徳島大学 / 教育研究者総覧 --- 田端厚之

個人のホームページ

https://researchmap.jp/tabataatsushi (researchmap)

https://orcid.org/0000-0002-4896-1738 (ORCID)

専門分野

○	微生物学 (Microbiology)

研究テーマ

○	微生物感染と宿主応答メカニズムに関する研究 (細菌毒素, 感染機構, 宿主応答)

著書・論文

著書:

1.	橋爪正樹, 右手浩一, 井﨑ゆみ子, 大野将樹, 中野晋, 溝渕啓, 玉谷純二, 下村直行, 上田隆雄, 上野勝利, 長尾文明, 橋本親典, 武藤裕則, 渡邉健, 木戸崇博, 片山哲郎, 山下陽子, 田端厚之, 佐々木由香, 島木美香, 音井威重, 金丸芳, 服部武文, 濵野龍夫, 宮脇克行, 安間了, 西山賢一, 青矢睦月 : 安全マニュアル, 2021年4月.
2.	大西徳生, 魚崎泰弘, 前田健一, 獅々堀正幹, 中野晋, 多田吉宏, 玉谷純二, 下村直行, 三神厚, 倉科昌, 中村真紀, 杉山茂, 小澤将人, 山本裕紹, 黒田トクエ, 田端厚之, 佐々木由香, 島木美香 : 安全マニュアル, 工学部, 徳島, 2010年2月.

学術論文(審査論文):

