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Academic Paper (Judged Full Paper):

1. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.,
The Journal of reproduction and development, 2024.
(DOI: 10.1262/jrd.2024-054,   PubMed: 39218670)
2. Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Fuminori Tanihara, Takeshige Otoi and Maki Hirata :
Effects of ergothioneine supplementation on meiotic competence and porcine oocyte development.,
Veterinary world, Vol.17, No.8, 1748-1752, 2024.
(DOI: 10.14202/vetworld.2024.1748-1752,   PubMed: 39328438)
3. Bin Liu, Manita Wittayarat, Koki Takebayashi, Qingyi Lin, Nanaka Torigoe, Zhao Namula, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Effects of centrifugation treatment before electroporation on gene editing in pig embryos.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00926-y,   PubMed: 38833208)
4. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00908-0,   PubMed: 38664280)
5. Thanh-Van Nguyen, Kim Lanh Thi Do, Qingyi Lin, Megumi Nagahara, Zhao Namula, Manita Wittayarat, Maki Hirata, Takeshige Otoi and Fuminori Tanihara :
Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00869-4,   PubMed: 38485817)
6. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos.,
Animal Science Journal, Vol.94, No.1, e13878, 2023.
(DOI: 10.1111/asj.13878,   PubMed: 37818780)
7. Chommanart Thongkittidilok, Maki Hirata, Qingyi Lin, Nanaka Torigoe, Bin Liu, Yoko Sato, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study.,
Veterinary Medicine International, Vol.2023, 7000858, 2023.
(Tokushima University Institutional Repository: 119494,   DOI: 10.1155/2023/7000858,   PubMed: 37609627,   Elsevier: Scopus)
8. Fuminori Tanihara, Maki Hirata, Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Qingyi Lin, Koki Takebayashi, Hiromasa Hara, Megumi Nagahara and Takeshige Otoi :
GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system.,
Molecular Biology Reports, Vol.50, No.6, 5049-5057, 2023.
(DOI: 10.1007/s11033-023-08388-3,   PubMed: 37101010)
9. Thanh-Van Nguyen, Kim Lanh Thi Do, Zhao Namula, Qingyi Lin, Nanaka Torigoe, Megumi Nagahara, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Development and Genome Mutation of Bovine Zygotes Vitrified Before and After Genome Editing via Electroporation,
Cryo Letters, Vol.44, No.2, 118-122, 2023.
(DOI: 10.54680/fr23210110612,   Elsevier: Scopus)
10. Fuminori Tanihara, Maki Hirata, Zhao Namula, Kim Lanh Thi Do, Naoaki Yoshimura, Qingyi Lin, Koki Takebayashi, Tetsushi Sakuma, Takashi Yamamoto and Takeshige Otoi :
Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN,
Frontiers in Cell and Developmental Biology, Vol.11, 2023.
(DOI: 10.3389/fcell.2023.884340,   PubMed: 36711037)
11. Anh Quynh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Kim Lanh Thi Do and Takeshige Otoi :
Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.,
Veterinary World, Vol.15, No.9, 2210-2216, 2022.
(Tokushima University Institutional Repository: 117906,   DOI: 10.14202/vetworld.2022.2210-2216,   PubMed: 36341066)
12. Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Maki Hirata, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Kim Lanh Thi Do, Fuminori Tanihara and Takeshige Otoi :
Gene editing in porcine embryos using a combination of electroporation and transfection methods.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.10, 1136-1142, 2022.
(DOI: 10.1111/rda.14184,   PubMed: 35699358)
13. Megumi Shimazaki, Manita Wittayarat, Rentsenkhand Sambuu, Asami Sugita, Masaki Kawaguchi, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi, Takeshige Otoi and Yoko Sato :
Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle-yaks affects spermatogenic cell fate and sterility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.9, 999-1006, 2022.
(DOI: 10.1111/rda.14166,   PubMed: 35614560,   Elsevier: Scopus)
14. Asami Okada, Misuzu Yamada-Yamashita, Yukari Tominaga, Kyoka Jo, Hiroyasu Mori, Reiko Suzuki, Masashi Ishizu, Motoyuki Tamaki, Yuko Akehi, Yuichi Takashi, Daisuke Koga, Eisuke Shimokita, Fuminori Tanihara, Kiyoe Kurahashi, Sumiko Yoshida, Yukari Mitsui, Shiho Masuda, Itsuro Endo, Ken-ichi Aihara, Shoji Kagami, Masahiro Abe, Kevin Ferreri, Yoshio Fujitani, Munehide Matsuhisa and Akio Kuroda :
Novel method utilizing bisulfite conversion with dual amplification-refractory mutation system polymerase chain reaction to detect circulating pancreatic β-cell cfDNA.,
Journal of Diabetes Investigation, Vol.13, No.7, 1140-1148, 2022.
(Tokushima University Institutional Repository: 117206,   DOI: 10.1111/jdi.13806,   PubMed: 35396829)
15. Zhao Namula, Manita Wittayarat, Kim Lanh Thi Do, Thanh Nguyen Van, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system,
Veterinary and Animal Science, Vol.16, 2022.
(Tokushima University Institutional Repository: 117723,   DOI: 10.1016/j.vas.2022.100241,   PubMed: 35265771)
16. Zhao Namula, Anh Quynh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Kim Lanh Thi Do, Fuminori Tanihara and Takeshige Otoi :
Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection.,
Veterinary World, Vol.15, No.2, 496-501, 2022.
