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Tokushima UniversityGraduate School of Technology, Industrial and Social SciencesDivision of Bioscience and BioindustryAgroscienceBioresource Science and Economics
Tokushima UniversityBio-Innovation Research Center
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Research

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Field of Study

Life sciences [Animal production science]
Life sciences [Veterinary medicine]

Subject of Study

Book / Paper

Academic Paper (Judged Full Paper):

1. Koki Takebayashi, Manita Wittayarat, Maki Hirata, Qingyi Lin, Zhao Namula, Nanaka Torigoe, Bin Liu, Megumi Nagahara, Aya Nakai, Takeshige Otoi and Fuminori Tanihara :
Optimization of embryonic stage for aggregation to generate chimeric pigs using gene-edited blastomeres.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00987-z,   PubMed: 39446279)
2. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.,
The Journal of Reproduction and Development, 2024.
(DOI: 10.1262/jrd.2024-054,   PubMed: 39218670)
3. Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Fuminori Tanihara, Takeshige Otoi and Maki Hirata :
Effects of ergothioneine supplementation on meiotic competence and porcine oocyte development.,
Veterinary World, Vol.17, No.8, 1748-1752, 2024.
(DOI: 10.14202/vetworld.2024.1748-1752,   PubMed: 39328438)
4. Bin Liu, Manita Wittayarat, Koki Takebayashi, Qingyi Lin, Nanaka Torigoe, Zhao Namula, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Effects of centrifugation treatment before electroporation on gene editing in pig embryos.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00926-y,   PubMed: 38833208)
5. Thi Suong Nguyen, Masayasu Taniguchi, Tetsushi Ono, Mitsuhiro Takagi, Qingyi Lin, Nanaka Torigoe, Bin Liu, Zhao Namula, Megumi Nagahara and Takeshige Otoi :
Quality and fertilizing ability of frozen-thawed porcine sperm separated using a migration sedimentation method.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.59, No.6, 2024.
(DOI: 10.1111/rda.14648,   PubMed: 38877771)
6. Megumi Nagahara, Satoshi Tatemoto, Takumi Ito, Otoha Fujimoto, Tetsushi Ono, Masayasu Taniguchi, Mitsuhiro Takagi and Takeshige Otoi :
Designing a diagnostic method to predict the optimal artificial insemination timing in cows using artificial intelligence,
Frontiers in Animal Science, Vol.5, No.1399434, 2024.
(DOI: 10.3389/fanim.2024.1399434)
7. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00908-0,   PubMed: 38664280)
8. Thanh-Van Nguyen, Kim Lanh Thi Do, Qingyi Lin, Megumi Nagahara, Zhao Namula, Manita Wittayarat, Maki Hirata, Takeshige Otoi and Fuminori Tanihara :
Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00869-4,   PubMed: 38485817)
9. Thanh-Van Nguyen, Koki Takebayashi, Kim Lanh Thi Do, Zhao Namula, Manita Wittayarat, Megumi Nagahara, Maki Hirata, Takeshige Otoi and Fuminori Tanihara :
Generation of allogenic chimera carrying mutations in PDX1 and TP53 genes via phytohemagglutinin-mediated blastomere aggregation in pigs.,
In Vitro Cellular & Developmental Biology. Animal, 2024.
(DOI: 10.1007/s11626-024-00870-x,   PubMed: 38379097)
10. Supitcha Kaewma, Zhao Namula, Thi Suong Nguyen, Qingyi Lin, Nanaka Torigoe, Bin Liu, Megumi Nagahara, Masahiro Nii, Masayasu Taniguchi and Takeshige Otoi :
Effects of ergothioneine supplementation on the quality of liquid-preserved and frozen-thawed boar semen.,
Acta Veterinaria Hungarica, Vol.71, No.3-4, 219-222, 2024.
(DOI: 10.1556/004.2023.00954,   PubMed: 38193983,   Elsevier: Scopus)
11. T Suong Nguyen, Ayane Edo, Megumi Nagahara, Takeshige Otoi, Masayasu Taniguchi and Mitsuhiro Takagi :
Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device.,
Animal Reproduction Science, Vol.260, No.260, 2024.
(DOI: 10.1016/j.anireprosci.2023.107386,   PubMed: 38056176)
12. Nanaka Torigoe, Megumi Nagahara, Thi Suong Nguyen, Qingyi Lin, Koki Takebayashi, Bin Liu, Mutsumi Aihara, Masayasu Taniguchi and Takeshige Otoi :
Development of porcine embryos cultured in media irradiated with ultraviolet-C.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.59, No.1, e14520, 2024.
(DOI: 10.1111/rda.14520,   PubMed: 38268205)
13. Shogo Hashimoto, Masayasu Taniguchi, Ayane Edo, Tetsushi Ono, Tetty Barunawati Siagian, Hiroaki Sekine, Megumi Nagahara, Takeshige Otoi and Mitsuhiro Takagi :
Impact of redox status of donor cows before superovulation treatment on in vivo embryo production.,
Archives Animal Breeding, Vol.66, No.4, 433-437, 2023.
(DOI: 10.5194/aab-66-433-2023,2023,   Elsevier: Scopus)
14. Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos.,
Animal Science Journal, Vol.94, No.1, e13878, 2023.
