal structure of hard spheres under gravity by Monte Carlo simulation, Science and Technology of Advanced Materials, Vol.7, No.3, 296-302, 2006. (Summary) Monte Carlo simulations were performed for hard spheres (HSs) under gravity. The gravity was increased stepwise. HSs were placed between the bottom and the top hard walls. For gR0.9, we observed that a `sediment' was comprised of two crystalline and one fluid regions. Here, g is defined by g*Zmgs/kBT with m being the mass of a particle, s the HS diameter, g the acceleration due to gravity, and kBT the temperature multiplied by Boltzmann's constant. The bottom crystal was less defective or well-ordered and the crystal lay between the bottom one and the fluid phase was defective or less-ordered. In this paper, we investigate the structure of the crystals. Despite no apparent defects, the crystal has highly been distorted. That is, the fcc lattice has been contracted in the vertical direction more than in the horizontal direction. The crystal fluid coexistence condition for the bulk HS system does, in principle, not hold for the present systems at the crystal fluid interface. In addition, though the fine scale density profile exhibits a discontinuity apparently across the crystal crystal interface, the interlayer separation increases linearly with the height. (Keyword) Hard-sphere suspension / Gravity / Sedimentation / Crystal fluid coexistence / crystal structure / tooth deformation / Monte Carlo simulation (Link to Publication Site) ● Publication site (DOI): 10.1016/j.stam.2006.03.002 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.stam.2006.03.002 (DOI: 10.1016/j.stam.2006.03.002)
60.	Hiroyuki Matsuoka, Y. Suzuki, Hitoshi Iwahashi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : The biological effects of high-pressure gas on the yeast transcriptome, Brazilian Journal of Medical and Biological Research, Vol.38, No.8, 1267-1272, 2005. (Summary) The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies. (Keyword) Feasibility Studies / Gene Expression Profiling / Gene Expression Regulation, Fungal / Hydrostatic Pressure / Nitrogen / Oligonucleotide Array Sequence Analysis / RNA, Fungal / RNA, Messenger / Saccharomyces cerevisiae (Link to Publication Site) ● Publication site (DOI): 10.1590/S0100-879X2005000800016 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16082469 ● Search Scopus @ Elsevier (PMID): 16082469 ● Search Scopus @ Elsevier (DOI): 10.1590/S0100-879X2005000800016 (DOI: 10.1590/S0100-879X2005000800016, PubMed: 16082469)
61.	Shin-ichiro Yanagiya, Atsushi Mori, Yoshihisa Suzuki, Yasuo Miyoshi, Masashi Kasuga, Tsutomu Sawada, Kensaku Ito and Tetsuo Inoue : Enhancement of Crystallization of Hard Spheres by Gravity: Monte Carlo Simulation, Japanese Journal of Applied Physics, Part 1 (Regular Papers & Short Notes), Vol.44, No.7A, 5113-5116, 2005. (Summary) We present the effects of gravity on the growth of a hard-sphere (HS) crystal as determined by Monte Carlo simulations. HSs were confined between hard walls at the top (z . Lz) and the bottom (z . 0) of the system with a periodic boundary condition in the horizontal direction. After preparing a melt state as an initial state, the gravity was suddenly switched on. The values of the gravity were mg_=kBTd_ g_T . 0:1; 0:2; . . . ; 2:0, where m was the mass of a HS, g the acceleration of gravity, _ the HS diameter, and kBT the temperature multiplied by Boltzmann's constant. We observed the enhancement of crystallization due to gravity up to g_ . 0:7 and that the crystals of largest size were formed at g_ . 0:7{0:9. On the other hand, for g_ _ 1:0, the top position of the HS crystal that was grown from the bottom became lower with an increase in the gravity, where crystals with various axis directions coexisted. The polycrystallization is speculated to occur due to polynucleation. These results are qualitatively consistent with those of an experimental study of the centrifugal sedimentation of colloidal crystallization. (Keyword) hard spheres / Monte Carlo simulation / sedimentation / gravity effects / crystallization / solid-fluid interface / solid-solid interface (Link to Publication Site) ● Publication site (DOI): 10.1143/JJAP.44.5113 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001206266164608 ● Search Scopus @ Elsevier (DOI): 10.1143/JJAP.44.5113 (DOI: 10.1143/JJAP.44.5113, CiNii: 1390001206266164608)
62.	Toshiaki Arao, Yoshio Hara, Yoshihisa Suzuki and Katsuhiro Tamura : Effect of high-pressure gas on yeast growth, Bioscience, Biotechnology, and Biochemistry, Vol.69, No.7, 1365-1371, 2005. (Summary) Microcalorimetry is a useful tool for monitoring the growth behavior of microorganisms. In this study, microcalorimetry was used to investigate the effects of nitrogen, air, oxygen, nitrous oxide, argon, and krypton at high pressure on the growth of the yeast Saccharomyces cerevisiae. Growth thermograms (metabolic heat vs. incubation time) were generated to estimate metabolic activity under compressed gases and to determine the 50% inhibitory pressure (IP(50)) and minimum inhibitory pressure (MIP), which are regarded as indices of the toxicity of compressed gases. Based on MIP values, the most toxic to the least toxic gases were found to be: O(2) > N(2)O > air > Kr > N(2) > Ar. (Keyword) Air Pressure / Argon / Dose-Response Relationship, Drug / Gases / Krypton / Nitrogen / Nitrous Oxide / Oxygen / Pressure / Saccharomyces cerevisiae (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.69.1365 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16041143 ● Search Scopus @ Elsevier (PMID): 16041143 ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.69.1365 (DOI: 10.1271/bbb.69.1365, PubMed: 16041143)
63.	村本桂久, Katsuhiro Tamura, 谷脇孝典, 高井信吾 and Yoshihisa Suzuki : Removal of Dissolved Oxygen in Sudachi Juice by Nitrogen Gas Pressurization, Nippon Shokuhin Kagaku Kogaku Kaishi, Vol.52, No.4, 178-182, 2005. (Summary) 果汁中の溶存酸素は，さまざまな形で品質劣化の原因となる．本研究は，果汁を窒素ガスで加圧したのち減圧することで，窒素が溶存酸素を同伴する作用を利用して酸素脱気する技術に関するものである．20秒間隔で，加圧と除圧を7回繰り返すことにより，効率的に脱気することができた．30℃，10 MPaで処理することにより，溶存酸素量を0.02 mg/lまで下げることができた．本技術はこれまでにない新しい技術であり，特許の出願につながった． (Link to Publication Site) ● Publication site (DOI): 10.3136/nskkk.52.178 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282681384814208 ● Summary page in Scopus @ Elsevier: 2-s2.0-22244453044 (DOI: 10.3136/nskkk.52.178, CiNii: 1390282681384814208, Elsevier: Scopus)
64.	Yoshihisa Suzuki, Gen Sazaki, Matsui Takuro, Kazuo Nakajima and Katsuhiro Tamura : High Pressure Acceleration of the Growth Kinetics of Glucose Isomerase Crystals, The Journal of Physical Chemistry B, Vol.109, No.8, 3222-3226, 2005. (Summary) グルコースイソメラーゼ結晶の{101}面の成長·溶解速度を0.1, 100 MPaでその場観察により測定した．25℃において，溶解度は0.1 MPaでCe = 3.1 ± 0.9 mgml-1，100 MPaで 2.6 ± 0.5 mg ml-1 となった．同じ2.5という過飽和度で比較した結果，成長速度は100MPaにおいて0.1MPaの7.6倍になった．この結果から，加圧によって結晶の成長速度が速度論的に加速されることが初めて明らかになった．二次元核成長モデルを用いて解析した結果，加圧による成長速度の加速の原因は，主に表面自由エネルギーの減少にあることが分かった． (Keyword) Aldose-Ketose Isomerases / Chemistry, Physical / Crystallization / Kinetics / Models, Chemical / Pressure / Solubility / Streptomyces / Temperature (Link to Publication Site) ● Publication site (DOI): 10.1021/jp046419j (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16851344 ● CiNii @ National Institute of Informatics (CRID): 1571698601425958784 ● Search Scopus @ Elsevier (PMID): 16851344 ● Search Scopus @ Elsevier (DOI): 10.1021/jp046419j (DOI: 10.1021/jp046419j, PubMed: 16851344, CiNii: 1571698601425958784)
65.	村本桂久, Katsuhiro Tamura, Toshiaki Arao, 谷脇孝典 and Yoshihisa Suzuki : Sterilization of Sudachi Juice by Compressed Oxygen Gas, Nippon Shokuhin Kagaku Kogaku Kaishi, Vol.51, No.11, 604-612, 2004. (Summary) 新しい技術である酸素ガス加圧殺菌に関する研究．スダチ果汁を用い，果汁中酵母(Candida boidinii SYM-1)に対する加圧酸素ガスの毒性を調べ，殺菌条件を見積もった．30℃+5.0 MPaで15 min，40℃+10.0 MPaでは5 min，そして50℃+5.0 MPaでは1 min未満で殺菌が可能であった．50℃では，ビタミンCの減少を防ぐことができた．従来の加熱殺菌では，品質の劣化が著しかったが，本法により品質を維持しながら殺菌が可能となった．本研究は1件の特許出願につながった． (Link to Publication Site) ● Publication site (DOI): 10.3136/nskkk.51.604 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001206407323136 ● Summary page in Scopus @ Elsevier: 2-s2.0-13444254288 (DOI: 10.3136/nskkk.51.604, CiNii: 1390001206407323136, Elsevier: Scopus)
66.	Toshitaka Asai, Yoshihisa Suzuki, Gen Sazaki, Katsuhiro Tamura, Tsutomu Sawada and Kazuo Nakajima : Effects of high pressure on the solubility and growth kinetics of monoclinic lysozyme crystals, Cellular and Molecular Biology, Vol.50, No.4, 329-334, 2004. (Summary) 単斜晶系リゾチーム結晶の(010)面と(0-10)面の平均成長速度R<010>を0.1, 100MPaの圧力下でその場観察により測定した．成長速度の濃度依存性から溶解度を求めた結果，溶解度は圧力の増加に伴い増加した．0.1, 100MPaにおいて同じ過飽和度下で結晶の成長速度を比較した結果，加圧に伴い，結晶の成長速度は増加した．二次元核成長モデルを用いて解析した結果，その原因は，表面自由エネルギー，活性化エネルギー，そしてステップ上のキンク間距離の加圧に伴う減少にあることがわかった． (Keyword) Animals / Chickens / Crystallization / Kinetics / Muramidase / Pressure / Solubility / Thermodynamics (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15529742 ● Search Scopus @ Elsevier (PMID): 15529742 (PubMed: 15529742)
67.	Toshiaki Arao, Mitsuaki Asakura, Yoshihisa Suzuki, Katsuhiro Tamura, Arihide Okamoto, Hiroyuki Inubushi and Michihiro Miki : Bioassay of various pesticides by microcalorimetry measuring the metabolic heat of yeast, Environmental Sciences, Vol.11, No.6, 303-312, 2004. (Summary) Using a microcalorimeter, the heat evolved during incubation of yeast cultures at 30 degrees C was detected in the form of a growth thermogram (metabolic heat-incubation time curve). The correlation between the growth thermograms and the growth curves obtained by colony counting or from the turbidity measurements of the cultures was very good. In this study, the effects of 12 pesticides that are used widely on golf courses on the growth thermograms of yeast were investigated and the inhibitory action of the pesticides on the growth of yeast was quantitatively evaluated using a microcalorimeter (Biothermo Analyzer). The growth thermograms were applied to estimate the microbial activity of yeast in the presence of pesticide and to determine the 50% inhibitory concentration (K) and minimum inhibitory concentration (MIC), which can be regarded as good indexes of the toxic potency of pesticides. It was found that the addition of TPN, captan, thiuram and copper oxide to the yeast cultures clearly induced inhibitory action on the growth of yeast cells. On the other hand, the other 8 pesticides, iprodione, isoprothiorane, chloroneb, pencycuron, metalaxyl, toleclofos-methyl, flutolanil, and mepronil did not affect the growth over the concentration range up to 30 mg/l. (Keyword) Biological Assay / Calorimetry / Hot Temperature / Inhibitory Concentration 50 / Pesticides / Saccharomyces cerevisiae / Temperature / Time Factors / Toxicity Tests (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15750576 ● Search Scopus @ Elsevier (PMID): 15750576 (PubMed: 15750576)
68.	Yukiko Nagatoshi, Gen Sazaki, Yoshihisa Suzuki, Satoru Miyashita, Takuro Matsui, Toru Ujihara, Kozo Fujiwara, Noritaka Usami and Kazuo Nakajima : Effects of high pressure on the growth kinetics of orthorhombic lysozyme crystals, Journal of Crystal Growth, Vol.254, No.1, 188-195, 2003. (Summary) 斜方晶系リゾチーム結晶の(011)面と(0-11)面の成長過程を0.1, 100 MPaでその場観察した．0.1と100MPaで同じ過飽和度で比較したところ，両面の成長速度は加圧に伴い減少することが分かった．二次元核成長モデルで解析した結果，加圧に伴う成長速度の減少は溶質分子の結晶への取込み過程における活性化エネルギーの増加によって説明できることがわかった． (Keyword) biocrystallization / high pressure / proteins / EGG-WHITE LYSOZYME / HIGH HYDROSTATIC-PRESSURE / PROTEIN CRYSTALLIZATION / SUBTILISIN CRYSTALLIZATION / NUCLEATION KINETICS / SOLUBILITY / DEPENDENCE / MORPHOLOGY / SOLVENT (Link to Publication Site) ● Publication site (DOI): 10.1016/S0022-0248(03)01151-5 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1570291226792685184 ● Search Scopus @ Elsevier (DOI): 10.1016/S0022-0248(03)01151-5 (DOI: 10.1016/S0022-0248(03)01151-5, CiNii: 1570291226792685184)
69.	松本知浩, Katsuhiro Tamura and Yoshihisa Suzuki : Effects of dissolved gases on the phase transition of liposomal bilayer membranes, Anesthesia and Resuscitation, Vol.38, No.4, 233-236, 2002. (Summary) 2種類のリン脂質二分子膜(DMPC,DPPC)のゲル-液晶間の相転移温度(Tm)を，様々な気体と接触させた開放系で加圧して決定した．使用した気体は窒素，酸素，水素，ヘリウム，アルゴンの5種類の単一気体と4種類の混合気体である．Tmに対する圧力の影響は，加圧によりTmが直線的に上昇する気体のグループと低下するグループの2つに分かれた．加圧によりTmが低下するグループの気体は，水に対する溶解度が大きいものが多いことから，溶存気体の濃度が転移温度の低下に関与していることが推測された．特に亜酸化窒素や酸素，アルゴンなどが，Tmを押し下げる効果が大きく，これらの溶存気体のリン脂質二分子膜骨格に対する作用の大きさが示唆された． (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0036953652 (Elsevier: Scopus)
70.	Hiroshi Matsuo, Yoshihisa Suzuki and Seiji Sawamura : Solubility of α-amino acids in water under high pressure - glycine, L-alanine, L-valine, L-leucine, and L-isoleucine, Fluid Phase Equilibria, Vol.200, No.2, 227-237, 2002. (Summary) glycine, L-alanine, L-valine, L-leucine, and L-isoleucineの水に対する溶解度を298.15Kで400MPaの圧力まで測定した．その結果，アミノ酸の種類によって様々な結果が明らかになった．glycineは加圧にともない溶解度が減少したのに対し，L-alanineの溶解度は逆に増加した．L-valineとL-isoleucine は100MPa付近に溶解度の極大があり，L-leucineは200MPa付近に固相相転移が起こっていることが明らかになった． (Link to Publication Site) ● Publication site (DOI): 10.1016/S0378-3812(02)00029-8 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0036680657 (DOI: 10.1016/S0378-3812(02)00029-8, Elsevier: Scopus)
71.	Katsuhiro Tamura, 松本知浩, Toshiaki Arao and Yoshihisa Suzuki : リン脂質二分子膜に及ぼす溶存気体の影響, Anesthesia and Resuscitation, Vol.37, No.3, 77-79, 2001. (Summary) ジミリストイルフォスファチジルコリン二分子膜のゲル-液晶主転移温度(Tm)を様々な気体と接触させた解放系で加圧して決定した．使用した気体は酸素，窒素，水素，アルゴンの4種類の単一気体と空気，20.0%酸素(ヘリウムベース)，10.1%亜酸化窒素(窒素ベース)及び4.69%水素(窒素ベース)の4種類の混合気体である．Tmに対する圧力の影響は加圧によりTmが直線的に上昇する気体のグループと低下するグループの2つに分かれた．加圧によりTmが低下するグループの気体は，水に対する溶解度が大きいものが多いことから，溶存気体の濃度が転移温度の低下に関与していることが推察された．特に亜酸化窒素(笑気ガス)や酸素が，Tmを押し下げる効果が大きく，これらの溶存気体のリン脂質二分子膜骨格に対する作用の大きさが示唆された．
72.	Yoshihisa Suzuki, Hiroshi Matsuo, Yoshikata Koga, Katsuya Mukae, Tetsuya Kawakita and Seiji Sawamura : Effects of Pressure and Temperature on the Solubility of Monosodium l-Glutamate Monohydrate in Water, High Pressure Research, Vol.21, No.2, 93-104, 2001. (Summary) グルタミン酸ナトリウム一水和物の水に対する溶解度を0.10-300 MPaの圧力範囲で298.15 Kで測定した．高濃度水溶液の密度と，飽和溶液の溶解熱についても常圧下で測定した．溶解度msは加圧に伴い増加し，その圧力係数dlnms/dp=(2.0±0.1)X10^(-10) Pa^(-1)は，密度測定によって求められた部分モル体積と活量係数と結晶のモル体積から熱力学的に求められた値(2.1±0.2)X10^(-10) Pa^(-1)と一致した．溶解熱から求められた温度係数dlnms/d(1/T)は，Ogawaらによる溶解度の温度係数と良く一致した． (Link to Publication Site) ● Publication site (DOI): 10.1080/08957950108201007 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0005937994 (DOI: 10.1080/08957950108201007, Elsevier: Scopus)
73.	Katsuhiro Tamura, Toshiaki Arao, 久田和洋 and Yoshihisa Suzuki : アルコールショック酵母の増殖とストレス応答, Anesthesia and Resuscitation, Vol.36, No.4, 171-174, 2000. (Summary) 酵母に一連のアルコール(メタノール，エタノール，1-プロパノール，2-プロパノール，1-ブタノール，2-ブタノール，iso-ブタノール，tert-ブタノール)を短時間接触させることでケミカルショックを加え，誘発される二次的なストレスに対する耐性(熱耐性と圧力耐性)を見積もった．また，マイクロ熱量計を用い，培地に同様なアルコールを添加した酵母の増殖サーモグラムを描き，増殖活性に及ぼすアルコールの影響を調べた．耐性実験，サーモグラム実験共にアルコールの性質により大きく影響を受けることが分かった．アルコールの分子構造や，熱測定で求められる増殖速度定数と最小増殖阻止濃度(MIC)などをもとに，酵母細胞に及ぼす一連のアルコールの影響を調べた．一連のアルコールについて，酵母に対するMICとオタマジャクシに対する麻酔作用のED50の間には良い直線関係がみられた．
74.	Yoshihisa Suzuki, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Tsutomu Sawada and Hiroshi Komatsu : Effects of pressure on growth kinetics of tetragonal lysozyme crystals, Journal of Crystal Growth, Vol.208, No.1-4, 638-644, 2000. (Summary) 高圧力下における正方晶系リゾチーム結晶の成長kineticsをその場測定により研究した．{110}面と{101}面の成長速度を0.1, 50, 100 MPaで測定した．両方の面とも，同じ過飽和度で比較した時，加圧により成長速度が減少した．二次元核成長のbirth and spread modelでfittingしたところ，平均ステップレッジ表面自由エネルギーが加圧とともに増加することが，成長速度の減少につながるということが明らかになった． (Link to Publication Site) ● Publication site (DOI): 10.1016/S0022-0248(99)00537-0 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0033890644 (DOI: 10.1016/S0022-0248(99)00537-0, Elsevier: Scopus)
75.	Gen Sazaki, Yukiko Nagatoshi, Yoshihisa Suzuki, Stephen Duen Durbin, Satoru Miyashita, Toshitaka Nakada and Hiroshi Komatsu : Solubility of tetragonal and orthorhombic lysozyme crystals under high pressure, Journal of Crystal Growth, Vol.196, 204-209, 1999. (Summary) マイケルソン干渉計を用いた溶解度の迅速測定法を高圧力下のリゾチームの溶解度測定に適用した．正方晶系リゾチームに関しては0.1, 50, 100 MPa，斜方晶系リゾチームに関しては，0.1, 100 MPaで温度の関数として溶解度を測定した．その結果，加圧に伴い，正方晶系の方は溶解度が増加し，それに対して斜方晶系では減少した．双方とも溶解エンタルピーは加圧に伴い減少した．
76.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita and Hiroshi Komatsu : In situ measurements of the solubility of crystals under high pressure by an interferometric method, The Review of Scientific Instruments, Vol.69, No.7, 2720-2724, 1998. (Summary) マッハツェンダー干渉計と光学窓付きの圧力容器を用いて，高圧力下での結晶の溶解度をその場測定した．この方法を用いれば，少量の材料で平衡化過程をその場観察できる．この手法をNH4Clの水に対する溶解度の圧力依存性の測定に適用し，他の手法による過去のデータと比較考察した．その結果，この手法は従来法に比べ，非常に少量のサンプルで測定でき，さらに精度の高い測定法であることが明らかになった．このことから，この手法はタンパク質のような，希少で高価な物質の溶解度を測定するのに適していると考えられる．
77.	Kazuo Kurihara, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Yoshihisa Suzuki and Hiroshi Komatsu : Interferometric study on the crystal growth of tetragonal lysozyme crystal, Journal of Crystal Growth, Vol.166, 904-908, 1996. (Summary) マイケルソン干渉計を用いて成長しているリゾチーム結晶の周りの濃度勾配を測定した．表面過飽和度(σsurf)を用いて結晶の成長モードを解析した結果，6<σsurf<20の間で二次元核成長モードであることが明らかになった．また，この広い過飽和度範囲において，リゾチームの拡散係数はほぼ一定で，4.9±0.7X10^(-11) m^2/sであることが分かった．
78.	Satoru Miyashita, Hiroshi Komatsu, Yoshihisa Suzuki and Toshitaka Nakada : Observation of the concentration distribution around a growing lysozyme crystal, Journal of Crystal Growth, Vol.141, 419-424, 1994. (Summary) マイケルソン干渉計を用いて，成長しているリゾチーム結晶の周りの濃度分布をその場観察した．結晶の成長速度と濃度分布からリゾチームの拡散係数は1.59X10^(-10) m^2/sと見積もることができた．また粘性率を測定して，結晶に取り込まれる溶質のストークス半径を求めた結果，結晶に取り込まれる溶質の大きさはリゾチーム単分子と等しく，リゾチームの単分子が結晶の成長単位であることがわかった．

