徳島大学 / 教育研究者総覧 --- 石丸善康

個人のホームページ

https://researchmap.jp/ishimaruyoshiyasu (researchmap)

専門分野

○	発生生物学 (Developmental Biology), 分子生物学 (Molecular Biology)

研究テーマ

○	発生・再生のメカニズム

著書・論文

著書:

1.	石丸善康, 伊藤伸一, 宇野剛史, 岡村康弘, 大平健司, 小野公輔, 掛井秀一, 金西計英, 澤田麻衣子, 蓮沼徹, 古屋 S. 玲, 松浦健二, 松本和幸, 土岐順子, 阿部香織, 山﨑紅, 辰巳丈夫, 勝村幸博 : 情報科学入門, 日経BP社, 東京, 2019年4月.
2.	石丸善康, 伊藤伸一, 宇野剛史, 岡村康弘, 大平健司, 小野公輔, 掛井秀一, 金西計英, 澤田麻衣子, 蓮沼徹, 古屋 S. 玲, 松浦健二, 松本和幸, 土岐順子, 阿部香織, 山﨑紅, 辰巳丈夫, 勝村幸博 : 情報科学入門, 日経BP社, 東京, 2018年4月.
3.	石丸善康, 伊藤伸一, 宇野剛史, 岡村康弘, 大平健司, 小野公輔, 掛井秀一, 金西計英, 澤田麻衣子, 蓮沼徹, 古屋 S. 玲, 松浦健二, 松本和幸, 土岐順子, 阿部香織, 山﨑紅, 辰巳丈夫, 勝村幸博 : 情報科学入門, 日経BP社, 東京, 2017年4月.

学術論文(審査論文):

