【Introduction】The survival rate in patients with HIV infection and acquired immunodeficiency syndrome (AIDS) has been improved dramatically due to the advances in anti-HIV drug therapy, while aging-associated complications become a critical issue. The incidence of sudden occurrence of AIDS without prior detection of HIV infection, so called ``Ikinari AIDS'', still remains high. 【Objective】We retrospectively analyzed the incidence and clinical characteristics of HIV/AIDS patients in both Tokushima University Hospital and Tokushima Prefectural Central Hospital. 【Results】Eighty four patients (74 males and 10 females) with a median age of 39 years old (range 16 - 85) were enrolled. Thirty-four patients (40.5%) were diagnosed with ``Ikinari AIDS'' from 2001 to 2020. All 4 patients were diagnosed with ``Ikinari AIDS'' after 2020. AIDS-defining illnesses were diagnosed as follows ; pneumocystis pneumonia in 21 cases, CMV infection in 8 cases and candidiasis in 6 cases. All patients over 60 years old were suffered from AIDS. Other complications included syphilis in 17 cases, hepatitis B infection in 12 and herpes zoster in 7. 【Discussion/Conclusion】In Tokushima, the incidence rate of ``Ikinari AIDS'' appeared to be higher than that of national average. COVID - 19 pandemic hampered the public health care services of awareness-raising activity for HIV infection and telephone consultations about HIV, which may become more lease asymptomatic HIV patients without diagnosis. For early diagnosis of HIV/AIDS, it is becoming more important to share information to make early screening of HIV infection among medical staffs, such as medical doctors, dentists, nurses, pharmacists and MSWs.
【Introduction】Congenital hemophilia is a category of hemorrhagic disease caused by a genetic defect in the production of coagulation factors. It is treated by administering regular coagulation factor injections on an ongoing basis. Hemophilia is a hereditary illness, often causing social and psychological problems as a result of the disease. To analyze the objective effects of hemophilia, we conducted a retrospective analysis in Tokushima University Hospital. 【Result】All 23 cases were men between the ages of20and72. Hemophilia A was present in17cases, and hemophilia B was present in six. Nineteen out of 23 cases were severe, and the others were intermediate. Medical assessments were conducted at pediatrics in seven cases and hematology in 16 cases. Adoption of the self-injection technique was not realized in five cases. Seventeen cases were complicated by hemophilic arthropathy, seven with human immunodeficiency virus(HIV), and 12 with hepatitis C virus. Eight participants were unemployed, and17were unmarried. 【Discussion】 Many adult hemophilia patients still visit pediatrics in our hospital. Hemophilia in the period of growth between adolescence and young adulthood is often accompanied by life-altering events such as entering higher education, marriage, and work experience. Therefore, collaboration among professionals of multiple occupations, such as doctors, nurses, pharmacists, medical social workers, and clinical psychologists, is essential. Furthermore, there are many cases of HIV and hepatitis C virus infections complicating hemophilia study due to the stigma surrounding HIV-tainted blood. 【Conclusion】It is imperative that we establish a long-term, sustainable, and multi-disciplinary transitional care and medical support system for patients and their families.
Toshiyuki Suge, Ishikawa Kunio and Takashi Matsuo : Changes in the crystallinity of hydroxyapatite powder and structure of enamel treated with several concentrations of ammonium hexafluorosilicate, American Journal of Dentistry, Vol.25, No.5, 299-302, 2012.
(Summary)
To evaluate the effects of changing concentrations of ammonium hexafluorosilicate [SiF: (NH4)2SiF6] solution on the crystallinity of hydroxyapatite powder (HAP) and structure of human enamel in order to overcome the tooth discoloration caused by diamine silver fluoride [AgF: (NH3)2AgF] application. HAP was treated with several concentrations of SiF solution (from 10 to 19,400 ppm) for 5 minutes. The crystallinity of the HAP before and after SiF treatment was then measured by powder X-ray diffraction (XRD) analysis. The angular width (beta) of the 002 diffraction peak was measured at 1/2 the height of the maximum intensity. Also, enamel specimens were prepared from a human extracted tooth. Several concentrations of SiF solution were applied to polished or phosphoric acid etched enamel specimens. The enamel surface was then observed using scanning electron microscopy (SEM). XRD peaks became sharper after SiF treatment indicating that the crystallinity of apatite powder was increased. The 1/beta value was increased from 2.8 +/- 0.1 to 4.3 +/- 0.1 after treatment with 1,000 ppm SiF solution. The amount of CaF2 formed in HAP was gradually increased with increasing concentrations of SiF solution. TheXRD pattern was consistent with CaF2 in case of over 9,000 ppm SiF solution. SEM photographs demonstrated that exposed enamel rods with acid etching were filled with CaF2-like precipitate after SiF treatment regardless of the concentration of SiF solution.
Shibata Shingo, Toshiyuki Suge, Tomoko Kimura, Ishikawa Kunio and Takashi Matsuo : Antibacterial activity of ammonium hexafluorosilicate solution with antimicrobial agents for the prevention of dentin caries, American Journal of Dentistry, Vol.25, No.1, 31-34, 2012.