Academic Paper (Unrefereed Paper):

1.	Yoshihisa Suzuki, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Taro Shimaoka, Masae Nagai, Koji Inaka, Shin-ichiro Yanagiya, Takehiko Sone, Shingo Wachi, Yasutomo Arai and Katsuo Tsukamoto : Highly Purified Glucose Isomerase Crystals Under Microgravity Conditions Grow as Fast as Those on the Ground Do, ChemRxiv, 2020. (Summary) Suppression of convection flows (solute transportation) and that of impurity incorporation into crystals seem to be the main reasons why the quality of protein crystals becomes better under microgravity conditions, whereas each precise mechanism has not been completely clarified yet. We tried to clarify the former reason by the in-situ observation of spiral growth hillocks on the {110} faces of highly purified glucose isomerase (GI) crystals under microgravity conditions and on the ground. Lateral growth rates Vlateral of a spiral hillock on the {110} face of a glucose isomerase crystal in situ under microgravity conditions and step velocities Vstep of the same configuration on the ground took similar values as far as the maximum values are compared each other. This similarity indicates there are less influences of the convection flows on the growth rates of protein crystals contrary to conventional expectations. (Keyword) crystal growth / nucleation / glucose isomerase / microgravity (Link to Publication Site) ● Publication site (DOI): 10.26434/chemrxiv.11808504.v1 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.26434/chemrxiv.11808504.v1 (DOI: 10.26434/chemrxiv.11808504.v1)

Academic Letter:

1.	Yoshihisa Suzuki, Hideaki Tsuge, Hironori Hondoh, Yusuke Kato, Yuta Uehara, Nobuo Maita, Kohei Hosokawa and Shoko Ueta : Precipitant-free lysozyme crystals grown by centrifugal concentration reveal structural changes, Crystal Growth & Design, Vol.18, No.8, 4226-4229, 2018. (Summary) The three-dimensional (3D) structure of a protein molecule in its crystal need not correspond to that found in vivo in many cases, since we usually crystallize protein molecules using precipitants (salts, organic solvents, polymeric electrolytes, etc.), and the precipitants are often incorporated into crystals along with the protein molecules. Although precipitant-free crystallization methods would solve these problems, such methods had not yet been established. We have achieved a novel precipitant-free crystallization method by liquid-liquid phase separation during the centrifugal concentration of lysozyme in ultra-pure water. In the 3D structure of the precipitant-free crystal, lysozyme loses a sodium cation and changes the position of Ser 72. Deionization of the solution also appears to induce a change in the position of Asp 101 and an increase in the activity of lysozyme. (Keyword) precipitant-free / lysozyme / centrifugal concentration / synchrotron x-ray crystallography (Tokushima University Institutional Repository) ● Metadata: 112469 (Link to Publication Site) ● Publication site (DOI): 10.1021/acs.cgd.8b00326 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85050030157 (Tokushima University Institutional Repository: 112469, DOI: 10.1021/acs.cgd.8b00326, Elsevier: Scopus)
2.	Atsushi Mori, Yoshihisa Suzuki and Masahide Sato : Gravitational Tempering in Colloidal Epitaxy To Reduce Defects Further, Crystal Growth & Design, Vol.14, No.5, 2083-2086, 2014. (Summary) Less-defective colloidal crystals can be used as photonic crystals. To this end, colloidal epitaxy was proposed in 1997 as a method to reduce the stacking defects in colloidal crystals. In this method, face-centered cubic (fcc) (001) stacking is forced by a template. In fcc (001) stacking, in contrast to fcc {111} stacking, the stacking sequence is unique, and thus the stacking fault can be avoided. Additionally, in 1997, an effect of gravity that reduces the stacking disorder in hard-sphere (HS) colloidal crystals was found. Recently, we have proposed a gravitational tempering method based on a result of Monte Carlo (MC) simulations using the HS model; after a colloidal crystal is grown in a relatively strong gravitational field, the defects can be reduced by decreasing the gravity strength and maintaining it for a period of time. Here, we demonstrate this method using MC simulations with a programmed gravitation. The dramatic disappearance of defect structures is observed. Gravitational tempering can complement gravitational annealing; some defect structures that accidentally remain after gravitational annealing (keeping the colloidal crystal under gravity of a considerable constant strength) can be erased. (Link to Publication Site) ● Publication site (DOI): 10.1021/cg401884k (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/cg401884k (DOI: 10.1021/cg401884k)
3.	Kaori Hashimoto, Atsushi Mori, Katsuhiro Tamura and Yoshihisa Suzuki : Enlargement of Grains of Silica Colloidal Crystals by Centrifugation in an Inverted-Triangle Internal-Shaped Container, Japanese Journal of Applied Physics, Vol.52, No.3, 030201-1-030201-3, 2013. (Summary) We successfully fabricated large grains of silica colloidal crystals in an inverted-triangle internal-shaped container (inverted-triangle container) by centrifugation. The largest grain in the container was much larger than that in a container which has a flat bottom and constant width (flat-bottomed container). The edged bottom of the inverted-triangle container eliminated the number of the grains, and then the broadened shape of the container effectively widened the grains. (Link to Publication Site) ● Publication site (DOI): 10.7567/JJAP.52.030201 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.7567/JJAP.52.030201 (DOI: 10.7567/JJAP.52.030201)
4.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Possibility of Gravitational Tempering in Colloidal Epitaxy to Obtain a Perfect Crystal, Chemistry Letters, Vol.41, No.10, 1069-1071, 2012. (Summary) We have performed Monte Carlo simulations of hard spheres on a pattern under gravity. We have found that a crystal formed at a moderate gravity strength contains essentially no defects while one formed at a higher strength gravity contains a significant amount of defects. This result suggests the possibility of using gravitational-tempering in a colloidal epitaxy to reduce the number of defects in colloidal crystals. Moreover, we wish to emphasize the possibility to obtain a perfect crystal. (Link to Publication Site) ● Publication site (DOI): 10.1246/cl.2012.1069 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2012.1069 (DOI: 10.1246/cl.2012.1069)
5.	Yoshihisa Suzuki, Gen Sazaki, Kalevi Visuri, Katsuhiro Tamura, Kazuo Nakajima and Shin-ichiro Yanagiya : Significant Decrease in the Solubility of Glucose Isomerase Crystals under High Pressure, Crystal Growth & Design, Vol.2, No.5, 321-324, 2002. (Summary) グルコースイソメラーゼ結晶の溶解度を0.1, 100 MPaでその場観察により測定した．具体的にはマイケルソン干渉計を用いてある濃度の溶液が結晶と平衡状態になるための平衡温度を，溶液濃度と圧力を変えて測定した．その結果，加圧に伴い溶解度が減少した．30℃で0.1から100 MPaまで加圧すると溶解度は1/9になった．このことはVisuriらによって報告された加圧による結晶化の促進を支持する． (Keyword) HIGH HYDROSTATIC-PRESSURE / D-XYLOSE ISOMERASE / LYSOZYME CRYSTALS / PROTEIN CRYSTALS / SUBTILISIN CRYSTALLIZATION / NUCLEATION KINETICS / GLOBULAR-PROTEINS / GROWTH / DEPENDENCE (Link to Publication Site) ● Publication site (DOI): 10.1021/cg025509f (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1571135651724474368 ● Search Scopus @ Elsevier (DOI): 10.1021/cg025509f (DOI: 10.1021/cg025509f, CiNii: 1571135651724474368)
6.	Tsutomu Sawada, Yoshihisa Suzuki, Akiko Toyotama and Nobuo Iyi : Quick Fabrication of Gigantic Single-Crystalline Colloidal Crystals for Photonic Crystal Applications, Japanese Journal of Applied Physics, Part 2 (Letters), Vol.40, No.11B, 1226-1228, 2001. (Summary) センチメーターサイズのコロイド単結晶ドメインを，瞬間的なシアーフローを用いた濃厚懸濁液の非平衡配列のクエンチによって作製した．得られたコロイド結晶は，決まった結晶学的方位をもち，外界からの擾乱に対して十分に安定であった．この方法は，3次元フォトニック結晶の作製，応用に役立つであろう．
7.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita, Hiroshi Komatsu, Gen Sazaki and Toshitaka Nakada : An interferometric study of the solubility of lysozyme crystals under high pressure, Journal of Crystal Growth, Vol.209, No.4, 1018-1022, 2000. (Summary) ニワトリ卵白リゾチーム正方晶系結晶に及ぼす圧力効果をマッハツェンダー干渉計と，我々のグループで新たにデザインした高圧光学セルを用いてその場観察により測定した．結晶の平衡化過程による濃度変化過程を，過飽和状態·未飽和状態の両方から正確にかつ連続的に測定した．溶解度の変化率はこれまでに測定されてきた値よりもずっと小さく，100 MPaで0.1 MPaの値の28%大きくなった． (Link to Publication Site) ● Publication site (DOI): 10.1016/S0022-0248(99)00746-0 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-0033906406 (DOI: 10.1016/S0022-0248(99)00746-0, Elsevier: Scopus)
8.	Yoshihisa Suzuki, Satoru Miyashita, Hiroshi Komatsu, Kiyotaka Sato and Takehiko Yagi : Crystal Growth of Hen Egg White Lysozyme under High Pressure, Japanese Journal of Applied Physics, Part 2 (Letters), Vol.33, 1568-1570, 1994. (Summary) ダイヤモンドアンビルセルを用いてニワトリ卵白リゾチームの結晶化に及ぼす静水圧の効果を研究した．結晶の核生成頻度と{110}, {101}面の成長速度を高圧力下でその場観察した．その結果，両方とも加圧に伴い減少した．観察された圧力効果はルシャトリエの原理で説明できる．
9.	Hiroshi Komatsu, Satoru Miyashita and Yoshihisa Suzuki : Interferometric Observation of the Interfacial Concentration Gradient Layers Around a Lysozyme Crystal, Japanese Journal of Applied Physics, Part 2 (Letters), Vol.32, 1855-1857, 1993. (Summary) 反射型マイケルソン干渉計を用いて，初めて，成長，もしくは溶解しているタンパク質結晶の表面モルフォロジー及び界面の濃度勾配層の観察に成功した．この方法を用いれば，溶液からの結晶の成長kineticsをうまく解析できるようになるであろう．

Review, Commentary:

1.	Yoshihisa Suzuki, Hideaki Tsuge, Shiori Fujiwara, Naoto Ikemitsu, Shoko Ueta and Takashi Sakai : Protein Crystal Growth Simply by Concentration, Journal of the Japanese Association for Crystal Growth, Vol.49, No.3, 05-1-05-9, Oct. 2022. (Summary) The three-dimensional (3D) structure of a protein molecule is usually solved by using crystals grown with copious amounts of precipitants. The obtained structure could be significantly different from those in vivo because the precipitant concentration in vivo is much less than those under normal crystallization conditions. For the first time, we have developed novel precipitant-free protein crystallization methods by centrifugal concentration and drying. For both methods, atomic-level 3D structures were successfully obtained. 3D molecular structure of hen egg-white lysozyme (HEWL) obtained without precipitants was significantly different from that obtained with precipitants, whereas the precipitant-free molecular structure of glucose isomerase (GI) was similar to those obtained with precipitants. (Keyword) Concentration / protein / crystal growth (Link to Publication Site) ● Publication site (DOI): 10.19009/jjacg.49-3-05 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.19009/jjacg.49-3-05 (DOI: 10.19009/jjacg.49-3-05)
2.	Yoshihisa Suzuki : 圧力によって深まる溶解現象の本質の理解, Chemistry and Education, Vol.69, No.3, 118-121, Mar. 2021. (Summary) 溶解現象は，様々な場面で重要な意味を持つことが多いが，本質的なところはまだ未解明な部分が多い．ここでは特に溶解に伴う体積変化と，そのメカニズムを解明するために有用な圧力の活用について，基本的なところから，創薬の分野等で必要となる高品質タンパク質結晶化に渡る部分まで紹介する． (Keyword) solubility / 部分モル体積 / 圧力依存性 / 活性化体積 (Link to Publication Site) ● Publication site (DOI): 10.20665/kakyoshi.69.3_118 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390010292494518656 ● Search Scopus @ Elsevier (DOI): 10.20665/kakyoshi.69.3_118 (DOI: 10.20665/kakyoshi.69.3_118, CiNii: 1390010292494518656)
3.	Yoshihisa Suzuki, Hitomi Minamizono, Kazuma Tsuchiya, Ai Ninomiya, Katsuo Tsukamoto and Yuko Inatomi : Effects of Gravity on the Growth Process of Colloidal Crystals with Depletion Force Mediated Attractive Interaction, Space Utilization Research, Vol.33, SA6000132021-1-SA6000132021-4, Jan. 2019. (Summary) Although depletion-induced attractive colloidal crystallization processes are recently studied as a model system of crystal growth because of the size of crystal unit (more than several hundred micro meters), effects of gravity would be significant, since the size and density of particles are large, and their crystallization usually starts at the bottom of growth containers. In this study we successfully observed in situ adsorption, desorption, surface diffusion, and kink incorporation of particles on growth interfaces of the depletion-induced attractive colloidal crystals of polystyrene particles in aqueous sodium polyacrylate solutions under several gravity conditions during parabolic flights. Precise surface transportation and kink incorporation processes of the particles into the colloidal crystals with attractive interactions were observed in situ at the particle level. Surface diffusion coefficients of particles " were roughly estimated. " at 0 G became larger than those at 1 G. " at 0 G also showed scattered data compared to those at 1 G, since particles which moved largely had a propensity to increase under microgravity conditions. Obtained " values were 1 ~ 2 orders of magnitude smaller than those of bulk diffusion coefficients. (Keyword) colloidal crystal / Depletion-induced attraction
4.	Yoshihisa Suzuki, Kazuma Tsuchiya, Takahisa Fujiwara, Katsuo Tsukamoto and Yuko Inatomi : Impurity Effects on the Step Velocity of a Spiral Hillock on a Crystal under Perfect Stagnant Conditions, Space Utilization Research, Vol.31, SA6000061011-1-SA6000061011-3, Jan. 2017. (Summary) Effects of gravity on step velocities step of hen egg-white lysozyme (HEWL) tetragonal crystals are studied. Parabolic flight experiments revealed that step in <110> directions of the crystals have a tendency to increase with a decrease in gravity. This increase is mainly due to the decrease in convection flow rates, since the decrease would result in the decrease in the transportation rates of covalently bound dimer of HEWL molecules (major impurity of this study) onto the growth interface of the crystals. (Keyword) Step velocity / In situ observation
5.	Junpei Yamanaka, Yoshihisa Suzuki, Jun Nozawa and Tsutomu Sawada : In-situ observation of colloidal crystallization, Progress in Crystal Growth and Characterization of Materials, Vol.62, No.2, 413-416, Apr. 2016. (Summary) We report contents of colloidal crystallization experiments in ISSCG-16. The participants produce two kinds of colloidal crystals, i.e., close-packed opal type crystals, and non-close-packed charged colloidal crystals. They can observe the crystallization processes by using optical microscopy and reflection spectroscopy. (Keyword) Kinetics (Link to Publication Site) ● Publication site (DOI): 10.1016/j.pcrysgrow.2016.04.025 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84976506697 (DOI: 10.1016/j.pcrysgrow.2016.04.025, Elsevier: Scopus)
6.	Yoshihisa Suzuki and Takahisa Fujiwara : Hydration / Dehydration Processes from the Viewpoints of Effects of Pressure on Solubilities and Growth Rates of Crystals, Journal of the Japanese Association for Crystal Growth, Vol.42, No.4, 274-282, Jan. 2016. (Summary) Precise understandings of hydration and dehydration processes in crystallization processes are prerequisites for deeper insights into fundamentals of crystal growth in aqueous solutions. In this review, we present the great potentialities of thermodynamic and kinetic analyses of the dependence of solubilities and crystal-growth-rates on pressure on experimentally accessible information of hydration and hydration processes in crystallization processes. The dependence of solubility of crystals on pressure provides molar volume change of dissolution. Positive and negative slopes of solubility curves with pressure result in the decrease and increase in the molar volume of solutes during dissolution processes, respectively. Activation volumes during solute incorporation processes at kink sites are calculated using the dependence of step kinetic constant on pressure. In practice, solubilities of alkali halide, amino acid, and protein crystals, and step velocity of glucose isomerase crystals are discussed. (Link to Publication Site) ● Publication site (DOI): 10.19009/jjacg.42.4_274 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205897221760 ● Search Scopus @ Elsevier (DOI): 10.19009/jjacg.42.4_274 (DOI: 10.19009/jjacg.42.4_274, CiNii: 1390001205897221760)
7.	Yoshihisa Suzuki, Katsuo Tsukamoto, Takahisa Fujiwara, Katsuo Tsukamoto and Shin-ichiro Yanagiya : Step Velocities of Protein Crystals in Ground-based Convection Free Experiments, Space Utilization Research, Vol.30, SA6000048034-1-SA6000048034-4, Jan. 2016. (Summary) Convection flows induced by formation of solute depletion zone around growing protein crystals are visualized to clarify the reason why step velocities of a hen egg-white lysozyme (HEWL) tetragonal crystal grown at a ceiling position (top wall of a growth container) are not different from those at a sitting position (bottom wall of the container). Parabolic flight experiments revealed that flow rates of polystyrene particles (as marker particles) around a ceiling crystal do not indicate zero at hyper gravity conditions, and the particles completely stop at zero gravity, although the flow rates show slightly less values than those around a sitting crystal. Ceiling experiments cannot reproduce the effective suppression of convection flows around growing protein crystals in microgravity at this stage. Thus microgravity experiments are still indispensable to attain complete convection free conditions. (Keyword) Step velocity / In situ observation
8.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama, Takahisa Fujiwara, Shin-ichiro Yanagiya, Masaru Tachibana and Haruhiko Koizumi : Change in interface growth kinetics and interface morphology of a protein crystal, Space Utilization Research, Vol.29, No.3, 53-56, Jan. 2015. (Summary) We focus on the growth mechanism of protein crystals and their perfection. The growth mechanism will be studied by surface observation and by measuring growth rates. The crystal defect will be studied by X-ray topography. We successfully carried out Nanostep project as an international space station (ISS) experiment in 2012 using lysozyme crystals as model protein crystals. From the results of the Nanostep project, we concluded that larger-size impurities are effectively filtered during crystallization owing to smaller diffusion constants of the larger impurities, and this filtration mechanism results in the increase in growth rate of lysozyme crystals in microgravity. However, from this assumption, we can easily anticipate that same-size impurities will not lead the faster growth rates, and smaller impurities will lead more suppression of growth rates in microgravity. To confirm these anticipations, a new ISS experiment is planned in near future using glucose isomerase (GI) crystals. We need to obtain ground based data for the comparison with the Nanostep data. Until now, we successfully confirm regrowth of GI crystals on chemically fixed seed crystals, conduct X-ray topography of regrown crystals on the seed crystals, and observe elementary steps on ceiling lysozyme crystals.
9.	Katsuo Tsukamoto, Hitoshi Miura, Yoshihisa Suzuki, Kenta Murayama, Takahisa Fujiwara and Izumi Yoshizaki : Exact Measument of Crystal Growth Rate of Lysozyme on The Ground and in ``Kobo'', Space Utilization Research, Vol.29, No.3, 35-37, Jan. 2015. (Summary) Growth rate of lysozyme crystals vs supersaturation has exactly been measured for the first time by means of laser interferometry at Kibo in odder to analyze the optimum condition and/or the growth mechanism for good quality protein crystals. The growth rate and shape of spiral growth hillocks were compared as obtained in gravity using the same growth cells. We here found the larger growth rate under microgravity than the rate in gravity in the wide range of supersaturation, which has commonly been used for growing crystals in gravity. The higher growth rate in space is attributed to the suppression of the adsorption of dimer-molecules that are present as impurity molecules, which block step movement along specific crystallographic directions depending on supersaturation. The lack of 2D heterogeneous nucleation growth regime in space is also due to the reduction of this impurity concentration at the growth surface. These effects would eventually widen the growth regime that is suitable for getting high quality protein crystals.
10.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, Seijiro Fukuyama, Masaru Tachibana, Haruhiko Koizumi, Takahisa Fujiwara, Hiroaki Shiozawa, Yuta Uehara, Tomohiro Shiomoto and Daido Nakahashi : Effects of Microgravity on Crystallizaion of Glucose Isomerase, --- Collaborative Studies toward the Proposal for Nanostep 2 Project ---, International Journal of Microgravity Science and Application, Vol.31, No.3, 124-129, Jun. 2014. (Summary) Pressure is an attractive thermodynamic parameter. In this review, I present the great potentialities of high pressure for the promotion of studies on the enhancement of protein crystallization processes and correlation between functions and 3D structures of protein molecules. As a tool for enhancing the crystal growth, three of eight proteins show the decrease in its solubility under high pressure. Acceleration of growth and nucleation kinetics of glucose isomerase crystals occurred under high pressure. Step velocities under high pressure provided us direct information on activation volumes during incorporation processes of protein molecules at a kink site of crystals. Such activation volumes were negative in the case of glucose isomerase crystals. Precise discussion on the activation volume will be useful for understanding dehydration mechanisms during the incorporation processes. Usefulness of our standalone-type Be vessel for high-pressure protein crystallography was confirmed. With the vessel, precise high-pressure 3D structure analysis of protein crystals which are also grown under high pressure will be achieved. (Keyword) crystal growth / surface morphology / growth rates / in situ observation (Link to Publication Site) ● Publication site (DOI): 10.15011/jasma.31.3.124 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390003825180821120 ● Search Scopus @ Elsevier (DOI): 10.15011/jasma.31.3.124 (DOI: 10.15011/jasma.31.3.124, CiNii: 1390003825180821120)
11.	Yoshihisa Suzuki, Gen Sazaki, Takahisa Fujiwara and Masayuki Tsukamoto : Pressure effects on crystal growth of proteins, Journal of the Japanese Association for Crystal Growth, Vol.40, No.2, 107-114, Jul. 2013. (Summary) Pressure is an attractive thermodynamic parameter. In this review, I present the great potentialities of high pressure for the promotion of studies on the enhancement of protein crystallization processes and correlation between functions and 3D structures of protein molecules. As a tool for enhancing the crystal growth, three of eight proteins show the decrease in its solubility under high pressure. Acceleration of growth and nucleation kinetics of glucose isomerase crystals occurred under high pressure. Step velocities under high pressure provided us direct information on activation volumes during incorporation processes of protein molecules at a kink site of crystals. Such activation volumes were negative in the case of glucose isomerase crystals. Precise discussion on the activation volume will be useful for understanding dehydration mechanisms during the incorporation processes. Usefulness of our standalone-type Be vessel for high-pressure protein crystallography was confirmed. With the vessel, precise high-pressure 3D structure analysis of protein crystals which are also grown under high pressure will be achieved. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001205896471040 (CiNii: 1390001205896471040)
12.	Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba and Katsuhiro Tamura : X-ray structure analysis of glucose isomerase molecules in crystals prepared under high pressure, 高圧バイオサイエンスとバイオテクノロジー第15回生物関連高圧研究会20周年記念シンポジウム抄録集, Vol.2, 29-37, Sep. 2008.
13.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : In Situ Observation of Molecular Steps on the Surface of Protein Crystal under High Pressure, The Review of High Pressure Science and Technology, Vol.17, No.1, 50-56, Feb. 2007. (Summary) Velocities of elementary steps and two-dimensional nucleation rates on the {101} face of glucose isomerase (GI) crystals were measured at pressures of 0.1, 25 and 50 MPa. We conducted in situ observation of the steps by using a laser confocal microscope combined with differential interference contrast microscope (LCM-DIM) and high-pressure vessel with a sapphire window (t = 1 mm). Both step velocities and 2D nucleation rates increased with increasing pressure at T = 26.4°C and C = 5.6 mg ml-1. Solubilities Ce at high pressures were also measured by in situ observation of the moving steps around equilibrium temperatures. The solubility decreased with increasing pressure. (Keyword) glucose isomerase / solubility / step velocity / laser confocal microscope / differencial interference contrast microscope / in situ observation / two-dimensional nucleation (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.17.50 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001204382136448 ● Summary page in Scopus @ Elsevier: 2-s2.0-34247222623 (DOI: 10.4131/jshpreview.17.50, CiNii: 1390001204382136448, Elsevier: Scopus)
14.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : 圧力印加による酵素の結晶成長の促進, 食品と容器, Vol.47, No.6, 320-327, Jun. 2006.
15.	Gen Sazaki, Yoshihisa Suzuki, Satoru Miyashita, Tsutomu Sawada, Hiroshi Komatsu, Katsuhiro Tamura and Kazuo Nakajima : タンパク質の結晶化に及ぼす圧力効果, 日本結晶学会誌, Vol.46, No.2, 152-158, Jan. 2004. (Summary) 静水圧はタンパク質の結晶化を制御するのに有用なパラメタである．タンパク質結晶の溶解度は，圧力を変化させることで急速に変化させることが可能である．タンパク質分子の特異的な表面に対する水和に関する情報は，溶解度の圧力依存性から得られる．タンパク質分子が疎水的であるか，その溶液中の分子体積が結晶中よりも十分大きい時，圧力印加は，結晶化を促進する効果的な手法となる．圧力はまた，タンパク質結晶の成長kineticsにも影響を及ぼす．典型的なタンパク質について，成長速度の結晶化駆動力に対する依存性を測定し，二次元核成長の多核成長様式を用いて解析した．成長kineticsに及ぼす高圧力効果は，結晶の表面エネルギー，分子が結晶に取り込まれる過程における活性化エネルギー，そして結晶表面のステップ上の平均キンク間距離の変化で説明出来る．しかし，成長kineticsに及ぼす圧力効果に対して，一般的·普遍的な解釈はまだ得られてはいない．
16.	Gen Sazaki, Shin-ichiro Yanagiya, Yoshihisa Suzuki, Satoru Miyashita, Takao Sato, Yoshiki Matsuura, Takuro Matsui and Kazuo Nakajima : A Novel Approach to Protein Crystallization : Use of a Magnetic Field and High-Pressure, Seibutsu Butsuri, Vol.44, No.2, 58-63, Jan. 2004. (Summary) A magnetic field and high-pressure are effective parameters to control protein crystallization. A magnetic field significantly affects the number, orientation and growth rate (morphology) of protein crystals and damps the buoyancy convection in protein solutions. Quality of protein crystals can be also improved by a magnetic field. The improvement in the crystal quality is due to a magnetic orientation effect. High-pressure can also give large effects on the solubility and growth kinetics of protein crystals. Changes in the solubility with pressure results from a difference in the volume between bulk water and the water hydrating the contact regions of protein molecules inside the crystal. (Keyword) magnetic field / high-pressure / protein / crystallization (Link to Publication Site) ● Publication site (DOI): 10.2142/biophys.44.58 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390001206533751168 ● Search Scopus @ Elsevier (DOI): 10.2142/biophys.44.58 (DOI: 10.2142/biophys.44.58, CiNii: 1390001206533751168)
17.	Yoshihisa Suzuki, Gen Sazaki, Tsutomu Sawada, Satoru Miyashita, Hiroshi Komatsu and Katsuhiro Tamura : Protein Crystallization under High Hydrostatic Pressure, The Review of High Pressure Science and Technology, Vol.13, No.2, 149-156, May 2003. (Summary) In this article, recent advances in the fundamental research in the protein crystallization under high pressure were reviewed. Pressure is expected to be an important parameter to control protein crystallization, since hydrostatic pressure affects the whole system uniformly and can be changed very rapidly. So far, a lot of studies of protein crystallization under high pressure have been done, some less systematic than others. We have particularly focused on fundamental aspects (the solubility and the crystal growth kinetics) of protein crystallization under high pressure. The studies reviewed contribute significantly to high-pressure crystallography and high-pressure protein science. (Keyword) protein crystallization / X-ray crystallography / in situ observation / solubility / crystal growth rate / growth kinetics / nucleation rate / supersaturation / optical microscope / interferometer (Link to Publication Site) ● Publication site (DOI): 10.4131/jshpreview.13.149 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679357126784 ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.13.149 (DOI: 10.4131/jshpreview.13.149, CiNii: 1390282679357126784)
18.	Yoshihisa Suzuki, Gen Sazaki, Satoru Miyashita, Tsutomu Sawada, Katsuhiro Tamura and Hiroshi Komatsu : Protein crystallization under high pressure, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol.1595, No.1, 345-356, Mar. 2002. (Summary) Pressure is expected to be an important parameter to control protein crystallization, since hydrostatic pressure affects the whole system uniformly and can be changed very rapidly. So far, a lot of studies on protein crystallization have been done. Solubility of protein depends on pressure. For instance, the solubility of tetragonal lysozyme crystal increased with increasing pressure, while that of orthorhombic crystal decreased. The solubility of subtilisin increased with increasing pressure. Crystal growth rates of protein also depend on pressure. The growth rate of glucose isomerase was significantly enhanced with increasing pressure. The growth rate of tetragonal lysozyme crystal and subtilisin decreased with increasing pressure. To study the effects of pressure on the crystallization more precisely and systematically, hen egg white lysozyme is the most suitable protein at this stage, since a lot of data can be used. We focused on growth kinetics under high pressure, since extensive studies on growth kinetics have already been done at atmospheric pressure, and almost all of them have explained the growth mechanisms well. The growth rates of tetragonal lysozyme decreased with pressure under the same supersaturation. This means that the surface growth kinetics significantly depends on pressure. By analyzing the dependence of supersaturation on growth rate, it was found that the increase in average ledge surface energy of the two-dimensional nuclei with pressure explained the decrease in growth rate. At this stage, it is not clear whether the increase in surface energy with increasing pressure is the main reason or not. Fundamental studies on protein crystallization under high pressure will be useful for high pressure crystallography and high pressure protein science. (Keyword) Animals / Crystallization / Hydrostatic Pressure / Kinetics / Muramidase / Solubility (Link to Publication Site) ● Publication site (DOI): 10.1016/S0167-4838(01)00355-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11983407 ● Search Scopus @ Elsevier (PMID): 11983407 ● Search Scopus @ Elsevier (DOI): 10.1016/S0167-4838(01)00355-7 (DOI: 10.1016/S0167-4838(01)00355-7, PubMed: 11983407)
19.	Satoru Miyashita, Gen Sazaki, Yukiko Nagatoshi, Yoshihisa Suzuki, Tsutomu Sawada, Toshitaka Nakada, Hiroshi Komatsu and Kazuo Nakajima : Pressure Effects of Crystallization of Hen Egg White Lysozyme, Journal of the Japanese Association for Crystal Growth, Vol.26, No.4, 192-202, Jul. 1999. (Summary) タンパク質の結晶化に及ぼす圧力効果についてニワトリ卵白リゾチームをモデルタンパクとして研究した．ダイヤモンドアンビルセルを用いた直接観察により，加圧に伴う正方晶系結晶の溶解度の増加と，核生成頻度·結晶成長速度の減少が明らかになった．定量的な研究を行うために，高圧力下で二光束干渉計を用いた新規な溶解度測定法を開発した．その結果，正方晶系結晶では加圧に伴い溶解度が増加したのに対し，斜方晶系結晶では減少した．この傾向については，結晶中の分子結合サイトに対する水和を考えることで議論した．結晶の成長速度を同じ結晶化駆動力で比較しても高圧力下の方が結晶成長速度が遅くなり，圧力は速度論的にも結晶化に影響を及ぼすことが明らかになった．これは正方晶系，斜方晶系とも同様であった．二次元核成長モデルで成長速度の解析を行った結果，正方晶系では，加圧に伴う結晶の平均ステップレッジ表面自由エネルギーの増加が成長速度の減少の原因であることが分かった．

Proceeding of International Conference:

1.	Ryusei Fukunaga, Masahide Sato and Yoshihisa Suzuki : In-situ observation of homogeneous nucleation of colloidal crystals formed with attractive interactions under density-matching conditions, 4th International Conference on Nanomaterials and Advanced Composites (NAC), Nov. 2023. (Keyword) colloidal crystal / nucleation / depletion aggregation / heavy water
2.	Yoshihisa Suzuki, Akiho Ikeuchi and Koichiro Taoka : Solution-mediated phase transition of protein crystals, 4th International Conference on Nanomaterials and Advanced Composites (NAC), Nov. 2023. (Keyword) in situ observation / Solution-mediated phase transition / glucose isomerase / evapolation
3.	Masahiro Kanno, Yutaka Tsuboi, Shigeki Matsuo and Yoshihisa Suzuki : Estimation of the activation energy of kink incorporation of particles into colloidal crystals whose particle interaction is attractive, 3rd International Conference on Nanomaterials and Advanced Composites, Tokushima, Jul. 2022.
4.	Yoshihisa Suzuki, Ai Ninomiya, Yutaka Tsuboi, Masahiro Kanno, Shin-ichiro Yanagiya and Shigeki Matsuo : In situ observation of crystal growth processes, 3rd International Conference on Nanomaterials and Advanced Composites, Tokushima, Jul. 2022. (Link to Publication Site) ● Publication site (DOI): 10.1007/978-981-99-7153-4_8 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85178507628 (DOI: 10.1007/978-981-99-7153-4_8, Elsevier: Scopus)
5.	Yoshihisa Suzuki and Nobuo Maita : Precipitant-Free Crystallization of Glucose Isomerase Simply by Concentration in a Cryoprotectant Solution, 19th International Conference on Crystal Growth and Epitaxy, Keystone, Aug. 2019.
6.	Hitomi Minamizono and Yoshihisa Suzuki : In situ observation of growth process of polystyrene colloidal crystal with depletion force, International Symposium & School on Crystal Growth Fundamentals, Sendai, Nov. 2018.
7.	Yoshihisa Suzuki, Takahisa Fujiwara, Hideaki Tsuge, Hironori Hondoh, Yusuke Kato, Yuta Uehara, Nobuo Maita, Kohei Hosokawa and Shoko Ueta : Precipitant-free crystallization of proteins, International Symposium & School on Crystal Growth Fundamentals, Sendai, Nov. 2018.
8.	Katsuo Tsukamoto, Hitoshi Miura, Yoshihisa Suzuki and Izumi Yoshizaki : In-Situ Measurement of Lysozyme Crystal Growth Rate vs Supersaturation with different impurity level in the International Space Station, 18th International Conference on Crystal Growth and Epitaxy, Nagoya, Aug. 2016.
9.	Yoshihisa Suzuki, Takahisa Fujiwara and Shoko Ueta : Protein Crystallization by Slow Evaporation of Precipitant-free Solution in the Presence of Hydrochloric Acid, 18th International Conference on Crystal Growth and Epitaxy, Nagoya, Aug. 2016.
10.	Ryo Suzuki, Takeharu Kishi, Haruhiko Koizumi, Katsuo Tsukamoto, Yasutomo Arai, Seijiro Fukuyama, Yoshihisa Suzuki, Kenichi Kojima and Masaru Tachibana : Characterization of grown-in dislocation in high quality glucose isomerase crystals by synchrotron X-ray topography, 18th International Conference on Crystal Growth and Epitaxy, Vol.468, 299-304, Nagoya, Aug. 2016. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jcrysgro.2016.10.047 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85007110614 (DOI: 10.1016/j.jcrysgro.2016.10.047, Elsevier: Scopus)
11.	Takahisa Fujiwara, Daido Nakahashi, Shin-ichiro Yanagiya and Yoshihisa Suzuki : Step velocities of glucose isomerase crystals in the presence of hen egg-white lysozyme in solution, 18th International Conference on Crystal Growth and Epitaxy, Nagoya, Aug. 2016.
12.	Yoshihisa Suzuki, Katsuo Tsukamoto, Takahisa Fujiwara, Tomohiro Shiomoto, Daido Nakahashi, Izumi Yoshizaki, Seijiro Fukuyama, Masaru Tachibana, Haruhiko Koizumi, Shin-ichiro Yanagiya, Yasutomo Arai and Makoto Natuisaka : Recent Advances on the Ground-Based Experiments of Protein Crystallization after the NanoStep Project, The Joint Conference of 6th International Symposium on Phshycs in Space & 10th Inerntional Conference on Two-Phase System for Space and Ground Applications, Kyoto, Sep. 2015.
13.	Haruhiko Koizumi, Masaru Tachibana, Izumi Yoshizaki, Seijiro Fukuyama, Katsuo Tsukamoto, Yoshihisa Suzuki, Satoshi Uda and Kenichi Kojima : Dislocations in High-quality Glucose Isomerase Crystals Grown from Seed Crystals, The Joint Conference of 6th International Symposium on Phshycs in Space & 10th Inerntional Conference on Two-Phase System for Space and Ground Applications, Kyoto, Sep. 2015. (Link to Publication Site) ● Publication site (DOI): 10.1021/cg500731v (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1021/cg500731v (DOI: 10.1021/cg500731v)
14.	Masahide Sato, Mamoru Fujine, Hiroyasu Katsuno and Yoshihisa Suzuki : Effect of Container Shape and Force Direction on Crystallization of Brownian Particles in Sedimentation, The Joint Conference of 6th International Symposium on Phshycs in Space & 10th Inerntional Conference on Two-Phase System for Space and Ground Applications, Kyoto, Sep. 2015.
15.	Atsushi Mori, Yoshihisa Suzuki and Masahide Sato : Some Details of Gravitational Tempering in Colloidal Epitaxy Using Hard-Sphre Model, The Joint Conference of 6th International Symposium on Physics in Space & 10th International Conference on Two-Phase System for Space and Ground Applications, 17, Kyoto, Sep. 2015.
16.	AlexanderE.S. VanDriessche, Mike Sleutel, James Lutsko, Dominique Maes, Katsuo Tsukamoto, Yoshihisa Suzuki and Izumi Yoshizaki : New Insights on Impurity Effects During Crystal Growth Under Various Gravity Conditions, The Joint Conference of 6th International Symposium on Phshycs in Space & 10th Inerntional Conference on Two-Phase System for Space and Ground Applications, Kyoto, Sep. 2015.
17.	Yoshihisa Suzuki, Masayuki Tsukamoto, Takahisa Fujiwara and Yuta Uehara : High pressure crystallization and crystallography of glucose isomerase, 23rd Congress and General Assembly of the International Union of Crystallography (IUCr2014), Montreal, Aug. 2014.
18.	Sho Kawakami, Atsushi Mori, Yoshihisa Suzuki, Shuichi Hashimoto, Masanobu Haraguchi and Tsutomu Sawada : Nanostructure for Localized Surface Plasmon Coupled with Photonic Band of Gel-Immobilized Colloidal Photonic Crystal, Proceedings of the JSME/ASME 2014 International Conferenec on Materials and Processing, ICPM2014, Detroit, Jun. 2014. (Summary) Gel-immobilized colloidal crystals were immersed into an aqueous dispersion of gold nanoparticles to fabricate nanostructures exhibiting an enhancement of optical electric field due to coupling of a localized surface plasmon and a photonic crystal with a tunable photonic band structure. We examined various immersion periods. Various reflection spectra depending on the immersion period were obtained. Keywords: Localized surface plasmon, Photonic crystal, Gelimmobilized colloidal crystal
19.	Sho Kawakami, Atsushi Mori and Yoshihisa Suzuki : Gravitational Tempering in Colloidal Epitaxy, Proceedings of the JSME/ASME 2014 International Conferenec on Materials and Processing, ICPM2014, Detroit, Jun. 2014. (Summary) Recently, we proposed gravitational tempering in colloidal epitaxy, in which the strength of gravity is once decreased and then kept for a certain period after the colloidal crystal was grown at gravity of a relatively high strength (gravitational annealing), to erase some defects remained in gravitational annealing. Results of Monte Carlo simulations, which demonstrate this method, will be shown.
20.	Yoshihisa Suzuki, Jin Endoh, Yohei Hamada, Atsushi Mori, Masahide Sato and Hiroyasu Katsuno : Crystallization and annealing of colloidal crystals under gravitational field, The 17th International Conference on Crystal Growth and Epitaxy, Warsaw, Aug. 2013.
21.	Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : Ordering of Brownian Particles from Walls Due to an External Force, The 17th International Conference on Crystal Growth and Epitaxy, Warsaw, Aug. 2013.
22.	Katsuo Tsukamoto, Izumi Yoshizaki, Yoshihisa Suzuki, Hitoshi Miura, Gen Sazaki, Masaru Tachibana, Kentaro Oshi and Manuel Juan Garcia-Ruiz : Growth Mechanism of Lysozyme Crystals in The International Space Station Based on The Analysis of In-Situ Interferometric Observation, The 17th International Conference on Crystal Growth and Epitaxy, Warsaw, Aug. 2013.
23.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Monte Carlo Simulation of Gravitational Temperig in Colloidal Epitaxy, International Topical Team on Crystal Growth, Colloidal Crystallization and Protein Crystallization, Sendai, Mar. 2013.
24.	Yuki Miyauchi, Satoshi Kawachi, Yoshihisa Suzuki, Yasuhiro Uosaki and Katsuhiro Tamura : Sterilization of satsuma mandarin juice by oxygen-nitrogen gas hybrid pressurization, The 7th International Conference on High Pressure Bioscience and Biotechnology, Otsu, Nov. 2012.
25.	Takahisa Fujiwara, Gen Sazaki, Shin-ichiro Yanagiya and Yoshihisa Suzuki : Activation volume of crystallization of tetragonal lysozyme crystals, The 7th International Conference on High Pressure Bioscience and Biotechnology, Otsu, Nov. 2012.
26.	Satoshi Kawachi, Yoshihisa Suzuki, Yasuhiro Uosaki and Katsuhiro Tamura : Effect of high-pressure gases on the growth of Escherichia coli determined by microcalorimetry, The 7th International Conference on High Pressure Bioscience and Biotechnology, Otsu, Oct. 2012.
27.	Yohei Yamada, Shota Toyama, Takahito Suzuta, James Metson, David Williams, Yoshihisa Suzuki, Tomoki Yabutani and Toshio Takayanagi : Characterization of penetration of metallic oxide precursor into ferritin crystal as a template for highly orderedmesoporous materials, JAIMA Discussion on Analytical Science and Technology 2012, The Royal Society of Chemistry Tokyo International Conference 2012, Chiba, Sep. 2012.
28.	Yoshihisa Suzuki : Enlargement and annealing of a single colloidal crystal grain, JANE-2012 Japan-Netherlands Symposium on Crystal Growth -Theory and in situ Measurements-, Sendai, Jul. 2012.
29.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Monte Carlo simulation of improvement of crystallinity in colloidal epitaxy on square pattern under gravitational field using hard-sphere model, 14th International Association of colloid and Interface Scientist, Conference, Sendai, May 2012.
30.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : Step Velocity On The {110} Faces Of Tetragonal Lysozyme Crystals Under High Pressure, Nineteenth Annual International Coference on Composite or Nano Engineering, Shanghai, Jul. 2011.
31.	Makiko Sakabe, Yoshihisa Suzuki, Takahisa Fujiwara, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Observation Of Mobile Lattice Defects In A Colloidal Crystal, Nineteenth Annual International Conference on Composite or Nano Engineering, Shanghai, Jul. 2011.
32.	Kaori Hashimoto, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Enlargement Of Grains Of Silica Colloidal Crystals By Centrifugation With An Inverted- Triangle Shaped Container, Nineteenth Annual International Conference on Composite or Nano Engineering, Shanghai, Jul. 2011.
33.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : Monte Carlo Simulation Of Defects In Hard-Sphere Crystal Grown On A Square Pattern, Nineteenth Annual International Conference on Composite or Nano Engineering, Shanghai, Jul. 2011.
34.	Jin Endoh, Yoshihisa Suzuki, Atsushi Mori, Tomoki Yabutani and Katsuhiro Tamura : Gravitational Annealing of Colloidal Crystals, 8th International Conference in Diffusion in Materials, Dijion, Jul. 2011. (Keyword) crystal growth
35.	Yoshihisa Suzuki, Masayuki Tsukamoto, Haruhiko Sakuraba, Masamitsu Matsumoto, Makoto Nagasawa and Katsuhiro Tamura : Design of a stand-alone type high-pressure vessel for protein crystallography, Program and abstract book 6th International Conference on High Pressure Bioscience and Biotechnology, 95, Freising, Aug. 2010.
36.	Satoshi Kawachi, Katsuhiro Tamura, Yoshihisa Muramoto, Hitoshi Iwahashi and Yoshihisa Suzuki : Development of food sterilization equipment by oxygen-nitrogen gas hyprid pressurization system, Program and abstract book 6th International Conference on High Pressure Bioscience and Biotechnology, 91, Freising, Aug. 2010.
37.	Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba and Katsuhiro Tamura : Compressed crystal structure of glucose isomerase determined by high-pressure crystallography, Program and abstract book 6th International Conference on High Pressure Bioscience and Biotechnology, 63, Freising, Aug. 2010.
38.	Yoshihisa Suzuki, Takeshi Maruoka and Katsuhiro Tamura : Activation volume of crystallization and effects of pressure on the three-dimensional nucleation rate of glucose isomerase crystals, Program and abstract book 6th International Conference on High Pressure Bioscience and Biotechnology, 62, Freising, Aug. 2010.
39.	Yoshihisa Suzuki, Atsushi Mori, Tsutomu Sawada and Katsuhiro Tamura : Fabrication of bulk close-packed colloidal crystals by centrifugation with the control of substrate angles, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
40.	Yoshihisa Suzuki, Tsutomu Sawada, Atsushi Mori, Harutoshi Asakawa and Katsuhiro Tamura : Grain-size control of a pilllar-like colloidal crystal by centrifugation, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
41.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : SEM observations of the fracture surface of a close-packed colloidal crystal, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
42.	Yoshihisa Suzuki, Tsutomu Sawada and Katsuhiro Tamura : Colloical crystallization by centrifugation method, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
43.	Makiko Sakabe, Yoshihisa Suzuki, Takahisa Fujiwara, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Relaxation of mobile lattice defects in a colloidal crystal, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
44.	Kaori Hashimoto, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Elimination and enlargement of grains of silica colloidal crystals by centrifugation with an inverted-triangle shaped container, Program book of the 16th International Conference on Crystal Growth, 112, Beijing, Aug. 2010.
45.	Yoshihisa Suzuki, Emi Konda, Hironori Hondoh and Katsuhiro Tamura : Effects of temperature, pressure, and pH on the solubility of triclinic lysozyme crystals, Program book of the 16th International Conference on Crystal Growth, 113, Beijing, Aug. 2010.
46.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : Effects of pressure on the solubility and thermodynamic parameters of tetragonal lysozyme crystals, Program and abstract book 6th International Conference on High Pressure Bioscience and Biotechnology, 16, Bristol, Aug. 2010.
47.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : Effects of pressure on the step velocity of tetragonal lysozyme crystals, Program book of the 16th International Conference on Crystal Growth, 112, Beijing, Aug. 2010.
48.	Satoshi Kawachi, Toshiaki Arao, Yoshio Hara, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of compression with ethane, ethylene and their fluorinated derivatives on yeast growth, Journal of Physics: Conference Series, Vol.215, No.1, 012168-1-012168-5, Apr. 2010. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012168 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012168 (DOI: 10.1088/1742-6596/215/1/012168)
49.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki and Katsuhiro Tamura : Solubility measurements of protein crystals under high pressure by in situ observation of steps on crystal surfaces, Journal of Physics: Conference Series, Vol.215, No.1, 012159-1-012159-5, Bristol, Apr. 2010. (Summary) We successfully measured equilibrium temperatures Te of tetragonal hen egg-white lysozyme crystals under high pressure by in situ observation of steps on the {110} faces of the crystals. The dependence of Te on the concentration of lysozyme corresponds to that of the solubility Ce on temperature. The precision of the solubility determined in this study is significantly higher than that in previous works. One Te could be measured during short time less than 70 minutes. This method for solubility measurements of protein crystals under high pressure is the fastest one at this stage. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012159 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012159 (DOI: 10.1088/1742-6596/215/1/012159)
50.	Takeshi Maruoka, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of High Pressure on the Three-Dimensional Nucleation Rates of Glucose Isomerase Crystals, Journal of Physics: Conference Series, Vol.215, No.1, 012158-1-012158-5, Bristol, Apr. 2010. (Summary) Three-dimensional nucleation rates J and induction times τ of glucose isomerase (GI) crystals were measured by counting the number of observable micro crystals with time in situ under high pressure. We also determined the solubility Ce under high pressure from the dependence of the growth and dissolution rates of the crystals on GI concentration C, and calculated supersaturation σ ( σ ≡ ln(C / Ce)) under high pressure. Both J and τ-1 increased with increasing pressure at the same σ. This indicates that the nucleation of the GI crystals was kinetically accelerated with pressure. (Link to Publication Site) ● Publication site (DOI): 10.1088/1742-6596/215/1/012158 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012158 (DOI: 10.1088/1742-6596/215/1/012158)
51.	Satoshi Kawachi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of some compressed unsaturated hydrocarbon gases on yeast growth, Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology, 87, San Diego, Sep. 2008.
52.	Katsuhiro Tamura, Yoshihisa Muramoto, Toshiaki Arao, Yoshihisa Suzuki and Hitoshi Iwahashi : Development of food sterilization equipment by oxygen-nitrogen gas hyprid pressurization system, Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology, 86, San Diego, Sep. 2008.
53.	Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima and Katsuhiro Tamura : Effects of pressure on the step velocity, two-dimensional nucleation rate and solubility of glucose isomerase crystals, Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology, 44-45, San Diego, Sep. 2008.
54.	Takahisa Fujiwara, Yoshihisa Suzuki and Katsuhiro Tamura : Solubility measurements by in situ observation of the steps of tetragonal lysozyme crystals under high pressure, Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology, 74, San Diego, Sep. 2008.
