徳島大学 / 教育研究者総覧 --- 中川弘

個人のホームページ

https://researchmap.jp/read0046140 (researchmap)

専門分野

○	障害者歯科学 (Disability and Oral Health), 小児歯科学 (Pediatric Dentistry)
○	神経解剖学 (Neuroanatomy)
○	神経解剖学 (Neuroanatomy)
○	神経科学 (Neuroscience)

研究テーマ

○	自閉症患者に対する視覚素材の有効性について (障害者歯科, 小児歯科)
○	三叉神経中のGABAの役割に関する研究，カプサイシンの作用に関する研究 (GABA (GABA), カプサイシン (capsaicin))
○	角膜に分布する神経の特性について (角膜, 感覚神経, カプサイシン, TRPチャネル)
○	TRPチャネルを用いた新規局所麻酔薬の開発 (TRPチャネル, 局所麻酔薬, カプサイシン (capsaicin), メンソール, QX-314)

著書・論文

学術論文(審査論文):

1.	Anrizandy Narwidina, Aya Miyazaki, Kokoro Iwata, Rika Kurogoushi, Asuna Sugimoto, Yasusei Kudo, Keita Kawarabayashi, Yoshihito Yamakawa, Yuki Akazawa, Takamasa Kitamura, Hiroshi Nakagawa, Kimiko Ueda Yamaguchi, Tomokazu Hasegawa, Keigo Yoshizaki, Satoshi Fukumoto, Akihito Yamamoto, Naozumi Ishimaru, Tomonori Iwasaki and Tsutomu Iwamoto : Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression., Biochemical and Biophysical Research Communications, Vol.650, 47-54, 2023. (要約) Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation. (キーワード) Homeodomain Proteins / Transcription Factors / Odontoblasts / Genes, Homeobox / 細胞分化 (cell differentiation) / 細胞増殖·分化 (cell proliferation and differentiation) (徳島大学機関リポジトリ) ● Metadata: 118052 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbrc.2023.02.004 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36773339 ● Search Scopus @ Elsevier (PMID): 36773339 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2023.02.004 (徳島大学機関リポジトリ: 118052, DOI: 10.1016/j.bbrc.2023.02.004, PubMed: 36773339)
2.	黒厚子璃佳, 岩田こころ, 中川弘, 長谷川智一, 上田(山口) 公子, 北村尚正, 赤澤友基, 杉本明日菜, 河原林啓太, 宮嵜彩, 尼寺理恵, 藤島史帆, 岩﨑智憲, 岩本勉 : 当科における5年間の障害児・有病児の初診時実態調査, 小児歯科学雑誌, Vol.60, No.1, 20-27, 2022年. (要約) 障害児や有病児への理解の深まりから社会環境や福祉制度の整備が進みつつあるが，障害や全身疾患をもつ児が地域において疾病予防も含めた歯科医療を受けられる環境がいまだ十分にあるとは言えず，実情に応じた環境整備が求められる．そこで，今回，平成25年度から平成29年度に当科を受診した児のうち障害児・有病児の5年間の初診時実態調査を行い，過去に当科で行った調査と比較し，近年の変化を検討した．初診患者1,301名のうち，障害児・有病児の割合は49 (キーワード) う蝕先天奇形口蓋裂口唇裂障害者歯科医療発達障害染色体異常小児歯科医療慢性疾患患者歯科医療病院歯科診療部門紹介と相談年齢因子徳島県実態調査地域差ヒト小児(6∼12) 男女歯学
3.	Rika Kurogoushi, Tomokazu Hasegawa, Yuki Akazawa, Kokoro Iwata, Asuna Sugimoto, Kimiko Ueda Yamaguchi, Aya Miyazaki, Anrizandy Narwidina, Keita Kawarabayashi, Takamasa Kitamura, Hiroshi Nakagawa, Tomonori Iwasaki and Tsutomu Iwamoto : Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells, Experimental and Therapeutic Medicine, Vol.22, No.6, 1356, 2021. (要約) The regulation of the mesenchymal stem cell (MSC) programming mechanism promises great success in regenerative medicine. Tissue regeneration has been associated not only with the differentiation of MSCs, but also with the microenvironment of the stem cell niche that involves various cytokines and immune cells in the tissue regeneration site. In the present study, fibroblast growth factor 2 (FGF2), the principal growth factor for tooth development, dental pulp homeostasis and dentin repair, was reported to affect the expression of cytokines in human dental pulp-derived MSCs. FGF2 significantly inhibited the expression of chemokine C-C motif ligand 11 (CCL11) in a time- and dose-dependent manner in the SDP11 human dental pulp-derived MSC line. This inhibition was diminished following treatment with the AZD4547 FGF receptor (FGFR) inhibitor, indicating that FGF2 negatively regulated the expression of CCL11 in SDP11 cells. Furthermore, FGF2 activated the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNK) in SDP11 cells. The mechanism of the FGFR-downstream signaling pathway was then studied using the SB203580, U0126 and SP600125 inhibitors for p38 MAPK, ERK1/2, and JNK, respectively. Interestingly, only treatment with SP600125 blocked the FGF2-mediated suppression of CCL11. The present results suggested that FGF2 regulated the expression of cytokines and suppressed the expression of CCL11 via the JNK signaling pathway in human dental pulp-derived MSCs. The present findings could provide important insights into the association of FGF2 and CCL11 in dental tissue regeneration therapy. (徳島大学機関リポジトリ) ● Metadata: 117039 (出版サイトへのリンク) ● Publication site (DOI): 10.3892/etm.2021.10791 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34659502 ● Search Scopus @ Elsevier (PMID): 34659502 ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2021.10791 (徳島大学機関リポジトリ: 117039, DOI: 10.3892/etm.2021.10791, PubMed: 34659502)
4.	岩田こころ, 黒厚子璃佳, 杉本明日菜, 藤島史帆, 赤澤友基, 河原林啓太, 宮嵜彩, 北村尚正, 尼寺理恵, 上田(山口) 公子, 中川弘, 長谷川智一, 岩本勉 : 当科における初診患者の実態調査∼平成における30年間の変遷∼, 小児歯科学雑誌, Vol.59, No.1, 8-13, 2021年. (要約) <p>2019年4月末日をもって平成時代が終わり，令和時代が幕を開けた．平成の約30年間は経済成長の低迷，晩婚化・少子化の加速，一方で高度化する情報通信技術等，小児を取り巻く環境は，昭和時代から急速に変化しさまざまな領域で二極化が顕在化してきた側面もある．われわれ小児歯科医は，社会環境・家庭環境などの変化が子供の発育に及ぼす影響や問題を的確に読み取り，対応と支援を考えなければならない．そこで，平成元年から平成30年を初期(平成元年度∼5年度)，中期(平成9年度∼13年度)，後期(平成25年度∼29年度)の各5年間で区切り，15年間の計5,982人を対象に当科に来院した初診患者の調査・分析を行った．</p><p>その結果，初診患者数は，年少人口の減少に伴い初期2,477人から後期1,301人と減少した．男女比は，各時期ともに概ね1:1であった．年齢は，いずれも学童期が約40%と最も多く，年齢構成に大きな変化はみられなかった．主訴は，各時期ともに齲蝕が最も多いが，初期の44%から後期には27%と減少した．紹介状を持参した児の割合は，初期の23%から後期は83.3%に増加し，障害児・有病児の割合が初期13.8%から後期33%に増加した．初期に比べ，主訴は多様化し，かかりつけ歯科医院と大学病院の機能分化が進んでいることが明らかとなった．今後は，さらに医科歯科連携の重要性が高まることが示唆された．</p> (キーワード) 実態調査 / 初診患者 / 平成 / 小児歯科 / Survey of actual state / New patients / Heisei / Pediatric dentistry (出版サイトへのリンク) ● Publication site (DOI): 10.11411/jspd.59.1_8 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390854139452228096 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.59.1_8 (DOI: 10.11411/jspd.59.1_8, CiNii: 1390854139452228096)
5.	Kimiko Ueda Yamaguchi, Yuki Akazawa, Kawarabayashi Keita, Asuna Sugimoto, Hiroshi Nakagawa, miyazaki aya, Weih Falk, Kurogoushi Rika, Iwata Kokoro, Takamasa Kitamura, Yamada Aya, Tomokazu Hasegawa, Fukumoto Satoshi and Tsutomu Iwamoto : Combination of ions promotes cell migration via extracellular signalregulated kinase 1/2 signaling pathway in human gingival fibroblasts., Molecular Medicine Reports, Vol.19, No.6, 5039-5045, 2019. (要約) Wound healing is a dynamic process that involves highly coordinated cellular events, including proliferation and migration. Oral gingival fibroblasts serve a central role in maintaining oral mucosa homeostasis, and their functions include the coordination of physiological tissue repair. Recently, surface pre-reacted glass-ionomer (S-PRG) fillers have been widely applied in the field of dental materials for the prevention of dental caries, due to an excellent ability to release fluoride (F). In addition to F, S-PRG fillers are known to release several types of ions, including aluminum (Al), boron (B), sodium (Na), silicon (Si) and strontium (Sr). However, the influence of these ions on