徳島大学 / 教育研究者総覧 --- 和田直樹

個人のホームページ

https://researchmap.jp/read0147916 (researchmap)

専門分野

○	ライフサイエンス (Life sciences) [システムゲノム科学 (Systems genomics)]
○	ライフサイエンス (Life sciences) [応用分子細胞生物学 (Applied molecular and cellular biology)]
○	環境・農学 (Environmental science/Agricultural science) [環境農学 (Environmental agriculture)]
○	ライフサイエンス (Life sciences) [細胞生物学 (Cell biology)]
○	ライフサイエンス (Life sciences) [分子生物学 (Molecular biology)]

研究テーマ

著書・論文

著書:

1.	和田直樹, 刑部祐里子, 刑部敬史 : 新規CRISPR技術を活用したゲノム編集ツールについて, 技術情報協会, 2023年8月.
2.	Naoki Wada, Keishi Osakabe and Yuriko Osakabe : Type I-D CRISPR system-mediated genome editing in plants, Humana New York, 2023. (出版サイトへのリンク) ● Publication site (DOI): 10.1007/978-1-0716-3131-7_2 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1007/978-1-0716-3131-7_2 (DOI: 10.1007/978-1-0716-3131-7_2)
3.	和田直樹, 刑部敬史, 刑部祐里子 : 新規ゲノム編集技術開発による植物細胞の機能改変, 株式会社技術情報協会, 2022年11月.
4.	Naoki Wada, Tomoko Miyaji, Chihiro Abe-Hara, Keishi Osakabe and Yuriko Osakabe : CRISPR/Cas9 tools for multiplex genome editing in crops, Springer, Singapore, Aug. 2022. (出版サイトへのリンク) ● Publication site (DOI): 10.1007/978-981-19-0600-8_4 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1007/978-981-19-0600-8_4 (DOI: 10.1007/978-981-19-0600-8_4)
5.	Naoki Wada, Yuriko Osakabe and Keishi Osakabe : Plant Genome Editing, CAB International, 2022. (出版サイトへのリンク) ● Publication site (DOI): 10.1079/9781789247534.0015 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1079/9781789247534.0015 (DOI: 10.1079/9781789247534.0015)
6.	和田直樹, 刑部祐里子, 刑部敬史 : 第9章高等動植物に利用可能な新規ゲノム編集ツールの開発, 株式会社シーエムシー出版, 2021年.
7.	刑部敬史, 和田直樹 : 第2章DNA編集技術 1. CRISPR/Casによるゲノム編集技術-総論, 株式会社エヌ・ティー・エス, 2021年.
8.	Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui and Mitsuo Oshimura : Production of a Human Cell line with a Plant Chromosome., Humana Press, 2018. (出版サイトへのリンク) ● Publication site (DOI): 10.1007/978-1-4939-7795-6_16 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85047020928 (DOI: 10.1007/978-1-4939-7795-6_16, Elsevier: Scopus)
9.	Naoki Wada, Naruemon Khemkladngoen, Joyce A Cartagena and Kiichi Fukui : Bioactive beads-mediated transformation of plants with large DNA fragments., Humana Press, 2012.
10.	Khemkladngoen Naruemon, Naoki Wada, Suguru Tsuchimoro, Joyce A Cartagene, Shin'ichiro Kajiyama and Kiichi Fukui : Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes, with special reference to bead-production methodology., InTech, USA, 2012.
11.	Naoki Wada, Shin'ichiro Kajiyama, Naruemon Khemkladngoen and Kiichi Fukui : A novel gene delivery system in plants with calcium alginate micro-beads., Wiley-Blackwell Publishers, 2011.

学術論文(審査論文):

