Tokushima University / Educator and Researcher Directory --- Miyawaki, Katsuyuki

Field of Study

○	Molecular Biology

Subject of Study

○	development and evolution in insects (RNA interference, segmentation, cricket)

Book / Paper

Book:

1.	Masaki Hashizume, Koichi Ute, Yumiko Izaki, Masaki Oono, Susumu Nakano, Akira Mizobuchi, Junji Tamatani, Naoyuki Shimomura, Takao Ueda, Katsutoshi Ueno, Fumiaki Nagao, Chikanori Hashimoto, Yasunori Muto, Takeshi Watanabe, Takahiro Kido, Tetsuro Katayama, Yoko Yamashita, Atsushi Tabata, Yuka Sasaki, 島木美香, Takeshige Otoi, Kaori Kanemaru, Takefumi Hattori, Tatsuo Hamano, Katsuyuki Miyawaki, Ryo Anma, Ken-ichi Nishiyama and Mutsuki Aoya : 安全マニュアル, Apr. 2021.
2.	Katsuyuki Miyawaki, Mutsumi Aihara, Akira Takahashi and Takeshi Nikawa : LEDを用いた近未来宇宙植物工場の開発, Technical Information Institute Co., Ltd., Apr. 2020.
3.	Katsuyuki Miyawaki, Sumihare Noji and Noriho Kamiya : Transglutaminase-mediated in situ hybridization (TransISH) for mRNA detection in mammalian tissues, Jan. 2015.
4.	Taro Mito, Yoshiko Inoue, S Kimura, Katsuyuki Miyawaki, Nao Niwa, Yohei Sinmyo, Hideyo Ohuchi and Sumihare Noji : Formation of new organizing regions by cooperation of hedgehog, wingless, and dpp in regeneration of the insect leg; a verification of the boundary model In Morphogenesis and pattern formation in biological systems (Sekimura et al. eds.), Springer, 2003.

Academic Paper (Judged Full Paper):