(Tokushima University Institutional Repository: 117724,   DOI: 10.14202/vetworld.2022.496-501,   PubMed: 35400948)
17. Qingyi Lin, Anh Quynh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.5, 556-563, 2022.
(DOI: 10.1111/rda.14095,   PubMed: 35137478)
18. Qingyi Lin, Anh Quynh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
Short-term preservation of porcine zygotes at ambient temperature using a chemically defined medium.,
Animal Science Journal, Vol.93, No.1, 2022.
(DOI: 10.1111/asj.13711,   PubMed: 35373427,   CiNii: 1360580232188129664,   Elsevier: Scopus)
19. Chommanart Thongkittidilok, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Lanh Thi Kim Do, Zhao Namula, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Effects of individual or in-combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.3, 314-320, 2021.
(DOI: 10.1111/rda.14063,   PubMed: 34862995)
20. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.,
International Journal of Molecular Sciences, Vol.22, No.5, 2249, 2021.
(Tokushima University Institutional Repository: 116340,   DOI: 10.3390/ijms22052249,   PubMed: 33668187,   Elsevier: Scopus)
21. Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Le Anh, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara and Takeshige Otoi :
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.,
Animals : An Open Access Journal from MDPI, Vol.11, No.2, 2021.
(Tokushima University Institutional Repository: 116270,   DOI: 10.3390/ani11020578,   PubMed: 33672168,   Elsevier: Scopus)
22. Anh Quynh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata and Takeshige Otoi :
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.,
BMC Research Notes, Vol.14, No.1, 7, 2021.
(Tokushima University Institutional Repository: 116277,   DOI: 10.1186/s13104-020-05412-8,   PubMed: 33407863)
23. Kim Thi Lanh Do, Manita Wittayarat, Yoko Sato, Kaywalee Chatdarong, Theerawat Tharasanit, Mongkol Techakumphu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Comparison of blastocyst development between cat-cow and cat-pig interspecies somatic cell nuclear transfer embryos under the treatment of Trichostatin A.,
Biology bulletin of the Russian Academy of Sciences, Vol.48, 107-117, 2021.
(DOI: 10.1134/S1062359021020035)
24. Nguyen Nhien Thi, Wittayarat Manita, Zhao Namula, Sato Yoko, Anh Le Quynh, Lin Qingyi, Takebayashi Koki, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Chlorogenic acid and insulin-transferrin-selenium supplementation during in vitro maturation enhances the developmental competence of interspecies chimera blastocysts following cell injection.,
Journal of Applied Animal Research, Vol.49, No.1, 486-491, 2021.
(DOI: 10.1080/09712119.2021.2011295,   Elsevier: Scopus)
25. Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, T Nhien Nguyen, A Quynh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.,
Animal Science Journal, Vol.92, No.1, e13534, 2021.
(DOI: 10.1111/asj.13534,   PubMed: 33638256)
26. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Le Anh Quynh, Manita Wittayarat, Mokhamad Fahrudin, Takayuki Hirano and Takeshige Otoi :
Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes.,
Animal Biotechnology, Vol.32, 147-154, 2021.
(DOI: 10.1080/10495398.2019.1668801,   PubMed: 31558095)
27. Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi and Takeshige Otoi :
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.8, 614-621, 2020.
(DOI: 10.1007/s11626-020-00507-9,   PubMed: 32978715)
28. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Thi Nhien Nguyen, Koki Takebayashi, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation.,
Molecular Biology Reports, Vol.47, No.7, 5073-5079, 2020.
(DOI: 10.1007/s11033-020-05576-3,   PubMed: 32519310)
29. Takayuki Hirano, Maki Hirata, Shigeyuki Fujimoto, Thi Nhien Nguyen, Le Anh Quynh, Fuminori Tanihara and Takeshige Otoi :
Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.4, 277-280, 2020.
(DOI: 10.1007/s11626-020-00451-8,   PubMed: 32394241)
30. Fuminori Tanihara, Maki Hirata, Nhien Nguyen Thi, Le Anh Quynh, Takayuki Hirano and Takeshige Otoi :
Generation of viable PDX1 gene-edited founder pigs as providers of nonmosaics.,
Molecular Reproduction and Development, Vol.87, No.4, 471-481, 2020.
(DOI: 10.1002/mrd.23335,   PubMed: 32166879)
31. T N Nguyen, Maki Hirata, Fuminori Tanihara, Y Sato, Z Namula, Le Trong Quang, M Wittayarat, M Fahrudin and Takeshige Otoi :
In vitro Development of Zona Pellucida-free Porcine Zygotes Cultured Individually after Vitrification.,
Cryo Letters, Vol.41, No.2, 86-91, 2020.
(PubMed: 33988658)
32. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi :
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020.
(DOI: 10.1111/rda.13609,   PubMed: 31858644)
33. Namula Zhao, Yoko Sato, Manita Wittayarat, Quynh Le Anh, Thi Nhien Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Curcumin supplementation in maturation medium improves the maturation, fertilisation, and developmental competence of porcine oocytes.,
Acta Veterinaria Hungarica, Vol.68, No.3, 298-304, 2020.
(DOI: 10.1556/004.2020.00041,   PubMed: 33221737)
34. A Quynh Le, Maki Hirata, T Nhien Nguyen, Koki Takebayashi, Manita Wittayarat, Yoko Sato, Zhao Namula, Masahiro Nii, Fuminori Tanihara and Takeshige Otoi :
Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes.,
Animal Science Journal, Vol.91, No.1, e13386, 2020.
