Tokushima University / Educator and Researcher Directory --- Watanabe, Takahito

His Web Page

https://researchmap.jp/TakahitoWatanabe (researchmap)

Field of Study

○	Life sciences [Zoological sciences]
○	Life sciences [Developmental biology]
○	Life sciences [Molecular biology]

Subject of Study

Book / Paper

Book:

1.	Takahito Watanabe, Sumihare Noji and Taro Mito : GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, the Two-Spotted Cricket Gryllus bimaculatus, using TALENs. In TALENs: Methods and Protocols (Ralf Kuhn et al. eds.), Springer, New York, 2016. (Link to Publication Site) ● Publication site (DOI): 10.1007/978-1-4939-2932-0_12 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1007/978-1-4939-2932-0_12 (DOI: 10.1007/978-1-4939-2932-0_12)
2.	Takahito Watanabe, 松岡佑児, Sumihare Noji and Taro Mito : 進化するゲノム編集技術(真下知士, 城石俊彦監修)第2編第2章第4節フタホシコオロギにおけるゲノム編集, エヌ・ティー・エス, Jan. 2015.
3.	Takahito Watanabe, Taro Mito, Hideyo Ohuchi and Sumihare Noji : 第10章コオロギにおけるZFN，TALEN，CRISPR/Cas9を用いた遺伝子改変, 羊土社, Apr. 2014.

Academic Paper (Judged Full Paper):