1.	Ismail A. Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui : Adenine sulfate and glutamine enhanced shoots multiplication in Jatropha curcas L. a potent biofuel plant, African Journal of Biotechnology, Vol.20, No.9, 383-388, 2021. (出版サイトへのリンク) ● Publication site (DOI): 10.5897/AJB2021.17364 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.5897/AJB2021.17364 (DOI: 10.5897/AJB2021.17364)
2.	Keishi Osakabe, Naoki Wada, Emi Murakami, Naoyuki Miyashita and Yuriko Osakabe : Genome editing in mammalian cells using the CRISPR type I-D nuclease, Nucleic Acids Research, 2021. (徳島大学機関リポジトリ) ● Metadata: 116611 (出版サイトへのリンク) ● Publication site (DOI): 10.1093/nar/gkab348 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1093/nar/gkab348 (徳島大学機関リポジトリ: 116611, DOI: 10.1093/nar/gkab348)
3.	YengMun Liaw, Yikun Liu, CheeHow Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel and Nobuko. Ohmido : Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a HumanArabidopsis Hybrid Cell Line, International Journal of Molecular Sciences, Vol.22, No.11, 5426, 2021. (徳島大学機関リポジトリ) ● Metadata: 116539 (出版サイトへのリンク) ● Publication site (DOI): 10.3390/ijms22115426 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.3390/ijms22115426 (徳島大学機関リポジトリ: 116539, DOI: 10.3390/ijms22115426)
4.	Yikun Liu, Yeng Mun Liaw, Chee How Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel and Nobuko Ohmido : Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines, Scientific Reports, 2021. (徳島大学機関リポジトリ) ● Metadata: 116632 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s41598-021-86130-4 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-021-86130-4 (徳島大学機関リポジトリ: 116632, DOI: 10.1038/s41598-021-86130-4)
5.	Chihiro Abe-Hara, Kohji Yamada, Naoki Wada, Risa Ueta, Ryosuke Hashimoto, Keishi Osakabe and Yuriko Osakabe : Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars, Frontiers in Plant Science, 2021. (徳島大学機関リポジトリ) ● Metadata: 116525 (出版サイトへのリンク) ● Publication site (DOI): 10.3389/fpls.2021.627832 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.3389/fpls.2021.627832 (徳島大学機関リポジトリ: 116525, DOI: 10.3389/fpls.2021.627832)
6.	Keishi Osakabe, Naoki Wada, Miyaji Tomoko, Murakami Emi, Marui Kazuya, Ueta Risa, Hashimoto Ryosuke, Abe-Hara Chihiro, Kong Bihe, Yano Kentaro and Yuriko Osakabe : Genome editing in plants using CRISPR type I-D nuclease., Communications Biology, Vol.3, 648, 2020. (徳島大学機関リポジトリ) ● Metadata: 115886 (出版サイトへのリンク) ● Publication site (DOI): 10.1038/s42003-020-01366-6 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1038/s42003-020-01366-6 (徳島大学機関リポジトリ: 115886, DOI: 10.1038/s42003-020-01366-6)
7.	Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, HIroe Sakai, Naoki Wada, Arefeh Alipour, Tomohiro Sasai, Atsushi Tunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, HIdeaki Hirakawa, Victor Pecina-Quintero, Alfredo Zamarripa and Kiichi Fukui : Association Study of Seed-Yield Related Traits for Jatropha curcas L. in Mexico, Tropical Agriculture and Development, Vol.62, No.2, 68-77, 2018. (出版サイトへのリンク) ● Publication site (DOI): 10.11248/jsta.62.68 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.11248/jsta.62.68 (DOI: 10.11248/jsta.62.68)
8.	Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, Hideki Hirakawa, P Victor Quintero, Alfredo Zamarripa, Primitivo Santos, Adel Hegazy, M Abdalla Ali and Kiichi Fukui : Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World., Frontiers in Plant Science, Vol.8, No.1539, 2017. (要約) L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers. We found that central Chiapas of Mexico possesses the most diverse genetic resources, and the Chiapas Central Depression could be the center of origin. We identified three genetic groups in Mesoamerica, whose distribution revealed a distinct geographic cline. One of them consists mainly of accessions from central Chiapas. This suggests that it represents the original genetic group. We found two Veracruz accessions in another group, whose ancestors might be shipped from Port of Veracruz to the Old World, to be the source of all African and Asian Jatropha. Our results suggest the human selection that caused low productivity in Africa and Asia, and also breeding strategies to improve African and Asian Jatropha. Cultivars improved in the productivity will contribute to expand mass commercial cultivation of Jatropha in Africa and Asia to increase biofuel production, and finally will support in the battle against the climate change. (徳島大学機関リポジトリ) ● Metadata: 114486 (出版サイトへのリンク) ● Publication site (DOI): 10.3389/fpls.2017.01539 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28936216 ● Search Scopus @ Elsevier (PMID): 28936216 ● Search Scopus @ Elsevier (DOI): 10.3389/fpls.2017.01539 (徳島大学機関リポジトリ: 114486, DOI: 10.3389/fpls.2017.01539, PubMed: 28936216)
9.	Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukuui : Agrobacterium-Mediated Transformation of Jojoba [Simmondsia chinensis (Link) Schneider], Tropical Agriculture and Development, Vol.61, No.1, 23-31, 2017. (出版サイトへのリンク) ● Publication site (DOI): 10.11248/jsta.61.23 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.11248/jsta.61.23 (DOI: 10.11248/jsta.61.23)
10.	Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui : High-frequency Shoots Regeneration of an Oil Crop, Simmondsia chinensis (Link) Schneider Using Axillary Buds., Tropical Agriculture and Development, Vol.61, No.1, 15-22, 2017. (出版サイトへのリンク) ● Publication site (DOI): 10.11248/jsta.61.15 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.11248/jsta.61.15 (DOI: 10.11248/jsta.61.15)
