Effect of anti- Dickkopf1 (DKK-1) antibodies on osteogenic differentiation of bone marrow cells and osteoporosis (anti DKK-1 antibody, osteogenic differentiation, osteoporosis)
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Effect of bone formation using TNF-α treated carbonate apatite and hydroxyapatite (carbonate apatite, tumor necrosis factor-α, bone formation)
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Evaluation of masticator and satisfaction of making new full denture patient (full denture, masticator, satisfaction)
Book / Paper
Academic Paper (Judged Full Paper):
1.
Maki Hosoki, Mayu Miyagi, Kazuo Okura, Miho Inoue, Masamitsu Ohshima, Yoshitaka Suzuki, Aya Ozawa, Akari Shibagaki, Taniwaki Tatsuya, Kouhei Kamoi, Noriyuki Bando, Yasutomo Yoshihara, Mizuki Shinkai, Toyoko Tajima, Jaime Moreno Fabillar and Yoshizo Matsuka : Proposals and evaluations for the enhancement of CAD/CAM education, Journal of Prosthodontic Research, 69, 2, 133-135, 2025.
Toyoko Tajima, Maki Hosoki, Mayu Miyagi, Miho Inoue, Aya Ozawa, Mizuki Shinkai, Mio Naritani, Yoshiaki Kubo, Raman Lakshmi Swarna, Parimal Chavan, Kazuyuki Koike and Yoshizo Matsuka : Correlation between pierced earrings and metal allergy prevalence in Tokushima University hospital: A 15-year retrospective analysis, Scientific Reports, 15, 1, 10939, 2025.
(Summary)
In Japan, metal allergies are becoming increasingly prevalent, raising concerns for public health. This study examined metal allergy characteristics, patient histories, and clinical signs associated with patch test results over a 15-year period at the Dental Metal Allergy Clinic of Tokushima University Hospital. A retrospective analysis of 1085 patients revealed that 65.4% tested positive for at least one metal allergen, with palladium chloride, nickel sulfate, potassium dichromate, and cobalt chloride identified as the most common allergens. Female patients were disproportionately affected, accounting for 78.4% of the study population. Notably, there was a substantial increase in patients reporting inflammation due to pierced earrings, increasing from 5.0% in 2005 to 43.2% in 2020, particularly among females. Patients with a history of inflammation from earrings had an 81.3% prevalence of metal allergies, which was significantly higher than the 60.4% reported in those without such a history (chi-square test, p < 0.001). These findings suggest a strong link between earrings and metal allergies, underscoring the need for improved education, early detection, and preventive strategies to address the growing impact of metal allergies on public health.
Yoshitaka Suzuki, Kazuo Okura, Toyoko Tajima, Junhel Dalanon, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Mayu Miyagi, Daisuke Ikutame and Yoshizo Matsuka : Essence of diagnosis and management of sleep bruxism, Journal of Oral Health and Biosciences, 36, 2, 22-29, 2024.
Huijiao Yan, Masamitsu Ohshima, Raju Raju, S Raman, Kazumitsu Sekine, Arief Waskitho, Miho Inoue, Masahisa Inoue, Otto Baba, Tsuyoshi Morita, Mayu Miyagi and Yoshizo Matsuka : Dentin-pulp complex tissue regeneration via three-dimensional cell sheet layering, Tissue Engineering. Part C, Methods, 27, 10, 559-570, 2021.
(Summary)
The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscope (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. After this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared with that in the single cell sheet group ( < 0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure. Impact statement The dentin-pulp complex can be destroyed by deep caries and trauma, which may cause pulpitis and progress to irreversible pulpitis, apical periodontitis, and even tooth loss. Current treatments cannot maintain pulp health, and teeth can become brittle. We developed a three-dimensional (3D) layered cell sheet using dental pulp cells and odontogenic differentiation of pulp cells for dentin-pulp complex regeneration. Our layered cell sheet enables the regeneration of an organized 3D dentin-pulp-like structure comparable with that of natural teeth. This layered cell sheet technology may contribute to dentin-pulp complex regeneration and provide a novel method for complex tissue engineering.
Rika Hayama, Kazuo Okura, Masamitsu Ohshima, Maki Hosoki, Yoshitaka Suzuki, Mayu Miyagi, Miho Inoue, Takuma Iwasa, Junhel Dalanon, Raju Resmi, Omar Maningo Rodis and Yoshizo Matsuka : Longitudinal comparison between flipped classroom and team-based learning in a prosthodontic class, Journal of Oral Health and Biosciences, 34, 1, 1-10, 2021.
(Summary)
Active learning is a concept that allows students to study and learn actively by themselves to get knowledge. There are several methods of active learning, including flipped classroom (FC) and team-based learning (TBL). In FC, students are required to study before classes. In TBL, students also study before class, take individual readiness assurance test (iRAT) and team readiness assurance test (tRAT), then discuss group assignment projects (GAPs) during class. The purpose of this study was to compare the effectiveness between FC and TBL.<br> The effectiveness of FC and TBL was assessed from the results of the term-end examinations, questionnaires and practice examinations. To check the difficulty of the term-end examinations, control dentists took the same examinations and we calculated the equating score with item response theory.<br> Statistical analysis showed that the correct answer rate in term-end examinations was significantly different in comparison with the time of the trial, and for the participants (students and dentists). The term-end examination score of FC and TBL did not show a statistical difference. The student questionnaire showed that TBL had higher scores than FC on various factors such as student positive attitude, preparation, ingenuity of teacher and achieving the class goals. The crown & bridge score from the practice examination of 6<sup>th</sup>-year students who had FC + TBL were constantly higher than the Japanese national average score. The identification index of FC and TBL did not show the statistical difference and there was no statistical difference on item response theory between FC and TBL.
Hiroo Kawahara, Miho Inoue, Kazuo Okura, Masamitsu Ohshima and Yoshizo Matsuka : Risk factors for tooth loss in patients with 25 remaining teeth undergoing mid-long-term, International Journal of Environmental Research and Public Health, 18, 13, 7174, 2021.
Yoshizo Matsuka, Masamitsu Ohshima, Kazuo Okura, Maki Hosoki, Yoshitaka Suzuki, Mayu Miyagi and Miho Inoue : インドネシアの歯学部における学部教育, The Journal of Japan Association of Oral Rehabilitation, 33, 1, 53-57, 2020.
(Keyword)
Undergraduate student education / dental school / Indonesia
H Kawahara, Miho Inoue, Kazuo Okura, Masamitsu Ohshima and Yoshizo Matsuka : Risk factors for tooth loss in patients undergoing mid long-term maintenance: A retrospective study, International Journal of Environmental Research and Public Health, 17, 17, E6258, 2020.