1.	Kazuto Ohkura, Atsushi Tabata and Yoshihiro Uto : Interactive Analysis of UTX-114 Family With EGFR-tyk: Molecular Features of Acetyl Glycosylated Gefitinib., Anticancer Research, Vol.44, No.8, 3587-3591, 2024. (要約) Acetyl glucose adducts (UTX-114, -115, and -116) were prepared from gefitinib, and their characteristics (e.g., anticancer activity, structural property) were analyzed. Cytotoxicity and radiosensitizing properties of the UTX-114 family were examined using A431 cells. Supramolecular associations between the UTX-114 family compounds and the tyrosine kinase domain of epidermal growth factor receptor (EGFR-tyk) were also examined. The interactive analyses of the UTX-114 family compounds with EGFR-tyk were performed using docking simulation technique. The UTX-114 family showed a similar cytotoxicity as gefitinib, yielding IC values of 31.2 μM (gefitinib), 34.3 μM (UTX-114), 36.8 μM (UTX-115), and 39.4 μM (UTX-116). The EGFR-tyk inhibition ratios (IR) of UTX-114, -115, and -116 to gefitinib were 1.515, 0.983, and 0.551, respectively. The EGFR-tyk inhibitory activity of UTX-114 was higher than that of gefitinib. UTX-114 also showed the highest radiosensitizing activity among the tested compounds. UTX-114 expressed 1841 conformers (-8.989~15.718 kcal/mol) with the solvation free energy (dGW) of UTX-114 decreasing with increasing conformational energy, ranging between -354.955~ -260.815 kJ/mol. Interactive energies of gefitinib, UTX-114, -115, and -116 with EGFR-tyk were -123.640, -144.053, -120.830, and -124.658 kcal/mol, respectively. UTX-114 yielded the lowest interaction energy with EGFR-tyk among tested compounds. Given the association behavior between UTX-114 and EGFR-tyk, along with its other observed properties, UTX-114 appears to be a viable therapeutic possibility. (キーワード) EGFR-tyk inhibition / 分子設計 (molecular design) / UTX-114 / docking simulation / gefitinib / radiosensitizing activity (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.17181 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39060048 ● Search Scopus @ Elsevier (PMID): 39060048 ● Search Scopus @ Elsevier (DOI): 10.21873/anticanres.17181 (DOI: 10.21873/anticanres.17181, PubMed: 39060048)
2.	Fumitoshi Yagishita, Tetsuro Katayama, Yuta Kawamura, WATANABE Guran, ABE Sota, OGAWA Itsuki, Atsushi Tabata, Yasushi Yoshida, Hyuma Masu, Shoko Ueta, Yukihiro Arakawa, Keiji Minagawa, Akihiro Furube and Yasushi Imada : Blue Luminescent Boron Complexes Based on N,N-Type Imidazo[1,5-a]pyridine Ligand for Mitochondrial Imaging, Asian Journal of Organic Chemistry, 2024. (出版サイトへのリンク) ● Publication site (DOI): 10.1002/ajoc.202400189 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1002/ajoc.202400189 (DOI: 10.1002/ajoc.202400189)
3.	YAMADA Takuya, YAMAMORI Yugo, Matsuda Nanami, Hideaki Nagamune, Kazuto Ohkura, Toshifumi Tomoyasu and Atsushi Tabata : Streptolysin S induces pronounced calcium-ion influx-dependent expression of immediate early genes encoding transcription factors, Scientific Reports, Vol.13, No.13720, 2023. (キーワード) 細胞溶解毒素 (cytolysin) / 連鎖球菌 (Streptococcus)
4.	Toshifumi Tomoyasu, Airi Matsumoto, Ayuko Takao, Atsushi Tabata and Hideaki Nagamune : A simple method to differentiate three classes of cholesterol-dependent cytolysins., Journal of Microbiological Methods, Vol.207, 2023. (要約) Cholesterol-dependent cytolysins (CDCs) are proteinaceous toxins widely distributed in gram-positive pathogenic bacteria. CDCs can be classified into three groups (I-III) based on the mode of receptor recognition. Group I CDCs recognize cholesterol as their receptor. Group II CDC specifically recognizes human CD59 as the primary receptor on the cell membrane. Only intermedilysin from Streptococcus intermedius has been reported as a group II CDC. Group III CDCs recognize both human CD59 and cholesterol as receptors. CD59 contains five disulfide bridges in its tertiary structure. Therefore, we treated human erythrocytes with dithiothreitol (DTT) to inactivate CD59 on membranes. Our data showed that DTT treatment caused a complete loss of recognition of intermedilysin and an anti-human CD59 monoclonal antibody. In contrast, this treatment did not affect the recognition of group I CDCs, judging from the fact that DTT-treated erythrocytes were lysed with the same efficiency as mock-treated human erythrocytes. The recognition of group III CDCs toward DTT-treated erythrocytes was partially reduced, and these results are likely due to the loss of human CD59 recognition. Therefore, the degree of human CD59 and cholesterol requirements of uncharacterized group III CDCs frequently found in Mitis group streptococci can be easily estimated by comparing the amounts of hemolysis between DTT-treated and mock-treated erythrocytes. (キーワード) 細菌毒素 (bacterial toxins) / Cytotoxins / Cell Membrane / Erythrocytes / コレステロール (cholesterol) (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.mimet.2023.106696 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36898586 ● Search Scopus @ Elsevier (PMID): 36898586 ● Search Scopus @ Elsevier (DOI): 10.1016/j.mimet.2023.106696 (DOI: 10.1016/j.mimet.2023.106696, PubMed: 36898586)
