Tokushima University / Educator and Researcher Directory --- Mineda, Ayuka

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Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 医学域 ⟩ 先端医学教育研究プロジェクト ⟩
Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 医学域 ⟩ Medical Science ⟩ Surgery ⟩ Department of Obstetrics and Gynecology ⟩
Tokushima University ⟩ University Hospital ⟩ Clinical Division ⟩ Department of Pediatrics,Obstetrics and Gynecology ⟩ Obstetrics snd Gynecology ⟩

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Academic Paper (Judged Full Paper):

1.	Akiko Abe, Masao Yuasa, Yoshie Imai, Tomohiro Kagawa, Ayuka Mineda, Masato Nishimura, Chisato Tonoiso, Akiko Kubo, Takashi Kawanaka, Hitoshi Ikushima and Takeshi Iwasa : Extreme leanness, lower skeletal muscle quality, and loss of muscle mass during treatment are predictors of poor prognosis in cervical cancer treated with concurrent chemoradiation therapy, International Journal of Clinical Oncology, Vol.27, No.5, 983-991, 2022. (Summary) Human papillomavirus vaccination is not widespread in Japan, and the low screening rates result in many cases of locally advanced cervical cancer. We investigated the prognostic significance of sarcopenia in patients with cervical cancer to guide healthcare policies to improve treatment outcomes. This retrospective study included 83 patients with cervical cancer without distant metastasis who underwent primary concurrent chemoradiotherapy between 2013 and 2018. We analyzed the indicators of physical condition and muscle quantity using the SYNAPSE VINCENT software. Muscle mass and the relationship between treatment toxicity and prognosis were evaluated. The patients' median age was 60 (range 33-80) years. Cancer stage distribution was as follows: cT2b or higher, 84.3%; N1, 65.1%; and MA, 27.7%. The overall sarcopenia (skeletal muscle index [SMI] < 38.5) rate was 30.1%, and the rate was 33.9 and 22.2% in patients aged < 64 and ≥ 65 years, respectively. No correlation was observed between clinical stage and musculoskeletal indices. Treatment resulted in decreased body weight and SMI; after treatment, the sarcopenia rate increased to 37.3%. A higher intramuscular adipose tissue content (IMAC) reduced the number of chemotherapy cycles needed. Treatment-associated SMI decreases of ≥ 7% indicated poor prognosis, with significant differences in progression-free survival and overall survival (p = 0.013 and p = 0.012, respectively). Patients who were very lean (body mass index < 18.5 kg/m) before treatment had a poor prognosis (p = 0.016 and p < 0.001). Our findings emphasize the importance of assessing original nutritional status and maintaining muscle mass and quality during the treatment of patients with cervical cancer. (Keyword) Adult / Aged / Aged, 80 and over / Chemoradiotherapy / Female / Humans / Middle Aged / Muscle, Skeletal / Papillomavirus Infections / Papillomavirus Vaccines / Prognosis / Retrospective Studies / Sarcopenia / Thinness / Uterine Cervical Neoplasms (Link to Publication Site) ● Publication site (DOI): 10.1007/s10147-022-02140-w (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35212828 ● Summary page in Scopus @ Elsevier: 2-s2.0-85125283470 (DOI: 10.1007/s10147-022-02140-w, PubMed: 35212828, Elsevier: Scopus)
2.	Ayuka Mineda, Masato Nishimura, Tomohiro Kagawa, Eri Takiguchi, Takako Kawakita, Akiko Abe and Minoru Irahara : Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway., Experimental and Therapeutic Medicine, Vol.17, No.3, 2242-2246, 2019. (Summary) Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma. (Tokushima University Institutional Repository) ● Metadata: 113338 (Link to Publication Site) ● Publication site (DOI): 10.3892/etm.2019.7209 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30867708 ● Search Scopus @ Elsevier (PMID): 30867708 ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2019.7209 (Tokushima University Institutional Repository: 113338, DOI: 10.3892/etm.2019.7209, PubMed: 30867708)