gingival fibroblasts remains unknown. The aim of the present study was to examine the effect of various concentrations of an S-PRG filler eluate on the growth and migration of gingival fibroblasts. The human gingival fibroblast cell line HGF-1 was treated with various dilutions of an eluent solution of S-PRG, which contained 32.0 ppm Al, 1,488.6 ppm B, 505.0 ppm Na, 12.9 ppm Si, 156.5 ppm Sr and 136.5 ppm F. Treatment with eluate at a dilution of 1:10,000 was observed to significantly promote the migration of HGF-1 cells. In addition, the current study evaluated the mechanism underlying the mediated cell migration by the S-PRG solution and revealed that it activated the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), but not of p38. Furthermore, treatment with a MEK inhibitor blocked the cell migration induced by the solution. Taken together, these results suggest that S-PRG fillers can stimulate HGF-1 cell migration via the ERK1/2 signaling pathway, indicating that a dental material containing this type of filler is useful for oral mucosa homeostasis and wound healing. (徳島大学機関リポジトリ) ● Metadata: 113289 (出版サイトへのリンク) ● Publication site (DOI): 10.3892/mmr.2019.10141 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31059063 ● Summary page in Scopus @ Elsevier: 2-s2.0-85066160258 (徳島大学機関リポジトリ: 113289, DOI: 10.3892/mmr.2019.10141, PubMed: 31059063, Elsevier: Scopus)
6.	上田(山口) 公子, 郡由紀子, 中川弘, 岩本勉 : 自閉スペクトラム症児者の特性と歯科受診時の適応状態および唾液α-アミラーゼ活性値との関連について, 障害者歯科, Vol.37, No.4, 401-406, 2016年. (出版サイトへのリンク) ● Publication site (DOI): 10.14958/jjsdh.37.401 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390282679459461632 ● Search Scopus @ Elsevier (DOI): 10.14958/jjsdh.37.401 (DOI: 10.14958/jjsdh.37.401, CiNii: 1390282679459461632)
7.	郡由紀子, 上田(山口) 公子, 中川弘, 岩本勉 : 広汎性発達障害児者の歯科受診適応性評価の試み, 障害者歯科, Vol.37, No.2, 186-191, 2016年. (出版サイトへのリンク) ● Publication site (DOI): 10.14958/jjsdh.37.186 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390001204484694400 ● Search Scopus @ Elsevier (DOI): 10.14958/jjsdh.37.186 (DOI: 10.14958/jjsdh.37.186, CiNii: 1390001204484694400)
8.	山本愛美, 中川弘, 郡由紀子, 北村尚正, 杉本明日菜, 岩本勉 : 災害時要援護者である小児および障碍児・者を持つ保護者の防災意識に関する調査, 小児歯科学雑誌, Vol.53, No.3, 373-382, 2015年. (キーワード) 災害医療 / 災害時要援護者 / 歯科医療支援 / 防災意識 (出版サイトへのリンク) ● Publication site (DOI): 10.11411/jspd.53.3_373 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390001205519929088 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.53.3_373 (DOI: 10.11411/jspd.53.3_373, CiNii: 1390001205519929088)
9.	Yuki Akazawa, Tomokazu Hasegawa, Yoshitaka Yoshimura, Naoyuki Chosa, Takeyoshi Asakawa, Kimiko Ueda Yamaguchi, Asuna Sugimoto, Takamasa Kitamura, Hiroshi Nakagawa, Akira Ishisaki and Tsutomu Iwamoto : Recruitment of mesenchymal stem cells by stromal cell-derived factor 1 in pulp cells from deciduous teeth, International Journal of Molecular Medicine, Vol.36, 442-448, 2015. (要約) Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. Therefore, the aim of the present in vitro study was to investigate whether stromal cell-derived factor 1 (SDF1)-C-X-C chemokine receptor type 4 (CXCR4) signaling is involved in tissue repair in the DP of deciduous teeth. A single-cell clone from DPCs (SDP11) and UE7T-13 cells were used as pulp cells and MSCs, respectively. The MG-63 and HuO9 cells, two osteosarcoma cell lines, were used as positive control cells. Reverse transcription polymerase chain reaction (RT-PCR) revealed that all cell lines (SDP11, UE7T-13 MG-63 and HuO9) were positive for both SDF1 and CXCR4 mRNA expression. Moreover, immunocytochemical analysis indicated that SDF1 and CXCR4 proteins were expressed in the SDP11 and UE7T-13 cells. SDF1 was also detected in the cell lysates (CLs) and conditioned medium (CM) collected from the SDP11 and UE7T-13 cells, and AMD3100, a specific antagonist of CXCR4, inhibited the migration of the UE7T-13 cells; this migration was induced by treatment with CM, which was collected from the SDP11 cells. In addition, real-time PCR showed that the expression of SDF1 in the SDP11 cells was inhibited by treatment with 20 ng/ml fibroblast growth factor (FGF)-2, and exposure to AZD4547, an inhibitor of the FGF receptor, blocked this inhibition. Collectively, these data suggest that SDF1 produced by DP plays an important role in homeostasis, repair and regeneration via the recruitment of MSCs. (出版サイトへのリンク) ● Publication site (DOI): 10.3892/ijmm.2015.2247 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26082290 ● Summary page in Scopus @ Elsevier: 2-s2.0-84934271560 (DOI: 10.3892/ijmm.2015.2247, PubMed: 26082290, Elsevier: Scopus)
10.	Hiroshi Nakagawa and Akio Hiura : QX-314 Induces Analgesia to Nociceptive Thermal Stimulus by Co-Application with Capsiate or Anandamide, Austin Biomarkers & Diagnosis, Vol.1, No.1, 1-4, 2014.
11.	桃田幸弘, 高野栄之, 可児耕一, 高石和美, 中川弘, 富岡重正, 郡由紀子, 橋本俊顕, 北畑洋, 東雅之 : いわゆる病病連携による障害者歯科医療連携が奏功した集学的歯科治療について, 四国歯学会雑誌, Vol.26, No.2, 35-40, 2014年.
12.	Yukiko Kori, Kimiko Ueda Yamaguchi, Aimi Yamamoto, Hiroshi Nakagawa, Masami Yoshioka and Tsutomu Iwamoto : Evaluation of dental treatment adaptability for patient with pervasive developmental disorder(PDD), Journal of Disability and Oral Health, Vol.15, No.3, 73, 2014.
13.	長谷川智一, 赤澤友基, 永井宏和, 北村尚正, 石丸直澄, 上田(山口) 公子, 中川弘, 郡由紀子, 山本愛美, 岩本勉 : 幼児期の小児の口蓋に発生した血管腫の1例, 小児歯科学雑誌, Vol.52, No.3, 448-453, 2014年. (要約) 血管腫は血管の増殖を特徴とする良性腫瘍であり，小児の顎口腔領域における良性腫瘍のなかでは比較的多く，軟組織腫瘍の中で最も多い．顎口腔領域に発生する本腫瘍は舌，口唇，歯肉，頬粘膜に好発し，口蓋に発生することは稀である．今回，われわれは，2 歳10 か月男児の口蓋に発生した血管腫を経験したので，その概要を報告する．患児は初診の約4 か月前に下顎部を打撲したため近医歯科を受診した．その際，上顎両側乳中切歯口蓋側歯肉の腫瘤を指摘され，単純性歯肉炎の診断下にプラークコントロールを受けていたが改善しないため当科を紹介された．初診時，切歯乳頭から上顎両側乳中切歯口蓋側歯肉にかけて大きさ10×8×5mm類円形で境界明瞭，暗赤色，表面不整，弾性軟の腫瘤を認め，上顎腫瘍と診断した．局所麻酔下に腫瘍切除術を施行した．病理組織学的診断は毛細血管腫であった．今後，再発に注意しながら，少なくとも永久歯交換まで厳重な経過観察が必要と考えられた． (キーワード) 血管腫 / 口蓋 / 幼児期 (出版サイトへのリンク) ● Publication site (DOI): 10.11411/jspd.52.3_448 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390282680495239168 ● Search Scopus @ Elsevier (DOI): 10.11411/jspd.52.3_448 (DOI: 10.11411/jspd.52.3_448, CiNii: 1390282680495239168)
