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Tokushima UniversityInstitute of Advanced Medical Sciences基幹研究部門 (Files for researchmap) [PDF manual] [Auto-propagate to researchmap]

Research Activity

Field of Study

Biophysics

Subject of Study

Unraveling molecular mechanism of cellular homeostasis

Book / Paper

Academic Paper (Judged Full Paper):

1. Motonori Matsusaki, Okada Rina, Tanikawa Yuya, Shingo Kanemura, Ito Dai, Lin Yuxi, Watabe Mai, Yamaguchi Hiroshi, Tomohide Saio, Lee Young-Ho, Inaba Kenji and Okumura Masaki :
Functional Interplay between P5 and PDI/ERp72 to Drive Protein Folding,
biology, Vol.10, No.11, 1112, 2021.
(Tokushima University Institutional Repository: 116440)
2. Hiroshi Nakagawa, Tomohide Saio, Michihiro Nagao, Rintaro Inoue, Masaaki Sugiyama, Satoshi Ajito, Taiki Tominaga and Yukinobu Kawakita :
Conformational dynamics of a multidomain protein by neutron scattering and computational analysis.,
Biophysical Journal, Vol.120, No.16, 3341-3354, 2021.
(DOI: 10.1016/j.bpj.2021.07.001,   PubMed: 34242590)
3. Tomohide Saio, Soya Hiramatsu, Mizue Asada, Hiroshi Nakagawa, Kazumi Shimizu, Hiroyuki Kumeta, Toshikazu Nakamura and Koichiro Ishimori :
electron paramagnetic resonance.,
Biophysical Journal, Vol.120, No.15, 2943-2951, 2021.
(DOI: 10.1016/j.bpj.2021.06.033,   PubMed: 34242587)
4. Masahiro Mimura, Shunsuke Tomita, Yoichi Shinkai, Takuya Hosokai, Hiroyuki Kumeta, Tomohide Saio, Kentaro Shiraki and Ryoji Kurita :
Quadruplex Folding Promotes the Condensation of Linker Histones and DNAs via Liquid-Liquid Phase Separation.,
Journal of the American Chemical Society, Vol.143, No.26, 9849-9857, 2021.
(DOI: 10.1021/jacs.1c03447,   PubMed: 34152774)
5. Masaki Okumura, Shingo Kanemura, Motonori Matsusaki, Misaki Kinoshita, Tomohide Saio, Dai Ito, Chihiro Hirayama, Hiroyuki Kumeta, Mai Watabe, Yuta Amagai, Young-Ho Lee, Shuji Akiyama and Kenji Inaba :
A unique leucine-valine adhesive motif supports structure and function of protein disulfide isomerase P5 via dimerization.,
Structure, 2021.
(DOI: 10.1016/j.str.2021.03.016,   PubMed: 33857433)
6. Aya Okuda, Rintaro Inoue, Ken Morishima, Tomohide Saio, Yasuhiro Yunoki, Maho Yagi-Utsumi, Hirokazu Yagi, Masahiro Shimizu, Nobuhiro Sato, Reiko Urade, Koichi Kato and Masaaki Sugiyama :
Deuteration Aiming for Neutron Scattering.,
Biophysics and Physicobiology, Vol.18, 16-27, 2021.
(DOI: 10.2142/biophysico.bppb-v18.003,   PubMed: 33954079)
7. Kamran Rizzolo, Hsiung Angela Yeou Yu, Adedeji Ologbenla, Rang Sa Kim, Haojie Zhu, Koichiro Ishimori, Guillaume Thibault, Elisa Leung, Wen Yi Zhang, Mona Teng, Marta Haniszewski, Noha Miah, Sadhna Phanse, Zoran Minic, Sukyeong Lee, Diaz Julio Caballero, Mohan Babu, F Francis T Tsai, Tomohide Saio and A Walid Houry :
Functional cooperativity between the trigger factor chaperone and the ClpXP proteolytic complex.,
Nature Communications, Vol.12, No.1, 2021.
(DOI: 10.1038/s41467-020-20553-x,   PubMed: 33436616)
8. Rintaro Inoue, Takashi Oda, Hiroshi Nakagawa, Taiki Tominaga, Tomohide Saio, Yukinobu Kawakita, Masahiro Shimizu, Aya Okuda, Ken Morishima, Nobuhiro Sato, Reiko Urade, Mamoru Sato and Masaaki Sugiyama :
Dynamics of proteins with different molecular structures under solution condition.,
Scientific Reports, Vol.10, No.1, 2020.
(DOI: 10.1038/s41598-020-78311-4,   PubMed: 33303822)
9. Yuya Taguchi, Tomohide Saio and Daisuke Kohda :
Distance Distribution between Two Iodine Atoms Derived from Small-Angle X-ray Scattering Interferometry for Analyzing a Conformational Ensemble of Heavy Atom-Labeled Small Molecules.,
The Journal of Physical Chemistry Letters, Vol.11, No.14, 5451-5456, 2020.
(DOI: 10.1021/acs.jpclett.0c01107,   PubMed: 32558579)
10. Masaki Okumura, Kentaro Noi, Shingo Kanemura, Misaki Kinoshita, Tomohide Saio, Yuichi Inoue, Takaaki Hikima, Shuji Akiyama, Teru Ogura and Kenji Inaba :
Dynamic assembly of protein disulfide isomerase in catalysis of oxidative folding.,
Nature Chemical Biology, Vol.15, No.5, 499-509, 2019.
(DOI: 10.1038/s41589-019-0268-8,   PubMed: 30992562)
11. Soichiro Kawagoe, Hiroshi Nakagawa, Hiroyuki Kumeta, Koichiro Ishimori and Tomohide Saio :
isomerization of unfolded proteins catalyzed by the trigger factor chaperone.,
The Journal of Biological Chemistry, Vol.293, No.39, 15095-15106, 2018.
(DOI: 10.1074/jbc.RA118.003579,   PubMed: 30093407)
12. Tomohide Saio, Soichiro Kawagoe, Koichiro Ishimori and G Charalampos Kalodimos :
Oligomerization of a molecular chaperone modulates its activity.,
eLife, Vol.7, 2018.
(DOI: 10.7554/eLife.35731,   PubMed: 29714686)
13. Wataru Sato, Takeshi Uchida, Tomohide Saio and Koichiro Ishimori :
Polyethylene glycol promotes autoxidation of cytochrome c.,
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1862, No.6, 1339-1349, 2018.
(DOI: 10.1016/j.bbagen.2018.03.010,   PubMed: 29540304)

