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Research

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Field of Study

Life sciences [Zoological sciences]

Subject of Study

Book / Paper

Book:

1. Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines.,
Methods Mol Biol., 2023.
(DOI: 10.1007/978-1-0716-3016-7_22,   PubMed: 36773155)

Academic Paper (Judged Full Paper):

1. Thanh-Van Nguyen, Kim Lanh Thi Do, Qingyi Lin, Megumi Nagahara, Zhao Namula, Manita Wittayarat, Maki Hirata, Takeshige Otoi and Fuminori Tanihara :
Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00869-4,   PubMed: 38485817)
2. Thanh-Van Nguyen, Koki Takebayashi, Kim Lanh Thi Do, Zhao Namula, Manita Wittayarat, Megumi Nagahara, Maki Hirata, Takeshige Otoi and Fuminori Tanihara :
Generation of allogenic chimera carrying mutations in PDX1 and TP53 genes via phytohemagglutinin-mediated blastomere aggregation in pigs.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00870-x,   PubMed: 38379097)
3. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos.,
Animal Science Journal, Vol.94, No.1, e13878, 2023.
(DOI: 10.1111/asj.13878,   PubMed: 37818780)
4. Hisayoshi Omori, Junko Chikamoto, Megumi Nagahara, Maki Hirata and Takeshige Otoi :
Evaluating variations in bilirubin glucuronidation activity by protease inhibitors in canine and human primary hepatocytes cultured in a 3D culture system.,
Toxicology In Vitro, Vol.93, 2023.
(DOI: 10.1016/j.tiv.2023.105689,   PubMed: 37660998)
5. Chommanart Thongkittidilok, Maki Hirata, Qingyi Lin, Nanaka Torigoe, Bin Liu, Yoko Sato, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study.,
Veterinary Medicine International, Vol.2023, 7000858, 2023.
(DOI: 10.1155/2023/7000858,   PubMed: 37609627,   Elsevier: Scopus)
6. Fuminori Tanihara, Maki Hirata, Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Qingyi Lin, Koki Takebayashi, Hiromasa Hara, Megumi Nagahara and Takeshige Otoi :
GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system.,
Molecular Biology Reports, Vol.50, No.6, 5049-5057, 2023.
(DOI: 10.1007/s11033-023-08388-3,   PubMed: 37101010)
7. Thanh-Van Nguyen, Kim Lanh Thi Do, Zhao Namula, Qingyi Lin, Nanaka Torigoe, Megumi Nagahara, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Development and Genome Mutation of Bovine Zygotes Vitrified Before and After Genome Editing via Electroporation,
Cryo Letters, Vol.44, No.2, 118-122, 2023.
(DOI: 10.54680/fr23210110612,   Elsevier: Scopus)
8. Fuminori Tanihara, Maki Hirata, Zhao Namula, Kim Lanh Thi Do, Naoaki Yoshimura, Qingyi Lin, Koki Takebayashi, Tetsushi Sakuma, Takashi Yamamoto and Takeshige Otoi :
Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN,
Frontiers in Cell and Developmental Biology, Vol.11, 2023.
(DOI: 10.3389/fcell.2023.884340,   PubMed: 36711037)
9. Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Nanaka Torigoe, Bin Liu, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Efficiency of genetic modification in gene-knockout sperm-derived zygotes followed by electroporation of guide RNA targeting the same gene.,
Animal Science Journal, Vol.94, No.1, e13842, 2023.
(DOI: 10.1111/asj.13842,   PubMed: 37218074,   Elsevier: Scopus)
10. Anh Quynh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Kim Lanh Thi Do and Takeshige Otoi :
Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.,
Veterinary World, Vol.15, No.9, 2210-2216, 2022.
(Tokushima University Institutional Repository: 117906,   DOI: 10.14202/vetworld.2022.2210-2216,   PubMed: 36341066)
11. Maki Hirata, Miki Matsuoka, Takuma Hashimoto, Takamichi Oura, Yo Ohnuki, Chika Yoshida, Ayaka Minemura, Daiki Miura, Kentaro Oka, Motomichi Takahashi and Fumiki Morimatsu :
Supplemental Clostridium butyricum MIYAIRI 588 Affects Intestinal Bacterial Composition of Finishing Pigs.,
Microbes and Environments, Vol.37, No.3, ME22011, 2022.
(DOI: 10.1264/jsme2.ME22011,   PubMed: 36155363)
12. Hisayoshi Omori, Junko Chikamoto, Takayuki Hirano, Kazuhiko Besshi, Naoaki Yoshimura, Maki Hirata and Takeshige Otoi :
Comparative analysis of bilirubin glucuronidation activity in canine and human primary hepatocytes using a 3D culture system.,
In Vitro Cellular & Developmental Biology. Animal, Vol.58, No.8, 712-718, 2022.
(DOI: 10.1007/s11626-022-00711-9,   PubMed: 35913527)
13. Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Maki Hirata, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Kim Lanh Thi Do, Fuminori Tanihara and Takeshige Otoi :
Gene editing in porcine embryos using a combination of electroporation and transfection methods.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.10, 1136-1142, 2022.
(DOI: 10.1111/rda.14184,   PubMed: 35699358)
14. Megumi Shimazaki, Manita Wittayarat, Rentsenkhand Sambuu, Asami Sugita, Masaki Kawaguchi, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi, Takeshige Otoi and Yoko Sato :
Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle-yaks affects spermatogenic cell fate and sterility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.9, 999-1006, 2022.
(DOI: 10.1111/rda.14166,   PubMed: 35614560,   Elsevier: Scopus)