14.	Hiroshi Nakagawa and Akio Hiura : Comparison of the transport of QX-314 through TRPA1, TRPM8 and TRPV1 channels (Cited in "Current Neuropharmacology, 12/2013 11(6); Pharmacological Review, 2014 66(3)"), Journal of Pain Research, Vol.6, 223-230, 2013. (要約) It has been demonstrated that N-ethyl-lidocaine (QX-314) can target the transient receptor protein vanilloid 1 (TRPV1) nociceptors when coadministered with capsaicin, resulting in a selective block of the nociceptors. Capsaicin is problematic in therapeutic use because it induces firing of nociceptors. The present study aimed to search for substitutes for capsaicin. We also examined the transportability of QX-314 into nociceptive neurons, through the pores of transient receptor potential ankyrin 1 (TRPA1), transient receptor potential melastatin-8 (TRPM8), and TRPV1. To investigate the effect on TRPA1, injections of a vehicle, allyl isothiocyanate (AITC), QX-314, or AITC/QX-314 were made into the hind paws of rats. The effects of menthol and capsaicin on the opening of TRPM8 and TRPV1 were also examined and compared with the potency of QX-314. To examine inhibition of the antinociceptive effect by capsaicin/ QX-314, capsazepine (50 g/mL; 10 L) was injected 30 minutes prior to capsaicin/QX-314 (10 L) injection. Thermal sensitivity was investigated by the Hargreaves method. 5(6)-carboxyfluorescein (FAM)-conjugated QX-314 was used as a tracer to examine how many and which kind of dorsal root ganglia accumulate this molecule. QX-314-FAM, capsaicin/QX-314-FAM, AITC/QX-314-FAM, and menthol/QX-314-FAM were injected into the paw. Two weeks after injections, dorsal root ganglia were removed and sectioned with a cryostat. The capsaicin/QX-314 group induced longer withdrawal-response latency at 60 to 300 minutes after injection than the control. Both menthol only and menthol/QX-314 injections showed analgesia 10 to 60 minutes after injection. No significant difference was seen between the capsazepine/capsaicin/QX-314 group and the vehicle group. The fluorescence in small- and medium-sized neurons was conspicuous in only the dorsal root ganglia injected with capsaicin/ QX-314-FAM. These results indicate that TRPA1 and TRPM8 are ineffective in the transport of QX-314 compared with TRPV1. (出版サイトへのリンク) ● Publication site (DOI): 10.2147/JPR.S41614 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23525210 ● Search Scopus @ Elsevier (PMID): 23525210 ● Search Scopus @ Elsevier (DOI): 10.2147/JPR.S41614 (DOI: 10.2147/JPR.S41614, PubMed: 23525210)
15.	山本愛美, 中川弘 : 自閉症患者へのブラッシング指導における視覚支援の効果と継続的支援による学習効果, 障害者歯科, Vol.34, No.1, 12-18, 2013年. (キーワード) 自閉症 (autism) / Tooth-brushing instruction / Visual support / Continuous support (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1570291226221013504 (CiNii: 1570291226221013504)
16.	Akio Hiura and Hiroshi Nakagawa : Changes in response behaviors to noxious heat and mechanical stimuli after carrageenan-induced inflammation in mice treated with capsaicin 2 or 15 days after birth, WebmedCentral NEUROSCIENCES, 1-13, 2012. (要約) The aim of the present study was to investigate thecause of normal response behaviors to noxious heatdespite the marked loss of small neurons in the dorsalroot ganglion (DRG) as a result of neonatal capsaicintreatment. Capsaicin (50 mg/kg) was injectedsubcutaneously (s.c.) into mice on either day P2 orP15; control mice received a vehicle injection. Twentydays after the capsaicin injection, 2% carrageenan (20μL) was injected into the right hind paw of each animal.Twenty-four hours after the carrageenan injection,behavioral tests using noxious heat stimuli (NHS;Hargreaves method) and noxious mechanical stimuli(NMS; von Frey method) were performed using thecontrol and capsaicin-treated mice. Pre-carrageenanmeasurements were used as the baseline values foreach group. After the experiments, the mice wereperfused with a mixture of 4% paraformaldehyde and0.2% picric acid in a 0.1-M phosphate buffer (pH 7.4).The L4 and L5 DRGs were extracted, sectioned usinga cryostat, and immunostained using a TRPV1antibody and reacted with isolectin IB4. The P2capsaicin-injected mice exhibited a marked increase intheir analgesic responses to NHS, compared with boththeir baseline values and the respective control mice.Both the capsaicin-treated and control animalsexhibited significant hyperalgesia in response to NMS.Naturally, the control P15 mice exhibited a shorterresponse time to NHS than their baseline values, whilethe capsaicin-injected P15 mice exhibited increasedalgesia comparable to the baseline values of thecontrol. The capsaicin-injected mice also exhibitedalgesia in response to NMS. The number ofTRPV1-immunoreactive (ir) small neurons decreasedby 90% in the P2 capsaicin-injected mice and 45% inthe P15 capsaicin-injected mice, whereas an increasein IB4-positive neurons was seen in both the P2 andP15 capsaicin-injected mice. In association with thedecrease in larger neurons, the numbers of smallerneurons were increased in both P2 and P15capsaicin-injected mice. TRPV1-ir small neurons areclosely correlated with inflammatory heat painperception, suggesting the enhancement of anunknown acute noxious heat sensor in the presentstudy. The present findings indicate that noxiousmechanical stimuli can be sensed despite thepresence or absence of TRPV1-ir or IB4-positiveneurons. (徳島大学機関リポジトリ) ● Metadata: 104177 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1050001337734605568 (徳島大学機関リポジトリ: 104177, CiNii: 1050001337734605568)
17.	Akio Hiura and Hiroshi Nakagawa : Analysis of DNA extracted from the trigeminal ganglion cells after neonatal capsaicin treatment by agarose gel electrophoresis, WebmedCentral TOXICOLOGY, Vol.3, No.9: WMC003708, 1-6, 2012. (徳島大学機関リポジトリ) ● Metadata: 103612 (徳島大学機関リポジトリ: 103612)
18.	Akio Hiura and Hiroshi Nakagawa : Innervation of TRPV1-, PGP-, and CGRP-immunoreactive nerve fibers in the subepithelial layer of the whole mount preparation of the rat cornea(Cited in "Survey of Opthalmology, 2014; Journal of Chemical Neuroanatomy, 01/2014"), Okajimas Folia Anatomica Japonica, Vol.89, No.2, 47-50, 2012. (要約) The pattern of innervation of capsaicin receptor, TRPV1-(transient receptor protein vanilloid 1), PGP 9.5-(protein gene product, a marker of peripheral nerve fibers)-, and CGRP (calcitonin gene-related peptide)-immunoreactive (IR) nerve fibers was examined by immunohistological staining of whole mount preparations of the adult rat cornea. The outer corneoscleral limbus toward the central cornea in the subepithelial (stromal) layer was richly innervated by a meshwork of PGP- and CGRP-IR nerve fibers. Sparse innervation was observed in the central cornea, presumably owing to insufficient staining. Dense innervation of TRPV1-IR nerve fibers were demonstrated in addition to innervation of PGP- and CGRP-IR nerve fibers, running from the corneoscleral margin to the central cornea. Although the density of TRPV1-IR nerve fibers appeared to gradually diminish, immunostaining of TRPV1-IR nerve fibers was not as clear as that of PGP- and CGRP-IR nerve fibers. The TRPV1-IR nerve fibers appeared to be thinner than the PGP- and CGRP-IR nerve fibers. The TRPV1-IR leash fibers were observed in the basal epithelial layer, presumably ensuring effective corneal reflex, response to noxious stimuli, and repair of cornea injury. (キーワード) Animals / Biological Markers / Calcitonin Gene-Related Peptide / Epithelium, Corneal / Male / Nerve Fibers / Rats / TRPV Cation Channels / Ubiquitin Thiolesterase (出版サイトへのリンク) ● Publication site (DOI): 10.2535/ofaj.89.47 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23117304 ● Search Scopus @ Elsevier (PMID): 23117304 ● Search Scopus @ Elsevier (DOI): 10.2535/ofaj.89.47 (DOI: 10.2535/ofaj.89.47, PubMed: 23117304)