1.	Aya Nakai, Akihito Tanaka, Hitoshi Yoshihara, Koji Murai, Takahito Watanabe and Katsuyuki Miyawaki : Blue LED light promotes indican accumulation and flowering in indigo plant, Polygonum tinctorium, Industrial Crops and Products, Vol.155, 112774, 2020. (Keyword) Polygonum tinctorium / Plant factory / Blue LED / Photoperiod / Flowering / Indican (Tokushima University Institutional Repository) ● Metadata: 114990 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.indcrop.2020.112774 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85088149064 (Tokushima University Institutional Repository: 114990, DOI: 10.1016/j.indcrop.2020.112774, Elsevier: Scopus)
2.	Yoshiyasu Ishimaru, Sayuri Tomonari, Takahito Watanabe, Sumihare Noji and Taro Mito : Regulatory mechanisms underlying the specification of the pupal-homologous stage in a hemimetabolous insect, Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, Vol.374, No.1783, 20190225, 2019. (Summary) Juvenile hormones and the genetic interaction between the transcription factors Krüppel homologue 1 (Kr-h1) and Broad (Br) regulate the transformation of insects from immature to adult forms in both types of metamorphosis (holometaboly with a pupal stage versus hemimetaboly with no pupal stage); however, knowledge about the exact instar in which this occurs is limited. Using the hemimetabolous cricket Gryllus bimaculatus (Gb), we demonstrate that a genetic interaction occurs among Gb'Kr-h1, Gb'Br and the adult-specifier transcription factor Gb'E93 from the sixth to final (eighth) nymphal instar. Gb'Kr-h1 and Gb'Br mRNAs were strongly expressed in the abdominal tissues of sixth instar nymphs, with precocious adult moults being induced by Gb'Kr-h1 or Gb'Br knockdown in the sixth instar. The depletion of Gb'Kr-h1 or Gb'Br upregulates Gb'E93 in the sixth instar. By contrast, Gb'E93 knockdown at the sixth instar prevents nymphs transitioning to adults, instead producing supernumerary nymphs. Gb'E93 also represses Gb'Kr-h1 and Gb'Br expression in the penultimate nymphal instar, demonstrating its important role in adult differentiation. Our results suggest that the regulatory mechanisms underlying the pupal transition in holometabolous insects are evolutionarily conserved in hemimetabolous G. bimaculatus, with the penultimate and final nymphal periods being equivalent to the pupal stage. This article is part of the theme issue 'The evolution of complete metamorphosis'. (Tokushima University Institutional Repository) ● Metadata: 113697 (Link to Publication Site) ● Publication site (DOI): 10.1098/rstb.2019.0225 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31438810 ● Search Scopus @ Elsevier (PMID): 31438810 ● Search Scopus @ Elsevier (DOI): 10.1098/rstb.2019.0225 (Tokushima University Institutional Repository: 113697, DOI: 10.1098/rstb.2019.0225, PubMed: 31438810)
3.	Kamenura Norio, Mayumi Sugimoto, Tamehiro Norimasa, Adachi Reiko, Sayuri Tomonari, Takahito Watanabe and Taro Mito : Cross-allergenicity of crustacean and the edible insect Gryllus bimaculatus in patients with shrimp allergy, Molecular Immunology, Vol.106, 127-134, 2019. (Summary) Food scarcity is a serious problem for many developing as well as developed countries. Edible insects have attracted attention recently as a novel food source. Crickets are especially high in nutritional value and easy to breed and harvest. In this study, we evaluated the risk of allergic reactions associated with cricket consumption in individuals with crustacean allergy. We evaluated food allergy risk in the consumption of Gryllus bimaculatus (cricket) in patients with shrimp allergy, using enzyme-linked immunosorbent assay (ELISA) and IgE crosslinking-induced luciferase expression assay (EXiLE). Sera from individuals with shrimp allergy (positive for shrimp-specific IgE by ImmunoCAP (>0.35 UA/mL; n = 9) or without shrimp allergy (negative for shrimp-specific IgE; n = 6) were obtained. There was a strong correlation between shrimp- and Gryllus-specific IgE levels obtained by ELISA (r = 0.99; P < 0.001). The binding of shrimp-specific IgE on shrimp allergen was dose-dependently inhibited by Gryllus allergen (0-1.0 mg/mL). There was a strong correlation between shrimp- and Gryllus-specific IgE responses, as assessed by EXiLE assays (r = 0.89; P < 0.001). We determined that a protein of approximately 40 kDa reacted with the positive, but not negative, sera for shrimp-specific IgE by ImmunoCAP. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis identified the major allergen in shrimp and Gryllus to be tropomyosin. Our data suggest that the cricket allergen has the potential to induce an allergic reaction in individuals with crustacean allergy. Therefore, allergy risk and shrimp-specific IgE levels should be considered before consumption of cricket meal. (Keyword) Adolescent / Adult / Allergens / Animals / Child / Child, Preschool / Cross Reactions / Female / Gryllidae / Humans / Immunoglobulin E / Male / Shellfish / Shellfish Hypersensitivity (Link to Publication Site) ● Publication site (DOI): 10.1016/j.molimm.2018.12.015 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30597474 ● Search Scopus @ Elsevier (PMID): 30597474 ● Search Scopus @ Elsevier (DOI): 10.1016/j.molimm.2018.12.015 (DOI: 10.1016/j.molimm.2018.12.015, PubMed: 30597474)