(DOI: 10.1111/asj.13386,   PubMed: 32512638)
35. Maki Hirata, Manita Wittayarat, Takayuki Hirano, Thi Nhien Nguyen, Le Anh Quynh, Zhao Namula, Mokhamad Fahrudin, Fuminori Tanihara and Takeshige Otoi :
The relationship between embryonic development and the efficiency of target mutations in porcine endogenous retroviruses (PERVs) pol genes in porcine embryos.,
Animals : An Open Access Journal from MDPI, Vol.9, No.9, E593, 2019.
(Tokushima University Institutional Repository: 114984,   DOI: 10.3390/ani9090593,   PubMed: 31443357)
36. Namula Zhao, Manita Wittayarat, Maki Hirata, Hirano Takayuki, Nguyen Thi Nhien, Le Anh Quynh, Fahrudin Mokhamad, Fuminori Tanihara and Takeshige Otoi :
Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.8, 598-603, 2019.
(DOI: 10.1007/s11626-019-00385-w,   PubMed: 31297696)
37. Fuminori Tanihara, Maki Hirata, Morikawa Shigeki, Thi Nhien Nguyen, Le Anh Quynh, Hirano Takayuki, Fukumi Yoshiyuki, Abe Toshiaki and Takeshige Otoi :
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts,
The Journal of Reproduction and Development, Vol.65, No.5, 475-479, 2019.
(Tokushima University Institutional Repository: 115540,   DOI: 10.1262/jrd.2019-049,   PubMed: 31178553)
38. Fuminori Tanihara, Maki Hirata, Satoru Iizuka, Shinya Sairiki, Masahiro Nii, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
Relationship among ovarian follicular status, developmental competence of oocytes, and anti-M llerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.,
Animal Science Journal, Vol.90, No.6, 712-718, 2019.
(DOI: 10.1111/asj.13200,   PubMed: 30977253)
39. Nhien Nguyen Thi, Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.54, No.5, 750-755, 2019.
(DOI: 10.1111/rda.13417,   PubMed: 30788874,   Elsevier: Scopus)
40. Zhao Namula, Fuminori Tanihara, Manita Wittayarat, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.,
Acta Veterinaria Hungarica, Vol.67, No.1, 106-114, 2019.
(DOI: 10.1556/004.2019.012,   PubMed: 30922097)
41. Maki Hirata, Fuminori Tanihara, Manita Wittayarat, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, Zhao Namula, Masahiro Nii and Takeshige Otoi :
Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.4, 237-242, 2019.
(DOI: 10.1007/s11626-019-00338-3,   PubMed: 30820813)
42. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh LE, Takayuki Hirano and Takeshige Otoi :
Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos.,
The Journal of Reproduction and Development, Vol.65, No.3, 209-214, 2019.
(Tokushima University Institutional Repository: 115541,   DOI: 10.1262/jrd.2018-116,   PubMed: 30726783,   Elsevier: Scopus)
43. Fuminori Tanihara, Maki Hirata, Nhien T. Nguyen, Quynh A. Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.,
Animal Science Journal, Vol.90, No.1, 55-61, 2018.
(DOI: 10.1111/asj.13129,   PubMed: 30368976)
44. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.,
PLoS ONE, Vol.13, No.10, e0206360, 2018.
(Tokushima University Institutional Repository: 112917,   DOI: 10.1371/journal.pone.0206360,   PubMed: 30352075)
45. Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Mitsuhiro Takagi, Tsukasa Terazono and Takeshige Otoi :
Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.,
Veterinary Medicine and Science, 2018.
(Tokushima University Institutional Repository: 115827,   DOI: 10.1002/vms3.121,   PubMed: 30273973)
46. Maki Hirata, Fuminori Tanihara, Taniguchi Masayasu, Takagi Mitsuhiro, Terazono Tsukasa and Takeshige Otoi :
Viability of canine ovaries autografted to different peripheral sites,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, No.2, 140-148, 2018.
(Elsevier: Scopus)
47. Zhao Namula, Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Nhien Nguyen Thi, Takayuki Hirano, Masahiro Nii and Takeshige Otoi :
Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.,
Reproduction in Domestic Animals = Zuchthygiene, 2018.
(Tokushima University Institutional Repository: 116076,   DOI: 10.1111/rda.13288,   PubMed: 30053311)
48. Tetsushi Ono, Mitsuhiro Takagi, Chiho Kawashima, B Missaka P Wijayagunawardane, M Peter L A Vos, Masayasu Taniguchi, Fuminori Tanihara and Takeshige Otoi :
Comparative Effects of Different Dosages of hCG on Follicular Development in Postpartum Dairy Cows With Cystic Ovarian Follicles.,
Frontiers in Veterinary Science, Vol.5, 2018.
(Tokushima University Institutional Repository: 114493,   DOI: 10.3389/fvets.2018.00130,   PubMed: 30009161)
49. Thanh-Van Nguyen, Manita Wittayarat, Kim Lanh Thi Do, Van Thanh Nguyen, Masahiro Nii, Zhao Namula, Toshiki Kunihara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.,
Animal Science Journal, Vol.89, No.8, 1207-1213, 2018.
(Tokushima University Institutional Repository: 116078,   DOI: 10.1111/asj.13049,   PubMed: 29806122)
50. Fuminori Tanihara, Maki Hirata, Thi Nguyen Nhien, Takayuki Hirano, Toshiki Kunihara and Takeshige Otoi :
Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation.,
The Journal of Veterinary Medical Science, Vol.80, No.6, 1007-1011, 2018.