55.	Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba and Katsuhiro Tamura : X-ray structure analysis of glucose isomerase crystals prepared under high pressure, Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology, 73, San Diego, Sep. 2008.
56.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Colloidal Crystallization on Tilted Flat Substrates by Centrifugation, 2007 MRS Fall Meeting, Boston, Nov. 2007.
57.	Atsushi Mori and Yoshihisa Suzuki : Stacking disorder in hard-sphere crystal under gravity, YITP Workshop 2007 "New Frontiers in Colloidal Physics : A Bridge between Micro- and Macroscopic Concepts in Soft Matter", Vol.89, No.1, 126-127, Kyoto, Jul. 2007.
58.	Satoshi Kawachi, Shin-ichi Nakayama, Yoshio Hara, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of Compressed Rare Gases on Yeast Growth, The Fourth International Conference on High Pressure Bioscience and Biotechnology, Tsukuba, Sep. 2006.
59.	Yoshio Hara, Shin-ichi Nakayama, Satoshi Kawachi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of Compressed Rare Gases on Yeast Growth, The Fourth International Conference on High Pressure Bioscience and Biotechnology, Tsukuba, Sep. 2006.
60.	Tadashi Yamamoto, Yoshihisa Suzuki, Katsuhiro Tamura and : Effects of Antioxidants (α-Lipoic Acid and Vitamin E) on the Phase Transition Temperature of Bilayer Membranes of Phospholipids under Oxygen-Gas Pressure, The Fourth International Conference on High Pressure Bioscience and Biotechnology, Tsukuba, Sep. 2006.
61.	Toshiaki Arao, Yoshihisa Muramoto, Kei Tanigawa, Yoshihisa Suzuki and Katsuhiro Tamura : Sterilization of Sudachi Juice by Hybrid Gas Compression of Oxygen and Nitrogen, The Fourth International Conference on High Pressure Bioscience and Biotechnology, Tsukuba, Sep. 2006.
62.	Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima and Katsuhiro Tamura : High-Pressure Acceleration of the Step Velocities and Two-Dimensional Nucleation Rates on {1 0 1} Faces of Glucose Isomerase Crystals, The Fourth International Conference on High Pressure Bioscience and Biotechnology, Tsukuba, Sep. 2006.
63.	Atsushi Mori, Shin-ichiro Yanagiya and Yoshihisa Suzuki : Succession of stacking fault in hard-sphere crystal under gravity by Monte Carlo simulation, 4th International Symposium on Molecular Thermodynamics and Molecular Simulation, Book of Abstract,, 20, Chiba, May 2006.
64.	Yoshihisa Suzuki, Tsutomu Sawada, Atsushi Mori and Katsuhiro Tamura : Grain-size Control of a Colloidal Crystal by using a Centrifugal Sedimentation Method, 2006 MRS Spring Meeting, San Francisco, Apr. 2006.
65.	Yoshihisa Suzuki, Atsuto Arai and Katsuhiro Tamura : Solubility Measurements of Tetragonal Lysozyme Crystals, International Conference on Advanced Materials Development & Performance, Auckland, Jul. 2005.
66.	Yoshihisa Muramoto, Katsuhiro Tamura, Toshiaki Arao, Takanori Taniwaki and Yoshihisa Suzuki : Sterilization of Sudachi juice with compressed oxygen gas, 3rd International Conference on High Pressure Bioscience and Biotechnology, Rio de Janeiro, Sep. 2004. (Summary) 果汁中の溶存酸素は，さまざまな形で品質劣化の原因となる．本研究は，果汁を窒素ガスで加圧したのち減圧することで，窒素が溶存酸素を同伴する作用を利用して酸素脱気する技術に関するものである．20秒間隔で，加圧と除圧を7回繰り返すことにより，効率的に脱気することができた．30℃，10 MPaで処理することにより，溶存酸素量を0.02 mg/lまで下げることができた．本技術はこれまでにない新しい技術であり，特許の出願につながった.
67.	Hiroyuki Matsuoka, Yoshiteru Suzuki, Hitoshi Iwahashi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Evaluation of biological effects of high-pressure gas using yeast DNA microarray, 3rd International Conference on High Pressure Bioscience and Biotechnology, Rio de Janeiro, Sep. 2004. (Summary) ストレスに対する微生物の応答は，DNAの発現という形で起こるが，ここでは，ガス圧に対する酵母DNAの反応に注目し，そのゲノム解析を行った．供試菌として酵母(Saccharomyces cerevisiae S288C)を用い，窒素ガス圧力下と常圧下で2時間増殖させたものからmRNAを抽出し，それを用いてDNA microarrayで解析を行った．その結果から，生体防御に関する遺伝子の発現が，ガス圧力下で増殖した酵母でより多く見られた．
68.	Takeshi Maruoka, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of pressure on the nucleation of glucose isomerase crystals, 3rd International Conference on High Pressure Bioscience and Biotechnology, Rio de Janeiro, Sep. 2004. (Summary) グルコースイソメラーゼ結晶の核生成頻度に及ぼす圧力効果を測定した．核生成頻度は，その場観察用セル中に過飽和溶液を注入し，顕微鏡で観察し，顕微鏡の分解能以上になった微結晶の数の時間変化をプロットし，その時間変化率から求めた．溶解度の圧力依存性も測定し，それを用いて各圧力における過飽和度を出して，核生成頻度の過飽和度依存性を調べた．同じ過飽和度で比較した時，明らかに高圧力下の方が核生成頻度が大きかったことから，グルコースイソメラーゼ結晶の核生成頻度は加圧によって速度論的に促進されることが明らかになった．
69.	Toshitaka Asai, Yoshihisa Suzuki, Gen Sazaki, Katsuhiro Tamura and Tsutomu Sawada : High-pressure effects on the solubility and growth kinetics of monoclinic lysozyme crystals, 3rd International Conference on High Pressure Bioscience and Biotechnology, Rio de Janeiro, Sep. 2004. (Summary) 単斜晶系リゾチーム結晶の<010>方向の結晶長さの時間変化をR<010>とし，それを0.1, 100MPaの圧力下でその場観察により測定した．成長速度の濃度依存性から溶解度を求めた結果，溶解度は圧力の増加に伴い増加した．0.1, 100MPaにおいて同じ過飽和度下で結晶の成長速度を比較した結果，加圧に伴い，結晶の成長速度は増加した．二次元核成長モデルを用いて解析した結果，その原因は，表面自由エネルギー，活性化エネルギー，そしてステップ上のキンク間距離の加圧に伴う減少にあることがわかった．
70.	Yoshihisa Suzuki, Gen Sazaki, Yoshiki Matsuura, Hironori Hondoh, Haruhiko Sakuraba, Toshihisa Ohshima, Kazuo Nakajima and Katsuhiro Tamura : A Molecular Level Study on the Growth Kinetics of Glucose Isomerase Crystals, 3rd International Conference on High Pressure Bioscience and Biotechnology, Rio de Janeiro, Sep. 2004. (Summary) グルコースイソメラーゼ(GI)斜方晶系結晶(I222)の(101)面の成長速度R101 を，過飽和度σ ( σ = ln(C/Ce), C: 溶液濃度，Ce: 溶解度) の異なる溶液に対する結晶サイズの時間変化をその場観察することによって求めた．σに対してR101 をplot すると，100 MPa におけるR101 の方が明らかに速く，加圧によって速度論的に成長が促進される事が分かった．それを2 次元核成長のbirth-and-spread model でfitting した結果，加圧に伴う二次元核の平均ステップレッジ自由エネルギーαの減少がこの促進の原因である事がわかった．この結果をさらに詳細に考察するために，また，液中AFM(Nanoscope IIIa)を用いて常圧における(101)面の微細構造の観察を行ったところ，結晶の対称性を反映した三角形の微細構造が見られた．また，結晶中の結合の強さを見積もるために，既出の結晶構造解析データ(PDB ID 1MNZ)を用いた結晶中のマクロボンド解析を行った結果，tetramer 形成の強い結合と，tetramer 間の弱い結合に分類される事が分かった．AFMによって得られたステップ高さは，tetramerの存在を裏付けるものであった．
71.	Atsushi Mori, Shin-ichiro Yanagiya, Yoshihisa Suzuki, Tsutomu Sawada, Kensaku Ito and Tetsuo Inoue : Monte Carlo simulation of hard-sphere crystal under gravity, The 57th Divisional Meeting on Colloid and Surface Chemistry with the 2004 Japan-Australia International Symposium, 500, Onoda, Sep. 2004.
72.	Yoshihisa Suzuki, Ning Shi, Tsutomu Sawada and Katsuhiro Tamura : Colloidal Crystallization by Sedimentation, The 1st. NIMS International Conference, Tsukuba, Mar. 2003. (Summary) 直径329nmのポリスチレン粒子を用いて，沈降法によりコロイド結晶を育成した．結晶の成長速度は沈降速度に比例して大きくなったが，分散媒中の塩濃度によって，体積分率が変わるので，それも成長速度に影響を及ぼすことが明らかになった．沈降速度を変化させることで，沈降速度0の場合の結晶化体積分率を外挿によって求めれば，原理的にはコロイド結晶の融解体積分率が得られるはずである．実際に行ってみたところ，同様の系で得られた結晶の融点のデータと比較すると，類似のデータが得られた．
73.	Toshiaki Arao, Yoshihisa Muramoto, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of High-Pressure Gas on the Growth Thermograms of Yeast, Second International Conference on High Pressure Bioscience and Biotechnology, Dortmund, Germany, Sep. 2002. (Summary) 30℃での酵母の培養中に発生した熱量を増殖サーモグラム(代謝熱-培養時間曲線)という形で測定した．増殖サーモグラムは，圧縮ガス環境下における酵母の微生物活性を評価し，培地に溶解したガスの毒性指標として最小阻止圧力(MIP)を決定するために使用した．ガス圧力の増加は，酵母細胞に明らかな阻害活性を導入することが分かった．それは特に空気において顕著であった．その活性の強さ順序は以下の通りである．空気>亜酸化窒素>アルゴン>窒素
74.	Katsuhiro Tamura, Tomohiro Matsumoto and Yoshihisa Suzuki : Effects of Gas Pressure on the Phase Transition Temperature of Liposomal Bilayer Membranes, Second International Conference on High Pressure Bioscience and Biotechnology, Dortmund, Germany, Sep. 2002. (Summary) dimyristoyl phosphatidylcholine (DMPC)とdipalmitoyl phosphatidylcholine (DPPC)で構成されるリン脂質二重膜の主相転移温度(Tm)を様々なガス圧の下で決定した．加圧によるTmの振る舞いは二つのグループに分類された．一つ目のグループでは，Tmは圧力増加とともに直線的に増加したが，その他のグループでは10-20 MPaまでの圧力範囲で減少した．後者のグループの減少は，水中へのガスの大きな溶解度によるものである．特に，N2O(亜酸化窒素)の減少が著しく，これは二分子膜骨格へ溶解するガスの強い影響を示唆している．
75.	Yoshihisa Suzuki, Hiroshi Suto, Tsutomu Sawada and Katsuhiro Tamura : Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals, Second International Conference on High Pressure Bioscience and Biotechnology, Dortmund, Germany, Sep. 2002. (Summary) 斜方晶系リゾチーム結晶の溶解度と成長速度を0.1, 100 MPaで測定した．溶解度は30, 35, 40℃の全ての温度において，加圧に伴い減少した．(010)面の結晶成長速度R(010)を過飽和度σ=ln(C/Ce) (C:溶液濃度 Ce: 溶解度) に対してプロットし，kinetic係数k=R(010)/σを見積もった結果，kは，30, 35, 40℃で加圧に伴い増加した．これは，加圧に伴い成長kineticsも促進されたことを意味する．
76.	Yoshihisa Suzuki, Gen Sazaki, Kalevi Visuri, Katsuhiro Tamura and Kazuo Nakajima : Growth Acceleration of Glucose Isomerase Crystals at High Pressure, Second International Conference on High Pressure Bioscience and Biotechnology, Dortmund, Germany, Sep. 2002. (Summary) グルコースイソメラーゼ結晶の{101}面の成長·溶解速度を0.1, 100 MPaでその場観察により測定した．同じ2.5という過飽和度で比較した結果，成長速度は100MPaにおいて0.1MPaの7.6倍になった．この結果から，加圧によって結晶の成長速度が速度論的に加速されることが世界で初めて明らかになった．二次元核成長モデルを用いて解析した結果，加圧による成長速度の加速の原因は，主に表面自由エネルギーの減少にあることが分かった．
77.	Yoshihisa Suzuki, Gen Sazaki, Kalevi Visuri, Katsuhiro Tamura and Kazuo Nakajima : Remarkable Decrease in the Solubility of Glucose Isomerase under High Pressure, 9th International Conference on the Crystallization of Biological Macromolecules, Jena, Germany, Mar. 2002. (Summary) マイケルソン干渉計を用いてグルコースイソメラーゼ結晶の溶解度を0.1, 100 MPaでその場観察により測定した．ある濃度の溶液が結晶と平衡状態になるための平衡温度を，溶液濃度と圧力を変えて測定した結果，加圧に伴い溶解度が減少した．このことはVisuriらによって報告された加圧による結晶化の促進を支持する．
78.	Yoshihisa Suzuki and Tsutomu Sawada : Growth rate of highly concentrated colloidal crystal, The Thirteenth International Conference on Crystal Growth in Conjunction with The Eleventh International Conference on Vapor Growth and Epitaxy, Kyoto, Aug. 2001. (Summary) 沈降法によるコロイド結晶化を行った．分散媒中のKCl濃度を0.002molkg-1と0.008molkg-1で行ったところ，それぞれφ=0.57, 0.61のコロイド結晶が得られた．これらの結晶は多くの結晶ドメインを含んでいるが，そのうちのいくつかはmmサイズであった．
79.	Hiroshi Matsuo, Seiji Sawamura, Yoshihisa Suzuki and Tetsuya Kawakita : Effect of pressure on the solubility of L-amino acids in water at 298.15 K, 18th International Conference of High Pressure Science and Technology & 11th High Pressure Conference of China, Beijing, Jul. 2001. (Summary) 高圧力下におけるglycine, L-alanine, L-valine, L-leucine, and L-isoleucineの水に対する溶解度を298.15Kで400MPaの圧力まで測定した．その結果，アミノ酸の種類によって様々な結果が明らかになった．glycineは加圧にともない溶解度が減少したのに対し，L-alanineの溶解度は逆に増加した．L-valineとL-isoleucine は100MPa付近に溶解度の極大があり，L-leucineは200MPa付近に固相相転移が起こっていることが明らかになった．
80.	Yoshihisa Suzuki, Satoru Miyashita, Tsutomu Sawada, Gen Sazaki, Toshitaka Nakada, Hiroshi Komatsu, Toshiaki Arao and Katsuhiro Tamura : Effect of pressure on protein crystallization, The satellite meeting of The First International Conference on High Pressure Bioscience and Biotechnology, Kyoto, Nov. 2000. (Summary) 0.1, 50, 100 MPaで正方晶系リゾチーム結晶の面成長速度をその場観察した．高圧力下での溶解度からもとめた高圧力下での過飽和度に対する成長速度を測定したところ，同じ過飽和度でも，両方の結晶面とも加圧に伴い成長速度が減少した．二次元核成長の多核成長モデルを使って成長kineticsにおよぼす圧力効果について解析したところ，加圧に伴う表面自由エネルギーの増加が成長速度の減少の原因であることが明らかになった．また，100 MPa1時間の加圧を施した5mg/mlのリゾチームのモル熱変性エンタルピーを示差走査型熱量計を用いて測定した結果，未加圧の試料と変わらなかったので，本研究における100 MPaまでの圧力による不可逆変性の影響は無視出来ることがわかった．
81.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Hiroshi Komatsu, Toshiaki Arao and Katsuhiro Tamura : In situ measurements of the solubility of protein crystals under high pressure, The First International Conference on High Pressure Bioscience and Biotechnology, Kyoto, Nov. 2000. (Summary) リゾチーム正方晶系結晶の溶解度に及ぼす圧力効果をマッハツェンダー干渉計と，高圧光学セルを用いてその場観察により測定した．結晶の平衡化過程を，過飽和状態·未飽和状態の両方から正確にかつ連続的に測定した．未飽和状態から到達した濃度を平衡濃度としたところ加圧により溶解度が増加することが明らかになった．また，この実験の条件でのタンパク質の圧力変性の有無をモルエンタルピーの測定から確認した結果，実験中の変性はないという事が明らかになった．
82.	Kazuhiro Hisada, Yoshihisa Suzuki and Katsuhiro Tamura : Thermotolerance and Barotolerance of Alcohol-Shocked Yeast, The Ist International Conference on High Pressure Bioscience and Biotechnology, Kyoto, Nov. 2000. (Summary) 様々な濃度のアルコールショック処理によるストレス耐性の獲得を酵母Saccharomyces cerevisiaeについて，8種類のアルコールを用いて研究した．アルコールショック処理は酵母に高温や高圧力といったストレスへの耐性を導入した．これらのストレスに対する最大耐性を導く最適アルコールショック濃度はアルコールの物性に依存した．最適濃度はアルコール分子の炭素数に逆比例した．さらに，アルコール分子の水酸基の位置はストレス耐性の強さに影響を及ぼした．また，ストレス物質や酵母の成長阻害物質としてのアルコールの強さを評価するためにマイクロカロリメトリーを行った．酵母の増殖に伴う発熱曲線からアルコールの最小阻止濃度(MIC)を決定し，アルコールショックの最適アルコール濃度との関係を考察した．
83.	Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : Effects of Saccharide in Medium on Stress Tolerance of Yeast, The Ist International Conference on High Pressure Bioscience and Biotechnology, Kyoto, Nov. 2000. (Summary) 8種類のアルコールを用いてアルコールショックを加えた酵母菌のストレス耐性に関する研究．アルコールショック処理により酵母に高温，高圧力や薬物に対するストレス耐性が誘導された．最大耐性を誘導する最適アルコールショック濃度は，アルコールの物性に依存した．この最適濃度はアルコール分子の炭素数に逆比例した．さらに，アルコール分子の水酸基の位置はストレス耐性の強さに影響を及ぼすことが分かった．また，マイクロカロリメトリーにより，酵母の増殖に伴う発熱曲線からアルコールの最小阻止濃度(MIC)を決定し，アルコールショックの最適アルコール濃度との関係を考察している
84.	Yoshihisa Suzuki, Satoru Miyashita, Tsutomu Sawada, Gen Sazaki, Toshitaka Nakada, Hiroshi Komatsu, Toshiaki Arao and Katsuhiro Tamura : Effects of pressure on growth kinetics of protein crystals, The First International Conference on High Pressure Bioscience and Biotechnology, Kyoto, Nov. 2000. (Summary) 高圧力下で正方晶系ニワトリ卵白リゾチーム結晶の{110}, {101}面の面成長速度をその場観察した．高圧力下での溶解度から高圧力下での過飽和度を決定し，過飽和度に対する成長速度のプロットを作成したところ同じ過飽和度でも，両方の結晶面とも加圧に伴い成長速度が減少した．二次元核成長の多核成長モデルを使って解析したところ，加圧に伴う平均レッジ表面自由エネルギーの増加によって，成長速度の減少が説明できた．また，100 MPa1時間の加圧での5mg/mlのリゾチームのモル熱変性エンタルピーを示差走査型熱量計を用いて測定した結果，未加圧の試料と変わらなかった．
85.	Tsutomu Sawada and Yoshihisa Suzuki : Flow-induced modification of texture of colloidal crystal, International Conference on Colloid and Surface Science, Tokyo, Nov. 2000. (Summary) 脱塩によって作成したポリスチレン微粒子のコロイド結晶を厚さ0.1 mm横幅1cmのキャピラリー空間の中で流すことで，結晶にずり応力を与えて融解させ，急激にストップすることで，cmオーダーの巨大なコロイド単結晶を育成することに成功した．これまでにあった度の方法よりも巨大な単結晶をわずか数秒で育成出来た．
86.	Yoshihisa Suzuki and Tsutomu Sawada : Coagulation condition of highly concentrated latex suspension, International Conference on Colloid and Surface Science, Tokyo, Nov. 2000. (Summary) ポリスチレン微粒子の水分散系において，コロイド結晶化·凝集が起こる体積分率の塩濃度依存性を測定した．その結果，コロイド結晶の融解体積分率は塩濃度の増加に伴い増加し，0.01 molkg-1程度で最大値(φ=0.6程度)を取り，それ以上では凝集が開始することが分かった．また，凝集開始の体積分率は，塩濃度の増加とともに減少したが，その依存性は希薄分散系における凝集挙動と異なることが分かった．濃厚分散系では粒子間の斥力ポテンシャルの重なりあいによって，希薄系とは異なる相挙動を示したものと考えられる．
87.	Gen Sazaki, Yukiko Nagatoshi, Yoshihisa Suzuki, Satoru Miyashita, Toru Ujihara, Kazuo Nakajima and Hiroshi Komatsu : Effects of hydrostatic pressure on the growth kinetics of tetragonal and orthorhombic lysozyme crystals, Recent Advances in Macromolecular Crystallization, San Diego, Aug. 1999. (Summary) 溶解度の圧力依存性では，ニワトリ卵白リゾチームの正方晶系結晶では加圧に伴う増加が見られた．それに反して斜方晶系結晶では加圧に伴い溶解度の減少が見られた．そこで，速度論的な部分に関してどのような圧力依存性があるかを，それぞれの常圧下，高圧下における過飽和度に対する結晶成長速度の依存性を求めたところ，両晶系とも加圧に伴い速度論的に成長速度が抑制されていることが明らかになった．その原因については，正方晶系結晶では表面自由エネルギーの増加，斜方晶系については，溶質の結晶取込み過程における活性化エネルギーの増加の効果が強いということが明らかになった．
88.	Yoshihisa Suzuki, Hiroshi Matsuo, Tomohiro Takagi, Osamu Fujimoto and Seiji Sawamura : Effect of Pressure on the Solubilities of Glycine, L-Glutamic Acid and Monosodium L-Glutamate Monohydrate in Water, The eighth International Symposium on Solubility Phenomena, Niigata, Aug. 1998. (Summary) アミノ酸の水に対する溶解度の圧力依存性の測定の第一歩として，グルタミン酸ナトリウム一水和物の水に対する溶解度を0.10-300 MPaの圧力範囲で298.15 Kで測定した．重量モル濃度で示される溶解度msは加圧に伴い増加した．その圧力係数dlnms/dp=(2.0±0.1)X10^(-10) Pa^(-1)は，密度測定によって求められた部分モル体積と活量係数と結晶のモル体積から熱力学的に求められた値(2.1±0.2)X10^(-10) Pa^(-1)と一致した．また，グリシンは加圧により溶解度が減少し，グルタミン酸は一度増加した溶解度が300MPa異常で減少した．
89.	Yukiko Nagatoshi, Gen Sazaki, Yoshihisa Suzuki, Satoru Miyashita, Toshitaka Nakada, Duen Stephen Durbin and Hiroshi Komatsu : Solubility measurement of lysozyme under high pressure by two-beam interferometry, The 7th International Conference on the Crystallization of Biological Macromolecules, Granada, May 1998. (Summary) ニ光束干渉計を用いて，結晶周囲の濃度場を観察し，温度の変化に対する結晶の成長·溶解に伴う干渉縞の変化から，ある濃度の溶液における溶解温度を決定した．この作業を様々な溶液濃度，圧力で行うことで，溶解度の温度圧力依存性を測定した．正方晶系リゾチームに関しては0.1, 50, 100 MPa，斜方晶系リゾチームに関しては，0.1, 100 MPaで温度の関数として溶解度を測定した．その結果，加圧に伴い，正方晶系の方は溶解度が増加し，それに対して斜方晶系では減少した．双方とも溶解エンタルピーは加圧に伴い減少した．
90.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita and Hiroshi Komatsu : Effect of Pressure on Crystal Growth Kinetics of Lysozyme, The 7th International Conference on the Crystallization of Biological Macromolecules, Granada, May 1998. (Summary) 正方晶系リゾチーム結晶の成長kineticsを高圧力下で，光学高圧セルを用いたその場測定により研究した．二次元核成長のbirth and spread modelでfittingしたところ，平均ステップレッジ表面自由エネルギーが加圧とともに増加することが，成長速度の減少につながるということが明らかになった．
91.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita and Hiroshi Komatsu : Effect of pressure on crystallization of lysozyme, RU-UBC Joint Symposium "Proteins and Biosolutions", Kusatsu, Dec. 1997. (Summary) ニワトリ卵白リゾチームの結晶成長に及ぼす圧力効果を，核生成頻度，結晶成長速度のその場観察と，溶解度の圧力依存性の測定より明らかにした．溶解度の圧力依存性を結晶の溶解·成長に伴う上澄み溶液の濃度の時間変化を用いて求め，それを元にした各圧力での過飽和度によって速度論的な解析を行った結果，高圧力下でリゾチーム正方晶系結晶の表面自由エネルギーが増加することが明らかになった．また，タンパク質は，アミノ酸によって構成されているので，水中におけるタンパク質の挙動は，構成するアミノ酸残基が水中でどのような性質を示すかというところに帰されるので，アミノ酸の溶解度に及ぼす圧力効果から議論出来る可能性を示した．
92.	Masanori Matsuki, Seiji Sawamura, Yoshihisa Suzuki and Yoshihiro Taniguchi : Effect of pressure on the solubility of lysozyme in water, The satellite meeting of AIRAPT-16 & HPCJ-38 "Structure and Function of Biological Systems under Extreme Conditions", Kyoto, Aug. 1997. (Summary) タンパク質の溶解度の圧力依存性は測定手法によって異なっている．その原因を明らかにするために，二種類の測定法によって求められる溶解度の比較を行った．一つは種結晶の周囲の溶液を入れ替え，結晶成長速度のその場観察を行って，成長速度ゼロの時の溶液濃度を求める方法．もう一つは，長時間高圧容器内に結晶と溶液の共存したセルを多数入れ，その上澄み溶液濃度の時間変化から溶解度を求めた方法である．結果として，二つの方法の間で違いがあった．その原因としては，二番目の方法ではまだ平衡状態に達していない段階で溶解度を決定したためと考えられた．
93.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita and Hiroshi Komatsu : Effect of pressure on protein crystal growth, The satellite meeting of AIRAPT-16 & HPCJ-38 "Structure and Function of Biological Systems under Extreme Conditions", Kyoto, Aug. 1997. (Summary) タンパク質結晶化に及ぼす圧力効果を，結晶成長過程のその場観察と，二光束干渉法を用いた平衡化過程のその場観察から明らかにした．結晶の核生成頻度と成長速度が加圧によって減少したが，その機構は，溶解度の圧力依存性を明らかにしなければ特定できない．結晶の溶解·成長に伴う上澄み溶液の濃度の時間変化を用いて溶解度を求め，それを元にした各圧力での過飽和度によって速度論的な解析を行った結果，高圧力下でリゾチーム正方晶系結晶の表面自由エネルギーが増加することが明らかになった．
94.	Yoshihisa Suzuki, Hiroshi Komatsu, Tsutomu Sawada and Satoru Miyashita : Effect of pressure on protein crystal growth, Recent Advances in Macromolecular Crystallization, Le Bischenberg, Jun. 1996. (Summary) ダイヤモンドアンビルセルを用いた，リゾチーム正方晶系結晶の核生成頻度，結晶成長速度の圧力依存性と，溶解度のその場測定法について研究した．圧力は温度と異なり，系内に瞬時に均一に伝播するため，結晶化のコントロールをする上で有効なパラメタである．特に顕微鏡下で高倍率のその場観察が出来，容易な操作で加圧減圧が自由に行えるよう設計したダイヤモンドアンビルを用いることで，特に核生成をコントロール出来ることが明らかになった．
95.	Yoshihisa Suzuki, Tsutomu Sawada, Satoru Miyashita and Hiroshi Komatsu : Pressure dependence of the solubility of hen egg white lysozyme measured by in situ observation, The 6th International Conference on Crystallization of Biological Macromolecules, Hiroshima, Nov. 1995. (Summary) キャピラリーチューブ二本を使用し，一方に緩衝溶液を入れ，もう一方に結晶と共存するタンパク質溶液を入れて，結晶の成長溶解に伴う濃度変化をマッハツェンダー型二光束干渉計を用いて高圧力下で正確に測定することが出来，濃度の時間変化から，溶解度を測定できた．その結果，高圧力下でリゾチーム正方晶系結晶の溶解度が増加することを明らかにした．
96.	Kazuo Kurihara, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Yoshihisa Suzuki and Hiroshi Komatsu : Interferometric study on crystal growth of tetragonal lysozyme crystal, The 11th International Conference on Crystal Growth, Hague, Jun. 1995. (Summary) リゾチーム正方晶系結晶の結晶成長速度を速度論的に解析する上で，結晶周囲の濃度場から結晶表面の濃度を正確に求め，表面過飽和度に対する結晶成長速度の解析を世界で初めて行った．また，濃度勾配と結晶成長速度の関係から結晶表面でのリゾチーム分子の拡散定数を求めた．