4.	Risa Ueta, Chihiro Abe, Ryosuke Ishihara, Takahito Watanabe, Sigeo Sugano, Hiroshi Ezura, Yuriko Osakabe and Keishi Osakabe : Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9., Scientific Reports, Vol.7, 507, 2017. (Summary) Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit-a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique. (Tokushima University Institutional Repository) ● Metadata: 110164 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-017-00501-4 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28360425 ● Summary page in Scopus @ Elsevier: 2-s2.0-85016547132 (Tokushima University Institutional Repository: 110164, DOI: 10.1038/s41598-017-00501-4, PubMed: 28360425, Elsevier: Scopus)
5.	Yuriko Osakabe, Takahito Watanabe, SS Sugano, R Ueta, R Ishihara, K Shinozaki and Keishi Osakabe : Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants., Scientific Reports, Vol.6, 26685, 2016. (Summary) Genome editing using the CRISPR/Cas9 system can be used to modify plant genomes, however, improvements in specificity and applicability are still needed in order for the editing technique to be useful in various plant species. Here, using genome editing mediated by a truncated gRNA (tru-gRNA)/Cas9 combination, we generated new alleles for OST2, a proton pump in Arabidopsis, with no off-target effects. By following expression of Cas9 and the tru-gRNAs, newly generated mutations in CRIPSR/Cas9 transgenic plants were detected with high average mutation rates of up to 32.8% and no off-target effects using constitutive promoter. Reducing nuclear localization signals in Cas9 decreased the mutation rate. In contrast, tru-gRNA Cas9 cassettes driven by meristematic- and reproductive-tissue-specific promoters increased the heritable mutation rate in Arabidopsis, showing that high expression in the germ line can produce bi-allelic mutations. Finally, the new mutant alleles obtained for OST2 exhibited altered stomatal closing in response to environmental conditions. These results suggest further applications in molecular breeding to improve plant function using optimized plant CRISPR/Cas9 systems. (Tokushima University Institutional Repository) ● Metadata: 110151 (Link to Publication Site) ● Publication site (DOI): 10.1038/srep26685 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27226176 ● Summary page in Scopus @ Elsevier: 2-s2.0-84971280492 (Tokushima University Institutional Repository: 110151, DOI: 10.1038/srep26685, PubMed: 27226176, Elsevier: Scopus)
6.	Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji and Taro Mito : TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis., Proceedings of the National Academy of Sciences of the United States of America, Vol.113, No.20, 5634-5639, 2016. (Summary) Although butterflies undergo a dramatic morphological transformation from larva to adult via a pupal stage (holometamorphosis), crickets undergo a metamorphosis from nymph to adult without formation of a pupa (hemimetamorphosis). Despite these differences, both processes are regulated by common mechanisms that involve 20-hydroxyecdysone (20E) and juvenile hormone (JH). JH regulates many aspects of insect physiology, such as development, reproduction, diapause, and metamorphosis. Consequently, strict regulation of JH levels is crucial throughout an insect's life cycle. However, it remains unclear how JH synthesis is regulated. Here, we report that in the corpora allata of the cricket, Gryllus bimaculatus, Myoglianin (Gb'Myo), a homolog of Drosophila Myoglianin/vertebrate GDF8/11, is involved in the down-regulation of JH production by suppressing the expression of a gene encoding JH acid O-methyltransferase, Gb'jhamt In contrast, JH production is up-regulated by Decapentaplegic (Gb'Dpp) and Glass-bottom boat/60A (Gb'Gbb) signaling that occurs as part of the transcriptional activation of Gb'jhamt Gb'Myo defines the nature of each developmental transition by regulating JH titer and the interactions between JH and 20E. When Gb'myo expression is suppressed, the activation of Gb'jhamt expression and secretion of 20E induce molting, thereby leading to the next instar before the last nymphal instar. Conversely, high Gb'myo expression induces metamorphosis during the last nymphal instar through the cessation of JH synthesis. Gb'myo also regulates final insect size. Because Myo/GDF8/11 and Dpp/bone morphogenetic protein (BMP)2/4-Gbb/BMP5-8 are conserved in both invertebrates and vertebrates, the present findings provide common regulatory mechanisms for endocrine control of animal development. (Link to Publication Site) ● Publication site (DOI): 10.1073/pnas.1600612113 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27140602 ● Summary page in Scopus @ Elsevier: 2-s2.0-84969765307 (DOI: 10.1073/pnas.1600612113, PubMed: 27140602, Elsevier: Scopus)
