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Tokushima University > Graduate School of Technology, Industrial and Social Sciences > Division of Bioscience and Bioindustry > Agroscience > 生物資源生産科学分野 >
Tokushima University > Research Clusters > Research Clusters (Selected) > 1903008 植物の生老病死を制御する代謝機構の分子・工学的解明とゲノム編集による改変 >
Tokushima University > Research Clusters > Research Clusters (Registered) > 1904009 有用動物細胞株樹立のための新規ゲノム編集ツール高効率導入法の開発 >
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Research Activity

Field of Study

Subject of Study

Book / Paper

Book:

1. Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui and Mitsuo Oshimura :
Production of a Human Cell line with a Plant Chromosome.,
Humana Press, 2018.
(DOI: 10.1007/978-1-4939-7795-6_16,   Elsevier: Scopus)
2. Naoki Wada, Naruemon Khemkladngoen, Joyce A Cartagena and Kiichi Fukui :
Bioactive beads-mediated transformation of plants with large DNA fragments.,
Humana Press, 2012.
3. Khemkladngoen Naruemon, Naoki Wada, Suguru Tsuchimoro, Joyce A Cartagene, Shin'ichiro Kajiyama and Kiichi Fukui :
Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes, with special reference to bead-production methodology.,
InTech, USA, 2012.
4. Naoki Wada, Shin'ichiro Kajiyama, Naruemon Khemkladngoen and Kiichi Fukui :
A novel gene delivery system in plants with calcium alginate micro-beads.,
Wiley-Blackwell Publishers, 2011.

Academic Paper (Judged Full Paper):

1. Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, HIroe Sakai, Naoki Wada, Arefeh Alipour, Tomohiro Sasai, Atsushi Tunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, HIdeaki Hirakawa, Victor Pecina-Quintero, Alfredo Zamarripa and Kiichi Fukui :
Association Study of Seed-Yield Related Traits for Jatropha curcas L. in Mexico,
Tropical Agriculture and Development, Vol.62, No.2, 68-77, 2018.
(DOI: 10.11248/jsta.62.68)
2. Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, Hideki Hirakawa, P Victor Quintero, Alfredo Zamarripa, Primitivo Santos, Adel Hegazy, M Abdalla Ali and Kiichi Fukui :
Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World.,
Frontiers in Plant Science, Vol.8, No.1539, 2017.
(DOI: 10.3389/fpls.2017.01539,   PubMed: 28936216)
3. Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukuui :
Agrobacterium-Mediated Transformation of Jojoba [Simmondsia chinensis (Link) Schneider],
Tropical Agriculture and Development, Vol.61, No.1, 23-31, 2017.
(DOI: 10.11248/jsta.61.23)
4. Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui :
High-frequency Shoots Regeneration of an Oil Crop, Simmondsia chinensis (Link) Schneider Using Axillary Buds.,
Tropical Agriculture and Development, Vol.61, No.1, 15-22, 2017.
(DOI: 10.11248/jsta.61.15)
5. Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui and Mitsuo Oshimura :
Maintenance and Function of a Plant Chromosome in Human Cells.,
ACS Synthetic Biology, Vol.6, No.2, 301-310, 2016.
(DOI: 10.1021/acssynbio.6b00180,   PubMed: 27696824)
6. Misa Fujiwara, Go Suzuki, Daiki Kudo, Haruna Oba, Yukio Wada, Hideo Wada, Naoki Wada, Sadequr Rahman, Kiichi Fukui and Yasuhiko Mukai :
Localization of transgene-derived friabilins in rice endosperm cells,
Plant Biotechnology, Vol.31, No.1, 67-70, 2014.
(DOI: 10.5511/plantbiotechnology.13.1028a)
7. Suguru Tsuchimoto, Joyce A Cartagena, Khemkladngoen Naruemon, Singkaravanit Suthitar, Tsutomu Kohinata, Naoki Wada, HIroe Sakai, Daisuke Shibata and Kiichi Fukui :
Development of transgenic plants in jatropha with drought tolerance,
Plant Biotechnology, Vol.29, No.2, 137-143, 2012.
(DOI: 10.5511/plantbiotechnology.12.0406d)
8. Shusei Sato, Hideki Hirakawa, Sachiko Isobe, Eigo Fukai, Akiko Watanabe, Midori Kato, Kumiko Kawashima, Chiharu Minami, Akiko Muraki, Naomi Nakazaki, Chika Takahashi, Shinobu Nakayama, Yoshie Kishida, Mitsuyo Kohara, Manabu Yamada, Hisano Tsuruoka, Shigemi Sasamoto, Satoshi Tabata, Tomoyuki Aizu, Atsushi Toyoda, Tadasu Shin-i, Yohei Minakuchi, Yuji Kohara, Asao Fujiyama, Suguru Tsuchimoto, Shin'ichiro Kajiyama, Eri Makigano, Nobuko Ohmido, Nakako Shibagaki, A Joyce Cartagena, Naoki Wada, Tsutomu Kohinata, Alipour Atefeh, Shota Yuasa, Sachihiro Matsunaga and Kiichi Fukui :
Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.,
DNA Research, Vol.18, No.1, 65-76, 2010.
(DOI: 10.1093/dnares/dsq030,   PubMed: 21149391)
9. Naoki Wada, Shin'ichiro Kajiyama, Joyce A Cartagena, Linyen Lin, Yukio Akiyama, Motoyasu Otani, Go Suzuki, Yasuhiko Mukai, Noriaki Aoki and Kiichi Fukui :
The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant,
Journal of Cereal Science, Vol.51, No.2, 182-188, 2010.
(DOI: 10.1016/j.jcs.2009.11.010)
10. Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Daisuke No, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes.,
Plant Cell Reports, Vol.28, No.5, 759-768, 2009.
(DOI: 10.1007/s00299-009-0678-2,   PubMed: 19214515)