15. Zhao Namula, Manita Wittayarat, Kim Lanh Thi Do, Thanh Nguyen Van, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system,
Veterinary and Animal Science, Vol.16, 2022.
(Tokushima University Institutional Repository: 117723,   DOI: 10.1016/j.vas.2022.100241,   PubMed: 35265771)
16. Zhao Namula, Anh Quynh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Kim Lanh Thi Do, Fuminori Tanihara and Takeshige Otoi :
Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection.,
Veterinary World, Vol.15, No.2, 496-501, 2022.
(Tokushima University Institutional Repository: 117724,   DOI: 10.14202/vetworld.2022.496-501,   PubMed: 35400948)
17. Qingyi Lin, Anh Quynh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.5, 556-563, 2022.
(DOI: 10.1111/rda.14095,   PubMed: 35137478)
18. Qingyi Lin, Anh Quynh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
Short-term preservation of porcine zygotes at ambient temperature using a chemically defined medium.,
Animal Science Journal, Vol.93, No.1, 2022.
(DOI: 10.1111/asj.13711,   PubMed: 35373427)
19. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Taro Mito, Sayuri Tomonari, Fuminori Tanihara and Takeshige Otoi :
Generation of mutant pigs by lipofection-mediated genome editing in embryos.,
Scientific Reports, Vol.11, No.1, 23806, 2021.
(Tokushima University Institutional Repository: 116977,   DOI: 10.1038/s41598-021-03325-5,   PubMed: 34903813)
20. Chommanart Thongkittidilok, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Lanh Thi Kim Do, Zhao Namula, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Effects of individual or in-combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.3, 314-320, 2021.
(DOI: 10.1111/rda.14063,   PubMed: 34862995)
21. Praopilas Phakdeedindan, Manita Wittayarat, Theerawat Tharasanit, Mongkol Techakumphu, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Takeshige Otoi and Yoko Sato :
Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.3, 304-313, 2021.
(DOI: 10.1111/rda.14061,   PubMed: 34854139)
22. Takeshi Kikuchi, Masuhiro Nishimura, Maki Hirata, Fuminori Tanihara, Natsuki Komori, Makoto Tanaka, Osamu Sawamoto, Takeshige Otoi and Shinichi Matsumoto :
Development and characterization of Gal KO porcine bone marrow-derived mesenchymal stem cells.,
Xenotransplantation, Vol.28, No.6, e12717, 2021.
(DOI: 10.1111/xen.12717,   PubMed: 34730861)
23. Zhao Namula, Maki Hirata, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok and Takeshige Otoi :
Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes.,
Veterinary Medicine and Science, Vol.8, No.1, 164-169, 2021.
(Tokushima University Institutional Repository: 116985,   DOI: 10.1002/vms3.659,   PubMed: 34674375)
24. Qingyi Lin, Anh Quynh Le, Koki Takebayashi, Chommanart Thongkittidilok, Manita Wittayarat, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events.,
BMC Research Notes, Vol.14, No.1, 389, 2021.
(Tokushima University Institutional Repository: 116892,   DOI: 10.1186/s13104-021-05800-8,   PubMed: 34627381)
25. Naoaki Yoshimura, Masayasu Taniguchi, Tsukasa Terazono, Tetsushi Ono, Mitsuhiro Takagi, Yoko Sato, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG.,
Veterinary Medicine and Science, Vol.7, No.5, 1569-1574, 2021.
(Tokushima University Institutional Repository: 116673,   DOI: 10.1002/vms3.552,   PubMed: 34137199)
26. Zhao Namula, Yasuhiro Isumi, Yoko Sato, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok and Takeshige Otoi :
Improvement of the in vitro fertilization and embryo development using frozen-thawed spermatozoa of microminipigs.,
Archives Animal Breeding, Vol.64, No.1, 265-271, 2021.
(Tokushima University Institutional Repository: 116482,   DOI: 10.5194/aab-64-265-2021,   PubMed: 34189254)
27. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.,
International Journal of Molecular Sciences, Vol.22, No.5, 2249, 2021.
(Tokushima University Institutional Repository: 116340,   DOI: 10.3390/ijms22052249,   PubMed: 33668187,   Elsevier: Scopus)
28. Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Le Anh, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara and Takeshige Otoi :
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.,
Animals : An Open Access Journal from MDPI, Vol.11, No.2, 2021.
(Tokushima University Institutional Repository: 116270,   DOI: 10.3390/ani11020578,   PubMed: 33672168,   Elsevier: Scopus)
29. Anh Quynh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata and Takeshige Otoi :
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.,
BMC Research Notes, Vol.14, No.1, 7, 2021.
(Tokushima University Institutional Repository: 116277,   DOI: 10.1186/s13104-020-05412-8,   PubMed: 33407863)
30. Kim Thi Lanh Do, Manita Wittayarat, Yoko Sato, Kaywalee Chatdarong, Theerawat Tharasanit, Mongkol Techakumphu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
Comparison of blastocyst development between cat-cow and cat-pig interspecies somatic cell nuclear transfer embryos under the treatment of Trichostatin A.,
Biology bulletin of the Russian Academy of Sciences, Vol.48, 107-117, 2021.
(DOI: 10.1134/S1062359021020035)