7.	Hiroko Awata, Takahito Watanabe, Yoshitaka Hamanaka, Taro Mito, Sumihare Noji and Makoto Mizunami : Knockout crickets for the study of learning and memory: Dopamine receptor Dop1 mediates aversive but not appetitive reinforcement in crickets, Scientific Reports, Vol.5, 15885, 2015. (Summary) Elucidation of reinforcement mechanisms in associative learning is an important subject in neuroscience. In mammals, dopamine neurons are thought to play critical roles in mediating both appetitive and aversive reinforcement. Our pharmacological studies suggested that octopamine and dopamine neurons mediate reward and punishment, respectively, in crickets, but recent studies in fruit-flies concluded that dopamine neurons mediates both reward and punishment, via the type 1 dopamine receptor Dop1. To resolve the discrepancy between studies in different insect species, we produced Dop1 knockout crickets using the CRISPR/Cas9 system and found that they are defective in aversive learning with sodium chloride punishment but not appetitive learning with water or sucrose reward. The results suggest that dopamine and octopamine neurons mediate aversive and appetitive reinforcement, respectively, in crickets. We suggest unexpected diversity in neurotransmitters mediating appetitive reinforcement between crickets and fruit-flies, although the neurotransmitter mediating aversive reinforcement is conserved. This study demonstrates usefulness of the CRISPR/Cas9 system for producing knockout animals for the study of learning and memory. (Tokushima University Institutional Repository) ● Metadata: 114966 (Link to Publication Site) ● Publication site (DOI): 10.1038/srep15885 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26521965 ● Search Scopus @ Elsevier (PMID): 26521965 ● Search Scopus @ Elsevier (DOI): 10.1038/srep15885 (Tokushima University Institutional Repository: 114966, DOI: 10.1038/srep15885, PubMed: 26521965)
8.	Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadic and Taro Mito : Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes., Biology Open, Vol.4, No.6, 702-709, 2015. (Summary) In insect species that undergo long germ segmentation, such as Drosophila, all segments are specified simultaneously at the early blastoderm stage. As embryogenesis progresses, the expression boundaries of Hox genes are established by repression of gap genes, which is subsequently replaced by Polycomb group (PcG) silencing. At present, however, it is not known whether patterning occurs this way in a more ancestral (short germ) mode of embryogenesis, where segments are added gradually during posterior elongation. In this study, two members of the PcG family, Enhancer of zeste (E(z)) and Suppressor of zeste 12 (Su(z)12), were analyzed in the short germ cricket, Gryllus bimaculatus. Results suggest that although stepwise negative regulation by gap and PcG genes is present in anterior members of the Hox cluster, it does not account for regulation of two posterior Hox genes, abdominal-A (abd-A) and Abdominal-B (Abd-B). Instead, abd-A and Abd-B are predominantly regulated by PcG genes, which is the mode present in vertebrates. These findings suggest that an intriguing transition of the PcG-mediated silencing of Hox genes may have occurred during animal evolution. The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains. Then, during insect evolution, the repression by transcription factors may have been acquired in anterior Hox genes of short germ insects, while PcG silencing was maintained in posterior Hox genes. (Tokushima University Institutional Repository) ● Metadata: 114742 (Link to Publication Site) ● Publication site (DOI): 10.1242/bio.201411064 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25948756 ● Search Scopus @ Elsevier (PMID): 25948756 ● Search Scopus @ Elsevier (DOI): 10.1242/bio.201411064 (Tokushima University Institutional Repository: 114742, DOI: 10.1242/bio.201411064, PubMed: 25948756)
9.	Takahito Watanabe, Sumihare Noji and Taro Mito : Gene knockout by targeted mutagenesis in a hemimetabolous insect, the two-spotted cricket Gryllus bimaculatus, using TALENs., Methods, Vol.69, No.1, 17-21, 2014. (Summary) Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically basal. These insects include many deleterious species. The cricket, Gryllus bimaculatus, is an emerging model for hemimetabolous insects, based on the success of RNA interference (RNAi)-based gene-functional analyses and transgenic technology. Taking advantage of genome-editing technologies in this species would greatly promote functional genomics studies. Genome editing using transcription activator-like effector nucleases (TALENs) has proven to be an effective method for site-specific genome manipulation in various species. TALENs are artificial nucleases that are capable of inducing DNA double-strand breaks into specified target sequences. Here, we describe a protocol for TALEN-based gene knockout in G. bimaculatus, including a mutant selection scheme via mutation detection assays, for generating homozygous knockout organisms. (Keyword) Animals / Deoxyribonucleases / Gene Knockout Techniques / Gryllidae / Homozygote / Mutagenesis, Site-Directed (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ymeth.2014.05.006 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24874787 ● Summary page in Scopus @ Elsevier: 2-s2.0-84929509568 (DOI: 10.1016/j.ymeth.2014.05.006, PubMed: 24874787, Elsevier: Scopus)