Review, Commentary:

1. Naoki Wada :
ヒトと植物細胞の細胞融合:16億年の時を超えた再会,
現代化学, No.5, 41-43, 2017.
2. Naoki Wada :
不毛な大地で油を作る植物たち,
生物工学会誌, Vol.93, No.3, 156, 2015.

Proceeding of International Conference:

1. Naoki Wada, Murakami Emi, Hashimoto Ryosuke, Yuriko Osakabe and Keishi Osakabe :
DEVELOPMENT OF A NOVEL GENOME EDITING TOOL, TID SYSTEM, FOR MAMMALIAN GENOME ENGINEERING,
Frontiers in Genome Engineering 2019, Kobe Convention Center, Nov. 25-27, 2019, Nov. 2019.
2. Naoki Wada :
Maintenance and function of a plant chromosome in human cells,
The 11th International Symposium Exploring the Global Sustainability, Mar. 2018.
3. Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui :
Developing industrial oil-seed trees for drylands,
The 10th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
4. Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Hiroe Sakai, Naoki Wada and Kiichi Fukui :
Genetic diversity and the seed-trait variation in jojoba accessions from America, Africa and Asia,
Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
5. Naoki Wada, Yutaka Minote, Hiroe Sakai, Suguru Tsuchimoto, Shin"ichiro Kajiyama and Kiichi Fukui :
Development of in planta liquid wax ester production system by targeting the biosynthetic enzymes into different organelles,
The 10th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
6. Naoki Wada :
A partial hybrid cell line between human and plants,
Interdisciplinary German-Japanese symposium iJaDe 2016 in IPK Gatersleben, May 2016.
7. Naoki Wada :
A partial hybrid cell line between human and plants,
Interdisciplinary German-Japanese symposium iJaDe 2016 in Technische Universität Dresden, May 2016.
8. Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Hisashi Tomemori, Satoshi Tabata, Shusei Sato, Hideki Hirakawa, Victor P Quintero, Alfredo Zamarripa and Kiichi Fukui :
Association analysis of yield-related traits in Jatropha curcas L,
The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
9. Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui :
An efficient in vitro regeneration and transformation system of Jatropha curcas L.,
The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
10. Chiaki Katsuura, Naoki Wada, Hiroe Sakai, Suguru Tsuchimoto and Kiichi Fukui :
Screening and identification of effective genes for reinforcing drought tolerance of Jatropha curcas L,
The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2016.
11. Suguru Tsuchimoto, Ismail A Mohammed, Hayiyan Li, Minote Yutaka, Kyuya Harada, Hiroe Sakai, Naoki Wada, Adel Hegazy, Tsutomu Fushimi and Kiichi Fukui :
Jojoba: Research and practice,
The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
12. Naoki Wada :
A hybrid cell line between human and plant,
The 2nd UK-Japan Chromosome Workshop, May 2015.
13. Naoki Wada, Yasuhiro Kazuki, Kiichi Fukui and MItsuo Oshimura :
A hybrid cell line between human and plant,
The 5th Asian Chromosome Colloquium, May 2015.
14. Naoki Wada :
Application of plant biotechnology to Shimmondsia chinesis (jojoba) plants,
The 8th International Symposium Exploring the global sustainability Advances in Plant Biotechnology for Agriculture in Semi-arid land, Mar. 2015.
15. Naoki Wada :
Importance of jojoba (Shimondsia chinensis) as a strategic bioresource,
Genetic Engineering and Biotechnology Research Institute (GEBRI) and Tissue Culture and Genetic Engineering Center Seminar in University of Sadat City, Egypt, Oct. 2014.
16. Suguru Tsuchimoro, Joyce A Cartagena, Naruemon Khemkladngoen, Suithitar Singkaravanit, Tsutomu Kohinata, Naoki Wada, Hiroe Sakai, Yoshihiko Morishita, Hideyuki Suzuki, Daisuke Shibata and Kiichi Fukui :
DEVELOPMENT OF JATROPHA TRANSGENIC PLANTS WITH DROUGHT TOLERANCE,
International Conference for Bioenergy and Biomass Utilization, Aug. 2012.
17. Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Joyce A Cartagena, Linyen Lin, Noriaki Aoki, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii gene,
Japanese-German JSPS and DFG-funded workshop: Frontiers of Plant Chromosome Research: Centromeres and Artificial Chromosomes, Nov. 2011.
18. Naoki Wada, Joyce A Cartagena, Naruemon Khemkladngoen, Tsutomu Kohinata and Kiichi Fukui :
Transformation of Jatropha curcas with Phosphopantetheine Adenylyltrasnferaes (PPAT) gene to enhance the drought stress tolerance,
Symposium on Jatropha Research: Updates and Future Perspectives, Jan. 2011.
19. Naoki Wada :
油糧植物ジャトロファからの環境問題へのアプローチ,
中日学者草原生態問題学術座談会 in 内モンゴル自治区社会科学研究院, Aug. 2010.
20. Naoki Wada, Shin'ichiro Kajiyama, Joyce A Cartagena, Yukio AKiyama, Lin Linyen, Noriaki Aoki, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Bioactive Beads-Mediated Transformation Of Rice With Large DNA Fragments Containing Aegilops tauschii Hardness Genes,
Plant and Animal Genome XVIII Conference, Feb. 2010.
21. Kiichi Fukui, Naoki Wada and Susumu Uchiyama :
Bioactive beads-mediated transformation of plants with large DNA fragments,
Plant and Animal Genome XX Conference, Jan. 2010.
22. Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Transformation of rice with large DNA fr agments using a bio-active beads metho,
8th Osaka University-KAIST Graduate Students Symposium in Biotechnology, Feb. 2009.
23. Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Transformation of rice with high molecular weight DNAs using bio-active beads method,
The 3rd Asian Chromosome Colloquium, Dec. 2008.
24. Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui :
Development of a New Plant Transformation System with Large DNAs using Bioactive Beads Method,
The 1st international global COE symposium on Bio-Enviromental Chemistry, Jan. 2008.
25. Kiichi Fukui, Naoki Wada, Naruemon Khemkladngoen and Shin'ichiro Kajiyama :
A novel gene delivery system in plants with calcium alginate micro-beads,
Plant Transformation technologies, Feb. 2007.
26. Naoki Wada, Koichi Ito, Naoko Nose, Yukio Akiyama, Shigeki Kawakami, Sachihiro Matsunaga, Motoyasu Otani, Go Suzuki, Yasuhiko Mukai, Takiko Shimada and Kiichi Fukui :
Transformation of Rice Plants Using Bio-Active Beads with BACs,
Plant & Animal Genome XIV, Jan. 2006.