5.	Shuto Yokohata, Kazuto Ohkura, Hideaki Nagamune, Toshifumi Tomoyasu and Atsushi Tabata : Human serum albumin stabilizes streptolysin S activity secreted in the extracellular milieu by streptolysin S-producing streptococci., Microbiology and Immunology, Vol.67, No.2, 58-68, 2022. (要約) Anginosus group streptococci (AGS) are opportunistic pathogens of the human oral cavity; however, their pathogenicity has not been discussed in detail. Oral streptococci live in the gingival sulcus, from where they can easily translocate into the bloodstream due to periodontal diseases and dental treatment and cause hazardous effects on the host through their virulence factors. Streptolysin S (SLS), a pathogenic factor produced by β-hemolytic species/strains belonging to AGS, plays an important role in damaging host cells. Therefore, we investigated the SLS-dependent cytotoxicity of β-hemolytic Streptococcus anginosus subsp. anginosus (SAA), focusing on different growth conditions such as in the bloodstream. Consequently, SLS-dependent hemolytic activity/cytotoxicity in the culture supernatant of β-hemolytic SAA was stabilized by blood components, particularly human serum albumin (HSA). The present study suggests that the secreted SLS, not only from β-hemolytic SAA, but also from other SLS-producing streptococci, is stabilized by HSA. As HSA is the most abundant protein in human plasma, the results of this study provide new insights into the risk of SLS-producing streptococci which can translocate into the bloodstream. (キーワード) Humans / Streptolysins / Serum Albumin, Human / Streptococcus pyogenes / Virulence / Virulence Factors / Bacterial Proteins (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1348-0421.13042 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36478453 ● Search Scopus @ Elsevier (PMID): 36478453 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.13042 (DOI: 10.1111/1348-0421.13042, PubMed: 36478453)
6.	Atsushi Tabata, Airi Matsumoto, Ai Fujimoto, Kazuto Ohkura, Takuya Ikeda, Hiroki Oda, Shuto Yokohata, Miho Kobayashi, Toshifumi Tomoyasu, Ayuko Takao, Hisashi Ohkuni and Hideaki Nagamune : Dual functions of discoidinolysin, a cholesterol-dependent cytolysin with N-terminal discoidin domain produced from strain Nm-76., Journal of Oral Microbiology, Vol.14, No.1, 2105013, 2022. (要約) Some strains of exhibit β-hemolysis due to the β-hemolytic activity of cholesterol-dependent cytolysin (CDC). Recently, a gene encoding an atypical lectinolysin-related CDC was found in strain Nm-76. However, the product of this gene remains uncharacterized. We aimed to characterize this atypical CDC and its molecular functions and contribution to the pathogenicity of strain Nm-76. Phylogenetic analysis of the CDC gene was conducted based on the web-deposited information. The molecular characteristics of CDC were investigated using a gene-deletion mutant strain and recombinant proteins expressed in . The gene encoding CDC found in Nm-76 and its homolog are distributed among many strains. This CDC is phylogenetically different from other previously characterized CDCs, such as -derived human platelet aggregation factor (Sm-hPAF)/lectinolysin and mitilysin. Because this CDC possesses an additional N-terminal domain, including a discoidin motif, it was termed discoidinolysin (DLY). In addition to the preferential lysis of human cells, DLY displayed N-terminal domain-dependent facilitation of human erythrocyte aggregation and intercellular associations between human cells. DLY functions as a hemolysin/cytolysin and erythrocyte aggregation/intercellular association molecule. This dual-function DLY could be an additional virulence factor in . (出版サイトへのリンク) ● Publication site (DOI): 10.1080/20002297.2022.2105013 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35937899 ● Search Scopus @ Elsevier (PMID): 35937899 ● Search Scopus @ Elsevier (DOI): 10.1080/20002297.2022.2105013 (DOI: 10.1080/20002297.2022.2105013, PubMed: 35937899)