3.	Masaharu Kamada, Hiroaki Inui, Tomohiro Kagawa, Ayuka Mineda, Takao Tamura, Tomohito Fujioka, Takahiro Motoki, Hiroki Hirai, Eiichi Ishii and Minoru Irahara : What information can change the attitude of teachers toward the human papillomavirus vaccine?, The Journal of Obstetrics and Gynaecology Research, Vol.44, No.4, 778-787, 2018. (Summary) We conducted a self-administered survey on the perception of teachers toward human papillomavirus (HPV) vaccine to determine the ways to increase their willingness to encourage its use. Answers were obtained both prior to and after having the teachers read five brief information articles: (i) cervical cancer knowledge, (ii) vaccine knowledge, (iii) result of a survey in Nagoya, (iv) news report of the World Health Organization statement and (v) articles written by Dr Muranaka, a journalist. Most of the respondents (180/247) did not know about the natural history of cervical cancer. Only 36% knew that HPV is the cause of cervical cancer, although 63% knew that HPV vaccine would prevent cervical cancer. Few respondents had knowledge regarding adverse events following immunization and the survey results from Nagoya. Among those who were initially negative for the HPV vaccine, only 43% revealed that they fully understood its safety and only 29% reversed their opinion to recommend vaccination to their daughters and/or students, even after reading our informational material. The most useful information for changing their attitudes was to increase their understanding of vaccines and informing them about Nagoya city survey results. They mostly wanted a proof of the preventive effects of the vaccine on cervical cancer in Japan. Gynecologists and pediatricians must proactively communicate accurate scientific information to the government and the media to spread awareness among people in Japan. Also, we must try to demonstrate the capabilities of this vaccine to prevent cervical cancer and/or its precancerous lesions. (Link to Publication Site) ● Publication site (DOI): 10.1111/jog.13584 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29442400 ● Search Scopus @ Elsevier (PMID): 29442400 ● Search Scopus @ Elsevier (DOI): 10.1111/jog.13584 (DOI: 10.1111/jog.13584, PubMed: 29442400)
4.	Eri Takiguchi, Masato Nishimura, Ayuka Mineda, Takako Kawakita, Akiko Abe and Minoru Irahara : Growth inhibitory effect of the Src inhibitor dasatinib in combination with anticancer agents on uterine cervical adenocarcinoma cells., Experimental and Therapeutic Medicine, Vol.14, No.5, 4293-4299, 2017. (Summary) Uterine cervical adenocarcinoma has a poor clinical prognosis when compared with squamous cell carcinoma. Therefore, the development of new treatment strategies for uterine cervical adenocarcinoma is necessary. Src is a proto-oncogene that is important in cancer progression. Dasatinib is a Src inhibitor that has been reported to be effective when used in combination with anticancer drugs. The present study aimed to confirm Src expression in human cervical adenocarcinoma cell lines and to determine the mechanism underlying the inhibitory effect of dasatinib on Src signaling . Western blot analysis was performed to investigate Src expression in cervical adenocarcinoma cell lines (HeLa and TCO-2 cells). The cells were cultured for 48 h with the addition of different concentrations of anticancer drugs (paclitaxel or oxaliplatin). Viable cell count was measured using a colorimetric (WST-1) assay. The concentrations of anticancer agents were fixed according to the results obtained, and the same experiments were performed using the drugs in combination with dasatinib at various concentrations to determine the concentrations that significantly affected the number of viable cells. The presence or absence of apoptosis was investigated using a caspase-3/7 assay. Signal transduction in each cell line was examined using western blotting. Src was activated in the two cell lines, and cell proliferation was significantly suppressed by each anticancer drug in combination with 10 µM dasatinib. Caspase-3/7 activity was also increased and Src signaling was suppressed by each anticancer drug in combination with dasatinib. In conclusion, Src is overexpressed in cervical adenocarcinoma cell lines, and dasatinib inhibits intracellular Src signaling and causes apoptosis. The results of the present study suggest that Src may be targeted in novel therapeutic strategies for cervical adenocarcinoma. (Tokushima University Institutional Repository) ● Metadata: 112973 (Link to Publication Site) ● Publication site (DOI): 10.3892/etm.2017.5061 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29067110 ● Search Scopus @ Elsevier (PMID): 29067110 ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2017.5061 (Tokushima University Institutional Repository: 112973, DOI: 10.3892/etm.2017.5061, PubMed: 29067110)