1.	Shintaro Inoue, Takahito Watanabe, Taiki Hamaguchi, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito : Combinatorial expression of ebony and tan generates body color variation from nymph through adult stages in the cricket, Gryllus bimaculatus., PLoS ONE, Vol.18, No.5, 2023. (Summary) Insect body colors and patterns change markedly during development in some species as they adapt to their surroundings. The contribution of melanin and sclerotin pigments, both of which are synthesized from dopamine, to cuticle tanning has been well studied. Nevertheless, little is known about how insects alter their body color patterns. To investigate this mechanism, the cricket Gryllus bimaculatus, whose body color patterns change during postembryonic development, was used as a model in this study. We focused on the ebony and tan genes, which encode enzymes that catalyze the synthesis and degradation, respectively, of the precursor of yellow sclerotin N-β-alanyl dopamine (NBAD). Expression of the G. bimaculatus (Gb) ebony and tan transcripts tended to be elevated just after hatching and the molting period. We found that dynamic alterations in the combined expression levels of Gb'ebony and Gb'tan correlated with the body color transition from the nymphal stages to the adult. The body color of Gb'ebony knockout mutants generated by CRISPR/Cas9 systemically darkened. Meanwhile, Gb'tan knockout mutants displayed a yellow color in certain areas and stages. The phenotypes of the Gb'ebony and Gb'tan mutants probably result from an over-production of melanin and yellow sclerotin NBAD, respectively. Overall, stage-specific body color patterns in the postembryonic stages of the cricket are governed by the combinatorial expression of Gb'ebony and Gb'tan. Our findings provide insights into the mechanism by which insects evolve adaptive body coloration at each developmental stage. (Keyword) Animals / Melanins / Gryllidae / Nymph / Dopamine / Insect Proteins (Link to Publication Site) ● Publication site (DOI): 10.1371/journal.pone.0285934 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37200362 ● Search Scopus @ Elsevier (PMID): 37200362 ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0285934 (DOI: 10.1371/journal.pone.0285934, PubMed: 37200362)
2.	Aya Nakai, Akihito Tanaka, Hitoshi Yoshihara, Koji Murai, Takahito Watanabe and Katsuyuki Miyawaki : Blue LED light promotes indican accumulation and flowering in indigo plant, Polygonum tinctorium, Industrial Crops and Products, Vol.155, 112774, 2020. (Keyword) Polygonum tinctorium / Plant factory / Blue LED / Photoperiod / Flowering / Indican (Tokushima University Institutional Repository) ● Metadata: 114990 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.indcrop.2020.112774 (Link to Search Site for Scientific Articles) ● Summary page in Scopus @ Elsevier: 2-s2.0-85088149064 (Tokushima University Institutional Repository: 114990, DOI: 10.1016/j.indcrop.2020.112774, Elsevier: Scopus)
3.	Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji and Taro Mito : TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis., Proceedings of the National Academy of Sciences of the United States of America, Vol.113, No.20, 5634-5639, 2016. (Summary) Although butterflies undergo a dramatic morphological transformation from larva to adult via a pupal stage (holometamorphosis), crickets undergo a metamorphosis from nymph to adult without formation of a pupa (hemimetamorphosis). Despite these differences, both processes are regulated by common mechanisms that involve 20-hydroxyecdysone (20E) and juvenile hormone (JH). JH regulates many aspects of insect physiology, such as development, reproduction, diapause, and metamorphosis. Consequently, strict regulation of JH levels is crucial throughout an insect's life cycle. However, it remains unclear how JH synthesis is regulated. Here, we report that in the corpora allata of the cricket, Gryllus bimaculatus, Myoglianin (Gb'Myo), a homolog of Drosophila Myoglianin/vertebrate GDF8/11, is involved in the down-regulation of JH production by suppressing the expression of a gene encoding JH acid O-methyltransferase, Gb'jhamt In contrast, JH production is up-regulated by Decapentaplegic (Gb'Dpp) and Glass-bottom boat/60A (Gb'Gbb) signaling that occurs as part of the transcriptional activation of Gb'jhamt Gb'Myo defines the nature of each developmental transition by regulating JH titer and the interactions between JH and 20E. When Gb'myo expression is suppressed, the activation of Gb'jhamt expression and secretion of 20E induce molting, thereby leading to the next instar before the last nymphal instar. Conversely, high Gb'myo expression induces metamorphosis during the last nymphal instar through the cessation of JH synthesis. Gb'myo also regulates final insect size. Because Myo/GDF8/11 and Dpp/bone morphogenetic protein (BMP)2/4-Gbb/BMP5-8 are conserved in both invertebrates and vertebrates, the present findings provide common regulatory mechanisms for endocrine control of animal development. (Tokushima University Institutional Repository) ● Metadata: 116415 (Link to Publication Site) ● Publication site (DOI): 10.1073/pnas.1600612113 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27140602 ● Summary page in Scopus @ Elsevier: 2-s2.0-84969765307 (Tokushima University Institutional Repository: 116415, DOI: 10.1073/pnas.1600612113, PubMed: 27140602, Elsevier: Scopus)
4.	Y Kadomura-Ishikawa, Katsuyuki Miyawaki, Akira Takahashi, Toshiya Masuda and Sumihare Noji : Light and abscisic acid independently regulated FaMYB10 in Fragaria x ananassa fruit, Planta, Vol.241, No.4, 953-965, 2015. (Summary) Light and ABA independently regulated anthocyanin biosynthesis via activation of FaMYB10 expression. FaMYB10 accelerated anthocyanin synthesis of pelargonidin 3-glucoside and cyanidin 3-glucoside during strawberry fruit ripening. Light is an integral factor in fruit ripening. Ripening in non-climacteric fruit is also effected by the plant hormone abscisic acid (ABA). However, how light and/or ABA regulate fruit ripening processes, such as strawberry color development remains elusive. Results of the present study showed light and ABA regulated strawberry fruit coloration via activation of FaMYB10 expression, an R2R3 MYB transcription factor. Light exposure increased FaMYB10 transcript levels, flavonoid pathway genes, and anthocyanin content. Exogenous ABA promoted FaMYB10 expression, and anthocyanin content, accompanied by increased ABA-responsive transcript levels and flavonoid pathway genes. ABA biosynthesis inhibitor treatment, and RNAi-mediated down-regulation of the ABA biosynthetic gene (9-cis epoxycarotenoid dioxygenase: FaNCED1), and ABA receptor (magnesium chelatase H subunit: FaCHLH/ABAR) showed inverse ABA effects. Furthermore, additive effects were observed in anthocyanin accumulation under combined light and ABA, indicating independent light and ABA signaling pathways. FaMYB10 down-regulation by Agrobacterium-mediated RNA interference (RNAi) in strawberry fruits showed decreased pelargonidin 3-glucoside and cyanidin 3-glucoside levels, accompanied by consistent flavonoid pathway gene expression levels. FaMYB10 over-expression showed opposite FaMYB10 RNAi phenotypes, particularly cyanidin 3-glucoside synthesis by FaMYB10, which was correlated with FaF3'H transcript levels. These data provided evidence that light and ABA promoted FaMYB10 expression, resulting in anthocyanin accumulation via acceleration of flavonoid pathway gene expression. Finally, our results suggested FaMYB10 serves a role as a signal transduction mediator from light and ABA perception to anthocyanin synthesis in strawberry fruit. (Link to Publication Site) ● Publication site (DOI): 10.1007/s00425-014-2228-6 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25534946 ● Summary page in Scopus @ Elsevier: 2-s2.0-84925538505 (DOI: 10.1007/s00425-014-2228-6, PubMed: 25534946, Elsevier: Scopus)
5.	Qing-Ri Jin, Yukiko Bandou, Katsuyuki Miyawaki, Yosuke Shikama, Chisato Kosugi, Nanako Aki, Makoto Funaki and Sumihare Noji : Correlation of fibroblast growth factor 21 serum levels with metabolic parameters in Japanese subjects, The Journal of Medical Investigation : JMI, Vol.61, No.1.2, 28-34, 2014. (Summary) Since serum level of fibroblast growth factor 21 (FGF21) has been implicated as a potential biomarker for the early detection of the metabolic syndrome and type 2 diabetes, we examined how FGF21 serum levels are correlated with metabolic parameters in Japanese subjects. FGF21 levels were analyzed by enzyme-linked immunosorbent assays. Spearman's correlation and multiple stepwise regression analyses were used to examine the relationship between serum FGF21 and other factors. A Mann-Whitney U test was performed between the normal and high groups for triglycerides and systolic blood pressure (BP) respectively. By univariate correlation analysis, serum FGF21 levels were significantly associated with triglyceride levels, systolic BP, diastolic BP, pulse pressure, body mass index (BMI), age, fasting plasma glucose (FPG) levels, and total cholesterol levels. Multiple regression analysis (adjusted for age, gender, and BMI) showed that serum FGF21 levels were independently and significantly associated with triglyceride levels and systolic BP. Serum FGF21 levels were significantly higher in subjects with high triglyceride levels and high systolic BP compared with those who had normal triglyceride levels and normal systolic BP respectively. This study found that FGF21 levels might be a biomarker for some metabolic disorders associated with metabolic syndrome. (Keyword) Adult / Aged / Asian Continental Ancestry Group / Biological Markers / Blood Glucose / blood pressure / Body Mass Index / Cross-Sectional Studies / Female / Fibroblast Growth Factors / Humans / Male / Metabolic Syndrome X / Middle Aged / Regression Analysis / Triglycerides (Tokushima University Institutional Repository) ● Metadata: 109493 (Link to Publication Site) ● Publication site (DOI): 10.2152/jmi.61.28 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24705745 ● Search Scopus @ Elsevier (PMID): 24705745 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.61.28 (Tokushima University Institutional Repository: 109493, DOI: 10.2152/jmi.61.28, PubMed: 24705745)