31. Nguyen Nhien Thi, Wittayarat Manita, Zhao Namula, Sato Yoko, Anh Le Quynh, Lin Qingyi, Takebayashi Koki, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Chlorogenic acid and insulin-transferrin-selenium supplementation during in vitro maturation enhances the developmental competence of interspecies chimera blastocysts following cell injection.,
Journal of Applied Animal Research, Vol.49, No.1, 486-491, 2021.
(DOI: 10.1080/09712119.2021.2011295,   Elsevier: Scopus)
32. Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, T Nhien Nguyen, A Quynh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.,
Animal Science Journal, Vol.92, No.1, e13534, 2021.
(DOI: 10.1111/asj.13534,   PubMed: 33638256)
33. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Le Anh Quynh, Manita Wittayarat, Mokhamad Fahrudin, Takayuki Hirano and Takeshige Otoi :
Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes.,
Animal Biotechnology, Vol.32, 147-154, 2021.
(DOI: 10.1080/10495398.2019.1668801,   PubMed: 31558095)
34. Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi and Takeshige Otoi :
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.8, 614-621, 2020.
(DOI: 10.1007/s11626-020-00507-9,   PubMed: 32978715)
35. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi :
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes.,
BMC Biotechnology, Vol.20, No.1, 40, 2020.
(Tokushima University Institutional Repository: 115881,   DOI: 10.1186/s12896-020-00638-7,   PubMed: 32811500)
36. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Thi Nhien Nguyen, Koki Takebayashi, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation.,
Molecular Biology Reports, Vol.47, No.7, 5073-5079, 2020.
(DOI: 10.1007/s11033-020-05576-3,   PubMed: 32519310)
37. Takayuki Hirano, Maki Hirata, Shigeyuki Fujimoto, Thi Nhien Nguyen, Le Anh Quynh, Fuminori Tanihara and Takeshige Otoi :
Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.4, 277-280, 2020.
(DOI: 10.1007/s11626-020-00451-8,   PubMed: 32394241)
38. Fuminori Tanihara, Maki Hirata, Nhien Nguyen Thi, Le Anh Quynh, Takayuki Hirano and Takeshige Otoi :
Generation of viable PDX1 gene-edited founder pigs as providers of nonmosaics.,
Molecular Reproduction and Development, Vol.87, No.4, 471-481, 2020.
(DOI: 10.1002/mrd.23335,   PubMed: 32166879)
39. T N Nguyen, Maki Hirata, Fuminori Tanihara, Y Sato, Z Namula, Le Trong Quang, M Wittayarat, M Fahrudin and Takeshige Otoi :
In vitro Development of Zona Pellucida-free Porcine Zygotes Cultured Individually after Vitrification.,
Cryo Letters, Vol.41, No.2, 86-91, 2020.
(PubMed: 33988658)
40. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi :
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020.
(DOI: 10.1111/rda.13609,   PubMed: 31858644)
41. Namula Zhao, Yoko Sato, Manita Wittayarat, Quynh Le Anh, Thi Nhien Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Curcumin supplementation in maturation medium improves the maturation, fertilisation, and developmental competence of porcine oocytes.,
Acta Veterinaria Hungarica, Vol.68, No.3, 298-304, 2020.
(DOI: 10.1556/004.2020.00041,   PubMed: 33221737)
42. A Quynh Le, Maki Hirata, T Nhien Nguyen, Koki Takebayashi, Manita Wittayarat, Yoko Sato, Zhao Namula, Masahiro Nii, Fuminori Tanihara and Takeshige Otoi :
Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes.,
Animal Science Journal, Vol.91, No.1, e13386, 2020.
(DOI: 10.1111/asj.13386,   PubMed: 32512638)
43. Maki Hirata, Manita Wittayarat, Takayuki Hirano, Thi Nhien Nguyen, Le Anh Quynh, Zhao Namula, Mokhamad Fahrudin, Fuminori Tanihara and Takeshige Otoi :
The relationship between embryonic development and the efficiency of target mutations in porcine endogenous retroviruses (PERVs) pol genes in porcine embryos.,
Animals : An Open Access Journal from MDPI, Vol.9, No.9, E593, 2019.
(Tokushima University Institutional Repository: 114984,   DOI: 10.3390/ani9090593,   PubMed: 31443357)
44. Namula Zhao, Manita Wittayarat, Maki Hirata, Hirano Takayuki, Nguyen Thi Nhien, Le Anh Quynh, Fahrudin Mokhamad, Fuminori Tanihara and Takeshige Otoi :
Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.8, 598-603, 2019.
(DOI: 10.1007/s11626-019-00385-w,   PubMed: 31297696)
45. Fuminori Tanihara, Maki Hirata, Morikawa Shigeki, Thi Nhien Nguyen, Le Anh Quynh, Hirano Takayuki, Fukumi Yoshiyuki, Abe Toshiaki and Takeshige Otoi :
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts,
The Journal of Reproduction and Development, Vol.65, No.5, 475-479, 2019.
(Tokushima University Institutional Repository: 115540,   DOI: 10.1262/jrd.2019-049,   PubMed: 31178553)
46. Fuminori Tanihara, Maki Hirata, Satoru Iizuka, Shinya Sairiki, Masahiro Nii, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
Relationship among ovarian follicular status, developmental competence of oocytes, and anti-M llerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.,
Animal Science Journal, Vol.90, No.6, 712-718, 2019.
(DOI: 10.1111/asj.13200,   PubMed: 30977253)
47. Nhien Nguyen Thi, Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.54, No.5, 750-755, 2019.