Proceeding of International Conference:

1.	Yuriko Osakabe, Kira Nozomu, Ueta Risa, Sakamoto Hideki, Takahito Watanabe, Takayanagi Eiko, Hara Chihiro, Hashimoto Ryosuke, Kohji Yamada and Keishi Osakabe : Development of in planta-regeneration system for plant genome editing, Frontiers in Genome Engineering 2019, Kobe Convention Center, Nov. 25-27, 2019, Nov. 2019.
2.	Risa Ueta, Chihiro Abe, Ryosuke Ishihara, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : Site-directed mutagenesis of the tomato IAA9 gene by using the CRISPR/Cas9 system, Latest Advances in Plant Development and Environmental Response 2016, CSH - Asia Meetings, Nov. 2016.
3.	Yuriko Osakabe, Risa Ueta, Sigeo Sugano, Takahito Watanabe, Kazuo Shinozaki and Keishi Osakabe : Genetic Engineering of Abiotic Stress Response in Plants, 3rd Conference of Cereal Biotechnology and Breeding, Nov. 2015.
4.	Matsuoka Yuji, Takahito Watanabe, Sayuri Tomonari, Sumihare Noji and Taro Mito : Functional analysis of a Hox gene, abdominal-A, using CRISPR/Cas9 system in the cricket Gryllus bimaculatus, International Tribolium Meeting 2015, Berkeley, USA, Aug. 2015.
5.	Takahito Watanabe, Matsuoka Yuji, Sayuri Tomonari, Kurita Chinami, Sumihare Noji and Taro Mito : Genome editing in the two-spotted cricket, Gryllus bimaculatus, using CRISPR/Cas9 system, Insect Genetic Technologies Workshop, Manhattan, Kansas, USA, Jun. 2015.
6.	Taro Mito, Itoh Takehiko, Morimoto Hiroya, Kajitani Ray, Toyoda Atsushi, Sayuri Tomonari, Fuketa Masao, Takahito Watanabe, Matsuoka Yuji and Sumihare Noji : Genome sequencing and annotation of the cricket Gryllus bimaculatus, a hemimetabolous insect model, Ninth Annual Arthropod Genomics Symposium, Manhattan, Kansas, USA, Jun. 2015.
7.	Sumihare Noji, Taro Mito, Bando Tetsuya, Nakamura Taro, Takahito Watanabe, Ishimaru Yoshiyasu and Hideyo Ohuchi : Regeneration of insect legs from stem cells, Thirteenth International Congress on Invertebrate Reproduction and Development, Detroit, Detroit, MI, USA, Jul. 2014.
8.	Takahito Watanabe, Sumihare Noji and Taro Mito : Targeted genome modifications in the cricket, Gryllus bimaculatus, using CRISPR/Cas9 system, International Symposium on RNAi and Genome editing methods, Tokushima, Japan, Mar. 2014.
9.	Taro Mito, Takahito Watanabe and Sumihare Noji : Genome modification technology in the cricket Gryllus bimaculatus, 1st Asian Invertebrate Immunity Symposium, Busan, Feb. 2014.
10.	Takahito Watanabe, Matsuoka Yuji, Sumihare Noji and Taro Mito : Targeted genome editing in the cricket, Gryllus bimaculatus, using CRISPR/Cas9 system, FASEB SRC on Genome Engineering-Cutting-Edge Research and Applications, Nassau, Bahamas, Jan. 2014.
11.	Takahito Watanabe, Ochiai Hiroshi, Sakuma Tetsushi, Ishihara Satoshi, Nakamura Taro, Yamamoto Takashi, Sumihare Noji and Taro Mito : Targeted genome modifications in the cricket, Gryllus bimaculatus, Conference of Transposition & Genome Engineering 2013, Budapest, Hungary, Sep. 2013.