1.	Takahisa Yamashita, Takahiro Ohde, Taro Nakamura, Yoshiyasu Ishimaru, Takahito Watanabe, Sayuri Tomonari, Yuki Nakamura, Sumihare Noji and Taro Mito : Involvement of the scalloped gene in morphogenesis of the wing margin via regulating cell growth in a hemimetabolous insect Gryllus bimaculatus., Development Growth & Differentiation, Vol.65, No.6, 348-359, 2023. (要約) The acquisition of wings was a key event in insect evolution. As hemimetabolous insects were the first group to acquire functional wings, establishing the mechanisms of wing formation in this group could provide useful insights into their evolution. In this study, we aimed to elucidate the expression and function of the gene scalloped (sd), which is involved in wing formation in Drosophila melanogaster, and in Gryllus bimaculatus mainly during postembryonic development. Expression analysis showed that sd is expressed in the tergal edge, legs, antennae, labrum, and cerci during embryogenesis and in the distal margin of the wing pads from at least the sixth instar in the mid to late stages. Because sd knockout caused early lethality, nymphal RNA interference experiments were performed. Malformations were observed in the wings, ovipositor, and antennae. By analyzing the effects on wing morphology, it was revealed that sd is mainly involved in the formation of the margin, possibly through the regulation of cell proliferation. In conclusion, sd might regulate the local growth of wing pads and influence wing margin morphology in Gryllus. (キーワード) Animals / Cell Cycle / Cell Proliferation / Embryonic Development / Insect Proteins / Wings, Animal / Gryllidae / Transcription Factors (出版サイトへのリンク) ● Publication site (DOI): 10.1111/dgd.12869 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37310211 ● Summary page in Scopus @ Elsevier: 2-s2.0-85162895470 (DOI: 10.1111/dgd.12869, PubMed: 37310211, Elsevier: Scopus)
2.	Shintaro Inoue, Takahito Watanabe, Taiki Hamaguchi, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito : Combinatorial expression of ebony and tan generates body color variation from nymph through adult stages in the cricket, Gryllus bimaculatus., PLoS ONE, Vol.18, No.5, 2023. (要約) Insect body colors and patterns change markedly during development in some species as they adapt to their surroundings. The contribution of melanin and sclerotin pigments, both of which are synthesized from dopamine, to cuticle tanning has been well studied. Nevertheless, little is known about how insects alter their body color patterns. To investigate this mechanism, the cricket Gryllus bimaculatus, whose body color patterns change during postembryonic development, was used as a model in this study. We focused on the ebony and tan genes, which encode enzymes that catalyze the synthesis and degradation, respectively, of the precursor of yellow sclerotin N-β-alanyl dopamine (NBAD). Expression of the G. bimaculatus (Gb) ebony and tan transcripts tended to be elevated just after hatching and the molting period. We found that dynamic alterations in the combined expression levels of Gb'ebony and Gb'tan correlated with the body color transition from the nymphal stages to the adult. The body color of Gb'ebony knockout mutants generated by CRISPR/Cas9 systemically darkened. Meanwhile, Gb'tan knockout mutants displayed a yellow color in certain areas and stages. The phenotypes of the Gb'ebony and Gb'tan mutants probably result from an over-production of melanin and yellow sclerotin NBAD, respectively. Overall, stage-specific body color patterns in the postembryonic stages of the cricket are governed by the combinatorial expression of Gb'ebony and Gb'tan. Our findings provide insights into the mechanism by which insects evolve adaptive body coloration at each developmental stage. (キーワード) Animals / Melanins / Gryllidae / Nymph / ドーパミン (dopamine) / Insect Proteins (徳島大学機関リポジトリ) ● Metadata: 118982 (出版サイトへのリンク) ● Publication site (DOI): 10.1371/journal.pone.0285934 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37200362 ● Search Scopus @ Elsevier (PMID): 37200362 ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0285934 (徳島大学機関リポジトリ: 118982, DOI: 10.1371/journal.pone.0285934, PubMed: 37200362)
3.	Yuki Nakamura, Sayuri Tomonari, Kohei Kawamoto, Takahisa Yamashita, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Evolutionarily conserved function of the even-skipped ortholog in insects revealed by gene knock-out analyses in Gryllus bimaculatus., Developmental Biology, Vol.485, 1-8, 2022. (要約) Comparing the developmental mechanisms of segmentation among insects with different modes of embryogenesis provides insights on how the function of segmentation genes evolved. Functional analysis of eve by genetic mutants shows that the Drosophila pair-rule gene, even-skipped (eve), contributes to initial segmental patterning. However, eve orthologs tends to have diverse functions in other insects. To compare the evolutionary functional divergence of this gene, we evaluated eve function in a phylogenetically basal insect, the cricket Gryllus bimaculatus. To investigate the phenotypic effects of eve gene knock-out, we generated CRISPR/Cas9 system-mediated mutant strains of the cricket. CRISPR/Cas9 mutagenesis of multiple independent sites in the eve coding region revealed that eve null mutant embryos were defective in forming the gnathal, thoracic, and abdominal segments, consequently shortening the anterior-posterior axis. In contrast, the structures of the anterior and posterior ends (e.g., antenna, labrum, and cercus) formed normally. Hox gene expression in the gnathal, thoracic, and abdominal segments was detected in the mutant embryos. Overall, this study showed that Gryllus eve plays an important role in embryonic elongation and the formation of segmental boundaries in the gnathal to abdominal region of crickets. In the light of studies on other species, the eve function shown in Gryllus might be ancestral in insects. (キーワード) Amino Acid Sequence / Animals / Body Patterning / Drosophila / Drosophila Proteins / Gene Expression Regulation, Developmental / Gryllidae / Homeodomain Proteins / Insecta / RNA Interference / Transcription Factors (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.ydbio.2022.02.005 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35196518 ● Search Scopus @ Elsevier (PMID): 35196518 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2022.02.005 (DOI: 10.1016/j.ydbio.2022.02.005, PubMed: 35196518)
4.	Tetsuya Bando, Misa Okumura, Yuki Bando, Marou Hagiwara, Yoshimasa Hamada, Yoshiyasu Ishimaru, Taro Mito, Eri Kawaguchi, Takeshi Inoue, Kiyokazu Agata, Sumihare Noji and Hideyo Ohuchi : Toll signalling promotes blastema cell proliferation during cricket leg regeneration via insect macrophages., Development, Vol.149, No.8, 2022. (要約) Hemimetabolous insects, such as the two-spotted cricket Gryllus bimaculatus, can recover lost tissues, in contrast to the limited regenerative abilities of human tissues. Following cricket leg amputation, the wound surface is covered by the wound epidermis, and plasmatocytes, which are insect macrophages, accumulate in the wound region. Here, we studied the function of Toll-related molecules identified by comparative RNA sequencing during leg regeneration. Of the 11 Toll genes in the Gryllus genome, expression of Toll2-1, Toll2-2 and Toll2-5 was upregulated during regeneration. RNA interference (RNAi) of Toll, Toll2-1, Toll2-2, Toll2-3 or Toll2-4 produced regeneration defects in more than 50% of crickets. RNAi of Toll2-2 led to a decrease in the ratio of S- and M-phase cells, reduced expression of JAK/STAT signalling genes, and reduced accumulation of plasmatocytes in the blastema. Depletion of plasmatocytes in crickets using clodronate also produced regeneration defects, as well as fewer proliferating cells in the regenerating legs. Plasmatocyte depletion also downregulated the expression of Toll and JAK/STAT signalling genes in the regenerating legs. These results suggest that Spz-Toll-related signalling in plasmatocytes promotes leg regeneration through blastema cell proliferation by regulating the Upd-JAK/STAT signalling pathway. (キーワード) Animals / Gene Expression Regulation / Gryllidae / Hindlimb / Insect Proteins / Regeneration / Signal Transduction / Toll-Like Receptors (出版サイトへのリンク) ● Publication site (DOI): 10.1242/dev.199916 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34622924 ● Search Scopus @ Elsevier (PMID): 34622924 ● Search Scopus @ Elsevier (DOI): 10.1242/dev.199916 (DOI: 10.1242/dev.199916, PubMed: 34622924)
