徳島大学 / 教育研究者総覧 --- 中井綾

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徳島大学 ⟩ バイオイノベーション研究所 ⟩

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著書・論文

学術論文(審査論文):

1.	Qingyi Lin, Takeshige Otoi, Setyo Oky Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Zhao Namula, Maki Hirata, Aya Nakai, Yuichiro Nakayama, Megumi Nagahara and Fuminori Tanihara : Synergistic enhancement of clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated protein 9 -mediated gene editing in porcine zygotes through combined lipofection and electroporation of cationic lipid-packaged ribonucleoproteins, Veterinary World, 18, 12, 3806-3814, 2025. (キーワード) cationic lipid / CRISPR/Cas9 / electroporation / genome-editing efficiency / lipofectamine / porcine zygote / xenotransplantation (出版サイトへのリンク) ● Publication site (DOI): 10.14202/vetworld.2025.3806-3814 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-105024471085 (DOI: 10.14202/vetworld.2025.3806-3814, Elsevier: Scopus)
2.	Zhao Namula, Takeshige Otoi, Theerawat Tharasanit, Kaywalee Chatdarong, Megumi Nagahara, Setyo Oky Widodo, Aya Nakai, Thi Suong Nguyen, Yuichiro Nakayama, Maki Hirata and Fuminori Tanihara : Establishment of a Single-Oocyte Culture System for Pigs and Its Validation Using Curcumin as a Model Antioxidant for Oocyte Maturation, Animals : An Open Access Journal from MDPI, 15, 22, 2025. (キーワード) curcumin / meiotic competence / microdroplet / porcine oocyte / single culture system (出版サイトへのリンク) ● Publication site (DOI): 10.3390/ani15223295 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-105023207104 (DOI: 10.3390/ani15223295, Elsevier: Scopus)
3.	T. Suong Nguyen, Takeshige Otoi, Zhao Namula, Setyo Oky Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Yuichiro Nakayama, Megumi Nagahara, Aya Nakai, Maki Hirata and Fuminori Tanihara : Mitochondrial-Targeted Protective Potential of Elamipretide for the In Vitro Production of Porcine Embryos, Animals : An Open Access Journal from MDPI, 15, 17, 2497, 2025. (キーワード) elamipretide / embryo / glutathione / ミトコンドリア (mitochondria) / porcine / 活性酸素 (reactive oxygen species) (出版サイトへのリンク) ● Publication site (DOI): 10.3390/ani15172497 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-105015468944 (DOI: 10.3390/ani15172497, Elsevier: Scopus)
4.	Bin Liu, Takeshige Otoi, Zhao Namula, Setyo Oky Widodo, Maki Hirata, Aya Nakai, Qingyi Lin, Yuichiro Nakayama, Megumi Nagahara and Fuminori Tanihara : Oxidative stress index of porcine follicular fluid influences meiotic maturation and embryo development during in vitro culture, Veterinary World, 18, 7, 2078-2086, 2025. (キーワード) embryo development / glutathione / in vitro maturation / oxidative stress index / porcine follicular fluid, reactive oxygen species (出版サイトへのリンク) ● Publication site (DOI): 10.14202/vetworld.2025.2078-2086 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-105015477974 (DOI: 10.14202/vetworld.2025.2078-2086, Elsevier: Scopus)
5.	Aya Nakai, Shiho Fukushima, Kazuaki Mawatari, Akira Takahashi, Takeshi Nikawa, Shintaro Inoue, Takahito Watanabe, Taro Mito and Katsuyuki Miyawaki : Differential Expression of Key Isoflavone Synthesis Genes in Soybean Sprouts under Two LED Treatments, ACS Agricultural Science & Technology, 2025. (要約) Soybean seeds and sprouts contain isoflavones, including aglycones, glucosides, and malonylated glucosides, and their contents vary across soybean varieties. During germination and photomorphogenesis, blue light-emitting diode (LED) irradiation suppressed hypocotyl growth and enhanced the expression levels of chalcone synthase 7, isoflavone synthase (IFS), and isoflavone reductase (IFR). Conversely, red LED irradiation enhanced hypocotyl growth and decreased the expression levels of IFS and IFR. The contents of some glycosides or malonylated glycosides in sprouts and young green soybeans were significantly higher under blue LED irradiation than under red LED irradiation. These results imply that blue light more effectively increased the concentrations of some isoflavones during early germination and/or hydroponics cultivation. (出版サイトへのリンク) ● Publication site (DOI): 10.1021/acsagscitech.4c00646 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1021/acsagscitech.4c00646 (DOI: 10.1021/acsagscitech.4c00646)
6.	Megumi Nagahara, Maki Hirata, Q Lin, Koki Takebayashi, Aya Nakai, Takeshige Otoi and Fuminori Tanihara : Analysis of myofiber composition in myostatin monoallelic mutant pigs, Journal of Livestock Science, 16, 168-172, 2025. (出版サイトへのリンク) ● Publication site (DOI): 10.33259/JLivestSci.2024.168-172 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.33259/JLivestSci.2024.168-172 (DOI: 10.33259/JLivestSci.2024.168-172)