(Summary)
In this retrospective study, we identified risk factors for tooth loss in patients undergoing mid-long-term maintenance therapy. We surveyed 674 maintenance patients for ≥5 years after active treatment who visited a dental clinic between January 2015 and December 2016. Of these, 265 were men (mean age 54.6 ± 8.0 years old) and 409 were women (mean age 54.0 ± 7.9 years old). Study variables included patient compliance, sex, number of teeth lost, cause of tooth loss (dental caries, periodontal disease, root fracture, others, vital or non-vital teeth), age at start of maintenance, number of remaining teeth at start of maintenance, smoking, use of salivary secretion inhibitors, presence of diabetes mellitus, condition of periodontal bone loss, and use of a removable denture. Most lost teeth were non-vital teeth (91.7% of all cases) and the most common cause of tooth loss was tooth fracture (62.1% of all cases). A statistically significant risk factors for tooth loss was number of remaining teeth at the start of maintenance ( = 0.003).
Resmi Raju, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Arief Waskitho, Otto Baba, Masahisa Inoue and Yoshizo Matsuka : Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet, Scientific Reports, 10, 1, 1656, 2020.
(Summary)
Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
Takuma Iwasa, S Afroz, Miho Inoue, Rieko Arakaki, Masamitsu Ohshima, R Raju, Arief Waskitho, Inoue Masahisa, Otto Baba and Yoshizo Matsuka : IL-10 and CXCL2 in trigeminal ganglia in neuropathic pain, Neuroscience Letters, 703, 132-138, 2019.
Mio Naritani, Miho Inoue, Resmi Raju, Mayu Miyagi, Masamitsu Ohshima and Yoshizo Matsuka : Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α), Journal of Hard Tissue Biology, 28, 2, 99-108, 2019.
S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : CGRP induces differential regulation of cytokines from satellite glial cells in trigeminal ganglia and orofacial nociception, International Journal of Molecular Sciences, 20, 3, 711, 2019.
(Summary)
Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6-24 h (paired- test, < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett's post hoc test, < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (-test, < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception.
Miho Inoue, Mio Naritani, R Raju, Mayu Miyagi, Masamitsu Ohshima, Inoue Masahisa and Yoshizo Matsuka : Effect of short-term Tumour Necrosis Factor-alpha (TNF-α) -stimulation on the growth and differentiation of MC3T3-E1 osteoblast-like cells, Journal of Hard Tissue Biology, 27, 3, 213-218, 2018.
Keisuke Nishigawa, Rika Hayama, Ktsushi Omoto, Kazuo Okura, Toyoko Tajima, Yoshitaka Suzuki, Maki Hosoki, Mayu Miyagi, Miho Inoue, Omar Maningo Rodis and Yoshizo Matsuka : Validity of peer evaluation for team-based learning in a dental school in Japan, Journal of Dental Education, 81, 12, 1451-1456, 2017.
(Summary)
The aim of this study was to determine the validity of peer evaluation for team-based learning (TBL) classes in dental education in comparison with the term-end examination records and TBL class scores. Examination and TBL class records of 256 third- and fourth-year dental students in six fixed prosthodontics courses from 2013 to 2015 in one dental school in Japan were investigated. Results of the term-end examination during those courses, individual readiness assurance test (IRAT), group readiness assurance test (GRAT), group assignment projects (GAP), and peer evaluation of group members in TBL classes were collected. Significant positive correlations were found between all combinations of peer evaluation, IRAT, and term-end examination. Individual scores also showed a positive correlation with group score (total of GRAT and GAP). From the investigation of the correlations in the six courses, significant positive correlations between peer evaluation and individual score were found in four of the six courses. In this study, peer evaluation seemed to be a valid index for learning performance in TBL classes. To verify the effectiveness of peer evaluation, all students have to realize the significance of scoring the team member's performance. Clear criteria and detailed instruction for appropriate evaluation are also required.
(Keyword)
Education, Dental / Educational Measurement / Group Processes / Humans / Japan / Learning / Peer Group / Reproducibility of Results / Schools, Dental / Teaching
Miho Inoue, KAWAHARA Hiroo, Kazuo Okura, Mayu Miyagi, NARITANI Mio, Takuma Iwasa, OMOTO Katsuhiro, Omar Maningo Rodis and Yoshizo Matsuka : Frequency of congenitally missing permanent teeth at a primary dental care clinic in Tokushima, Japan, The Journal of Japan Association of Oral Rehabilitation, 30, 1, 67-70, 2017.
(Keyword)
Congenitally missing permanent teeth / hypodontia / primary dental care
Dexmedetomidine, a highly selective agonist of α2-adrenoceptors, is a commonly used sedative; however, a potent anti-inflammatory effect has also been found. In the present study we evaluated the inhibitory effect of locally injected dexmedetomidine on inflammatory responses in the injected region. Local inflammation was induced in the hindpaws of male mice (aged 6-8 weeks) by intraplantar injection of lambda-carrageenin. To offset the central effect of tested agents, different agents were blindly injected into the left and right paws in the pairs of comparison. The effect of dexmedetomidine on edema (increase in paw volume), the accumulation of leukocytes, and production of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were evaluated after carrageenin injection, using water displacement plethysmometry, histological imaging, immunohistochemistry, and Western blotting analysis. Furthermore, we also evaluated the effect of yohimbine, a full antagonist of α2-adrenoceptors, and phenylephrine, an agonist of the α1-adrenoceptor, on dexmedetomidine's action on inflammatory responses. Paw volume and amount of leukocytes in the injected region significantly increased after the injection of carrageenin. Similarly, TNF-α and COX-2 production was found in the subcutaneous region injected with carrageenin, 4 hours after injection. Dexmedetomidine significantly inhibited all increases in paw volume, leukocytes, and production of TNF-α and COX-2. Furthermore, yohimbine significantly antagonized the anti-inflammatory effects of dexmedetomidine, whereas phenylephrine did not significantly alter them. The findings suggest that locally injected dexmedetomidine exhibits an anti-inflammatory effect against local acute inflammatory responses, mediated by α2-adrenoceptors.
P. Andrea Rodriguez, Miho Inoue, Toshiyuki Tanaka, Michihiro Miyake, M. Ana Sfer, Etsuo Kishimoto, Hidetsugu Tsujigiwa, S. Rosario Rivera and Hitoshi Nagatsuka : Effect of CaTiO3-CaCO3 prepared by alkoxide method on cell response, Journal of Biomedical Materials Research. Part A, 93, 1, 297-303, 2010.