5.	Guillem Ylla, Taro Nakamura, Takehiko Itoh, Rei Kajitani, Atsushi Toyoda, Sayuri Tomonari, Tetsuya Bando, Yoshiyasu Ishimaru, Takahito Watanabe, Masao Fuketa, Yuji Matsuoka, A Austen Barnett, Sumihare Noji, Taro Mito and G Cassandra Extavour : Insights into the genomic evolution of insects from cricket genomes., Communications Biology, Vol.4, No.1, 2021. (要約) Most of our knowledge of insect genomes comes from Holometabolous species, which undergo complete metamorphosis and have genomes typically under 2 Gb with little signs of DNA methylation. In contrast, Hemimetabolous insects undergo the presumed ancestral process of incomplete metamorphosis, and have larger genomes with high levels of DNA methylation. Hemimetabolous species from the Orthopteran order (grasshoppers and crickets) have some of the largest known insect genomes. What drives the evolution of these unusual insect genome sizes, remains unknown. Here we report the sequencing, assembly and annotation of the 1.66-Gb genome of the Mediterranean field cricket Gryllus bimaculatus, and the annotation of the 1.60-Gb genome of the Hawaiian cricket Laupala kohalensis. We compare these two cricket genomes with those of 14 additional insects and find evidence that hemimetabolous genomes expanded due to transposable element activity. Based on the ratio of observed to expected CpG sites, we find higher conservation and stronger purifying selection of methylated genes than non-methylated genes. Finally, our analysis suggests an expansion of the pickpocket class V gene family in crickets, which we speculate might play a role in the evolution of cricket courtship, including their characteristic chirping. (キーワード) Animals / DNA Methylation / DNA Transposable Elements / Evolution, Molecular / Female / Genes, Insect / Genome, Insect / Gryllidae / Insecta / Male / Phylogeny / Repetitive Sequences, Nucleic Acid / Sequence Analysis, DNA (徳島大学機関リポジトリ) ● Metadata: 116893 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s42003-021-02197-9 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34127782 ● Search Scopus @ Elsevier (PMID): 34127782 ● Search Scopus @ Elsevier (DOI): 10.1038/s42003-021-02197-9 (徳島大学機関リポジトリ: 116893, DOI: 10.1038/s42003-021-02197-9, PubMed: 34127782)
6.	Yoshiyasu Ishimaru, Sayuri Tomonari, Takahito Watanabe, Sumihare Noji and Taro Mito : Regulatory mechanisms underlying the specification of the pupal-homologous stage in a hemimetabolous insect, Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, Vol.374, No.1783, 20190225, 2019. (要約) Juvenile hormones and the genetic interaction between the transcription factors Krüppel homologue 1 (Kr-h1) and Broad (Br) regulate the transformation of insects from immature to adult forms in both types of metamorphosis (holometaboly with a pupal stage versus hemimetaboly with no pupal stage); however, knowledge about the exact instar in which this occurs is limited. Using the hemimetabolous cricket Gryllus bimaculatus (Gb), we demonstrate that a genetic interaction occurs among Gb'Kr-h1, Gb'Br and the adult-specifier transcription factor Gb'E93 from the sixth to final (eighth) nymphal instar. Gb'Kr-h1 and Gb'Br mRNAs were strongly expressed in the abdominal tissues of sixth instar nymphs, with precocious adult moults being induced by Gb'Kr-h1 or Gb'Br knockdown in the sixth instar. The depletion of Gb'Kr-h1 or Gb'Br upregulates Gb'E93 in the sixth instar. By contrast, Gb'E93 knockdown at the sixth instar prevents nymphs transitioning to adults, instead producing supernumerary nymphs. Gb'E93 also represses Gb'Kr-h1 and Gb'Br expression in the penultimate nymphal instar, demonstrating its important role in adult differentiation. Our results suggest that the regulatory mechanisms underlying the pupal transition in holometabolous insects are evolutionarily conserved in hemimetabolous G. bimaculatus, with the penultimate and final nymphal periods being equivalent to the pupal stage. This article is part of the theme issue 'The evolution of complete metamorphosis'. (徳島大学機関リポジトリ) ● Metadata: 113697 (出版サイトへのリンク) ● Publication site (DOI): 10.1098/rstb.2019.0225 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31438810 ● Search Scopus @ Elsevier (PMID): 31438810 ● Search Scopus @ Elsevier (DOI): 10.1098/rstb.2019.0225 (徳島大学機関リポジトリ: 113697, DOI: 10.1098/rstb.2019.0225, PubMed: 31438810)
7.	Yoshiyasu Ishimaru, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji and Taro Mito : Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus, Development Growth & Differentiation, Vol.60, No.6, 377-386, 2018. (要約) The cricket, Gryllus bimaculatus, is a classic model of leg regeneration following amputation. We previously demonstrated that Gryllus decapentaplegic (Gb'dpp) is expressed during leg regeneration, although it remains unclear whether it is essential for this process. In this study, double-stranded RNA targeting the Smad mathers-against-dpp homolog, Gb'mad, was used to examine the role of bone morphogenetic protein (BMP) signaling in the leg regeneration process of Gryllus bimaculatus. RNA interference (RNAi)-mediated knockdown of Gb'mad led to a loss of tarsus regeneration at the most distal region of regenerating leg segments. Moreover, we confirmed that the phenotype obtained by knockdown of Dpp type I receptor, Thick veins (Gb'tkv), closely resembled that observed for Gb'mad RNAi crickets, thereby suggesting that the BMP signaling pathway is indispensable for the initial stages of tarsus formation. Interestingly, knockdown of Gb'mad and Gb'tkv resulted in significant elongation of regenerating tibia along the proximodistal axis compared with normal legs. Moreover, our findings indicate that during the regeneration of tibia, the BMP signaling pathway interacts with Dachsous/Fat (Gb'Ds/Gb'Ft) signaling and dachshund (Gb'dac) to re-establish positional information and regulate determination of leg size. Based on these observations, we discuss possible roles for Gb'mad in the distal patterning and intercalation processes during leg regeneration in Gryllus bimaculatus. (徳島大学機関リポジトリ) ● Metadata: 116416 (出版サイトへのリンク) ● Publication site (DOI): 10.1111/dgd.12560 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30043459 ● Search Scopus @ Elsevier (PMID): 30043459 ● Search Scopus @ Elsevier (DOI): 10.1111/dgd.12560 (徳島大学機関リポジトリ: 116416, DOI: 10.1111/dgd.12560, PubMed: 30043459)
8.	Hiroko Awata, Ryo Wakuda, Yoshiyasu Ishimaru, Yuji Matsuoka, Kanta Terao, Satomi Katata, Yukihisa Matsumoto, Yoshitaka Hamanaka, Sumihare Noji, Taro Mito and Makoto Mizunami : Roles of OA1 octopamine receptor and Dop1 dopamine receptor in mediating appetitive and aversive reinforcement revealed by RNAi studies., Scientific Reports, Vol.6, 29696, 2016. (要約) Revealing reinforcing mechanisms in associative learning is important for elucidation of brain mechanisms of behavior. In mammals, dopamine neurons are thought to mediate both appetitive and aversive reinforcement signals. Studies using transgenic fruit-flies suggested that dopamine neurons mediate both appetitive and aversive reinforcements, through the Dop1 dopamine receptor, but our studies using octopamine and dopamine receptor antagonists and using Dop1 knockout crickets suggested that octopamine neurons mediate appetitive reinforcement and dopamine neurons mediate aversive reinforcement in associative learning in crickets. To fully resolve this issue, we examined the effects of silencing of expression of genes that code the OA1 octopamine receptor and Dop1 and Dop2 dopamine receptors by RNAi in crickets. OA1-silenced crickets exhibited impairment in appetitive learning with water but not in aversive learning with sodium chloride solution, while Dop1-silenced crickets exhibited impairment in aversive learning but not in appetitive learning. Dop2-silenced crickets showed normal scores in both appetitive learning and aversive learning. The results indicate that octopamine neurons mediate appetitive reinforcement via OA1 and that dopamine neurons mediate aversive reinforcement via Dop1 in crickets, providing decisive evidence that neurotransmitters and receptors that mediate appetitive reinforcement indeed differ among different species of insects. (徳島大学機関リポジトリ) ● Metadata: 112322 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/srep29696 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27412401 ● Summary page in Scopus @ Elsevier: 2-s2.0-84978655727 (徳島大学機関リポジトリ: 112322, DOI: 10.1038/srep29696, PubMed: 27412401, Elsevier: Scopus)
9.	Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji and Taro Mito : TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis., Proceedings of the National Academy of Sciences of the United States of America, Vol.113, No.20, 5634-5639, 2016. (要約) Although butterflies undergo a dramatic morphological transformation from larva to adult via a pupal stage (holometamorphosis), crickets undergo a metamorphosis from nymph to adult without formation of a pupa (hemimetamorphosis). Despite these differences, both processes are regulated by common mechanisms that involve 20-hydroxyecdysone (20E) and juvenile hormone (JH). JH regulates many aspects of insect physiology, such as development, reproduction, diapause, and metamorphosis. Consequently, strict regulation of JH levels is crucial throughout an insect's life cycle. However, it remains unclear how JH synthesis is regulated. Here, we report that in the corpora allata of the cricket, Gryllus bimaculatus, Myoglianin (Gb'Myo), a homolog of Drosophila Myoglianin/vertebrate GDF8/11, is involved in the down-regulation of JH production by suppressing the expression of a gene encoding JH acid O-methyltransferase, Gb'jhamt In contrast, JH production is up-regulated by Decapentaplegic (Gb'Dpp) and Glass-bottom boat/60A (Gb'Gbb) signaling that occurs as part of the transcriptional activation of Gb'jhamt Gb'Myo defines the nature of each developmental transition by regulating JH titer and the interactions between JH and 20E. When Gb'myo expression is suppressed, the activation of Gb'jhamt expression and secretion of 20E induce molting, thereby leading to the next instar before the last nymphal instar. Conversely, high Gb'myo expression induces metamorphosis during the last nymphal instar through the cessation of JH synthesis. Gb'myo also regulates final insect size. Because Myo/GDF8/11 and Dpp/bone morphogenetic protein (BMP)2/4-Gbb/BMP5-8 are conserved in both invertebrates and vertebrates, the present findings provide common regulatory mechanisms for endocrine control of animal development. (徳島大学機関リポジトリ) ● Metadata: 116415 (出版サイトへのリンク) ● Publication site (DOI): 10.1073/pnas.1600612113 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27140602 ● Summary page in Scopus @ Elsevier: 2-s2.0-84969765307 (徳島大学機関リポジトリ: 116415, DOI: 10.1073/pnas.1600612113, PubMed: 27140602, Elsevier: Scopus)