6.	Y Kadomura-Ishikawa, Katsuyuki Miyawaki, Sumihare Noji and Akira Takahashi : Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits, Journal of Plant Research, Vol.126, No.6, 847-857, 2013. (Summary) Anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. In strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. However, the effects of different light qualities on anthocyanin accumulation in strawberry (Fragaria x ananassa, cv. Sachinoka) fruits remain elusive. In the present study, we showed the most efficient increase in anthocyanin content occurred by blue light irradiation. Light sensing at the molecular level was investigated by isolation of two phototropin (FaPHOT1 and FaPHOT2), two cryptochrome (FaCRY1 and FaCRY2), and two phytochrome (FaPHYA and FaPHYB) homologs. Expression analysis revealed only FaPHOT2 transcripts markedly increased depending on fruit developmental stage, and a corresponding increase in anthocyanin content was detected. FaPHOT2 knockdown resulted in decreased anthocyanin content; however, overexpression increased anthocyanin content. These findings suggested blue light induced anthocyanin accumulation, and FaPHOT2 may play a role in sensing blue light, and mediating anthocyanin biosynthesis in strawberry fruits. This is the first report to find a relationship between visible light sensing, and color development in strawberry fruits. (Keyword) Amino Acid Sequence / Anthocyanins / Antioxidants / Down-Regulation / Flavonoids / Fragaria / Fruit / Gene Expression Regulation, Plant / Gene Knockdown Techniques / Light / Molecular Sequence Data / Phototropins / Phylogeny / Pigmentation / Sequence Alignment / Up-Regulation (Tokushima University Institutional Repository) ● Metadata: 105905 (Link to Publication Site) ● Publication site (DOI): 10.1007/s10265-013-0582-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23982948 ● Summary page in Scopus @ Elsevier: 2-s2.0-84886728151 (Tokushima University Institutional Repository: 105905, DOI: 10.1007/s10265-013-0582-2, PubMed: 23982948, Elsevier: Scopus)
7.	Katsuyuki Miyawaki, Sachi Fukuoka, Yasuko Kadomura, Hirokazu Hamaoka, Taro Mito, Hideyo Ohuchi, Wilfried Schwab and Sumihare Noji : Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique, Plant Biotechnology, Vol.29, 271-278, 2012. (Summary) Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an Agrobacterium-mediated RNAi (AmRNAi) technique in post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of Fa chalcone synthase (Fa CHS), Fa chalcone isomerase (Fa CHI), and Fa flavonoid 3 -hydroxylase orthologues (Fa F3 H) in post-harvest fruit. Fa CHS and Fa F3 H function was subsequently examined by performing AmRNAi with post-harvest fruit. Although reduction of light-induced Fa F3 H expression by AmRNAi resulted in no significant change in anthocyanin content, reduction of Fa CHS significantly decreased anthocyanin levels, and up-regulated Fa F3 H levels. Our results are consistent with previous data indicating that while CHS is required for anthocyanin accumulation during late stage strawberry fruit maturation, Fa F3 H is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit. (Keyword) Agrobacterium-mediated RNAi / chalcone synthase / flavonoid 3'-hydroxylase / light-induced pigmentation / strawberry fruit (Link to Publication Site) ● Publication site (DOI): 10.5511/plantbiotechnology.12.0406a (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390282679303628160 ● Search Scopus @ Elsevier (DOI): 10.5511/plantbiotechnology.12.0406a (DOI: 10.5511/plantbiotechnology.12.0406a, CiNii: 1390282679303628160)
8.	Noha Dabour, Tetsuya Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi and Sumihare Noji : Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor., Development Growth & Differentiation, Vol.53, No.7, 857-869, 2011. (Summary) A long-standing problem of developmental biology is how body size is determined. In Drosophila melanogaster, the insulin/insulin-like growth factor (I/IGF) and target of rapamycin (TOR) signaling pathways play important roles in this process. However, the detailed mechanisms by which insect body growth is regulated are not known. Therefore, we have attempted to utilize systemic nymphal RNA interference (nyRNAi) to knockdown expression of insulin signaling components including Insulin receptor (InR), Insulin receptor substrate (chico), Phosphatase and tensin homologue (Pten), Target of rapamycin (Tor), RPS6-p70-protein kinase (S6k), Forkhead box O (FoxO) and Epidermal growth factor receptor (Egfr) and observed the effects on body size in the Gryllus bimaculatus cricket. We found that crickets treated with double-stranded RNA (dsRNA) against Gryllus InR, chico, Tor, S6k and Egfr displayed smaller body sizes, while Gryllus FoxO nyRNAi-ed crickets exhibited larger than normal body sizes. Furthermore, RNAi against Gryllus chico and Tor displayed slow growth and RNAi against Gryllus chico displayed longer lifespan than control crickets. Since no significant difference in ability of food uptake was observed between the Gryllus chico(nyRNAi) nymphs and controls, we conclude that the adult cricket body size can be altered by knockdown of expressions of Gryllus InR, chico, Tor, S6k, FoxO and Egfr by systemic RNAi. Our results suggest that the cricket is a promising model to study mechanisms underlying controls of body size and life span with RNAi methods. (Keyword) Amino Acid Sequence / Animals / Body Size / Cloning, Molecular / Female / Forkhead Transcription Factors / Gene Expression Regulation, Developmental / Genes, Insect / Gryllidae / Insect Proteins / Insulin / Longevity / Male / Molecular Sequence Data / Nymph / RNA Interference / Receptor, Epidermal Growth Factor / Receptor, Insulin / Signal Transduction / TOR Serine-Threonine Kinases (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1440-169X.2011.01291.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21777227 ● Search Scopus @ Elsevier (PMID): 21777227 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1440-169X.2011.01291.x (DOI: 10.1111/j.1440-169X.2011.01291.x, PubMed: 21777227)
9.	Kiyoshi Kunika, Toshihito Tanahashi, Shusuke Numata, Shu-ichi Ueno, Tetsuro Ohmori, Naoto Nakamura, Kazue Tsugawa, Katsuyuki Miyawaki, Maki Moritani, Hiroshi Inoue and Mitsuo Itakura : Common coding variant in the TCF7L2 gene and study of the association with type 2 diabetes in Japanese subjects, Journal of Human Genetics, Vol.53, No.11-12, 972-982, 2008. (Summary) Genetic variants of the transcription factor 7-like 2 (TCF7L2) gene affect the risk of type 2 diabetes in populations with multiple ethnic groups. However, a comprehensive survey of this gene has not been done for a Japanese population. Thus, we conducted this gene-based association study, in which the common genetic variants were analyzed. Using 24 Japanese type 2 diabetic subjects, we first screened a 9.5 kb region, which included the entire coding sequence, to assess potential functional variants of TCF7L2. Sequencing revealed a common coding variant (Pro477Thr) in exon 14 of TCF7L2 that was not enrolled in the public SNP database. Nineteen SNPs and the microsatellite DG10S478 were genotyped across the gene in 2,877 unrelated Japanese subjects. This independent screen identified the previously reported rs7903146 with a strongest association (allele P = 0.0001, odds ratio = 1.59 [95% confidence interval 1.25-2.01]), but there was no significant association between Pro477Thr and type 2 diabetes (allele P = 0.64). Expression of the Pro477Thr variant did not alter TCF7L2 expression in 30 lymphoblast cells. Although a genotypic effect of Pro477Thr on expression of TCF7L2 was not apparent, Pro477Thr was identified as a common variant of TCF7L2 in 2,877 Japanese subjects. Further functional studies are required to determine the possible effect of this coding variant on type 2 diabetes. (Keyword) Adult / Case-Control Studies / Codon / Diabetes Mellitus, Type 2 / Female / Genetic Predisposition to Disease / Genotype / Humans / Japan / Male / Middle Aged / Polymerase Chain Reaction / Polymorphism, Single Nucleotide / Risk Factors / TCF Transcription Factors / Transcription Factor 7-Like 2 Protein (Link to Publication Site) ● Publication site (DOI): 10.1007/s10038-008-0339-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19012045 ● Search Scopus @ Elsevier (PMID): 19012045 ● Search Scopus @ Elsevier (DOI): 10.1007/s10038-008-0339-2 (DOI: 10.1007/s10038-008-0339-2, PubMed: 19012045)
10.	Katsuyuki Miyawaki, Hiroshi Inoue, Parvaneh Keshavarz, Kuniko Mizuta, Aya Sato, Yukiko Yamashita, Maki Moritani, Kiyoshi Kunika, Toshihito Tanahashi and Mitsuo Itakura : Transgenic expression of a mutated cyclin-dependent kinase 4 (CDK4/R24C) in pancreatic beta-cells prevents progression of diabetes in db/db mice., Diabetes Research and Clinical Practice, Vol.82, No.1, 33-41, 2008. (Summary) In an attempt to rectify the hyperglycemic state in obese insulin resistant db/db mice, a transgenic line was generated (db/db-CDK4(R24C)) that expresses a constitutively active form of cyclin-dependent kinase 4 (CDK4/R24C) under the control of the insulin promoter. Compared with non-transgenic db/db littermates, adult db/db-CDK4(R24C) mice show near-complete glycemic normalization and improved plasma lipid concentrations, but are also more susceptible to weight gain and have significantly lower plasma adiponection levels. They have striking islet hypertrophy and beta-cell hyperplasia, and retain an insulin secretory response during the glucose tolerance test. We examined the expression of several key regulatory transcription factor genes involved in lipid and glucose metabolism in insulin target tissues of db/db-CDK4(R24C) as well as db/db mice, and found that the expression levels of members of the peroxisome proliferator-activated receptor (PPAR) family are highly associated with metabolic alterations in a gene- and tissue-specific manner. We show for the first time that the Ppar-delta in skeletal muscle and white adipose tissues is transcriptionally down-regulated in db/db mice. The db/db-CDK4(R24C) mice present a novel model of leptin-resistant obesity with compensatory hyperinsulinemia and normalized blood glucose levels, and thus may be useful for future studies that aim to dissect relationships between insulin and leptin signaling. (Keyword) Adiponectin / Animals / Blood Glucose / Body Weight / Cyclin-Dependent Kinase 4 / Diabetes Mellitus / Disease Progression / Female / Humans / Insulin Resistance / Insulin-Secreting Cells / Lipids / Liver / Male / Mice / Mice, Inbred C57BL / Mice, Mutant Strains / Mice, Transgenic / Mutation / Nuclear Proteins / Peroxisome Proliferator-Activated Receptors / Reverse Transcriptase Polymerase Chain Reaction / Sterol Regulatory Element Binding Proteins / Transcription Factors / Triglycerides (Link to Publication Site) ● Publication site (DOI): 10.1016/j.diabres.2008.06.014 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18678431 ● Search Scopus @ Elsevier (PMID): 18678431 ● Search Scopus @ Elsevier (DOI): 10.1016/j.diabres.2008.06.014 (DOI: 10.1016/j.diabres.2008.06.014, PubMed: 18678431)