(Summary)
This study evaluated the antibacterial activity of the SiF solution with the addition of antibacterial agents on a Streptococcus mutans biofilm. Various antibacterial SiF solutions were prepared by adding chlorhexidine, cetylpyridinium chloride, isopropyl methylphenol, or epigallocatechin gallate. Hydroxyapatite pellets treated with several SiF solutions were immersed in BHI inoculated with S. mutans standardized suspension. The number of S. mutans cells adhered to each pellet was evaluated. SiF with the addition of CPC was the most effective for reducing the adherence of bacteria and inhibiting the formation ofbiofilm, showing the same level as AgF, In contrast, the addition of other antibacterial agents to SiF reduced the original antibacterial activity of SiF solution.
Shinya Horiuchi, Shingo Kuroda, Masahiro Hiasa, Toshiyuki Suge, Seitaro Saku, Kenichi Hamada, Takashi Matsuo, Kenzo Asaoka and Eiji Tanaka : Reinforcement of bond strength of self-etching orthodontic adhesive., The Angle Orthodontist, Vol.82, No.1, 30-35, 2011.
(Summary)
Abstract Objective: To determine the reinforcement of bond strength of a self-etching system by applying a pretreatment agent. Materials and Methods: Sixty extracted human premolars were used in this study. The enamel surfaces were treated with four pretreatment agents?phosphoric acid, polyacrylic acid, citric acid, and ammonium hexafluorosilicate (SiF)?and were examined under a scanning electron microscope. Afterward, orthodontic brackets were bonded with a self-etching adhesive system (n ?=? 10 for each agent), and shear bond strength was measured through a debonding process. The adhesive remnant index (ARI) was also assessed. Results: Enamel surfaces treated with polyacrylic acid seemed almost the same as intact enamel. Treatment with SiF induced slight shallow depressions compared with the intact enamel. On the other hand, enamel surfaces treated with citric acid and phosphoric acid showed severe etching patterns. All pretreatments increased the bond strength, but SiF-treated specimens revealed the greatest strength (12.201 ? 1.048?MPa), followed by polyacrylic acid (12.030 ? 2.103?MPa). The control group with no pretreatment showed the least strength (9.078 ? 1.678?MPa). All pretreatments increased ARI score compared with the control group. Conclusions: Surface conditioning before bracket adhesion could reinforce the bond strength of the self-etching adhesive system, resulting in a more reliable bonding system.
Toshiyuki Suge, Akiko Kawasaki, Ishikawa Kunio, Takashi Matsuo and Ebisu Shigeyuki : Effects of ammonium hexafluorosilicate concentration on dentin tubule occlusion and composition of the precipitate, Dental Materials, Vol.26, No.1, 29-34, 2010.
(Summary)
Ammonium hexafluorosilicate [SiF: (NH(4))(2)SiF(6)] was prepared in order to overcome the tooth discoloration caused by diamine silver fluoride [AgF: (NH(3))(2)AgF] application. We employed a single concentration of SiF solution in our previous study; therefore, it is still unclear how the concentration of SiF solution affects the occlusion of dentin tubules and composition of the precipitate. The aim of this study was to evaluate the effects of changing the concentration of SiF on its clinical use as a dentin hypersensitivity treatment. To simulate dentin tubules subject to dentin hypersensitivity, dentin disks were treated with EDTA for 2 min. Then, the disks were treated with several concentrations of SiF solution (from 100 to 19,400 ppm) for 3 min. The occlusion of dentin tubules was evaluated using scanning electron microscopy (SEM), and the composition of the precipitate formed in the tubules after SiF treatment was assessed using energy dispersive X-ray analysis (EDXA). SEM photographs demonstrated that dentin tubules after treatment with SiF were occluded homogeneously and fully regardless of the concentration of SiF solution. The Ca/P molar ratio of the precipitate formed in dentin tubules after SiF treatment was increased with the concentration of SiF solution. It was concluded that the capacity to occlude dentin tubules was the same regardless of the concentration of SiF solution. However, the composition of the precipitate formed in the tubules was dependent on the concentration of SiF solution.
Toshiyuki Suge, 石川 邦夫, Takashi Matsuo and 恵比須 繁之 : フッ化ジアミンシリケートの象牙質知覚過敏症治療剤への応用―抜去歯を用いた象牙細管封鎖能の検討―, The Japanese Journal of Conservative Dentistry, Vol.50, No.3, 313-320, 2007.
(Summary)
Diamine silver fluoride (Saforide^[ !R]) is used clinically to prevent the progress of dental caries and desensitize hypersensitive teeth. Unfortunately, diamine silver fluoride stains teeth black due to silver precipitation; therefore, it is mainly applied to deciduous teeth. To overcome this drawback, ammonium hexafluorosilicate(SiF:(NH_4)_2SiF_6)was prepared. Silica was employed instead of silver because it does not change the color of teeth, and is well known to induce apatite formation from simulated body fluids. The purpose of this study was to evaluate the occluding ability and duration of dentin tubule occlusion after SiF treatment in a simulated oral environment. The occluding ability of SiF was evaluated using human dentin disks. The surface of dentin disks was observed with a scanning electron microscope (SEM)immediately after SiF treatment and 7 days after immersion in synthetic saliva. SEM observation showed that open dentin tubules were occluded completely with silica-calcium phosphate precipitate immediately after SiF treatment. In addition, newly formed crystals covered the dentin surface 7 days after immersion in synthetic saliva. EDXA analysis showed that the precipitate formed in the dentin tubules after SiF treatment contains silica, calcium and phosphorous indicating silica-calcium phosphate complex. The Ca/P molar ratio of the precipitate formed with SiF treatment was 2.02, and the value gradually decreased after immersion in synthetic saliva. In contrast, the Ca/P molar ratio of newly formed crystals at the dentin surface showed a relatively constant value(Ca/P=1.16-1.28)throughout the experimental period. It seems that calcium-deficient apatite may be precipitated at the dentin surface due to the Ca/P ratio being significantly lower than that of intertubular dentin. SiF treatment induced calcium phosphate precipitation from synthetic saliva; therefore, it has a continuous effect on dentin tubule occlusion under a simulated oral environment.