7.	Kazuto Ohkura, Atsushi Tabata, Yoshihiro Uto and Hitoshi Hori : Molecular Interaction Between Boron Tracedrug UTX-51 Derivatives and Bovine Serum Albumin: Application to an Analytical Model of AGEs Destruction by Thermal Neutron Irradiation, Anticancer Research, Vol.42, No.8, 4017-4023, 2022. (要約) Boron tracedrugs possess global molecular tracking abilities and localized destructive power. We investigated the molecular properties of synthesized boron tracedrugs, including UTX-51, and their interactions with the advanced glycation end-product (AGE)-related protein bovine serum albumin (BSA). A conformational analysis of the compounds used in the present study was performed using CAChe (Fujitsu Inc., Tokyo, Japan) and the degree of stereo-hydrophobicity of the conformers obtained was verified using Mopac (Fujitsu Inc.). The interactive properties of global minimum conformers of the derivatives tested with BSA were assessed using Molegro Virtual Docker (CLC bio., Aarhus, Denmark). Among the compounds investigated, UTX-51 was confirmed to interact with BSA based on the formation of hydrogen bonds between BSA and UTX-51. UTX-51 is a promising boron tracedrug and can be used as the lead structure for developing a therapeutic agent for AGE-related diseases, including cancer. (キーワード) BSA / Boron tracedrugs / 分子設計 (molecular design) / UTX-51 (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.15898 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35896254 ● Search Scopus @ Elsevier (PMID): 35896254 ● Search Scopus @ Elsevier (DOI): 10.21873/anticanres.15898 (DOI: 10.21873/anticanres.15898, PubMed: 35896254)
8.	Atsushi Tabata, Rina Shirai, Haruka Miki, Yukihiro Nishikawa, Tatsuya Kashima, Tomomi Aoyama, Shu Murakami, Momoyo Azuma, Toshifumi Tomoyasu and Hideaki Nagamune : Hapten-labeled fusion-polymerase chain reaction of multiple marker genes for the application of immunochromatographic test., Journal of Bioscience and Bioengineering, Vol.134, No.1, 70-76, 2022. (要約) A variety of methods have been reported using polymerase chain reaction (PCR)-based nucleic acid testing (NAT) because of its potential to be used in highly sensitive inspection systems. Among these NATs, fusion-PCR (also called as overlap-extension-PCR) has been focused on this study and adopted to generate the fused amplicon composed of plural marker gene fragments for detection. Generally, conventional agarose gel electrophoresis followed by gel staining is employed to check the PCR results. However, these are time-consuming processes that use specific equipment. To overcome these disadvantages, the immunochromatographic test (ICT) for the detection of PCR amplicons with hapten-labels that were generated by PCR using hapten-labeled primers was also adopted in this study. Based on these concepts, we constructed the systems of hapten-labeled fusion-PCR (HL-FuPCR) followed by ICT (HL-FuPCR-ICT) for the two and three marker genes derived from pathogenic microbe. As a result, we successfully developed a two marker genes system for the pathogenic influenza A virus and a three marker genes system for the penicillin-resistant Streptococcus pneumoniae. These detection systems of HL-FuPCR-ICT are characterized by simple handling and rapid detection within few minutes, and also showed the results as clear lines. Thus, the HL-FuPCR-ICT system introduced in this study has potential for use as a user-friendly inspection tool with the advantages especially in the detection of specific strains or groups expressing the characteristic phenotype(s) such as antibiotic resistance and/or high pathogenicity even in the same species. (キーワード) DNA Primers / Haptens / Immunologic Tests / Polymerase Chain Reaction / Sensitivity and Specificity (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.jbiosc.2022.03.006 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35450786 ● Search Scopus @ Elsevier (PMID): 35450786 ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbiosc.2022.03.006 (DOI: 10.1016/j.jbiosc.2022.03.006, PubMed: 35450786)
9.	Hiroki Nakai, Daiu Akiyama, Yoshiaki Taniguchi, Iori Kishinobu, Hiromichi Wariishi, Yasuhide Ohno, Masao Nagase, Takuya Ikeda, Atsushi Tabata and Hideaki Nagamune : Charge-independent protein adsorption characteristics of epitaxial graphene field-effect transistor on SiC substrate, Journal of Applied Physics, Vol.130, No.7, 074502-1-074502-8, 2021. (キーワード) グラフェン (graphene) (徳島大学機関リポジトリ) ● Metadata: 116163 (出版サイトへのリンク) ● Publication site (DOI): 10.1063/5.0054688 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1063/5.0054688 (徳島大学機関リポジトリ: 116163, DOI: 10.1063/5.0054688)