Review, Commentary:

1. Kawagoe Soichiro, Mori Eiichiro and Tomohide Saio :
Regulation of Liquid-Liquid Phase Separation by Molecular Chaperones,
Thermal Medicine, Vol.37, No.2, 31-44, Aug. 2021.
(DOI: 10.3191/thermalmed.37.31,   CiNii: 130008071965)
2. Takuya Yoshizawa, Ryu-Suke Nozawa, Z Tony Jia, Tomohide Saio and Eiichiro Mori :
Biological phase separation: cell biology meets biophysics.,
Biophysical Reviews, Vol.12, No.2, 519-539, Mar. 2020.
(DOI: 10.1007/s12551-020-00680-x,   PubMed: 32189162)
3. Masaaki Sugiyama, Rintaro Inoue, Hiroshi Nakagawa and Tomohide Saio :
Solution Neutron Scattering,
hamon, Vol.30, No.1, 16-25, 2020.
(DOI: 10.5611/hamon.30.1_16,   CiNii: 130008047185)
4. 小田 隆 and Tomohide Saio :
検出技術 : 天然変性タンパク質の構造生物学 (特集 相分離生物学 : 相分離メガネのススメ),
Seibutsu-kogaku Kaishi, Vol.98, No.5, 232-235, 2020.
(CiNii: 40022261152)
5. Tomohide Saio and Koichiro Ishimori :
Accelerating structural life science by paramagnetic lanthanide probe methods.,
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1864, No.2, 129332, Mar. 2019.
(DOI: 10.1016/j.bbagen.2019.03.018,   PubMed: 30928492)

Proceeding of International Conference:

1. Tomohide Saio :
Conformational Variation of a Multi-Domain Protein Enzyme Investigated by Paramagnetic Lanthanide Probe,
ISMAR-APNMR2021, Aug. 2021.
2. Tomohide Saio, Hiroshi Nakagawa, Soya Hiramatsu, Mizue Asada, Honoka Kawamukai, Toshikazu Nakamura and Koichiro Ishimori :
Application of a lanthanide tag for evaluation of conformational states of a multidomain protein,
IUCr 2021 - XXV General Assembly and Congress of the International Union of Crystallography, Aug. 2021.
3. Tomohide Saio :
Mechanistic insights into a molecular chaperone in protein folding and degradation,
2021 virtual Cold Spring Harbor Asia Conference, Mar. 2021.
4. Tomohide Saio :
On and off between molecular chaperones and clients: Appropriate distance and timing for protein folding,
21st Hokudai-RIES International Symposium, Dec. 2020.

Proceeding of Domestic Conference:

1. Tomohide Saio :
常磁性金属と分子シャペロン,
2020年度 日本分光学会NMR分光部会 集中講義, Oct. 2020.
2. Tomohide Saio and 石森 浩一郎 :
Exploiting paramagnetic metal ions for protein structural study in solution,
The 58th Annual Meeting of the BSJ, Sep. 2020.
3. Tomohide Saio :
Understanding molecular mechanism of chaperone-mediated proteostasis in the cell,
第93回日本生化学会大会, Sep. 2020.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

  • 生体分子の構造形成における遅延制御メカニズム解明 (Project/Area Number: 21H05094 )
  • Structural basis for recognition and regulation of protein folding intermediate by molecular chaperones in the endoplasmic reticulum (Project/Area Number: 20KK0156 )
  • Regulatory mechanism of biological phase separation (Project/Area Number: 20H03199 )
  • 相分離を介したシグナル伝達制御の構造基盤 (Project/Area Number: 19H04945 )
  • 半特異的認識を介したシャペロンネットワークの構造基盤 (Project/Area Number: 19K06504 )
  • 新世代中性子構造生物学の開拓 (Project/Area Number: 18H05229 )
  • NMRを主体としたタンパク質構造推移解析のための複合手法の開発と応用 (Project/Area Number: 17H05867 )
  • 分子シャペロンの基質選択と活性発現における動的構造基盤 (Project/Area Number: 17H05657 )
  • Dynamic Structural Characterization of Electron Transfer Complex in Respiratory Chain of Mitochondria and Its Electron Transfer Regulation Mechanism (Project/Area Number: 16H04173 )
  • NMRを主体としたタンパク質構造推移解析のための複合手法の開発と応用 (Project/Area Number: 15H01624 )
  • Analysis of respiratory electron transport chain using the paramagnetic probes (Project/Area Number: 15K20829 )
  • Functional and Structural Characterization of Electron Transfer Reactions in Mitochondria Respiratory Chain Utilizing Nanodisc (Project/Area Number: 25288072 )
  • Search by Researcher Number (80740802)