19.	Hiroshi Nakagawa, Akio Hiura, Masato Mitome and Kazunori Ishimura : Nerve fibers that were not stained with the non-specific acetylcholinesterase (NsAchE) method, and TRPV1- and IB4-positive nerve fibers in the rat cornea.(Cited in "Journal of Chemical Neuroanatomy, 01/2014"), The Journal of Medical Investigation : JMI, Vol.56, No.3,4, 157-165, 2009. (要約) Previously, we noticed the presence of nerve fiber-like structures in a whole mount preparation of the rat cornea that had not been stained with the non-specific acetylcholinesterase (NsAchE) method. These nerve-like fibers were projected into the central area of the cornea, forming a mesh-like pattern. The aim of this study is to examine the properties of these mesh-like fibers using the following two methods: their sensitivity to capsaicin and the detection of isolectin B4 (IB4)- and capsaicin receptor TRPV1 (transient receptor potential vanilloid 1)-reactivities. The mean disappeared area of non-stained fibers after NsAchE treatment was 26% of the total areas in the neonatally capsaicin-treated cornea. Bunches composed of fine IB4-positive nerve fibers were seen in a whole mount preparation. There were connections between the bunches, producing a mesh-like pattern similar to that of the fibers that were not stained with NsAchE. Fine TRPV1-immunoreactive (ir) nerve fibers were also shown to form bunches, with connections between each bunch observed in whole mount preparations. Thus, TRPV1-ir nerve fibers seem to densely innervate the rat corneal subepithelial stroma and are distinct from the NsAchE-positive nerve fibers. The TRPV1-ir fine nerve fibers overlapped with the IB4-positive nerve fibers, suggesting that the mesh-like fibers that were not stained with NsAchE are fine nociceptive sensory nerve fibers because of their sensitivity to capsaicin and similar distribution pattern to IB4- and TRPV1-positive nerve fibers. (キーワード) TRPV1 / IB4 / Nociceptive sensory nerve fibers / 角膜 (cornea) / カプサイシン (capsaicin) (徳島大学機関リポジトリ) ● Metadata: 111323 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.56.157 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19763029 ● Search Scopus @ Elsevier (PMID): 19763029 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.157 (徳島大学機関リポジトリ: 111323, DOI: 10.2152/jmi.56.157, PubMed: 19763029)
20.	Akio Hiura and Hiroshi Nakagawa : Induction of corneal lesion and nerve fiber sprouting by neonatal capsaicin application depends on the dose of the drug and survival time after treatment, Okajimas Folia Anatomica Japonica, Vol.82, No.2, 57-66, 2005. (要約) The effects of capsaicin on the rat cornea and its NsAchE (non specific acetylcholinesterase)-positive nerve fibers were investigated after long and short survival periods following subcutaneous (s.c) injection of the drug. Sixteen rats were injected once s.c with capsaicin on postnatal day 2 at a dose of 50 mg/kg. Age-matched rats were injected only vehicle, and served as control (n = 16). After 4 (n = 6), 8 (n = 6) and 12 (n = 4) mos., both sides of the corneas were examined under a binocular microscope to look for corneal abnormalities. Immediately after the enucleation, bilateral corneas were excised with a thin scleral margin and their ciliary body and iris were removed in DPBS solution. Then, they were fixed in a 4% paraformaldehyde solution containing 8% sucrose for 1 h at 4 degrees C, and processed for staining by the NsAchE method. For comparison, 15 rats were injected 3 times with capsaicin (total dose: 150 mg/kg) at a dose of 50 mg/kg on days 1, 2 and 3 after birth. Age-matched rats (n = 16) were injected vehicle as controls. Five (n = 4), 17 (n = 4), 45 (n = 3) and 75 (n = 4) days later, their corneas were similarly handled as described above. Corneal lesions and sprouting of the NsAchE-positive subepithelial nerve fibers appeared 4 mos. after the treatment with capsaicin (50 mg/kg). In particular, all the treated corneas (8/8) at 12 mos. showed corneal abnormalities. Contrary to the results from the single injection of capsaicin, corneal changes had already appeared on day 17 after treatment with capsaicin at a high dose (150 mg/kg). These data reinforce the suggestion that yielding of corneal lesions is closely associated with the longer survival time at lower doses and with the short survival time at a high dose of the drug. Further, the relationship between sprouting of corneal nerve fibers and corneal wounds was discussed on the basis of the previous reports. (キーワード) Animals / Animals, Newborn / Capsaicin / Cornea / Dose-Response Relationship, Drug / Injections, Subcutaneous / Nerve Fibers / Rats (出版サイトへのリンク) ● Publication site (DOI): 10.2535/ofaj.82.57 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16212277 ● Summary page in Scopus @ Elsevier: 2-s2.0-27144543627 (DOI: 10.2535/ofaj.82.57, PubMed: 16212277, Elsevier: Scopus)
21.	Akio Hiura and Hiroshi Nakagawa : Capsaicin-resistant, nonspecific acetylcholinesterase (NsAchE) reactive nerve fibers in the rat cornea: a quantitative and developmental study (Cited in "Investigative Ophtalmology & Visual Science, 52(5), 2010"), Okajimas Folia Anatomica Japonica, Vol.81, No.4, 75-84, 2004. (要約) Little is known whether capsaicin affects on the nonspecific acetylcholinesterase (NsAchE) positive nerves in the cornea. To examine this point, the density of the corneal nerve fibers stained with NsAchE method was compared with that of control. Capsaicin (50 mg/kg) was injected once into the dorsal skin of rats at 2 day after birth. As controls, a vehicle was similarly injected into animals of the same age. At 5 (n = 3), 17 (n = 4), 45 (n = 4) and 75 (n = 3) days after treatment, the bilateral corneas were excised from capsaicin treated (n = 14) and age-matched control animals (n = 14). The entire subepithelial corneal nerves stained in whole mount preparations were hand traced. Their density was represented as mm/mm2 by converting the total areas of the nerve