Diamine silver fluoride [AgF: (NH(3))(2)AgF] has been used clinically in Japan, as it reduces dental caries and dentin hypersensitivity. However, AgF stains the teeth black due to silver precipitation. To overcome this drawback, the authors prepared ammonium hexafluorosilicate [SiF: (NH(4))(2)SiF(6)], which does not stain the teeth, and SiF occluded open dentin tubules completely with silica-calcium phosphate precipitate. The aim of this study was to evaluate the duration of dentin tubule occlusion after SiF treatment in a simulated oral environment. To simulate dentin tubules subject to dentin hypersensitivity, dentin disks were treated with EDTA for 2 min. The disks were treated with 0.476 mol/L SiF for 3 min, and then the disks were immersed in synthetic saliva, which was regularly replenished to maintain its ionic concentration, for up to 7 days. The occluding ability of the dentin tubules was evaluated using scanning electron microscopy (SEM), and the hydraulic conductance was measured following Pashley's method at regular intervals. SEM photographs demonstrated that dentin tubules were occluded homogeneously and completely with the precipitate at 7 days after treatment with SiF. In addition, newly formed calcium phosphate precipitate was present at the dentin surface. The dentin permeability showed a consistently low value throughout the experimental period. The values immediately after SiF treatment and 7 days after immersion were 11.9+/-3.7% and 7.9+/-2.9%, respectively. Ammonium hexafluorosilicate is useful for the treatment of dentin hypersensitivity, since ammonium hexafluorosilicate induced calcium phosphate precipitation from the saliva; therefore, it has a continuous effect on dentin tubules occlusion under a simulated oral environment.
Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and Shigeyuki Ebisu : Effect of ammonium hexafluorosilicate on dentin tubule occlusion for the treatment of dentin hypersensitivity, American Journal of Dentistry, Vol.19, No.4, 248-252, 2006.
(Summary)
To evaluate the occluding ability of ammonium hexafluorosilicate (SiF). Dentin disks prepared from human extracted teeth were grouped as follows to prepare different situations of dentin hypersensitivity: (1) those sonicated for 20 minutes; (2) those treated with 0.5 mol/L EDTA for 2 minutes; (3) those treated with 6% citric acid for 2 minutes; (4) those treated with 50% citric acid for 2 minutes. Then, SiF or diamine silver fluoride (AgF) was applied to the dentin disks and the dentin tubule occlusion was observed with scanning electron microscopy (SEM). The percent of open tubules before and after SiF or AgF treatment were measured by NIH image using SEM photographs. Also, the dentin permeability was measured. SEM micrographs demonstrated that the dentin tubules were completely occluded by the precipitate after SiF treatment. Also, the dentin permeability was reduced to 10.3%. In contrast, most of the dentin tubules remained open after AgF treatment. EDXA analysis showed that the precipitate in the dentin tubules that forms after SiF treatment contains Si, Ca and P, indicating a silica-calcium phosphate complex.
Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and Shigeyuki Ebisu : Effects of plaque control on the patency of dentinal tubules-an in vivo study in beagle dogs, Journal of Periodontology, Vol.77, No.3, 454-459, 2006.
(Summary)
The aim of this in vivo study was to evaluate the effects of plaque control on the patency of dentinal tubules using vital teeth of beagle dogs. Class V cavities were prepared on the cervical areas of the mandibular and maxillary molars in each dog with a diamond point. To simulate the state of dentinal hypersensitivity, the teeth were etched with 50% citric acid for 2 minutes to obtain patent dentinal tubules. Plaque control was achieved by brushing the left-side teeth every day, whereas no plaque control was performed for the right-side teeth. A dentin biopsy was performed after 1, 2, and 3 weeks using the cylindrical diamond point to obtain dentin specimens. In the plaque control group, some of the dentinal tubules were occluded with precipitate (Ca/P=1.49), and the diameter of the dentinal tubules decreased from 2.42+/-0.33 microm (mean+/-SD) to 1.11+/-0.51 microm after 7 days, although most of the dentinal tubules remained open. In contrast, no precipitate was observed in the dentinal tubules of the non-plaque control group. Also, the diameter of the dentinal tubules increased from 2.42+/-0.33 to 2.9+/-0.49 microm, due to the demineralization of the peritubular and intertubular dentin. Plaque control plays a key role in reducing the patency of dentinal tubules and, therefore, might promote the natural repair of dentinal hypersensitivity.
Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and SHigeyuki Ebisu : Comparison of the occluding ability of dentinal tubules with different morphology between calcium phosphate precipitation method and potassium oxalate treatment, Dental Materials Journal, Vol.24, No.4, 522-529, 2005.