11.	Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui and Mitsuo Oshimura : Maintenance and Function of a Plant Chromosome in Human Cells., ACS Synthetic Biology, Vol.6, No.2, 301-310, 2016. (要約) Replication, segregation, gene expression, and inheritance are essential features of all eukaryotic chromosomes. To delineate the extent of conservation of chromosome functions between humans and plants during evolutionary history, we have generated the first human cell line containing an Arabidopsis chromosome. The Arabidopsis chromosome was mitotically stable in hybrid cells following cell division, and initially existed as a translocated chromosome. During culture, the translocated chromosomes then converted to two types of independent plant chromosomes without human DNA sequences, with reproducibility. One pair of localization signals of CENP-A, a marker of functional centromeres was detected in the Arabidopsis genomic region in independent plant chromosomes. These results suggest that the chromosome maintenance system was conserved between human and plants. Furthermore, the expression of plant endogenous genes was observed in the hybrid cells, implicating that the plant chromosomal region existed as euchromatin in a human cell background and the gene expression system is conserved between two organisms. The present study suggests that the essential chromosome functions are conserved between evolutionarily distinct organisms such as humans and plants. Systematic analyses of hybrid cells may lead to the production of a shuttle vector between animal and plant, and a platform for the genome writing. (キーワード) Base Sequence / Cell Fusion / Cell Line, Tumor / Centromere / Chromosomes, Plant / DNA / Gene Expression / Humans / Hybrid Cells / Reproducibility of Results (出版サイトへのリンク) ● Publication site (DOI): 10.1021/acssynbio.6b00180 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27696824 ● Search Scopus @ Elsevier (PMID): 27696824 ● Search Scopus @ Elsevier (DOI): 10.1021/acssynbio.6b00180 (DOI: 10.1021/acssynbio.6b00180, PubMed: 27696824)
12.	Misa Fujiwara, Go Suzuki, Daiki Kudo, Haruna Oba, Yukio Wada, Hideo Wada, Naoki Wada, Sadequr Rahman, Kiichi Fukui and Yasuhiko Mukai : Localization of transgene-derived friabilins in rice endosperm cells, Plant Biotechnology, Vol.31, No.1, 67-70, 2014. (出版サイトへのリンク) ● Publication site (DOI): 10.5511/plantbiotechnology.13.1028a (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.5511/plantbiotechnology.13.1028a (DOI: 10.5511/plantbiotechnology.13.1028a)
13.	Suguru Tsuchimoto, Joyce A Cartagena, Khemkladngoen Naruemon, Singkaravanit Suthitar, Tsutomu Kohinata, Naoki Wada, HIroe Sakai, Daisuke Shibata and Kiichi Fukui : Development of transgenic plants in jatropha with drought tolerance, Plant Biotechnology, Vol.29, No.2, 137-143, 2012. (出版サイトへのリンク) ● Publication site (DOI): 10.5511/plantbiotechnology.12.0406d (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.5511/plantbiotechnology.12.0406d (DOI: 10.5511/plantbiotechnology.12.0406d)
14.	Shusei Sato, Hideki Hirakawa, Sachiko Isobe, Eigo Fukai, Akiko Watanabe, Midori Kato, Kumiko Kawashima, Chiharu Minami, Akiko Muraki, Naomi Nakazaki, Chika Takahashi, Shinobu Nakayama, Yoshie Kishida, Mitsuyo Kohara, Manabu Yamada, Hisano Tsuruoka, Shigemi Sasamoto, Satoshi Tabata, Tomoyuki Aizu, Atsushi Toyoda, Tadasu Shin-i, Yohei Minakuchi, Yuji Kohara, Asao Fujiyama, Suguru Tsuchimoto, Shin'ichiro Kajiyama, Eri Makigano, Nobuko Ohmido, Nakako Shibagaki, A Joyce Cartagena, Naoki Wada, Tsutomu Kohinata, Alipour Atefeh, Shota Yuasa, Sachihiro Matsunaga and Kiichi Fukui : Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L., DNA Research, Vol.18, No.1, 65-76, 2010. (要約) The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/. (キーワード) Genome, Plant / Jatropha / Plant Proteins / Sequence Analysis, DNA (出版サイトへのリンク) ● Publication site (DOI): 10.1093/dnares/dsq030 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21149391 ● Search Scopus @ Elsevier (PMID): 21149391 ● Search Scopus @ Elsevier (DOI): 10.1093/dnares/dsq030 (DOI: 10.1093/dnares/dsq030, PubMed: 21149391)
15.	Naoki Wada, Shin'ichiro Kajiyama, Joyce A Cartagena, Linyen Lin, Yukio Akiyama, Motoyasu Otani, Go Suzuki, Yasuhiko Mukai, Noriaki Aoki and Kiichi Fukui : The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant, Journal of Cereal Science, Vol.51, No.2, 182-188, 2010. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.jcs.2009.11.010 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jcs.2009.11.010 (DOI: 10.1016/j.jcs.2009.11.010)
16.	Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Daisuke No, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes., Plant Cell Reports, Vol.28, No.5, 759-768, 2009. (要約) Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T(2) generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously. (キーワード) Chromosomes, Artificial, Bacterial / Cinnamates / DNA, Plant / Gene Expression / Gene Transfer Techniques / Genes, Plant / Genetic Vectors / Hygromycin B / Microspheres / Oryza / Plants, Genetically Modified / Poaceae / Transformation, Genetic / Transgenes (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s00299-009-0678-2 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19214515 ● Search Scopus @ Elsevier (PMID): 19214515 ● Search Scopus @ Elsevier (DOI): 10.1007/s00299-009-0678-2 (DOI: 10.1007/s00299-009-0678-2, PubMed: 19214515)