fibers into the total length of them using computer image analysis system. No significant differences in the nerve fiber density were seen in the control and capsaicin-treated corneas at the survival times examined. Even high doses of neonatal capsaicin injection (total: 150 mg/kg) no NsAchE-reacted corneal nerves showed a significant decrease. However, the time of wiping by instillation of 100 microM capsaicin onto the corneas severely reduced (analgesia to chemical irritant) in the neonatal capsaicin-treated rats (50 mg/kg, n = 13) compared with those of controls (n = 13) at 17, 45 and 75 day survivals. This was closely associated with the decrease of PGP-ir intraepithelial nerve fibers. The corneal nerves reacted with the NsAchE seemed to be capsaicin resistant and have a functional property except the chemical nociception. (キーワード) Acetylcholinesterase / Animals / Capsaicin / Cornea / Female / Male / Nerve Fibers / Rats / Rats, Wistar (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15615030 ● CiNii @ 国立情報学研究所 (CRID): 1390001206322613888 ● Search Scopus @ Elsevier (PMID): 15615030 (PubMed: 15615030, CiNii: 1390001206322613888)
22.	Hiroshi Nakagawa, Akio Hiura and YOSHIHIRO KUBO : Preliminary Studies on GABA-immunoreactive Neurons in the Rat Trigeminal Ganglion (Cited in "Cephalagia, 26(1), 2006; Molecular Pain, 09/2009; Neurochemical Journal, 8(2), 2014"), Okajimas Folia Anatomica Japonica, Vol.80, No.1, 15-22, 2003. (要約) GABA-immunoreactive (ir) primary sensory neurons have been reported in many studies. However, the role of GABA in the primary sensory neurons and their targets is quite open to question. The present study aimed to examine the GABA-ir neurons in the rat trigeminal ganglion (TG), for the first step of functional study on them. Some neurons in the TG showed GABA-ir, which were ranged from large to small size. The total number of examined TG neurons from 6 ganglia was 2,531, of which 462 neurons (18.3%) showed GABA-ir the large subpopulation of GABA-ir TG neurons is likely to involve in nerve-muscle functions, whereas medium and small subpopulations might participate in cutaneous nociceptive sensory function. The present findings demonstrated a considerable number of sensory neurons containing GABA in the rat TG. Functional studies on GABA-ir neurons in the TG would be an interesting and important issues in future studies. The next aim of our study is to examine the size distribution of GABAergic neurons and the coexistence with other neurotransmitters in the rat TG. (キーワード) GABA / Immunocytochemistry / Trigeminal ganglion / Primary sensory (出版サイトへのリンク) ● Publication site (DOI): 10.2535/ofaj.80.15 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12858961 ● Summary page in Scopus @ Elsevier: 2-s2.0-0038108719 (DOI: 10.2535/ofaj.80.15, PubMed: 12858961, Elsevier: Scopus)
23.	中川弘, 松本文博, 久保吉廣 : 成人型異染性白質ジストロフィーの1同胞例の歯科治療経験, 日本障害者歯科学会雑誌, Vol.24, No.1, 14-18, 2003年. (キーワード) Metachromatic leukodystrophy / Dental treatment / Swallowing disorders (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1570009749889129472 (CiNii: 1570009749889129472)
24.	Akio Hiura, Yoshiko Nakae and Hiroshi Nakagawa : Cell death of primary afferent nerve cells in neonatal mice treated with capsaicin (Cited in "European Journal of Pharmacology, 500(1-3), 2004; The book [Neurogenic Inflammation in Health and Disease] ed. by G. Jancso, Elsevier, 2009; Biomed Research International, 01/2014; 05/2014"), Anatomical Science International, Vol.77, No.1, 47-50, 2002. (要約) Capsaicin (50 mg kg-1) or vehicle (control) was injected into 2-day-old mice, and apoptotic cell death of the lumbar dorsal root ganglion (DRG) cells was examined using in situ terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method at 5, 24, 48 and 72 h after treatment. Apoptotic cells were counted per section under a fluorescent microscope. The mean number of apoptotic cells showed a significant increase in the capsaicin-treated DRG at 24 h, but the number was at most 1-2 per section. Thus, many previous findings of an approximate 70-80% loss of the small primary afferent neurons induced by capsaicin could not be explained by apoptosis alone. Moreover, according to the morphological changes, it appeared that capsaicin preferentially induced necrosis rather than apoptosis, at least in mice. (キーワード) アポトーシス (apoptosis) / カプサイシン (capsaicin) / necrosis / primary afferent neuron / TUNEL method (出版サイトへのリンク) ● Publication site (DOI): 10.1046/j.0022-7722.2002.00004.x (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12418083 ● Summary page in Scopus @ Elsevier: 2-s2.0-0036517299 (DOI: 10.1046/j.0022-7722.2002.00004.x, PubMed: 12418083, Elsevier: Scopus)
25.	中川弘, 樋浦明夫, 久保吉廣 : 新生仔期にカプサイシン投与したマウスの侵害熱刺激応答に対する抗NGFとNGFの影響, 日本薬理学雑誌, Vol.116, No.2, 93-100, 2000年. (キーワード) カプサイシン / 発芽 / 侵害熱刺激 / 抗NGF / NGF
26.	松本文博, 中川弘, 久保吉廣, 坂東永一 : 徳島大学歯学部附属病院特殊歯科総合治療部顎口腔機能治療部門における顎関節症の実態調査, 四国歯学会雑誌, Vol.12, No.2, 227-283, 2000年. (キーワード) 顎関節症 / 実態調査 / 臨床統計 / 症型分類
27.	Akio Hiura, Hiroshi Nakagawa, YOSHIHIRO KUBO, Yuichiro Koshigae, Ayumi Yoshizako and Hiroshi Ishizuka : Age-related changes in the response to thermal noxious heat and reduction of C-fibers by neonatal treatment with capsaicin (Cited in "Journal of Physiology, 549(1), 2003; International Review of Neurobiology, 87, 2009"), Somatosens Mot Res, Vol.16, No.2, 115-121, 1999. (キーワード) withdrawal response / nociceptive heat / C-fiber / capsaicin / neonatal age / mouse
28.	中川弘, 松本文博, 久保吉廣, 坂東永一 : 特殊歯科総合治療部を受診した患者の実態調査, 四国歯学会雑誌, Vol.11, No.2, 245-252, 1999年. (キーワード) 特殊歯科 / 障害者歯科 / 全身疾患有病者歯科 / 顎口腔機能治療 / 患者実態調査
29.	清水謙, 笠井啓次, 中川弘, 天野智子, 有田憲司, 西野瑞穗 : 小手術時の心身の状態把握のための看護アセスメント, 小児歯科学雑誌, Vol.36, No.3, 446-452, 1998年.
30.	中川弘, 石塚寛, 樋浦明夫 : マウスの腰部後根神経節と後根内有髄神経の生後発達, 醫學と生物學, Vol.136, No.5, 129-134, 1998年.
31.	中川弘, 原田桂子, 鎌田浩二, 宮本幸子, 有田憲司, 西野瑞穗 : 小児の歯科診療時の協力性に関する研究, --- 第5報母親の不安度と小児の歯科治療に対する適応性との関連 ---, 小児歯科学雑誌, Vol.29, No.2, 325-329, 1991年.
32.	中川弘, 原田桂子, 鎌田浩二, 宮本幸子, 有田憲司, 西野瑞穗 : 小児の歯科診療時の協力性に関する研究, --- 第4報小児の歯科治療前および治療中の身体行動および情動反応と治療に対する適応性との関連 ---, 小児歯科学雑誌, Vol.28, No.4, 984-995, 1990年.
33.	佐々木保行, 西野瑞穗, 鈴木敏昭, 有田憲司, 高梨一彦, 原田桂子, 岡本多恵, 中川弘 : 小児の歯科診療における適応性の心理学的研究(第2報), 鳴門教育大学研究紀要(教育科学編), Vol.3, 105-116, 1988年. (キーワード) 小児歯科診療 / 適応性 / 認知特性 / 人格テスト / 社会性発達
34.	西野瑞穗, 有田憲司, 原田桂子, 岡本多恵, 中川弘, グァダルーペアルバラードラリナガ, 佐々木保行, 鈴木敏昭 : 小児の歯科診療時の協力性に関する研究第1報, --- 歯科受診時の小児の行動と情緒安定度 ---, 小児歯科学雑誌, Vol.25, No.1, 100-108, 1987年.
35.	原田桂子, 西野瑞穗, 有田憲司, 岡本多恵, 中川弘, 佐々木保行, 鈴木敏昭 : 小児の歯科診療時の協力性に関する研究, --- 第3報小児の人格的要因と歯科受診時の行動との相関性 ---, 小児歯科学雑誌, Vol.25, No.4, 830-839, 1987年. (キーワード) 小児の行動と心理 / 幼児社会性発達検査 / マッチングテスト / 歯科診療意識調査