(DOI: 10.1111/asj.13878,   PubMed: 37818780)
15. Hisayoshi Omori, Junko Chikamoto, Megumi Nagahara, Maki Hirata and Takeshige Otoi :
Evaluating variations in bilirubin glucuronidation activity by protease inhibitors in canine and human primary hepatocytes cultured in a 3D culture system.,
Toxicology In Vitro, Vol.93, 2023.
(DOI: 10.1016/j.tiv.2023.105689,   PubMed: 37660998)
16. Chommanart Thongkittidilok, Maki Hirata, Qingyi Lin, Nanaka Torigoe, Bin Liu, Yoko Sato, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study.,
Veterinary Medicine International, Vol.2023, 7000858, 2023.
(Tokushima University Institutional Repository: 119494,   DOI: 10.1155/2023/7000858,   PubMed: 37609627,   Elsevier: Scopus)
17. Megumi Nagahara, 南雲 翔子 and 大橋 さやか :
肺炎罹患牛における気管深部洗浄液回収方法の検討,
家畜診療, Vol.70, No.722, 453-460, 2023.
(Tokushima University Institutional Repository: 119567)
18. Fuminori Tanihara, Maki Hirata, Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Qingyi Lin, Koki Takebayashi, Hiromasa Hara, Megumi Nagahara and Takeshige Otoi :
GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system.,
Molecular Biology Reports, Vol.50, No.6, 5049-5057, 2023.
(DOI: 10.1007/s11033-023-08388-3,   PubMed: 37101010)
19. Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Mutsumi Aihara, Masahiro Nii and Takeshige Otoi :
Effects of curcumin supplementation on quality of porcine spermatozoa irradiated with ultraviolet-C at 228 nm during liquid preservation.,
Reproduction in Domestic Animals = Zuchthygiene, 2023.
(DOI: 10.1111/rda.14362,   PubMed: 37081592)
20. Thanh-Van Nguyen, Kim Lanh Thi Do, Zhao Namula, Qingyi Lin, Nanaka Torigoe, Megumi Nagahara, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Development and Genome Mutation of Bovine Zygotes Vitrified Before and After Genome Editing via Electroporation,
Cryo Letters, Vol.44, No.2, 118-122, 2023.
(DOI: 10.54680/fr23210110612,   Elsevier: Scopus)
21. Qingyi Lin, Mutsumi Aihara, Akihiro Shirai, Ami Tanaka, Koki Takebayashi, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Takeo Minamikawa and Takeshige Otoi :
Porcine embryo development and inactivation of microorganisms after ultraviolet-C irradiation at 228 nm,
Theriogenology, Vol.197, 252-258, 2023.
(DOI: 10.1016/j.theriogenology.2022.12.015,   PubMed: 36525864)
22. Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Nanaka Torigoe, Bin Liu, Maki Hirata, Megumi Nagahara, Fuminori Tanihara and Takeshige Otoi :
Efficiency of genetic modification in gene-knockout sperm-derived zygotes followed by electroporation of guide RNA targeting the same gene.,
Animal Science Journal, Vol.94, No.1, e13842, 2023.
(DOI: 10.1111/asj.13842,   PubMed: 37218074,   Elsevier: Scopus)
23. Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Maki Hirata, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Kim Lanh Thi Do, Fuminori Tanihara and Takeshige Otoi :
Gene editing in porcine embryos using a combination of electroporation and transfection methods.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.10, 1136-1142, 2022.
(DOI: 10.1111/rda.14184,   PubMed: 35699358)
24. Megumi Shimazaki, Manita Wittayarat, Rentsenkhand Sambuu, Asami Sugita, Masaki Kawaguchi, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi, Takeshige Otoi and Yoko Sato :
Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle-yaks affects spermatogenic cell fate and sterility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.9, 999-1006, 2022.
(DOI: 10.1111/rda.14166,   PubMed: 35614560,   Elsevier: Scopus)
25. Praopilas Phakdeedindan, Manita Wittayarat, Theerawat Tharasanit, Mongkol Techakumphu, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Takeshige Otoi and Yoko Sato :
Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.57, No.3, 304-313, 2021.
(DOI: 10.1111/rda.14061,   PubMed: 34854139)
26. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi :
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020.
(DOI: 10.1111/rda.13609,   PubMed: 31858644)
27. Megumi Shimazaki, Urasoko Saki, Tanaka Masako, Sato Yoko, Fuminori Tanihara, Maki Hirata, Taniguchi Masayasu, Takagi Mitsuhiro and Takeshige Otoi :
Effects of Orvus Es Paste (OEP) on the viability of bull spermatozoa after double freezing and thawing.,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, 32-38, 2018.
(CiNii: 1010003825435470592,   Elsevier: Scopus)
28. Megumi Shimazaki, Rentsenkhand Sambuu, Yoko Sato, Kim Lanh Thi Do, Fuminori Tanihara, Masayasu Taniguchi and Takeshige Otoi :
EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.,
Cryo Letters, Vol.36, No.4, 264-269, 2015.
(PubMed: 26576001,   Elsevier: Scopus)