Proceeding of Domestic Conference:

1. Ueta Risa, Kira Nozomu, Yoshioka Rika, Miyaji Tomoko, Naoki Wada, Yuriko Osakabe and Keishi Osakabe :
CRISPR/dCas9を利用した植物遺伝子発現制御システムの開発,
第37回日本植物細胞分子生物学会, Sep. 2019.
2. Naoki Wada, Murakami Emi, Yuriko Osakabe and Keishi Osakabe :
Development of a highly sensitive guide RNA evaluation system using Nano Luciferase,
The 12th International Symposium Exploring the Global Sustainability, Kindai University, 201985, Aug. 2019.
3. Naoki Wada, 村上 愛美, Yuriko Osakabe and Keishi Osakabe :
Nano Luciferaseを用いた高感度ガイドRNA評価システムの開発,
日本ゲノム編集学会第4回大会, Jun. 2019.
4. Risa Ueta, 宮地 朋子, Naoki Wada, Yuriko Osakabe and Keishi Osakabe :
Control of plant gene expression by using CRISPR/dCas9 system,
第60回日本植物生理学会年会, Mar. 2019.
5. Matsunaga Sachihiro, Ishiyama Sumire, Naoki Wada and Matsunaga Tomoko :
Construction of hybrid cells between algae and human cultured cells toward creation of planimal cells,
第30回日本植物形態学会, Sep. 2018.
6. Naoki Wada, 香月 康宏, 香月 加奈子, 井上 敏昭, Keishi Osakabe, 福井 希一 and 押村 光雄 :
植物/ヒト雑種細胞における植物染色体の挙動と遺伝子発現,
第59回日本植物生理学会, Mar. 2018.
7. Naoki Wada :
ヒトと植物細胞の部分的な融合細胞株の樹立 (招待講演),
第38回日本動物細胞工学会シンポジウム, Mar. 2018.
8. Naoki Wada :
ヒトと植物細胞の部分的な融合細胞株の樹立—進化を超えた染色体機能保存性の解明を目指してー (招待講演),
第5回細胞凝集研究会, Nov. 2017.
9. Naoki Wada, 香月 康宏, 香月 加奈子, 井上 敏昭, 福井 希一 and 押村 光雄 :
ヒト細胞における植物染色体の維持と機能,
第39回日本分子生物学会, Dec. 2016.
10. Haiyan Li, 土本 卓, 原田 久也, 山崎 将紀, 酒井 啓江, Naoki Wada, Alipour Atefeh, 笹井 知博, 恒川 篤史, 辻本 壽, 安藤 孝之, 留森 寿士, 佐藤 修正, 平川 英樹, Wuintero Pecina Victor, Zamarripa Alfredo, Santos Primitivo, Hegazy Adel, Ali Mohamed Abdalla and 福井 希一 :
DNA マーカーを用いた燃料作物ジャトロファの起源中心の遺伝学的解析,
第130回日本育種学会, Sep. 2016.
11. Naoki Wada :