総説・解説:

1.	高石和美, 藤原茂樹, 江口覚, 大塚良, 赤澤友基, 北村尚正, 上田(山口) 公子, 中川弘, 可児耕一, 青田桂子, 桃田幸弘, 山村佳子, 工藤景子, 大江剛, 玉谷哲也, 宮本洋二, 東雅之, 岩本勉, 北畑洋 : 当院における全身麻酔下歯科治療について -障害をもつ方へ安全な歯科治療の提供-, Journal of Oral Health and Biosciences, Vol.31, No.1, 62-67, 2018年7月. (要約) We report about general anesthesia for disabled patients with special needs or young children during dental treatment in Tokushima University Hospital. We have administered ambulatory anesthesia or general anesthesia with short-term hospitalization for the patients. In this report, we showed anesthesia as a behavior management technique and the system for the patients who schedule ambulatory anesthesia in our hospital. We indicated the present situation of dental treatment under general anesthesia and supposed the prospects of dental care for the patients with special needs. (キーワード) 全身麻酔 / 障害者 / 日帰り全身麻酔 / 歯科治療 (徳島大学機関リポジトリ) ● Metadata: 111940 (出版サイトへのリンク) ● Publication site (DOI): 10.20738/johb.31.1_62 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390845712973457920 ● Search Scopus @ Elsevier (DOI): 10.20738/johb.31.1_62 (徳島大学機関リポジトリ: 111940, DOI: 10.20738/johb.31.1_62, CiNii: 1390845712973457920)
2.	Hiroshi Eguchi, Akio Hiura, Hiroshi Nakagawa, Kusaka Shunji and Shimomura Yoshikazu : Corneal nerve fiber structure, its role in corneal function, and its changes in corneal diseases, BioMed Research International, Vol.2017, 3242649, Nov. 2017. (要約) Recently, in vivo confocal microscopy is used to examine the human corneal nerve fibers morphology. Corneal nerve fiber architecture and its role are studied in healthy and pathological conditions. Corneal nerves of rats were studied by nonspecific acetylcholinesterase (NsAchE) staining. NsAchE-positive subepithelial (stromal) nerve fiber has been found to be insensitive to capsaicin. Besides, NsAchE-negative but capsaicin-sensitive subbasal nerve (leash) fibers formed thick mesh-like structure showing close interconnections and exhibit both isolectin B4- and transient receptor potential vanilloid channel 1- (TRPV1-) positive. TRPV1, TRPV3, TRPA (ankyrin) 1, and TRPM (melastatin) 8 are expressed in corneal nerve fibers. Besides the corneal nerve fibers, the expressions of TRPV (1, 3, and 4), TRPC (canonical) 4, and TRPM8 are demonstrated in the corneal epithelial cell membrane. The realization of the importance of TRP channels acting as polymodal sensors of environmental stresses has identified potential drug targets for corneal disease. The pathophysiological conditions of corneal diseases are associated with disruption of normal tissue innervation, especially capsaicin-sensitive small sensory nerve fibers. The relationships between subbasal corneal nerve fiber morphology and neurotrophic keratopathy in corneal diseases are well studied. The recommended treatment for neurotrophic keratopathy is administration of preservative free eye drops. (徳島大学機関リポジトリ) ● Metadata: 112390 (出版サイトへのリンク) ● Publication site (DOI): 10.1155/2017/3242649 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29238714 ● Summary page in Scopus @ Elsevier: 2-s2.0-85042164473 (徳島大学機関リポジトリ: 112390, DOI: 10.1155/2017/3242649, PubMed: 29238714, Elsevier: Scopus)
3.	Hiroshi Nakagawa : Current Status of Care for Patients with Disabilities in Tokushima Prefecture and Future Perspectives, Journal of Oral Health and Biosciences, Vol.30, No.1, 40-44, Jun. 2017. (キーワード) current status of dental care for patients with disabilities in Tokushima Prefecture / specialists of care for patients with disabilities / community liaison networks / dental approaches for patients with dementia
4.	Hiroshi Nakagawa and Akio Hiura : Four Possible Itching Pathways Related to the TRPV1 Channel, Histamine, PAR-2 and Serotonin (Cited in "Fundamental and Clinical Pharmacology, 02/2015"), The Malaysian Journal of Medical Sciences : MJMS, Vol.20, No.4, 5-12, Aug. 2013. (要約) The following four possible pathways for itching sensation have been suggested by recent reports. 1) Histaminergic TRPV1-positive pathway: Although histamine-positive nerve fibers cannot strictly be classified as "itch specific" due to their excitation also by pure algogens (making them itch-selective), the existence of a subpopulation of nociceptors responsible for itching is strongly suggested. Moreover, the TRPV1-expressing neurons have been suggested to be the main sensors and mediators of itching. 2) Histaminergic TRPV1-negative pathway: The scratching behavior caused by itching was not different between capsaicin-pre-treated and vehicle-treated (control) mast cell-rich NC mice. This result suggests the existence of a capsaicin-insensitive (TRPV1-negative) histaminergic pathway. 3) Non-histaminergic PAR-2 pathway: Protease-activated receptor 2 (PAR-2) has been shown to play a role in the itching of atopic dermatitis (AD). The itch evoked by cowhage (a non-histaminergic pruritogen that activates PAR-2) is very similar in characteristics to the itch evoked by conditions such as AD. 4) Non-histaminergic serotonin (5-HT) pathway: 5-HT alone applied to the human skin evokes an itching sensation and has been suggested to be involved in the itching associated with pruritic diseases, such as polycythemia vera and cholestasis. (キーワード) itch / TRPV1 / ヒスタミン (histamine) / PAR-2 / serotonin (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24043991 ● Summary page in Scopus @ Elsevier: 2-s2.0-84879558444 (PubMed: 24043991, Elsevier: Scopus)
5.	Akio Hiura and Hiroshi Nakagawa : Participation of Glias and Immune Cells in Neuropathic Pain, WebmedCentral, Vol.3, No.9: WMC003728, 1-8, Sep. 2012. (キーワード) Glias / TRP channels / Abnormal pain / Inflammatory soup / Acute noxious heat pain (徳島大学機関リポジトリ) ● Metadata: 103613 (出版サイトへのリンク) ● Publication site (DOI): 10.9754/journal.wmc.2012.003728 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.9754/journal.wmc.2012.003728 (徳島大学機関リポジトリ: 103613, DOI: 10.9754/journal.wmc.2012.003728)
6.	中川弘, 樋浦明夫 : カプサイシンの作用とそのレセプター，TRPV1, 及びそれらと関連する小型一次知覚ニューロンの概観, 四国歯学会雑誌, Vol.25, No.1, 1-10, 2012年7月. (要約) The specific actions of capsaicin on the small primary afferent neurons with regard to neuogenic inflammation and plasma extravasation are examined in this review. First, a short history of the study of capsaicin is introduced from the viewpoint of the efferent function of capsaicin-sensitive nerve fibers. Agonist (resiniferatoxin) and antagonists (capsazepine and ruthenium red) of capsaicin are refened, to better understand the action of the drug. The significance of the discovery of capsaicin receptor, TRPV1, and its characteristic features (polymodal receptor) are discussed based on recent reports, although the sensitization or desensitization mechanisms are not yet resolved. This review also briefly deals with the therapeutic use of capsaicin and its agonist and antagonist for relief pain. Whether or not capsaicin-sensitive nerve fibers are involved in itching is examined by recent literature survey. TRPV1-expressing nerve fibers were recently reported to be responsible for the itching sensation. Then, we proposed putative three possible itching pathways. The participation of pure sensoty nervefibers which exclusively transmit itchiness has not been found, as yet. (キーワード) カプサイシン / ヒスタミン / 痒み / TRPV1 (徳島大学機関リポジトリ) ● Metadata: 104975 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1050564287688027520 (徳島大学機関リポジトリ: 104975, CiNii: 1050564287688027520)
7.	Akio Hiura and Hiroshi Nakagawa : Roles of Glia, Immune Cells and the Thermo-TRP Channels, TRPV1, TRPA1 and TRPM8, in Pathological Pain, The Open Neuroscience Journal, Vol.6, 10-26, Mar. 2012. (要約) Studies into the interactions between glia/immune cells and neurons have focused on the induction of patho-logical (neuropathic or inflammatory) pain. Growing evidence of close relationships between peripheral and central glia and pathological pain has emerged during the last 2 decades. Numerous experimental studies have showed the release of cytokines and inflammatory neuropeptides from peripheral and central terminals of primary sensory neurons and from ac-tivated peripheral and central glia after nerve injury (crush, ligation or transection), which in turn act in a paracrine or autocrine manner. Cytokines induce the synthesis of algogens (pain-inducing substances such as prostaglandin) which leads to the primary (peripheral) or secondary (central) sensitization responsible for hyperalgesia or allodynia under in-flammatory conditions. The review has also highlighted the role of thermo transient receptor potential (TRP) channels TRPV1, TRPA1 and TRPM8 in the induction of pathological pain. The noxious heat sensor TRPV1 has an overt role in noxious heat hyperalgesia or allodynia, whereas TRPA1 and TRPM8 seem to have roles in noxious cold or mechanical al-lodynia, although results are inconsistent. Close mutual interrelationships between immune and glial cells and thermoTRP channels via cytokines or pro-inflammatory neuropeptides cannot be ignored when attempting to explain the induction and continuation of pathological pain. Investigations on the initial signals sent to the central area (superficial dorsal horn) remote from injured (or infectious) sites are a key point to clarify the mechanisms of pathological pain. (キーワード) chronic pain / glias / immune cells / thermo-TRP channels / TRPV1, TRPA1, TRPM8 (徳島大学機関リポジトリ) ● Metadata: 85867 (出版サイトへのリンク) ● Publication site (DOI): 10.2174/1874082001206010010 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1050564287688021504 ● Summary page in Scopus @ Elsevier: 2-s2.0-84859509685 (徳島大学機関リポジトリ: 85867, DOI: 10.2174/1874082001206010010, CiNii: 1050564287688021504, Elsevier: Scopus)
8.	Akio Hiura and Hiroshi Nakagawa : An overview of the action of capsaicin and its receptor , TRPV1, and their relations to small primary sensory neurons (In the Hot-Topic of "Anti-inflammatory and anti-allergy functions of capsaicin in association with its actions on primary sensory neurons" eds. by A. Hiura & G. Jancso), Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry, Vol.10, No.1, 2-9, Feb. 2011. (出版サイトへのリンク) ● Publication site (DOI): 10.2174/187152311795325505 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-79952753337 (DOI: 10.2174/187152311795325505, Elsevier: Scopus)
9.	中川弘, 樋浦明夫 : カプサイシンとTRP チャネル・サブファミリーおよびカプサイシン受容体 (TRPV1) と小型一次知覚ニューロンの特性とその相関関係, 四国歯学会雑誌, Vol.19, No.2, 197-218, 2007年1月. (徳島大学機関リポジトリ) ● Metadata: 289 (徳島大学機関リポジトリ: 289)
10.	Hiroshi Nakagawa and Akio Hiura : Capsaicin, transient receptor potential (TRP) protein subfamilies and the particular relationship between capsaicin receptors and small primary sensory neurons (Cited in " Physiology & Behavior, 90(5), 2007; American Journal of Physiology, 295(5), 2008; Journal of Neurochemistry, 112(2), 2009; Pharmacology & Therapeutics, 112(2), 2010; Acta Physiologica (Oxford), 2011; Human Physiology, 2012; FEBS Letter, 586(8), 2012; PLoS ONE, 8 (3), 2013; Frontiers in Neuroendocrinology, 04/2014; Molecular Pain, 10(1), 2014"), Anatomical Science International, Vol.81, No.3, 135-155, Sep. 2006. (要約) A number of subfamilies of the capsaicin receptor, collectively called TRP, have been reported since the discovery of vanilloid receptor 1 (VR1). The term 'TRP' is derived from 'transient receptor potential', which means the transient and rapid defect of reaction following long stimulation with light in the photoreceptor cells of mutant Drosophila. The common features of TRP family members are the centrally situated six transmembrane domain, in which an ion channel is located, three to four ankirin repeats at the N-terminus and a TRP domain comprising 25 amino acids at the C-terminus. The TRP family members are present in animals, including invertebrates and vertebrates, and in the cells in various tissues in individual animals. During evolution, the original TRP seems to have acquired a wide variety of functions related to sensing the inner or outer environment (e.g. to sensing light (Drosophila), osmolarity, protons, temperature, ligands and mechanical force). In mammals, the TRPV subfamily is exclusively expressed in small- to medium-sized primary sensory neurons that also co-express some chemical markers (i.e. isolectin B4 (IB4), fluoride-resistant acid phosphatase (FRAP), the P2X3 purinoceptor (a receptor provoked by ATP-induced nociception) and Ret, a glial cell line-derived neurotrophic factor receptor). There is a paradox in that regardless of the marked or complete loss of noxious, small sensory neurons (polymodal nociceptors) in mice treated with capsaicin during the neonatal period, as well as in VR1 (TRPV1)-deficient knock-out mice, the responses to noxious heat are normal. Regarding the paradox in mice treated with capsaicin as neonates, our explanation is that although capsaicin probably reduces the number of a subgroup of small neurons (IB4-, VR1+), the remaining IB4+ (VR1-) neurons can sense noxious heat normally. One working hypothesis is that mice lacking TRPV1/2 can sense noxious heat under normal conditions, presumably via another still unknown pathway, and TRPV1 has been suggested to be involved in noxious heat transduction under pathological conditions, such as inflammation and tissue injury. Further studies will be required to clarify these complexities. Mice treated with capsaicin as neonates would provide a model to investigate the above paradoxes, as would TRPV1-knock-out mice, although different mechanisms may be operating in the two models. (キーワード) Animals / Animals, Newborn / Capsaicin / Hot Temperature / Molecular Structure / Nerve Fibers, Unmyelinated / Neurons, Afferent / Reaction Time / TRPV Cation Channels / Transient Receptor Potential Channels (出版サイトへのリンク) ● Publication site (DOI): 10.1111/j.1447-073X.2006.00141.x (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16955665 ● Search Scopus @ Elsevier (PMID): 16955665 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1447-073X.2006.00141.x (DOI: 10.1111/j.1447-073X.2006.00141.x, PubMed: 16955665)