11.	Toshihito Tanahashi, Keiko Shinohara, Parvaneh Keshavarz, Yuka Nagasaki, Katsuyuki Miyawaki, Kiyoshi Kunika, Maki Moritani, Naoto Nakamura, Toshikazu Yoshikawa, Hiroshi Shiota, Hiroshi Inoue and Mitsuo Itakura : The association of genetic variants in Krüppel-like factor 11 and Type 2 diabetes in the Japanese population., Diabetic Medicine, Vol.25, No.1, 19-26, 2008. (Summary) Krüppel-like factor 11 (KLF11) is a transcriptional factor of the zinc finger domain family that regulates the expression of insulin. In North European populations, its common functional variant Q62R (rs35927125) is a strong genetic factor for Type 2 diabetes (P = 0.00033, odds ratio for G allele = 1.29, 95% CI 1.12-1.49). We examined the contribution of KLF11 variants to the susceptibility to Type 2 diabetes in a Japanese population. By re-sequencing Japanese individuals (n = 24, partly 96), we screened all four exons, exon/intron boundaries and flanking regions of KLF11. Verified single nucleotide polymorphisms (SNPs) were genotyped in 731 initial samples (369 control and 362 case subjects). Subsequently, we tested for association in 1087 samples (524 control and 563 case subjects), which were collected in different districts of Japan from the initial samples. We identified eight variants, including a novel A/C variant on intron 3, but no mis-sense mutations. In an association study, we failed to find any significant result of SNPs (minor allele frequency 8.2-46.2%) after correcting for multiple testing. Similarly, no haplotypes were associated with Type 2 diabetes. It is notable that the G allele in rs35927125 was completely absent in 1818 Japanese individuals. Genetic variants in KLF11 are unlikely to have a major effect of Type 2 diabetes in the Japanese population, although they were significantly associated in North European populations. These observations might help to determine the role of KLF11 variants in Type 2 diabetes in different populations. (Keyword) Adult / analysis of variance / Asian Continental Ancestry Group / Cell Cycle Proteins / Diabetes Mellitus, Type 2 / female / Gene Frequency / Genetic Predisposition to Disease / Genotype / Humans / insulin / Japan / Linkage Disequilibrium / male / Middle Aged / Repressor Proteins (Tokushima University Institutional Repository) ● Metadata: 118435 (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1464-5491.2007.02315.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18199129 ● Search Scopus @ Elsevier (PMID): 18199129 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1464-5491.2007.02315.x (Tokushima University Institutional Repository: 118435, DOI: 10.1111/j.1464-5491.2007.02315.x, PubMed: 18199129)
12.	Yukiko Yamashita, Hiroshi Inoue, Keshavarz Parvaneh, Katsuyuki Miyawaki, Yuka Nagasaki, Maki Moritani, Kiyoshi Kunika, Naoto Nakamura, Toshikazu Yoshikawa, Natsuo Yasui, Hiroshi Shiota, Toshihito Tanahashi and Mitsuo Itakura : SNPs in the KCNJ11-ABCC8 gene locus are associated with type 2 diabetes and blood pressure levels in the Japanese population., Journal of Human Genetics, Vol.52, No.10, 781-793, 2007. (Summary) Many genetic association studies support a contribution of genetic variants in the KCNJ11-ABCC8 gene locus to type 2 diabetes (T2D) susceptibility in Caucasians. In non-Caucasian populations, however, there have been only a few association studies, and discordant results were obtained. Herein, we selected a total of 31 SNPs covering a 211.3-kb region of the KCNJ11-ABCC8 locus, characterized the patterns of linkage disequilibrium (LD) and haplotype structure, and performed a case-control association study in a Japanese population consisting of 909 T2D patients and 893 control subjects. We found significant associations between eight SNPs, including the KCNJ11 E23K and ABCC8 S1369A variants, and T2D. These disease-associated SNPs were genetically indistinguishable because of the presence of strong LD, as found previously in Caucasians. For the KCNJ11 E23K variant, the most significant association was obtained under a dominant genetic model (OR 1.32, 95% CI 1.09-1.60, P = 0.004). A meta-analysis of East Asian studies, comprising a total of 3,357 T2D patients (77.4% Japanese) and 2,836 control subjects (77.8% Japanese), confirmed the significant role of the KCNJ11 E23K variant in T2D susceptibility. Furthermore, we found evidence suggesting that the KCNJ11 E23K genotype is independently associated with higher blood-pressure levels. (Keyword) ATP-Binding Cassette Transporters / Aged / Asian Continental Ancestry Group / blood pressure / Diabetes Mellitus, Type 2 / female / Humans / Japan / male / Middle Aged / Polymorphism, Single Nucleotide / Potassium Channels / Potassium Channels, Inwardly Rectifying / Receptors, Drug (Link to Publication Site) ● Publication site (DOI): 10.1007/s10038-007-0190-x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17823772 ● CiNii @ National Institute of Informatics (CRID): 1521417754939063936 ● Summary page in Scopus @ Elsevier: 2-s2.0-34848895423 (DOI: 10.1007/s10038-007-0190-x, PubMed: 17823772, CiNii: 1521417754939063936, Elsevier: Scopus)
13.	Kuniko Mizuta, Satoshi Tsutsumi, Hiroshi Inoue, Yukiko Yamashita, Katsutoshi Miyatake, Katsuyuki Miyawaki, Sumihare Noji, Nobuyuki Kamata and Mitsuo Itakura : Molecular characterization of GDD1/TMEM16E, the gene product responsible for autosomal dominant gnathodiaphyseal dysplasia., Biochemical and Biophysical Research Communications, Vol.357, No.1, 126-132, 2007. (Summary) The human GDD1/TMEM16E gene has been found to be mutated in gnathodiaphyseal dysplasia, an unusual skeletal syndrome with autosomal dominant inheritance. The molecular and biochemical function(s) of GDD1 protein has not yet been elucidated. In this study, we examined the murine GDD1 gene expression pattern during embryonic development, and characterized the cellular and tissue localizations of its gene product using a GDD1-specific antibody. In the developing embryos, GDD1 mRNA expression was principally associated with differentiating and developing somites, with a highly complex spatiotemporal pattern that involved the myotomal and sclerotomal lineages of somites. Biochemical studies indicated that GDD1 protein is an integral membrane glycoprotein that resides predominantly in intracellular vesicles. Immunohistochemical analysis showed a high level of murine GDD1 protein expression in cardiac and skeletal muscle tissues, and in growth-plate chondrocytes and osteoblasts in bone. These observations suggest diverse cellular role(s) of GDD1 in the development of musculoskeletal system. (Keyword) Animals / Bone Diseases / Chromosome Disorders / Embryonic Development / Genes, Dominant / Membrane Proteins / Mice / Muscle, Skeletal / myocardium / Organ Specificity / Tissue Distribution (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2007.03.108 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17418107 ● Summary page in Scopus @ Elsevier: 2-s2.0-34147113231 (DOI: 10.1016/j.bbrc.2007.03.108, PubMed: 17418107, Elsevier: Scopus)
14.	Taro Mito, Chiharu Kobayashi, Isao Sarashina, H Zhang, Wakako Shinahara, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi and Sumihare Noji : even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera)., Developmental Biology, Vol.303, No.1, 202-213, 2007. (Keyword) Amino Acid Sequence / Animals / Base Sequence / Body Patterning / Evolution, Molecular / Gene Expression Profiling / Gene Expression Regulation / Gryllidae / Homeodomain Proteins / In Situ Hybridization / Molecular Sequence Data / Phylogeny / RNA Interference / Sequence Alignment / Sequence Analysis, DNA / Transcription Factors (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ydbio.2006.11.003 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17174947 ● Search Scopus @ Elsevier (PMID): 17174947 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2006.11.003 (DOI: 10.1016/j.ydbio.2006.11.003, PubMed: 17174947)
15.	Yukiko Yamashita, Hiroshi Inoue, Shuhei Kawakami, Katsuyuki Miyawaki, Tatsuro Miyamoto, Kuniko Mizuta and Mitsuo Itakura : Expression and distribution of Gpr119 in the pancreatic islets of mice and rats: Predominant localization in pancreatic polypeptide-secreting PP-cells., Biochemical and Biophysical Research Communications, Vol.351, No.2, 474-480, 2006. (Summary) The GPR119 was recently shown to be activated by oleoylethanolamide (OEA), a naturally occurring bioactive lipid with hypophagic and anti-obesity effects. In this study, we have cloned and characterized its murine counterpart, Gpr119. The full-length cDNA contained an open reading frame of 1008bp encoding a 335-amino acid protein. The genomic organization of Gpr119 was unique, having a 3'-untranslated second exon that was also involved in an alternative splicing event. Gene expression analyses confirmed its specific expressions in pancreatic islets and two endocrine cell-lines, MIN6 and alphaTC1. Immunohistochemistry and double-immunofluorescence studies using a specific antibody revealed the predominant Gpr119 localization in pancreatic polypeptide (PP)-cells of islets. No definitive evidence of Gpr119-immunoreactivity in adult beta- or alpha-cells was obtained. The Gpr119 mRNA levels were elevated in islets of obese hyperglycemic db/db mice as compared to control islets, suggesting a possible involvement of this receptor in the development of obesity and diabetes. (Keyword) 3' Untranslated Regions / Alternative Splicing / Animals / Base Sequence / Cell Line / Cloning, Molecular / Exons / Hyperglycemia / Islets of Langerhans / Male / Mice / Mice, Obese / Molecular Sequence Data / Open Reading Frames / Pancreatic Polypeptide-Secreting Cells / RNA, Messenger / Rats / Receptors, G-Protein-Coupled / Recombinant Fusion Proteins (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bbrc.2006.10.076 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17070774 ● Summary page in Scopus @ Elsevier: 2-s2.0-33750520737 (DOI: 10.1016/j.bbrc.2006.10.076, PubMed: 17070774, Elsevier: Scopus)