10.	Yoshimasa Hamada, Tetsuya Bando, Taro Nakamura, Yoshiyasu Ishimaru, Taro Mito, Sumihare Noji, Kenji Tomioka and Hideyo Ohuchi : Regenerated leg segment patterns are regulated epigenetically by histone H3K27 methylation in the cricket Gryllus bimaculatus, Development, Vol.142, No.17, 2916-2927, 2015. (要約) Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)(RNAi) and was increased by Gb'Utx(RNAi). Regenerated Gb'E(z)(RNAi) cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'Utx(RNAi) cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)(RNAi) regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'Utx(RNAi) regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression. (徳島大学機関リポジトリ) ● Metadata: 115797 (出版サイトへのリンク) ● Publication site (DOI): 10.1242/dev.122598 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26253405 ● Summary page in Scopus @ Elsevier: 2-s2.0-84940734372 (徳島大学機関リポジトリ: 115797, DOI: 10.1242/dev.122598, PubMed: 26253405, Elsevier: Scopus)
11.	Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadic and Taro Mito : Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes., Biology Open, Vol.4, No.6, 702-709, 2015. (要約) In insect species that undergo long germ segmentation, such as Drosophila, all segments are specified simultaneously at the early blastoderm stage. As embryogenesis progresses, the expression boundaries of Hox genes are established by repression of gap genes, which is subsequently replaced by Polycomb group (PcG) silencing. At present, however, it is not known whether patterning occurs this way in a more ancestral (short germ) mode of embryogenesis, where segments are added gradually during posterior elongation. In this study, two members of the PcG family, Enhancer of zeste (E(z)) and Suppressor of zeste 12 (Su(z)12), were analyzed in the short germ cricket, Gryllus bimaculatus. Results suggest that although stepwise negative regulation by gap and PcG genes is present in anterior members of the Hox cluster, it does not account for regulation of two posterior Hox genes, abdominal-A (abd-A) and Abdominal-B (Abd-B). Instead, abd-A and Abd-B are predominantly regulated by PcG genes, which is the mode present in vertebrates. These findings suggest that an intriguing transition of the PcG-mediated silencing of Hox genes may have occurred during animal evolution. The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains. Then, during insect evolution, the repression by transcription factors may have been acquired in anterior Hox genes of short germ insects, while PcG silencing was maintained in posterior Hox genes. (徳島大学機関リポジトリ) ● Metadata: 114742 (出版サイトへのリンク) ● Publication site (DOI): 10.1242/bio.201411064 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25948756 ● Search Scopus @ Elsevier (PMID): 25948756 ● Search Scopus @ Elsevier (DOI): 10.1242/bio.201411064 (徳島大学機関リポジトリ: 114742, DOI: 10.1242/bio.201411064, PubMed: 25948756)
12.	Yoshiyasu Ishimaru, Taro Nakamura, Tetsuya Bando, Yuji Matsuoka, Hideyo Ohuchi, Sumihare Noji and Taro Mito : Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration., Scientific Reports, Vol.5, 8387, 2015. (要約) Cricket nymphs have the remarkable ability to regenerate a functional leg following amputation, indicating that the regenerating blastemal cells contain information for leg morphology. However, the molecular mechanisms that underlie regeneration of leg patterns remain poorly understood. Here, we analyzed phenotypes of the tibia and tarsus (three tarsomeres) obtained by knockdown with regeneration-dependent RNA interference (rdRNAi) against Gryllus dachshund (Gb'dac) and Distal-less (Gb'Dll). We found that depletion of Gb'Dll mRNA results in loss of the tarsal segments, while rdRNAi against Gb'dac shortens the tibia at the two most distal tarsomeres. These results indicate that Gb'Dll expression is indispensable for formation of the tarsus, while Gb'dac expression is necessary for elongation of the tibia and formation of the most proximal tarsomere. These findings demonstrate that mutual transcriptional regulation between the two is indispensable for formation of the tarsomeres, whereas Gb'dac is involved in determination of tibial size through interaction with Gb'ds/Gb'ft. (徳島大学機関リポジトリ) ● Metadata: 114975 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/srep08387 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25669615 ● Summary page in Scopus @ Elsevier: 2-s2.0-84922949748 (徳島大学機関リポジトリ: 114975, DOI: 10.1038/srep08387, PubMed: 25669615, Elsevier: Scopus)
13.	Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji and Taro Mito : Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus, Development, Vol.140, No.5, 959-964, 2013. (要約) In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'dome(RNAi), Gb'hop(RNAi) and Gb'Stat(RNAi) crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'Stat(RNAi) cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fat(RNAi) or Gb'warts(RNAi) was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration. (キーワード) Animals / Cell Proliferation / Gene Expression Profiling / Gryllidae / Janus Kinases / Lower Extremity / RNA (RNA) / 再生 (regeneration) / STAT Transcription Factors / Sequence Analysis, RNA / Statistics as Topic / Transcriptome / Validation Studies as Topic (出版サイトへのリンク) ● Publication site (DOI): 10.1242/dev.084590 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23344706 ● Summary page in Scopus @ Elsevier: 2-s2.0-84873739376 (DOI: 10.1242/dev.084590, PubMed: 23344706, Elsevier: Scopus)
14.	Yoshiyasu Ishimaru, Tomoko Komatsu, Megumi Kasahara, Yuko Katoh-Fukui, Hidesato Ogawa, Yoshiro Toyama, Mamiko Maekawa, Kiyotaka Toshimori, S Roshantha A Chandraratna, Ken-ichirou Morohashi and Hidefumi Yoshioka : Mechanism of asymmetric ovarian development in chick embryos., Development, Vol.135, No.4, 677-685, 2008. (要約) In most animals, the gonads develop symmetrically, but most birds develop only a left ovary. A possible role for estrogen in this asymmetric ovarian development has been proposed in the chick, but the mechanism underlying this process is largely unknown. Here, we identify the molecular mechanism responsible for this ovarian asymmetry. Asymmetric PITX2 expression in the left presumptive gonad leads to the asymmetric expression of the retinoic-acid (RA)-synthesizing enzyme, RALDH2, in the right presumptive gonad. Subsequently, RA suppresses expression of the nuclear receptors Ad4BP/SF-1 and estrogen receptor alpha in the right ovarian primordium. Ad4BP/SF-1 expressed in the left ovarian primordium asymmetrically upregulates cyclin D1 to stimulate cell proliferation. These data suggest that early asymmetric expression of PITX2 leads to asymmetric ovarian development through up- or downregulation of RALDH2, Ad4BP/SF-1, estrogen receptor alpha and cyclin D1. (キーワード) Animals / Body Patterning / Cell Proliferation / Chick Embryo / Cyclin D1 / Cytochrome P-450 Enzyme System / Estrogen Receptor alpha / Female / Gene Expression Regulation, Developmental / Homeodomain Proteins / Models, Biological / Ovary / Sex Determination Processes / Signal Transduction / Steroidogenic Factor 1 / Transcription Factors / Tretinoin (出版サイトへのリンク) ● Publication site (DOI): 10.1242/dev.012856 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18199582 ● Search Scopus @ Elsevier (PMID): 18199582 ● Search Scopus @ Elsevier (DOI): 10.1242/dev.012856 (DOI: 10.1242/dev.012856, PubMed: 18199582)
15.	Hidefumi Yoshioka, Yoshiyasu Ishimaru, Noriyuki Sugiyama, Naoki Tsunekawa, Toshiaki Noce, Megumi Kasahara and Ken-ichirou Morohashi : Mesonephric FGF signaling is associated with the development of sexually indifferent gonadal primordium in chick embryos., Developmental Biology, Vol.280, No.1, 150-161, 2005. (要約) The gonad as well as the reproductive tracts, kidney, and adrenal cortex are derived from the intermediate mesoderm. In addition, the intermediate mesoderm forms the mesonephros. Although the mesonephros is the source of certain testicular cell types, its contribution to gonad formation through expression of growth factors is largely unknown. Here, we examined the expression profiles of FGF9 in the developing mesonephros of chick embryos at sexually indifferent stages, and found that the expression domain is adjacent to the gonadal primordium. Moreover, FGFR3 (FGF receptor 3) showed a strong expression in the gonadal primordium. Next, we examined the functions of FGF signal during gonadal development with misexpressed FGF9. Interestingly, misexpression of FGF9 led to gonadal expansion through stimulation of cell proliferation. In contrast, treatment with a chemical inhibitor for FGFR decreased cell proliferation and resulted in reduction of the gonadal size. Simultaneously, the treatment resulted in reduction of gonadal marker gene expression. Our study demonstrated that FGF expressed in the developing mesonephros is involved in the development of the gonad at the sexually indifferent stages through stimulation of gonadal cell proliferation and gonadal marker gene expression. (キーワード) Animals / Biological Markers / Cell Proliferation / Chick Embryo / DNA-Binding Proteins / Fibroblast Growth Factor 9 / Fibroblast Growth Factors / Gene Expression Regulation, Developmental / Gonads / Homeodomain Proteins / In Situ Hybridization / Mesonephros / Mice / Protein-Tyrosine Kinases / Pyrroles / Receptor, Fibroblast Growth Factor, Type 3 / Receptors, Cytoplasmic and Nuclear / Receptors, Fibroblast Growth Factor / Sex Differentiation / Signal Transduction / Steroidogenic Factor 1 / Transcription Factors (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.ydbio.2005.01.011 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15766755 ● Search Scopus @ Elsevier (PMID): 15766755 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2005.01.011 (DOI: 10.1016/j.ydbio.2005.01.011, PubMed: 15766755)