(Summary)
In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental composition of CaTiO(3)-CaCO(3) prepared by alkoxide method, CaTiO(3)-aC elaborated by modified thermal decomposition method, commercially-prepared CaTiO(3), and the effect of these materials on the bone marrow stromal cell. Hydroxyapatite was used as positive control material. We examined the cellular proliferation, osteoblastic differentiation, and mineralization of KUSA/A1 cells cultured with the materials. The results showed that CaTiO(3)-CaCO(3) and CaTiO(3)-aC contained evidence of calcium carbonate enhancing cell proliferation, osteoblastic differentiation, and mineralization. On the contrary, the commercially-prepared CaTiO(3) revealed absence of calcium carbonate with lower cell response than the other groups. The results indicated that calcium carbonate could play a key role in the cell response of CaTiO(3) material. In conclusion, our findings suggest that CaTiO(3)-CaCO(3) could be considered an important candidate as a biomaterial for medical and dental applications.
Miho Inoue, P. Andrea Rodriguez, Noriyuki Nagai, Hitoshi Nagatsuka, Z. Racquel Legeros, Hidetsugu Tsujigiwa, Masahisa Inoue, Etsuo Kishimoto and Shin Takagi : Effect of fluoride-substituted apatite on in vivo bone formation, Journal of Biomaterials Applications, 25, 8, 811-824, 2010.
(Summary)
Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations (l-FAp: 0.48 wt%, m-FAp: 0.91 wt%, h-FAp: 2.23 wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application.
Miho Inoue, AP Rodriguez, T Takagi, N Katase, M Kubota, N Nagai, H Nagatsuka, M Inoue, N Nagaoka, S Takagi and K Suzuki : Effect of a new titanium coating material (CaTiO3-aC) prepared by thermal decomposition method on osteoblastic cell response., Journal of Biomaterials Applications, 24, 7, 657-672, 2010.
(Summary)
Titanium and hydroxyapatite (HA) are widely used as biomaterials for dental and medical applications. HA-coated titanium implants have excellent biocompatibility and mechanical properties. However, the adherence of HA film formed on titanium substrate is weak because of the lack of chemical interaction between HA and titanium. A solution to this problem is to form an intermediate film on titanium substrate, which provide excellent adherence to both titanium substrate and HA. We developed a novel biomaterial called calcium titanate-amorphous carbon (CaTiO(3)-aC) coating prepared by modified thermal decomposition method. The purpose of this study was to evaluate the effect of CaTiO(3)-aC and HA coating (positive control), and Ti (negative control) on osteoblastic (MT3T3-E1) cell responses. An increased cellular proliferation was observed in CaTiO(3)-aC coating compared to HA coating. The maximum expressions of ALP activity, Col I and ALP mRNA were higher and achieved in shorter period of time in CaTiO(3)-aC coating compared to others. These results demonstrated that CaTiO(3)-aC promoted better cell attachment, cellular proliferation, and osteoblastic differentiation compared with HA. In conclusion, we suggested that CaTiO(3)-aC could be considered as an important candidate as a coating material.
AP Rodriguez, H Tsujigiwa, M Gunduz, B Cengiz, N Nagai, R Tamamura, SS Borkosky, T Takagi, Miho Inoue and H Nagatsuka : Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells., Biocell, 33, 1, 39-47, 2009.
(Summary)
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
(Keyword)
Animals / Bone Regeneration / Bone and Bones / Cell Differentiation / Cell Line / Collagen Type I / Dental Papilla / Diffusion Chambers, Culture / Gene Expression / Male / Mice / Mice, SCID / Odontoblasts / Odontogenesis / Osteoblasts / Osteocalcin / Osteonectin / Osteopontin / Protein Biosynthesis / Sialoglycoproteins
(Link to Search Site for Scientific Articles)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19499885
GS Sathi, H Nagatsuka, R Tamamura, M Fujii, M Gunduz, Miho Inoue, RS Rivera and N Nagai : Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma., Histopathology, 53, 4, 458-467, 2008.
(Summary)
To study the stromal variation and role of stromal-tumour cell interaction in impaired bone formation as well as enhanced bone resorption in ameloblastoma. Four types of stroma were observed histologically; fibrous, desmoplastic, myxoid and myxoid with hyalinization. Osteoblast and osteoclast were counted using haematoxylin and eosin sections and immunohistochemistry with CD68. After histomorphometric analysis, only fibrous and myxoid types of stroma were distinctly identified. Secreted frizzled-related peptide (sFRP)-2, transforming growth factor-beta 1 and receptor activator of nuclear factor-kappaB ligand (RANKL) revealed strong expression in myxoid type compared with the normal stroma. Bone morphogenetic protein (BMP)-2 was negative in myxoid type, but positive in normal stroma. Fibrous-type stroma showed weak expression of all antigens except RANKL compared with myxoid type. The results suggest that stroma does not act only in bone resorption, but also in the suppression of new bone formation. sFRP-2 is the main factor for impaired bone formation. The expression of markers related to osteoclastogenesis and suppression of osteoblast formation is higher in myxoid-type than in fibrous-type stroma. Tumour cells create a favourable environment for impaired bone formation by secreting sFRP-2 as well as bone resorption by secreting RANKL and interleukin-6.
(Keyword)
Adult / Ameloblastoma / Antigens, CD / Antigens, Differentiation, Myelomonocytic / Bone Neoplasms / Bone Resorption / Bone and Bones / Female / Humans / Interleukin-6 / Jaw Neoplasms / Male / Membrane Proteins / Middle Aged / Neoplasm Invasiveness / Osteoblasts / Osteoclasts / Osteogenesis / RANK Ligand / Stromal Cells / Tumor Cells, Cultured
C Kuroda, S Kubota, K Kawata, E Aoyama, K Sumiyoshi, M Oka, Miho Inoue, S Minagi and M Takigawa : Distribution, gene expression, and functional role of EphA4 during ossification., Biochemical and Biophysical Research Communications, 374, 1, 22-27, 2008.
(Summary)
EphA4 receptor tyrosine kinase has been shown to be critically involved in neural tissue development. Here, we found EphA4 was also distributed among hypertrophic chondrocytes and osteoblasts in the growth plate of developing mouse long bones. In vitro evaluation revealed that ephA4 expression was elevated upon hypertrophic differentiation of chondrocytes and that markedly stronger expression was observed in osteoblastic SaOS-2 than chondrocytic HCS-2/8 cells. Of note, RNAi-mediated silencing of ephA4 in SaOS-2 cells resulted in the repression of osteocalcin gene expression and alkaline phosphatase activity. Interestingly, confocal laser-scanning microscopic analysis revealed the presence of EphA4 molecules in the nucleus as well as on the surface of SaOS-2 cells. These findings are the first indication of a critical role of EphA4 in ossification, especially at the final stage in which osteoblasts and hypertrophic chondrocytes play major roles.