総説・解説:

1.	和田直樹 : フュージョンでは何が起こっている? 細胞工学の専門家がたどり着いた「受精卵まで戻っている」説, ドラゴンボールオフィシャルサイト, 2023年11月.
2.	Naoki Wada, Keishi Osakabe and Yuriko Osakabe : Genome editing in plants, Gene and Genome Editing, Vol.3-4, 100020, Dec. 2022. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.ggedit.2022.100020 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1016/j.ggedit.2022.100020 (DOI: 10.1016/j.ggedit.2022.100020)
3.	Naoki Wada, Keishi Osakabe and Yuriko Osakabe : Expanding the plant genome editing toolbox with recently developed CRISPR-Cas systems, Plant Physiology, Vol.188, No.4, 1825-1837, Jan. 2022. (要約) Since its first appearance, CRISPR-Cas9 has been developed extensively as a programmable genome-editing tool, opening a new era in plant genome engineering. However, CRISPR-Cas9 still has some drawbacks, such as limitations of the protospacer-adjacent motif (PAM) sequence, target specificity, and the large size of the cas9 gene. To combat invading bacterial phages and plasmid DNAs, bacteria and archaea have diverse and unexplored CRISPR-Cas systems, which have the potential to be developed as a useful genome editing tools. Recently, discovery and characterization of additional CRISPR-Cas systems have been reported. Among them, several CRISPR-Cas systems have been applied successfully to plant and human genome editing. For example, several groups have achieved genome editing using CRISPR-Cas type I-D and type I-E systems, which had never been applied for genome editing previously. In addition to higher specificity and recognition of different PAM sequences, recently developed CRISPR-Cas systems often provide unique characteristics that differ from well-known Cas proteins such as Cas9 and Cas12a. For example, type I CRISPR-Cas10 induces small indels and bi-directional long-range deletions ranging up to 7.2 kb in tomatoes (Solanum lycopersicum L.). Type IV CRISPR-Cas13 targets RNA, not double-strand DNA, enabling highly specific knockdown of target genes. In this article, we review the development of CRISPR-Cas systems, focusing especially on their application to plant genome engineering. Recent CRISPR-Cas tools are helping expand our plant genome engineering toolbox. (キーワード) CRISPR-Cas Systems / Gene Editing / Genome, Plant / Humans / Plants (徳島大学機関リポジトリ) ● Metadata: 117145 (出版サイトへのリンク) ● Publication site (DOI): 10.1093/plphys/kiac027 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35099553 ● Summary page in Scopus @ Elsevier: 2-s2.0-85128159902 (徳島大学機関リポジトリ: 117145, DOI: 10.1093/plphys/kiac027, PubMed: 35099553, Elsevier: Scopus)
4.	Naoki Wada, Ueta Risa, Yuriko Osakabe and Keishi Osakabe : Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering, BMC Plant Biology, Vol.20, No.1, 234, May 2020. (要約) Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) has emerged as a new genome-editing tool, extensively applied in various organisms, including plants. The use of CRISPR/Cas9 allows generating transgene-free genome-edited plants ("null segregants") in a short period of time. In this review, we provide a critical overview of the recent advances in CRISPR/Cas9 derived technologies for inducing mutations at target sites in the genome and controlling the expression of target genes. We highlight the major breakthroughs in applying CRISPR/Cas9 to plant engineering, and challenges toward the production of null segregants. We also provide an update on the efforts of engineering Cas9 proteins, newly discovered Cas9 variants, and novel CRISPR/Cas systems for use in plants. The application of CRISPR/Cas9 and related technologies in plant engineering will not only facilitate molecular breeding of crop plants but also accelerate progress in basic research. (徳島大学機関リポジトリ) ● Metadata: 115250 (出版サイトへのリンク) ● Publication site (DOI): 10.1186/s12870-020-02385-5 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32450802 ● Summary page in Scopus @ Elsevier: 2-s2.0-85085388267 (徳島大学機関リポジトリ: 115250, DOI: 10.1186/s12870-020-02385-5, PubMed: 32450802, Elsevier: Scopus)
5.	和田直樹 : ヒトと植物細胞の細胞融合:16億年の時を超えた再会, 現代化学, No.5, 41-43, 2017年.
6.	和田直樹 : 不毛な大地で油を作る植物たち, 生物工学会誌, Vol.93, No.3, 156, 2015年.

国際会議:

1.	Kurihara Satoshi, Naoki Wada, Murakami Emi, Marui Kazuya, Yuriko Osakabe and Keishi Osakabe : Establishment of episomal vector-based CRISPR-Cas type I-D system for efficient genome editing in human cells, Genome Engineering:CRISPR Frontiers, Cold Spring Harbor Laboratory, NY, USA, Aug. 2024.
2.	Naoki Wada, Murakami Emi, Marui Kazuya, Yuriko Osakabe and Keishi Osakabe : Efficient gene knockout using CRISPR-Cas type I-D combined with TriFC system in diploid human cells, Genome Engineering:CRISPR Frontiers, Cold Spring Harbor Laboratory, NY, USA, Aug. 2024.
3.	Naoki Wada, Murakami Emi, Marui Kazuya, Yuriko Osakabe and Keishi Osakabe : Development of a highly efficient genome editing tool using Type I-D CRISPR-Cas, Keystone Symposia on Precision Genome Engineering, Jan. 2024.
4.	Naoki Wada, Murakami Emi, Marui Kazuya, Hashimoto Ryosuke, Osakabe Yuriko and Osakabe Keishi : Development and characterization of a novel genome editing tool, the TiD system, Keystone symposia on Molecular and Cellular Biology Plant Genome Engineering: From lab to Field, Mar. 2021.
5.	YengMun Liaw, Yikun Liu, Petr Cápal, Jaroslav Doležel, CheeHow Teo, Naoki Wada, Kiichi Fukui and Nobuko Ohmido : Genetics and Epigenetics of Plant Chromosomes in a Human-plant hybrid cell line, Asia Pacific Chromosome Colloquium, Busan, Korea, Nov. 2020.
6.	Keishi Osakabe, Naoki Wada, Marui Kazuya, Murakami Emi, Ueta Risa, Hashimoto Ryosuke, Hara Chihiro, Miyaji Tomoko and Yuriko Osakabe : Genome editing in plants by using a novel genome editing tool TiD, Frontiers in Genome Engineering 2019, Kobe Convention Center, Nov. 25-27, 2019, Nov. 2019.
7.	Naoki Wada, Murakami Emi, Hashimoto Ryosuke, Yuriko Osakabe and Keishi Osakabe : DEVELOPMENT OF A NOVEL GENOME EDITING TOOL, TID SYSTEM, FOR MAMMALIAN GENOME ENGINEERING, Frontiers in Genome Engineering 2019, Kobe Convention Center, Nov. 25-27, 2019, Nov. 2019.
8.	Naoki Wada : Maintenance and function of a plant chromosome in human cells, The 11th International Symposium Exploring the Global Sustainability, Mar. 2018.
9.	Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui : Developing industrial oil-seed trees for drylands, The 10th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
10.	Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Hiroe Sakai, Naoki Wada and Kiichi Fukui : Genetic diversity and the seed-trait variation in jojoba accessions from America, Africa and Asia, Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
11.	Naoki Wada, Yutaka Minote, Hiroe Sakai, Suguru Tsuchimoto, Shin"ichiro Kajiyama and Kiichi Fukui : Development of in planta liquid wax ester production system by targeting the biosynthetic enzymes into different organelles, The 10th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2017.
12.	Naoki Wada : A partial hybrid cell line between human and plants, Interdisciplinary German-Japanese symposium iJaDe 2016 in IPK Gatersleben, May 2016.
13.	Naoki Wada : A partial hybrid cell line between human and plants, Interdisciplinary German-Japanese symposium iJaDe 2016 in Technische Universität Dresden, May 2016.
14.	Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Hisashi Tomemori, Satoshi Tabata, Shusei Sato, Hideki Hirakawa, Victor P Quintero, Alfredo Zamarripa and Kiichi Fukui : Association analysis of yield-related traits in Jatropha curcas L, The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
15.	Ismail A Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui : An efficient in vitro regeneration and transformation system of Jatropha curcas L., The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
16.	Chiaki Katsuura, Naoki Wada, Hiroe Sakai, Suguru Tsuchimoto and Kiichi Fukui : Screening and identification of effective genes for reinforcing drought tolerance of Jatropha curcas L, The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land, Mar. 2016.