Proceeding of International Conference:

1. B Liu, Takeshige Otoi, TAKEBAYASHI Kohki, Wittayarat Manita, Maki Hirata, Q. Lin, N. Torigoe, Megumi Nagahara, Namula Zhao and Fuminori Tanihara :
Trial to generate chimeric pigs with high-frequency renal tumors via aggregation of gene-edited blastomeres.,
27th Annual ESDAR Conference, Sep. 2024.
2. Li Qingyi, K. Takebayashi, N. Torigoe, Liu Bin, Maki Hirata, Fuminori Tanihara, Megumi Nagahara and Takeshige Otoi :
Genome editing of porcine zygotes through the lipofection of a CRISPR/Cas9 system with two guide RNAs.,
The 50th Conference of the International Embryo Technology Society, Jan. 2024.
3. Suong T. Nguyen, Masayasu Taniguchi, S. Kaewma, Megumi Nagahara, Mitsuhiro Takagi and Takeshige Otoi :
Quality and fertilization of frozenthawed porcine spermatozoa separated using migration sedimentation.,
The 50th Conference of the International Embryo Technology Society, Jan. 2024.
4. N. Torigoe, Mutsumi Aihara, Q. Lin, K. Takebayashi, B. Liu, Megumi Nagahara and Takeshige Otoi :
Development of porcine embryos cultured in media irradiated with ultraviolet at 228 and 260 nm wavelength.,
The 50th Conference of the International Embryo Technology Society, Jan. 2024.

Proceeding of Domestic Conference:

1. TORIGOE Nanaka, Lin Qingyi, Liu Bin, Megumi Nagahara and Takeshige Otoi :
細胞保存液を用いたブタ卵母細胞の常温保存後の発育能,
第117回日本繁殖生物学会, Sep. 2024.
2. Megumi Nagahara, Namula Zhao, Lin Qingyi, TAKEBAYASHI Kohki, TORIGOE Nanaka, Liu Bin and Takeshige Otoi :
エルゴチオネイン添加によるブタ卵母細胞の体外成熟能および発育に及ぼす影響,
第117回日本繁殖生物学会, Sep. 2024.
3. Liu Bin, Megumi Nagahara, Namula Zhao, Lin Qingyi, Takebayashi Koki, TORIGOE Nanaka and Takeshige Otoi :
Effect of porcine follicle fluid with the different oxidation stress indices on the meiotic competence and DNA integrity of porcine oocytes,
第117回日本繁殖生物学会, Sep. 2024.
4. Lin Qingyi, Takebayashi Koki, Torigoe Nanaka, Liu Bin, Maki Hirata, Megumi Nagahara and Takeshige Otoi :
Efficient gene editing of pig embryos by combining electroporation and lipofection methods depends on gRNA sequence.,
第117回日本繁殖生物学会, Sep. 2024.
5. Megumi Nagahara, Momo Nakagawa, 吉田 和輝, 森川 繁樹, NAKAYAMA Yuichiroh and Takeshige Otoi :
ウェアラブル血流計を用いた外陰部血流量測定による牛の卵胞個数の予測,
第167回日本獣医学会, Sep. 2024.
6. 佐藤 陽子, Megumi Nagahara, R Ogasawara, Y Obatake, K Kawanishi, H Obatake, K Shibata, A Kinebuchi, Y Higashihara, K Sugaya, R Sambuu, Y Tanighuchi and Takeshige Otoi :
ヤクー牛雑種の雄性不稔に関わる精巣上体細胞サイズの検討,
第46回日本分子生物学会年会, Dec. 2023.
7. TORIGOE Nanaka, Li Qingyi, Liu Bin, Maki Hirata, Megumi Nagahara, Liu Bin and Takeshige Otoi :
Culture method and transfection reagent combinations in genome editing by lipofection in pig zygotes.,
第8回ゲノム編集学会, Jun. 2023.
8. Q Lin, 竹林 滉生, TORIGOE Nanaka, Liu Bin, Maki Hirata, Megumi Nagahara and Takeshige Otoi :
Culture method and transfection reagent combinations in genome editing by lipofection in pig zygotes.,
第8回ゲノム編集学会, Jun. 2023.
9. TORIGOE Nanaka, Li Qingyi, Liu Bin, Maki Hirata, Megumi Nagahara and Takeshige Otoi :
ブタ胚における細胞質内脂肪滴の偏在化がゲノム編集効率に及ぼす影響,
第8回ゲノム編集学会, Jun. 2023.
10. Qingyi Lin, K Takebayashi, N Torigoe, B Liu, Maki Hirata, Megumi Nagahara and Takeshige Otoi :
Culture method and transfection reagent combinations in genome editing by lipofection in pig zygotes.,
第8回ゲノム編集学会, Jun. 2023.