Proceeding of Domestic Conference:

1.	吉良望, 高柳栄子, Takahito Watanabe, 坂本秀樹, Chihiro Hara, Ryosuke Hashimoto, Risa Ueta, Yuriko Osakabe, Yuriko Osakabe and Keishi Osakabe : トマトゲノム編集のためのin planta-regeneration法の開発, 第37回日本植物細胞分子生物学会大会,京都府立大学, 2019年9月7日-8日, Sep. 2019.
2.	吉良望, 高柳栄子, Takahito Watanabe, Risa Ueta, Takahito Watanabe, Chihiro Hara, Ryosuke Hashimoto, Yuriko Osakabe and Keishi Osakabe : トマトゲノム編集のためのin planta-regeneration法の開発, 日本ゲノム編集学会第4回大会, タワーホール船堀(東京), 2019年6月4日-5日, Jun. 2019.
3.	Kohei Kawamoto, Mayuko Matsuda, Takahisa Yamashita, Takahito Watanabe, Sayuri Tomonari, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Precise in-frame integration of a GFP gene using microhomology-mediated knock-in technology in Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
4.	Takahisa Yamashita, Taro Mito, Yoshiyasu Ishimaru, Takahito Watanabe, Sayuri Tomonari, Kohei Kawamoto and Mayuko Matuda : Generation of an enhancer-trap strain of the scalloped gene in the cricket Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
5.	Yuki Nakamura, Sayuri Tomonari, Kohei Kawamoto, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Resolving the correlation between phenotype and genotype in a segmentation gene even-skipped in the cricket Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
6.	吉良望, Risa Ueta, Takahito Watanabe, 高柳栄子, 坂本秀樹, Chihiro Abe, Ryosuke Hashimoto, Yuriko Osakabe and Keishi Osakabe : Development of in planta-regeneration system for genome editing in tomato, 第60回日本植物生理学会年会, 名古屋大学, 2019年3月13日-15日, Mar. 2019.
7.	吉良望, 高柳栄子, 坂本秀樹, Takahito Watanabe, Chihiro Abe, Ryosuke Hashimoto, Yuriko Osakabe and Keishi Osakabe : トマト茎頂組織への新規in planta 遺伝子導入法の開発, 第36回日本植物分子生物学会(金沢)大会, 金沢商工会議所会館, 2018年8月26∼28日, Aug. 2018.
8.	吉良望, 高柳栄子, 坂本秀樹, Takahito Watanabe, Chihiro Abe, Ryosuke Hashimoto, Yuriko Osakabe and Keishi Osakabe : トマト茎頂組織への新規in planta 遺伝子導入法の開発, 日本ゲノム編集学会第3回大会, 広島国際会議場(広島市), 2018年6月16日-20日, Jun. 2018.
9.	Yu-ki Nakamura, Ko-hei Kawamoto, Sayuri Tomonari, Takahito Watanabe, Yoshiyasu Ishimaru, Taro Mito and Sumihare Noji : Gene knock-out analysis of a segmentation gene even-skipped in the cricket Gryllus bimaculatus, Joint Annual Meeting of JSDB 51st and JSCB 70th, Jun. 2018.
10.	吉良望, 高柳栄子, 坂本秀樹, Takahito Watanabe, 阿部千尋, 橋本諒典, Yuriko Osakabe and Keishi Osakabe : In planta genome editing in tomato meristem tissues, 第59回日本植物生理学会年会, Mar. 2018.
11.	上村菜月, Sayuri Tomonari, Takahito Watanabe, 松岡佑児, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : 遺伝子ノックイン技術の応用によるレポーターコオロギ系統の作製, 第88回日本動物学会, Sep. 2017.
12.	上田梨紗, 阿部千尋, 橋本諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : トマトの機能改変を目指した高効率ゲノム編集技術の確立, 日本ゲノム編集学会第2回大会, 千里ライフサイエンスセンター, 大阪, Jun. 2017.
13.	Matsuda Mayuko, Matsuoka Yuji, Yoshiyasu Ishimaru, Sayuri Tomonari, Takahito Watanabe, Sumihare Noji and Taro Mito : Functional analysis of a Hox gene, abdominal-A, in the cricket Gryllus bimaculatus using a CRISPR/Cas9-mediated gene knock-in system, Japanese Society of Developmental Biologists, May 2017.
14.	Nakamura Yu-Ki, Kawamoto Kohei, Sayuri Tomonari, Matsuda Mayuko, Takahito Watanabe, Yoshiyasu Ishimaru, Uemura Natsuki, Sumihare Noji and Taro Mito : even-skipped is required for segmentation and elongation of embryos in the cricket Gryllus bimaculatusas revealed by CRISPR/Cas9-based gene knock-out., Japanese Society of Developmental Biologists, May 2017.