16.	Yoshioka Hidefumi, Yoshiyasu Ishimaru, Sugiyama Noriyuki, Kasahara Megumi and Morohashi Ken-ichirou : Mesonephric Wnt signaling associate with a formation of an adreno-gonadal primordium in chick embryos, Endocrine Research, Vol.30, No.4, 523, 2004.
17.	Morohashi Ken-ichirou, Yoshiyasu Ishimaru, Sugiyama Noriyuki and Yoshioka Hidefumi : Growth factors from mesonephros implicated in gonadal and adrenal differentiation, Endocrine Research, Vol.30, No.4, 511, 2004.
18.	Yoshiyasu Ishimaru, Hidefumi Yoshioka, Hirotaka Tao, Bernard Thisse, Christine Thisse, Wright V E Christopher, Hiroshi Hamada, Hideyo Ohuchi and Sumihare Noji : Asymmetric expression of antivin/lefty1 in the early chick embryo, Mechanisms of Development, Vol.90, No.1, 115-118, 2000. (要約) Mammalian lefty and zebrafish antivin, highly related to lefty, are shown to be expressed asymmetrically and involved in the specification of the left body side of early embryos. We isolated a chick homologue of the antivin/lefty1 cDNA and studied its expression pattern during early chick development. We found that antivin/lefty1 is expressed asymmetrically on the left side of the prospective floorplate, notochord and lateral plate mesoderm of the chick embryo. (キーワード) Amino Acid Sequence / Animals / Cell Polarity / Chick Embryo / Embryo, Nonmammalian / Gene Expression Regulation, Developmental / Left-Right Determination Factors / Molecular Sequence Data / Sequence Alignment / Transforming Growth Factor beta (出版サイトへのリンク) ● Publication site (DOI): 10.1016/S0925-4773(99)00232-4 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10585569 ● Summary page in Scopus @ Elsevier: 2-s2.0-0033990952 (DOI: 10.1016/S0925-4773(99)00232-4, PubMed: 10585569, Elsevier: Scopus)
19.	Hidefumi Yoshioka, Chikara Meno, Kazuko Koshiba, Minoru Sugihara, Hiroyuki Itoh, Yoshiyasu Ishimaru, Takashi Inoue, Hideyo Ohuchi, Elena V Semina, Jeffrey C Murray, Sumihare Noji and Hiroshi Hamada : Pitx2, a bicoid-type homeobox gene, is involved in a lefty-signaling pathway in determination of left-right asymmetry, Cell, Vol.94, No.3, 299-305, 1998. (要約) Signaling molecules such as Activin, Sonic hedgehog, Nodal, Lefty, and Vg1 have been found to be involved in determination of left-right (L-R) asymmetry in the chick, mouse, or frog. However, a common signaling pathway has not yet been identified in vertebrates. We report that Pitx2, a bicoid-type homeobox gene expressed asymmetrically in the left lateral plate mesoderm, may be involved in determination of L-R asymmetry in both mouse and chick. Since Pitx2 appears to be downstream of lefty-1 in the mouse pathway, we examined whether mouse Lefty proteins could affect the expression of Pitx2 in the chick. Our results indicate that a common pathway from lefty-1 to Pitx2 likely exists for determination of L-R asymmetry in vertebrates. (キーワード) Animals / Body Patterning / Chick Embryo / Embryo, Mammalian / Gene Expression Regulation, Developmental / Genes, Homeobox / Hedgehog Proteins / Homeodomain Proteins / Left-Right Determination Factors / Mice / Models, Genetic / Nuclear Proteins / Paired Box Transcription Factors / Protein Biosynthesis / Proteins / シグナル伝達 (signal transduction) / Trans-Activators / Transcription Factors / Transforming Growth Factor beta (出版サイトへのリンク) ● Publication site (DOI): 10.1016/S0092-8674(00)81473-7 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9708732 ● Summary page in Scopus @ Elsevier: 2-s2.0-0032493866 (DOI: 10.1016/S0092-8674(00)81473-7, PubMed: 9708732, Elsevier: Scopus)
20.	Hideyo Ohuchi, Jun Takeuchi, Hidefumi Yoshioka, Yoshiyasu Ishimaru, Keiko Ogura, Naoki Takahashi, Toshihiko Ogura and Sumihare Noji : Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4., Development, Vol.125, No.1, 51-60, 1998. (要約) It has been reported that members of the fibroblast growth factor (FGF) family can induce additional limb formation in the flank of chick embryos. The phenotype of the ectopic limb depends on the somite level at which it forms: limbs in the anterior flank resemble wings, whereas those in the posterior flank resemble legs. Ectopic limbs located in the mid-flank appear chimeric, possessing characteristics of both wings and legs; feather buds are present in the anterior halves with scales and claws in the posterior halves. To study the mechanisms underlying the chimerism of these additional limbs, we cloned chick Tbx5 and Tbx4 to use as forelimb and hindlimb markers and examined their expression patterns in FGF-induced limb buds. We found that Tbx5 and Tbx4 were differentially expressed in the anterior and posterior halves of additional limb buds in the mid-flank, respectively, consistent with the chimeric patterns of the integument. A boundary of Tbx5/Tbx4 exists in all ectopic limbs, indicating that the additional limbs are essentially chimeric, although the degree of chimerism is dependent on the position. The boundary of Tbx5/Tbx4 expression is not fixed at a specific position within the interlimb region, but dependent upon where FGF was applied. Since the ectopic expression patterns of Tbx5/Tbx4 in the additional limbs are closely correlated with the patterns of their chimeric phenotypes, it is likely that Tbx5 and Tbx4 expression in the limb bud is involved in determination of the forelimb and hindlimb identities, respectively, in vertebrates. (キーワード) Amino Acid Sequence / Animals / Avian Proteins / Body Patterning / Chick Embryo / Extremities / Fibroblast Growth Factor 2 / Fibroblast Growth Factor 4 / Fibroblast Growth Factor 8 / Fibroblast Growth Factors / Fibroblasts / Gene Expression Regulation, Developmental / Genetic Markers / Growth Substances / Humans / In Situ Hybridization / Molecular Sequence Data / Phenotype / Proto-Oncogene Proteins / RNA, Messenger / Recombinant Proteins / Sequence Alignment / T-Box Domain Proteins / Transcription Factors / Transfection / Wing (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9389663 ● Search Scopus @ Elsevier (PMID): 9389663 (PubMed: 9389663)
21.	Hideyo OhuchI, Takashi Nakagawa, Atsuyo Yamamoto, Akihiro Araga, Takeshi Ohata, Yoshiyasu Ishimaru, Hidefumi Yoshioka, Takashi Kuwana, Tsutomu Nohno, Masahiro Yamasaki, Nobuyuki Itoh and Sumihare Noji : The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor, Development, Vol.124, No.11, 2235-2244, 1997. (要約) Vertebrate limb formation has been known to be initiated by a factor(s) secreted from the lateral plate mesoderm. In this report, we provide evidence that a member of the fibroblast growth factor (FGF) family, FGF10, emanates from the prospective limb mesoderm to serve as an endogenous initiator for limb bud formation. Fgf10 expression in the prospective limb mesenchyme precedes Fgf8 expression in the nascent apical ectoderm. Ectopic application of FGF10 to the chick embryonic flank can induce Fgf8 expression in the adjacent ectoderm, resulting in the formation of an additional complete limb. Expression of Fgf10 persists in the mesenchyme of the established limb bud and appears to interact with Fgf8 in the apical ectoderm and Sonic hedgehog in the zone of polarizing activity. These results suggest that FGF10 is a key mesenchymal factor involved in the initial budding as well as the continuous outgrowth of vertebrate limbs. (キーワード) Amino Acid Sequence / Animals / Chick Embryo / Cloning, Molecular / Ectoderm / Fibroblast Growth Factor 10 / Fibroblast Growth Factor 8 / Fibroblast Growth Factors / Fibroblasts / Gene Expression Regulation, Developmental / Growth Substances / Hedgehog Proteins / Limb Buds / Mesoderm / Molecular Sequence Data / Proteins / RNA, Messenger / Trans-Activators (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9187149 ● CiNii @ 国立情報学研究所 (CRID): 1572261549162172288 ● Search Scopus @ Elsevier (PMID): 9187149 (PubMed: 9187149, CiNii: 1572261549162172288)
22.	Hidefumi Yoshioka, Hideyo Ohuchi, Yoshiyasu Ishimaru, Tetsuya Ishikawa, Tsutomu Nohno, Kaoru Saigo and Sumihare Noji : A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos, Development Growth & Differentiation, Vol.38, No.6, 617-624, 1996. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1520290882990130432 (CiNii: 1520290882990130432)

