Tokushima University / Educator and Researcher Directory --- Akazawa, Yuki

Field of Study

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1.	Anrizandy Narwidina, Aya Miyazaki, Kokoro Iwata, Rika Kurogoushi, Asuna Sugimoto, Yasusei Kudo, Keita Kawarabayashi, Yoshihito Yamakawa, Yuki Akazawa, Takamasa Kitamura, Hiroshi Nakagawa, Kimiko Ueda Yamaguchi, Tomokazu Hasegawa, Keigo Yoshizaki, Satoshi Fukumoto, Akihito Yamamoto, Naozumi Ishimaru, Tomonori Iwasaki and Tsutomu Iwamoto : Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression., Biochemical and Biophysical Research Communications, Vol.650, 47-54, 2023. (Summary) Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation. (Keyword) Homeodomain Proteins / Transcription Factors / Odontoblasts / Genes, Homeobox / cell differentiation / cell proliferation and differentiation (Tokushima University Institutional Repository) ● Metadata: 118052 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2023.02.004 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36773339 ● Search Scopus @ Elsevier (PMID): 36773339 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2023.02.004 (Tokushima University Institutional Repository: 118052, DOI: 10.1016/j.bbrc.2023.02.004, PubMed: 36773339)
2.	Rika Kurogoushi, Kokoro Iwata, Hiroshi Nakagawa, Tomokazu Hasegawa, Kimiko Ueda Yamaguchi, Takamasa Kitamura, Yuki Akazawa, Asuna Sugimoto, Keita Kawarabayashi, Aya Miyazaki, Rie Niji, 藤島史帆, Tomonori Iwasaki and Tsutomu Iwamoto : 当科における5年間の障害児・有病児の初診時実態調査, The Japanese Journal of Pediatric Dentistry, Vol.60, No.1, 20-27, 2022. (Summary) 障害児や有病児への理解の深まりから社会環境や福祉制度の整備が進みつつあるが，障害や全身疾患をもつ児が地域において疾病予防も含めた歯科医療を受けられる環境がいまだ十分にあるとは言えず，実情に応じた環境整備が求められる．そこで，今回，平成25年度から平成29年度に当科を受診した児のうち障害児・有病児の5年間の初診時実態調査を行い，過去に当科で行った調査と比較し，近年の変化を検討した．初診患者1,301名のうち，障害児・有病児の割合は49 (Keyword) う蝕先天奇形口蓋裂口唇裂障害者歯科医療発達障害染色体異常小児歯科医療慢性疾患患者歯科医療病院歯科診療部門紹介と相談年齢因子徳島県実態調査地域差ヒト小児(6∼12) 男女歯学 (Link to Publication Site) ● Publication site (DOI): 10.11411/jspd.60.1_20 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390295259235197952 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.60.1_20 (DOI: 10.11411/jspd.60.1_20, CiNii: 1390295259235197952)
3.	Rika Kurogoushi, Tomokazu Hasegawa, Yuki Akazawa, Kokoro Iwata, Asuna Sugimoto, Kimiko Ueda Yamaguchi, Aya Miyazaki, Anrizandy Narwidina, Keita Kawarabayashi, Takamasa Kitamura, Hiroshi Nakagawa, Tomonori Iwasaki and Tsutomu Iwamoto : Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells, Experimental and Therapeutic Medicine, Vol.22, No.6, 1356, 2021. (Summary) The regulation of the mesenchymal stem cell (MSC) programming mechanism promises great success in regenerative medicine. Tissue regeneration has been associated not only with the differentiation of MSCs, but also with the microenvironment of the stem cell niche that involves various cytokines and immune cells in the tissue regeneration site. In the present study, fibroblast growth factor 2 (FGF2), the principal growth factor for tooth development, dental pulp homeostasis and dentin repair, was reported to affect the expression of cytokines in human dental pulp-derived MSCs. FGF2 significantly inhibited the expression of chemokine C-C motif ligand 11 (CCL11) in a time- and dose-dependent manner in the SDP11 human dental pulp-derived MSC line. This inhibition was diminished following treatment with the AZD4547 FGF receptor (FGFR) inhibitor, indicating that FGF2 negatively regulated the expression of CCL11 in SDP11 cells. Furthermore, FGF2 activated the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNK) in SDP11 cells. The mechanism of the FGFR-downstream signaling pathway was then studied using the SB203580, U0126 and SP600125 inhibitors for p38 MAPK, ERK1/2, and JNK, respectively. Interestingly, only treatment with SP600125 blocked the FGF2-mediated suppression of CCL11. The present results suggested that FGF2 regulated the expression of cytokines and suppressed the expression of CCL11 via the JNK signaling pathway in human dental pulp-derived MSCs. The present findings could provide important insights into the association of FGF2 and CCL11 in dental tissue regeneration therapy. (Tokushima University Institutional Repository) ● Metadata: 117039 (Link to Publication Site) ● Publication site (DOI): 10.3892/etm.2021.10791 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34659502 ● Search Scopus @ Elsevier (PMID): 34659502 ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2021.10791 (Tokushima University Institutional Repository: 117039, DOI: 10.3892/etm.2021.10791, PubMed: 34659502)
4.	岩田こころ, Rika Kurogoushi, Asuna Sugimoto, 藤島史帆, Yuki Akazawa, Keita Kawarabayashi, Aya Miyazaki, Takamasa Kitamura, Rie Niji, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa, Tomokazu Hasegawa and Tsutomu Iwamoto : Survey of the Actual State of New Patients at the Pediatric Dentistry Department of Tokushima University Hospital between 1989 and 2018, The Japanese Journal of Pediatric Dentistry, Vol.59, No.1, 8-13, 2021. (Summary) <p>The Heisei Era ended at the end of April 2019 and the Reiwa Era began.