10.	Keita Hoshi, Kazuma Kusumoto, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Synthesis of D-π-A type benzothiazolepyridinium salt composite and its application as photo-degradation agent for amyloid fibrils, Bioorganic & Medicinal Chemistry Letters, Vol.50, 128324, 2021. (要約) We have synthesized a cyan fluorescent benzothiazole-pyridinium salt composite based on D-p-A architecture. This salt was found to work as not only a two- and three-photon excitable fluorophore but also a degradation agent against amyloid fibrils under LED irradiation conditions. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bmcl.2021.128324 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34403727 ● Search Scopus @ Elsevier (PMID): 34403727 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bmcl.2021.128324 (DOI: 10.1016/j.bmcl.2021.128324, PubMed: 34403727)
11.	Kazuto Ohkura, Yohei Tatematsu and Atsushi Tabata : Construction of a Drug Release Evaluation System: Application of Mitochondrial Respiration to Monitor Drug Release, Anticancer Research, Vol.41, No.8, 4083-4088, 2021. (要約) Efficient drug encapsulation and regulation of drug release are important factors for sustained drug release and application for release-controlled anti-cancer and anti-inflammatory drug delivery. In the present study, a direct evaluation system for drug-release from model carrier (e.g., alginate-gel beads) was examined using the mitochondrial oxygen consumption rate as an index. Alginate-gel beads were coated with the uncoupler SF6847 (SF beads) and used as a model microparticle-type drug. The real-time monitoring of SF6847 release from prepared alginate-gel beads was performed using the mitochondrial oxygen consumption rate. Release profiles of nonsteroidal anti-inflammatory drugs [NSAIDs, mefenamic acid (MEF) and diclofenac (DIC)] from alginate-gel beads as well as SF beads were investigated using the real time monitoring system. SF6847 release from alginate-gel beads was clearly detected using the rat liver mitochondrial oxygen consumption rate. The release features of MEF and DIC from alginate-gel beads were defined by the present trial monitoring system, and these NSAIDs exhibited different release profiles. The present drug monitoring system detected released drugs, and the release profile reflected the molecular properties of the test drugs. This system may be applied to the design and development of precise sustained drug release systems (e.g., anti-cancer and anti-inflammatory drugs). (キーワード) Alginates / Animals / Anti-Inflammatory Agents, Non-Steroidal / Cell Respiration / Diclofenac / Drug Carriers / Drug Liberation / Mefenamic Acid / Mitochondria, Liver / Nitriles / Oxygen / Rats / Uncoupling Agents (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.15210 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34281878 ● Search Scopus @ Elsevier (PMID): 34281878 ● Search Scopus @ Elsevier (DOI): 10.21873/anticanres.15210 (DOI: 10.21873/anticanres.15210, PubMed: 34281878)
12.	Keita Hoshi, Masami Itaya, Koki Tahara, Airi Matsumoto, Atsushi Tabata, Hideaki Nagamune, Yasushi Yoshida, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Tetsuro Katayama, Akihiro Furube, Keiji Minagawa, Yasushi Imada and Fumitoshi Yagishita : Two-photon excitable boron complex based on tridentate imidazo[1,5-a]pyridine ligand for heavy- atom-free mitochondria-targeted photodynamic therapy, RSC Advances, Vol.11, 26403-26407, 2021. (徳島大学機関リポジトリ) ● Metadata: 116625 (出版サイトへのリンク) ● Publication site (DOI): 10.1039/d1ra05059a (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1039/d1ra05059a (徳島大学機関リポジトリ: 116625, DOI: 10.1039/d1ra05059a)
13.	Airi Matsumoto, Atsushi Tabata, Kazuto Ohkura, Hiroki Oda, Chihiro Kodama, Hisashi Ohkuni, Ayuko Takao, Ken Kikuchi, Toshifumi Tomoyasu and Hideaki Nagamune : Molecular characteristics of an adhesion molecule containing cholesterol-dependent cytolysin-motif produced by mitis group streptococci, Microbiology and Immunology, Vol.65, No.2, 61-75, 2021. (要約) Streptococcus pseudopneumoniae (SPpn) is a relatively new species closely related to S. pneumoniae (SPn) and S. mitis (SM) belonging to the Mitis group of the genus Streptococcus (MGS). Although genes encoding various pneumococcal virulence factors have been observed in the SPpn genome, the pathogenicity of SPpn against human, including the roles of virulence factor candidates, is still unclear. The present study focused on and characterized a candidate virulence factor