総説・解説:

1.	Taro Mito, Yoshiyasu Ishimaru, Takahito Watanabe, Taro Nakamura, Guillem Ylla, Sumihare Noji and G Cassandra Extavour : Cricket: The third domesticated insect., Current Topics in Developmental Biology, Vol.147, 291-306, Mar. 2022. (要約) Many researchers are using crickets to conduct research on various topics related to development and regeneration in addition to brain function, behavior, and biological clocks, using advanced functional and perturbational technologies such as genome editing. Recently, crickets have also been attracting attention as a food source for the next generation of humans. In addition, crickets are increasingly being used as disease models and biological factories for pharmaceuticals. Cricket research has thus evolved over the last century from use primarily in highly important basic research, to use in a variety of applications and practical uses. These insects are now a state-of-the-art model animal that can be obtained and maintained in large quantities at low cost. We therefore suggest that crickets are useful as a third domesticated insect for scientific research, after honeybees and silkworms, contributing to the achievement of global sustainable development goals. (キーワード) Animals / Bees / Gryllidae / Insecta (出版サイトへのリンク) ● Publication site (DOI): 10.1016/bs.ctdb.2022.02.003 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35337452 ● Search Scopus @ Elsevier (PMID): 35337452 ● Search Scopus @ Elsevier (DOI): 10.1016/bs.ctdb.2022.02.003 (DOI: 10.1016/bs.ctdb.2022.02.003, PubMed: 35337452)
2.	石丸善康, 野地澄晴, 三戸太郎 : 昆虫変態の分子機構:コオロギの研究から, 昆虫と自然, Vol.54, No.11, 38-41, 2019年10月. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1521136280023173888 (CiNii: 1521136280023173888)
3.	Tetsuya Bando, Taro Mito, Yoshimasa Hamada, Yoshiyasu Ishimaru, Sumihare Noji and Hideyo Ohuchi : Molecular mechanisms of limb regeneration: insights from regenerating legs of the cricket Gryllus bimaculatus, The International Journal of Developmental Biology, Vol.62, No.6-7-8, 559-569, Jun. 2018. (要約) This review summarizes recent advances in leg regeneration research, focusing on the cricket Gryllus bimaculatus. Recent studies have revealed molecular mechanisms on blastema formation, establishment of positional information, and epigenetic regulation during leg regeneration. Especially, these studies have provided molecular bases in classical conceptual models such as the polar coordinate model, the intercalation model, the boundary model, the steepness model, etc., which were proposed to interpret regeneration processes of the cockroach legs. When a leg is amputated, a blastema is formed through the activation of the Janus-kinase (Jak)/Signal-Transduction-and-Activator-of-Transcription (STAT) pathway. Subsequently, the Hedgehog/Wingless/Decapentaplegic/Epidermal-growth-factor pathways instruct distalization in the blastema, designated as the molecular boundary model. Downstream targets of this pathway are transcription factors Distal-less (Dll) and dachshund (dac), functioning as key regulators of proximodistal pattern formation. Dll and dac specify the distal and proximal regions in the blastema, respectively, through the regulation of tarsal patterning genes. The expression of leg patterning genes during regeneration may be epigenetically controlled by histone H3K27 methylation via Enhancer-of-zeste and Ubiquitously-transcribed-tetratricopeptide-repeat-gene-X-chromosome. For the molecular mechanism of intercalation of the missing structures between the amputated position and the most distal one, Dachsous/Fat (Ds/Ft) steepness model has been proposed, in which the Ds/Ft pathway maintains positional information and determines leg size through dac expression. This model was theoretically verified to interpret the experimental results obtained with cricket legs. Availability of whole-genome sequence information, regeneration-dependent RNA interference, and genome editing technique will have the cricket be an ideal model system to reveal gene functions in leg regeneration. (出版サイトへのリンク) ● Publication site (DOI): 10.1387/ijdb.180048ho (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29938767 ● Search Scopus @ Elsevier (PMID): 29938767 ● Search Scopus @ Elsevier (DOI): 10.1387/ijdb.180048ho (DOI: 10.1387/ijdb.180048ho, PubMed: 29938767)