</p><p>For about 30 years in the Heisei era after the Showa era, the environment surrounding children changed rapidly, including the economic downturn, the acceleration of late marriage and declining birthrate, and progress in information and communication technology. As a result, individual households have diversified and a large polarization has become apparent. As pediatric dentists, we need to deal with and support children with all kinds of problems. Therefore, to clarify the transition of the first-visit patients in our clinic, we categorized the 30 years from 1989 to 2018 by five-year groups: early (1989-1993), middle (1997-2001) and late (2013-2018) stages, with a total of 5,892 patients, and then investigated and analyzed their properties. As a result, the number of new patients decreased from 2,477 in the early stage to 1,301 in the late stage. The male-female ratio was approximately 1:1 in each period. As for age, school age was the highest at about 40%, and there was no significant change in the age composition in each period. The most common chief complaint during each period was tooth decay, but it decreased from 44% in the early stage to 27% in the late stage. The proportion of children who brought a letter of referral increased from 23% in the early stage to 83% in the late stage, and the proportion of children with disabilities and illness increased from 13.8% in the early stage to 33% in the late stage. Compared to the early days, the chief complaints have diversified, and it has become clear that the functional differentiation of general dental clinics and university hospitals is progressing. Furthermore, it was suggested that the importance of medical and dental cooperation will increase in the future.</p> (Keyword) 実態調査 / 初診患者 / 平成 / 小児歯科 / Survey of actual state / New patients / Heisei / Pediatric dentistry (Link to Publication Site) ● Publication site (DOI): 10.11411/jspd.59.1_8 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390854139452228096 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.59.1_8 (DOI: 10.11411/jspd.59.1_8, CiNii: 1390854139452228096)
5.	Aya Miyazaki, Asuna Sugimoto, Keigo Yoshizaki, Keita Kawarabayashi, Kokoro Iwata, Rika Kurogohshi, Takamasa Kitamura, Kunihiro Otsuka, Tomokazu Hasegawa, Yuki Akazawa, Satoshi Fukumoto, Naozumi Ishimaru and Tsutomu Iwamoto : Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1, Scientific Reports, Vol.9, No.1, 14762, 2019. (Summary) Signal transmission from the mechanical forces to the various intracellular activities is a fundamental process during tissue development. Despite their critical role, the mechanism of mechanical forces in the biological process is poorly understood. In this study, we demonstrated that in the response to hydrostatic pressure (HP), the piezo type mechanosensitive ion channel component 1 (PIEZO1) is a primary mechanosensing receptor for odontoblast differentiation through coordination of the WNT expression and ciliogenesis. In stem cells from human exfoliated deciduous teeth (SHED), HP significantly promoted calcium deposition as well as the expression of odontogenic marker genes, PANX3 and DSPP, and WNT related-genes including WNT5b and WNT16, whereas HP inhibited cell proliferation and enhanced primary cilia expression. WNT signaling inhibitor XAV939 and primary cilia inhibitor chloral hydrate blocked the HP-induced calcium deposition. The PIEZO1 activator Yoda1 inhibited cell proliferation but induced ciliogenesis and WNT16 expression. Interestingly, HP and Yoda1 promoted nuclear translocation of RUNX2, whereas siRNA-mediated silencing of PIEZO1 decreased HP-induced nuclear translocation of RUNX2. Taken together, these results suggest that PIEZO1 functions as a mechanotransducer that connects HP signal to the intracellular signalings during odontoblast differentiation. (Tokushima University Institutional Repository) ● Metadata: 114667 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-019-51381-9 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31611621 ● Search Scopus @ Elsevier (PMID): 31611621 ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-019-51381-9 (Tokushima University Institutional Repository: 114667, DOI: 10.1038/s41598-019-51381-9, PubMed: 31611621)
6.	Kimiko Ueda Yamaguchi, Yuki Akazawa, Kawarabayashi Keita, Asuna Sugimoto, Hiroshi Nakagawa, miyazaki aya, Weih Falk, Kurogoushi Rika, Iwata Kokoro, Takamasa Kitamura, Yamada Aya, Tomokazu Hasegawa, Fukumoto Satoshi and Tsutomu Iwamoto : Combination of ions promotes cell migration via extracellular signalregulated kinase 1/2 signaling pathway in human gingival fibroblasts., Molecular Medicine Reports, Vol.19, No.6, 5039-5045, 2019. (Summary) Wound healing is a dynamic process that involves highly coordinated cellular events, including proliferation and migration. Oral gingival fibroblasts serve a central role in maintaining oral mucosa homeostasis, and their functions include the coordination of physiological tissue repair. Recently, surface pre-reacted glass-ionomer (S-PRG) fillers have been widely applied in the field of dental materials for the prevention of dental caries, due to an excellent ability to release fluoride (F). In addition to F, S-PRG fillers are known to release several types of ions, including aluminum (Al), boron (B), sodium (Na), silicon (Si) and strontium (Sr). However, the influence of these ions on gingival fibroblasts remains unknown. The aim of the present study was to examine the effect of various concentrations of an S-PRG filler eluate on the growth and migration of gingival fibroblasts. The human gingival fibroblast cell line HGF-1 was treated with various dilutions of an eluent solution of S-PRG, which contained 32.0 ppm Al, 1,488.6 ppm B, 505.0 ppm Na, 12.9 ppm Si, 156.5 ppm Sr and 136.5 ppm F. Treatment with eluate at a dilution of 1:10,000 was observed to significantly promote the migration of HGF-1 cells. In addition, the current study evaluated the mechanism underlying the mediated cell migration by the S-PRG solution and revealed that it activated the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), but not of p38. Furthermore, treatment with a MEK inhibitor blocked the cell migration induced by the solution. Taken together, these results suggest that S-PRG fillers can stimulate HGF-1 cell migration via the ERK1/2 signaling pathway, indicating that a dental material containing this type of filler is useful for oral mucosa homeostasis and wound healing. (Tokushima University Institutional Repository) ● Metadata: 113289 (Link to Publication Site) ● Publication site (DOI): 10.3892/mmr.2019.10141 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31059063 ● Summary page in Scopus @ Elsevier: 2-s2.0-85066160258 (Tokushima University Institutional Repository: 113289, DOI: 10.3892/mmr.2019.10141, PubMed: 31059063, Elsevier: Scopus)