16.	Taro Mito, Haruko Okamoto, Wakako Shinahara, Yohei Shinmyo, Katsuyuki Miyawaki, Hideyo Ohuchi and Sumihare Noji : Kruppel acts as a gap gene regulating expression of hunchback and even-skipped in the intermediate germ cricket Gryllus bimaculatus., Developmental Biology, Vol.294, No.2, 471-481, 2006. (Keyword) Animals / Biological Evolution / Body Patterning / Embryo, Nonmammalian / Gene Expression Regulation, Developmental / Gryllidae / Homeodomain Proteins / In Situ Hybridization / Insect Proteins / Kruppel-Like Transcription Factors / Phenotype / RNA Interference / signal transduction (Link to Publication Site) ● Publication site (DOI): 10.1016/j.ydbio.2005.12.057 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16616119 ● Search Scopus @ Elsevier (PMID): 16616119 ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2005.12.057 (DOI: 10.1016/j.ydbio.2005.12.057, PubMed: 16616119)
17.	Honghie Zhang, Yohei Shinmyo, Taro Mito, Katsuyuki Miyawaki, Isao Sarashina, Hideyo Ohuchi and Sumihare Noji : Expression patterns of the homeotic genes Scr, Antp, Ubx, and abd-A during embryogenesis of the cricket Gryllus bimaculatus, Gene Expression Patterns, Vol.5, No.4, 491-502, 2005. (Summary) コオロギGryllus bimaculatusのホメオティク遺伝子である Scr, Antp, Ubx, と abd-A の発現パターンを胚において調べた．その結果，発現パターンは基本的にショウジョウバエの対応する遺伝子と類似していたが，まったく同じではなかった．Scrは顎部から第1胸部体節から後部にかけてと脚に, Antpは第2胸部体節から後部にかけてとその脚に, Ubxは第3胸部体節から後部とその脚に, abd-Aは腹部体節に発現していることを発見した． (Keyword) Cricket / Gryllus bimaculatus / Scr / Antp / Ubx / abd-A / Expression patterns / Drosophila melanogaster (Link to Publication Site) ● Publication site (DOI): 10.1016/j.modgep.2004.12.006 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15749077 ● Search Scopus @ Elsevier (PMID): 15749077 ● Search Scopus @ Elsevier (DOI): 10.1016/j.modgep.2004.12.006 (DOI: 10.1016/j.modgep.2004.12.006, PubMed: 15749077)
18.	Taro Mito, Isao Sarashina, Hongjie Zhang, Akihiro Iwahashi, Haruko Okamoto, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi and Sumihare Noji : Non-canonical functions of hunchback in segment patterning of the intermediate germ cricket Gryllus bimaculatus, Development, Vol.132, No.9, 2069-2079, 2005. (Summary) コオロギのhunchbackを単離し，その遺伝子発現パターンと機能をRNAi法により解析した．hunchbackは顎の体節に発現し，その後後部成長領域にも発現した．RNAiの解析により，hunchbackは顎部·胸部のアイデンティティーに関係しており，ノックダウンにより体節のトランスフォーメーションが生じることがわかった．また，発生初期の解析から，トランスフォーメーションだけでなく，一部体節が欠失していることも発見した．これらはショウジョウバエやカメムシと異なるhunchbackの役割であることを発見した． (Keyword) hunchback / cricket / Development (Link to Publication Site) ● Publication site (DOI): 10.1242/dev.01784 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15788457 ● Search Scopus @ Elsevier (PMID): 15788457 ● Search Scopus @ Elsevier (DOI): 10.1242/dev.01784 (DOI: 10.1242/dev.01784, PubMed: 15788457)
19.	Isao Sarashina, Taro Mito, Michiko Saito, Hiroyuki Uneme, Katsuyuki Miyawaki, Yohei Shinmyo, Hideyo Ohuchi and Sumihare Noji : Location of micropyles and early embryonic development of the two-spotted cricket Gryllus bimaculatus (Insecta, Orthoptera), Development Growth & Differentiation, Vol.47, No.2, 99-108, 2005. (Summary) Early embryogenesis of the two-spotted cricket Gryllus bimaculatus was examined by scanning electron microscopy and several fluorescence staining methods, with special reference to these four issues: (i) the location of micropyles; (ii) the transfer of the female pronucleus following meiosis; (iii) the timing of cellularization; and (iv) the process of the germ primordium formation. Between two and four micropyles lie in the mid-ventral region of the egg. The egg nucleus is at the mid-dorsal periphery of the new laid egg, and meiosis resumes and is completed there. The female pronucleus moves to the mid-ventral side, and fertilization occurs there. Energid starts to proliferate and migrates to the periphery of the egg, initiating blastoderm formation. Actin caps surround each superficial nucleus. Cellularization occurs during the blastoderm stage. At a late blastoderm stage, nuclei aggregate in both the posterolateral patch-like regions of the egg to form a germ primordium. The germ primordium looks like a pair of dumbbells. Both the patches shift towards the ventral side and fuse into a germ primordium. The germ primordium contracts to produce a clearly delineated germ band. Observations on distribution patterns of F-actin indicate that, all through the process, the germ primordium retains that unity, and is not separated into two parts. (Keyword) Actin Cytoskeleton / Animals / Blastoderm / Gryllidae / Meiosis / Microscopy, Electron, Scanning / Microscopy, Fluorescence / Mitosis / Ovum / Sperm-Ovum Interactions (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1440-169x.2005.00786.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15771629 ● CiNii @ National Institute of Informatics (CRID): 1520009408743805312 ● Search Scopus @ Elsevier (PMID): 15771629 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1440-169x.2005.00786.x (DOI: 10.1111/j.1440-169x.2005.00786.x, PubMed: 15771629, CiNii: 1520009408743805312)
20.	Yohei Shinmyo, Taro Mito, Taro Matsushita, Isao Sarashina, Katsuyuki Miyawaki, Hideyo Ohuchi and Sumihare Noji : caudal is required for gnathal and thoracic patterning and for posterior elongation in the intermediate-germband cricket Gryllus bimaculatus, Mechanisms of Development, Vol.122, No.2, 231-239, 2005. (Summary) Although the molecular mechanisms directing anteroposterior patterning of the Drosophila embryo (long-germband mode) are well understood, how these mechanisms were evolved from an ancestral mode of insect embryogenesis remains largely unknown. In order to gain insight into mechanisms of evolution in insect embryogenesis, we have examined the expression and function of the orthologue of Drosophila caudal (cad) in the intermediate-germband cricket Gryllus bimaculatus. We observed that a posterior (high) to anterior (low) gradient in the levels of Gryllus bimaculatus cad (Gb' cad) transcript was formed in the early-stage embryo, and then Gb' cad was expressed in the posterior growth zone until the posterior segmentation was completed. Reduction of Gb' cad expression level by RNA interference resulted in deletion of the gnathum, thorax, and abdomen in embryos, remaining only anterior head. We found that the gnathal and thoracic segments are formed by Gb' cad probably through the transcriptional regulation of gap genes including Gb' hunchback and Gb' Kruppel. Furthermore, Gb' cad was found to be involved in the posterior elongation, acting as a downstream gene in the Wingless/Armadillo signalling pathways. These findings indicate that Gb' cad does not function as it does in Drosophila, suggesting that regulatory and functional changes of cad occurred during insect evolution. Since Wnt/Cdx pathways are involved in the posterior patterning of vertebrates, such mechanisms may be conserved in animals that undergo sequential segmentation from the posterior growth zone. (Keyword) development / cricket / caudal (Link to Publication Site) ● Publication site (DOI): 10.1016/j.mod.2004.10.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15652710 ● Search Scopus @ Elsevier (PMID): 15652710 ● Search Scopus @ Elsevier (DOI): 10.1016/j.mod.2004.10.001 (DOI: 10.1016/j.mod.2004.10.001, PubMed: 15652710)
21.	Yoshiko Inoue, Katsuyuki Miyawaki, Taiki Terasawa, Kyoko Matsushima, Yohei Shinmyo, Nao Niwa, Taro Mito, Hideyo Ohuchi and Sumihare Noji : Expression patterns of dachshund during head development of Gryllus bimaculatus (cricket), Gene Expression Patterns, Vol.4, No.6, 725-731, 2004. (Summary) コオロギのdachshundの脳および眼の発生における発現パターンを調べた．脳においては，将来キノコ体の細胞になる細胞に発現していることを発見した．眼の原基においては，その後部に発現し，個眼が形成される前に発現しているこを発見した．これらの結果をショウジョウバエの dachsfundの発現と比較したところ，基本的な発現パターンは保存されていたが，発現のタイミングが異なることがわかった． (Keyword) cricket / dachshund / head / expression patterns (Link to Publication Site) ● Publication site (DOI): 10.1016/j.modgep.2004.03.010 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15465496 ● Summary page in Scopus @ Elsevier: 2-s2.0-4744347091 (DOI: 10.1016/j.modgep.2004.03.010, PubMed: 15465496, Elsevier: Scopus)
22.	Yohei Shinmyo, Taro Mito, Takashi Matsushita, Isao Sarashina, Katsuyuki Miyawaki, Hideyo Ohuchi and Sumihare Noji : piggyBac-Mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus (cricket)., Development Growth & Differentiation, Vol.46, No.4, 343-349, 2004. (Summary) トランスジェニックコオロギを作製するために，トランスポゾンの利用を検討した．トランスポゾンとしてpiggyBacを選び，そのmRNAと GFPをコードするドナーベクターをコオロギの卵内に共インジェクションして，GFPの蛍光を観察した．その結果，胚にGFPが観察できたことから，piggyBacが卵内で活性があり，ドナーのGFP遺伝子をコオロギ染色体に転移できることがわかった．しかし，次世代のコオロギににGFPを発現するものが得られたなかったことから，ジャームラインには遺伝子が導入されていないことが示唆された． (Keyword) cricket / transformation / piggyBac (Link to Publication Site) ● Publication site (DOI): 10.1111/j.1440-169x.2004.00751.x (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15367202 ● CiNii @ National Institute of Informatics (CRID): 1520853832274834432 ● Search Scopus @ Elsevier (PMID): 15367202 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1440-169x.2004.00751.x (DOI: 10.1111/j.1440-169x.2004.00751.x, PubMed: 15367202, CiNii: 1520853832274834432)
23.	Katsuyuki Miyawaki, Taro Mito, Isao Sarashina, Hongjie Zhang, Yohei Shinmyo, Hideyo Ohuchi and Sumihare Noji : Involvement of Wingless/Armadillo signaling in the posterior sequential segmentation in the cricket, Gryllus bimaculatus (Orthoptera), as revealed by RNAi analysis, Mechanisms of Development, Vol.121, No.2, 119-130, 2004. (Summary) コオロギの初期胚ではwinglessが後部増殖領域に発現している．その役割を解明する目的でRNAiによる遺伝子のノックダウンを試みたが，表現型に変化がなかった．そこで，winglessの細胞内シグナル経路の成分の一つであるarmadilloに着目して解析を行なった．その結果，armadilloのノックダウンにより腹部体節が欠損することがわかった．これらのことから，コオロギ胚の後部の伸長にwingless/arimadilloシグナルが関与していることが示唆された． (Keyword) Amino Acid Sequence / Animals / Base Sequence / Body Patterning / DNA, Complementary / Gene Expression Regulation, Developmental / Genes, Insect / Gryllidae / Insect Proteins / Molecular Sequence Data / RNA Interference / Sequence Homology, Amino Acid / Signal Transduction (Link to Publication Site) ● Publication site (DOI): 10.1016/j.mod.2004.01.002 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15037314 ● Search Scopus @ Elsevier (PMID): 15037314 ● Search Scopus @ Elsevier (DOI): 10.1016/j.mod.2004.01.002 (DOI: 10.1016/j.mod.2004.01.002, PubMed: 15037314)