15.	Masamitsu Takai, Takahito Watanabe, Sayuri Tomonari and Taro Mito : フタホシコオロギの食用化に向けた生産システムの検討, 第61回日本応用動物昆虫学会大会, Mar. 2017.
16.	阿部千尋, 上田梨紗, 橋本諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : 栽培品種トマトAilsa Craigの CRISPR/Cas9システムを用いた新育種技術開発, 第58回日本植物生理学会年会, Mar. 2017.
17.	Risa Ueta, Chihiro Abe, Ryosuke Ishihara, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9, 第58回日本植物生理学会年会, Mar. 2017.
18.	阿部千尋, 上田梨紗, 橋本諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : 栽培品種トマトAilsa Craigの CRISPR/Cas9システムを用いた新育種技術開発, 日本生物工学会西日本支部第3回講演会, Dec. 2016.
19.	上田梨紗, 阿部千尋, 橋本諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9遺伝子を標的としたゲノム編集技術の確立, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
20.	坂本秀樹, Takahito Watanabe, 上田梨紗, 島田佳南里, 福原真樹, Yuriko Osakabe and Keishi Osakabe : 電気穿孔を用いた直接導入法およびin planta法による植物ゲノム編集技術の開発, 第39回日本分子生物学会年会, Nov. 2016.
21.	上田梨紗, 阿部千尋, 石原諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9遺伝子を標的としたゲノム編集技術の確立, 日本植物学会第80回大会, Sep. 2016.
22.	Yuriko Osakabe, Takahito Watanabe, Sigeo Sugano, 上田梨紗, 石原諒典, 篠崎一雄 and Keishi Osakabe : ゲノム編集技術による植物環境応答能の改変, 日本ゲノム編集学会第1回大会, Sep. 2016.
23.	阿部千尋, 上田梨紗, 石原諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : 栽培品種トマトAilsa Craigの CRISPR/Cas9システムを用いた新育種技術開発, 日本ゲノム編集学会第1回大会, Sep. 2016.
24.	上田梨紗, 阿部千尋, 石原諒典, Takahito Watanabe, Sigeo Sugano, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9遺伝子を標的としたゲノム編集技術の確立, 日本ゲノム編集学会第1回大会, Sep. 2016.
25.	Kohei Kawamoto, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : even-skipped acts principally as a gap gene in the cricket Gryllus bimaculatus as revealed by CRISPR/Cas9-based gene knockout analysis, JSDB Special Symposium: Frontier of Developmental Biology Hosted by JSDB, Jun. 2016.
26.	上田梨紗, 阿部千尋, 石原諒典, Takahito Watanabe, Sigeo Sugano, Katsuyuki Miyawaki, Sumihare Noji, Yuriko Osakabe and Keishi Osakabe : 高効率 CRISPR/Cas9による SlIAA9 ノックアウトトマトの作出, 第57回日本植物生理学会大会, Mar. 2016.
27.	Yuriko Osakabe, Sigeo Sugano, Takahito Watanabe, 上田梨紗, 石原諒典, 篠崎一雄 and Keishi Osakabe : CRISPR/Cas9によるシロイヌナズナ環境ストレス応答性遺伝子のゲノム編集, 第57回日本植物生理学会大会, Mar. 2016.
28.	上田梨紗, 石原諒典, 阿部千尋, Takahito Watanabe, Sigeo Sugano, Katsuyuki Miyawaki, Sumihare Noji, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9 遺伝子を標的としたゲノム編集技術の確立, 第38回日本分子生物学会年会, Dec. 2015.
29.	Sayuri Tomonari, 川本晃平, 松岡佑児, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : CRISPR/Cas9システムを用いた遺伝子ノックアウトによるコオロギ胚発生制御メカニズムの解析, 第38回日本分子生物学会年会, Dec. 2015.
30.	Yuji Matsuoka, Takahito Watanabe, 栗田千波, Sayuri Tomonari, Sumihare Noji and Taro Mito : フタホシコオロギにおけるCRISPR/Cas9システムを用いたHox遺伝子abdominal-Aの機能解析, 第48回日本発生生物学会, Jun. 2015.
31.	上田梨紗, 石原諒典, Takahito Watanabe, 菅野茂夫, 宮脇克行, 野地澄晴, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9遺伝子を標的としたゲノム編集技術の確立, 第56回日本植物生理学会年会, Mar. 2015.
32.	Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Sumihare Noji and Taro Mito : Functions of Polycomb group gene in regulation of Hox gene expression in a primitive mode of insect embryogenesis in the cricket Gryllus bimaculatus, 第47回日本発生生物学会, May 2014.
33.	Takahito Watanabe, Yuji Matsuoka, Taro Mito and Sumihare Noji : Targeted gene disruption in the cricket, Gryllus bimaculatus, using CRISPR/Cas9 system, 第47回日本発生生物学会, May 2014.
34.	平田翔悟, 上田梨紗, Takahito Watanabe, Katsuyuki Miyawaki, 三戸太郎 and 野地澄晴 : Transcription Activator-Like Effector NucleasesによるIAA9ノックアウトトマト作製の試み, 第36回日本分子生物学会, Dec. 2013.