(DOI: 10.1111/rda.13417,   PubMed: 30788874,   Elsevier: Scopus)
48. Zhao Namula, Fuminori Tanihara, Manita Wittayarat, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.,
Acta Veterinaria Hungarica, Vol.67, No.1, 106-114, 2019.
(DOI: 10.1556/004.2019.012,   PubMed: 30922097)
49. Maki Hirata, Fuminori Tanihara, Manita Wittayarat, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, Zhao Namula, Masahiro Nii and Takeshige Otoi :
Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.4, 237-242, 2019.
(DOI: 10.1007/s11626-019-00338-3,   PubMed: 30820813)
50. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh LE, Takayuki Hirano and Takeshige Otoi :
Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos.,
The Journal of Reproduction and Development, Vol.65, No.3, 209-214, 2019.
(Tokushima University Institutional Repository: 115541,   DOI: 10.1262/jrd.2018-116,   PubMed: 30726783,   Elsevier: Scopus)
51. Fuminori Tanihara, Maki Hirata, Nhien T. Nguyen, Quynh A. Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.,
Animal Science Journal, Vol.90, No.1, 55-61, 2018.
(DOI: 10.1111/asj.13129,   PubMed: 30368976)
52. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.,
PLoS ONE, Vol.13, No.10, e0206360, 2018.
(Tokushima University Institutional Repository: 112917,   DOI: 10.1371/journal.pone.0206360,   PubMed: 30352075)
53. Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Mitsuhiro Takagi, Tsukasa Terazono and Takeshige Otoi :
Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.,
Veterinary Medicine and Science, 2018.
(Tokushima University Institutional Repository: 115827,   DOI: 10.1002/vms3.121,   PubMed: 30273973)
54. Maki Hirata, Fuminori Tanihara, Taniguchi Masayasu, Takagi Mitsuhiro, Terazono Tsukasa and Takeshige Otoi :
Viability of canine ovaries autografted to different peripheral sites,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, No.2, 140-148, 2018.
(Elsevier: Scopus)
55. Zhao Namula, Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Nhien Nguyen Thi, Takayuki Hirano, Masahiro Nii and Takeshige Otoi :
Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.,
Reproduction in Domestic Animals = Zuchthygiene, 2018.
(Tokushima University Institutional Repository: 116076,   DOI: 10.1111/rda.13288,   PubMed: 30053311)
56. Thanh-Van Nguyen, Manita Wittayarat, Kim Lanh Thi Do, Van Thanh Nguyen, Masahiro Nii, Zhao Namula, Toshiki Kunihara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.,
Animal Science Journal, Vol.89, No.8, 1207-1213, 2018.
(Tokushima University Institutional Repository: 116078,   DOI: 10.1111/asj.13049,   PubMed: 29806122)
57. Fuminori Tanihara, Maki Hirata, Thi Nguyen Nhien, Takayuki Hirano, Toshiki Kunihara and Takeshige Otoi :
Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation.,
The Journal of Veterinary Medical Science, Vol.80, No.6, 1007-1011, 2018.
(Tokushima University Institutional Repository: 115574,   DOI: 10.1292/jvms.18-0131,   PubMed: 29769451)
58. V T Nguyen, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Katsutoshi Nishio, T Do L Kim, V T Nguyen, M Nii and Takeshige Otoi :
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.,
Cryo Letters, Vol.39, No.2, 131-136, 2018.
(PubMed: 29734422,   Elsevier: Scopus)
59. Megumi Shimazaki, Urasoko Saki, Tanaka Masako, Sato Yoko, Fuminori Tanihara, Maki Hirata, Taniguchi Masayasu, Takagi Mitsuhiro and Takeshige Otoi :
Effects of Orvus Es Paste (OEP) on the viability of bull spermatozoa after double freezing and thawing.,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, 32-38, 2018.
(CiNii: 1010003825435470592,   Elsevier: Scopus)
60. Katsutoshi Nishio, Fuminori Tanihara, T-V Nguyen, Toshiki Kunihara, M Nii, Maki Hirata, Tatsuya Takemoto and Takeshige Otoi :
Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.53, No.2, 313-318, 2017.
(Tokushima University Institutional Repository: 116077,   DOI: 10.1111/rda.13106,   PubMed: 29135047)
61. Nobuyuki Nakajima, Tetsuro Tamaki, Maki Hirata, Shuichi Soeda, Masahiro Nitta, Akio Hoshi and Toshiro Terachi :
Purified human skeletal muscle-derived stem cells enhance the repair and regeneration in the damaged urethra.,
Transplantation, Vol.101, No.10, 2312-2320, 2017.
(DOI: 10.1097/TP.0000000000001613,   PubMed: 28027190)
62. Tetsuro Tamaki, Maki Hirata, Nobuyuki Nakajima, Kosuke Saito, Hiroyuki Hashimoto, Shuichi Soeda, Yoshiyasu Uchiyama and Masahiko Watanabe :
A long-gap peripheral nerve injury therapy using human skeletal muscle-derived stem cells (Sk-SCs): An achievement of significant morphological, numerical and functional recovery.,
PLoS ONE, Vol.11, No.11, e0166639, 2016.
(Tokushima University Institutional Repository: 115242,   DOI: 10.1371/journal.pone.0166639,   PubMed: 27846318)
63. Hiroyuki Hashimoto, Tetsuro Tamaki, Maki Hirata, Yoshiyasu Uchiyama, Masato Sato and Joji Mochida :
Reconstitution of the complete rupture in musculotendinous junction using skeletal muscle-derived multipotent stem cell sheet-pellets as a "bio-bond".,
PeerJ, Vol.4, e2231, 2016.
(DOI: 10.7717/peerj.2231,   PubMed: 27547541)