Review, Commentary:

1.	Katsuyuki Miyawaki, Hiroshi Inoue and Mitsuo Itakura : メタボリックシンドローム-病因解明と予防・治療の最新戦略-, 日本臨床増刊号, Vol.64, 702-706, 2006.

Proceeding of International Conference:

1.	Izumi Takuto, Aihara Mutsumi, Retsuo Kawakami, Akihiro Shirai, Urakami Tomona, Katsuyuki Miyawaki and Takashi Mukai : Bactericidal Effects of Nonequilibrium Atmospheric-Pressure Plasma Jet on Hydroponic Nutrient Solutions, Proceedings of International Symposium of Dry Process 2021, 129-130, Tokyo, Nov. 2021.
2.	Katsuyuki Miyawaki : Disinfection system using UV light-emitting diodes~plant growth control by LED~, 宇宙栄養研究センター設立記念シンポジウム, Nov. 2018.
3.	Tsunedomi Akari, Masamura Akinori, Mutsumi Nakahashi, Nishisaka Risa, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Disinfection system with UVA-LED for hydroponic nutrient solution, 6th FEMS Microbiology Congress, Jun. 2015.
4.	Makoto Mizunami, T Takahashi, A Hamada, Katsuyuki Miyawaki, Y Matsumoto, Taro Mito and Sumihare Noji : Systemic RNA interference for the study of long-term memory formation in the cricket, The 2nd International Conference on the Cricket / RNAi Symposium, tokushima,Japan, Mar. 2012.
5.	Taro Mito, Katsuyuki Miyawaki, Y Shinmyo, Hideyo Ohuchi and Sumihare Noji : Genome-wide RNAi in the cricket Gryllus bimaculactus as a new model system to study gene functions, 10th Anniversary of Kazusa DNA Research Institute 14th International Workshop, Japan, Oct. 2004. (Summary) コオロギにおいて，卵のRNAi, 幼虫のRNAi，親RNAiにより簡単に遺伝子の機能解析ができる事を示した．このゲノムワイドなRNA干渉法により，コオロギの全遺伝子の機能が比較的簡単に調べる事ができる．
6.	Sumihare Noji, Taro Mito, Y Tanaka, Katsuyuki Miyawaki, (名) Shinmyo and Hideyo Ohuchi : Involvement of Wnt and EGF signaling systems in regeneraion of cricket legs, 8th international Conference Limb Development and Regeneration, 47, Dundee, UK, Jul. 2004.

Proceeding of Domestic Conference:

1.	HIROKI Matsuki, MINORI Suzuki, KONO Madoka, ANAYT ULLA, Kazuaki Mawatari, Akira Takahashi, Katsuyuki Miyawaki, Takahito Watanabe, Taro Mito, Takayoshi Kuriki and Takeshi Nikawa : コオロギの抗筋萎縮作用について, 第77回日本栄養・食糧学会大会, May 2023.
2.	井上慎太郎, Takahito Watanabe, HAMAGUCHI Taiki, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito : コオロギをモデルとした体色パターン制御の分子メカニズムの解析, 第67回日本応用動物昆虫学会, Mar. 2023.
3.	HAMAGUCHI Taiki, 井上慎太郎, Katsuyuki Miyawaki, Akira Takahashi, Takeshi Nikawa, Yoshiyasu Ishimaru, Sumihare Noji, Takahito Watanabe and Taro Mito : フタホシコオロギにおける色素合成に関わる遺伝子の機能解析, 第45回日本分子生物学会, Dec. 2022.
4.	HIROKI Matsuki, Minori Yamasaki, KONO Madoka, NAKANO Wataru, (名) ANAYTULLA, Akira Takahashi, Katsuyuki Miyawaki, Takahito Watanabe, Taro Mito, Takayoshi Kuriki and Takeshi Nikawa : コオロギの抗筋萎縮作用について, 第76回日本栄養・食糧学会大会, Jun. 2022.
5.	Yoshihiko Ueno, Eiji Sakuradani, Yosuke Seki, Maiko Kamioka, Chikako Asada, Tetsuya Akamatsu, Katsuyuki Miyawaki, Yoshihiro Uto and Tamotsu Tanaka : 一般選抜後期日程における入学辞退率改善の取り組みーー徳島大学B学部の事例からーー, 第17回大学教育カンファレンスin徳島, Jan. 2022.
6.	泉匠人, Retsuo Kawakami, Mutsumi Aihara, Takumi Matsumura, Akihiro Shirai, Katsuyuki Miyawaki and Takashi Mukai : 植物養液への大気圧低温空気プラズマジェット殺菌効果, 2021年度応用物理・物理系学会中国四国支部合同学術講演会, 40, Jul. 2021.
7.	Takuto Izumi, Retsuo Kawakami, Mutsumi Aihara, Akihiro Shirai, Katsuyuki Miyawaki and Takashi Mukai : 大気圧低温プラズマジェット照射による植物養液殺菌効果, 第68回春季応用物理学会学術講演会, 07-070, Mar. 2021.
8.	泉匠人, Retsuo Kawakami, Mutsumi Aihara, Akihiro Shirai, Katsuyuki Miyawaki and Takashi Mukai : 植物養液への非平衡大気圧空気プラズマジェット照射効果, 2020年度応用物理・物理系学会中国四国支部合同学術講演会, 10, Aug. 2020.
9.	Masanobu Haraguchi, Takahiro Kitada, Masao Nagase, Takeshi Yasui, Yohsuke Kinouchi, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : 2019年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み, LED総合フォーラム2020in徳島, 75-76, Feb. 2020. (Keyword) LEDライフイノベーション / LED / テラヘルツ
10.	Takeshi Nikawa, Akira Takahashi and Katsuyuki Miyawaki : 機能性宇宙食, 第63回宇宙科学技術連合講演会, Nov. 2019.
11.	Reiko Nakao, Katsuyuki Miyawaki, Yoshihiro Deguchi, Akira Takahashi and Takeshi Nikawa : 宇宙栄養・食糧関連技術の開発とGatewayへの期待, 国際宇宙探査ワークショップ(その2), Mar. 2019.
12.	Katsuyuki Miyawaki : UVA-LEDを用いた植物工場の循環養液の殺菌方法について, 平成30年度LED応用技術セミナー, Feb. 2019.
13.	Katsuyuki Miyawaki : 植物工場における循環式水耕栽培の殺菌, 第11回日本カンピロバクター研究会総会, Dec. 2018.
14.	Akari Tsunedomi, Takaaki Shimohata, Sakie Nagata, Sachie Amano, Mutsumi Nakahashi, Yumi Harada, Takashi Uebanso, Kazuaki Mawatari, Katsuyuki Miyawaki, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有用性について, 第37回日本食品微生物学会学術総会, Sep. 2016.
15.	Akari Tsunedomi, Takaaki Shimohata, Marin Gotoh, Sachie Amano, Mutsumi Nakahashi, 原田優美, Takashi Uebanso, Kazuaki Mawatari, Katsuyuki Miyawaki, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : UVA-LED殺菌システムによるCampylobacter jejuni食中毒の予防, 第70回日本栄養・食糧学会大会, May 2016.
16.	上田梨紗, 阿部千尋, 石原諒典, Takahito Watanabe, Sigeo Sugano, Katsuyuki Miyawaki, Sumihare Noji, Yuriko Osakabe and Keishi Osakabe : 高効率 CRISPR/Cas9による SlIAA9 ノックアウトトマトの作出, 第57回日本植物生理学会大会, Mar. 2016.
17.	上田梨紗, 石原諒典, 阿部千尋, Takahito Watanabe, Sigeo Sugano, Katsuyuki Miyawaki, Sumihare Noji, Yuriko Osakabe and Keishi Osakabe : CRISPR/Cas9によるトマトIAA9 遺伝子を標的としたゲノム編集技術の確立, 第38回日本分子生物学会年会, Dec. 2015.
18.	Katsuyuki Miyawaki, 常冨愛香里, 後藤茉凜, 正村彰規, Akira Takahashi and Sumihare Noji : 完全人工光型植物工場におけるイチゴ苗大量生産システムの開発, 園芸学会, Sep. 2015. (Keyword) 完全人工光型植物工場 / イチゴ / UVA-LED殺菌
19.	Akari Tsunedomi, Akinori Masamura, Mutsumi Nakahashi, Risa Nishisaka, Marin Gotoh, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : 植物工場の養液殺菌システムの開発, 第251回徳島医学会学術集会, Aug. 2015.
20.	Katsuyuki Miyawaki, Yasuko Kadomura-Ishikawa, Keishi Osakabe and Sumihare Noji : Functional analyses of R2R3 MYB transcription factors in anthocyanin biosynthesis pathway of the strawberry, 第56回日本植物生理学会, Mar. 2015.
21.	Akari Tsunedomi, Mutsumi Nakahashi, Risa Nishisaka, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : 水耕栽培用養液の還流型殺菌システムの開発, 第67回日本細菌学会中国・四国支部総会, Oct. 2014.
22.	Katsuyuki Miyawaki, 平田翔悟, 石川寧子, 三戸太郎, 刑部敬史 and 野地澄晴 : アグロインフィルトレーション法を用いたF3'5'H過剰発現によるイチゴ花托の着色への影響, 第32回日本植物細胞分子生物学会, Aug. 2014.
23.	平田翔悟, 上田梨紗, Takahito Watanabe, Katsuyuki Miyawaki, 三戸太郎 and 野地澄晴 : Transcription Activator-Like Effector NucleasesによるIAA9ノックアウトトマト作製の試み, 第36回日本分子生物学会, Dec. 2013.
24.	Katsuyuki Miyawaki, 浜岡宏和, 角村寧子, 三戸太郎 and 野地澄晴 : LEDを利用したイチゴの生長と着色の促進法の開発, 日本生物環境工学会, Sep. 2012.
25.	Kentaro Irie, Yuri Sakaue, Sachi Fukuoka, Katsuyuki Miyawaki, 角村寧子, 広田恵介, Taro Mito, Hideyo Ohuchi and Sumihare Noji : アグロインフィルトレーション法を用いたRNAiによるイチゴ青色光受容体の機能解析, 第33回日本分子生物学会年会, 2010.
26.	Sachi Fukuoka, Yuri Sakaue, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi and Sumihare Noji : イチゴ「さちのか」におけるflavonoid 3'-hydoxylase (F3'H) 遺伝子の機能解析, 第33回日本分子生物学会年会, Dec. 2010.
27.	Noha Dabour, Tetsuy Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi and Sumihare Noji : Control of body size by chico and epidermal growth factor receptor, as revealed by systemic nymphal RNA interference in the cricket, 第43回日本発生生物学会年会, Jun. 2010.
28.	Maki Moritani, 山口裕加, 藤田由香, Toshihito Tanahashi, 国香清, Katsuyuki Miyawaki, Takeo Iwata, Hiroshi Inoue and Mitsuo Itakura : 3番染色体短腕の関連解析探索により見出した日本人2型糖尿病疾患感受性ENDOGL1遺伝子の機能解析, 第51回日本糖尿病学会年次学術集会, 2008.
29.	Katsuyuki Miyawaki, 佐藤綾, Yukiko Yamashita, Hiroshi Inoue and Mitsuo Itakura : 常時活性型Cdk4を発現する2型糖尿病マウス(db/dbマウス)における膵β細胞増殖による高血糖改善効果とその限界, 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会, 2007.
30.	佐藤綾, Yukiko Yamashita, Katsuyuki Miyawaki, Hiroshi Inoue and Mitsuo Itakura : 膵β細胞にCDK4を過剰発現させたdb/dbトランスジェニックマウスの解析, 日本糖尿病学会中国四国地方会第44回総会, 2006.