7.	Keita Kawarabayashi, Asuna Sugimoto, Yuki Akazawa, Kimiko Ueda Yamaguchi, Takamasa Kitamura, Aya Miyazaki and Tsutomu Iwamoto : Association Between Dental Caries and Lifestyle in Children, The Japanese Journal of Pediatric Dentistry, Vol.56, No.1, 26-32, 2018. (Summary) <p>The prevalence of dental caries is low in younger children, while rapid progression occurs in adolescence. The purpose of this study was to clarify the association of prevalence of dental caries in children and daily lifestyle habits. A total of 100 children (57 boys, 43 girls, average age 4 years 6 months) who visited our department were enrolled and divided into the caries and non-caries group. Each parent or guardian was asked regarding the daily lifestyle of the child, including nutrition and oral health. We found that "maternal employment", "bedtime", and "frequent snacks" were correlated with dental caries prevalence. There was no significant differences in regard to "living with grandparents","presence of brothers and/or sisters", "wake-up time", and "knowledge of dental health". Furthermore, we found that all children with caries ate excessively during meals, excluding snacks. Dental health promotion contributes to specifically target dental caries, while establishment of healthy lifestyle habits in children, particularly healthy habits for eating, is important.</p><p></p> (Keyword) 齲蝕罹患，生活習慣，自律支援 (Link to Publication Site) ● Publication site (DOI): 10.11411/jspd.56.1_26 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390845713054285568 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.56.1_26 (DOI: 10.11411/jspd.56.1_26, CiNii: 1390845713054285568)
8.	Asuna Sugimoto, Yuki Akazawa, Keita Kawarabayashi, Aya Miyazaki, Kimiko Ueda Yamaguchi, Takamasa Kitamura and Tsutomu Iwamoto : A study on the effect of Oral Myofunctional Therapy (MFT) in children, The Japanese Journal of Pediatric Dentistry, Vol.56, No.1, 1-11, 2018. (Summary) <p>Oral myofunctional therapy (MFT) is a specialized training technique taught to patients for selftreatment of a variety of oral muscle functional disorders and habit patterns that disrupt normal oral and dental development. Although various training methods have been proposed, there are few reports on the effects of training in individual subjects. The purpose of this study was to clarify the effects of various MFT methods in 20 children (15 boys, 5 girls : average age 7 years 7 months) who underwent training at our hospital. The results revealed that the percentage of children with poor lip seal improved from 55% to 33%,while tongue thrust during swallowing and articulating improved from 100% to 50% and 95% to 60% respectively. We also found that "Water Slurp Swallow" training significantly contributed to improved tongue thrust during swallowing. However, even though the subjects were able to master the training methods, they failed to obtain correct oral functions, thus new training methods as well as the timing for starting training must be considered. Oral function is regarded as important throughout life, with childhood a crucial stage for establishing a healthy foundation. Our results also showed that 20% of the child subjects had oral functional difficulties without morphological problems, indicating that both morphological and functional problems should be evaluated. MFT is an effective method for support of oral function and development.</p><p></p> (Keyword) 口腔筋機能療法 / MFT / infant (Link to Publication Site) ● Publication site (DOI): 10.11411/jspd.56.1_1 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390564238077774720 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.56.1_1 (DOI: 10.11411/jspd.56.1_1, CiNii: 1390564238077774720)
9.	Asuna Sugimoto, Aya Miyazaki, Keita Kawarabayashi, Masayuki Shono, Yuki Akazawa, Tomokazu Hasegawa, Kimiko Ueda Yamaguchi, Takamasa Kitamura, Yoshizaki keigo, Fukumoto Satoshi and Tsutomu Iwamoto : Piezo type mechanosensitive ion channel component 1 functions as a regulator of the cell fate determination of mesenchymal stem cells, Scientific Reports, Vol.7, No.1, 17696, 2017. (Summary) The extracellular environment regulates the dynamic behaviors of cells. However, the effects of hydrostatic pressure (HP) on cell fate determination of mesenchymal stem cells (MSCs) are not clearly understood. Here, we established a cell culture chamber to control HP. Using this system, we found that the promotion of osteogenic differentiation by HP is depend on bone morphogenetic protein 2 (BMP2) expression regulated by Piezo type mechanosensitive ion channel component 1 (PIEZO1) in MSCs. The PIEZO1 was expressed and induced after HP loading in primary MSCs and MSC lines, UE7T-13 and SDP11. HP and Yoda1, an activator of PIEZO1, promoted BMP2 expression and osteoblast differentiation, whereas inhibits adipocyte differentiation. Conversely, PIEZO1 inhibition reduced osteoblast differentiation and BMP2 expression. Furthermore, Blocking of BMP2 function by noggin inhibits HP induced osteogenic maker genes expression. In addition, in an in vivo model of medaka with HP loading, HP promoted caudal fin ray development whereas inhibition of piezo1 using GsMTx4 suppressed its development. Thus, our results suggested that PIEZO1 is responsible for HP and could functions as a factor for cell fate determination of MSCs by regulating BMP2 expression. (Tokushima University Institutional Repository) ● Metadata: 112396 (Link to Publication Site) ● Publication site (DOI): 10.1038/s41598-017-18089-0 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29255201 ● Summary page in Scopus @ Elsevier: 2-s2.0-85038567061 (Tokushima University Institutional Repository: 112396, DOI: 10.1038/s41598-017-18089-0, PubMed: 29255201, Elsevier: Scopus)