国際会議:

1.	Yuki Akazawa, Kimiko Ueda Yamaguchi, Hiroshi Nakagawa and Tomonori Iwasaki : A case of using a mouthpiece for bites in perioperative, Oral Care, Apr. 2022.
2.	Yukiko Kori, Kimiko Ueda Yamaguchi, Aimi Yamamoto, Hiroshi Nakagawa, Masami Yoshioka and Tsutomu Iwamoto : Evaluation of dental treatment adaptability for patient with pervasive developmental disorder(PDD), 22nd Congress of the International Association of Disability and Oral Health, Vol.15, No.3, 73, Berlin, Oct. 2014.
3.	Akio Hiura and Hiroshi Nakagawa : Paradoxical behaviors in response to nociceptive stimuli after inflammation in mice pretreated with capsaicin at neonate. 8th FENS FOrum of Neuroscience, Barcelona, July, 2012, 8th FENS Forum of Neuroscience, Barcelona, Jul. 2012.
4.	Akio Hiura and Hiroshi Nakagawa : Innervation of VR1-IR nerve fibers in the rat cornea, 16th International Congress of the IFAA, 334, Kyoto, Aug. 2004.
5.	Akio Hiura, Yoshiko Nakae and Hiroshi Nakagawa : Mouse DRG cell death by neonatal capsaicin treatment is mainly necrosis rather than apoptosis., Abstract Bookelt of the 15th International Symposium on Morphological Sciences, 227, Kyoto, Sep. 2000.
6.	Akio Hiura, Hiroshi Nakagawa and YOSHIHIRO KUBO : Sprouting from remained primary afferent neurons in the mouse superficial dorsal horn after neonatal capsaicin treatment, 29th Annual Meeting of the Society for Neuroscience, Miami Beach (USA), Oct. 1999.
7.	Akio Hiura, Hiroshi Nakagawa, Hiroshi Ishizuka and YOSHIHIRO KUBO : Effect of anti-NFG on the responses to noxious heat in mice treated with capsaicin at neonate., 28th Annuai Meeting of the Society for Neuroscience, 381, Los Angeles (USA), Nov. 1998.