Et cetera, Workshop:

1.	Retsuo Kawakami, ICHIMURA Atsunori, Akihiro Shirai, Katsuyuki Miyawaki, 立木弥生, 吉田雅彦 and 福光秀之 : 405-nm LED照射とg-C3N4ナノシートによる果実鮮度保持効果, 次世代光フォーラム2023 in 徳島, 103-104, Jan. 2024.
2.	Retsuo Kawakami, Kohsuke Takagi, Akihiro Shirai, Katsuyuki Miyawaki, 立木弥生, 吉田雅彦 and 福光秀之 : 可視光LED照射したグラファイト状窒化炭素の鮮度保持効果, 次世代光フォーラム2023 in 徳島, 111-112, Feb. 2023.
3.	Katsuyuki Miyawaki : LED植物工場による植物の高機能化に関する産学連携のプロジェクト, 日本統合医療学会四国支部会報, No.15, 50-51, Dec. 2022.
4.	Masanobu Haraguchi, Masao Nagase, Takeshi Yasui, Yohsuke Kinouchi, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : 2021年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み, LED総合フォーラム2022 in 徳島, P-1, Jan. 2022.
5.	Retsuo Kawakami, Jin Ueda, 味元勇樹, Akihiro Shirai, Katsuyuki Miyawaki and 吉田雅彦 : プラズマ支援熱焼結処理したアナターゼTiO2ナノ粒子の光殺菌と鮮度保持効果, LED総合フォーラム2022 in 徳島, 133-134, Jan. 2022.
6.	Masanobu Haraguchi, Takahiro Kitada, Masao Nagase, Takeshi Yasui, Yohsuke Kinouchi, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : 2020年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み, LED総合フォーラム2021 in 徳島, P-1, Feb. 2021.
7.	Retsuo Kawakami, 味元勇樹, Katsuyuki Miyawaki, Akihiro Shirai and 吉田雅彦 : 熱支援プラズマ処理したアナターゼ/ルチル混晶型光触媒TiO2ナノ粒子の光分解と鮮度保持効果, LED総合フォーラム2021 in 徳島, 145-146, Feb. 2021.
8.	Retsuo Kawakami, 髙見直樹, 芳谷勇樹, 味元勇樹, 東知里, Katsuyuki Miyawaki, Akihiro Shirai, 吉田雅彦 and 山路諭 : 可視光LED照射下でのプラズマ支援アニーリングした光触媒TiO2ナノ粒子の鮮度保持効果, LED総合フォーラム2020 in 徳島, 83-84, Feb. 2020.
9.	Aya Nakai, koji Murai, Akihito Tanaka, Hiroshi Kondoh, Sugina Watanabe, Akiko Uebansou, Takahito Watanabe and Katsuyuki Miyawaki : LED植物工場を活用した藍の生育と二次代謝物質生産の制御, LED総合フォーラム2019 in徳島, 63-64, Feb. 2019.
10.	Masanobu Haraguchi, Takahiro Kitada, Masao Nagase, Takeshi Yasui, Yohsuke Kinouchi, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : LEDライフイノベーション総合プラットフォーム推進事業の取り組み, LED総合フォーラム2019in徳島, P-2, Feb. 2019.
11.	Retsuo Kawakami, Katsuyuki Miyawaki, Akihiro Shirai, 吉田雅彦, 芳谷勇樹, 髙見直樹, 東知里 and 山路諭 : 可視光LEDによる光触媒ナノ複合材シートの鮮度保持効果, LED総合フォーラム2019 in 徳島, 77-78, Feb. 2019.
12.	Katsuyuki Miyawaki, Yui Fukawa, Junichi Kawanabe, Takashi Yazumi, Yohei Matsuoka, Shuntaro Mataka and Shinichiro Isobe : 新規蛍光色素Fluolidを用いた蛍光in situ hybridization法の開発, 第41回日本分子生物学会年会, Nov. 2018.
13.	Takeshi Nikawa, Akira Takahashi, Katsuyuki Miyawaki and Yoshihiro Deguchi : 無重力や寝たきりによる筋萎縮の分子メカニズムとその栄養学的アプローチ, ナノオプティクス研究グループ第25回研究討論会, Nov. 2018.
14.	Masanobu Haraguchi, Takahiro Kitada, Masao Nagase, Takeshi Yasui, Yohsuke Kinouchi, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み, LED総合フォーラム2018in徳島, P-2, Feb. 2018.
15.	Retsuo Kawakami, Katsuyuki Miyawaki, Akihiro Shirai, 東知里, 芳谷勇樹, 吉岡誠人, 南雄也, 山路諭, 吉田雅彦, 大西和男 and 大野民之助 : LED照射下での光触媒ナノ複合材の鮮度保持機能, LED総合フォーラム2018 in 徳島, 125-126, Feb. 2018.
16.	Retsuo Kawakami, Katsuyuki Miyawaki, Akihiro Shirai, 東知里, 冨士本賢吾, 大塩誠二, 山路諭, 吉田雅彦, 大西和男, 大野民之助 and 松下俊雄 : LED光触媒ナノ粒子を使った鮮度保持技術の開発, LED総合フォーラム2016 in 徳島, 157-160, Dec. 2016.
17.	Masanobu Haraguchi, Yohsuke Kinouchi, Takahiro Kitada, Masao Nagase, Takeshi Yasui, Katsuyuki Miyawaki, Akira Takahashi and Toshiya Okahisa : LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み, LED総合フォーラム2016in徳島論文集, 201-202, Dec. 2016.
18.	Retsuo Kawakami, Katsuyuki Miyawaki, 玉谷元, 東知里, 宇野久史, 大塩誠二, 山路諭, 吉田雅彦, 大西和男, 大野民之助 and 松下俊雄 : LED光触媒に基づく鮮度保持技術のスケールアップ化, LED総合フォーラム2015 in 徳島, 117-120, Dec. 2015.
19.	Tsunedomi Akari, Takaaki Shimohata, Gotoh Marin, Amano Sachie, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari, Katsuyuki Miyawaki, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Campylobacter jejuniに対するUVA-LEDの有効性, 第8回日本カンピロバクター研究会総会, Dec. 2015.
20.	Retsuo Kawakami, Katsuyuki Miyawaki, 玉谷元, 榎木一史, 白濱達夫, 大塩誠二, 山路諭, 吉田雅彦 and 前田鎮廣 : 鮮度保持のためのLED光触媒技術に関する研究, LED総合フォーラム2014-2015 in 徳島, 137-140, Jan. 2015.

Patent:

1.	Sumihare Noji, 川那辺純一, Katsuyuki Miyawaki, 木田琢郎, 佐々木啓幸, 佐藤靖夫, 平田和弘 and 金慶日 : 3次元イムノクロマトグラフィー方式を用いた糖尿病検査診断用シート，糖尿病検査診断用デバイス，およびミオイノシトールの検出方法, 2013-239148 (Nov. 2013), 2015-099094 (May 2015), .
2.	Sumihare Noji, 川那辺純一, Katsuyuki Miyawaki, 木田琢郎, 佐々木啓幸, 佐藤靖夫, 平田和弘 and 金慶日 : 3次元イムノクロマトグラフィー方式を用いた検査診断用シート，検査診断用デバイス，および標識物の検出方法, 2013-239149 (Nov. 2013), 2015-099095 (May 2015), .
3.	Sumihare Noji, 金慶日, Katsuyuki Miyawaki, 佐々木啓幸, 平田和弘, 佐藤靖夫, 平石佳之 and 三ツ森正之 : 3次元検査診断用シート，3次元検査診断用デバイス，3次元検査診断用シートの製造方法および検査診断方法, P2012284231 (Dec. 2012), P2014126484A (Jul. 2014), 2012-284231 (Dec. 2012).

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

代謝の光制御によるタデアイ有用物質の増産技術の開発 (Project/Area Number: 24K08863 )
Studies on the electrostatic surface potential analysis of fungus spores for the improvement of photofungicidal technology by the combination with polyphenols (Project/Area Number: 20H02934 )
Examination of gem one editing for effective plant breeding (Project/Area Number: 25850004 )
Search by Researcher Number (80380111)

Associate Professor : Miyawaki, Katsuyuki

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)