10.	Yuki Akazawa, Tomokazu Hasegawa, Yoshitaka Yoshimura, Naoyuki Chosa, Takeyoshi Asakawa, Kimiko Ueda Yamaguchi, Asuna Sugimoto, Takamasa Kitamura, Hiroshi Nakagawa, Akira Ishisaki and Tsutomu Iwamoto : Recruitment of mesenchymal stem cells by stromal cell-derived factor 1 in pulp cells from deciduous teeth, International Journal of Molecular Medicine, Vol.36, 442-448, 2015. (Summary) Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. Therefore, the aim of the present in vitro study was to investigate whether stromal cell-derived factor 1 (SDF1)-C-X-C chemokine receptor type 4 (CXCR4) signaling is involved in tissue repair in the DP of deciduous teeth. A single-cell clone from DPCs (SDP11) and UE7T-13 cells were used as pulp cells and MSCs, respectively. The MG-63 and HuO9 cells, two osteosarcoma cell lines, were used as positive control cells. Reverse transcription polymerase chain reaction (RT-PCR) revealed that all cell lines (SDP11, UE7T-13 MG-63 and HuO9) were positive for both SDF1 and CXCR4 mRNA expression. Moreover, immunocytochemical analysis indicated that SDF1 and CXCR4 proteins were expressed in the SDP11 and UE7T-13 cells. SDF1 was also detected in the cell lysates (CLs) and conditioned medium (CM) collected from the SDP11 and UE7T-13 cells, and AMD3100, a specific antagonist of CXCR4, inhibited the migration of the UE7T-13 cells; this migration was induced by treatment with CM, which was collected from the SDP11 cells. In addition, real-time PCR showed that the expression of SDF1 in the SDP11 cells was inhibited by treatment with 20 ng/ml fibroblast growth factor (FGF)-2, and exposure to AZD4547, an inhibitor of the FGF receptor, blocked this inhibition. Collectively, these data suggest that SDF1 produced by DP plays an important role in homeostasis, repair and regeneration via the recruitment of MSCs. (Link to Publication Site) ● Publication site (DOI): 10.3892/ijmm.2015.2247 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26082290 ● Summary page in Scopus @ Elsevier: 2-s2.0-84934271560 (DOI: 10.3892/ijmm.2015.2247, PubMed: 26082290, Elsevier: Scopus)
11.	Tomokazu Hasegawa, Yuki Akazawa, Takamasa Kitamura, Asuna Sugimoto, Kimiko Ueda Yamaguchi and Tsutomu Iwamoto : Dental findings and management in a child with hypomelanosis of Ito, Pediatric Dental Journal, Vol.24, No.3, 173-177, 2014. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.pdj.2014.08.002 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84938485288 (DOI: 10.1016/j.pdj.2014.08.002, Elsevier: Scopus)
12.	Yuki Akazawa, Takamasa Kitamura, Yuri Fujihara, Yoshitaka Yoshimura, Masato Mitome and Tomokazu Hasegawa : Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus., International Journal of Molecular Medicine, Vol.31, No.2, 307-314, 2012. (Summary) In this study, we examined the effect of forced mastication on neurogenesis in the hippocampal dentate gyrus (DG) of adult mice. Six-week-old mice were subjected to either a hard or normal diet for 13 weeks. They received a daily injection of bromodeoxyuridine (BrdU) for 12 consecutive days beginning at 14 weeks of age. The number of BrdU-positive cells in the DG was counted 1 day after and 5 weeks after the final BrdU injection. The number of BrdU-positive cells 1 day after injection did not differ between the 2 diet groups. However, the number of BrdU-positive cells in the group fed the hard diet was significantly increased 5 weeks after BrdU injection compared to the group fed the normal diet. The results of the Morris water maze test showed that mice fed a hard diet required significantly less time to reach the platform than the control mice when tested at 10 days. Moreover, mice in the group fed the hard diet spent significantly more time in the former platform area than the group fed the normal diet, indicating that hard diet feeding improved spatial memory compared to normal diet feeding. Real-time PCR analysis showed that the expression of glutamate receptor 1 mRNA was significantly increased in the group fed the hard diet compared with the group fed the normal diet. These results suggest that mastication increases the survival of adult neural stem cells in the hippocampal DG. (Keyword) Adult Stem Cells / Animals / Cell Proliferation / Cell Survival / Dentate Gyrus / Diet / Eating / Gene Expression Regulation, Developmental / Intercellular Signaling Peptides and Proteins / Mastication / Mice / Mice, Inbred C57BL / Nerve Growth Factors / Neural Stem Cells / Neurogenesis / RNA, Messenger (Link to Publication Site) ● Publication site (DOI): 10.3892/ijmm.2012.1217 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23254518 ● Summary page in Scopus @ Elsevier: 2-s2.0-84873182824 (DOI: 10.3892/ijmm.2012.1217, PubMed: 23254518, Elsevier: Scopus)
13.	Yuki Akazawa : 咀嚼が海馬神経幹細胞に与える影響について, 四国歯学会誌, Vol.25, No.1, 1-17, 2012.
14.	Keiko Miyoshi, Yuki Akazawa, Taigo Horiguchi and Takafumi Noma : Localization of adenylate kinase 4 in mouse tissues., Acta Histochemica et Cytochemica, Vol.42, No.2, 55-64, 2009. (Tokushima University Institutional Repository) ● Metadata: 114185 (Link to Publication Site) ● Publication site (DOI): 10.1267/ahc.08012 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19492028 ● Summary page in Scopus @ Elsevier: 2-s2.0-66149122562 (Tokushima University Institutional Repository: 114185, DOI: 10.1267/ahc.08012, PubMed: 19492028, Elsevier: Scopus)