(Tokushima University Institutional Repository: 115574,   DOI: 10.1292/jvms.18-0131,   PubMed: 29769451)
51. V T Nguyen, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Katsutoshi Nishio, T Do L Kim, V T Nguyen, M Nii and Takeshige Otoi :
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.,
Cryo Letters, Vol.39, No.2, 131-136, 2018.
(PubMed: 29734422,   Elsevier: Scopus)
52. Megumi Shimazaki, Urasoko Saki, Tanaka Masako, Sato Yoko, Fuminori Tanihara, Maki Hirata, Taniguchi Masayasu, Takagi Mitsuhiro and Takeshige Otoi :
Effects of Orvus Es Paste (OEP) on the viability of bull spermatozoa after double freezing and thawing.,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, 32-38, 2018.
(CiNii: 1010003825435470592,   Elsevier: Scopus)
53. Katsutoshi Nishio, Fuminori Tanihara, T-V Nguyen, Toshiki Kunihara, M Nii, Maki Hirata, Tatsuya Takemoto and Takeshige Otoi :
Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.53, No.2, 313-318, 2017.
(Tokushima University Institutional Repository: 116077,   DOI: 10.1111/rda.13106,   PubMed: 29135047)
54. T-V Nguyen, Fuminori Tanihara, Ltk Do, Y Sato, M Taniguchi, M Takagi, T Nguyen Van and Takeshige Otoi :
Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.52, No.6, 969-975, 2017.
(Tokushima University Institutional Repository: 116075,   DOI: 10.1111/rda.13005,   PubMed: 28660662)
55. Katsutoshi Nishio, Mado Yamazaki, Masayasu Taniguchi, Kazuhiko Besshi, Fumio Morita, Toshiki Kunihara, Fuminori Tanihara, Tatsuya Takemoto and Takeshige Otoi :
Sensitivity of the meiotic stage to hyperthermia during in vitro maturation of porcine oocytes.,
Acta Veterinaria Hungarica, Vol.65, No.1, 115-123, 2017.
(Tokushima University Institutional Repository: 116080,   DOI: 10.1556/004.2017.012,   PubMed: 28244334)
56. Fuminori Tanihara, Tatsuya Takemoto, Eri Kitagawa, Shengbin Rao, Kim Lanh Thi Do, Akira Onishi, Yukiko Yamashita, Chisato Kosugi, Hitomi Suzuki, Shoichiro Sembon, Shunichi Suzuki, Michiko Nakai, Masakazu Hashimoto, Akihiro Yasue, Munehide Matsuhisa, Sumihare Noji, Tatsuya Fujimura, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Somatic cell reprogramming-free generation of genetically modified pigs.,
Science Advances, Vol.2, No.9, 2016.
(Tokushima University Institutional Repository: 110154,   DOI: 10.1126/sciadv.1600803,   PubMed: 27652340)
57. L Do, M Wittayarat, T Terazono, Y Sato, M Taniguchi, Fuminori Tanihara, Tatsuya Takemoto, Y Kazuki, K Kazuki, M Oshimura and Takeshige Otoi :
Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.51, No.6, 1039-1043, 2016.
(DOI: 10.1111/rda.12766)
58. N Kurniani Karja Wayan, M Fahrudin, MA Setiadi, LI Tumbelaka, R Sudarwati, YT Hastuti, BH Mulia, A Widianti, K Sultan, T Terazono, Z Namula, M Taniguchi, Fuminori Tanihara, Tatsuya Takemoto, K Kikuchi, Y Sato and Takeshige Otoi :
Characteristics and fertility of sumatran tiger spermatozoa cryopreserved with different sugars.,
Cryo Letters, Vol.37, No.4, 264-271, 2016.
(PubMed: 27925009,   Elsevier: Scopus)
59. M Nakai, J Ito, N Kashiwazaki, NT Men, Fuminori Tanihara, J Noguchi, H Kaneko, A Onishi and K Kikuchi :
Treatment with protein kinase C activator is effective for improvement of male pronucleus formation and further embryonic development of sperm-injected oocytes in pigs,
Theriogenology, Vol.85, No.4, 703-708, 2016.
(DOI: 10.1016/j.theriogenology.2015.10.011,   PubMed: 26559470)
60. Morita Yasuhiro, Taniguchi Masayasu, Fuminori Tanihara, Ito Aya, Namula Zhao, DO Thi Kim Lanh, Takagi Mitsuhiro, Tatsuya Takemoto and Takeshige Otoi :
The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture,
The Journal of Veterinary Medical Science, 2016.
(Tokushima University Institutional Repository: 115575,   DOI: 10.1292/jvms.15-0658,   PubMed: 26947170)
61. Ono Tetsushi, Isobe Tomohiro, Morita Yasuhiro, Do Lanh Thi Kim, Fuminori Tanihara, Taniguchi Masayasu, Takagi Mitsuhiro and Takeshige Otoi :
Effects of parity and season on pregnancy rates after the transfer of embryos to repeat-breeder Japanese Black beef cattle.,
Archives Animal Breeding, Vol.59, 45-49, 2016.
(Tokushima University Institutional Repository: 117960,   DOI: 10.5194/aab-59-45-2016,   CiNii: 1360848665111816960,   Elsevier: Scopus)
62. Tetsushi Ono, Asako Takaoka, Yasuhiro Morita, Lanh Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus-oocyte complexes.,
Acta Veterinaria Hungarica, Vol.63, No.4, 485-498, 2015.