M Okauchi-Yabuuchi, R Tamamura, N Nagaoka, S Takagi, E Kishimoto, T Takagi, AP Rodriguez, Miho Inoue, H Nagatsuka, M Akao and N Nagai : Chemical Analysis of a Novel Coating Material, CaTiO3-aC., Journal of Hard Tissue Biology, 17, 3, 115-120, 2008.
(Summary)
Abstract:In the current study, we compared the recently developed CaTiO3-amorphous carbon (CaTiO3-aC) as a bone inducing coating material with HA from aspects of surface electric charge and solubility. CaTiO3-aC had negative surface electric charge similar to HA. Thus similar tissue reaction and bone inducing ability were considered to obtain. On the other hand solubility of CaTiO3-aC coating was lower than HA. Moreover though CaTiO3-aC itself showed low solubility, CaCO3 was found to be included in it, and long-term slow Ca2+ release occurred. Thus the sample was suggested to be used as an ion exchange material. When the implant using Ti/CaTiO3-aC/HA double layer coating was developed, first bioactive state of the implant will continue due to HA character. Then even early resorption of HA occurs, bioactive state will continue due to CaTiO3-aC layer. Therefore Ti base will not expose. This result is supposed to contribute long term success of the implant.
(Keyword)
CaTiO3-aC / Hydroxyapatite / Implant / Coating material / Titanium
N Nagai, M Okauchi, AP Rodriguez, M Gunduz, H Hu, M Kubota, N Nagaoka, Miho Inoue, H Nagatsuka, T Takagi and M Akao : Development of New Titanium Coating Material (CaTiO3-aC) with Modified Thermal Decomposition Method., Journal of Hard Tissue Biology, 17, 2, 47-54, 2008.
(Summary)
We developed a novel method for titanium coating material using modified thermal decomposition technique, specifically focusing on presence of CaTiO3 and carbon C. Two layers of thin film coating composed of CaTiO3-amorphous carbon compound (CaTiO3-aC) and hydroxyapatite (HA) were generated on titanium surface. In this method, ratios of Ca/P as well as Ca/Ti, sintering temperature and sintering velocity were carefully planned. Within crystal structure of CaTiO3 granules, 4 atomic % of carbon in amorphous state was included. By our method, inclusion of carbon in HA suggested formation of carbonate apatite. Regarding with attachment-detachment experiment at titanium surface, adhesion strength was 2.5 times stronger in CaTiO3-aC/HA coating substrate as compared to HA only coating material. Results of the novel developed modified thermal decomposition method suggested that the 2 layers biomaterial composed of CaTiO3-aC (0.6 μm) and carbonate apatite-included HA (2.4 μm) can be used as a coating material on titanium surface.
(Keyword)
Biomaterial / Titanium / Carbon / HA / Coating material / CaTiO3-aC
M Hoshijima, T Hattori, Miho Inoue, D Araki, H Hanagata, A Miyauchi and M Takigawa : CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1., FEBS Letters, 580, 5, 1376-1382, 2006.
(Summary)
Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion.
RG Craig, AR Kamer, SP Kallur, Miho Inoue and DP Tarnow : Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig., The Journal of Oral Implantology, 32, 5, 228-236, 2006.
(Summary)
We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.
H Tsujigiwa, H Nagatsuka, M Gunduz, AP Rodriguez, RS Rivera, RZ LeGeros, Miho Inoue and N Nagai : Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells., Journal of Biomedical Materials Research. Part A, 75A, 1, 210-215, 2005.
(Summary)
The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.
(Keyword)
Alkaline Phosphatase / Animals / Biocompatible Materials / Blotting, Western / Bone Marrow Cells / Bone Morphogenetic Protein 2 / Bone Morphogenetic Proteins / Bone and Bones / Cell Line / Collagen / Collagen Type I / Diffusion / Dose-Response Relationship, Drug / Electrophoresis, Polyacrylamide Gel / Humans / Mice / Recombinant Proteins / Succinates / Time Factors / Transforming Growth Factor beta
Miho Inoue, H Nagatsuka, H Tsujigiwa, M Inoue, RZ LeGeros, T Yamamoto and N Nagai : In vivo effect of fluoride-substituted apatite on rat bone., Dental Materials Journal, 24, 3, 398-402, 2005.
(Summary)
Different types of calcium phosphate compounds are commercially available for medical and dental applications as bone substitute materials. Biological apatites contain several kinds of minor elements such as carbonate (CO3), magnesium (Mg), and fluoride (F) in enamel, dentin, and bone. It has been shown that F ion and F-substituted apatite promoted osteoblast proliferation and inhibited osteoclast cell activity. The purpose of this study was to investigate the in vivo rat tibia activity on F-substituted apatite (FAp). Apatites of unsintered calcium deficient apatite (CDA), and FAps, with low, medium, and high F concentrations, were implanted in rat tibia for 1 and 2 weeks. Implanted tissues were embedded in paraffin blocks, stained with hematoxylin-eosin and histomorphometrically observed. Results showed that low F concentration induced better and faster new bone formation in vivo compared to CDA. Therefore the results suggested that F as a minor element in bone rendered a suitable effect on bone formation in vivo.
(Keyword)
Animals / Apatites / Bone Marrow / Bone Substitutes / Calcium / Coloring Agents / Fluorides / Male / Osteoblasts / Osteoclasts / Osteogenesis / Rats / Rats, Wistar / Tibia / Time Factors
H Nakamura, N Nagaoka, A Hirata, Miho Inoue, H Ozawa and T Yamamoto : Distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) in osteoclasts after calcitonin administration., Archives of Histology and Cytology, 68, 2, 143-150, 2005.
(Summary)
Scanning electron microscopy (SEM) was employed to study the effect of calcitonin on the distribution of actin filaments in osteoclasts obtained from rat tibiae. Fluorescent microscopy was also applied to examine calcitonin-induced changes in the distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) to clarify the role of ERK in the cytoskeleton of osteoclasts. SEM of control osteoclasts revealed a ring-like structure in the peripheral region. Labeled actin filaments and non-muscle myosin were detected in the peripheral region and exhibited a ring-like pattern. Immunoreactivity indicating M-Ras and ERK was also detected in the vicinity of the actin ring. After calcitonin treatment, many osteoclasts exhibited a retracted appearance and lacked a discernible actin ring. Numerous retraction fibers were found at the edge of calcitonin-treated osteoclasts. Actin filaments and non-muscle myosin were concentrated in the cytoplasm of calcitonin-treated osteoclasts, and exhibited a filamentous pattern. Labeled M-Ras and ERK also accumulated in the central region of these osteoclasts. These findings suggest that actin-myosin interaction plays an essential role in the retraction of osteoclasts induced by calcitonin. ERK may play a role in this interaction by activating myosin light chain kinase, as previously observed in smooth muscle cells.