Proceeding of Domestic Conference:

1.	Yoshihisa Suzuki and Keigo Kishida : 引力系コロイド結晶の臨界核生成付近のクラスタ形成のその場観察, 日本物理学会2024年春季大会, Mar. 2024.
2.	土塔悟司, Shin-ichiro Yanagiya and Yoshihisa Suzuki : 単斜晶系リゾチーム結晶のステップ前進速度の異方性と結晶中の分子間結合の異方性との関係, 第52回結晶成長国内会議, Dec. 2023.
3.	Yoshihisa Suzuki and Keigo Kisida : 板状引力系コロイド結晶の臨界核生成プロセスのその場観察, 第52回結晶成長国内会議, Dec. 2023.
4.	Ryusei Fukunaga, Masahide Sato and Yoshihisa Suzuki : 密度マッチング条件下における引力系コロイド結晶の3次元核生成プロセスのその場観察, 第52回結晶成長国内会議, Dec. 2023.
5.	Yoshihisa Suzuki : リゾチーム分子構造の塩濃度・温度変化, 日本物理学会第78回年次大会, Sep. 2023.
6.	Yoshihisa Suzuki, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Masae Nagai, Koji Inaka, Shin-ichiro Yanagiya, Takehiko Sone, Shingo Wachi, Satoshi Kawaguchi, Yasutomo Arai and Katsuo Tsukamoto : 高精製グルコースイソメラーゼ結晶のステップ前進速度に及ぼす重力の影響, 日本物理学会2023年春季大会, Mar. 2023.
7.	Yoshihisa Suzuki and Shiori Fujiwara : 結晶化条件の変化によるリゾチーム分子立体構造の変化, 第51回結晶成長国内会議, Nov. 2022.
8.	Shiori Fujiwara and Yoshihisa Suzuki : 蒸発による沈殿剤フリーのタンパク質の結晶化・結晶構造解析, 第51回結晶成長国内会議, Nov. 2022.
9.	Yutaka Tsuboi, Masahiro Kanno, Shigeki Matsuo and Yoshihisa Suzuki : 高速度カメラを用いた枯渇凝集引力系コロイド結晶成長界面における粒子の2次元拡散プロセスの解明, 第51回結晶成長国内会議, Nov. 2022.
10.	Masahiro Kanno, Yutaka Tsuboi, Shigeki Matsuo and Yoshihisa Suzuki : 枯渇凝集引力系コロイド結晶のキンク前進速度, 第51回結晶成長国内会議, Nov. 2022.
11.	片山遥登, Momoka Morishita, Shin-ichiro Yanagiya and Yoshihisa Suzuki : ニワトリ卵白リゾチーム共存下でのグルコースイソメラーゼ結晶のらせん成長丘におけるステップ前進速度の異方性, 第51回結晶成長国内会議, Nov. 2022.
12.	SHUMPEI Marukawa and Yoshihisa Suzuki : 沈殿剤フリーの超濃厚溶液中での大型GI結晶の高速育成, 第51回結晶成長国内会議, Nov. 2022.
13.	Yoshihisa Suzuki, Katsuo Tsukamoto, Gen Sazaki, Seijiro Fukuyama, Taro Shimaoka, Masae Nagai, Takehiko Sone, Shingo Wachi, Yasutomo Arai, Izumi Yoshizaki, Masahiro Kanno, Yutaka Tsuboi, Shin-ichiro Yanagiya and Shigeki Matsuo : その場観察による結晶成長機構の解明, 日本セラミックス協会第35回秋季シンポジウム, Sep. 2022.
14.	Yoshihisa Suzuki, Naoto Ikemitsu and Shin-ichiro Yanagiya : タンパク質の高速・高品質結晶化, 日本物理学会2022年秋季大会, Sep. 2022.
15.	Yoshihisa Suzuki : 乾燥によるタンパク質結晶化, 日本物理学会第77回年次大会, Mar. 2022.
16.	Naoto Ikemitsu and Yoshihisa Suzuki : 超濃厚溶液中でのグルコースイソメラーゼ結晶の成長, 日本物理学会第77回年次大会, Mar. 2022.
17.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, Taro Shimaoka, Seijiro Fukuyama, Yasutomo Arai, Takehiko Sone, Shingo Wachi, Masae Nagai, Takahisa Fujiwara, Ai Ninomiya and Satoshi Kawaguchi : Two-beam interferometry of growth interfaces of protein crystals under microgravity conditions, レーザー学会学術講演会第42回年次大会, Jan. 2022.
18.	Momoka Morishita, Shin-ichiro Yanagiya and Yoshihisa Suzuki : グルコースイソメラーゼ結晶のステップ前進速度に対するニワトリ卵白リゾチームの不純物効果, 第50回結晶成長国内会議, Oct. 2021.
19.	Shiori Fujiwara, Takashi Sakai and Yoshihisa Suzuki : 蒸発によるタンパク質の結晶化および結晶構造解析, 第50回結晶成長国内会議, Oct. 2021.
20.	Yutaka Tsuboi, Shigeki Matsuo and Yoshihisa Suzuki : 高速度カメラを用いた枯渇凝集引力系コロイド結晶表面吸着粒子の2次元拡散の活性化エネルギーの測定, 第50回結晶成長国内会議, Oct. 2021.
21.	Masahiro Kanno, Yutaka Tsuboi, Shigeki Matsuo and Yoshihisa Suzuki : 引力系コロイド結晶における粒子のキンク取り込み過程の活性化エネルギー, 第50回結晶成長国内会議, Oct. 2021.
22.	Yoshihisa Suzuki : 超濃厚溶液からのタンパク質結晶化, 第50回結晶成長国内会議, Oct. 2021.
23.	Yoshihisa Suzuki : 分子クラウディング様条件下におけるタンパク質の結晶化および結晶構造解析, 日本物理学会第76回年次大会, Mar. 2021.
24.	Yoshihisa Suzuki and Takashi Sakai : 生理食塩水中におけるタンパク質の結晶構造, 第49回結晶成長国内会議, Nov. 2020.
25.	Naoto Ikemitsu and Yoshihisa Suzuki : 液液相分離後の濃厚相で核生成するグルコースイソメラーゼ結晶のその場観察, 第49回結晶成長国内会議, Nov. 2020.
26.	Momoka Morishita, Shin-ichiro Yanagiya and Yoshihisa Suzuki : グルコースイソメラーゼ結晶のステップ前進速度に対する不純物効果, 第49回結晶成長国内会議, Nov. 2020.
27.	Yoshihisa Suzuki, Katsuo Tsukamoto, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Masae Nagai, Hitoshi Miura, Takahisa Fujiwara, Koji Inaka, Takehiko Sone, Shingo Wachi, Izumi Yoshizaki and Yasutomo Arai : タンパク質結晶の成長と不純物効果, 第49回結晶成長国内会議, Nov. 2020. (Keyword) glucose isomerase / microgravity / growth rates
28.	Yoshihisa Suzuki, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Masae Nagai, Koji Inaka, Takehiko Sone, Shingo Wachi, Katsuo Tsukamoto and Yasutomo Arai : 微小重力下におけるグルコースイソメラーゼ結晶の成長速度, 日本物理学会年次大会, Mar. 2020. (Keyword) glucose isomerase / microgravity / growth rates
29.	Yoshihisa Suzuki, Hideaki Tsuge, Hironori Hondoh, Yusuke Kato, Nobuo Maita, Yuta Uehara and Koji Inaka : 結晶化条件によって変化するタンパク質の分子構造, 第48回結晶成長国内会議, Nov. 2019.
30.	Hitomi Minamizono, Katsuo Tsukamoto, Yuko Inatomi, Kazuma Tsuchiya and Yoshihisa Suzuki : 引力系ポリスチレン粒子性結晶の成長界面における粒子の表面拡散に及ぼす重力の影響, 第48回結晶成長国内会議, Oct. 2019.
31.	Yoshihisa Suzuki, Takahisa Fujiwara, Hideaki Tsuge, Hironori Hondoh, Yusuke Kato, Yuta Uehara, Nobuo Maita, Kohei Hosokawa and Shoko Ueta : 濃縮のみによるタンパク質結晶化と結晶構造解析, 日本物理学会年次大会, Mar. 2019. (Keyword) precipitant-free / lysozyme / centrifugal concentration / synchrotron x-ray crystallography
32.	Yoshihisa Suzuki, Akiho Ikeuchi, Koichiro Taoka, Tomoki Yabutani, Noriyoshi Nishida, Masae Nagai and Seijiro Fukuyama : タンパク質結晶成長プロセスの長期間継続観察用セルの開発と溶液媒介相転移の観察, 第47回結晶成長国内会議, Nov. 2018.
33.	Ai Ninomiya, Yoshihisa Suzuki, Takahisa Fujiwara, Shin-ichiro Yanagiya, Yasutomo Arai and Masae Nagai : グルコースイソメラーゼ結晶のスパイラル成長丘におけるステップのその場観察, 第47回結晶成長国内会議, Nov. 2018.
34.	Yoshihisa Suzuki, Takahisa Fujiwara, Hideaki Tsuge, Hironori Hondoh, Yusuke Kato, Yuta Uehara, Nobuo Maita, Kohei Hosokawa and Shoko Ueta : 濃縮するだけで実現するタンパク質結晶化, 第47回結晶成長国内会議, Nov. 2018. (Keyword) precipitant-free / lysozyme / centrifugal concentration / synchrotron x-ray crystallography
35.	Kazuma Tsuchiya, Yoshihisa Suzuki, Koji Inaka, Hiroaki Tanaka and Masae Nagai : 常温におけるタンパク質結晶の放射光X線構造解析, 第47回結晶成長国内会議, Nov. 2018. (Keyword) グルコースイソメラーゼ / synchrotron radiation / 常温 / X-ray structure analysis
36.	Yoshihisa Suzuki, Yoshiaki Hattori, 野澤純, 宇田聡, 豊玉彰子 and 山中淳平 : 枯渇凝集コロイド結晶の成長界面における粒子の吸着，脱着，表面拡散，格子欠陥形成およびキンク取り込み過程, 第46回結晶成長国内会議, Nov. 2017. (Keyword) 枯渇凝集 / colloidal crystal / adsorption / 脱着 / 表面拡散 / 格子欠陥 / キンク取り込み
37.	Kazuma Tsuchiya, Takahisa Fujiwara, Ai Ninomiya, 伊中浩治, 田仲広明, Masahide Sato and Yoshihisa Suzuki : 放射光を用いたグルコースイソメラーゼ結晶の常温での構造解析, 第46回結晶成長国内会議, Nov. 2017. (Keyword) グルコースイソメラーゼ / synchrotron radiation / 常温 / X-ray structure analysis
38.	Takahisa Fujiwara, Shin-ichiro Yanagiya, Masaru Tachibana, Tomoki Yabutani, Masahide Sato and Yoshihisa Suzuki : 異種タンパク質存在下で結晶化させたグルコースイソメラーゼ結晶のステップ前進速度, 第46回結晶成長国内会議, Nov. 2017. (Keyword) グルコースイソメラーゼ / crystallization / ステップ前進速度
39.	Yoshihisa Suzuki, Haruhiko Koizumi, Masaru Tachibana, 田仲広明 and 伊中浩治 : 放射光を用いて得られたロッキングカーブと振動写真を併用したタンパク質結晶の品質評価, 第46回結晶成長国内会議, Nov. 2017. (Keyword) synchrotron radiation / ロッキングカーブ / 振動写真 / タンパク質結晶 / 品質評価
40.	Atsushi Mori, Masahide Sato and Yoshihisa Suzuki : Monte Carlo simulation of defect disappearance in colloidal crystal under gravity, 化学工学会第49回秋季大会, Sep. 2017.
41.	鈴木凌, 岸健晴, Haruhiko Koizumi, Katsuo Tsukamoto, 荒井康智, 福山誠二郎, Yoshihisa Suzuki, 小島謙一 and Masaru Tachibana : グルコースイソメラーゼ結晶の成長転位の同定, 日本物理学会第71回年次大会, Mar. 2016.
42.	Yoshihisa Suzuki and Nobuo Maita : グルコースイソメラーゼの沈澱剤フリー結晶化と結晶構造解析, 日本物理学会第71回年次大会, Mar. 2016.
43.	Yoshihisa Suzuki, Katsuo Tsukamoto, Takahisa Fujiwara and Shin-ichiro Yanagiya : 地上対流抑制実験におけるタンパク質結晶のステップ前進速度, 第30回宇宙環境利用シンポジウム, Jan. 2016.
44.	Daido Nakahashi, Shin-ichiro Yanagiya, Takahisa Fujiwara, Masaomi Matsumoto, Tomohiro Shiomoto and Yoshihisa Suzuki : リゾチーム存在下におけるグルコースイソメラーゼ結晶のステップ前進速度と二次元核生成頻度, 第45回結晶成長国内会議, Oct. 2015.
45.	Tomohiro Shiomoto, Shin-ichiro Yanagiya, Takahisa Fujiwara, Masaomi Matsumoto, Daido Nakahashi and Yoshihisa Suzuki : グルコースイソメラーゼ結晶の分子取り込み過程における活性化エネルギー, 第45回結晶成長国内会議, Oct. 2015.
46.	Takahisa Fujiwara, Yoshihisa Suzuki, Masahide Sato, Gen Sazaki and Hiroyasu Katsuno : 異種タンパク質存在下で結晶化させたグルコースイソメラーゼ結晶の成長速度, 第45回結晶成長国内会議, Oct. 2015.
47.	鈴木凌, 岸健晴, Haruhiko Koizumi, Katsuo Tsukamoto, 荒井康智, 福山誠二郎, Yoshihisa Suzuki, 小島謙一 and Masaru Tachibana : グルコースイソメラーゼ結晶の転位の運動と塑性変形, 第45回結晶成長国内会議, Oct. 2015.
48.	Tetsuya Toyooka, Masahide Sato and Yoshihisa Suzuki : 遠心力による粒子沈降方向に垂直なセル壁面へのコロイド結晶の配向の変化, 第45回結晶成長国内会議, Oct. 2015.
49.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, Hitoshi Miura and Takahisa Fujiwara : 宇宙空間での正方晶系リゾチーム結晶の成長プロセスに及ぼす不純物効果, 第45回結晶成長国内会議, Oct. 2015.
50.	Yoshihisa Suzuki : 遠心濃縮によるグルコースイソメラーゼの結晶化, 第45回結晶成長国内会議, Oct. 2015.
51.	鈴木凌, 岸健晴, Haruhiko Koizumi, Katsuo Tsukamoto, 荒井康智, 福山誠二郎, Yoshihisa Suzuki, 小島謙一 and Masaru Tachibana : 放射光単色X線トポグラフィによるグルコースイソメラーゼ結晶中の転位発生の観察, 日本物理学会2015年秋季大会, Sep. 2015.
52.	Katsuo Tsukamoto, 三浦均, Yoshihisa Suzuki, 村山健太, Takahisa Fujiwara and Izumi Yoshizaki : 地上とKiboで精密測定されたリゾチーム結晶成長速度, 第29回宇宙環境利用シンポジウム, Jan. 2015.
53.	Yoshihisa Suzuki, Katsuo Tsukamoto, Izumi Yoshizaki, 福山誠二郎, Takahisa Fujiwara, Shin-ichiro Yanagiya, Masaru Tachibana and Haruhiko Koizumi : 溶液相の状態変化によるタンパク質結晶の界面成長kineticsおよび界面morphologyの変化, 第29回宇宙環境利用シンポジウム, Jan. 2015.
54.	植原悠太, Yusuke Kato, Shoko Ueta and Yoshihisa Suzuki : 高圧力下におけるタンパク質結晶のその場X線結晶構造解析, 第55回高圧討論会, Nov. 2014.
55.	藤根守, Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki : ブラウン動力学法を用いた容器形状と外力による結晶構造制御, 第44回結晶成長国内会議, Nov. 2014.
56.	岸健晴, 鈴木凌, Masaru Tachibana, Haruhiko Koizumi, 若生啓, 小島謙一, Izumi Yoshizaki, 福山誠二郎, Yoshihisa Suzuki and Katsuo Tsukamoto : X線トポグラフィによるグルコースイソメラーゼ結晶の完全性評価, 第44回結晶成長国内会議, Nov. 2014.
57.	Takahisa Fujiwara, Yoshihisa Suzuki, Izumi Yoshizaki, Katsuo Tsukamoto, 村山健太, 福山誠二郎, Masaru Tachibana and 三浦均 : 長期宇宙実験での蒸発によるリゾチーム溶液の過飽和度変化への対応方法, 第44回結晶成長国内会議, Nov. 2014.
58.	Takahisa Fujiwara, Yoshihisa Suzuki, Masahide Sato, Gen Sazaki, Hiroyasu Katsuno, Shin-ichiro Yanagiya and 加藤有介 : タンパク質の結晶化における異種タンパク質の影響, 第44回結晶成長国内会議, Nov. 2014.
59.	Yoshihisa Suzuki, Shin-ichiro Yanagiya, Katsuo Tsukamoto, Izumi Yoshizaki, Masaru Tachibana, Takahisa Fujiwara and 加藤有介 : 環境相の変化に伴うリゾチーム結晶の界面モルフォロジーの変化, 第44回結晶成長国内会議, Nov. 2014.
60.	Takahito Suzuta, Yohei Yamada, Tomoki Yabutani, Toshio Takayanagi and Yoshihisa Suzuki : トラックエッチドメンブレンフィルター孔でのリゾチーム結晶成長, 日本分析化学会第63年会, Sep. 2014.
61.	Yohei Yamada, Takahito Suzuta, Tomoki Yabutani, Yoshihisa Suzuki, 永瀬丈嗣, 保田英洋 and Toshio Takayanagi : フェリチンの結晶を鋳型とした規則構造体の合成, 日本分析化学会第63年会, Sep. 2014.
62.	Takahisa Fujiwara, Yoshihisa Suzuki, Masahide Sato, Gen Sazaki and Hiroyasu Katsuno : リゾチーム存在下でのグルコースイソメラーゼ結晶の平衡温度, 第43回結晶成長国内会議, Nov. 2013.
63.	Yoshihisa Suzuki, Hideaki Tsuge, Shoko Ueta and Kohei Hosokawa : 沈澱剤フリー高濃度リゾチーム溶液からの結晶成長, 第43回結晶成長国内会議, Nov. 2013.
64.	Takahito Suzuta, Yohei Yamada, Tomoki Yabutani, Toshio Takayanagi and Yoshihisa Suzuki : リゾチーム単結晶への金属酸化物前駆体の含浸, 第3回CSJ化学フェスタ2013, Oct. 2013.
65.	Shingo Azuma, Atsushi Mori, Yoshihisa Suzuki, Masanobu Haraguchi, Shuichi Hashimoto and Tsutomu Sawada : Adhesion of metal nano particles on gel-immobilized colloidal crystal aiming at hybrid effect between photonic band and plasmonics, 日本物理学会2013年秋季大会, Sep. 2013.
66.	Atsushi Mori and Yoshihisa Suzuki : Experimental application of nucleation theorem reconsidered: nucleation work for critical nucleus in terms of the true supercooling Δμ, 日本物理学会2013年秋季大会, Sep. 2013.
67.	Atsushi Mori and Yoshihisa Suzuki : Identification of defects in hard-sphere crystal grown on square pattern under gravity, 日本物理学会2013年秋季大会, Sep. 2013.
68.	Takahito Suzuta, Yohei Yamada, Tomoki Yabutani, Toshio Takayanagi and Yoshihisa Suzuki : Penetration of precursors of metallic oxide into lysozyme crystals, 第19回中国四国支部分析化学若手セミナー, Sep. 2013.
69.	Atsushi Mori, Shingo Azuma, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, Tsutomu Sawada, Shuichi Hashimoto and Masanobu Haraguchi : フォトニックバンドとプラズモニクスとのハイブリッド効果による強力な電場増強を起こすナノ構造の作成, 第42回結晶成長国内会議, Nov. 2012.
70.	Takahisa Fujiwara, Yoshihisa Suzuki, Masahide Sato, Gen Sazaki and Hiroyasu Katsuno : グルコースイソメラーゼ結晶の成長過程に及ぼすリゾチームの不純物効果, 第42回結晶成長国内会議, Nov. 2012.
71.	中尾恭兵, Yoshihisa Suzuki, Takahisa Fujiwara, Masahide Sato, Gen Sazaki and Hiroyasu Katsuno : リゾチーム結晶成長に及ぼすグルコースイソメラーゼの不純物効果, 第42回結晶成長国内会議, Nov. 2012.
72.	塩澤宏章 and Yoshihisa Suzuki : タンパク質結晶の乾燥, 第42回結晶成長国内会議, Nov. 2012.
73.	細川晃平 and Yoshihisa Suzuki : 遠心濃縮によるリゾチームの結晶化と溶解度測定, 第42回結晶成長国内会議, Nov. 2012.
74.	Yoshihisa Suzuki : 沈澱剤フリーで結晶化したリゾチーム結晶の構造解析, 第42回結晶成長国内会議, Nov. 2012.
75.	伊藤大祐 and Yoshihisa Suzuki : 撥水性基板を用いたコロイド結晶のグレインサイズ制御, 第42回結晶成長国内会議, Nov. 2012.
76.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : コロイドエピタキシーにおける重力テンパリングの可能性, 第42回結晶成長国内会議, Nov. 2012.
77.	Shingo Azuma, Keisuke Ohkubo, Atsushi Mori, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, 澤田勉, Shuichi Hashimoto and Masanobu Haraguchi : フォトニックバンドとプラズモニクスのハイブリッドによる強力な電場増強効果を起こすナノ構造の作製, 日本光学会年次学術講演会Optics & Photonics Japan 2012, Oct. 2012.
78.	Atsushi Mori, Yoshihisa Suzuki, Shigeki Matsuo and Kensaku Ito : コロイドエピタキシーにおける重力テンパリングによる欠陥低減化のモンテカルロ・シミュレーション, 第2回ソフトマター研究会, Sep. 2012.
79.	Atsushi Mori, Shingo Azuma, Keisuke Ohkubo, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, Tsutomu Sawada, Shuichi Hashimoto and Masanobu Haraguchi : ゲル固定化コロイド結晶を用いたフォトニックバンド/プラスモニクスハイブリッド効果による強力な電場増強効果を示すナノ構造の作製, 第2回ソフトマター研究会, Sep. 2012.
80.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : 剛体球系のモンテカルロシミュレーションから示唆されるコロイドエピタキシーにおける重力テンパリングの可能性, 日本物理学会2012年秋季大会, Sep. 2012.
81.	Atsushi Mori, Shingo Azuma, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, Tsutomu Sawada, Shuichi Hashimoto and Masanobu Haraguchi : フォトニックバンドとプラズモニクスのハイブリッド効果を起こすナノ構造の作製, 化学工学会第44回秋季大会, Sep. 2012.
82.	Yohei Yamada, 外山翔太, James METSON, David WILLIAMS, Geoff WATERHOUSE, Yoshihisa Suzuki, Tomoki Yabutani and Toshio Takayanagi : タンパク質結晶を鋳型とした規則性多孔質材料の作製, 日本分析化学会第61年会, 165, Sep. 2012.
83.	Shingo Azuma, Atsushi Mori, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, Tsutomu Sawada, Shuichi Hashimoto and Masanobu Haraguchi : フォトニックバンドとプラズモニクスのハイブリッドによる強力な電場増強効果を起こすナノ構造の作製, 第61回高分子討論会, Sep. 2012.
84.	Atsushi Mori, Shingo Azuma, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, Tsutomu Sawada, Shuichi Hashimoto and Masanobu Haraguchi : プラズモニクスのハイブリッド効果による強い電場増強効果を起こすナノ構造の作製を目指したゲル固定化コロイド結晶の作製, 日本物理学会2012年秋季大会, Sep. 2012.
85.	Kohei Hosokawa and Yoshihisa Suzuki : Crystallization and solubility measurements of a protein without using a precipitant, 日本物理学会第67回年次大会, Mar. 2012.
86.	Yoshihisa Suzuki and Hideaki Tsuge : Novel protein crystallization method without using a precipitant, 日本物理学会第67回年次大会, Mar. 2012.
87.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : 剛体球模型を用いたコロイドエピタキシーのモンテカルロ・シミュレーションにおける多結晶化の回避, 第25回分子シミュレーション討論会, Dec. 2011.
88.	近藤大貴, Takahisa Fujiwara and Yoshihisa Suzuki : リゾチーム正方晶系結晶のステップ前進速度の温度依存性, 2011年日本化学会西日本大会講演要旨集, 171, Nov. 2011.
89.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : ニワトリ卵白リゾチーム正方晶系結晶の{110}面上のステップ前進速度に及ぼす圧力効果, 第41回結晶成長国内会議, Nov. 2011.
90.	Yoshihisa Suzuki : 沈澱剤を用いないタンパク質の結晶化, 第41回結晶成長国内会議, Nov. 2011.
91.	Yoshiomi Matsumoto, Shin-ichiro Yanagiya and Yoshihisa Suzuki : グルコースイソメラーゼ結晶のステップ前進速度の温度依存性と活性化エネルギー, 第41回結晶成長国内会議, Nov. 2011.
92.	橋本華織, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : 逆三角形型セルを利用した遠心沈降法でのコロイド結晶の大型グレインの作製, 第41回結晶成長国内会議, Nov. 2011.
93.	坂部真貴子, Yoshihisa Suzuki, 藤原貴久, 上原侑紀, Atsushi Mori, Shin-ichiro Yanagiya, 佐藤正英 and Katsuhiro Tamura : コロイド結晶内のグレイン境界の時間変化, 第41回結晶成長国内会議, Nov. 2011.
94.	吉田尚貴, 東伸悟, 室井佑介, 坂部真貴子, 上原侑紀, Atsushi Mori and Yoshihisa Suzuki : 蛍光PMMAコロイド粒子の合成と共晶点顕微鏡による三次元像解析, 第41回結晶成長国内会議, Nov. 2011.
95.	Yoshihisa Suzuki : 遠心場でのコロイド結晶成長, 第41回結晶成長国内会議, Nov. 2011.
96.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : 正方格子パターン上へ成長した剛体球系結晶中の欠陥の振舞のモンテカルロシミュレーションによる研究, 第41回結晶成長国内会議, Nov. 2011.
97.	上原侑紀, Yoshihisa Suzuki, 坂部真貴子, Shin-ichiro Yanagiya and Atsushi Mori : 光ピンセット効果の粒子収集プロセスを用いたコロイド結晶の局所的核生成過程のその場観察, 第41回結晶成長国内会議, Nov. 2011.
98.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : 正方格子パターン上へ成長した剛体球系結晶中の欠陥の振舞のモンテカルロシミュレーションによる研究, 第60回高分子討論会, Sep. 2011.
99.	坂部真貴子, Yoshihisa Suzuki, Takahisa Fujiwara, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : コロイド結晶の格子欠陥の時間変化, 日本物理学会2011年秋季大会, Sep. 2011.
100.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : 高圧力下におけるニワトリ卵白リゾチーム正方晶系結晶のステップ前進速度, 日本物理学会2011年秋季大会, Sep. 2011.
101.	Yoshihisa Suzuki, Gen Sazaki, Takahisa Fujiwara and 古川義純 : ポリスチレン粒子水分散液の一方向凍結プロセスにおけるコロイド結晶化および不可逆凝集, 日本物理学会2011年秋季大会, Sep. 2011.
102.	Atsushi Mori, Yoshihisa Suzuki and Shigeki Matsuo : 正方格子パターン上へ成長した剛体球系結晶中の欠陥の振舞のモンテカルロシミュレーションによる研究, 第一回ソフトマター研究会, Aug. 2011.
103.	橋本華織, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : 逆三角型セルを利用したコロイド結晶の大型グレインの作成, 化学工学会第3回化学工学3支部合同徳島大会, Oct. 2010.
104.	坂部真貴子, Yoshihisa Suzuki, Takahisa Fujiwara, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : コロイド結晶内の粒子層の積層状態の経時変化, 化学工学会第3回化学工学3支部合同徳島大会, Oct. 2010.
105.	Yuuki Misumi, Yoshihisa Suzuki, Katsuhiro Tamura and Katsuhiro Tamura : ガス加圧法による枯草菌の殺菌, 化学工学3支部合同徳島大会講演要旨集, 136, Oct. 2010.
106.	Misa Hirano, Yoshihisa Suzuki and Katsuhiro Tamura : ニワトリ卵白リゾチーム正方晶系結晶のステップ前進速度に及ぼすpHの効果, 化学工学3支部合同徳島大会講演要旨集, 87, Oct. 2010.
107.	Yoshiomi Matsumoto, Yoshihisa Suzuki, Shin-ichiro Yanagiya and Katsuhiro Tamura : グルコースイソメラーゼ結晶のステップ前進速度の測定と活性化エネルギー, 化学工学3支部合同徳島大会講演要旨集, 86, Oct. 2010.
108.	Satoshi Kawachi, Yoshio Hara, Masafumi Ichimiya, Yoshihisa Suzuki and Katsuhiro Tamura : ガス加圧下の微生物代謝熱測定による気体毒性評価, 化学工学3支部合同徳島大会講演要旨集, 12, Oct. 2010.
109.	Norihiro Hosoi, Satoshi Kawachi, Yoshihisa Suzuki and Katsuhiro Tamura : 酸素・窒素ガスハイブリッド加圧による香酸柑橘果汁の殺菌, 化学工学3支部合同徳島大会講演要旨集, 1, Oct. 2010.
110.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : リゾチーム正方晶系結晶のステップ前進速度に及ぼす圧力効果, 化学工学3支部合同徳島大会講演要旨集, 89, Oct. 2010.
111.	Yoshihisa Suzuki, Atsushi Mori, Tsutomu Sawada and Katsuhiro Tamura : Crystallization control by centrifugation, 第57回応用物理学関係連合講演会, Mar. 2010.
112.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Fabrication of Bulk Close-Packed Colloidal Crystals by Centrifugation with Tilting a Crystallization Container, NCCG-39予稿集, 185, Nov. 2009.
113.	Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : Activation Volume of Glucose Isomerase for Elementary Processes of Crystallization, NCCG-39予稿集, 130, Nov. 2009.
114.	Takahisa Fujiwara, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Comparisons of the solubility measured by observation of the apices of tetragonal lysozyme crystals with that obtained by step velocity measurements, NCCG-39予稿集, 9, Nov. 2009.
115.	Makiko Sakabe, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Structure determination and defect observation of colloidal crystals by the use of a confocal fluorescent microscope, NCCG-39予稿集, 56, Nov. 2009.
116.	Kaori Hashimoto, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : Enlargement and nucleation control of the grains of the colloidal crystals by using an inverted triangle shaped container, NCCG-39予稿集, 55, Nov. 2009.
117.	Yoshihisa Suzuki : Enlargement of close-packed colloidal crystals by centrifugation, 2009年秋季大会領域9，領域12合同シンポジウム主題:コロイド・巨大分子の結晶成長, Sep. 2009.
118.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : 遠心沈降法による微粒子の自己集積過程, 2008日本化学会西日本大会, Nov. 2008.
119.	Naosuke Uchida, Yoshihisa Suzuki and Katsuhiro Tamura : 正方晶系リゾチーム結晶のステップ前進速度に及ぼすpHの効果, 2008日本化学会西日本大会, Nov. 2008.
120.	Tanaka Takanori, Yoshihisa Suzuki and Katsuhiro Tamura : Measurements of step velocities on the surface of monoclinic lysozyme crystals by laser confocal microscopy combined with differential interference contrast microscopy, NCCG-38予稿集, 31, Nov. 2008.