64. Kosuke Saito, Tetsuro Tamaki, Maki Hirata, Hiroyuki Hashimoto, Kenei Nakazato, Nobuyuki Nakajima, Akihito Kazuno, Akihiro Sakai, Masahiro Iida and Kenji Okami :
Reconstruction of multiple facial nerve branches using skeletal muscle-derived multipotent stem cell sheet-pellet transplantation.,
PLoS ONE, Vol.10, No.9, e0138371, 2015.
(DOI: 10.1371/journal.pone.0138371,   PubMed: 26372044)
65. Tetsuro Tamaki, Yoshiyasu Uchiyama, Maki Hirata, Hiroyuki Hashimoto, Nobuyuki Nakajima, Kosuke Saito, Toshiro Terachi and Joji Mochida :
Therapeutic isolation and expansion of human skeletal muscle-derived stem cells for the use of muscle-nerve-blood vessel reconstitution.,
Frontiers in Physiology, Vol.6, 165, 2015.
(DOI: 10.3389/fphys.2015.00165,   PubMed: 26082721)
66. Tetsuro Tamaki, Maki Hirata and Yoshiyasu Uchiyama :
Qualitative alteration of peripheral motor system begins prior to appearance of typical sarcopenia syndrome in middle-aged rats.,
Frontiers in Aging Neuroscience, Vol.6, 296, 2014.
(DOI: 10.3389/fnagi.2014.00296,   PubMed: 25400579)
67. Tetsuro Tamaki, Maki Hirata, Shuichi Soeda, Nobuyuki Nakajima, Kosuke Saito, Kenei Nakazato, Yoshinori Okada, Hiroyuki Hashimoto, Yoshiyasu Uchiyama and Joji Mochida :
Preferential and comprehensive reconstitution of severely damaged sciatic nerve using murine skeletal muscle-derived multipotent stem cells.,
PLoS ONE, Vol.9, No.3, e91257, 2014.
(DOI: 10.1371/journal.pone.0091257,   PubMed: 24614849)
68. Tetsuro Tamaki, Shuichi Soeda, Hiroyuki Hashimoto, Kosuke Saito, Akihiro Sakai, Nobuyuki Nakajima, Maki Masuda, Nahoko Fukunishi, Yoshiyasu Uchiyama, Toshiro Terachi and Joji Mochida :
3D reconstitution of nerve-blood vessel networks using skeletal muscle-derived multipotent stem cell sheet pellets.,
Regenerative Medicine, Vol.8, No.4, 437-451, 2013.
(DOI: 10.2217/rme.13.30,   PubMed: 23826698)
69. Tetsuro Tamaki, Kayoko Tono, Yoshiyasu Uchiyama, Yoshinori Okada, Maki Masuda, Shuichi Soeda, Masahiro Nitta and Akira Akatsuka :
Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro.,
Cell and Tissue Research, Vol.344, No.1, 147-168, 2011.
(DOI: 10.1007/s00441-010-1127-9,   PubMed: 21274567)
70. Masahiro Nitta, Tetsuro Tamaki, Kayoko Tono, Yoshinori Okada, Maki Masuda, Akira Akatsuka, Akio Hoshi, Yukio Usui and Toshiro Terachi :
Reconstitution of experimental neurogenic bladder dysfunction using skeletal muscle-derived multipotent stem cells.,
Transplantation, Vol.89, No.9, 1043-1049, 2010.
(DOI: 10.1097/TP.0b013e3181d45a7f,   PubMed: 20150836)
71. Tetsuro Tamaki, Yoshiyasu Uchiyama, Yoshinori Okada, Kayoko Tono, Maki Masuda, Masahiro Nitta, Akio Hoshi and Akira Akatsuka :
Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo.,
Stem Cells and Development, Vol.19, No.4, 503-512, 2010.
(DOI: 10.1089/scd.2009.0179,   PubMed: 19634996)
72. Kazunari Yamashita, Atsushi Suzuki, Yoshinori Satoh, Mariko Ide, Yoshiko Amano, Maki Masuda-Hirata, K Yukiko Hayashi, Keisuke Hamada, Kazuhiro Ogata and Shigeo Ohno :
The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b.,
Biochemical and Biophysical Research Communications, Vol.391, No.1, 812-817, 2009.
(DOI: 10.1016/j.bbrc.2009.11.144,   PubMed: 19945424)
73. Maki Masuda-Hirata, Atsushi Suzuki, Yoshiko Amano, Kazunari Yamashita, Mariko Ide, Tomoyuki Yamanaka, Michihiro Sakai, Michihiro Imamura and Shigeo Ohno :
Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex.,
Genes to Cells, Vol.14, No.7, 835-850, 2009.
(DOI: 10.1111/j.1365-2443.2009.01315.x,   PubMed: 19549170)
74. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Masahiro Nitta, Akio Hoshi and Akira Akatsuka :
Skeletal muscle-derived CD34+/45- and CD34-/45- stem cells are situated hierarchically upstream of Pax7+ cells.,
Stem Cells and Development, Vol.17, No.4, 653-667, 2008.
(DOI: 10.1089/scd.2008.0070,   PubMed: 18554087)
75. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Mika Wada, Akio Hoshi and Akira Akatsuka :
Synchronized reconstitution of muscle fibers, peripheral nerves and blood vessels by murine skeletal muscle-derived CD34(-)/45 (-) cells.,
Histochemistry and Cell Biology, Vol.128, No.4, 349-360, 2007.
(DOI: 10.1007/s00418-007-0331-5,   PubMed: 17762938)
76. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Mika Wada, Akio Hoshi, Tetsuya Ishikawa and Akira Akatsuka :
Clonal multipotency of skeletal muscle-derived stem cells between mesodermal and ectodermal lineage.,
Stem Cells, Vol.25, No.9, 2283-2290, 2007.
(DOI: 10.1634/stemcells.2006-0746,   PubMed: 17588936)
77. Atsushi Suzuki, Maki Hirata, Katsusi Kamimura, Rika Maniwa, Tomoyuki Yamanaka, Keiko Mizuno, Masaru Kishikawa, Hiroko Hirose, Yoshiko Amano, Natsuko Izumi, Yoshihiro Miwa and Shigeo Ohno :
aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity.,
Current Biology, Vol.14, No.16, 1425-1435, 2004.
(DOI: 10.1016/j.cub.2004.08.021,   PubMed: 15324659)
78. Mitsuhiro Kurata, Maki Hirata, Shoji Watabe, Masashi Miyake, Y Susumu Takahashi and Yoshimi Yamamoto :
Expression, purification, and inhibitory activities of mouse cytotoxic T-lymphocyte antigen-2alpha.,
Protein Expression and Purification, Vol.32, No.1, 119-125, 2003.
(DOI: 10.1016/S1046-5928(03)00222-5,   PubMed: 14680948)