Academic Paper (Unrefereed Paper):

1.	Takeshi Iwasa, Hiroki Noguchi, Risa Tanano, Erika Yamanaka, Asuka Takeda, Kou Tamura, Hidenori Aoki, Tatsuro Sugimoto, Hikari Sasada, Takaaki Maeda, Saki Minato, Shota Yamamoto, Hiroaki Inui, Tomohiro Kagawa, Atsuko Yoshida, Ayuka Mineda, Mari Nii, Riyo Kinouchi, Kanako Yoshida, Yuri Yamamoto and Takashi Kaji : Age-Dependent Changes in the Effects of Androgens on Female Metabolic and Body Weight Regulation Systems in Humans and Laboratory Animals., International Journal of Molecular Sciences, Vol.24, No.23, 16567, 2023. (Summary) In recent years, the effects of androgens on metabolic and body weight regulation systems and their underlying mechanisms have been gradually revealed in females. In women and experimental animals of reproductive age, androgen excess can adversely affect metabolic functioning, appetite, and body weight regulation. In addition, excess androgens can increase the risk of metabolic disorders, such as obesity, insulin resistance, and diabetes. These unfavorable effects of androgens are induced by alterations in the actions of hypothalamic appetite-regulatory factors, reductions in energy expenditure, insulin resistance in skeletal muscle, and β-cell dysfunction. Interestingly, these unfavorable effects of androgens on metabolic and body-weight regulation systems are neither observed nor evident in ovariectomized animals and post-menopausal women, indicating that the adverse effects of androgens might be dependent on the estrogen milieu. Recent findings may provide novel sex- and age-specific strategies for treating metabolic diseases. (Keyword) Animals / Humans / female / Androgens / Insulin Resistance / Obesity / Metabolic Diseases / Animals, Laboratory / Polycystic Ovary Syndrome (Link to Publication Site) ● Publication site (DOI): 10.3390/ijms242316567 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38068890 ● Search Scopus @ Elsevier (PMID): 38068890 ● Search Scopus @ Elsevier (DOI): 10.3390/ijms242316567 (DOI: 10.3390/ijms242316567, PubMed: 38068890)
2.	Naoto Yonetani, Takashi Kaji, Aya Shirakawa, Eishi Sogawa, Atsuko Yoshida, Ayuka Mineda, Kazuhisa Maeda, Minoru Irahara and Takeshi Iwasa : Sonographic confirmation of placental detachment during placental delivery for uterine inversion prevention., The Journal of Obstetrics and Gynaecology Research, Vol.49, No.11, 2644-2648, 2023. (Summary) Transabdominal sonographic confirmation of placental detachment may be useful in preventing uterine inversion. (Keyword) Pregnancy / Female / Humans / Placenta / Uterine Inversion / Retrospective Studies / Ultrasonography / Delivery, Obstetric / Obstetric Labor Complications (Link to Publication Site) ● Publication site (DOI): 10.1111/jog.15763 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37551066 ● Search Scopus @ Elsevier (PMID): 37551066 ● Search Scopus @ Elsevier (DOI): 10.1111/jog.15763 (DOI: 10.1111/jog.15763, PubMed: 37551066)
3.	Junki Imaizumi, Shuhei Kamada, Miyu Taniguchi, Tatsuro Sugimoto, Takaaki Maeda, Ryosuke Arakaki, Shota Yamamoto, Aya Shirakawa, Ayuka Mineda, Atsuko Yoshida, Takeshi Iwasa and Takashi Kaji : Developmental Changes in Hypothalamic and Serum Oxytocin Levels in Prenatally Normally Nourished and Undernourished Rats., Nutrients, Vol.15, No.12, 2768, 2023. (Summary) Changes in the activities of some metabolic factors have been suggested to increase the risk of conditions associated with the Developmental Origins of Health and Disease (DOHaD). We examined changes in oxytocin (OT), a metabolic factor, and OT receptor (OTR) mRNA levels throughout the developmental period in rats of intrauterine undernutrition. Pregnant rats were divided into two groups: a maternal normal nutrition (mNN) and maternal undernutrition (mUN) group. Serum OT concentrations and hypothalamic mRNA levels of OT and OTR were measured in both offspring at various postnatal