(Summary)
The aim of this study was to evaluate the occluding ability of calcium phosphate precipitation (CPP) method and potassium oxalate treatment when each method was applied to dentin disks with different surface morphology. Occluding ability was observed by scanning electron microscopy. Irrespective of the diameter of the dentinal tubules, the CPP method showed a consistent occluding ability for dentinal tubules at the dentin surface, and that the depths of the precipitate formed in the dentinal tubules by CPP method were not significantly different. In contrast, the occluding ability of potassium oxalate treatment was reduced with increasing diameter of the dentinal tubules. However, the reduction of the occluding ability of potassium oxalate treatment was more markedly affected by the demineralization of dentin surface. Since the CPP method showed a consistent occluding ability irrespective of the diameter of the dentinal tubules, it is suggested that the CPP method would be a useful means for treating dentin hypersensitivity.
Akiko Kawasaki, Toshiyuki Suge, Kunio Ishikawa, Kazumi Ozaki, Takashi Matsuo and Shigeyuki Ebisu : Ammonium hexafluorosilicate increase acid resistance of bovine enamel and dentine, Journal of Materials Science. Materials in Medicine, Vol.16, No.5, 461-466, 2005.
(Summary)
Although diamine silver fluoride (AgF: (NH3)2AgF) stains teeth black, it is known as a very effective agent to prevent the dental caries progress. In order to find another fluoride that has a similar anticariogenic effect without changing tooth color, we prepared ammonium hexafluorosilicate (SiF: (NH4)2SiF6), in which the silver of AgF is replaced with silicon. In this study, the anticariogenic effect of SiF was evaluated using bovine teeth. Fluoride solutions, SiF, AgF, acidulated phosphate fluoride (APF), and sodium fluoride (NaF), were applied to bovine enamel and dentine blocks, and the depth of demineralization was measured after exposure to a demineralizing solution for 24 h. Also, fluoride was applied to a simulated dentine caries specimen to evaluate the caries progress-preventing ability. For the dentine specimens, mineral loss (Delta Z) was also measured with microradiography. We found that SiF treated enamel showed better acid resistance than specimens treated with NaF or APF. AgF treated enamel also showed similar acid resistance, but was stained black. SiF and AgF treated caries-affected dentine showed reduced demineralization when exposed to a demineralization solution for 24 h. Mineral loss (Delta Z) was reduced to 85% and 75%, respectively. Although the acid resistance of the SiF treated teeth was inferior to that of the AgF treated teeth, we consider that SiF has good potential as anticariogenic agent, since it increased acid resistance without changing tooth color.
Toshiyuki Suge, Ishikawa Kunio, Akiko Kawasaki, Takashi Matsuo and EBISU Shigeyuki : Effects of pre- or post- application with calcium chloride on occluding ability of potassium oxalate for the treatment of dentin hypersensitivity, American Journal of Dentistry, Vol.18, No.2, 121-125, 2005.
(Summary)
To evaluate whether calcium ion supply using 1-6 mol/L CaCl2 solution could enhance the occluding ability of dentin tubules with 30% potassium oxalate treatment. Calcium chloride solution (1-6 mol/L) was applied to dentin disks before or after application of 30% potassium oxalate solution. Occluding ability after potassium oxalate treatment was evaluated with scanning electron microscopic (SEM) observation and measurement of dentin permeability. In addition, the composition of the precipitate formed when mixing potassium oxalate and calcium chloride was analyzed with a powder x-ray diffractometer (XRD). SEM observations revealed that the dentin tubules were occluded homogeneously and completely with the precipitate when calcium chloride solution was applied before or after potassium oxalate treatment. However, the depth of the precipitate in dentin tubules from the dentin surface became shallower when pre-treated with calcium chloride before potassium oxalate. Although dentin permeability was greatly reduced in both groups, no significant difference could be observed between samples with and without calcium chloride application.
Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Kazuomi SUZUKI, Takashi Matsuo, Yuichiro NOIRI, Satoshi IMAZATO and Shigeyuki EBISU : Calcium phosphate precipitation method for the treatment of dentin hypersensitivity, American Journal of Dentistry, Vol.15, No.4, 220-226, 2002.
(Summary)
To evaluate the feasibility of the calcium phosphate precipitation (CPP) method as a treatment for dentin hypersensitivity using vital teeth of beagle dogs. Also, gingival tissue response to two types of CPP methods was examined histologically. Dentin tubules of the dogs' vital teeth were exposed by shallow cavity preparation followed by etching with 50% citric acid to simulate the condition of hypersensitive dentin. After CPP treatment was applied to the vital tooth, the dentin surface and longitudinal sections were observed by SEM to evaluate the occluding ability of the CPP method. The precipitate was also analyzed by energy-dispersive X-ray microanalysis to obtain compositional information. The gingival tissue before and after CPP method was examined histologically with light microscopy. Dentin tubules were occluded homogeneously and completely with an apatitic mineral after application of the CPP treatment in vital teeth. However, the depth of the precipitate in dentin tubules from the dentin surface was approximately half that seen in extracted teeth. No histological change was observed in gingival tissues when NaHCO3 was used as a post-treatment solution of the CPP method, whereas another CPP method using NaOH solution resulted in atrophy and degeneration of the epithelium of gingival tissue.
