Tokushima University / Educator and Researcher Directory --- Kondo, Tomoyuki

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Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 医学域 ⟩ 先端基礎医学教育研究プロジェクト ⟩
Tokushima University ⟩ Graduate School of Biomedical Sciences ⟩ 医学域 ⟩ Medical Science ⟩ Pathological Sciences ⟩ Department of Microbiology ⟩
Tokushima University ⟩ Faculty of Medicine ⟩ School of Medicine ⟩ Course of Microbiology and Immunology ⟩ Microbiology ⟩

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Research

Field of Study

Subject of Study

Book / Paper

Book:

1.	Takaaki Koma, Naoya Doi, Le Quoc Bao, Tomoyuki Kondo, Akio Adachi and Masako Nomaguchi : HIV-1 Replication and Pathogenicity: Lessons from Macaque-Tropic HIV-1 Derivatives, IntechOpen, London, Sep. 2023. (Link to Publication Site) ● Publication site (DOI): 10.5772/intechopen.1002899 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.5772/intechopen.1002899 (DOI: 10.5772/intechopen.1002899)

Academic Paper (Judged Full Paper):

1.	Takaaki Koma, Naoya Doi, Bao Quoc Le, Tomoyuki Kondo, Mitsuki Ishizue, Chiaki Tokaji, Chizuko Tsukada, Akio Adachi and Masako Nomaguchi : Involvement of a Rarely Used Splicing SD2b Site in the Regulation of HIV-1 mRNA Production as Revealed by a Growth-Adaptive Mutation., Viruses, Vol.15, No.12, 2424, 2023. (Summary) We have previously reported an HIV-1 mutant designated NL-Y226tac that expresses Vif at an ultra-low level, being replication-defective in high-APOBEC3G cells, such as H9. It carries a synonymous mutation within the splicing SA1 site relative to its parental clone. In order to determine whether a certain mutant(s) emerges during multi-infection cycles, we maintained H9 cells infected with a relatively low or high input of NL-Y226tac for extended time periods. Unexpectedly, we reproducibly identified a g5061a mutation in the SD2b site in the two independent long-term culture experiments that partially increases Vif expression and replication ability. Importantly, the adaptive mutation g5061a was demonstrated to enhance mRNA production by activation of the SA1 site mediated through increasing usage of a rarely used SD2b site. In the long-term culture initiated by a high virus input, we additionally found a Y226Fttc mutation at the original Y226tac site in SA1 that fully restores Vif expression and replication ability. As expected, the adaptive mutation Y226Fttc enhances mRNA production through increasing the splicing site usage of SA1. Our results here revealed the importance of the SD2b nucleotide sequence in producing mRNA involved in the HIV-1 adaptation and of mutual antagonism between Vif and APOBEC3 proteins in HIV-1 adaptation/evolution and survival. (Keyword) RNA, Messenger / HIV-1 / Cell Line / vif Gene Products, Human Immunodeficiency Virus / Virus Replication / Cytidine Deaminase / Mutation (Link to Publication Site) ● Publication site (DOI): 10.3390/v15122424 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38140666 ● Summary page in Scopus @ Elsevier: 2-s2.0-85180515625 (DOI: 10.3390/v15122424, PubMed: 38140666, Elsevier: Scopus)
2.	Takaaki Koma, Tokifumi Odaka, Sung-il Lee, Naoya Doi, Tomoyuki Kondo, Kazu Okuma, Jun-Ichi Fujisawa, Akio Adachi and Masako Nomaguchi : Humanized mice generated by intra-bone marrow injection of CD133-positive hematopoietic stem cells: application to HIV-1 research, Frontiers in Virology, Vol.3, 2023. (Link to Publication Site) ● Publication site (DOI): 10.3389/fviro.2023.1192184 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3389/fviro.2023.1192184 (DOI: 10.3389/fviro.2023.1192184)

Proceeding of International Conference:

1.	Noriaki Minakawa, Noriko Saito-Tarashima, Takaaki Koma, NAOTO Hinotani, YOSHIDA Keigo, OGASA Moka, AKIHO Murai, INOUE Shuya, Tomoyuki Kondo, Naoya Doi, Koichi Tsuneyama and Masako Nomaguchi : 3-Deazaguanosine exhibits anti-SARS-CoV-2 activity and blocks the development of COVID-19 pneumonis in hamsters., Supra FIBER International Summit for Nucleic Acids (S-FISNA) 2024, Mar. 2024.