stages. Both offspring showed significant increases in serum OT concentrations during the neonatal period, significant reductions around the pubertal period, and significant increases in adulthood. Hypothalamic OT mRNA expression levels gradually increased from the neonatal to pubertal period and decreased in adulthood in both offspring. In the pre-weaning period, hypothalamic OT mRNA expression levels were significantly lower in the mUN offspring than in the mNN offspring. In the mUN offspring, hypothalamic OTR mRNA expression levels transiently increased during the neonatal period, decreased around the pubertal period, and increased again in adulthood, whereas transient changes were not detected in mNN offspring. These changes could affect nutritional and metabolic regulation systems in later life and play a role in the mechanisms underlying DOHaD. (Keyword) Pregnancy / Female / Rats / Animals / Oxytocin / Rats, Sprague-Dawley / Malnutrition / Weaning / RNA, Messenger (Link to Publication Site) ● Publication site (DOI): 10.3390/nu15122768 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37375670 ● Search Scopus @ Elsevier (PMID): 37375670 ● Search Scopus @ Elsevier (DOI): 10.3390/nu15122768 (DOI: 10.3390/nu15122768, PubMed: 37375670)
4.	篠原文香, Ayuka Mineda, 乾宏彰, Tomohiro Kagawa, 阿部彰子, 西條康代, Masato Nishimura, Minoru Irahara and Takeshi Iwasa : 術前に卵巣原発胚細胞性腫瘍を疑ったが, 傍卵巣の平滑筋肉腫であった一例, Modern Trends in Obstetrics & Gynecology, Vol.70, No.1, 227-231, 2021. (Keyword) 骨盤内腫瘍 / 平滑筋肉腫 / 画像評価 / 栄養血管 / pelvic tumor / leiomyosarcoma / image evaluation / feeding blood vessels / コツバンナイシュヨウ / ヘイカツキンニクシュ / ガゾウヒョウカ / エイヨウケッカン (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520290885604307712 (CiNii: 1520290885604307712)
5.	Akiko Abe, Tomohiro Kagawa, 祖川英至, Ayuka Mineda and Masato Nishimura : Mismatch repair deficiency in young patients of reproductive age with endometrial cancer and atypical endometrial hyperplasia, Journal of Hereditary Tumors, Vol.20, No.3, 146-150, 2020. (Summary) <p>MPA therapy is widely administered to patients with early-stage endometrial cancer and atypical endometrial hyperplasia (AEH) who desire a fertility-preserving treatment modality. We examined the relationship between MMR protein expression and the effects of MPA therapy, risk of relapse, prognosis, and familial history in 21 patients. We observed MMR deficiency in 7 patients (MLH1: 4, PMS2: 5, MSH2: 2, and MSH6: 2). All patients with lymph node metastasis or who died had MLH1 deficiency. In 13 of 21 patients, we performed MPA therapy for fertility preservation and confirmed MMR deficiency in 2. After the initial treatment, ovarian cancer developed in 2 patients, one of whom had Peutz-Jeghers syndrome. Due to the small sample size of this study, we found no significant association between MMR deficiency and the effects of MPA therapy or the risk of relapse. Many young patients with endometrial tumors may have inherited diseases, although not necessarily with MMR deficiency. Therefore, clinical assessment considering their past history and family history should be performed prior to each treatment. Our findings will serve as a catalyst for future studies with larger sample sizes.</p> (Keyword) endometrial cancer / mismatch repair deficiency / hereditary tumor (Link to Publication Site) ● Publication site (DOI): 10.18976/jsht.20.3_146 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390286981364673792 ● Search Scopus @ Elsevier (DOI): 10.18976/jsht.20.3_146 (DOI: 10.18976/jsht.20.3_146, CiNii: 1390286981364673792)
6.	Tomohiro Kagawa, Akiko Abe, Hiroaki Inui, Ayuka Mineda and Masato Nishimura : 嚢胞状帝王切開瘢痕部が着床部結節から成っていた一例, Modern Trends in Obstetrics & Gynecology, Vol.69, No.1, 5-9, 2020. (Keyword) cesarean scar syndrome / intermediate trophoblast / placental site nodule (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520572359820341376 (CiNii: 1520572359820341376)