国内講演発表:

1.	上田(山口) 公子, 赤澤友基, 河原林啓太, 宮嵜彩, 北村尚正, 長谷川智一, 中川弘, 岩﨑智憲 : 当院小児摂食嚥下外来における初診時調査, 小児歯科学雑誌, Vol.61, 53-54, 2023年2月.
2.	赤澤友基, 上田(山口) 公子, 中川弘, 岩﨑智憲 : 習癖による咬傷に対してマウスピースを使用した1例, 小児歯科学雑誌, Vol.60, 63-64, 2022年2月.
3.	岩﨑智憲, 前尾慶, 幸平若菜, 北村尚正, 河原林啓太, 宮嵜彩, 赤澤友基, 上田(山口) 公子, 中川弘, 長谷川智一 : 小児閉塞性睡眠時無呼吸への対応と展望, 徳島県小児保健協会総会並びに第62回講演会, 3, 2021年9月.
4.	赤澤友基, 岩田こころ, 黒厚子璃佳, 河原林啓太, 宮嵜彩, 藤島史帆, 森浩喜, 杉本明日菜, 北村尚正, 上田(山口) 公子, 中川弘, 長谷川智一, 岩本勉 : 本院小児歯科の初診患者の動向と主訴の変遷について, 徳島県歯科医学大会, 29, 2020年2月.
5.	山村佳子, 高石和美, 中川弘, 尼寺理恵, 岩本勉 : 当科におけるスペシャルニーズ患者の全身麻酔下歯科治療の臨床的検討, 第36回日本障害者歯科学会総会・学術大会, 2019年11月.
6.	杉本明日菜, 宮嵜彩, 河原林啓太, 岩田こころ, 黒厚子璃佳, 赤澤友基, 長谷川智一, 上田(山口) 公子, 北村尚正, 中川弘, 岩本勉 : 象牙芽細胞におけるピエゾ型イオンチャネル1(PIEZO1)の発現とその役割, 第37回日本小児歯科学会中四国地方会大会, 2018年11月.
7.	黒厚子璃佳, 杉本明日菜, 岩田こころ, 宮嵜彩, 河原林啓太, 藤島史帆, 赤澤友基, 北村尚正, 上田(山口) 公子, 尼寺理恵, 長谷川智一, 中川弘, 岩本勉 : 当科における5年間の障害児の初診時実態調査, 第37回日本小児歯科学会中四国地方会大会, 2018年11月.
8.	岩田こころ, 杉本明日菜, 黒厚子璃佳, 河原林啓太, 宮嵜彩, 藤島史帆, 北村尚正, 赤澤友基, 尼寺理恵, 上田(山口) 公子, 中川弘, 長谷川智一, 岩本勉 : 当科における初診患者の実態調査~平成における30年間の変遷~, 第37回日本小児歯科学会中四国地方会大会, 2018年11月.
9.	中川弘, 上田(山口) 公子, 北村尚正, 宮嵜彩, 岩本勉 : p-HPPHを分子標的とした新規口腔粘膜創傷治癒促進薬の開発ー歯肉線維芽細胞におけるp-HPPHによる細胞遊走の促進作用についてー, 障害者歯科, Vol.38, No.3, 288, 2017年10月.
10.	上田(山口) 公子, 赤澤友基, 北村尚正, 杉本明日菜, 長谷川智一, 中川弘, 岩本勉 : ヒト歯肉線維芽細胞におけるS-PRGフィラー抽出液による細胞遊走の促進, 小児歯科学雑誌, Vol.54, No.1, 145, 2015年10月.
11.	上田(山口) 公子, 益富由佳子, 尼寺理恵, 北村尚正, 山本愛美, 赤澤友基, 杉本明日菜, 白石真紀, 中川弘, 長谷川智一, 郡由紀子, 岩本勉 : 当科における小児の乳歯と永久歯の外傷の実態調査, 小児歯科学雑誌, Vol.53, No.2, 272, 2015年5月.
12.	山本愛美, 中川弘, 郡由紀子, 北村尚正, 杉本明日菜, 岩本勉 : 小児および障碍児・者への防災意識に関する研究, 小児歯科学雑誌, Vol.53, No.2, 330, 2015年5月.
13.	長谷川智一, 赤澤友基, 吉村善隆, 帖佐直幸, 浅川剛吉, 杉本明日菜, 北村尚正, 上田(山口) 公子, 中川弘, 白石真紀, 石崎明, 岩本勉 : 乳歯歯根膜由来細胞のSDF-1αを介した歯周組織恒常性に関わる細胞間相互作用, 小児歯科学雑誌, Vol.53, No.2, 222, 2015年.
14.	上田(山口) 公子, 郡由紀子, 中川弘, 山本愛美, 尼寺理恵, 真杉幸江, 岩本勉 : 広汎性発達障害児・者の歯科受診適応性と唾液アミラーゼ活性値を指標としたストレス評価, 障害者歯科, Vol.35, No.3, 504, 2014年10月.
15.	北村尚正, 中川弘, 杉本明日菜, 赤澤友基, 長谷川智一, 岩本勉 : 含歯性嚢胞の間葉系結合組織層が骨吸収に与える影響, 歯基礎誌抄録, 141, 2014年.
16.	中川弘, 山本愛美, 赤澤友基, 北村尚正, 長谷川智一, 岩本勉 : p-HPPHを分子標的としたフェニトインによる歯肉増殖症に対する新規治療薬の開発, 障害者歯科, Vol.35, No.3, 478, 2014年.
17.	赤澤友基, 長谷川智一, 久保-藤原百合, 北村尚正, 上田(山口) 公子, 中川弘, 岩本勉 : 当科にて長期的口腔内管理を行った伊藤白斑の1例, 小児歯科学雑誌, Vol.52, No.2, 354, 2014年.
18.	長谷川智一, 赤澤友基, 北村尚正, 上田(山口) 公子, 中川弘, 岩本勉 : 幼児期の小児の口蓋に発生した血管腫の1例, 小児歯科学雑誌, Vol.52, No.2, 355, 2014年.
19.	長谷川智一, 赤澤友基, 中川弘, 上田公子, 岩本勉 : 乳歯歯髄細胞による細胞走化性因子の調節機構, 小児歯科学雑誌, Vol.52, No.1, 125-126, 2014年.
20.	上田(山口) 公子, 山本愛美, 郡由紀子, 中川弘, 長谷川智一, 岩本勉 : 上顎中切歯の萌出遅延を起こした集合性歯牙腫の1症例, 第32回日本小児歯科学会中四国地方会大会, Vol.52, No.1, 180-181, 2013年11月.
21.	上田(山口) 公子, 郡由紀子, 山本愛美, 赤澤友基, 北村尚正, 中川弘, 長谷川智一, 岩本勉 : 過剰歯摘出によって歯の位置異常が改善した症例についての検討, 第31回日本小児歯科学会北日本地方会大会, Vol.52, No.1, 122-123, 2013年10月.
22.	中川弘, 山本愛美, 郡由紀子, 上田(山口) 公子, 赤澤友基, 北村尚正, 長谷川智一, 岩本勉 : 歯科治療恐怖症患者に応用した心理検査結果の経時的変化について, 障害者歯科, Vol.34, No.3, 375, 2013年10月.
23.	山本愛美, 有田憲司, 郡由紀子, 上田(山口) 公子, 中川弘, 岩本勉 : 当院小児摂食外来を受診した初診患児の実態調査, 第30回日本障害者歯科学会学術大会, 2013年10月.
24.	山本愛美, 有田憲司, 郡由紀子, 上田(山口) 公子, 中川弘, 岩本勉 : 当院小児摂食外来を受診した初診患児の実態調査, 障害者歯科, Vol.34, No.3, 289, 2013年9月.
25.	中川弘, 山本愛美, 郡由紀子, 上田(山口) 公子, 赤澤友基, 北村尚正, 長谷川智一, 岩本勉 : 歯科治療恐怖症患者に応用した心理検査結果の経時的変化について, 障害者歯科, Vol.34, No.3, 375, 2013年9月.
26.	山本愛美, 有田憲司, 郡由紀子, 上田(山口) 公子, 中川弘, 岩本勉 : 当院小児摂食外来を受診した初診患児の実態調査, 障害者歯科, Vol.34, No.3, 289, 2013年9月.
27.	上田公子, 山本愛美, 郡由紀子, 中川弘, 長谷川智一, 岩本勉 : 上顎中切歯の萌出遅延を起こした集合性歯牙腫の1症例, 小児歯科学雑誌, Vol.52, No.1, 180-181, 2013年.
28.	中川弘, 山本愛美, 郡由紀子, 赤澤友基, 北村尚正, 長谷川智一, 岩本勉 : 歯科治療恐怖症患者に応用した心理検査結果の経時的変化について, 障害者歯科, Vol.34, No.3, 375, 2013年.
29.	尼寺理恵, 郡由紀子, 中川弘, 山本愛美 : 障害者施設での実習前後における学生の意識変化, 障害者歯科, Vol.33, No.3, 412, 2012年9月.
30.	中川弘, 山本愛美, 尼寺理恵, 郡由紀子 : TRPチャネルを用いた新しい局所麻酔薬の開発 -TRPV1チャネルとTRPA1チャネルの比較ー, 障害者歯科, Vol.32, No.3, 230, 2011年11月. (キーワード) TRPV1チャネル / TRPA1チャネル / QX-314 / カプサイシン (capsaicin) / アリルイソチオシアネート
31.	山本愛美, 中川弘, 三留雅人 : 自閉症患者へのブラッシング指導における継続的な視覚支援による学習効果, 小児歯科学雑誌, Vol.48, No.5, 634, 2010年11月.
32.	西野瑞穗, 粟飯原靖司, 有田憲司, 原田桂子, 加藤邦子, 岡本多恵, 西田文彦, 中川弘, 金城聡子, 田村由紀, 菊地賢司, グァダルーペアルバラードラリナガ : 貼付型表面麻酔剤の考案とその使用成績, 歯界展望(臨時増刊), Vol.71, No.3, 818, 1988年2月.

その他・研究会:

1.	樋浦明夫, 中川弘 : QX-314 はカプシエイトまたはアナンドアミドとの併用投与で侵害熱刺激を抑制する, 平成24年度生理学研究所研究会「痛み研究の新たな展開」(岡崎), 2012年12月.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

ジンジパインを分子標的としたダウン症候群の退行現象に対する新規治療法の開発 (研究課題/領域番号: 20K10204 )
p-HPPHを分子標的とした新規創傷治癒促進薬の開発 (研究課題/領域番号: 16K11805 )
TRPA1チャネル阻害剤を応用した薬物性歯肉増殖症の治療薬の開発 (研究課題/領域番号: 25463180 )
広汎性発達障害児者の歯科受診における効果的な行動調整支援プログラムの開発 (研究課題/領域番号: 24653296 )
新規歯科用局所麻酔薬の開発と無針注射器(シリジェット)への応用について (研究課題/領域番号: 22592289 )
顎補綴患者の発音・嚥下機能回復に対する評価法の確立 (研究課題/領域番号: 15592054 )
発音・嚥下機能回復が確実にできる補綴装置製作法 (研究課題/領域番号: 12671890 )
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講師 : 中川弘