(DOI: 10.1556/004.2015.045,   PubMed: 26599095,   Elsevier: Scopus)
63. L T. K. Do, Y Shibata, M Taniguchi, M Nii, T V. Nguyen, Fuminori Tanihara, M Takagi and Takeshige Otoi :
Melatonin Supplementation During In Vitro Maturation and Development Supports the Development of Porcine Embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.50, No.6, 1054-1058, 2015.
(DOI: 10.1111/rda.12607,   PubMed: 26392209,   Elsevier: Scopus)
64. Yasuhiro Morita, Ni Wayan Kurniani Karja, Lanh Thi Kim Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.,
Animal Biotechnology, Vol.26, No.4, 273-275, 2015.
(DOI: 10.1080/10495398.2015.1015681,   PubMed: 26158458,   Elsevier: Scopus)
65. Megumi Shimazaki, Rentsenkhand Sambuu, Yoko Sato, Kim Lanh Thi Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.,
Cryo Letters, Vol.36, No.4, 264-269, 2015.
(PubMed: 26576001,   Elsevier: Scopus)
66. Tomohiro Isobe, Yoshihisa Ikebata, Lanh Thi Kim Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.,
Animal Science Journal, Vol.86, No.7, 661-665, 2014.
(DOI: 10.1111/asj.12341,   PubMed: 25488699)
67. Manita Wittayarat, Akira Fujiwara, Kaywalee Chatdarong, Mongkol Techakumphu, Yoko Sato, Fuminori Tanihara, Yasuhiro Morita, Masayasu Taniguchi and Takeshige Otoi :
Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors.,
Acta Veterinaria Hungarica, Vol.62, No.2, 233-242, 2014.
(DOI: 10.1556/AVet.2013.050,   PubMed: 24334073,   Elsevier: Scopus)
68. Tamás Somfai, Koji Yoshioka, Fuminori Tanihara, Hiroyuki Kaneko, Junko Noguchi, Naomi Kashiwazaki, Takashi Nagai and Kazuhiro Kikuchi :
Generation of live piglets from cryopreserved oocytes for the first time using a defined system for in vitro embryo production.,
PLoS ONE, Vol.9, No.5, e97731, 2014.
(DOI: 10.1371/journal.pone.0097731,   PubMed: 24844283,   Elsevier: Scopus)
69. Hiroyuki Kaneko, Kazuhiro Kikuchi, Fuminori Tanihara, Junko Noguchi, Michiko Nakai, Junya Ito and Naomi Kashiwazaki :
Normal reproductive development of pigs produced using sperm retrieved from immature testicular tissue cryopreserved and grafted into nude mice.,
Theriogenology, Vol.82, No.2, 325-331, 2014.
(DOI: 10.1016/j.theriogenology.2014.04.012,   PubMed: 24835638)
70. Zhao Namula, Risa Kodama, Fuminori Tanihara, Yasuhiro Morita, Yoko Sato, Manita Wittayarat, Masayasu Taniguchi and Takeshige Otoi :
Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C.,
Acta Veterinaria Hungarica, Vol.62, No.1, 106-116, 2014.
(DOI: 10.1556/AVet.2013.046,   PubMed: 24334075,   Elsevier: Scopus)
71. Fuminori Tanihara, Michiko Nakai, Nguyen Thi Men, Noriko Kato, Hiroyuki Kaneko, Junko Noguchi, Takeshige Otoi and Kazuhiro Kikuchi :
Roles of the zona pellucida and functional exposure of the sperm-egg fusion factor 'IZUMO' during in vitro fertilization in pigs.,
Animal Science Journal, Vol.85, No.4, 395-404, 2014.
(DOI: 10.1111/asj.12164,   PubMed: 24450993)
72. Fuminori Tanihara, Yukine Kaedei, Zhao Namula, Vien Viet Luu, Yoko Sato, Manita Wittayarat, Masayasu Taniguchi and Takeshige Otoi :
Comparison of activation ability between feline and bovine oocytes.,
Acta Veterinaria Hungarica, Vol.61, No.4, 491-494, 2013.
(DOI: 10.1556/AVet.2013.032,   PubMed: 23974933,   Elsevier: Scopus)
73. Thi Nguyen Men, Kazuhiro Kikuchi, Michiko Nakai, Atsunori Fukuda, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Viet Nguyen Linh, Xuan Bui Nguyen, Takashi Nagai and Atsushi Tajima :
Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection.,
Theriogenology, Vol.80, No.9, 1033-1044, 2013.
(DOI: 10.1016/j.theriogenology.2013.08.001,   PubMed: 24041826)
74. István Egerszegi, Tamás Somfai, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Takashi Nagai, József Rátky and Kazuhiro Kikuchi :
Comparison of cytoskeletal integrity, fertilization and developmental competence of oocytes vitrified before or after in vitro maturation in a porcine model.,
Cryobiology, Vol.67, No.3, 287-292, 2013.
(DOI: 10.1016/j.cryobiol.2013.08.009,   PubMed: 23993921)
75. Hiroyuki Kaneko, Michiko Nakai, Fuminori Tanihara, Junko Noguchi and Kazuhiro Kikuchi :
Improved developmental ability of porcine oocytes grown in nude mice after fusion with cytoplasmic fragments prepared by centrifugation: a model for utilization of primordial oocytes.,
Theriogenology, Vol.80, No.8, 887-892, 2013.
