Tokushima University / Educator and Researcher Directory --- Umeshima, Hiroki

Personal Web Page

https://researchmap.jp/7000010705 (researchmap)

Field of Study

○	Life sciences [Neuroscience - general]

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1.	Teruyoshi Hirayama, Y Kadooka, E Tarusawa, S Saitoh, H Nakayama, N Hoshino, S Nakama, T Fukuishi, Y Kawanishi, Hiroki Umeshima, Koichi Tomita, Y Yoshimura, N Galjart, K Hashimoto, N Ohno and T Yagi : CTCF loss induces giant lamellar bodies in Purkinjecell dendrites., Acta Neuropathologica Communications, Vol.10, No.1, 172, 2022. (Summary) CCCTC-binding factor (CTCF) has a key role in higher-order chromatin architecture that is important for establishing and maintaining cell identity by controlling gene expression. In the mature cerebellum, CTCF is highly expressed in Purkinje cells (PCs) as compared with other cerebellar neurons. The cerebellum plays an important role in motor function by regulating PCs, which are the sole output neurons, and defects in PCs cause motor dysfunction. However, the role of CTCF in PCs has not yet been explored. Here we found that the absence of CTCF in mouse PCs led to progressive motor dysfunction and abnormal dendritic morphology in those cells, which included dendritic self-avoidance defects and a proximal shift in the climbing fibre innervation territory on PC dendrites. Furthermore, we found the peculiar lamellar structures known as "giant lamellar bodies" (GLBs), which have been reported in PCs of patients with Werdnig-Hoffman disease, 13q deletion syndrome, and Krabbe disease. GLBs are localized to PC dendrites and are assumed to be associated with neurodegeneration. They have been noted, however, only in case reports following autopsy, and reports of their existence have been very limited. Here we show that GLBs were reproducibly formed in PC dendrites of a mouse model in which CTCF was deleted. GLBs were not noted in PC dendrites at infancy but instead developed over time. In conjunction with GLB development in PC dendrites, the endoplasmic reticulum was almost absent around the nuclei, the mitochondria were markedly swollen and their cristae had decreased drastically, and almost all PCs eventually disappeared as severe motor deficits manifested. Our results revealed the important role of CTCF during normal development and in maintaining PCs and provide new insights into the molecular mechanism of GLB formation during neurodegenerative disease. (Keyword) Animals / Mice / Purkinje Cells / Lamellar Bodies / Neurodegenerative Diseases / Cerebellum / Dendrites (Link to Publication Site) ● Publication site (DOI): 10.1186/s40478-022-01478-6 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36447271 ● Search Scopus @ Elsevier (PMID): 36447271 ● Search Scopus @ Elsevier (DOI): 10.1186/s40478-022-01478-6 (DOI: 10.1186/s40478-022-01478-6, PubMed: 36447271)

Review, Commentary:

1.	Hiroki Umeshima : 牽引力顕微鏡法を用いた神経細胞の核移動に伴う牽引力の検出, Neuroscience News, No.2019 NO.1, 27, Feb. 2019.

Proceeding of Domestic Conference:

1.	Koichi Tsuneyama, Teruyoshi Hirayama, Hiroki Umeshima and Koichi Tomita : 系統解剖実習を活用した解剖・病理垂直連携の試み, 129, Mar. 2024.
2.	Koichi Tomita, Teruyoshi Hirayama, Hiroki Umeshima, Koichi Tsuneyama, Akiyoshi Nishimura, Hideyuki Nushida, Masashi Akaike, Hiromitsu Takizawa, Mitsuo Shimada, Yasushi Takagi, Ichiro Hashimoto and Takashi Iwata : 徳島大学医学部の系統解剖実習における画像診断技術・病理診断技術・外科的手術手技を取り入れた垂直連携教育の実践, 第129回日本解剖学会全国学術集会, Mar. 2024.
3.	Teruyoshi Hirayama, 角岡佑紀, 足澤悦子, 齋藤成, 中山寿子, 星野七海, 仲間宗一郎, 福石高弘, 川西雄大, Hiroki Umeshima, Koichi Tomita, 吉村由美子, Galjart Niels, 橋本浩一, 大野伸彦 and 八木健 : CTCF, 第128回日本解剖学会全国学術集会, Mar. 2023.
4.	Hiroki Umeshima, Teruyoshi Hirayama and Koichi Tomita : マウス大脳皮質におけるHeat Shock Protein 90のアイソフォーム特異的発現パターンの解析, 第128回日本解剖学会全国学術集会, Mar. 2023.
5.	Hiroki Umeshima : 脳の発生過程における神経細胞移動を駆動する力学メカニズムの解析, 第261回徳島医学会学術集会, Aug. 2020.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Effects of RNA modification and local translation in the growth cone on neural circuit formation (Project/Area Number: 18K06464 )
ニューロン遊走における核レオロジー解析と脳皮質3次元細胞構築への展開 (Project/Area Number: 26106715 )
Analysis of nuclear and cytoskeletal dynamics underlying neuronal migration (Project/Area Number: 26830012 )
analysis of molecular mechanisms underlying nuclear translocation in migrating neurons (Project/Area Number: 22700337 )
Search by Researcher Number (40525375)

Assistant Professor : Umeshima, Hiroki

Research

Personal Web Page

Field of Study

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

Review, Commentary:

Proceeding of Domestic Conference:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)