7.	Nanaka Torigoe, Qingyi Lin, Bin Liu, Yuichiro Nakayama, Aya Nakai, Megumi Nagahara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi : Effects of Electroporation Timing and Cumulus Cell Attachment on In Vitro Development and Genome Editing of Porcine Embryos., Reproduction in Domestic Animals = Zuchthygiene, 60, 2, e70011, 2025. (要約) Pig genome editing using the oviductal nucleic acid delivery (GONAD) method with electroporation would allow the efficient obtention of genetically modified pigs. However, oocytes and zygotes at various stages after ovulation must be targeted, and cumulus cell attachment and mosaic mutations are major obstacles. Therefore, we investigated whether two parameters (electroporation timing and the cumulus cell attachment) influence the effectiveness of multiplex genome editing by electroporation in porcine oocytes or zygotes. Three gRNAs targeting either GGTA1, CMAH or B4GALNT2 were introduced individually into oocytes and zygotes with and without cumulus cells at three different time points, 0 h before in vitro fertilisation (IVF) and 5 h and 10 h after IVF initiation. The introduction of gRNAs into oocytes and zygotes did not significantly affect the rates of blastocyst formation and total mutation of the resulting blastocysts irrespective of cumulus cell attachment and electroporation timing. In conclusion, the electroporation timing and the cumulus cell attachment did not interfere with the efficient delivery of the CRISPR/Cas9 system to the oocytes/zygotes, indicating that porcine genome editing in the oviduct using GONAD method may be possible. (キーワード) Animals / Cumulus Cells / Electroporation / Gene Editing / Female / Oocytes / CRISPR-Cas Systems / Fertilization in Vitro / Embryonic Development / Swine / Zygote / RNA, Guide, CRISPR-Cas Systems / Blastocyst / Galactosyltransferases / Embryo Culture Techniques (出版サイトへのリンク) ● Publication site (DOI): 10.1111/rda.70011 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39901848 ● Search Scopus @ Elsevier (PMID): 39901848 ● Search Scopus @ Elsevier (DOI): 10.1111/rda.70011 (DOI: 10.1111/rda.70011, PubMed: 39901848)
8.	Supitcha Kaewma, Takeshige Otoi, Setyo Oky Widodo, Megumi Nagahara, Aya Nakai, T Suong Nguyen, Yuichiro Nakayama, Theerawat Tharasanit, Maki Hirata and Fuminori Tanihara : Zinc Chloride Supplementation During In Vitro Fertilization Reduces Polyspermic Fertilization of Porcine Oocytes, Animal Science Journal, 96, 1, e70095, 2025. (要約) As zinc is important for the fertilization competency of sperms and oocytes, we investigated the effects of zinc chloride (ZnCl₂) supplementation during in vitro fertilization (IVF) on porcine oocyte fertilization and development. We evaluated the effects of ZnCl₂ concentration (0, 1, 10, and 20 μg/mL) on the quality of frozen-thawed boar spermatozoa cultured for 5 h and on oocyte fertilization and embryo development after IVF. Spermatozoa from three different boars were additionally tested. ZnCl₂ supplementation effects along with metal chelators, calcium ethylenediaminetetraacetic acid (Ca-EDTA) and zinc EDTA (Zn-EDTA), were also examined. ZnCl₂ supplementation did not affect the quality of frozen-thawed spermatozoa after culture for 5 h. Supplementation with 1-μg/mL ZnCl₂ decreased the percentage of polyspermic fertilization compared to that at 0- and 20-μg/mL ZnCl₂. Moreover, it increased blastocyst formation rate compared to that in the other supplementation groups. In different boar spermatozoa, ZnCl₂ supplementation (1 μg/mL) decreased polyspermic fertilization but did not improve embryo development. Co-incubation with Ca-EDTA did not reduce polyspermic fertilization, but Zn-EDTA co-incubation reduced polyspermic fertilization similar to that with ZnCl₂ alone. In conclusion, 1-μg/mL ZnCl₂ supplementation during IVF reduces polyspermic fertilization but may not improve embryo development. (キーワード) 胚発生 (embryonal development) / in vitro fertilization / monospermy / pig / 亜鉛 (zinc) (出版サイトへのリンク) ● Publication site (DOI): 10.1111/asj.70095 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 40859724 ● Summary page in Scopus @ Elsevier: 2-s2.0-105014172522 (DOI: 10.1111/asj.70095, PubMed: 40859724, Elsevier: Scopus)
9.	Qingyi Lin, Nanaka Torigoe, Bin Liu, Yuichiro Nakayama, Aya Nakai, Zhao Namula, Megumi Nagahara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi : Efficient gene editing of pig embryos by combining electroporation and lipofection., Veterinary World, 17, 11, 2701-2707, 2024. (要約) Electroporation combined with lipofection using gRNAs with low mutation rates may improve gene editing efficiency in pig embryos. However, the effects may vary based on the timing of gene editing. (徳島大学機関リポジトリ) ● Metadata: 2013144 (出版サイトへのリンク) ● Publication site (DOI): 10.14202/vetworld.2024.2701-2707 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39829671 ● Search Scopus @ Elsevier (PMID): 39829671 ● Search Scopus @ Elsevier (DOI): 10.14202/vetworld.2024.2701-2707 (徳島大学機関リポジトリ: 2013144, DOI: 10.14202/vetworld.2024.2701-2707, PubMed: 39829671)
10.	Koki Takebayashi, Manita Wittayarat, Maki Hirata, Qingyi Lin, Zhao Namula, Nanaka Torigoe, Bin Liu, Megumi Nagahara, Aya Nakai, Takeshige Otoi and Fuminori Tanihara : Optimization of embryonic stage for aggregation to generate chimeric pigs using gene-edited blastomeres., In Vitro Cellular & Developmental Biology. Animal, 2024. (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s11626-024-00987-z (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39446279 ● Search Scopus @ Elsevier (PMID): 39446279 ● Search Scopus @ Elsevier (DOI): 10.1007/s11626-024-00987-z (DOI: 10.1007/s11626-024-00987-z, PubMed: 39446279)
11.	Aya Nakai, Akihito Tanaka, Hitoshi Yoshihara, Koji Murai, Takahito Watanabe and Katsuyuki Miyawaki : Blue LED light promotes indican accumulation and flowering in indigo plant, Polygonum tinctorium, Industrial Crops and Products, 155, 112774, 2020. (キーワード) Polygonum tinctorium / Plant factory / Blue LED / Photoperiod / Flowering / Indican (徳島大学機関リポジトリ) ● Metadata: 2007922 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.indcrop.2020.112774 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85088149064 (徳島大学機関リポジトリ: 2007922, DOI: 10.1016/j.indcrop.2020.112774, Elsevier: Scopus)