Review, Commentary:

1. Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
ゲノム編集技術による遺伝子改変家畜の作出,
Journal of Mammalian Ova Research, Vol.39, 17-26, Jan. 2022.
(CiNii: 1050858518835823232)
2. Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Current status of the application of gene editing in pigs.,
The Journal of Reproduction and Development, Vol.67, No.3, 177-187, Apr. 2021.
(Tokushima University Institutional Repository: 116670,   DOI: 10.1262/jrd.2021-025,   PubMed: 33840678)

Proceeding of International Conference:

1. Lin Qingyi, Maki Hirata, K Takebayashi, N. Torigoe, M. Nagahara and Takeshige Otoi :
Comparison of chemically mediated CRISPR/Cas9 gene-editing systems using different transfection mechanisms on the mutation of porcine embryos.,
The 17th Transgenic Technology Meeting (TT2022), Helsinki, Finland, Sep. 2022.
2. Maki Hirata, Matsuoka Miki, Hashimoto Takuma, Oura Takamichi, Ohnuki Yo, Yoshida Chika, Minemura Ayaka, Miura Daiki, Oka Kentaro, Takahashi Motomichi and Fumiki Morimatsu :
Effect of Clostridium butyricum MIYAIRI 588 supplementation on the intestinal microbiota and meat quality of fattening pigs.,
68th International Congress of Meat Science and Technology (ICoMST2022), Kobe, Aug. 2022.
3. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
4. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Hirano Takayuki, Le Anh Quynh and Takeshige Otoi :
Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
5. Le Anh Quynh, Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Hirano Takayuki and Takeshige Otoi :
Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
6. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.