T Yamamoto, N Nagaoka, A Hirata, H Nakamura, Miho Inoue, M Kawai and M Ikegame : Ultrastructural and imminohistochemical studies of medullary bone calcification, with special reference to sulphated glycosaminoglycans., Journal of Electron Microscopy, 54, 1, 29-34, 2005.
(Summary)
Histochemical, immunohistochemical and electron energy-loss spectroscopic studies were performed to examine the relationship between sulphated glycosaminoglycans and medullary bone calcification using oestrogen-injected male Japanese quail. Sulphated glycosaminoglycans, detected by high iron diamine (HID) or HID-thiocarbohydrazide-silver protein (HID-TCH-SP) methods, were distributed throughout the matrix of medullary bone, some periphery and extending tips of the trabeculae stained weakly, and the globular structures at osteoid areas were exclusively positive for HID-TCH-SP stain. Immunohistochemistry identified keratan sulphate located in the globular structures at osteoid areas and calcified matrix, but chondroitin-4 sulphate and chondroitin-6 sulphate were not detected in the matrix. Using electron spectroscopic imaging, sulphur was determined to be localized in the globular structures. These results demonstrate that medullary bone matrix accumulates keratan sulphate in the globular structures, which are the foci for calcification, and eventually in the calcified areas. This suggests that keratan sulphate containing sulphur is maintained in the calcified matrix. These results indicate a unique process of calcification exists in medullary bone.
(Keyword)
Animals / Bone Matrix / Bone and Bones / Calcification, Physiologic / Chondroitin Sulfates / Coturnix / Estrogens / Glycosaminoglycans / Histocytochemistry / Hydrazines / Immunohistochemistry / Keratan Sulfate / Male / Silver Proteins / Spectroscopy, Electron Energy-Loss
H Nakamura, R Kato, A Hirata, Miho Inoue and T Yamamoto : Localization of CD44 (Hyaluronan Receptor) and Hyaluronan in Rat Mandibular Condyle., The Journal of Histochemistry and Cytochemistry, 53, 1, 113-120, 2005.
(Summary)
CD44 is a multifunctional adhesion molecule that binds to hyaluronan (HA), type I collagen, and fibronectin. We investigated localization of CD44 and HA in mandibular condylar cartilage compared with the growth plate and the articular cartilage, to clarify the characteristics of chondrocytes. We also performed Western blotting using a lysate of mandibular condyle. In mandibular condyle, CD44-positive cells were seen in the surface region of the fibrous cell layer and in the proliferative cell layer. Western blotting revealed that the molecular weight of CD44 in condyle was 78 to 86 kD. Intense reactivity for HA was detected on the surface of the condyle and the lacunae of the hypertrophic cell layer. Moderate labeling was seen in cartilage matrix of the proliferative and maturative layer. Weak labeling was also seen in the fibrous cell layer. In growth plate and articular cartilage, HA was detected in all cell layers. However, chondrocytes of these cartilages did not exhibit reactivity for CD44. These results suggest that chondrocytes in the mandibular condylar cartilage differ in expression of CD44 from those in tibial growth plate and articular cartilage. Cell-matrix interaction between CD44 and HA may play an important role in the proliferation of chondrocytes in the mandibular condyle.
Miho Inoue, RZ LeGeros, M Inoue, R Rohanizadeh, L Guo, R Davidson, K Rui, H Nagatsuka and N Nagai : Effect of magnesium, strontium or fluoride ions on in vitro activities of odontoblast-like cells (MDPC-23)., Journal of Hard Tissue Biology, 14, 1, 5-12, 2005.
Miho Inoue, RZ LeGeros, M Inoue, H Tsujigiwa, H Nagatsuka, T Yamamoto and N Nagai : In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites., Journal of Biomedical Materials Research. Part A, 70A, 4, 585-593, 2004.
(Summary)
Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (beta-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 microg/mL) and beta-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites).
RG Craig, SP Kallur, Miho Inoue, PA Rosenberg and RZ LeGeros : Effect of enamel matrix proteins on the periodontal connective tissue-material interface after wound healing., Journal of Biomedical Materials Research. Part A, 69A, 1, 180-187, 2004.
(Summary)
The periodontal ligament has the potential to regenerate a complete periodontal connective tissue attachment, starting with the deposition of cementum, on pathologically exposed root surfaces as well as several materials including titanium oxide. However, most commonly used dental materials result in a fibrous encapsulation or a chronic inflammatory response after periodontal wound healing rather than the formation of a periodontal connective tissue attachment. Recently, an extract of porcine enamel matrix (Emdogain(R), EMD) has been reported inductive of cementum formation in both in vivo and in vitro studies. The aim of this study was to determine the effect of EMD, when applied to materials previously reported not supportive of periodontal connective tissue formation, on the periodontal connective tissue-material interface obtained with these materials in vivo. Bilateral osteotomies were performed on the mandible of a Yucatan minipig exposing the buccal root surface of four premolars. A series of four preparations were placed in each root surface that were subsequently filled with calcium hydroxide, gutta percha, mineral trioxide aggregate (MTA), or left unfilled. One side, in addition, received an application of EMD prior to surgical closure. A bioabsorbable surgical barrier membrane was placed over the osteotomy sites to exclude gingival connective tissue from the wound-healing environment. The mucoperiosteal flaps were then readapted and sutured in position. The animal was euthanized 10 weeks after the procedure, block sections obtained and prepared for light microscopy. Results demonstrated complete regeneration of alveolar bone and periodontal ligament in all four teeth from the EMD-treated side. Fibers from the periodontal ligament were observed to insert into a mineralized matrix consistent with cementum on all four root preparations. In contrast, massive root resorption without regeneration of alveolar bone was found on all teeth from the side not treated with EMD. The results of this pilot study suggest that the application of EMD to material surfaces that normally do not support periodontal connective tissue attachment formation can alter the type of periodontal connective tissue interface obtained with these materials.
Miho Inoue, RZ LeGeros, C Hoffman, K Diamond, PA Rosenberg and RG Craig : Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials., Journal of Biomedical Materials Research. Part A, 69A, 1, 172-179, 2004.