乾燥ストレス耐性を付与した油糧ジャトロファの創出,
VBL異分野融合研究会, Nov. 2010.
12. Naoki Wada, 梶山 慎一郎, Joyce A Cartagena, 林 麟晏, 秋山 征夫, 大谷 基泰, 鈴木 剛, 向井 康比己, 青木 法明, 内山 進, 松永 幸大 and 福井 希一 :
バイオアクティブビーズ法を用いた巨大DNA導入による新規形質転換イネの作出,
H20年度先端科学イノベーションセンター公開成果発表会, Mar. 2010.
13. Naoki Wada, 梶山 慎一郎, 秋山 征夫, 大谷 基泰, 島田 多喜子, 鈴木 剛, 向井 康比己 and 福井 希一 :
バイオアクティブビーズ法によるハードネス遺伝子群導入イネの作出,
第114回 日本育種学会, Oct. 2008.
14. Naoki Wada, 池内 智彦, 梶山 慎一郎, 大谷 基泰, 鈴木 剛, 向井 康比己, 島田 多喜子 and 福井 希一 :
バイオアクティブビーズ法によるコムギ有用遺伝子群を含む巨大DNAのイネへの導入,
大阪大学イノベーションセミナー2007, Oct. 2007.
15. Naoki Wada, 秋山 征夫, 大谷 基泰, 鈴木 剛, 向井 康比己, 島田 多喜子 and 福井 希一 :
バイオアクティブビーズ法によるコムギ有用遺伝子群を含む巨大DNAのイネへの導入,
第110回日本育種学会, Sep. 2006.
16. Naoki Wada, 秋山 征夫, 大谷 基泰, 鈴木 剛, 向井 康比己, 島田 多喜子 and 福井 希一 :
バイオアクティブビーズ法を用いた巨大遺伝子群の導入による新型有用米の開発,
VBL公開成果発表会, Mar. 2006.
17. Naoki Wada, 川上 茂樹, 松永 幸大, 向井 康比己, 島田 多喜子 and 福井 希一 :
バイオアクティブビーズ法を用いた新規植物「パンコメ」の開発,
第57回日本生物工学会, Nov. 2005.
18. Naoki Wada, 川上 茂樹, 松永 幸大, 向井 廉比己, 島田 多喜子 and 福井 希一 :
バイオアクティブビーズ法を用いた新規植物「パンコメ」の開発,
大阪大学イノベーションセミナー2005, Oct. 2005.
19. Naoki Wada and 福井 希一 :
バイオアクティブビーズ法を用いた新型食品の開発,
第5回阪大フロンティア・シンポジウム・2004年度研究成果展示発表会, May 2005.
20. Naoki Wada, 川上 茂樹, 松永 幸大, 内山 進 and 福井 希一 :
バイオビーズ法を用いた新規植物"パンコメ"の作出,
2004年度先端科学イノベーションセンターVBL部門公開成果発表会, Mar. 2005.
21. Naoki Wada, 川上 茂樹, 向井 康比己 and 福井 希一 :
バイオビーズ法を用いた新規植物「パンコメ」の作出,
近畿地域アグリビジネス創出フェア, Dec. 2004.
22. Naoki Wada, 川上 茂樹, 向井 廉比己 and 福井 希一 :
バイオビーズ法を用いた新規植物「パンコメ」の作出,
バイオメディカルベンチャー創出を目指した若手交流会, Oct. 2004.
23. Naoki Wada, 松永 幸大, 内山 進 and 福井 希一 :
バイオアクティブビーズを用いた遺伝子デリバリー,
第一回イノベーション2004, Oct. 2004.
24. Naoki Wada and 福井 希一 :
遺伝子改変による新型食品の開発,
イノベーション・ジャパン2004, Sep. 2004.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)