Review, Commentary:

1.	Kazumi Takaishi, Shigeki Fujiwara Joseph Luke, Satoru Eguchi, Ohtuka Ryo, Yuki Akazawa, Takamasa Kitamura, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa, Kohichi Kani, Keiko Aota, Yukihiro Momota, Yoshiko Yamamura, Keiko Kudoh, Go Ohe, Tetsuya Tamatani, Youji Miyamoto, Masayuki Azuma, Tsutomu Iwamoto and Hiroshi Kitahata : Dental Treatment under General Anesthesia in Tokushima University Hospital - Safety Management of Disabled Patients with Special Needs during Dental Treatment-, Journal of Oral Health and Biosciences, Vol.31, No.1, 62-67, Jul. 2018. (Summary) We report about general anesthesia for disabled patients with special needs or young children during dental treatment in Tokushima University Hospital. We have administered ambulatory anesthesia or general anesthesia with short-term hospitalization for the patients. In this report, we showed anesthesia as a behavior management technique and the system for the patients who schedule ambulatory anesthesia in our hospital. We indicated the present situation of dental treatment under general anesthesia and supposed the prospects of dental care for the patients with special needs. (Keyword) 全身麻酔 / 障害者 / 日帰り全身麻酔 / 歯科治療 (Tokushima University Institutional Repository) ● Metadata: 111940 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.31.1_62 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390845712973457920 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.31.1_62 (Tokushima University Institutional Repository: 111940, DOI: 10.20738/johb.31.1_62, CiNii: 1390845712973457920)
2.	Kimiko Ueda Yamaguchi, Keita Kawarabayashi, Aya Miyazaki, Rika Kurogohshi, Kokoro Iwata, Asuna Sugimoto, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : Prevention of Sports-related Dental Injuries in Children, Journal of Oral Health and Biosciences, Vol.31, No.1, 68-72, Jul. 2018. (Summary) A sports-related dental injury is defined as injuries to the oral and maxillofacial regions associated with sports activities, and involves tooth fracture and luxation, facial bone and temporomandibular fractures, and soft tissue injury. Participants in sports activities are always at risk for traumatic injury, with the oral and maxillofacial region often affected. Dental injuries also have a high rate of occurrence among sports-related injuries received during school physical education classes and club activities. Unfortunately, nearly all such dental injuries are irreversible, and the loss of teeth or their supporting tissues has a significant impact on the quality of life of affected individuals. Thus, for prevention of sports-related dental injuries, it is important for dental professionals to disseminate correct knowledge regarding oral health, as well as provide information to reduce and treat risk factors such as dental caries, periodontal disease, and occlusal problems. In particular, use of mouthguard is one of the most effective ways to prevent sports-related dental injuries that occur in sports and physical activity participants. Recently, along with increased health consciousness, the number of individuals who participate in sports and fitness activities is also increasing. Outside of the bounds of conventional dental clinical treatment, dentists are encouraged to actively be involved in local and regional organizations related to sports, recreation, and physical activity opportunities, in order to contribute to promotion of safety and health, including injury prevention. In this review, we discuss various findings to prevention of sports-related dental injuries in children. (Keyword) Sports injury / Mouthguard / Children (Tokushima University Institutional Repository) ● Metadata: 111941 (Link to Publication Site) ● Publication site (DOI): 10.20738/johb.31.1_68 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390845712973017344 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.31.1_68 (Tokushima University Institutional Repository: 111941, DOI: 10.20738/johb.31.1_68, CiNii: 1390845712973017344)
3.	Tsutomu Iwamoto, Asuna Sugimoto, Takamasa Kitamura, Yuki Akazawa and Tomokazu Hasegawa : The role of extracellular ATP-mediated purinergic signaling in bone, cartilage, and tooth tissue, Journal of Oral Biosciences, Vol.56, No.4, 131-135, Jul. 2014. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.job.2014.07.003 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-84927910794 (DOI: 10.1016/j.job.2014.07.003, Elsevier: Scopus)