(Summary)
Cells within the periodontal ligament have the potential to regenerate a periodontal connective tissue attachment on pathologically exposed root surfaces as well as on several material surfaces including titanium. However, rather than a periodontal connective tissue attachment, a fibrous encapsulation or chronic inflammatory response has been reported at the material connective tissue interface for most dental materials. Cementum is the first tissue of the periodontal connective tissue attachment to develop and the secretion of enamel matrix related proteins on the newly mineralized dentin surface precedes and is thought to induce cementum formation. Enamel matrix-related proteins may also function in the adult because the application of an acid extract of porcine enamel protein matrix (Emdogain(R), EMD) on pathologically exposed root surfaces has been shown to result in cementum regeneration. Therefore, the objective of the present study was to determine whether the application of EMD to materials that do not normally support cementogenesis in vivo would alter the in vitro phenotype of periodontal ligament (PDL) cells including the synthesis of cementum-associated extracellular matrix proteins. Primary PDL cells were established from 21-day-old Sprague-Dawley rats, and were cultured on four materials commonly encountered in dental practice (gutta percha, calcium hydroxide, amalgam, and super EBA cement) with and without the application of EMD. After 7 or 14 days of culture, total-DNA content, collagen synthesis, alkaline phosphatase activity, and the synthesis of a 42-kDa cementum-associated extracellular matrix protein were determined. PDL cells cultured on all materials had decreased total DNA content. The application of EMD further decreased total DNA content. PDL cells cultured on gutta percha and calcium hydroxide with the application of EMD had similar levels of collagen synthesis and alkaline phosphatase activity but also expressed a 42-kDa cementum extracellular matrix-associated protein when compared to the other groups. These results suggest that EMD can alter the phenotype of PDL cells when cultured on these dental materials.
K Nawachi, Miho Inoue, S Kubota, K Takahashi, T Nishida, G Yoshimichi, T Nakanishi, M Kanyama, T Kuboki, H Yatani, T Yamaai and M Takigawa : Tyrosine Kinase-type receptor ErbB4 in chondrocytes : interaction with connective tissue growth factor and distribution in cartilage., FEBS Letters, 528, 1-3, 109-113, 2002.
(Summary)
In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.
Miho Inoue, T Inoue, A Miyagi, I Tanimoto, R Shingaki, h Ohta and K Fukui : Nucleotide Sequencing and Transcriptional Analysis of Two Tandem Genes Encoding Glucosyltransferase (Water- Soluble- Glucan Synthetase) in Streptococcus cricetus HS-6., Microbiology and Immunology, 44, 9, 755-764, 2000.
(Summary)
Two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (GTF-S) were cloned from the lambda gene library of Streptococcus cricetus HS-6 (serotype a) using anti-GTF-S antibody, and the nucleotide sequences were analyzed. The two genes (ORF1 and ORF2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and C-terminal repeated units for dextran binding, and [2] a homology search revealed that the ORF1 and ORF2 products are homologous to the GtfS protein (77.4%) of S. downei Mfe28 and GtfT protein (83.8%) of S. sobrinus OMZ176, respectively, which are both known to have GTF-S activity. Therefore, ORF1 and ORF2 might be designated gtfS and gtfT of S. cricetus, respectively. A Northern blotting and RNase protection assay suggested that the gtfS and gtfT of S. cricetus are transcribed as a single bicistronic mRNA as well as separate monocistronic mRNAs. Primer extension analysis indicated multiple transcriptional start points for each gene.
(Keyword)
Amino Acid Sequence / Base Sequence / Cloning, Molecular / Glucosyltransferases / Molecular Sequence Data / Open Reading Frames / Streptococcus / Transcription, Genetic
S Kawamata, K Shimoharai, Y Imura, Miho Inoue, Y Ichinose, T Shiraishi, H Kunoh and T Yamada : Temporal and Spatial Pattern of the Pea Phenylalanine Ammonia-Lyase Gene1 Promoter in Transgenic Tabacco., Plant & Cell Physiology, 38, 7, 792-803, 1997.
Proceeding of International Conference:
1.
S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : CGRP induced glial-cytokine expression, NFkB signaling and orofacial nociception, International Association for Dental Research, Washington, D.C., Mar. 2020.
2.
R Raju, Masamitsu Ohshima, Tsuyoshi Morita, Huijiao Yan, Miho Inoue, Otto Baba, Masahisa Inoue and Yoshizo Matsuka : A double-layered complex cell sheet can regenerate the periodontal tissue in a mice model, Dentisphere, Surabayai, Nov. 2019.
3.
S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka : Orofacial nociception due to glial expressed cytokines in trigeminal ganglia, International Association for Dental Research, Vancouver, Jun. 2019.
4.
Iwasa Takuma, Rieko Arakaki, Oshima Masamitsu, S Afroz, Miho Inoue, Goto Nami, Naozumi Ishimaru and Yoshizo Matsuka : Up-regulation of inflammatory cytokines in trigeminal ganglia after infraorbital nerve constriction, The Journal of Neuroscience, Washington DC, Nov. 2017.
5.
P. Andrea Rodríguez, A. María Sánchez, Miho Inoue, Betiana Felice, Hitoshi Nagatsuka, E. Rossana Madrid, Carmelo Felice and Hidetsugu Tsujigiwa : Effect of sintering time of CaTiO3-CaCO3 in osteoblastic response of KUSA/A1 cells, IFMBE Proceedings, 49, 159-162, Jan. 2015.
Yuki Iwawaki, Hiromichi Kawano, Yasufumi Nishikawa, Miho Inoue, Susumu Abe, Yuichi Ishida, Megumi Watanabe and Yoritoki Tomotake : 10-year survey of trend and treatment progress of new patients in division of implant dentistry at our university hospital., 第54回公益社団法人日本口腔インプラント学会学術大会, Nov. 2024.
Aya Ozawa, Yoshitaka Suzuki, Toyoko Tajima, Toshinori Okawa, Kouhei Kamoi, Kazuo Okura, 谷脇 竜弥, Miho Inoue, 吉原 靖智, Masamitsu Ohshima, Susumu Abe and Yoshizo Matsuka : 睡眠時ブラキシズム患者の適応したアプライアンスの変形を長期間観察した症例, The Journal of the Japan Prosthodontic Society, May 2023.
17.
Miho Inoue, Masamitsu Ohshima, Mayu Miyagi, Daisuke Ikutame and Yoshizo Matsuka : 口腔顔面痛はアロマテラピーで軽減する∼口腔顔面痛モデル動物での評価∼, The Journal of the Japan Prosthodontic Society, May 2023.
18.
Maki Hosoki, Daisuke Ikutame, Mayu Miyagi, Kazuo Okura, Miho Inoue, OZAWA Aya, SHIBAGAKI Akari, TANIWAKI Tatsuya, Kouhei Kamoi, 伸幸 板東, Yasutomo Yoshihara, Masamitsu Ohshima, Yoshitaka Suzuki, SHINKAI Mizuki and Yoshizo Matsuka : デジタル化に対応したクラウンブリッジ補綴学実習の検討, The Journal of the Japan Prosthodontic Society, May 2023.
19.