国際会議:

1.	Shintaro Inoue, Takahito Watanabe, Hamaguchi Taiki, Fujie Kai, Shimamura Ayane, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito : Artificial modification of cricket body color: breeding for the next-generation of protein supply, International Conference of Non-Traditional Arthropod Model Systems, Aug. 2023.
2.	Fujie Kai, Shintaro Inoue, Hamaguchi Taiki, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji, Takahito Watanabe and Taro Mito : The discovery of two paralogous dopamine-synthase genes in the two-spotted cricket Gryllus bimaculatus, International Conference of Non-Traditional Arthropod Model Systems, Aug. 2023.
3.	Tetsuya Bando, Yoshiyasu Ishimaru, Hiroshi Yoshida, Taro Nakamura, Taro Mito, Hideyo Ohuchi and Sumihare Noji : Mechanisms underlying regeneration of the cricket leg, based on an extended steepness model, EMBO conference: The molecular and cellular basis of regeneration and tissue repair, Spain, Sep. 2014.
4.	Sumihare Noji, Taro Mito, Tetsuya Bando, Taro Nakamura, Takahito Watanabe, Yoshiyasu Ishimaru and Hideyo Ohuchi : Regeneration of insect legs from stem cells, Thirteenth International Congress on Invertebrate Reproduction and Development, USA, Jul. 2014.
5.	Sumihare Noji, Takahito Watanabe, Yoshiyasu Ishimaru, Taro Nakamura, Hiroshi Yoshida, Tetsuya Bando, Hideyo Ohuchi and Taro Mito : Regeneration of insect legs from stem cells Blastema formation to leg size determination, Invertebrate Reproduction and Development, USA, Jul. 2013.
6.	Hidefumi Yoshioka, Yoshiyasu Ishimaru, Tomoko Komatsu, Megumi Kasahara, Yuko Katoh-Fukui and Ken-ichirou Morohashi : Mechanism for asymmetric gonad development in birds, International Symposium on Molecular Mechanisms of Sex Determination and Differentiation at the 77th Annual Meeting of the Zoological Society of Japan, Matsue, Sep. 2006.
7.	Yoshiyasu Ishimaru : Mechanism of asymmetric ovarian development in the chicken, Fourth International Symposium on the Biology of Vertebrate Sex Determination, Hawaii, Apr. 2006.
8.	Ken-ichirou Morohashi, Yoshiyasu Ishimaru, Noriyuki Sugiyama and Hidefumi Yoshioka : Growth Factors from Mesonephros Implicated in Gonadal and Adrenal Differentiation, Eleventh conference on the Adrenal Cortex, USA, Jun. 2004.
9.	Hidefumi Yoshioka, Yoshiyasu Ishimaru, Noriyuki Sugiyama, Medumi Kasahara and Ken-ichirou Morohashi : Wnt Signaling Associates with a Formation of an Adreno-Gonadal Primordium in Chick Embryos, Eleventh conference on the Adrenal Cortex, USA, Jun. 2004.
10.	Ken-ichirou Morohashi, Masatomo Kusaka, Kanako Miyabayashi, Yoshiyasu Ishimaru, Mohamad Zubair, Hidesato Ogawa, Hidefumi Yoshioka and Yuko Katoh-Fukui : Transcriptional Regulation of Genes implicated in Gonad Development, Fourth International Symposium on the Biology of Vertebrate Sex Determination, Hawaii, USA, Apr. 2004.
11.	Hidefumi Yoshioka, Yoshiyasu Ishimaru, Noriyuki Sugiyama, Naoki Tsunekawa, Toshiaki Noce, Megumi Kasahara and Ken-ichirou Morohashi : FGF and WNT signaling required for the mesonephric tubulogenesis and the initiation of gonadal organogenesis, Third International Symposium on the Biology of Vertebrate Sex Determination, Hawaii, Apr. 2003.
12.	Hidefumi Yoshioka, Chikara Meno, Minoru Sugihara, Hiroyuki Itoh, Yoshiyasu Ishimaru, Kazuko Koshiba, Hideyo Ohuchi, Elena V. Semina, Jeffrey C. Murray, Hiroshi Hamada and Sumihare Noji : Pitx2, a bicoid type homeobox gene, is involved in a lefty-signaling pathway in determination of left-right asymmetry, CGGH symposium 98 " Signaling Systems Controlling Development, Nagoya, Oct. 1998.
13.	Hideyo Ohuchi, Hidefumi Yoshioka, Yoshiyasu Ishimaru, Hiroyuki Itoh, Nobuyuki Itoh and Sumihare Noji : Molecular mechanism for vertebrate limb initiation and specification, Keystone Symposia on molecular and cellular biology, USA, Apr. 1998.
14.	Hidefumi Yoshioka, Chikara Meno, Minoru Sugihara, Hiroyuki Itoh, Yoshiyasu Ishimaru, Kazuko Koshiba, Hideyo Ohuchi, Elena V. Semina, Jeffrey C. Murray, Hiroshi Hamada and Sumihare Noji : Rieg is involved in establishment of left-right asymmetry in both chick and mouse embryo, Keystone symposia on molecular and cellular biology, USA, Apr. 1998.
15.	Hidefumi Yoshioka, Hideyo Ohuchi, Yoshiyasu Ishimaru, Tetsuya Ishikawa, Tsutomu Nohno, Kaoru Saigo and Sumihare Noji : A Drosophila receptor type tyrosine kinase (DFR1) acts as an FGF receptor in Xenopus embryos, 12th International Symposium of Federation of Asian and Oceanian Biochemists and Molecular Biologists: Gene regulation of Biological Functions, Tokushima, Jul. 1996.