Proceeding of International Conference:

1.	Yuki Akazawa, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa and Tomonori Iwasaki : A case of using a mouthpiece for bites in perioperative, Oral Care, Apr. 2022.
2.	Kimiko Ueda Yamaguchi, Asuna Sugimoto, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : Multi-ion solution promotes cell migration through ERK signaling pathway and CXCR4 expression in human gingival of fibroblasts, 10th Biennial Conferrence of the Pediatric Dentistry Association of Asia, Vol.54, No.2, 346, Tokyo, May 2016.
3.	Takafumi Noma, Keiko Miyoshi, Yuki Akazawa and Taigo Horiguchi : Tissue-distribution and possible functional roles of AK4, Mitochondrial Medicine Meeting, San Diego, Jun. 2007. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.mito.2007.08.016 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.mito.2007.08.016 (DOI: 10.1016/j.mito.2007.08.016)

Proceeding of Domestic Conference:

1.	Yuki Akazawa, Tomokazu Hasegawa and Tomonori Iwasaki : 多数の永久歯に著しい短根を認めた1例, The Japanese Journal of Pediatric Dentistry, Vol.62, 74, 2024.
2.	Hiroshi Nakagawa, Kimiko Ueda Yamaguchi, Takamasa Kitamura, Yuki Akazawa, 山川允仁, 前尾慶, 幸平若奈, 鈴木結加里, 兼松めぐみ, 石川英子, 岡本悦子, 森重代, 横井久美子, 森本みどり and Tomonori Iwasaki : Down症候群患者における歯周炎評価指標 (PISA)と歯周病原菌叢の関連, 第40回日本障害者歯科学会総会および学術大会, Nov. 2023.
3.	Kimiko Ueda Yamaguchi, Yuki Akazawa, Hiroshi Nakagawa, 山川允仁, 鈴木結加里 and Tomonori Iwasaki : 長期管理中の9pトリソミー症候群の1例, The Japanese Journal of Pediatric Dentistry, Vol.61, 213, Apr. 2023.
4.	Kimiko Ueda Yamaguchi, Yuki Akazawa, Keita Kawarabayashi, Aya Miyazaki, Takamasa Kitamura, Tomokazu Hasegawa, Hiroshi Nakagawa and Tomonori Iwasaki : 当院小児摂食嚥下外来における初診時調査, The Japanese Journal of Pediatric Dentistry, Vol.61, 53-54, Feb. 2023.
5.	Yuki Akazawa, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa and Tomonori Iwasaki : 習癖による咬傷に対してマウスピースを使用した1例, The Japanese Journal of Pediatric Dentistry, Vol.60, 63-64, Feb. 2022.
6.	Rie Niji, Kimiko Ueda Yamaguchi, Yuki Akazawa, Takamasa Kitamura and Tomonori Iwasaki : Streptococcus mutans定着時期に低濃度フッ素化合物を応用した小児の5歳時の齲蝕罹患状況, The Japanese Journal of Pediatric Dentistry, Vol.60, No.2, 55-56, Feb. 2022. (Keyword) Streptococcus mutans う蝕(予防,予後) *フッ素(治療的利用) ヒト乳児(1∼23ヶ月) 幼児(2∼5) 歯学
7.	Tomonori Iwasaki, Kei Maeo, Wakana Kohira, Takamasa Kitamura, Keita Kawarabayashi, Aya Miyazaki, Yuki Akazawa, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa and Tomokazu Hasegawa : 小児閉塞性睡眠時無呼吸への対応と展望, 徳島県小児保健協会総会並びに第62回講演会, 3, Sep. 2021.
8.	黒厚子璃佳, Tomokazu Hasegawa, Yuki Akazawa and Tsutomu Iwamoto : 歯髄細胞におけるFGF2によるJNK経路を介したCCL11の発現抑制, The Japanese Journal of Pediatric Dentistry, Vol.59, 117, Jun. 2021.
9.	Riri Kumazawa, Yuki Moriya, Tomokazu Hasegawa, Yuki Akazawa and Tsutomu Iwamoto : 歯根膜細胞における低酸素環境下でのエピジェネティクス制御の解析, 徳島県歯科医学大会, 36, Feb. 2020.
10.	Yuki Akazawa, Kokoro Iwata, Rika Kurogohshi, Keita Kawarabayashi, Aya Miyazaki, 藤島史帆, Hiroki Mori, Asuna Sugimoto, Takamasa Kitamura, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 本院小児歯科の初診患者の動向と主訴の変遷について, 徳島県歯科医学大会, 29, Feb. 2020.
11.	Kokoro Iwata, Yuki Akazawa and Tsutomu Iwamoto : 下顎左側乳犬歯が早期脱落した4歳男児の1例, 第38回日本小児歯科学会中四国地方会大会および総会, Nov. 2019.