Akiko Kawasaki, Kunio ISHIKAWA, Toshiyuki Suge, Hirotoshi SHIMIZU, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU : Effects of plaque control on the patency and occlusion of dentine tubules in situ., Journal of Oral Rehabilitation, Vol.28, No.5, 439-449, 2001.
(Summary)
To elucidate the nature of dentine hypersensitivity, the effects of plaque control on the patency and occlusion of dentinal tubules were investigated systematically in situ using human dentine slabs embedded in partial dentures. The dentine slabs were divided into three groups. In group I, the dentine slabs were kept in an oral cavity without plaque control. In group II, plaque was removed mechanically by brushing. Plaque control was carried out chemically using chlorhexidine in group III. After being kept in the oral cavities for 1, 2 and 3 weeks, the slabs were removed from the partial denture, followed by SEM observation to determine the morphological changes of the dentinal tubules. When no attempt was made to remove plaque, the diameter of tubule orifices increased to 390% of the original values within 3 weeks. In contrast, dentinal tubules were found to be occluded, i.e. the tubule orifices became <20% of the original value within 1 week when plaque control efforts were made, using either method of plaque control. We conclude that plaque control plays one of the key roles in the patency versus occlusion of dentinal tubules, and thus in the aetiology and natural reparative process of dentine hypersensitivity.
Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU : Evaluation of post-treatment solutions for clinical use with the calsium phosphate precipitation method, Journal of Dentistry, Vol.27, No.7, 487-496, 1999.
(Summary)
We have proposed a calcium phosphate precipitation (CPP) method that occludes dentine tubules with apatitic minerals for the treatment of dentine hypersensitivity. The current CPP method uses 1 mol/l NaOH as the post-treatment solution. However, its high pH is not desirable for clinical use. The aim of this study was to evaluate several solutions, especially buffer solutions, as post-treatment solutions for the CPP method. The CPP solution was mixed with several post-treatment solutions at various mixing ratios in test tubes. The precipitates were collected, and freeze-dried in a vacuum. Their weights were measured to evaluate the feasibility of buffer solution for the post-treatment solutions. Among the solutions, we selected one buffer solution and have done further evaluation using human dentine disks. The degree of occlusion of dentine tubules was evaluated by scanning electron microscopic observation and measurement of dentine permeability. Also, the composition of the precipitate was analyzed using energy-dispersive X-ray microanalysis and powder X-ray diffraction. Among the solutions, 1 mol/l NaHCO3 gave sufficient amounts of precipitate at a relatively mild pH, and thus was considered to be a good candidate for a post-treatment solution. Scanning electron microscopic observation showed that dentine tubules were occluded with the precipitate to a depth of approximately 10 microns from the surface when the CPP treatment was done twice, and dentine permeability was reduced to 98.8%. The precipitate was dicalcium phosphate dihydrate (DCPD; CaHPO4-2H2O) when the post-treatment solution was free of NaF. In contrast, the precipitate was an apatitic mineral with CaF2 as a by-product when the post-treatment solution contained NaF. We concluded that 1 mol/l NaHCO3 containing 0.3 mol/l NaF would be suitable as the post-treatment solution for the CPP method as it has a relatively mild pH and occludes dentinal tubules well.
Masahiro YOSHIYAMA, Toshiyuki Suge, Akiko Kawasaki and Shigeyuki EBISU : Morphological characterization of tube-like structures in hypersensitive human radicular dentine, Journal of Dentistry, Vol.24, No.1-2, 57-63, 1996.
(Summary)
The objective of this work was to determine if there were differences in the distribution of tube-like structures within the tubules of biopsies from hypersensitive versus non-sensitive human cervical radicular dentine. Ten pairs of dentine were made from the exposed sensitive and non-sensitive radicular dentine of nine adult patients complaining of dentine sensitivity. The biopsies were made with a small hollow diamond bur. The specimens were submitted to HCl-collagenase treatment to partially digest the surface to permit visualization of subsurface structures. Half of the specimens were embedded in resin to facilitate longitudinal sectioning, and the other half were processed with embedding for SEM and EDS observation. Biopsies from hypersensitive regions exhibited hollow, tube-like structures within the lumina of dentinal tubules in 75.8 +/- 9.4%) ( mean +/- S.D.) of the tubules. In biopsies of non-sensitive areas of the same teeth, these tube-like structures were only seen in 20.4 +/- 8.5% of the tubules. In specimens that were embedded prior to treatment with HCl-collagenase, resin tags penetrated many of the tubules from hypersensitive dentine but few of the tubules from non-sensitive dentine. It is speculated that tube-like structures found in hypersensitive dentine play a role in maintaining the patency of the tubules which may prevent physicochemical processes from spontaneously occluding the tubules, thereby maintaining dentine sensitivity.
Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Effects of hexafluorosilicate on the precipitate composition and dentine tubule occlusion by calcium phosphate, Journal of Dentistry, Vol.24, No.6, 429-434, 1996.