17.	Suguru Tsuchimoto, Ismail A Mohammed, Hayiyan Li, Minote Yutaka, Kyuya Harada, Hiroe Sakai, Naoki Wada, Adel Hegazy, Tsutomu Fushimi and Kiichi Fukui : Jojoba: Research and practice, The 9th International symposium Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land-, Mar. 2016.
18.	Naoki Wada : A hybrid cell line between human and plant, The 2nd UK-Japan Chromosome Workshop, May 2015.
19.	Naoki Wada, Yasuhiro Kazuki, Kiichi Fukui and MItsuo Oshimura : A hybrid cell line between human and plant, The 5th Asian Chromosome Colloquium, May 2015.
20.	Naoki Wada : Application of plant biotechnology to Shimmondsia chinesis (jojoba) plants, The 8th International Symposium Exploring the global sustainability Advances in Plant Biotechnology for Agriculture in Semi-arid land, Mar. 2015.
21.	Naoki Wada : Importance of jojoba (Shimondsia chinensis) as a strategic bioresource, Genetic Engineering and Biotechnology Research Institute (GEBRI) and Tissue Culture and Genetic Engineering Center Seminar in University of Sadat City, Egypt, Oct. 2014.
22.	Suguru Tsuchimoro, Joyce A Cartagena, Naruemon Khemkladngoen, Suithitar Singkaravanit, Tsutomu Kohinata, Naoki Wada, Hiroe Sakai, Yoshihiko Morishita, Hideyuki Suzuki, Daisuke Shibata and Kiichi Fukui : DEVELOPMENT OF JATROPHA TRANSGENIC PLANTS WITH DROUGHT TOLERANCE, International Conference for Bioenergy and Biomass Utilization, Aug. 2012.
23.	Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Joyce A Cartagena, Linyen Lin, Noriaki Aoki, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii gene, Japanese-German JSPS and DFG-funded workshop: Frontiers of Plant Chromosome Research: Centromeres and Artificial Chromosomes, Nov. 2011.
24.	Naoki Wada, Joyce A Cartagena, Naruemon Khemkladngoen, Tsutomu Kohinata and Kiichi Fukui : Transformation of Jatropha curcas with Phosphopantetheine Adenylyltrasnferaes (PPAT) gene to enhance the drought stress tolerance, Symposium on Jatropha Research: Updates and Future Perspectives, Jan. 2011.
25.	Naoki Wada : 油糧植物ジャトロファからの環境問題へのアプローチ, 中日学者草原生態問題学術座談会 in 内モンゴル自治区社会科学研究院, Aug. 2010.
26.	Naoki Wada, Shin'ichiro Kajiyama, Joyce A Cartagena, Yukio AKiyama, Lin Linyen, Noriaki Aoki, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Bioactive Beads-Mediated Transformation Of Rice With Large DNA Fragments Containing Aegilops tauschii Hardness Genes, Plant and Animal Genome XVIII Conference, Feb. 2010.
27.	Kiichi Fukui, Naoki Wada and Susumu Uchiyama : Bioactive beads-mediated transformation of plants with large DNA fragments, Plant and Animal Genome XX Conference, Jan. 2010.
28.	Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Transformation of rice with large DNA fr agments using a bio-active beads metho, 8th Osaka University-KAIST Graduate Students Symposium in Biotechnology, Feb. 2009.
29.	Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Motoyasu Otani, Takiko Shimada, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Transformation of rice with high molecular weight DNAs using bio-active beads method, The 3rd Asian Chromosome Colloquium, Dec. 2008.
30.	Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai and Kiichi Fukui : Development of a New Plant Transformation System with Large DNAs using Bioactive Beads Method, The 1st international global COE symposium on Bio-Enviromental Chemistry, Jan. 2008.
31.	Kiichi Fukui, Naoki Wada, Naruemon Khemkladngoen and Shin'ichiro Kajiyama : A novel gene delivery system in plants with calcium alginate micro-beads, Plant Transformation technologies, Feb. 2007.
32.	Naoki Wada, Koichi Ito, Naoko Nose, Yukio Akiyama, Shigeki Kawakami, Sachihiro Matsunaga, Motoyasu Otani, Go Suzuki, Yasuhiko Mukai, Takiko Shimada and Kiichi Fukui : Transformation of Rice Plants Using Bio-Active Beads with BACs, Plant & Animal Genome XIV, Jan. 2006.