蓮池 真由子, Miho Inoue, RAMAN SWARNA LAKSHMI, M.Fabillar Jaime, P.Perdana Lutfi, Mayu Miyagi, Daisuke Ikutame, Masamitsu Ohshima and Yoshizo Matsuka : 口腔顔面痛モデル動物を用いた精油嗅覚刺激の疼痛抑制効果∼ベルガモット，ラベンダーの検討∼, Shikoku Dental Research, Mar. 2023.
20.
Miho Inoue, RAMAN SWARNA LAKSHMI, FABILLAR JR. JAIME MORENO, 蓮池 真由子, PERDANA LUTFI PUTRA, 井上 正久, Daisuke Ikutame, Mayu Miyagi, Masamitsu Ohshima and Yoshizo Matsuka : 精油成分による口腔顔面痛モデル動物の痛み軽減とメカニズム, 徳島大学脳科学クラスター・ミニリトリート, Feb. 2023.
Maki Hosoki, Toyoko Tajima, Mayu Miyagi, Miho Inoue, Yoshiaki Kubo and Yoshizo Matsuka : 装飾品に対するニッケル表示の標準化をめざした取り組みについて, 日本皮膚免疫アレルギー学会, Dec. 2022.
23.
Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Maki Hosoki, Daisuke Ikutame, Susumu Abe, Fumiaki Kawano and Yoshizo Matsuka : アクティブラーニングの学修効果 ―オンライン授業と通常授業，TBL授業の比較―, The Journal of Japanese Dental Education Association, Jul. 2022.
24.
Maki Hosoki, Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Daisuke Ikutame, Susumu Abe, Fumiaki Kawano and Yoshizo Matsuka : 2021年度歯科補綴学実習の評価, The Journal of Japanese Dental Education Association, Jul. 2022.
25.
Toyoko Tajima, Maki Hosoki, Miho Inoue, Aya Ozawa, Mizuki Shinkai, Mayu Miyagi, Mio Naritani, Masamitsu Ohshima, 杉尾 隆夫, 山本 伊一郎 and Yoshizo Matsuka : 徳島大学病院における歯科用金属アレルギー患者の臨床統計学的検討, The Journal of the Japan Prosthodontic Society, Jul. 2022.
26.
SHINKAI Mizuki, Maki Hosoki, Yoshitaka Suzuki, Toyoko Tajima, Miho Inoue, OZAWA Aya, Yasutomo Yoshihara, Masamitsu Ohshima, 織田 英正, 本田 常晴 and Yoshizo Matsuka : レジンアレルギーのリスクと歯科治療における対応, The Journal of the Japan Prosthodontic Society, Jul. 2022.
27.
Kazuo Okura, Rika Hayama, Masamitsu Ohshima, Maki Hosoki, Yoshitaka Suzuki, Mayu Miyagi, Miho Inoue, Daisuke Ikutame, Toyoko Tajima, Yasutomo Yoshihara, Aya OZAWA, SHINKAI Mizuki and Yoshizo Matsuka : 歯科補綴学におけるアクティブラーニングの学修効果 反転授業とTBL，通常授業の比較, The Journal of the Japan Prosthodontic Society, Jul. 2022.
28.
Kazuo Okura, Rika Hayama, Masamitsu Ohshima, Maki Hosoki, Yoshitaka Suzuki, Miho Inoue, Daisuke Ikutame, Yasutomo Yoshihara, SHINKAI Mizuki, OZAWA Aya and Yoshizo Matsuka : 歯科補綴学授業におけるアクティブラーニングの学修効果―オンライン授業と通常授業，TBL授業の比較―, 大学教育カンファレンス in 徳島, Jan. 2022.
Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Maki Hosoki, Susumu Abe, Fumiaki Kawano and Yoshizo Matsuka : 歯科補綴学授業におけるアクティブラーニングの導入とその学習効果 ―オンライン授業とTBL授業の比較―, The Journal of Japanese Dental Education Association, Nov. 2021.
31.
Maki Hosoki, Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Susumu Abe, Fumiaki Kawano and Yoshizo Matsuka : コロナ禍におけるデジタル機器およびウェブを利用した歯科補綴学実習の評価, The Journal of Japanese Dental Education Association, Nov. 2021.
32.
Maki Hosoki, Yuehui Zhang, Toyoko Tajima, Miho Inoue, 井上 正久, Masamitsu Ohshima, Mayu Miyagi and Yoshizo Matsuka : モデルマウスによるチタンアレルギーの検討, Journal of the Japanese Association for Dental Science, Sep. 2021.
33.
Yasutomo Yoshihara, Yoshitaka Suzuki, Kazuo Okura, Shuji Shigemoto, Susumu Abe, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Eiichi Bando and Yoshizo Matsuka : 顎運動を伴うRMMAの三次元顎運動解析を行った症例, The Journal of the Japan Prosthodontic Society, Jun. 2021.
34.
Yuehui Zhang, Maki Hosoki, Masamitsu Ohshima, Mayu Miyagi, 成谷 美緒, Miho Inoue and Yoshizo Matsuka : 金属アレルギー患者群と健常者群における特異的マイクロRNAの同定, The Journal of the Japan Prosthodontic Society, Jun. 2021.
35.
Masamitsu Ohshima, Raju Resmi, Yan Huijiao, Miho Inoue and Yoshizo Matsuka : 骨‐歯根膜線維の複合組織立体形成による歯周組織再生技術の開発, 第20回日本再生医療学会総会, Mar. 2021.
Maki Hosoki, Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Susumu Abe, Fumiaki Kawano and Yoshizo Matsuka : 新型コロナウイルス感染症対策を踏まえた冠橋義歯学実習の検討, The Journal of Japanese Dental Education Association, Sep. 2020.
42.
Kazuo Okura, Miho Inoue, Yoshitaka Suzuki, Maki Hosoki, Fumiaki Kawano and Yoshizo Matsuka : 歯科補綴学授業におけるアクティブラーニングの導入とその学習効果 ―通常授業，TBL，反転授業の比較―, The Journal of Japanese Dental Education Association, Sep. 2020.
43.
Masamitsu Ohshima, Raju Resmi, Miho Inoue, Mayu Miyagi and Yoshizo Matsuka : 骨‐歯根膜線維の複合組織形成による三次元組織再生技術の開発, 公益社団法人日本補綴歯科学会第129回学術大会, Jun. 2020.
44.
Takuma Iwasa, Miho Inoue, Raju Resmi, Masamitsu Ohshima and Yoshizo Matsuka : IL-10は神経障害性疼痛を抑制する, The Journal of the Japan Prosthodontic Society, Jun. 2020.
45.