(Summary)
To improve the calcium phosphate precipitation (CPP) method for the occlusion of dentine tubules with calcium phosphate, the addition of calcium hexafluorosilicate (CaSiF6) to CPP solution was evaluated in vitro with respect to its occluding capacity and the composition of the precipitate. The occlusion of dentine tubules was evaluated by SEM observations and by measurements of dentine permeability. The composition of the precipitate was determined by measuring the calcium to phosphorus (Ca/P) ratio of the precipitate in dentine tubules by energy dispersive X-ray spectroscopy (EDS). The addition of CaSiF6 to the CPP solution resulted in an increase of the Ca/P ratio in the precipitate not only on the dentine surface but also inside the dentine tubules; indicating that the precipitate became more apatitic in nature. The addition of CaSiF6 had no effect on occluding capacity in terms of measurements of dentine permeability or SEM observations. Dentine permeability decreased to approximately 4% of pretreatment values and the dentine tubules were occluded for approximately 10-15 microns from the dentine surface. It is concluded that the addition of CaSiF6 to CPP solution was desirable, since it provided a more apatitic precipitate in the dentine tubules, not only on the dentine surface but also inside the tubules, and there were no drawbacks with respect to its occluding capacity.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Effects of fluoride on the calcium phosphate pricipitation method for dentinal tubules occlusion, Journal of Dental Research, Vol.74, No.4, 1079-1085, 1995.
20.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Duration of dentinal tubule occlusion formed by calcium phosphate precipitation method: in vitro evaluation using synthetic saliva, Journal of Dental Research, Vol.74, No.10, 1079-1714, 1995.
21.
Kunio Ishikawa, Toshiyuki Suge, Masahiro Yshiyama, Akiko Kawasaki, Kenzo Asaoka and Shigeyuki Ebisu : Occlusion of dentinal tubules with calcium phosphate using acidic calcium phosphate solution followed by neutralization., Journal of Dental Research, Vol.73, No.6, 1197-1204, 1994.
22.
Masahiro YOSHIYAMA, Toshiyuki Suge, Akiko Kawasaki and Shigeyuki EBISU : Microstructural changes of cervical abrasive dentin by application of primers, The Japanese Journal of Conservative Dentistry, Vol.37, No.2, 404-410, 1994.
23.
Masahiro YOSHIYAMA, Toshiyuki Suge, Akiko Kawasaki, Reiko OGIMOTO and Shigeyuki EBISU : Mechanisums of dentin hypersensitivity, --- ultrastructural characterization of both success and failure cases after topical aplication of aresin liner ---, The Japanese Journal of Conservative Dentistry, Vol.36, No.1, 200-205, 1993.
Academic Paper (Unrefereed Paper):
1.
Toshiyuki Suge : Etiology and treatment method for dentin hypersensitivity, Shikoku Dental Research, Vol.26, No.2, 55-60, 2014.
(Tokushima University Institutional Repository: 109845)
2.
Toshiyuki Suge and Takashi Matsuo : Effects of ammonium hexafluorosilicate concentration on crystallinity of hydroxyapatite powder and enamel, Key Engineering Materials, Vol.529-530, 526-530, 2013.
Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and Shigeyuki Ebisu : A new treatment method for dentin hypersensitivity: application of ammonium hexafluorosilicate solution, Dental tribune, Asia Pacific edition, Vol.4, No.12, 10-12, 2006.
4.
Masahiro YOSHIYAMA, Toshiyuki Suge, Akiko Kawasaki and Shigeyuki EBISU : Process-like structures in the tubules of hypersensitive human dentine., Archives of Oral Biology, Vol.39, No.Suppl, 153, 1994.
Review, Commentary:
1.
Daisuke Takegawa, Kazuhide Yonekura, Hitomi Kuramoto, Yumika Ida, Yuki Hosokawa, Ikuko Hosokawa, Yoshitaka Hosokawa, Toshiyuki Suge, Tadashi Nakanishi and Keiichi Hosaka : Clinical Key Points of Class II Direct Composite Restoration, Journal of Oral Health and Biosciences, Vol.36, No.1, 8-12, Sep. 2023.
Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA and Takashi Matsuo : Effect of ammonium hexafluorosilicate on acid resistance of enamel and dentin, Sydney, May 2004.
2.
Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa and Takashi Matsuo : Occlusion of dentinal tubules with ammonium hexafluorosilicate for treatment of dentin hypersensitivity, 7th World Biomaterials Congress, Sydney, May 2004.
3.
Akiko Kawasaki, Toshiyuki Suge, Kunio Ishikawa, Kazumi Ozaki, Takashi Matsuo and Shigeyuki Ebisu : Ammonium hexafluorosilicate increase acid resistance of bovine enamel and dentin, Journal of Dental Research, Vol.82, No.Special Issue B, B-71, Goteborg, Jun. 2003.
4.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Takashi Matsuo and Shigeyuki Ebisu : Duraiton of ammonium hexafluorosilicate for dentinal tubules occlusion, Journal of Dental Research, Vol.82, No.Special Issue B, B-47, Goteborg, Jun. 2003.
5.
Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA, Kazumi Ozaki and Takashi Matsuo : Ammonium hexafluorosilicate increased acid resistance of bovine enamel and dentin, Transactions of the fourth international congress on dental materials, Vol.16, 195, Honolulu, Jan. 2002.
6.
Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Takashi Matsuo and Shigeyuki EBISU : Effects of ammonium hexafluorosilicate on dental tubules' occlusion, Transactions of the fourth international congress on dental materials, Vol.16, 253, Honolulu, Jan. 2002.
7.
Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU : Effects of ammonium hexafluorosilicate on occluion of dentinal tubules, Journal of Dental Research, Vol.80, No.Special Issue, 750, Makuhari, Jun. 2001.
Akiko Kawasaki, ISHIKAWA Kunio, Toshiyuki Suge, SUZUKI Kazuomi, Takashi Matsuo and EBISU Shigeyuki : In vivo evaluation of plaque control on dentin hypersensitivity, Journal of Dental Research, Vol.77, No.Special Issue, 571, Nice, Jun. 1998.
10.
Toshiyuki Suge, ISHIKAWA Kunio, Akiko Kawasaki, SUZUKI Kazuomi, Takashi Matsuo and EBISU Shigeyuki : Comparison of dentinal tubules occlusion-CPP, Resin and Potassium Oxalate, Journal of Dental Research, Vol.77, No.Special Issue, 651, Nice, Jun. 1998.
11.
Shigeyuki Ebisu, T Yasunaga, Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Kenzo Asaoka and Takashi Matsuo : Acid resistance of bovine enamel after calcium phosphate precipitation method using dogs, Journal of Dental Research, Vol.76, No.Special Issue, 180, Orlando, Mar. 1997.
12.
Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu : Effects of plaque control on the occlusion of dentin tubules, Journal of Dental Research, Vol.76, No.Special Issue, 180, Orlando, Mar. 1997.
13.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu : Acid resistance of bovine enamel after calcium phosphate precipitation method, Journal of Dental Research, Vol.76, No.Special Issue, 97, Orlando, Mar. 1997.
14.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Calcium phosphate precipitation method using buffer for post-treatment solution, Journal of Dental Research, Vol.75, No.Special Issue, 279, San Francisco, Mar. 1996.
15.
Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Effects of calcium phosophate precipitation method on crystalinity of apatite, Journal of Dental Research, Vol.75, No.Special Issue, 279, San Francisco, Mar. 1996.
16.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Duration of calcium phosphate precipitation method for dentinal tubules occlusion, Journal of Dental Research, Vol.74, No.Special Issue, 423, Singapore, Jun. 1995.
17.
Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Masahiro Yoshiyama, Kenzo Asaoka and Shigeyuki Ebisu : Effecs of fluoride on CPP method for dentin hypersensitivity treatment, Journal of Dental Research, Vol.74, No.Special Issue, 423, Singapore, Jun. 1995.
Proceeding of Domestic Conference:
1.
Toshiyuki Suge, Naoto Noguchi, Takashi Matsuo, Katsuhiko Yoshimoto and Tetsuo Ichikawa : 日本大学松戸歯学部臨床実習視察報告, 第42回四国歯学会例会, Mar. 2013.
Toshiyuki Suge, Akiko Kawasaki, Takashi Matsuo, Kunio ISHIKAWA and Shigeyuki EBISU : Duration of ammonium hexafluorosilicate for dentinal tubules occlusion, The Japanese Journal of Conservative Dentistry, Vol.47, 100, Nov. 2004.
4.
Toshiyuki Suge, Akiko Kawasaki, Kazumi Ozaki, Takashi Matsuo, 石川 邦夫 and 恵比須 繁之 : Effects of ammonium hexafluorosilicate on acid resistance of bovine teeth, 第118回 日本歯科保存学会春季学会, Jun. 2003.
Toshiyuki Suge, Akiko Kawasaki, Takashi Matsuo, 石川 邦夫, 鈴木 一臣 and 恵比須 繁之 : Effects of ammonium hexafluorosilicate on occlusion of dentinal tubules for the treatment of dentin hypersensitivity, The Japanese Journal of Conservative Dentistry, Vol.44, 114, Jun. 2001.
7.
Toshiyuki Suge, Akiko Kawasaki, Takashi Matsuo, 石川 邦夫, 鈴木 一臣 and 恵比須 繁之 : Effects of calcium chloride on potassium oxalate treatment for dentin hypersensitivity, The Japanese Journal of Conservative Dentistry, Vol.43, 102, Apr. 2000.
8.
Keiko Fujinaka, 吉田 佳子, 松岡 希実, Akiko Kawasaki, Toshiyuki Suge and Takashi Matsuo : Localization and identification of bacteria in the apices of roots with refractory apical periodontitis, The Japanese Journal of Conservative Dentistry, Vol.42, 57, Nov. 1999.
松岡 希実, 吉田 佳子, Akiko Kawasaki, Toshiyuki Suge, 野杁 由一郎 and Takashi Matsuo : An immunolhistological study on the localization of selected bacteria in the apexies of infected root canal systems, The Japanese Journal of Conservative Dentistry, Vol.41, 71, Jun. 1998.
11.
Akiko Kawasaki, Toshiyuki Suge, Takashi Matsuo, 石川 邦夫 and 恵比須 繁之 : Effects of Calcium Phosphate Precipitation Method on the Crystallinity and Acid Resistance of Enamel, The Japanese Journal of Conservative Dentistry, Vol.39, 118, Jun. 1997.
12.
Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu : Effect of apatite forming method on the crystallinity of hydroxyapatite powder, Transaction of the 12 th Symposium on apatite, 6-7, Dec. 1996.
13.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu : Apatite forming method -duration of dentinal tubules occlusion-, Transactions of the 12th Symposium on apatite, 4-5, Dec. 1996.
14.
Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu : A new treatment method for dentin hypersensitivity -apatite forming method-, Transactions of the 12th Symposium on apatite, 2-3, Dec. 1996.
15.
Toshiyuki Suge, Akiko Kawasaki, 野杁 由一郎, 石川 邦夫 and 恵比須 繁之 : Treatment of dentin hypersensitivity by calcium phosphate precipitation method, --- occludion ability of CPP method in vivo ---, The Japanese Journal of Conservative Dentistry, Vol.39, 118, Mar. 1996.
16.
Toshiyuki Suge, 吉山 昌宏, Akiko Kawasaki and 恵比須 繁之 : Treatment of dentin hypersensitivity by calcium phosphate precipitation method, --- reevaluation of post-treatment solutions for clinical use ---, The Japanese Journal of Conservative Dentistry, Vol.38, 160, Oct. 1995.
17.
Akiko Kawasaki, Toshiyuki Suge, 清水 洋利, 吉山 昌宏 and 恵比須 繁之 : Effects of plaque control on the root hypersensitivity, The Japanese Journal of Conservative Dentistry, Vol.38, 17, Jun. 1995.
18.
Akiko Kawasaki, 石川 邦夫, Toshiyuki Suge, 吉山 昌宏 and 恵比須 繁之 : Effects of calcium fluorosilicate on the occlusion of dentinal tubules with calcium-phosphate precipitation method, The Japanese Journal of Conservative Dentistry, Vol.37, 69, Nov. 1994.
19.
Toshiyuki Suge, 石川 邦夫, Akiko Kawasaki, 吉山 昌宏 and 恵比須 繁之 : Treatment of dentin hypersensitivity by apatite forming method, --- duration of dentinal tubule occlusion ---, The Japanese Journal of Conservative Dentistry, Vol.37, 67, May 1994.
20.
吉山 昌宏, Toshiyuki Suge, Akiko Kawasaki and 恵比須 繁之 : プライマー処理による歯頚部磨耗象牙質の微細形態学的変化, The Japanese Journal of Conservative Dentistry, Vol.36, 131, Nov. 1993.
21.
Akiko Kawasaki, Toshiyuki Suge, 吉山 昌宏 and 恵比須 繁之 : 知覚過敏を発症した歯根面象牙質の超微構造, The Japanese Journal of Conservative Dentistry, Vol.36, 168, Nov. 1993.
22.
Toshiyuki Suge, 大木元 玲子, Akiko Kawasaki, 吉山 昌宏 and 恵比須 繁之 : アパタイト析出法を利用した象牙質知覚過敏症の治療法, The Japanese Journal of Conservative Dentistry, Vol.36, 23, Nov. 1993.
23.
Toshiyuki Suge, Akiko Kawasaki, 吉山 昌宏, 恵比須 繁之 and 石川 邦夫 : 象牙質知覚過敏症の治療法, The Japanese Journal of Conservative Dentistry, Vol.36, 44, Jun. 1993.
24.
吉山 昌宏, Toshiyuki Suge, Akiko Kawasaki and 恵比須 繁之 : 象牙質知覚過敏症の発症機構における突起様構造物の役割, The Japanese Journal of Conservative Dentistry, Vol.35, 16, Nov. 1992.
25.
吉山 昌宏, Toshiyuki Suge and 恵比須 繁之 : 象牙質知覚過敏症の発症機構, The Japanese Journal of Conservative Dentistry, Vol.35, 27, Jun. 1992.
26.
吉山 昌宏, Toshiyuki Suge, 白神 俊之, Kazumi Ozaki and 恵比須 繁之 : 象牙質知覚過敏症の発症機構, 硬組織研究技術談話会, Mar. 1992.
Development of a new approach to the treatments in conservative dentistry based on a concept for home or visit care. (Project/Area Number: 17K11708 )
Development of the method for prevention of root caries with antibacterial ammonium hexafluorosilicate (Project/Area Number: 16K11553 )
Development of the method for prevention of dental caries with antibacterial ammonium hexafluorosilicate (Project/Area Number: 25462961 )
Development of a new comprehensive treatment for dental caries based on minimum intervention dentistry (Project/Area Number: 23390435 )
Development of the method for prevention of dental caries with antibacterial ammonium hexafluorosilicate (Project/Area Number: 22592121 )
Development of the diagnosis and treatment of root caries on its pathosis and pathogenesis (Project/Area Number: 20592229 )
Study of quorum-sensing system of invading bacteria in dentinal caries (Project/Area Number: 19592204 )
Development of treatment method for the prevention of dental caries and dentin hypersensitivity with ammonium hexafluorosilicate (Project/Area Number: 19592203 )
Analysis of the mechanisms that claudins expressed in periodontal diseased epithelium regulate the permeability of gingival epithelium (Project/Area Number: 18592264 )
Development of treatment method for prevention of dental caries and dentin hypersensitivity with silica-fluoride compound (Project/Area Number: 16591916 )
Treatment of dentin hypersensitivity and dental caries by ammonium hexafluorosilicate (Project/Area Number: 13672002 )
Study on the role of chemokines in the infiltration of inflammatory cells in dental pulp tissue destruction (Project/Area Number: 13672001 )
Analysis of the relationship between the invasion of dental caries-associated bacteria and the host response of dental pulp in human carious teeth with pulp exposure (Project/Area Number: 11671897 )