(DOI: 10.1016/j.theriogenology.2013.07.014,   PubMed: 23981298)
76. Nguyen Linh Viet, Kazuhiro Kikuchi, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Quang Thanh Dang-Nguyen, Thi Nguyen Men, Nguyen Hanh Van, Tamas Somfai, Xuan Bui Nguyen, Takashi Nagai and Noboru Manabe :
Fertilization ability of porcine oocytes reconstructed from ooplasmic fragments produced and characterized after serial centrifugations.,
The Journal of Reproduction and Development, Vol.59, No.6, 549-556, 2013.
(DOI: 10.1262/jrd.2013-042,   PubMed: 23965685,   Elsevier: Scopus)
77. Hiroyuki Kaneko, Kazuhiro Kikuchi, Michiko Nakai, Tamas Somfai, Junko Noguchi, Fuminori Tanihara, Junya Ito and Naomi Kashiwazaki :
Generation of live piglets for the first time using sperm retrieved from immature testicular tissue cryopreserved and grafted into nude mice.,
PLoS ONE, Vol.8, No.7, e70989, 2013.
(DOI: 10.1371/journal.pone.0070989,   PubMed: 23923039,   Elsevier: Scopus)
78. Fuminori Tanihara, Michiko Nakai, Hiroyuki Kaneko, Junko Noguchi, Takeshige Otoi and Kazuhiro Kikuchi :
Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.,
The Journal of Reproduction and Development, Vol.59, No.4, 385-392, 2013.
(DOI: 10.1262/jrd.2013-021,   PubMed: 23666494,   Elsevier: Scopus)
79. Tamás Somfai, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Naomi Kashiwazaki, István Egerszegi, Takashi Nagai and Kazuhiro Kikuchi :
Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes.,
The Journal of Reproduction and Development, Vol.59, No.4, 378-384, 2013.
(DOI: 10.1262/jrd.2013-015,   PubMed: 23666455,   CiNii: 1390282681312531072,   Elsevier: Scopus)
80. Vien Viet Luu, Keisuke Hanatate, Fuminori Tanihara, Yoko Sato, Lanh Thi Kim Do, Masayasu Taniguchi and Takeshige Otoi :
The effect of relaxin supplementation of in vitro maturation medium on the development of cat oocytes obtained from ovaries stored at 4°C.,
Reproductive Biology, Vol.13, No.2, 122-126, 2013.
(DOI: 10.1016/j.repbio.2013.04.002,   PubMed: 23719116,   Elsevier: Scopus)
81. Zhao Namula, Yoko Sato, Risa Kodama, Kouta Morinaga, Viet Vien Luu, Masayasu Taniguchi, Michiko Nakai, Fuminori Tanihara, Kazuhiro Kikuchi, Takashi Nagai and Takeshige Otoi :
Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks.,
Animal Science Journal, Vol.84, No.8, 600-606, 2013.
(DOI: 10.1111/asj.12049,   PubMed: 23607795,   Elsevier: Scopus)
82. Hiroyuki Kaneko, Kazuhiro Kikuchi, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Michiko Noguchi, Junya Ito and Naomi Kashiwazaki :
Normal reproductive development of offspring derived by intracytoplasmic injection of porcine sperm grown in host mice.,
Theriogenology, Vol.78, No.4, 898-906, 2012.
(DOI: 10.1016/j.theriogenology.2012.04.004,   PubMed: 22626781)
83. Y Kaedei, M Naito, H Naoi, Y Sato, M Taniguchi, Fuminori Tanihara, K Kikuchi, T Nagai and Takeshige Otoi :
Effects of (-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.47, No.6, 880-886, 2012.
(DOI: 10.1111/j.1439-0531.2012.01984.x,   PubMed: 22299777,   Elsevier: Scopus)
84. Masayasu Taniguchi, Rie Arikawa, Yukine Kaedei, Fuminori Tanihara, Zhao Namula, Vien Luu Viet, Yoko Sato and Takeshige Otoi :
Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes.,
Cryo Letters, Vol.32, No.5, 410-414, 2011.
(PubMed: 22020463,   Elsevier: Scopus)
85. T Terazono, Y Kaedei, Fuminori Tanihara, Z Namula, Vl Viet, M Takagi, M Inoue, Y Sato, M Taniguchi and Takeshige Otoi :
Follicle formation in the canine ovary after autografting to a peripheral site.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.47, No.2, e16-21, 2011.
(DOI: 10.1111/j.1439-0531.2011.01880.x,   PubMed: 21883513,   Elsevier: Scopus)
86. T Terazono, M Inoue, Y Kaedei, Fuminori Tanihara, Z Namula, L V Viet, Y Taura, M Takagi, T Takuma and Takeshige Otoi :
Assessment of canine ovaries autografted to various body sites.,
Theriogenology, Vol.77, No.1, 131-138, 2011.
(DOI: 10.1016/j.theriogenology.2011.07.026,   PubMed: 21872320,   Elsevier: Scopus)
87. Masao Murakami, Juan Ya Dong, Tatsuyuki Suzuki, Masayasu Taniguchi, Yukine Kaedei, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media.,
Cryobiology, Vol.63, No.3, 170-174, 2011.
(DOI: 10.1016/j.cryobiol.2011.06.002,   PubMed: 21893054,   Elsevier: Scopus)
88. V E Abakushina, Y Morita, Y Kaedei, Fuminori Tanihara, Z Namula, L V Viet and Takeshige Otoi :
Formation of an antral follicle-like structure of bovine cumulus-oocyte complexes embedded individually or in groups in collagen gels.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.46, No.3, 423-427, 2010.