国内講演発表:

1.	岸伸旺, 渡辺崇人, 井上慎太郎, 濱口汰暉, 石丸善康, 宮脇克行, 髙橋章, 二川健, 野地澄晴, 三戸太郎 : フタホシコオロギにおけるクチクラ色素合成に関わる遺伝子の機能解析, 第68回応用動物昆虫学会, Vol.-, No.-, -, 2024年3月.
2.	井上慎太郎, 渡辺崇人, 藤江快, 島村彩音, 石丸善康, 宮脇克行, 髙橋章, 二川健, 三戸太郎 : コオロギをモデルとした昆虫の白色スクレロチン合成酵素遺伝子のメラニン生成制御機能の解析, 第68回応用動物昆虫学会, Vol.-, No.-, -, 2024年3月.
3.	NAMIKAWA Sayaka, Yoshiyasu Ishimaru, Sayuri Tomonari, Takahito Watanabe, Sumihare Noji and Taro Mito : フタホシコオロギにおける20-hydroxyecdysone(20E)合成に関わるBlimp-1遺伝子の機能解析, 第46回日本分子生物学会, Dec. 2023.
4.	井上慎太郎, 渡辺崇人, 濱口汰暉, 石丸善康, 宮脇克行, 二川健, 髙橋章, 野地澄晴, 三戸太郎 : コオロギをモデルとした体色パターン制御の分子メカニズムの解析, 第67回日本応用動物昆虫学会, 2023年3月.
5.	濱口汰暉, 井上慎太郎, 宮脇克行, 髙橋章, 二川健, 石丸善康, 野地澄晴, 渡辺崇人, 三戸太郎 : フタホシコオロギにおける色素合成に関わる遺伝子の機能解析, 第45回日本分子生物学会, 2022年12月.
6.	渡辺崇人, 井上慎太郎, 濱口汰暉, 石丸善康, 三戸太郎 : ゲノム編集を活用した食用コオロギの育種研究, 日本遺伝学会第94回大会, 2022年9月.
7.	井上慎太郎, 濱口汰暉, 石丸善康, 三戸太郎, 渡辺崇人 : フタホシコオロギの体色関連遺伝子のノックアウト系統作製および表現型解析, 第66回日本応用動物昆虫学会大会, 2022年3月.
8.	Hamaguchi Taiki, Shintaro Inoue, Takahito Watanabe, Yoshiyasu Ishimaru and Taro Mito : フタホシコオロギにおけるクチクラ形成と色素合成に関わる遺伝子の機能解析, The 44th Annual Meeting of the Molecular Biology Society of Japan, Dec. 2021.
9.	Higashihara Aya, Yoshiyasu Ishimaru, Matsumura Saki, Kawamoto Kohei, Sayuri Tomonari, Sumihare Noji and Taro Mito : Gene knock-out analysis of a metamorphosis factor Myoglianin in the cricket Gryllus bimaculatus, The 43rd Annual Meeting of the Molecular Biology Society of Japan, Online, Dec. 2020.
10.	山下貴久, 大出高弘, 友成さゆり, 中村雄軌, 石丸善康, 渡辺崇人, 三戸太郎 : フタホシコオロギの翅形成に関わる遺伝子の発現と機能解析, 第91回日本動物学会, 2020年9月.
11.	野地澄晴, 渡辺崇人, 石丸善康, 三戸太郎, 岡部慎司 : コオロギ(昆虫)を用いた宇宙食, 第63回宇宙科学技術連合講演会, 2019年11月.
12.	Takuya Watari, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Involvement of macrophages in leg regeneration of the cricket Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
13.	Kohei Kawamoto, Mayuko Matsuda, Takahisa Yamashita, Takahito Watanabe, Sayuri Tomonari, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Precise in-frame integration of a GFP gene using microhomology-mediated knock-in technology in Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
14.	Takahisa Yamashita, Taro Mito, Yoshiyasu Ishimaru, Takahito Watanabe, Sayuri Tomonari, Kohei Kawamoto and Mayuko Matuda : Generation of an enhancer-trap strain of the scalloped gene in the cricket Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
15.	Yuki Nakamura, Sayuri Tomonari, Kohei Kawamoto, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji and Taro Mito : Resolving the correlation between phenotype and genotype in a segmentation gene even-skipped in the cricket Gryllus bimaculatus, 52nd Annual Meeting of the Japanese Society of Developmental Biologists, May 2019.
16.	Yu-ki Nakamura, Ko-hei Kawamoto, Sayuri Tomonari, Takahito Watanabe, Yoshiyasu Ishimaru, Taro Mito and Sumihare Noji : Gene knock-out analysis of a segmentation gene even-skipped in the cricket Gryllus bimaculatus, Joint Annual Meeting of JSDB 51st and JSCB 70th, Jun. 2018.
17.	上村菜月, 友成さゆり, 渡辺崇人, 松岡佑児, 石丸善康, 野地澄晴, 三戸太郎 : 遺伝子ノックイン技術の応用によるレポーターコオロギ系統の作製, 第88回日本動物学会, 2017年9月.
18.	Matsuda Mayuko, Matsuoka Yuji, Yoshiyasu Ishimaru, Sayuri Tomonari, Takahito Watanabe, Sumihare Noji and Taro Mito : Functional analysis of a Hox gene, abdominal-A, in the cricket Gryllus bimaculatus using a CRISPR/Cas9-mediated gene knock-in system, Japanese Society of Developmental Biologists, May 2017.
19.	Nakamura Yu-Ki, Kawamoto Kohei, Sayuri Tomonari, Matsuda Mayuko, Takahito Watanabe, Yoshiyasu Ishimaru, Uemura Natsuki, Sumihare Noji and Taro Mito : even-skipped is required for segmentation and elongation of embryos in the cricket Gryllus bimaculatusas revealed by CRISPR/Cas9-based gene knock-out., Japanese Society of Developmental Biologists, May 2017.
20.	川本晃平, 友成さゆり, Yuji Matsuoka, 渡辺崇人, 石丸善康, 野地澄晴, 三戸太郎 : even-skipped acts principally as a gap gene in the cricket Gryllus bimaculatus as revealed by CRISPR/Cas9-based gene knockout analysis, JSDB Special Symposium: Frontier of Developmental Biology Hosted by JSDB, 2016年6月.
21.	友成さゆり, 川本晃平, 松岡佑児, 渡辺崇人, 石丸善康, 野地澄晴, 三戸太郎 : CRISPR/Cas9システムを用いた遺伝子ノックアウトによるコオロギ胚発生制御メカニズムの解析, 第38回日本分子生物学会年会, 2015年12月.
22.	石丸善康 : 脚再生の分子メカニズムーモデル昆虫コオロギについての研究ー, 再生生物学シンポジウム, 2015年8月.
23.	Akihiro Yasue, Hitomi Kono, Tetsuya Bando, Yoshiyasu Ishimaru, Junji Inoue, Takahiro Watanabe, Seiichi Oyadomari, Sumihare Noji, Taro Mito, Hideyo Ohuchi and Eiji Tanaka : Study of Pax6-deficient mosaic mice generated by the CRISPR/Cas system, 第48回日本発生生物学会, Jun. 2015.
24.	河野仁美, 泰江章博, 石丸善康, 井上順治, 渡辺崇仁, 板東哲哉, 親泊政一, 野地澄晴, 三戸太郎, 山本卓, 田中栄二, 大内淑代 : CRISPR/CasシステムによるPax6 遺伝子破壊マウスの解析, 第37回日本分子生物学会, 2014年11月.
25.	板東哲哉, Yoshiyasu Ishimaru, 木田琢郎, 三戸太郎, 大内淑代 and 野地澄晴 : Molecular mechanism of regulation of blastemal cell proliferation during leg regeneration in Gryllus bimaculatus, 第35回日本分子生物学会年会, Dec. 2012.
26.	石丸善康, 吉岡秀文, 笠原恵, 福井由宇子, 諸橋憲一郎 : ニワトリ性決定遺伝子の検索, 第30回日本分子生物学会年会・第80回日本生化学会大会, 2007年12月.
27.	Yoshiyasu Ishimaru, Tomoko Komatsu, Yuko Katoh-Fukui, Megumi Kasahara, Yoshiro Toyama, Mamiko Maekawa, Kiyotaka Toshimori, Hidefumi Yoshioka and Ken-ichirou Morohashi : Mechanism of asymmetric ovarian development in the chicken, 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006.
28.	石丸善康, 小松朋子, 笠原恵, 前川眞見子, 福井由宇子, 諸橋憲一郎, 吉岡秀文 : ニワトリ卵巣の左右非対称形成におけるPITX2及びレチノイン酸シグナルの関与, 第38回日本発生生物学会, 2005年6月.
29.	石丸善康, 吉岡秀文, 諸橋憲一郎 : 鳥類卵巣の左右非対称形成におけるレチノイン酸シグナルの関与, 第26回日本分子生物学会年会, 2003年12月.
30.	吉岡秀文, 石丸善康, 杉山紀之, 笠原恵, 諸橋憲一郎 : Mesonephric Fgf9 is the Initiation Signal for Gonadal Formation in Chick, 第76回日本生化学会大会, 2003年10月.
31.	石丸善康, 杉山紀之, 笠原恵, 諸橋憲一郎, 吉岡秀文 : 生殖腺形成におけるレチノイン酸シグナルの関与, 第36回日本発生生物学会, 2003年6月.
32.	吉岡秀文, 石丸善康, 杉山紀之, 恒川直樹, 野瀬俊明, 笠原恵, 諸橋憲一郎 : 生殖腺の形成におけるFGFシグナルの関与, 第25回日本分子生物学会年会, 2002年12月.
33.	石丸善康, 吉岡秀文, 目野主税, 椙原穂, 伊藤裕之, 大内淑代, 濱田博司, 野地澄晴 : ニワトリ胚の左右軸決定のメカニズム, 第21回日本分子生物学会年会, 1998年12月.
34.	石丸善康, 大内淑代, 吉岡秀文, 野地澄晴 : ニワトリ胚における前肢，後肢特異化のメカニズムの解明, 第31回日本発生生物学会, 1998年5月.
35.	吉岡秀文, 目野主税, 小柴和子, 西条幸男, 椙原穂, 石丸善康, 伊藤裕之, 大内淑代, 濱田博司, 野地澄晴 : マウス，ニワトリ初期胚におけるRieg遺伝子の左右非対称性発現, 第31回日本発生生物学会, 1998年5月.
36.	吉岡秀文, 竹内純, 大内淑代, 小柴和子, 石丸善康, 小椋利彦, 小椋恵子, 高橋直樹, 野地澄晴 : ニワトリ胚のFGFで誘導された過剰肢Dasokuは前肢ー後肢のキメラである, 第20回日本分子生物学会年会, 1997年12月.
37.	石丸善康, 竹内純, 松本健, 高橋将文, 吉岡秀文, 大内淑代, 小椋利彦, 小椋恵子, 高橋直樹, 野地澄晴 : ニワトリ胚側腹部のDasokuは翼か脚か, 第30回日本発生生物学会, 1997年5月.
38.	吉岡秀文, 大内淑代, 石丸善康, 西郷薫, 石川哲也, 濃野勉, 野地澄晴 : Drosophila DFR1(受容体型チロシンキナーゼ)はFGFレセプターである, 第29回日本発生生物学会, 1996年5月.
39.	吉岡秀文, 大内淑代, 石丸善康, 西郷薫, 石川哲也, 濃野勉, 野地澄晴 : ショウジョウバエFGF受容体ホモログ(DFR)はXenopus 受精卵で機能する, 第18回日本分子生物学会年会, 1995年12月.
40.	吉岡秀文, 大内淑代, 石丸善康, 丹羽尚, 川上泰彦, 石川哲也, 濃野勉, 野地澄晴 : 胚発生における Sonic hedgehog の機能, 第68回日本生化学, 1995年9月.
41.	吉岡秀文, 大内淑代, 田代康介, 塩川光一郎, 石丸善康, 西郷薫, 川上泰彦, 濃野勉, 野地澄晴 : 優性欠損型 FGF レセプターによる Xenopus 胚の sonic hedgehog の発現変化, 第28回日本発生生物学会, 1995年5月.

特許:

1.	野地澄晴, 石丸善康 : 害虫駆除成分のスクリーニング方法, 特願2015-207010 (2015年10月), 特開2017-77212 (2017年4月), .

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

細胞老化誘導に関わる酸化ストレス発生の分子機構の解明と関連microRNAの同定 (研究課題/領域番号: 22K05678 )
マクロファージの機能が展開する脱分化の分子基盤解明と関連microRNAの同定 (研究課題/領域番号: 19K06691 )
不完全変態昆虫モデルシステムを用いた後胚期形態形成の制御機構解明 (研究課題/領域番号: 17H03945 )
昆虫脚再生における幹細胞群形成の時空間的制御機構の解明 (研究課題/領域番号: 16K15068 )
TALEN法により疾患ゲノムを修復したiPS細胞を用いた自家臓器再生技術開発 (研究課題/領域番号: 25640106 )
研究者番号（50435525）による検索

講師 : 石丸善康

研究活動

個人のホームページ

専門分野

研究テーマ

著書・論文

著書:

学術論文(審査論文):

総説・解説:

国際会議:

国内講演発表:

特許:

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

講師 : 石丸 善康

研究活動

個人のホームページ

専門分野

研究テーマ

著書・論文

著書:

学術論文(審査論文):

総説・解説:

国際会議:

国内講演発表:

特許:

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

講師 : 石丸善康