121.	Takahisa Fujiwara, Yoshihisa Suzuki and Katsuhiro Tamura : Solubility measurements by in situ observation of the vertices and steps of tetragonal lysozyme crystals, NCCG-38予稿集, 30, Nov. 2008.
122.	Makiko Sakabe, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Observation of self-assembly process during sedimentation of submicron particles in fluorescent solution with a confocal microscope, NCCG-38予稿集, 6, Nov. 2008.
123.	Kaori Hashimoto, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : A use of the container shape for grain-size control of colloidal crystals, NCCG-38予稿集, 5, Nov. 2008.
124.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Fabrication of close-packed bulk colloidal crystals by centrifugation, NCCG-38予稿集, 4, Nov. 2008.
125.	Atsushi Mori and Yoshihisa Suzuki : 正方格子パターン上への剛体球系結晶の成長, Meeting abstracts of the Physical Society of Japan, Vol.63, No.2, 332, Sep. 2008.
126.	Katsuaki Hama, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Growth and charactgerization of binary colloidal crystals obtained by settling large particles in the dispersion of small particles, 第61回コロイドおよび界面化学討論会講演概要集, 366, Sep. 2008.
127.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Fabrication of bulky close-packed colloidal crystals by centrifugation, 第61回コロイドおよび界面化学討論会講演概要集, 98, Sep. 2008. (Summary) Bulky close-packed colloidal crystals (>0.6x1.0x3.7mm^3) on a tilted cell wall were obtained by centrifugation.
128.	Atsushi Mori, 小林正裕 and Yoshihisa Suzuki : Stacking disorder in close-pack colloidal crystals of fcc (111) stacking, 第61回コロイドおよび界面化学討論会講演概要集, 97, Sep. 2008. (Summary) We have successfully demonstrated a dislocation mechanism of disappearance of a stacking fault in fcc (001) stacking by a Monte Carlo (MC) simulation of hard spheres under gravity [2]. In the case of fcc (111) stacking, however, the stacking faults cannot disappear completely; mixing state of random hexagonal close pack (rhcp) and fcc or faulted twinned fcc has been suggested. Figure 1 is a snapshot of a MC simulation viewed from a side of the stacking direction; fcc stackings of ABC and ACB types (straight lines toward upper-right and upper-left) are jointed by hexagonal close pack sequence (zigzag) between them. A trend of faulted twinned fcc has been seen in this figure.gelation.
129.	Masahiro Kobayashi, Atsushi Mori and Yoshihisa Suzuki : 重力下で{111}成長したの剛体球系結晶中の積層不整のモンテカルロシミュレーション, 第21回分子シミュレーション討論会講演概要集, Nov. 2007.
130.	Takahisa Fujiwara, Yoshihisa Suzuki and Katsuhiro Tamura : Solubility measurements by in situ observation of the vertices of tetragonal lysozyme crystals, NCCG-37予稿集, 97, Nov. 2007.
131.	Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba and Katsuhiro Tamura : Effects of high pressure on Glucose Isomerase structure observed by X-ray crystallography under 0.1 MPa, NCCG-37予稿集, 43, Nov. 2007.
132.	Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Grain Size Control on Flat Substrates by Centrifugation, NCCG-37予稿集, 42, Nov. 2007.
133.	Katsuaki Hama, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Growth and characterization of binary colloidal crystals obtained by centrifugation, 第60回コロイドおよび界面化学討論会講演概要集, 377, Sep. 2007.
134.	Harutoshi Asakawa, Yoshihisa Suzuki, Atsushi Mori and Katsuhiro Tamura : Effects of surface flatness of glass substrates on the orientation and size of the grains of colloidal crystals obtained by centrifugation, 第60回コロイドおよび界面化学討論会講演概要集, 376, Sep. 2007.
135.	Yoshihisa Suzuki, Atsushi Mori, Masashi Kasuga and Katsuhiro Tamura : Control of grain size of colloidal crystals on tilted glass substrates by centrifugation, 第60回コロイドおよび界面化学討論会講演概要集, 153, Sep. 2007.
136.	Atsushi Mori and Yoshihisa Suzuki : Stability of stacking disorder in hard-sphere crystal under gravity, Polymer Preprints, Japan, Vol.56, No.2, Sep. 2007. (Summary) On the reduction of stacking disorder in colloidal crystals due to gravity, demonstrations have been successful in part by Monte Carlo simulations. Along with the disappearance of defects, a sessile behavior has been observed. Elastic theoretical considerations are given on an intrinsic stacking fault and the minimal twin band structure. Whereas a glissile nature is confirmed for the former, the sessile behavior for the latter is supported. Vanishing logarithmic term in the energy, results from dislocation dipole configuration, must be an indication of the sessile nature. (Keyword) colloidal crystal / gravity / stacking fault / Shockley partial dislocation / twin band structure / elastic theory
137.	Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba and Katsuhiro Tamura : グルコースイソメラーゼの三次元分子構造に及ぼす圧力効果, 第15回生物関連高圧研究会20周年記念シンポジウム, Sep. 2007.
138.	Yoshihisa Suzuki, Gen Sazaki and Katsuhiro Tamura : 位相差顕微鏡によるグルコースイソメラーゼ結晶表面の分子ステップ観察, 第15回生物関連高圧研究会20周年記念シンポジウム, Sep. 2007.
139.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : In situ observation of molecular steps on the surface of protein crystals under high pressure, 第47回高圧討論会講演要旨集, 299, Nov. 2006.
140.	逢坂明彦, 西村展洋, Atsushi Mori, Yoshihisa Suzuki, Shin-ichiro Yanagiya and Tetsuo Inoue : Evolution of particle size and shape in polystyrene latex synthesis, Journal of the Japanese Association for Crystal Growth, Vol.33, No.4, 341, Nov. 2006.
141.	Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima and Katsuhiro Tamura : Effects of high pressure on the step velocity and two-dimensional nucleation rate of glucose isomerase crystals, Journal of the Japanese Association for Crystal Growth, Vol.33, No.4, 350, Nov. 2006.
142.	Atsushi Mori and Yoshihisa Suzuki : Effect of gravity on colloidal crystal, Journal of the Japanese Association for Crystal Growth, Vol.33, No.4, 252-253, Nov. 2006. (Summary) Reviews are given on the effect of gravity on enhancement of crystallization and improvement of crystallinity in colloidal crystallization. We performed centrifugation experiments of polystyrene mincoparticle dispersion and Monte Carlo simulations of hard spheres under gravity; the experiments were conducted in conditions that particles were close to each other in the colloidal crystals. Experimental and simulation results agree with each other in wide variety of phenomena.
143.	Nobuhiro Nishimura, Yoshihisa Suzuki and Katsuhiro Tamura : 重力場でのコロイド結晶化の体積分率の塩濃度依存性, 化学工学会徳島大会講演要旨集, 49, Oct. 2006.
144.	Masaya Omori, Yoshihisa Suzuki and Katsuhiro Tamura : コロイド結晶の欠陥消滅過程とそれに及ぼす外場の影響, 化学工学会徳島大会講演要旨集, 48, Oct. 2006.
145.	Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima and Katsuhiro Tamura : タンパク質結晶表面のステップ前進速度および二次元核生成頻度の圧力変化, 化学工学会徳島大会講演要旨集, 47, Oct. 2006.
146.	Yoshio Hara, Satoshi Kawachi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母増殖に及ぼす高圧希ガスの影響-代謝熱測定法によるアプローチ-, 化学工学会徳島大会講演要旨集, Oct. 2006.
147.	Satoshi Kawachi, 原好男, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 代謝熱測定法による炭化水素ガス加圧下の酵母増殖挙動解析, 化学工学会徳島大会講演要旨集, Oct. 2006.
148.	Toshiaki Arao, 村本桂久, Yoshihisa Suzuki and Katsuhiro Tamura : 酸素・窒素ガスハイブリッド加圧法によるスダチ果汁殺菌技術の開発, 化学工学会徳島大会講演要旨集, Oct. 2006.
149.	Atsushi Mori, 小林正裕, Yoshihisa Suzuki, Shigeki Matsuo and Shin-ichiro Yanagiya : Monte Carlo simulation of sediment in a pyramidal pit in the hard-sphere sysrem under gravity, Meeting abstracts of the Physical Society of Japan, Vol.61, No.2, 282, Sep. 2006.
150.	Harutoshi Asakawa, Yoshihisa Suzuki, Atsushi Mori, Shigeki Matsuo, Shin-ichiro Yanagiya, Tetsuo Inoue and Katsuhiro Tamura : Effects of surface flatness on the grain size of the colloidal crystal obtained by centrifugation, 第59回コロイドおよび界面化学討論会講演概要集, 350, Sep. 2006.
151.	Yoshihisa Suzuki, Atsushi Mori, Tsutomu Sawada and Katsuhiro Tamura : Control of grain boundaries and stacking disorders in colloidal crystals by centrifugation, 第59回コロイドおよび界面化学討論会講演概要集, 50, Sep. 2006.
152.	Atsushi Mori, 小林正裕 and Yoshihisa Suzuki : Sessile structure of complex of stacking disorders in hard-sphere crystal under gravity, 第59回コロイドおよび界面化学討論会講演概要集, 51, Sep. 2006. (Summary) We have preformed a Monte Carlo simulation of hard spheres under gravity. The strength of the gravity has been increased stepwise as similarly as a stepwise control of the rotation rate in a centrifugal experiment. We already reported the improvement of crystallinity, as well as the enhancement of crystallization, due to gravity; disappearance of a stacking fault was demonstrated. Here, we report on the sessile structures of staking disorders, which have remained under gravity. Those structures are in contrast to the single stacking faults that disappear via glide of the Shockley partial dislocation. Figure 1 is one of sessile structure; we see twin bands made of two layers. The sides of the bands are terminated by three Shockley partial dislocations. We have found that some stacking disorders easily disappear under gravity and some complex structures do not. An account is given for experimental fact that the stacking disorders do not disappear completely under gravity.
153.	小林正裕, Atsushi Mori, Shin-ichiro Yanagiya, Yoshihisa Suzuki and Tetsuo Inoue : Monte Carlo simulation of defect in hard-sphere crystal under gravity, 応用物理学会中四国支部日本物理学会中国支部・四国支部日本物理教育学会四国連絡会議支部学術講演会講演予稿集, 150, Jul. 2006.
154.	藤本直樹, 佐藤秀樹, Toshiaki Arao, Yoshihisa Suzuki, Katsuhiro Tamura and 高田次郎 : Bioassay of Various Pesticides by Measuring Metabolic Heat of Microorganisms, 第40回日本水環境学会年会, Mar. 2006.
155.	Katsuhiro Tamura, Toshiaki Arao and Yoshihisa Suzuki : Bioassay of Several Gases by Measuring Metabolic Heat of Microorganisms, 第40回日本水環境学会年会, Mar. 2006.
156.	春日聖司, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya, Tetsuo Inoue and Katsuhiro Tamura : 濃厚なコロイド結晶のグレインサイズの塩濃度,遠心加速度,粘性率,角度依存性, 日本物理学会第61回年次大会, Vol.61, No.1, 384, Mar. 2006.
157.	麻川明俊, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya, Tetsuo Inoue and Katsuhiro Tamura : 遠心沈降濃縮法で得られたコロイド結晶のグレインサイズの基板依存性, 日本物理学会第61回年次大会, Vol.61, No.1, 384, Mar. 2006.
158.	Yoshihisa Suzuki, Tsutomu Sawada, Atsushi Mori and Katsuhiro Tamura : 遠心沈降濃縮法によるコロイド結晶のグレインサイズ制御, 日本物理学会第61回年次大会, Vol.61, No.1, 383, Mar. 2006.
159.	Atsushi Mori, Yoshihisa Suzuki and Shin-ichiro Yanagiya : 重力下の剛体球系における多様な構造に対する自由エネルギー計算, Meeting abstracts of the Physical Society of Japan, Vol.61, No.1, 384, Mar. 2006.
160.	Atsushi Mori, 湯浅元明, Shin-ichiro Yanagiya and Yoshihisa Suzuki : 重力下の剛体球系結晶のモンテカルロシミュレーション, 第19回分子シミュレーション討論会講演概要集, Nov. 2005.
161.	岩橋均, 小谷峰, 松岡寛之, 北河恵美子, 石藤恵美, Yoshihisa Suzuki and Katsuhiro Tamura : 各種高圧条件が酵母細胞に与える影響, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 22, Sep. 2005.
162.	松岡寛之, 鈴木福英, 岩橋均, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母DNAマイクロアレイを用いた気体のバイオアッセイ, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 21, Sep. 2005.
163.	栗林努, 松岡寛之, 鈴木福英, 岩橋均, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母DNAマイクロアレイを用いた二酸化炭素の影響評価, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 20, Sep. 2005.
164.	中山真一, 原好男, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母の代謝熱測定による加圧気体の毒性評価, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 19, Sep. 2005.
165.	谷脇孝典, 村本桂久, Yoshihisa Suzuki and Katsuhiro Tamura : ガス加圧法によるスダチ果汁の殺菌と酸化防止技術, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 18, Sep. 2005.
166.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : タンパク質結晶表面のステップ前進速度に及ぼす圧力効果, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 10, Sep. 2005.
167.	近田恵美, Yoshihisa Suzuki, 本同宏成 and Katsuhiro Tamura : リゾチーム三斜晶系結晶の溶解度に及ぼす圧力温度pH効果, 第14回生物関連高圧研究会シンポジウム「高圧力下の生物科学」講演要旨集, 9, Sep. 2005.
168.	Atsushi Mori, 湯浅元明, Shin-ichiro Yanagiya, Yoshihisa Suzuki and Tetsuo Inoue : Monte Carlo simulation study on the effect of boundary condition on the crystallization of hard spheres under gravity, Meeting abstracts of the Physical Society of Japan, Vol.60, No.2, 232, Sep. 2005.
169.	大森政也, Yoshihisa Suzuki, Shin-ichiro Yanagiya, Atsushi Mori, Tetsuo Inoue and Katsuhiro Tamura : コロイド結晶のグレインサイズに及ぼす磁場効果, 第58回コロイドおよび界面化学討論会講演要旨集, 280, Sep. 2005.
170.	Atsushi Mori, Yuuma Kitadai, Motoaki Yuasa, Shin-ichiro Yanagiya, Yoshihisa Suzuki, Tsutomu Sawada and Tetsuo Inoue : Monte Carlo simulation of competing crystals grown from walls in the hard sphere model under gravity, 第58回コロイドおよび界面化学討論会講演概要集, 131, Sep. 2005.
171.	Masashi Kasuga, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya, Tetsuo Inoue and Katsuhiro Tamura : 濃厚なコロイド結晶のドメインサイズの塩濃度，遠心加速度，粘性率依存性, 第58回コロイドおよび界面化学討論会講演概要集, 279, Sep. 2005.
172.	Yoshihisa Suzuki, Tsutomu Sawada, Younan Xia, Atsushi Mori and Katsuhiro Tamura : Domain size control of colloidal crystals by the control of sedimentation rates, 第58回コロイドおよび界面化学討論会講演要旨集, 130, Sep. 2005.
173.	村田壮介, Shin-ichiro Yanagiya, Atsushi Mori, Yoshihisa Suzuki, Katsuhiro Tamura and Tetsuo Inoue : TGS水溶液のオーバーヒーティングが結晶成長に及ぼす影響, Journal of the Japanese Association for Crystal Growth, Vol.32, No.3, 223, Aug. 2005.
174.	近田恵美, Yoshihisa Suzuki, 本同宏成 and Katsuhiro Tamura : リゾチーム三斜晶系結晶の溶解度に及ぼす圧力温度効果, 第35回日本結晶成長学会誌, 230, Aug. 2005.
175.	Yoshihisa Suzuki, Gen Sazaki, 松本雅光, 永澤眞, 中嶋一雄 and Katsuhiro Tamura : 高圧力下におけるグルコースイソメラーゼ結晶表面上の単位ステップのその場観察, 第35回日本結晶成長学会誌, 228, Aug. 2005.
176.	下村英之, Yoshihisa Suzuki, 丸岡武史 and Katsuhiro Tamura : グルコースイソメラーゼ結晶の核生成頻度に及ぼす圧力効果, 第35回日本結晶成長学会誌, 183, Aug. 2005.
177.	大森政也, Yoshihisa Suzuki, Shin-ichiro Yanagiya, Atsushi Mori, Tetsuo Inoue and Katsuhiro Tamura : コロイド結晶のグレインサイズに及ぼす磁場効果, 第35回日本結晶成長学会誌, 182, Aug. 2005.
178.	春日聖司, Yoshihisa Suzuki, Atsushi Mori, Shin-ichiro Yanagiya, Tetsuo Inoue and Katsuhiro Tamura : 濃厚なコロイド結晶のグレインサイズ制御, 第35回日本結晶成長学会誌, 181, Aug. 2005.
179.	Yoshihisa Suzuki, Tsutomu Sawada, Younan Xia, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : 沈降法によるコロイド結晶化制御, 第35回日本結晶成長学会誌, 180, Aug. 2005.
180.	Atsushi Mori, 湯浅元明, Shin-ichiro Yanagiya, Yoshihisa Suzuki and Tetsuo Inoue : Enlargement of hard-sphere crystal by sudden increase of gravitational number, Journal of the Japanese Association for Crystal Growth, Vol.32, No.3, 184, Aug. 2005.
181.	北台佑馬, Atsushi Mori, Shin-ichiro Yanagiya, Yoshihisa Suzuki and Tetsuo Inoue : 重力下における剛体球系結晶の欠陥消失機構, 第18回分子シミュレーション討論会講演要旨集, 123-124, Dec. 2004.
182.	Yoshihisa Suzuki, Gen Sazaki, Yoshiki Matsuura, Hironori Hondoh, Haruhiko Sakuraba, Toshihisa Ohshima, Kazuo Nakajima and Katsuhiro Tamura : 高圧力下におけるグルコースイソメラーゼ結晶の成長促進機構に関する分子論的考察, 第45回高圧討論会, Oct. 2004.
183.	Yoshihisa Suzuki, Younan Xia, Tsutomu Sawada, Atsushi Mori and Katsuhiro Tamura : Colloidal crystallization by sedimentation, 第57回コロイドおよび界面化学討論会講演要旨集, 373, Sep. 2004.
184.	Yoshihisa Suzuki, Gen Sazaki, Yoshiki Matsuura, Hironori Hondoh, Haruhiko Sakuraba, Toshihisa Ohshima, Kazuo Nakajima and Katsuhiro Tamura : A molecular-level discussion on the growth kinetics of glucose isomerase crystals, 第34回結晶成長国内会議, Aug. 2004.
185.	Masashi Kasuga, Yoshihisa Suzuki, Atsushi Mori, Tetsuo Inoue and Katsuhiro Tamura : Growth of a photonic particulate crystal using a sedimentation method, 第34回結晶成長国内会議, Vol.31, No.3, 163, Aug. 2004.
186.	Hiroyuki Matsuoka, 鈴木福英, Hitoshi Iwahashi, Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母DNAマイクロアレイを用いた加圧窒素ガスのストレス応答解析, 生物関連高圧研究会第13回シンポジウム, Sep. 2003.
187.	Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 酵母のガス加圧増殖サーモグラムとその利用, 生物関連高圧研究会第13回シンポジウム, Sep. 2003.
188.	Toshitaka Asai, Yoshihisa Suzuki and Katsuhiro Tamura : ニワトリ卵白リゾチーム単斜晶系結晶の成長機構に及ぼす圧力，温度効果, 生物関連高圧研究会第13回シンポジウム, Sep. 2003.
189.	Takeshi Maruoka, Yoshihisa Suzuki and Katsuhiro Tamura : グルコースイソメラーゼの結晶成長に及ぼす圧力，温度，塩濃度効果, 生物関連高圧研究会第13回シンポジウム, Sep. 2003.
190.	三好康夫, 中原将海, Atsushi Mori, Yoshihisa Suzuki, Tsutomu Sawada, Kensaku Ito, Shin-ichiro Yanagiya and Tetsuo Inoue : 重力下の剛体球結晶の構造:モンテカルロシミュレーション, Journal of the Japanese Association for Crystal Growth, Vol.30, No.3, 129, Aug. 2003.
191.	中原将海, 三好康夫, Shin-ichiro Yanagiya, Atsushi Mori, Yoshihisa Suzuki, Tsutomu Sawada, Kensaku Ito and Tetsuo Inoue : 遮蔽クーロン系結晶の分子動力学シミュレーション, Journal of the Japanese Association for Crystal Growth, Vol.30, No.3, 128, Aug. 2003.
192.	Yoshihisa Suzuki, 佐崎元, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : 高圧力下におけるグルコースイソメラーゼの結晶化制御, 日本物理学会年会, Vol.58, Mar. 2003. (Summary) グルコースイソメラーゼは，平衡論的にも速度論的にも加圧により結晶化が大きく促進されるタンパク質である．このようなタンパク質の結晶化機構の解明は，未結晶化·難結晶化タンパク質の結晶化に役立つであろう．今回は，より詳細な速度論的な解析をすすめる一方，実際に高圧力下で結晶化をコントロールした結果について報告する．
193.	三好康夫, 中原将海, Atsushi Mori, Yoshihisa Suzuki, Shin-ichiro Yanagiya, Tetsuo Inoue, Tsutomu Sawada and Kensaku Ito : 重力下の剛体球系の固液共存状態のモンテカルロシミュレーション, Meeting abstracts of the Physical Society of Japan, Vol.58, No.1, 336, Mar. 2003.
194.	Yoshihisa Suzuki, 佐崎元, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : 加圧によるタンパク質結晶化の促進, 第43回高圧討論会, Nov. 2002.
195.	施寧, Yoshihisa Suzuki, Katsuhiro Tamura and Tsutomu Sawada : コロイド結晶化·凝集条件の塩濃度依存性, 日本物理学会2002年秋季大会, Sep. 2002.
196.	三好康夫, 斎藤好延, Atsushi Mori, Yoshihisa Suzuki, Tsutomu Sawada and Kensaku Ito : 重力下の剛体球系の結晶化のモンテカルロシミュレーション, Journal of the Japanese Association for Crystal Growth, Vol.29, No.2, 87, Aug. 2002.
197.	Yoshihisa Suzuki, 佐崎元, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : グルコースイソメラーゼの結晶成長に及ぼす圧力効果の速度論的研究, 第32回結晶成長国内会議, Aug. 2002.
198.	Yoshihisa Suzuki, 佐崎元, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : 加圧によるグルコースイソメラーゼ結晶成長の促進効果, 日本物理学会第57回年次大会, Mar. 2002.
199.	Katsuhiro Tamura, Toshiaki Arao, 朝倉光昭, Yoshihisa Suzuki, 高島京子 and 土佐政二 : 微生物の代謝熱を利用した気圏および水圏環境汚染物質のバイオアッセイ, 第36回日本水環境学会年会, Mar. 2002.
200.	Tsutomu Sawada, 豊玉彰子 and Yoshihisa Suzuki : フォトニック結晶としてのコロイド単結晶の作製と分光特性, 第40回人工結晶討論会, Dec. 2001.
201.	Toshiaki Arao, Yoshihisa Suzuki and Katsuhiro Tamura : 代謝熱測定による酵母のストレス応答の研究 -糖の影響-, 日本生物物理学会第39回年会, Oct. 2001.
202.	松本知浩, Katsuhiro Tamura and Yoshihisa Suzuki : リン脂質二分子膜のゲル-液晶主転移温度に及ぼす圧力の影響, 第39回日本生物物理学会, Oct. 2001.
203.	Tsutomu Sawada, Yoshihisa Suzuki and 豊玉彰子 : せん断流動のクエンチによるコロイド結晶の単結晶化, 第54回コロイドおよび界面化学討論会, Sep. 2001.
204.	Katsuhiro Tamura, 松本知浩, Toshiaki Arao and Yoshihisa Suzuki : ガス圧高圧熱量計の応用, 第12回生物関連高圧研究会シンポジウム, Sep. 2001.
205.	Yoshihisa Suzuki, 佐崎元, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : 加圧によるタンパク質結晶の成長促進効果, 第12回生物関連高圧研究会シンポジウム, Sep. 2001.
206.	Katsuhiro Tamura, 松本知浩, Toshiaki Arao and Yoshihisa Suzuki : リン脂質二分子膜の相転移に及ぼす溶存気体の影響, 第19回麻酔メカニズム研究会, Jul. 2001.
207.	Yoshihisa Suzuki and Tsutomu Sawada : 濃厚な単分散粒子溶液における凝集挙動, 第31回結晶成長国内会議, Jul. 2000.
208.	Yoshihisa Suzuki and Tsutomu Sawada : コロイド粒子の凝集挙動の塩濃度依存性, 第30回結晶成長国内会議, Jul. 1999.
209.	Yoshihisa Suzuki, 澤村精治, 松尾博史, 高木友裕 and 藤本修 : アミノ酸の水に対する溶解度の圧力依存性, 第29回結晶成長国内会議, Jul. 1998.
210.	Yoshihisa Suzuki, Tsutomu Sawada, 宮下哲 and 小松啓 : HEW lysozyme正方晶の成長kineticsへの圧力効果II, 第28回結晶成長国内会議, Jul. 1997.
211.	Yoshihisa Suzuki, Tsutomu Sawada, 宮下哲 and 小松啓 : HEW lysozyme正方晶の成長kineticsへの圧力効果, 第27回結晶成長国内会議, Aug. 1996.
212.	Yoshihisa Suzuki, Tsutomu Sawada, 宮下哲 and 小松啓 : 圧力制御によるタンパク質の結晶成長, 第51回日本物理学会年会「結晶成長シンポジウム」, Apr. 1996.
213.	Yoshihisa Suzuki, Tsutomu Sawada, 宮下哲 and 小松啓 : ニワトリ卵白リゾチームの溶解度の圧力依存の''その場観察'', 日本物理学会秋の分科会, Sep. 1995.
214.	Yoshihisa Suzuki, 宮下哲, 小松啓, 八木健彦 and 佐藤清隆 : 高圧力下でのHEWリゾチームの結晶成長II, 第25回結晶成長国内会議, Jul. 1994.
215.	Yoshihisa Suzuki, 宮下哲, 小松啓, 八木健彦 and 佐藤清隆 : 高圧力下でのHEWリゾチームの結晶成長, 第24回結晶成長国内会議, Jul. 1993.

