Tokushima University / Educator and Researcher Directory --- Kobayashi, Hotaka

https://sites.google.com/view/h-kobayashi-lab

https://researchmap.jp/hota-koba (researchmap)

https://orcid.org/0000-0002-2363-8838 (ORCID)

○	Life sciences [Neuroscience - general]
○	Life sciences [Molecular biology]
○	Life sciences [Cell biology]

(See https://researchmap.jp/hota-koba (researchmap) for Published Book / Papers / Presentations.)

Book:

1.	泊幸秀 and Hotaka Kobayashi : miRNA経路の全体像, Jul. 2024.

Academic Paper (Judged Full Paper):

1.	Hotaka Kobayashi and Robert H. Singer : A platform for analyzing translational control by RBPs at single-mRNA resolution in cells, Biophysics and Physicobiology, 22, 4, e220025, 2025. (Summary) Translation-the process by which mRNAs are decoded into nascent peptides-is fundamental to life. This process is regulated by various RNA-binding proteins (RBPs), which interact with their target mRNAs. While traditional biochemical approaches have provided valuable insights into translational control, they rely on ensemble measurements of bulk mRNAs in test tubes. Consequently, the behavior of individual mRNAs and their spatiotemporal dynamics in cells during translational control remain largely unexplored. Offering a way to address such limitations, we developed a method for imaging translational control by Argonaute (AGO) proteins, a class of RBPs, at single-mRNA resolution in cells. This method, which employs three-color fluorescence microscopy to detect mRNAs, nascent peptides, and AGO, can also serve as a versatile platform for analyzing translational control by other RBPs. In this protocol, we provide a step-by-step guide for implementing this method to facilitate spatiotemporal studies of translational control at the single-cell and single-mRNA levels. (Link to Publication Site) ● Publication site (DOI): 10.2142/biophysico.bppb-v22.0025 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 41659710 ● Summary page in Scopus @ Elsevier: 2-s2.0-105020961905 (DOI: 10.2142/biophysico.bppb-v22.0025, PubMed: 41659710, Elsevier: Scopus)
2.	元起寧那, 泊幸秀 and Hotaka Kobayashi : Single-molecule Analysis of miRNA Function, Seibutsu Butsuri, 63, 3, 160-162, 2023. (Link to Publication Site) ● Publication site (DOI): 10.2142/biophys.63.160 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390859835690438400 ● Search Scopus @ Elsevier (DOI): 10.2142/biophys.63.160 (DOI: 10.2142/biophys.63.160, CiNii: 1390859835690438400)

Academic Paper (Unrefereed Paper):

1.	Motoki Neina, Tomari Yukihide and Hotaka Kobayashi : Detecting mRNAs and translated peptides with IF-FISH, PSSJ Archives, 17, e114, 2024.

Review, Commentary:

1.	伊藤隼人 and Hotaka Kobayashi : 環状RNAに潜む「逆走」の遺伝暗号, Experimental Medicine, 44, 8, 1226-1227, May 2026.
2.	李沛涵, 泊幸秀 and Hotaka Kobayashi : microRNAによる遺伝子発現抑制と相分離, Journal of Clinical and Experimental Medicine, 292, 4, 287-292, Jan. 2025.
3.	泊幸秀 and Hotaka Kobayashi : microRNAの発見とその歩み, Chemistry, 2024, 12, 32-35, Dec. 2024.

Proceeding of International Conference:

1.	Hotaka Kobayashi : Seeing mRNA vaccines in action with single-molecule imaging, The Third Joint Symposium of AMED SCARDA Japan Initiative for World-leading Vaccine Research and Development Centers, Mar. 2026.
2.	Li Peihan, Motoki Neina, Aoi Satoh, Tomari Yukihide and Hotaka Kobayashi : Single-molecule imaging of poly(A) tail effects on mRNA translation, Asia RNA Club Symposium, Nov. 2025.
3.	Hotaka Kobayashi : Single-molecule imaging of mRNA vaccine action, The Third UTOPIA Symposium, Oct. 2025.
4.	Aoi Satoh and Hotaka Kobayashi : Imaging mRNAs, translation, and RBPs at single-cell and single-molecule resolution, 4D Omics in Development & Disease, Sep. 2025.
5.	Hotaka Kobayashi : Single-molecule imaging of RBP-mediated translational regulation in cells, International Symposium on Cellular Structural Biology, Sep. 2025.
6.	Hotaka Kobayashi : Single-molecule imaging of microRNA function in cells, RNA Tokyo, Jul. 2025.
7.	Hotaka Kobayashi : Imaging expression dynamics of mRNA vaccines, The Second Joint Symposium of AMED SCARDA Japan Initiative for World-leading Vaccine Research and Development Centers, Mar. 2025.
8.	Aoi Satoh and Hotaka Kobayashi : Visualizing translational regulation at single-cell and single-mRNA resolution, The 19th International Symposium of the Institute Network for Biomedical Sciences, Oct. 2024.
9.	Hotaka Kobayashi : Imaging translational regulation at single-cell and single-mRNA resolution, Karolinska Institutet Guest Seminar, Oct. 2024.
10.	Hotaka Kobayashi : Single-molecule imaging of translational regulation in cells, International Symposium on Multifaceted Protein Dynamics, Sep. 2024.
11.	Motoki Neina and Hotaka Kobayashi : Imaging translation at different mRNA ages, International Symposium on Multifaceted Protein Dynamics, Sep. 2024.
12.	Hotaka Kobayashi : Imaging how mRNA vaccines work in cells, The 2nd UTOPIA Symposium, Jun. 2024.