Et cetera, Workshop:

1.	Aya Nakai, koji Murai, Akihito Tanaka, Hiroshi Kondoh, Sugina Watanabe, Akiko Uebansou, Takahito Watanabe and Katsuyuki Miyawaki : LED植物工場を活用した藍の生育と二次代謝物質生産の制御, LED総合フォーラム2019 in徳島, 63-64, Feb. 2019.
2.	Akihiro Yasue, Silvia Naomi Mitsui Akagi, Takahito Watanabe, 佐久間哲史, Seiichi Oyadomari, 山本卓, Sumihare Noji, Taro Mito and Eiji Tanaka : TALEN，CRISPR/Casシステムを用いたマウス1細胞期胚における標的遺伝子破壊, 第3回ゲノム編集研究会, Oct. 2013.

Patent:

1.	Takahito Watanabe, Taro Mito, 河合重和, 村田康弘 and 松本崇 : 飼育装置, 2020-52445 (Mar. 2020), .
2.	Takahito Watanabe and Taro Mito : コオロギの育成装置及び育成方法, 2020-019352 (Feb. 2020), .
3.	Taro Mito and Takahito Watanabe : 遺伝子改変不完全変態昆虫の作製方法, 2017-196367 (Oct. 2017), 2019-068762 (May 2019), JP2019-068762A (May 2019).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Analysis of mechanisms of regeneration-bud formation in the cricket using novel genetic engineering method (Project/Area Number: 16K21199 )
Analysis of mechanisms of wing development in hemimetabolous insect using conditional knock-out method. (Project/Area Number: 26870415 )
Study on genomic basis of morphogenesis in hemimetabolous insects using novel genetic modification techniques (Project/Area Number: 26292176 )
Search by Researcher Number (30709481)

Assistant Professor : Watanabe, Takahito

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)