国内講演発表:

1.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : TiD-X を用いたヒト2 倍体細胞での遺伝子ノックアウト, 日本ゲノム編集学会第9回大会, 2024年6月.
2.	渡邊龍弥, 城所聡, 和田直樹, 刑部敬史, 刑部祐里子 : Development of transcriptional control tools using type I-D CRISPR-Cas system, 日本ゲノム編集学会第9回大会, 2024年6月.
3.	室本翔太, 阿江祐迪, 丸井和也, 和田直樹, 刑部敬史, 刑部祐里子 : 改良型Type I-D CRISPR-Cas (TiD-X) を用いたイネ遺伝子改変技術の確立, 日本ゲノム編集学会第9回大会, 2024年6月.
4.	小島龍弥, 松永朋子, 墨谷暢子, 和田直樹, 刑部敬史, 松永幸大 : CHO細胞におけるクロロフィル合成経路の構築によるクロロフィルaの生合成, 日本メンデル協会第一回大会, 2024年6月.
5.	室本翔太, 阿江祐迪, 丸井和也, 和田直樹, 刑部敬史, 刑部祐里子 : 改良型 Type I-D CRISPR-Cas によるイネ高効率ゲノム編集, 第65回日本植物生理学会年会, 2024年3月.
6.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : 新Type I-D CRISPR-Cas (TiD-X)を用いた高効率遺伝子ノックアウト, 第46回日本分子生物学会年会, 2023年12月.
7.	渡邊龍弥, 城所聡, 和田直樹, 刑部敬史, 刑部祐里子 : TiDを用いた転写制御ツールの開発, 第46回日本分子生物学会年会, 2023年12月.
8.	栗原慧士, 和田直樹, 丸井和也, 村上愛美, 刑部祐里子, 刑部敬史 : 新規ゲノム編集技術TiDにおけるエピソーマルベクターの利用と効率化, 第46回日本分子生物学会年会, 2023年12月.
9.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : 新規Type I-D CRISPR-Cas (TiD-X)を用いた高効率なヒトゲノム編集., 第75回日本生物工学会大会, 2023年9月.
10.	栗原慧士, 和田直樹, 丸井和也, 村上愛美, 刑部祐里子, 刑部敬史 : エピソーマルベクターを用いた新規ゲノム編集技術TiDによる高効率ゲノム編集法の確立, 日本ゲノム編集学会第8回大会, 2023年6月.
11.	渡邊龍弥, 城所聡, 和田直樹, 近藤京子, 刑部敬史, 刑部祐里子 : TiDを用いた新規転写制御ツールの開発, 日本ゲノム編集学会第8回大会, 2023年6月.
12.	刑部祐里子, 城所聡, 野口聡子, 近藤京子, 大濱直彦, 和田直樹, 刑部敬史 : Type I-D CRISPR-Cas, TiDによるエクソンスキッピング療法のモデル検証, 日本ゲノム編集学会第8回大会, 2023年6月.
13.	和田直樹 : 植物におけるゲノム編集技術の開発動向, 日本ゲノム編集学会第8回大会, 2023年6月.
14.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : 新規Type I-D CRISPR-Cas (TiD-X)を利用した高効率ゲノム編集系の確立, 日本ゲノム編集学会第8回大会, 2023年6月.
15.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : CRISPR-Cas type I-D においてCas11dがヒト細胞でのゲノム編集へ与える影響の解析, 第45回日本分子生物学会年会, 2022年12月.
16.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : Cas11dの設計・発現による新規ゲノム編集技術CRISPR-Cas type I-D (TiD)の高効率化, 創立100周年記念第74回日本生物工学会大会, 2022年10月.
17.	和田直樹, 村上愛美, 丸井和也, 刑部祐里子, 刑部敬史 : Cas11dを用いた新規ゲノム編集ツールTiDの改良, 日本ゲノム編集学会第7回大会, 2022年6月.
18.	和田直樹, 村上愛美, 刑部祐里子, 刑部敬史 : FACSを用いた新規ゲノム編集ツールTiDによる効率的な遺伝子ノックアウト作出方法の開発, 第44回日本分子生物学会年会, 2021年12月.
19.	和田直樹, 村上愛美, 丸井和也, 宮下尚之, 刑部祐里子, 刑部敬史 : 新規ゲノム編集ツールTiDを用いたヒト細胞でのゲノム編集, 第73回日本生物工学会大会, 2021年10月.
20.	近江戸伸子, Liaw Mun Yen, Liu Yukun, Tel How Chee, Capal Petr, 和田直樹, 福井希一, Jaroslav Doležel : 植物染色体は動物細胞の中でどのように適応していくのか?, 一般財団法人染色体学会第72回(2021年度)年会, 2021年9月.
21.	和田直樹, 宮地朋子, 村上愛美, 丸井和也, 上田梨紗, 橋本諒典, 阿部-原千尋, Kong Bihe, 矢野健太郎, 刑部祐里子, 刑部敬史 : 新規ゲノム編集ツールTiDシステムを用いた植物のゲノム編集, 第62回日本植物生理学会年会, 2021年3月.
22.	和田直樹, 村上愛美, 宮下尚之, 刑部祐里子, 刑部敬史 : 新しいゲノム編集ツールTiDシステムの開発, 第43回日本分子生物学会年会, 2020年12月.
23.	上田梨紗, 吉良望, 原千尋, 宮地朋子, 和田直樹, 刑部祐里子, 刑部敬史 : in planta-regeneration法におけるdCas9-転写活性化ベクターを用いた遺伝子発現制御システムの開発, 第61回日本植物生理学会年会, 2020年3月.
24.	Liu YiKun, Liaw Mun Yeng, Cápal Petr, Dolezel Jaroslav, Teo How Chee, Naoki Wada, Fukui Kiichi and Ohmido Nobuko : Reconstruction of Arabidopsis chromosome in human-plant hybrid cell, 染色体学会第70回年会, Sep. 2019.
25.	上田梨紗, 吉良望, 吉岡里香, 宮地朋子, 和田直樹, 刑部祐里子, 刑部敬史 : CRISPR/dCas9を利用した植物遺伝子発現制御システムの開発, 第37回日本植物細胞分子生物学会, 2019年9月.
26.	和田直樹, 村上愛美, 刑部祐里子, 刑部敬史 : Development of a highly sensitive guide RNA evaluation system using Nano Luciferase, The 12th International Symposium Exploring the Global Sustainability, Kindai University, 201985, 2019年8月.