12.	Asuna Sugimoto, Yuki Akazawa, Keita Kawarabayashi and Tsutomu Iwamoto : 口腔機能発達不全症児に対する口腔筋機能療法(MFT)の有用性, 第7回日本小児診療多職種研究会, Nov. 2018.
13.	Asuna Sugimoto, Aya Miyazaki, Keita Kawarabayashi, Kokoro Iwata, Rika Kurogohshi, Yuki Akazawa, Tomokazu Hasegawa, Kimiko Ueda Yamaguchi, Takamasa Kitamura, Hiroshi Nakagawa and Tsutomu Iwamoto : 象牙芽細胞におけるピエゾ型イオンチャネル1(PIEZO1)の発現とその役割, 第37回日本小児歯科学会中四国地方会大会, Nov. 2018.
14.	黒厚子璃佳, Asuna Sugimoto, Kokoro Iwata, Aya Miyazaki, Keita Kawarabayashi, 藤島史帆, Yuki Akazawa, Takamasa Kitamura, Kimiko Ueda Yamaguchi, Rie Niji, Tomokazu Hasegawa, Hiroshi Nakagawa and Tsutomu Iwamoto : 当科における5年間の障害児の初診時実態調査, 第37回日本小児歯科学会中四国地方会大会, Nov. 2018.
15.	Kokoro Iwata, Asuna Sugimoto, Rika Kurogohshi, Keita Kawarabayashi, Aya Miyazaki, 藤島史帆, Takamasa Kitamura, Yuki Akazawa, Rie Niji, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 当科における初診患者の実態調査~平成における30年間の変遷~, 第37回日本小児歯科学会中四国地方会大会, Nov. 2018.
16.	Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 外傷により両側顎関節完全脱臼を呈した5歳男児の1例, 第37回日本小児歯科学会中四国地方会大会, Nov. 2018.
17.	Rika Kurogohshi, Tomokazu Hasegawa, Yuki Akazawa, Asuna Sugimoto, Keita Kawarabayashi, Kimiko Ueda Yamaguchi and Tsutomu Iwamoto : 乳歯歯髄細胞におけるExotaxin-1の発現調節機構の解析, The Japanese Journal of Pediatric Dentistry, Vol.56, No.2, 215, May 2018.
18.	黒厚子璃佳, Yuki Akazawa, Tomokazu Hasegawa, Asuna Sugimoto, Kimiko Ueda Yamaguchi and Tsutomu Iwamoto : 乳歯歯髄細胞におけるケモカインCCL11の発現解析, The Japanese Journal of Pediatric Dentistry, Vol.56, No.1, 154, Nov. 2017.
19.	Asuna Sugimoto, Yuki Akazawa, Keita Kawarabayashi, Aya Miyazaki, Kimiko Ueda Yamaguchi, Takamasa Kitamura and Tsutomu Iwamoto : 当科における口腔筋機能療法(MFT)の効果と有用性について, The Japanese Journal of Pediatric Dentistry, Vol.56, No.1, 155, Nov. 2017.
20.	Keita Kawarabayashi, Yuki Akazawa, Asuna Sugimoto, Kimiko Ueda Yamaguchi, Takamasa Kitamura and Tsutomu Iwamoto : 齲蝕罹患と生活習慣の相関とその解決課題, The Japanese Journal of Pediatric Dentistry, Vol.56, No.1, 158, Nov. 2017.
21.	宮嵜彩, Asuna Sugimoto, 井上秀人, Takamasa Kitamura, Kimiko Ueda Yamaguchi, 河原林啓太, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 当科における10年間の口唇口蓋裂児の実態調査, 第55回日本小児歯科学会大会, Vol.55, No.2, 183, May 2017.
22.	杉本明日菜, Yuki Akazawa, Tomokazu Hasegawa, 宮嵜彩, 河原林啓太 and Tsutomu Iwamoto : PIEZO1は歯髄細胞の分化運命決定に関与し，象牙芽細胞への分化を促進させる, The Japanese Journal of Pediatric Dentistry, Vol.55, No.2, 290, 2017.
23.	宮嵜彩, 杉本明日菜, 井上秀人, Takamasa Kitamura, 上田公子, 河原林啓太, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 当科における10年間の口唇口蓋裂児の実態調査, The Japanese Journal of Pediatric Dentistry, Vol.55, No.2, 183, 2017.
24.	Yuki Akazawa, Tomokazu Hasegawa, 帖佐直幸, 吉村善隆, 浅川剛吉, 杉本明日菜, 河原林啓太, 宮嵜彩, 石崎明 and Tsutomu Iwamoto : ヒト乳歯歯髄細胞のSDF-1発現調節機構におけるALK5の関与について, The Japanese Journal of Pediatric Dentistry, Vol.55, No.2, 291, 2017.
25.	Yuki Akazawa, Kimiko Ueda Yamaguchi, Tomokazu Hasegawa and Tsutomu Iwamoto : 低位下顎左側第二乳臼歯の再萌出を認めた1例, The Japanese Journal of Pediatric Dentistry, Vol.55, No.1, 141-142, Nov. 2016.
26.	Rie Niji, Kimiko Ueda Yamaguchi, Yuki Akazawa, Takamasa Kitamura, Tomokazu Hasegawa, 杉本明日菜 and Tsutomu Iwamoto : 歯の萌出時期に低濃度フッ素化合物を使用した小児の齲蝕罹患状況, The Japanese Journal of Pediatric Dentistry, Vol.54, No.1, 144, Oct. 2015.