(DOI: 10.1111/j.1439-0531.2010.01684.x,   PubMed: 20723136,   Elsevier: Scopus)
89. Yavari Morteza, Naoi Hideaki, Kaedei Yukine, Fuminori Tanihara, Namula Zhao, Viet Luu Vien and Takeshige Otoi :
Effects of epigallocatechin- 3-gallate on the developmental competence of parthenogenetic embryos in the pig,
Italian Journal of Animal Science, Vol.9, No.4, 386-389, 2010.
(DOI: 10.4081/ijas.2010.e73)
90. Kaedei Yukine, Fujiwara Akira, Ito Aya, Fuminori Tanihara, Morita Yasuhiro, Hanatate Keisuke, Viet Luu Vien, Namula Zhao and Takeshige Otoi :
Effect of Roscovitine Pretreatment on the Meiotic Maturation of Bovine Oocytes and their Subsequent Development after Somatic Cell Nuclear Transfer,
Journal of Animal and Veterinary Advances, Vol.9, No.22, 2848-2853, 2010.
(DOI: 10.3923/javaa.2010.2848.2853,   Elsevier: Scopus)
91. Y Kaedei, A Fujiwara, Fuminori Tanihara, Z Namula, VL Viet and Takeshige Otoi :
In vitro development of cat interspecies nuclear transfer using pig's and cow's cytoplasm,
Bulletin of the Veterinary Institute in Puławy, Vol.54, No.3, 405-408, 2010.
(Elsevier: Scopus)
92. A Fujii, Y Kaedei, Fuminori Tanihara, A Ito, K Hanatate, K Kikuchi, T Nagai and Takeshige Otoi :
In vitro maturation and development of porcine oocytes cultured in a straw or dish using a portable incubator with a CO2 chamber.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.45, No.4, 619-624, 2009.
(DOI: 10.1111/j.1439-0531.2008.01316.x,   PubMed: 19144033,   Elsevier: Scopus)
93. Agung Budiyanto, Barati Farid, Kaedei Yukine, Fuminori Tanihara and Takeshige Otoi :
Meiotic Competence and DNA Damage of Porcine Oocytes from Ovaries Exposed to an Elevated Temperature,
Journal of Animal and Veterinary Advances, Vol.7, No.10, 1179-1183, 2008.
(Elsevier: Scopus)
94. M. Yavari, A. Fujii, R. Shimizu, A. Ito, Y. Kaedei, Y. Morita, Fuminori Tanihara and Takeshige Otoi :
Meiotic competence of porcine oocytes after percoll sedimentation treatment for oocyte selection.,
Journal of Animal Veterinary advances, Vol.6, No.11, 1333-1336, 2007.

Review, Commentary:

1. Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
ゲノム編集技術による遺伝子改変家畜の作出,
Journal of Mammalian Ova Research, Vol.39, 17-26, Jan. 2022.
(CiNii: 1050858518835823232)

Proceeding of International Conference:

1. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
2. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Hirano Takayuki, Le Anh Quynh and Takeshige Otoi :
Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
3. Le Anh Quynh, Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Hirano Takayuki and Takeshige Otoi :
Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
4. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
5. Akio Kuroda, Misuzu Yamada, Yukari Tominaga, Reiko Suzuki, Motoyuki Tamaki, Yuko Akehi, Yuichi Takashi, Daisuke Otsuka, Eisuke Shimokita, Fuminori Tanihara, Kiyoe Kurahashi, Sumiko Yoshida, Itsuro Endo, Ken-ichi Aihara, Masahiro Abe, Kevin Ferreri and Munehide Matsuhisa :
Detection of pancreatic beta cell DNA in the circulation using the amplification refractory mustation system PCR,
American Diabetes Association 78th Scientific Sessions, Orlando, Jun. 2018.
6. Katsutoshi Nishio, Fuminori Tanihara, TV Nguyen, Toshiki Kunihara and Takeshige Otoi :
Effects of voltage strength on development and quality of electroporated porcine embryos.,
Transgenic Research, Vol.26, No.1, 29, Utah, USA, Oct. 2017.
7. Fuminori Tanihara, LTK Do, TV Nguyen, Toshiki Kunihara, Katsutoshi Nishio, Tatsuya Takemoto and Takeshige Otoi :
Generation of TP53-modified pigs by GEEP method: CRISPR/Cas9-mediated gene modification introduced into porcine zygotes by electroporation.,
Transgenic Research, Vol.26, No.1, 38, Utah, USA, Oct. 2017.
8. Fuminori Tanihara and Takeshige Otoi :
A simple-step generation of genetically modified pigs by genome editing by electroporation of Cas9 protein (GEEP) method.,
Forth World Congress of Reproductive Bioligy (WCRB2017), Okinawa, Sep. 2017.
9. R Kuriwaki, Y Sato, S Hagino, M Shimazaki, R Sambuu, LTK Do, Fuminori Tanihara, M Takagi, M Taniguchi and Takeshige Otoi :
Evaluation of the Leydig cell function on crossbreeding yak showing infertility.,
Forth World Congress of Reproductive Bioligy (WCRB2017), Okinawa, Sep. 2017.

Proceeding of Domestic Conference:

1. Fuminori Tanihara, Maki Hirata, 野口 光央, 魚崎 英毅, 本多 新, Takeshige Otoi and 花園 豊 :
ゲノム編集による生物医学研究・外科トレーニング用ブタの作出,
日本ゲノム編集学会 第8回大会, Jun. 2023.
2. Q Lin, Quynh Anh Le, Manita Wittayarat, Zhao Namula, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Kim Lanh Thi Do and Takeshige Otoi :
Triple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.,
第7回ゲノム編集学会, Jun. 2022.