Proceeding of Domestic Conference:

1. Maki Hirata, NIIMI Nagisa, Ryoko Buma, Mikami Nana, Yamasaki Sakae and Fumiki Morimatsu :
食品ロス低減に向けた食肉・水産加工品の腐敗菌ライブラリー構築に関する取り組み -第二報 食肉加工品由来腐敗菌の網羅的調査-,
日本防菌防黴学会 第50回年次大会, Sep. 2023.
2. 吉田 知加, Maki Hirata, 大貫 燿, 扇 隆介, NIWA Rikuto, HASHIMOTO Takuma, 三浦 大輝, 峯村 采花, 岡 健太郎, 高橋 志達 and Fumiki Morimatsu :
フィターゼと酪酸菌を配合した飼料添加物が育成期仔豚の生産性,骨密度および腸内細菌叢に与える影響,
第72回関西畜産学会大会(岡山大会), Oct. 2022.
3. Maki Hirata, FUJII Takeru, NIIMI Nagisa, 倉園 久生, 山崎 栄樹, 奥村 香世, 近藤 有華, Ryoko Buma and Fumiki Morimatsu :
食肉加工品由来腐敗菌ライブラリ―構築に対する取り組み,
日本防菌防黴学会 第49回年次大会, Sep. 2022.
4. 大貫 燿, Maki Hirata, 松岡 美樹, 吉田 知加, 扇 隆介, 小浦 考修, HASHIMOTO Takuma, 峯村 采花, 三浦 大輝, 岡 健太郎, 高橋 志達 and Fumiki Morimatsu :
完全閉鎖型豚舎における肥育後期ブタ腸内細菌叢と増体量との関連についての解析,
日本畜産学会第130回大会, Sep. 2022.
5. HASHIMOTO Takuma, Maki Hirata, 松岡 美樹, 大貫 燿, 吉田 知加, 扇 隆介, NIWA Rikuto, 岡 健太郎, 高橋 志達 and Fumiki Morimatsu :
寒暖差環境下における豚へのClostridium butyricum MIYAIRI 588給与の影響,
日本畜産学会第130回大会, Sep. 2022.
6. 小浦 考修, 高垣 堅太郎, Maki Hirata and Fumiki Morimatsu :
マイクロミニピッグの初期発達に関する研究,第165回日本獣医学会学術集会,
第165回日本獣医学会学術集会, Sep. 2022.
7. Q Lin, Quynh Anh Le, Manita Wittayarat, Zhao Namula, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Kim Lanh Thi Do and Takeshige Otoi :
Triple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.,
第7回ゲノム編集学会, Jun. 2022.
8. Maki Hirata, Tetsuya Ikemoto, Kazunori Tokuda, Shohei Okikawa, 竹島 雅之, Fumiki Morimatsu and Mitsuo Shimada :
糖尿病モデルマイクロミニブタの確立,
第69回日本実験動物学会総会, May 2022.
9. 大貫 耀, Maki Hirata, 松岡 美樹, 吉田 知加, 小浦 考修, 橋本 託真, 峯村 采花, 三浦 大輝, 江口 修平, 岡 健太郎, 高橋 志達 and Fumiki Morimatsu :
グアガムの飼料添加によるブタ腸内細菌叢変化の経時的解析,
日本食物繊維学会第26回学術集会, Nov. 2021.
10. Maki Hirata, Miki Matsuoka, 橋本 託真, 小浦 考修, 大貫 燿, 吉田 知加, 峯村 采花, 三浦 大輝, 岡 健太郎, 高橋 志達 and Fumiki Morimatsu :
肥育期ブタへの Clostridium butyricum MIYAIRI 588 給与が腸内細菌叢構成に及ぼす影響,
第71回関西畜産学会, Oct. 2021.
11. Lin Qingyi, Chommanart Thongkittidilok, Maki Hirata, QUYNH ANH LE, K. Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Lipofection-mediated introduction of CRISPR/Cas9 system into porcine zygotes.,
第114回日本繁殖生物学会, Sep. 2021.
12. TAKEBAYASHI Kohki, Chommanart Thongkittidilok, 林 青怡, LE Anh Quynh, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
ノックアウトブタ由来精子を用いた体外受精胚におけるゲノム編集効率の検討,
第114回日本繁殖生物学会, Sep. 2021.
13. Maki Hirata :
畜産領域におけるアニマルウェルフェアとOne health,
第23回臨床腸内微生物学会総会・学術集会, Sep. 2020.
14. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製,
第162回日本獣医学会学術集会, Sep. 2019.
15. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Namula Zhao and Takeshige Otoi :
ブタ体外受精卵におけるCrispr/Cas9システムを使用したゲノム編集の効率,
日本ゲノム編集学会 第3回大会, Jun. 2018.
16. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano and Takeshige Otoi :
ブタ内在性レトロウイルス遺伝子を標的としたゲノム編集が胚発育能に及ぼす影響,
日本ゲノム編集学会第3回大会, Jun. 2018.