27.	Kimiko Ueda Yamaguchi, Yuki Akazawa, Takamasa Kitamura, 杉本明日菜, Tomokazu Hasegawa, Hiroshi Nakagawa and Tsutomu Iwamoto : ヒト歯肉線維芽細胞におけるS-PRGフィラー抽出液による細胞遊走の促進, The Japanese Journal of Pediatric Dentistry, Vol.54, No.1, 145, Oct. 2015.
28.	Kimiko Ueda Yamaguchi, 益富由佳子, Rie Niji, Takamasa Kitamura, Aimi Yamamoto, Yuki Akazawa, 杉本明日菜, 白石真紀, Hiroshi Nakagawa, Tomokazu Hasegawa, Yukiko Kori and Tsutomu Iwamoto : Clinical survey of traumatic dental injuries in children at our outpatient clinic, The Japanese Journal of Pediatric Dentistry, Vol.53, No.2, 272, May 2015.
29.	Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 象牙質修復に関わる間葉系幹細胞の乳歯歯髄細胞由来SDF-1αによる制御, The Japanese Journal of Pediatric Dentistry, Vol.53, No.2, 221, 2015.
30.	Tomokazu Hasegawa, Yuki Akazawa, 吉村善隆, 帖佐直幸, 浅川剛吉, 杉本明日菜, Takamasa Kitamura, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa, 白石真紀, 石崎明 and Tsutomu Iwamoto : 乳歯歯根膜由来細胞のSDF-1αを介した歯周組織恒常性に関わる細胞間相互作用, The Japanese Journal of Pediatric Dentistry, Vol.53, No.2, 222, 2015.
31.	幸江真杉, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 先天性気管狭窄症に対する口腔内衛生管理の1例, The Japanese Journal of Pediatric Dentistry, Vol.53, No.1, 168, 2015.
32.	Takamasa Kitamura, Hiroshi Nakagawa, 杉本明日菜, Yuki Akazawa, Tomokazu Hasegawa and Tsutomu Iwamoto : 含歯性嚢胞の間葉系結合組織層が骨吸収に与える影響, 歯基礎誌抄録, 141, 2014.
33.	Hiroshi Nakagawa, Aimi Yamamoto, Yuki Akazawa, Takamasa Kitamura, Tomokazu Hasegawa and Tsutomu Iwamoto : p-HPPHを分子標的としたフェニトインによる歯肉増殖症に対する新規治療薬の開発, Journal of the Japanese Society for Disability and Oral Health, Vol.35, No.3, 478, 2014.
34.	Tomokazu Hasegawa, Yuki Akazawa, 帖佐直幸, 吉村善隆, 浅川剛吉, 石崎明 and Tsutomu Iwamoto : ヒト乳歯歯髄細胞におけるSDF-1の発現調節機構, 第55回歯科基礎医学会学術大会, Sep. 2013.
35.	Yuki Akazawa, Tomokazu Hasegawa, 帖佐直幸, 吉村善隆, 浅川剛吉, 石崎明 and Tsutomu Iwamoto : ヒト乳歯歯髄細胞由来の不死化細胞は骨および脂肪細胞への分化能を持つ, 第55回歯科基礎医学会学術大会, Sep. 2013.
36.	Hiroshi Nakagawa, Aimi Yamamoto, Yukiko Kori, Kimiko Ueda Yamaguchi, Yuki Akazawa, Takamasa Kitamura, Tomokazu Hasegawa and Tsutomu Iwamoto : 歯科治療恐怖症患者に応用した心理検査結果の経時的変化について, Journal of the Japanese Society for Disability and Oral Health, Vol.34, No.3, 375, Sep. 2013.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

間葉系幹細胞の骨芽細胞分化におけるメカノセンサー、メカノトランスダクションの解明 (Project/Area Number: 23K09436 )
小児鼻腔通気障害に対する歯科的治療法の開拓と治療効果の検討 (Project/Area Number: 22K10274 )
超遠心法による分画抽出血清のプロテオーム解析を利用した新規硬組織形成誘導法の開発 (Project/Area Number: 21K10162 )
Development of a new method for inducing hard tissue formation using the fraction of serum extracted by the ultracentrifugation. (Project/Area Number: 18K09834 )
Basic analysis and development of treatment method of periodontal disease in Down Syndrome focusing on the immune system (Project/Area Number: 17K17332 )
Analysis of intercellular network mechanism in tissue repair and homeostatic maintenance by dental pulp cells (Project/Area Number: 26861790 )
Search by Researcher Number (10646152)

Assistant Professor : Akazawa, Yuki

Research

Field of Study

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

Review, Commentary:

Proceeding of International Conference:

Proceeding of Domestic Conference:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)