3. Lin Qingyi, Chommanart Thongkittidilok, Maki Hirata, QUYNH ANH LE, K. Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Lipofection-mediated introduction of CRISPR/Cas9 system into porcine zygotes.,
第114回日本繁殖生物学会, Sep. 2021.
4. TAKEBAYASHI Kohki, Chommanart Thongkittidilok, 林 青怡, LE Anh Quynh, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
ノックアウトブタ由来精子を用いた体外受精胚におけるゲノム編集効率の検討,
第114回日本繁殖生物学会, Sep. 2021.
5. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製,
第162回日本獣医学会学術集会, Sep. 2019.
6. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Namula Zhao and Takeshige Otoi :
ブタ体外受精卵におけるCrispr/Cas9システムを使用したゲノム編集の効率,
日本ゲノム編集学会 第3回大会, Jun. 2018.
7. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano and Takeshige Otoi :
ブタ内在性レトロウイルス遺伝子を標的としたゲノム編集が胚発育能に及ぼす影響,
日本ゲノム編集学会第3回大会, Jun. 2018.
8. Fuminori Tanihara :
エレクトロポレーションによるブタ胚へのCRISPR/Cas9 システムの導入とゲノム編集ブタの作製,
第40回日本分子生物学会年会, Dec. 2017.

Et cetera, Workshop:

1. Fuminori Tanihara, Maki Hirata, Takeshige Otoi, 澤本 修 and 菊地 健志 :
GEEP法によるGGTA1遺伝子およびCMAH遺伝子の同時改変ブタの作出,
第24回日本異種移植研究会, Feb. 2021.
2. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
CRISPR/Cas9システムによるブタ体外受精卵のINS遺伝子への点変異導入,
第7回日本先進医工学ブタ研究会, Oct. 2019.
3. Takeshige Otoi, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Fuminori Tanihara :
GEEP法を用いた遺伝子改変ブタの作製と遺伝子改変効率,
第7回日本先進医工学ブタ研究会, Oct. 2019.
4. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
ブタ体外受精卵におけるCRISPR/Cas9システムを用いた複数遺伝子の同時改変,
第7回日本先進医工学ブタ研究会, Oct. 2019.
5. Le Anh Quynh, Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano and Takeshige Otoi :
Concentration of CRISPR/Cas9 components effects on genetic mosaicism of cytoplasmic microinjected porcine embryos,
第6回日本先進医工学ブタ研究会, Oct. 2018.
6. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Le Anh Quynh, 新居 雅宏 and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid,
第6回日本先進医工学ブタ研究会, Oct. 2018.
7. Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, 新居 雅宏 and Takeshige Otoi :
ブタにおける受精前後でのゲノム編集が胚盤胞の変異導入効率に及ぼす影響,
第6回日本先進医工学ブタ研究会, Oct. 2018.
8. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Takayuki Hirano, Tatsuya Takemoto, 中井 美智子, 淵本 大一郎 and Takeshige Otoi :
ゲノム編集によるTP53遺伝子改変ブタの作製と表現型の解析,
第6回日本先進医工学ブタ研究会, Oct. 2018.
9. Fuminori Tanihara, Tatsuya Takemoto, Maki Hirata, N Nguyen Thi, Toshiki Kunihara, R Nishinakamura and Takeshige Otoi :
Modification of SALL1 gene via CRISPR/Cas9-mediated gene editing introduced into porcine zygotes by electroporation,
KEY Forum: The 3rd International symposium on Stem Cell Traits and Developmental Systems, Jan. 2018.
10. Fuminori Tanihara and Takeshige Otoi :
ゲノム編集技術を用いたブタでの応用例,
第3回日本生物工学会西日本支部講演会「ゲノム編集—多様な生物種への応用研究」, Dec. 2016.
11. Fuminori Tanihara, Tatsuya Takemoto, Michiko Nakai, Eri Kitagawa, Do Thi Kim Lanh, Akira Onishi, Shunichi Suzuki, Syoichiro Senbon, Daiichiro Fuchimoto and Takeshige Otoi :
新規ゲノム編集技術を用いたPDX-1遺伝子改変ブタの作製,
第4回 日本先進医工学ブタ研究会 要旨集, 21, Oct. 2016.

Patent:

1. Tatsuya Takemoto, 橋本 昌和, Takeshige Otoi and Fuminori Tanihara : 2016-074645 (Apr. 2016), 2016-074645 (Apr. 2016).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

  • Development of animal model for kidney-specific spontaneous tumours (Project/Area Number: 22K19896 )
  • 慢性腎臓病に起因する健康寿命減衰をリン(CPP)の除去により防ぎ止める橋渡し研究 (Project/Area Number: 23K23805 )
  • Generation of African Swine Fever virus-resistant pigs by applying the concept of gene editing technology (Project/Area Number: 21KK0124 )
  • Generation of a pig model for the diabetic nephropathy using gene editing technology (Project/Area Number: 19K16014 )
  • Production of immunodeficient micro-mini pig using genome editing technology. (Project/Area Number: 18K12062 )
  • The role of p53 during airway and lung injury, repair and regeneration using TP53-mutant porcine (Project/Area Number: 18K08785 )
  • Production of pigs for safe xenotransplantation by controlling infectious porcine endogenous retrovirus (PERV) (Project/Area Number: 17K19325 )
  • Production of genetically modified pigs by combined techniques of chromosome engineering and genome editing (Project/Area Number: 17H03938 )
  • Search by Researcher Number (90754680)