Et cetera, Workshop:

1. Chika Yoshida, Maki Hirata, Miki Matuoka, Yoh Ohnuki, Kentaro Oka, Motomichi Takahashi, Masanobu Haraguchi and Fumiki Morimatsu :
豚の飼育施設における有彩色LED照明の利用可能性に関する研究,
LED総合フォーラム2022 in 徳島, P-19, Jan. 2022.
2. Fuminori Tanihara, Maki Hirata, Takeshige Otoi, 澤本 修 and 菊地 健志 :
GEEP法によるGGTA1遺伝子およびCMAH遺伝子の同時改変ブタの作出,
第24回日本異種移植研究会, Feb. 2021.
3. Chika Yoshida, Maki Hirata, Miki Matuoka, Yoh Ohnuki, Kentaro Oka, Motomichi Takahashi, Masanobu Haraguchi and Fumiki Morimatsu :
照明色の違いが豚の攻撃的行動と生産性に及ぼす影響,
LED総合フォーラム2021in徳島, P-19, Feb. 2021.
4. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
CRISPR/Cas9システムによるブタ体外受精卵のINS遺伝子への点変異導入,
第7回日本先進医工学ブタ研究会, Oct. 2019.
5. Takeshige Otoi, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Fuminori Tanihara :
GEEP法を用いた遺伝子改変ブタの作製と遺伝子改変効率,
第7回日本先進医工学ブタ研究会, Oct. 2019.
6. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
ブタ体外受精卵におけるCRISPR/Cas9システムを用いた複数遺伝子の同時改変,
第7回日本先進医工学ブタ研究会, Oct. 2019.
7. Le Anh Quynh, Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano and Takeshige Otoi :
Concentration of CRISPR/Cas9 components effects on genetic mosaicism of cytoplasmic microinjected porcine embryos,
第6回日本先進医工学ブタ研究会, Oct. 2018.
8. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Le Anh Quynh, 新居 雅宏 and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid,
第6回日本先進医工学ブタ研究会, Oct. 2018.
9. Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, 新居 雅宏 and Takeshige Otoi :
ブタにおける受精前後でのゲノム編集が胚盤胞の変異導入効率に及ぼす影響,
第6回日本先進医工学ブタ研究会, Oct. 2018.
10. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Takayuki Hirano, Tatsuya Takemoto, 中井 美智子, 淵本 大一郎 and Takeshige Otoi :
ゲノム編集によるTP53遺伝子改変ブタの作製と表現型の解析,
第6回日本先進医工学ブタ研究会, Oct. 2018.
11. Fuminori Tanihara, Tatsuya Takemoto, Maki Hirata, N Nguyen Thi, Toshiki Kunihara, R Nishinakamura and Takeshige Otoi :
Modification of SALL1 gene via CRISPR/Cas9-mediated gene editing introduced into porcine zygotes by electroporation,
KEY Forum: The 3rd International symposium on Stem Cell Traits and Developmental Systems, Jan. 2018.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

  • Development of animal model for kidney-specific spontaneous tumours (Project/Area Number: 22K19896 )
  • Production of virus infection-resistant animals by a simple genome editing procedure (Project/Area Number: 22H02499 )
  • Production of immunodeficient micro-mini pig using genome editing technology. (Project/Area Number: 18K12062 )
  • The role of p53 during airway and lung injury, repair and regeneration using TP53-mutant porcine (Project/Area Number: 18K08785 )
  • Search by Researcher Number (10815583)