Maki Hosoki, Zhang Yuehui, Masamitsu Ohshima, Miho Inoue, Mio Naritani, Mayu Miyagi and Yoshizo Matsuka : 血中マイクロRNAを介した金属アレルギーの感作・発症機序の解明, Journal of the Japanese Association for Dental Science, 39, 58, Mar. 2020.
Raju Resmi, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Otto Baba, Inoue Masahisa and Yoshizo Matsuka : Development of a multilayered complex cell sheet to regenerate periodontal tissue, Tokushima University Bioscience Retreat, Sep. 2019.
Maki Hosoki, Yuehui Zhang, Masamitsu Ohshima, Miho Inoue, 成谷 美緒, Mayu Miyagi and Yoshizo Matsuka : 血中マイクロRNAを介した金属アレルギーの感作・発症機序の解明, 歯科医学を中心とした総合的な研究を推進する集い, Aug. 2019.
54.
Raju Resmi, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Otto Baba, 井上 正久 and Yoshizo Matsuka : 骨芽細胞と歯根膜細胞との立体形成複合細胞シートによる歯周組織再生の確立, 硬組織再生生物学会, Aug. 2019.
55.
Kazuo Okura, Miho Inoue, Maki Hosoki, Fumiaki Kawano and Yoshizo Matsuka : 卒前専門基礎教育におけるアクティブラーニングの導入とその学習効果―反転授業とTBLの比較―, The Journal of Japanese Dental Education Association, Jul. 2019.
56.
Miho Inoue, Raju Resmi, Takuma Iwasa, 秋山 謙太郎, Masamitsu Ohshima, 窪木 拓男 and Yoshizo Matsuka : 抗Dickkopf1 (DKK-1) 抗体による骨分化能メカニズムの解明, The Journal of the Japan Prosthodontic Society, May 2019.
S Afroz, Iwasa Takuma, Masamitsu Ohshima, Rieko Arakaki, Miho Inoue, Naozumi Ishimaru and Yoshizo Matsuka : Cytokine released by satellite glial cells and pain transmission in sensory ganglions, Japan Association for Dental Research, Nov. 2017.
67.
Hiroh Kawahara, Miho Inoue, Kazuo Okura, Masamitsu Ohshima and Yoshizo Matsuka : 長期的メインテナンス患者における歯の喪失に関する後ろ向き調査, The Journal of Japan Association of Oral Rehabilitation, Nov. 2017.
68.
Takuma Iwasa, S. Afroz, Mio Naritani, 宮城 麻友, Miho Inoue, Masamitsu Ohshima and Yoshizo Matsuka : 神経障害性痛モデルラットに対するボツリヌス毒素の効果, 日本ボツリヌス治療学会, Sep. 2017.
Miho Inoue, Mio Naritani, R. Raju, 宮城 麻友, Y. Zhang, Masamitsu Ohshima, 井上 正久 and Yoshizo Matsuka : 骨髄細胞，骨芽細胞様細胞におけるTNF-α短期間刺激による細胞挙動の解析, 日本硬組織再生生物学会, Aug. 2017.
71.
Takuma Iwasa, Masamitsu Ohshima, Kazuo Okura, Miho Inoue, S. Afroz and Yoshizo Matsuka : 口腔顎顔面痛モデル三叉神経節におけるサイトカイン動態, Japanese Journal of Orofacial Pain, Jul. 2017.
72.
Daiki Teruya, Miho Inoue, Mio Naritani, R. Raju, 宮城 麻友, Masamitsu Ohshima and Yoshizo Matsuka : 骨髄細胞，骨芽細胞様細胞におけるTNF-α短期刺激による細胞学的・組織学的検討, The Journal of the Japan Prosthodontic Society, Jul. 2017.
Mayu Miyagi, Miho Inoue, 福本 美緒 and Yoshizo Matsuka : 炎症環境による歯髄細胞の幹細胞化―歯髄細胞分化に与える TNF-αの影響―, The Journal of Japanese Society of Stomatognathic Function, Apr. 2016.
79.
Yoshizo Matsuka, 大本 勝弘, 岩浅 匠真, Miho Inoue, 福本 美緒, Mayu Miyagi and Kazuo Okura : 知覚神経節における神経伝達物質による痛み情報伝達の可能性, The Journal of Japanese Society of Stomatognathic Function, Apr. 2016.
Et cetera, Workshop:
1.
Raju Resmi, Masamitsu Ohshima, Tsuyoshi Morita, Yan Huijiao, Miho Inoue, Otto Baba, Inoue Masahisa and Yoshizo Matsuka : Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet, 発生・再生・遺伝クラスター ミニリトリート, Feb. 2020.
2.
Resmi Raju, Miho Inoue, M. Naritani, Mayu Miyagi, Yuehui Zhang, Masamitsu Oshima, Masahisa Inoue and Yoshizo Matsuka : Cellular kinetics of bone marrow derived mesenchymal cells by short-term TNF-alpha stimulation, Tokushima University Bioscience Retreat, Sep. 2017.
3.
S. Afroz, Iwasa Takuma, Oshima Masamitsu, Rieko Arakaki, Miho Inoue, Naozumi Ishimaru and Yoshizo Matsuka : Cytokine release from satellite glial cells of trigeminal ganglia, Tokushima University Bioscience Retreat, Sep. 2017.
Report:
1.
Maki Hosoki, Yoshizo Matsuka, Toyoko Tajima, Mayu Miyagi, Miho Inoue, 小澤 彩 and 新開 瑞希 : ピアスによる金属アレルギーのリスクの情報発信と装飾品の品質表示確立に向けた調査研究, Mar. 2024.
Establishment of the new diagnosis biomarkers for metal allergy by analyzing peripheral blood miRNA expression (Project/Area Number: 20K10038 )
Development of a bio-artificial tooth root based on the mechanism for periodontal tissue formation and maturation by using occlusal stress. (Project/Area Number: 19K10208 )
Investigation of mechanism for anti DKK-1 antibody possessing bone formation (Project/Area Number: 17K11753 )
Development of a novel therapy based on stem cell conversion of dental pulp cells (Project/Area Number: 16K11600 )
Metal allergy; What is the difference between sensitization and onset? -Identification of specific genes- (Project/Area Number: 16K11599 )
The development of new baiomaterial promoted regeneration of a dental pulp and odontoblast. (Project/Area Number: 15H06450 )
Molecular biological analysis of oral bacterial population dynamics (Project/Area Number: 10671699 )
Development of inhibitors for a angiogenesis factor CTGF and its application for angiogenetic diseases (Project/Area Number: 10557165 )
Molecular cloning of the receptors for chondrosarcoma-derived chondrocyte growth factor Ecogenin/CTGF (Project/Area Number: 09671893 )
Ecological analysis of Helicobacter pylori in oral cavities (Project/Area Number: 08672069 )