国際会議:

1.	NAKAYAMA Yuichiroh, Nguyen Thi Suong, Lin Qingyi, MATSUSHITA Mao, Aya Nakai, Maki Hirata, Megumi Nagahara, Takeshige Otoi and Fuminori Tanihara : Trials to identify factors associated with reduced embryo viability following multiplex gene editing in pigs, 20th International congress on animal reproduction, Jun. 2026.
2.	Nguyen Thi Suong, Batjargai Knishigjargal, Kashiwakura Yuji, NAKAYAMA Yuichiroh, Megumi Nagahara, Aya Nakai, Maki Hirata, Ohmori Tsukasa and Fuminori Tanihara : Adeno-associated virus vector-mediated delivery of base editors into porcine zygotes to induce a pathology-related point mutation in the Ornithine Transcarbamylase gene, 20th International congress on animal reproduction, Jun. 2026.
3.	MATSUSHITA Mao, NAKAYAMA Yuichiroh, TORIGOE Nanaka, Nguyen Thi Suong, Aya Nakai, Maki Hirata, Fuminori Tanihara, Takeshige Otoi and Megumi Nagahara : Continuous exposure to 228-nm UVC could serve as a safe and practical approach for maintaining a hygienic rearing environment in piglet production, 20th International congress on animal reproduction, Jun. 2026.

国内講演発表:

1.	長原恵, 中山雄一郎, Nguyen Thi Suong, 平田真樹, 中井綾, 柏倉裕志, 大森司, 谷原史倫 : 腹腔鏡システムを用いた体外受精卵の胚移植によるゲノム編集ブタの作出, 第168回日本獣医学会学術集会, 2025年9月.

その他・研究会:

1.	中井綾, 村井恒治, 田中昭人, 近藤宏, 渡邊杉菜, 上番増明子, 渡辺崇人, 宮脇克行 : LED植物工場を活用した藍の生育と二次代謝物質生産の制御, LED総合フォーラム2019 in徳島, 63-64, 2019年2月.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

地球温暖化対策に向けたブタ暑熱耐性遺伝子の選出及び機能解析 (研究課題/領域番号: 25K09406 )
研究者番号（70829019）による検索