27.	和田直樹, 村上愛美, 刑部祐里子, 刑部敬史 : Nano Luciferaseを用いた高感度ガイドRNA評価システムの開発, 日本ゲノム編集学会第4回大会, 2019年6月.
28.	上田梨紗, 宮地朋子, 和田直樹, 刑部祐里子, 刑部敬史 : CRISPR/dCas9を利用した植物遺伝子発現制御システムの開発, 第60回日本植物生理学会年会, 2019年3月.
29.	Matsunaga Sachihiro, Ishiyama Sumire, 和田直樹, Matsunaga Tomoko : Construction of hybrid cells between algae and human cultured cells toward creation of planimal cells, 第30回日本植物形態学会, 2018年9月.
30.	和田直樹, 香月康宏, 香月加奈子, 井上敏昭, 刑部敬史, 福井希一, 押村光雄 : 植物/ヒト雑種細胞における植物染色体の挙動と遺伝子発現, 第59回日本植物生理学会, 2018年3月.
31.	和田直樹 : ヒトと植物細胞の部分的な融合細胞株の樹立 (招待講演), 第38回日本動物細胞工学会シンポジウム, 2018年3月.
32.	和田直樹 : ヒトと植物細胞の部分的な融合細胞株の樹立—進化を超えた染色体機能保存性の解明を目指してー (招待講演), 第5回細胞凝集研究会, 2017年11月.
33.	和田直樹, 香月康宏, 香月加奈子, 井上敏昭, 福井希一, 押村光雄 : ヒト細胞における植物染色体の維持と機能, 第39回日本分子生物学会, 2016年12月.
34.	Haiyan Li, 土本卓, 原田久也, 山崎将紀, 酒井啓江, Naoki Wada, Alipour Atefeh, 笹井知博, 恒川篤史, 辻本壽, 安藤孝之, 留森寿士, 佐藤修正, 平川英樹, Wuintero Pecina Victor, Zamarripa Alfredo, Santos Primitivo, Hegazy Adel, Ali Mohamed Abdalla and 福井希一 : DNA マーカーを用いた燃料作物ジャトロファの起源中心の遺伝学的解析, 第130回日本育種学会, Sep. 2016.
35.	和田直樹 : 乾燥ストレス耐性を付与した油糧ジャトロファの創出, VBL異分野融合研究会, 2010年11月.
36.	和田直樹, 梶山慎一郎, Joyce A Cartagena, 林麟晏, 秋山征夫, 大谷基泰, 鈴木剛, 向井康比己, 青木法明, 内山進, 松永幸大, 福井希一 : バイオアクティブビーズ法を用いた巨大DNA導入による新規形質転換イネの作出, H20年度先端科学イノベーションセンター公開成果発表会, 2010年3月.
37.	和田直樹, 梶山慎一郎, 秋山征夫, 大谷基泰, 島田多喜子, 鈴木剛, 向井康比己, 福井希一 : バイオアクティブビーズ法によるハードネス遺伝子群導入イネの作出, 第114回日本育種学会, 2008年10月.
38.	和田直樹, 池内智彦, 梶山慎一郎, 大谷基泰, 鈴木剛, 向井康比己, 島田多喜子, 福井希一 : バイオアクティブビーズ法によるコムギ有用遺伝子群を含む巨大DNAのイネへの導入, 大阪大学イノベーションセミナー2007, 2007年10月.
39.	和田直樹, 秋山征夫, 大谷基泰, 鈴木剛, 向井康比己, 島田多喜子, 福井希一 : バイオアクティブビーズ法によるコムギ有用遺伝子群を含む巨大DNAのイネへの導入, 第110回日本育種学会, 2006年9月.
40.	和田直樹, 秋山征夫, 大谷基泰, 鈴木剛, 向井康比己, 島田多喜子, 福井希一 : バイオアクティブビーズ法を用いた巨大遺伝子群の導入による新型有用米の開発, VBL公開成果発表会, 2006年3月.
41.	和田直樹, 川上茂樹, 松永幸大, 向井康比己, 島田多喜子, 福井希一 : バイオアクティブビーズ法を用いた新規植物「パンコメ」の開発, 第57回日本生物工学会, 2005年11月.
42.	和田直樹, 川上茂樹, 松永幸大, 向井廉比己, 島田多喜子, 福井希一 : バイオアクティブビーズ法を用いた新規植物「パンコメ」の開発, 大阪大学イノベーションセミナー2005, 2005年10月.
43.	和田直樹, 福井希一 : バイオアクティブビーズ法を用いた新型食品の開発, 第5回阪大フロンティア・シンポジウム・2004年度研究成果展示発表会, 2005年5月.
44.	和田直樹, 川上茂樹, 松永幸大, 内山進, 福井希一 : バイオビーズ法を用いた新規植物"パンコメ"の作出, 2004年度先端科学イノベーションセンターVBL部門公開成果発表会, 2005年3月.
45.	和田直樹, 川上茂樹, 向井康比己, 福井希一 : バイオビーズ法を用いた新規植物「パンコメ」の作出, 近畿地域アグリビジネス創出フェア, 2004年12月.
46.	和田直樹, 川上茂樹, 向井廉比己, 福井希一 : バイオビーズ法を用いた新規植物「パンコメ」の作出, バイオメディカルベンチャー創出を目指した若手交流会, 2004年10月.
47.	和田直樹, 松永幸大, 内山進, 福井希一 : バイオアクティブビーズを用いた遺伝子デリバリー, 第一回イノベーション2004, 2004年10月.
48.	和田直樹, 福井希一 : 遺伝子改変による新型食品の開発, イノベーション・ジャパン2004, 2004年9月.

特許:

1.	刑部敬史, 刑部祐里子, 和田直樹 : CRISPRタイプI-Dを利用した標的ヌクレオチド配列改変技術, 特願PCT/JP2021/037194 (2021年10月), 特開WO/2022/075419 (2022年4月), .
2.	刑部敬史, 刑部祐里子, 和田直樹 : CRISPRタイプI-Dシステムを利用した標的配列改変技術, 特願PCT/JP2020/011283 (2019年3月), 特開WO/2020/184723 (2020年9月), .
3.	福井希一, ジョイスカルタヘナ, 和田直樹, 小日向務, 湯塩泰久, 池口直樹, 田畑哲之 : ヤトロファ由来のPPATをコードするポリヌクレオチド及びその利用, 特願2010-27361 (2012年12月), 特開2012-120478(P2012-120478A), 特許第603028号.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

植物ゲノムへのtype I-D CRISPR-Cas変異導入機構の解明と応用 (研究課題/領域番号: 23K23566 )
高特異性かつ高効率な改良版国産ゲノム編集技術TiDの開発 (研究課題/領域番号: 22K06192 )
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