Et cetera, Workshop:

1.	Yutaka Tsuboi, Masahiro Kanno, Shigeki Matsuo and Yoshihisa Suzuki : 引力系コロイド結晶の結晶成長素過程, 結晶表面・界面での成長カイネティクスの理論とその場観察, Jan. 2023.
2.	Yoshihisa Suzuki, Keigo Kishida, Hiroyasu Katsuno and Masahide Sato : 板状引力系コロイド結晶の臨界核近傍のクラスター形成・分解プロセス, 結晶表面・界面での相転移ダイナミクスのその場観察と理論の新展開, Jan. 2023.
3.	Yoshihisa Suzuki, Ai Ninomiya, Seijiro Fukuyama, Taro Shimaoka, Masae Nagai, Koji Inaka, Takehiko Sone, Shingo Wachi, Katsuo Tsukamoto and Yasutomo Arai : 微小重力下におけるグルコースイソメラーゼ結晶の沿面成長速度, 結晶表面・界面での成長カイネティクスの理論とその場観察, Jan. 2020.
4.	Yoshihisa Suzuki : 沈澱剤を用いないタンパク質結晶化および結晶構造解析, 結晶表面・界面での成長カイネティクスの理論とその場観察, Jan. 2016.
5.	Yoshihisa Suzuki : 環境相の状態変化に伴うタンパク質結晶表面カイネティックスの変化, 学習院大学計算機センター特別研究プロジェクト「結晶成長の数理」第9回研究会―濡れと結晶成長―, Dec. 2014.
6.	Yoshihisa Suzuki, Takahisa Fujiwara, 細川晃平 and 塩澤宏章 : タンパク質結晶化への新たなる試み, 結晶表面・界面での成長カイネティクスの理論とその場観察, Jan. 2014.
7.	Atsushi Mori and Yoshihisa Suzuki : コロイドエピタキシーにおける重力テンパリングによるコロイド結晶中の欠陥の低減, 学習院大学計算機センター特別企画「結晶成長の数理」第8回研究会―濡れと結晶成長―, Dec. 2013.
8.	Yoshihisa Suzuki : 相互作用の制御によるナノ粒子の結晶化制御, その場観察と理論による結晶成長素過程の解明, Jan. 2013.
9.	Yoshihisa Suzuki : 相互作用の制御によるナノ粒子の規則構造形成, 学習院大学計算機センター特別研究プロジェクト「結晶成長の数理」第7回研究会―ソフトマターと結晶成長―, Dec. 2012.
10.	Atsushi Mori and Yoshihisa Suzuki : 化学ポテンシャル差で表現した核生成可逆仕事におけるバルク項の表式, 学習院大学計算機センター特別企画「結晶成長の数理」第7回研究会―ソフトマターと結晶成長―, Dec. 2012.
11.	Shingo Azuma, Atsushi Mori, Keisuke Ohkubo, Yoshihisa Suzuki, 豊玉彰子, 山中淳平, 澤田勉, Shuichi Hashimoto and Masanobu Haraguchi : フォトニックバンドとプラズモニクスのハイブリッドによる強力な電場増強効果を起こすナノ構造の作製, 学習院大学計算機センター特別研究プロジェクト「結晶成長の数理」第7回研究会―ソフトマターと結晶成長―, Dec. 2012.
12.	Yoshihisa Suzuki : ナノ∼サブミクロン粒子の結晶成長, 第36回結晶成長討論会, Sep. 2012.
13.	Yoshihisa Suzuki : ナノ∼サブミクロン粒子を単位とした結晶化およびその結晶の高品質化, 化学工学会材料界面部会晶析技術研究会夏季セミナー2012in徳島, Sep. 2012.
14.	Yoshihisa Suzuki and Atsushi Mori : 核生成その1, 第35回結晶成長討論会結晶成長基礎講習会シリーズ1「結晶成長の基礎」, 1-6, Sep. 2011.
15.	Atsushi Mori and Yoshihisa Suzuki : 核生成その2, 第35回結晶成長討論会結晶成長基礎講習会シリーズ1「結晶成長の基礎」, 7-13, Sep. 2011.
16.	坂部真貴子, Yoshihisa Suzuki, Takahisa Fujiwara, Atsushi Mori, Shin-ichiro Yanagiya and Katsuhiro Tamura : 経時変化によるコロイド結晶内の格子欠陥の観察, 「結晶成長の数理」第五回研究会―鏡像対称性と結晶成長, Dec. 2010.
17.	Kaori Hashimoto, Yoshihisa Suzuki, Atsushi Mori, Katsuhiro Tamura and Katsuhiro Tamura : 結晶化容器形状を用いたコロイド結晶のグレインの肥大化, 「結晶成長の数理」第五回研究会―鏡像対称性と結晶成長, Dec. 2010.
18.	Yoshihisa Suzuki : 超濃厚タンパク溶液のX線回折, 「結晶成長の数理」第五回研究会―鏡像対称性と結晶成長, 9, Dec. 2010.
19.	Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Shin-ichiro Yanagiya and Katsuhiro Tamura : 高圧力下におけるリゾチーム正方晶系結晶のステップ前進速度, 「結晶成長の数理」第五回研究会―鏡像対称性と結晶成長, Dec. 2010.
20.	Atsushi Mori, Yoshihisa Suzuki, 金繁美希 and Shigeki Matsuo : 剛体球模型を用いたコロイドエピタキシーのシミュレーション, 学習院大学計算機センター特別企画「結晶成長の数理」第5回研究会, Dec. 2010.
21.	Atsushi Mori and Yoshihisa Suzuki : 重力下の剛体球系結晶におけるショックレーの部分転位の弾性場と重力による弾性場の相互作用, 第20回統計物理学研究会研究報告集, 55-75, May 2010.
22.	Yoshihisa Suzuki : 結晶成長学的視点で行ったコロイド結晶のグレインサイズ大型化, 平成21年度学習院大学計算機センター研究会講演資料集, 58-61, Dec. 2009.
23.	Takahisa Fujiwara, Yoshihisa Suzuki and Katsuhiro Tamura : リゾチーム正方晶系結晶のその場観察による溶解度測定法, 平成21年度学習院大学計算機センター研究会講演資料集, 19-22, Dec. 2009.
24.	Yoshihisa Suzuki : 沈降プロセスにおけるコロイド結晶の構造制御, 学習院大学計算機センター特別研究プロジェクト「結晶成長の数理」第三回研究会―パターン形成と構造の制御―, 376, Dec. 2008.
25.	浅井俊高, Yoshihisa Suzuki and Katsuhiro Tamura : ニワトリ卵白リゾチーム単斜晶系結晶の成長機構に及ぼす圧力，温度効果, 有機·生体物質の結晶化シンポジウム, Mar. 2003.
26.	丸岡武史, Yoshihisa Suzuki and Katsuhiro Tamura : グルコースイソメラーゼの結晶成長に及ぼす圧力，温度，塩濃度の効果, 有機·生体物質の結晶化シンポジウム, Mar. 2003.
27.	Yoshihisa Suzuki, 佐崎元, 松井拓郎, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : 高圧力下におけるグルコースイソメラーゼ結晶の速度論的解析, 有機·生体物質の結晶化シンポジウム, Mar. 2003.
28.	Yoshihisa Suzuki, 佐崎元, 松井拓郎, Kalevi Visuri, Katsuhiro Tamura and 中嶋一雄 : グルコースイソメラーゼの結晶成長kineticsに及ぼす圧力効果, 生体高分子結晶成長ワークショップ, Jan. 2003.
29.	Yoshihisa Suzuki : グルコースイソメラーゼ結晶の溶解度の圧力変化, 「タンパク質·核酸の結晶成長」ワークショップ, Feb. 2002.
30.	Yoshihisa Suzuki : たんぱく質の結晶化における圧力の可能性, 日本材料学会第149回高圧力部門委員会, Apr. 2001.
31.	Yoshihisa Suzuki, Tsutomu Sawada and Katsuhiro Tamura : 高粒子濃度におけるコロイド結晶化及び凝集条件の探索, 徳島大学サテライトベンチャービジネスラボラトリー平成12年度第3回講演会, Mar. 2001.
32.	Yoshihisa Suzuki, Satoru Miyashita, Tsutomu Sawada, Gen Sazaki, Toshitaka Nakada, Toshiaki Arao, Katsuhiro Tamura and Hiroshi Komatsu : Protein crystallization and high pressure, workshop Protein Crystallization and Interfacial Phenomena -Present and Future-, Feb. 2001.
33.	Yoshihisa Suzuki : lysozymeの結晶成長速度に及ぼす圧力効果, 「タンパク質·核酸の結晶成長」ワークショップ, Mar. 2000.
34.	Yoshihisa Suzuki : 高圧力下のアミノ酸の溶解度測定, 結晶成長学会バイオクリスタル分科会第6回研究会, Nov. 1998.
35.	Yoshihisa Suzuki : hen egg white lysozyme 正方晶系結晶の成長kineticsに及ぼす圧力効果, 第1回タンパク質結晶成長機構ワークショップ, Mar. 1998.
36.	Satoru Miyashita, Hiroshi Komatsu, Yoshihisa Suzuki and Toshitaka Nakada : Observation of the Concentration Distribution Around a Growing Lysozyme Crysta, Proc. 7th Topical Meeting on Crystal Growth Mechanism, 401-412, Jan. 1994.

Report:

1.	Tomoki Yabutani and Yoshihisa Suzuki : 化学応用工学科のFD活動, 平成24年度FD研究報告書, 24-34, Mar. 2013.
2.	Tomoki Yabutani, Yoshihisa Suzuki, Masaki Nishiuchi, Masashi Kurashina and Toshihide Horikawa : 化学応用工学科のFD活動について, 平成23年度FD研究報告書, 32-49, Feb. 2012.
3.	Shigeki Matsuo, Atsushi Mori and Yoshihisa Suzuki : Development of techniques for creating high-quality colloidal c, Bulletin of Faculty of Engineering, The University of Tokushima, Vol.50, Tokushima, 2005. (Tokushima University Institutional Repository) ● Metadata: 74504 (Tokushima University Institutional Repository: 74504)

Patent:

1.	Tsutomu Sawada and Yoshihisa Suzuki : コロイド結晶，並びにゲル化コロイド結晶の形成方法，及びこれに基づくコロイド結晶素子，ゲル化コロイド結晶素子，並びにコロイド結晶シートの作製方法と，作製されるコロイド結晶素子，ゲル化コロイド結晶素子，並びにコロイド結晶シート, 2000-217660 (Jul. 2000), 3533442 (Mar. 2004).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

狭い空間に束縛されたコロイド粒子によるコロイド合金形成-効率化と新奇構造の探索- (Project/Area Number: 23K03258 )
Presumption of statistical mechanical state distribution of 3D protein structure in solution states by crystallization (Project/Area Number: 21K04908 )
Study of unique structures and their formation processes formed by patchy particles-effects of anisotropies of interaction and particles shape (Project/Area Number: 20K03782 )
Crystallization and crystallography of proteins at in vivo salt concentrations (Project/Area Number: 18K04960 )
Effects of impurities on crystal growth-what changes when impurites move- (Project/Area Number: 16K05470 )
Utilization of membrane filter for a protein crystallization field and single- crystal X-ray diffraction analysis (Project/Area Number: 15K05668 )
Melt-like growth of protein crystals (Project/Area Number: 26390054 )
Colloidal crystallization of protein molecules (Project/Area Number: 24656016 )
Static and kinetic effect of impurities on instability of shape of crystal (Project/Area Number: 23540444 )
Recovery of rare metals by use of non-strong acid and non-nitrogen containing solvents from wastes (Project/Area Number: 22656207 )
Studies on the activation volume of an incorporation process by in situ measurements of step velocities under high pressure (Project/Area Number: 19760009 )
圧力変化によるタンパク質の立体構造変化と酵素活性の圧力依存性の相関 (Project/Area Number: 16760014 )
磁場配向させたゲルを利用した種々の結晶材料のゲル成長 (Project/Area Number: 16656012 )
Toxicity assessment of environmental pollution gas using yeast DNA microarray (Project/Area Number: 16510024 )
Bioassay of pollution gas by metabolic heat measurement of yeast (Project/Area Number: 13680629 )
Search by Researcher Number (60325248)

Professor : Suzuki, Yoshihisa

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)