Proceeding of Domestic Conference:

1.	Hotaka Kobayashi : mRNAが蛋白質へと翻訳される様子を細胞内1分子イメージングで見る, JSTさきがけ・ACT-X 合同シンポジウム「若い知性が開拓する新しい生命科学」, Feb. 2026.
2.	Ito Hayato, Morisaki Tatsuya, Hotaka Kobayashi, Stasevich J Timothy and Taguchi Hideki : Visualizing co-translational assembly of translation factor eIF2B subunits in living cells, 2025年東京大学定量生命科学研究所研究交流会, Dec. 2025.
3.	Li Peihan, Motoki Neina, Aoi Satoh, Tomari Yukihide and Hotaka Kobayashi : Single-molecule imaging of poly(A) tail effects on mRNA translation, 2025年東京大学定量生命科学研究所研究交流会, Dec. 2025.
4.	Hotaka Kobayashi : Dissecting microRNA-mediated gene silencing by single-molecule imaging, 第98回日本生化学会大会, Nov. 2025.
5.	Hotaka Kobayashi : mRNA Translation under the Microscope, UTokyo IQB Happy Hour, Sep. 2025.
6.	Hotaka Kobayashi : 細胞内1分子イメージングを用いてmRNAの翻訳制御を見てみると, JST共創の場「国循拠点」第45回若手人材育成セミナー, Aug. 2025.
7.	Hotaka Kobayashi : mRNAワクチンは細胞内でどう働く?を細胞内1分子イメージングで見る, 第22回 UTOPIA Monthly Research Seminar, May 2025.
8.	Hotaka Kobayashi : Single-molecule imaging of translational control in cells, 理化学研究所新領域開拓課題「一分子の科学」若手研究会, May 2025.
9.	Hotaka Kobayashi : 翻訳を見る -細胞内1分子イメージングで捉える，翻訳の時空間情報-, 東京大学大学院新領域創成科学研究科メディカル情報⽣命専攻セミナー, May 2025.
10.	Hotaka Kobayashi : 細胞内1分子イメージングを武器にmicroRNAの謎に迫る, 2024年度徳島大学先端酵素学研究所シンポジウム, Aug. 2024.
11.	Hotaka Kobayashi : When and where do microRNAs work in cells?, 第76回日本細胞生物学会大会, Jul. 2024.
12.	元起寧那, 泊幸秀 and Hotaka Kobayashi : Imaging translation at different mRNA ages, 2023年東京大学定量生命科学研究所研究交流会, Dec. 2023.
13.	Hotaka Kobayashi : ストレス応答を支えるmicroRNA-その背後にある不思議な現象を複合的なオミクス解析から紐解く-, 第17回日本臨床ストレス応答学会大会, Nov. 2023.
14.	Hotaka Kobayashi : ncRNAが機能する様子をこの目で見てみたい, 第63回生物物理若手の会夏の学校, Sep. 2023.
15.	Motoki Neina, Tomari Yukihide and Hotaka Kobayashi : Imaging translation at different mRNA ages, 第24回日本RNA学会年会, Jul. 2023.
16.	Hotaka Kobayashi : In-cell single-molecule imaging of protein synthesis and its regulation, 第23回日本蛋白質科学会年会, Jul. 2023.

細胞伸長部位を「反応場」とするアクチン局所翻訳の実証と開拓 (Project/Area Number: 25K24896 )
翻訳と反応場から考える蛋白質の新しい社会像の領域推進 (Project/Area Number: 25K24895 )
microRNAの時空間動態を細胞内1分子イメージングで解き明かす (Project/Area Number: 25K02208 )
細胞伸長部位を「反応場」とするアクチン局所翻訳の実証と開拓 (Project/Area Number: 25H01439 )
翻訳と反応場から考える蛋白質の新しい社会像の領域推進 (Project/Area Number: 25H01438 )
高時空間解像度イメージングという視点から翻訳の典型的・非典型的な制御機構に迫る (Project/Area Number: 23H04251 )
Dissecting the mechanism for autophagic degradation of empty Argonaute (Project/Area Number: 17H06616 )
Search by Researcher Number (80802975)

Associate Professor : Kobayashi, Hotaka

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Book:

Academic Paper (Judged Full Paper):

Academic Paper (Unrefereed Paper):

Review, Commentary:

Proceeding of International Conference:

Proceeding of Domestic Conference:

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)