Proceeding of Domestic Conference:

1.	Tomoyuki Kondo, Takaaki Koma, Naoya Doi, (名) bao, (名) khanh, ASAI Daiki, UTSUMI Aoi, JIYO-NZU Maritsusa Sayako, TOKUTSU Seijiro, MAEHARA Ayaka, Akio Adachi and Masako Nomaguchi : SA3周辺の塩基配列がHIV-1の複製能に与える影響の解析, 第38回中国四国ウイルス研究会, Jul. 2024.
2.	Bao Quoc Le, Yokoyama Masaru, Naoya Doi, ICHINOMIYA Takumi, KOMODA Nanako, Tomoyuki Kondo, Akio Adachi, Kotani Osamu, Sato Hironori, Masako Nomaguchi and Takaaki Koma : The importance of a novel ITI triplet motif within Env V3 domain for R5-tropic HIV-1 replication, 第70回日本ウイルス学会学術集会, Sep. 2023.
3.	Takaaki Koma, RE Kuokku Bao, Naoya Doi, KOMODA Nanako, ICHINOMIYA Takumi, Tomoyuki Kondo, Akio Adachi and Masako Nomaguchi : Implications of Gag-NC and gRNA interactions in HIV-1 assembly, 第70回日本ウイルス学会学術集会, Sep. 2023.
4.	Tomoyuki Kondo, Takaaki Koma, Naoya Doi, RE Kuokku Bao, KOMODA Nanako, ICHINOMIYA Takumi, Akio Adachi and Masako Nomaguchi : Effect of naturally-occurring synonymous single-nucleotide mutations within the vpr-coding sequence on HIV-1 replication, 第70回日本ウイルス学会学術集会, Sep. 2023.
5.	Naoya Doi, Takaaki Koma, LE Quoc Bao, KOMODA Nanako, ICHINOMIYA Takumi, Tomoyuki Kondo, Akio Adachi and Masako Nomaguchi : PIM kinases suppress HIV virion production by inhibiting viral protein expression, 第70回日本ウイルス学会学術集会, Sep. 2023.
6.	Takaaki Koma, 小谷治, Naoya Doi, Tomoyuki Kondo, 横山勝, Akio Adachi, 佐藤裕徳 and Masako Nomaguchi : HIV-1 Gag MAのGag二量体化における役割の解明, 第36回日本エイズ学会, Nov. 2022.
7.	Tomoyuki Kondo, Takaaki Koma, Akio Adachi, Masako Nomaguchi and Naoya Doi : PIMキナーゼによるHIV型特異的な遺伝子発現調節の解析, 第36回日本エイズ学会学術集会・総会, Nov. 2022.
8.	Naoya Doi, Takaaki Koma, Chisato Gotohda, NAGASAKA Mari, Tomoyuki Kondo, Akio Adachi and Masako Nomaguchi : Importance of vpr-coding sequence in HIV-1 gene expression, 第69回日本ウイルス学会学術集会, Nov. 2022.
9.	Tomoyuki Kondo, Takaaki Koma, UDAGAWA Mei, OKUMURA Nozomi, Akio Adachi, Masako Nomaguchi and Naoya Doi : Analysis of HIV type-specific regulation of viral gene expression by PIM, 第69回日本ウイルス学会学術集会, Nov. 2022.
10.	Takaaki Koma, 小谷治, Naoya Doi, Tomoyuki Kondo, 横山勝, Akio Adachi, 佐藤裕徳 and Masako Nomaguchi : HIV-1 Gag-MAにおけるGag前駆体二量体化部位のウイルス学的解析, 第69回日本ウイルス学会, Nov. 2022.
11.	Tomoyuki Kondo, Takaaki Koma, Akio Adachi, Masako Nomaguchi and Naoya Doi : PIMキナーゼ及びPIM阻害剤によるHIV種特異的な遺伝子発現と複製への影響, 第265回徳島医学会学術集会, Jul. 2022.
12.	Takaaki Koma, Naoya Doi, Tomoyuki Kondo and Masako Nomaguchi : HIV-1 matrixタンパク質のウイルス粒子集合初期過程における役割解明, 第266回徳島医学学術集会, 2022.

Patent:

1.	Masako Nomaguchi, Takaaki Koma, Naoya Doi, Tomoyuki Kondo, YOKOYAMA Masaru, SATO Hironori and Kotani Osamu : ウイルスタンパク質のセクター同定方法及び同定されたセクターの利用, (Jun. 2024), 2024-094047 (Jun. 2024).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Envの動的性質阻害変異を用いたHIV広域中和抗体誘導能に関する実証研究 (Project/Area Number: 23K07940 )
Analysis of tumor immunity in autoimmune conditions promoting tumorigenesis (Project/Area Number: 20K18484 )
Clarification of tumor immune system in chemical carcinogenic autoimmune model mice (Project/Area Number: 17K17964 )
Search by Researcher Number (10782873)