Proceeding of International Conference:

1.	Akiko Abe, Akira Kuwahara, Tomohiro Kagawa, Ayuka Mineda and Masato Nishimura : A survey of germline mutations with epithelial ovarian cancer in Japanese patients., ESGO Annual Meeting 2019, Greece, Nov. 2019.

Proceeding of Domestic Conference:

1.	Takashi Kaji, Tomotaka Nakagawa, Atsuko Yoshida, Aya Shirakawa, Ayuka Mineda, Mikio Sugano, Yasunobu Hayabuchi and Takeshi Iwasa : ポスタ-発表胎児2 手術を要した単独VSDの胎児診断および出生後診断についての検討, 第59回日本小児循環器学会総会·学術集会, Jul. 2023.
2.	Kanako Yoshida, Takeshi Katou, 武田明日香, Ryosuke Arakaki, 今泉絢貴, Tomohiro Kagawa, Ayuka Mineda, 河北貴子, Yuri Yamamoto, 苛原稔 and Takeshi Iwasa : ワークショップ:効率よくTLH上達を目指すための当科での取り組み, 第62回日本産婦人科内視鏡学会, Sep. 2022.
3.	Kanako Yoshida, Yuri Kadota, Kana Kasai, Ayuka Mineda, Takako Kawakita, Takeshi Katou, Masato Nishimura, Minoru Irahara and Takeshi Iwasa : 腹腔鏡下仙骨腟固定術(LSC)における後腹膜閉鎖の工夫, 第60回日本産婦人科内視鏡学会学術講演会, Dec. 2020.
4.	Takeshi Iwasa, Toshiya Matsuzaki, Akiko Abe, 柳原理江, Yuri Yamamoto, Tomohiro Kagawa, Ayuka Mineda, Masato Nishimura, Akira Kuwahara and Minoru Irahara : 子宮内膜異型増殖症・初期子宮体癌患者に対する黄体ホルモン療法の治療成績，妊娠成績および周産期予後, 第72回中国四国日本産科婦人科学会学術講演会, Sep. 2019.
5.	Tomohiro Kagawa, Akiko Abe, Ayuka Mineda and Masato Nishimura : 嚢胞状帝王切開瘢痕部が着床部結節からなっていた一例, 第72回中国四国日本産科婦人科学会学術講演会, Sep. 2019.
6.	Akiko Abe, Issei Imoto, Tomohiro Kagawa, Ayuka Mineda, Masato Nishimura and Minoru Irahara : 上皮性卵巣癌に対する遺伝学的検査への意識調査とHRD遺伝子変異の検討, 第61回日本婦人科腫瘍学会, Jul. 2019.
7.	Masato Nishimura, Ayuka Mineda, Akiko Abe, Kanako Yoshida, Takeshi Katou and Minoru Irahara : 若年におけるHPVワクチン接種は子宮頸癌検診における細胞診異常の頻度を低下させる, 第60回日本臨床細胞学会学術講演会, Jun. 2019.

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

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