previously reported in SPpn with deduced multiple functional domains, such as lipase domain, two lectin domains, and cholesterol-dependent cytolysin-related domain using various recombinant proteins. The gene was found not only in SPpn but also in the strains of SM and SPn. Moreover, the gene product was expressed in the gene-positive strains as secreted and cell-bound forms. The recombinant of gene product showed lipase activity and human cell-binding activity depending on the function of lectin domain(s), but no hemolytic activity. Thus, based on the distribution of the gene within the MGS and its molecular function, the gene product was named mitilectin (MLC) and its contribution to the potential pathogenicity of the MLC-producing strains was investigated. Consequently, the treatment with anti-MLC antibody and the mlc gene-knockout significantly reduced the human cell-binding activity of MLC-producing strains. Therefore, the multifunctional MLC was suggested to be important as an adhesion molecule in considering the potential pathogenicity of the MLC-producing strains belonging to MGS, such as SPpn and SM. (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1348-0421.12868 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33331679 ● Search Scopus @ Elsevier (PMID): 33331679 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.12868 (DOI: 10.1111/1348-0421.12868, PubMed: 33331679)
14.	Atsushi Tabata, Hisashi Ohkuni, Yasuhiko Itoh, Yoshitaka Fukunaga, Toshifumi Tomoyasu and Hideaki Nagamune : Complete Genome Sequence of Streptococcus mitis Strain Nm-65, Isolated from a Patient with Kawasaki Disease, Microbiology Resource Announcements, Vol.10, No.1, e01239-20, 2021. (要約) Nm-65 is a human commensal streptococcal strain of the mitis group that was isolated from the tooth surface of a patient with Kawasaki disease. The complete genome sequence of Nm-65 was obtained by means of hybrid assembly, using two next-generation sequencing data sets. The final assembly size was 2,085,837 bp, with 2,039 coding sequences. (徳島大学機関リポジトリ) ● Metadata: 116462 (出版サイトへのリンク) ● Publication site (DOI): 10.1128/MRA.01239-20 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33414340 ● Search Scopus @ Elsevier (PMID): 33414340 ● Search Scopus @ Elsevier (DOI): 10.1128/MRA.01239-20 (徳島大学機関リポジトリ: 116462, DOI: 10.1128/MRA.01239-20, PubMed: 33414340)
15.	Keita Hoshi, Yohei Sanagawa, Ryuta Umebayashi, Atsushi Tabata, Hideaki Nagamune, Eiji Hase, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Synthesis and Optical Property of Quadrupolar Pyridinium Salt and Its Application as Bioimaging Agent, Chemistry Letters, Vol.49, No.12, 1487-1489, 2020. (出版サイトへのリンク) ● Publication site (DOI): 10.1246/cl.200604 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1246/cl.200604 (DOI: 10.1246/cl.200604)
16.	Eiji Hase, Hiroki Takanari, Keita Hoshi, Masaki Okamoto, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Yoshida, Keiji Minagawa, Yasuhiko Kawamura, Yasushi Imada and Fumitoshi Yagishita : Two- and three-photon excitable quaternized imidazo[1,2-a]pyridines for mitochondrial imaging and potent cancer therapy agent, Organic & Biomolecular Chemistry, Vol.18, No.38, 7571-7576, 2020. (出版サイトへのリンク) ● Publication site (DOI): 10.1039/D0OB01585G (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32940324 ● Search Scopus @ Elsevier (PMID): 32940324 ● Search Scopus @ Elsevier (DOI): 10.1039/D0OB01585G (DOI: 10.1039/D0OB01585G, PubMed: 32940324)
17.	Kazuto Ohkura, Atsushi Tabata, Yoshihiro Uto and Hitoshi Hori : Effect of Isomerization of TX-2036 Derivatives on the Interaction With Tyrosine Kinase Domain of EGF Receptor, Anticancer Research, Vol.40, No.8, 4675-4680, 2020. (要約) From the design and synthesis of enantiomers, we can expect to obtain two compounds with different pharmacokinetics and pharmacological activities at the same time, which is thought to lead to the development of efficient anticancer agents. Chiral-2-nitroimidazole TX-2036 derivatives exhibit stereo-configuration (R- and S-configuration)-dependent tyrosine kinase inhibitory activity, and the activity of the tyrosine kinase domain of EGF receptor (EGFR-tyk) is suppressed. In order to clarify the reason why the effects on EGFR-tyk activity differ depending on stereoisomers, we tried to analyze the interaction between each TX-2036 derivative and EGFR-tyk. The 2-nitroimidazole-based radiosensitizer TX-2036 series were synthesized and their molecular features were examined using protein kinase inhibition assay and molecular structural analysis. R-configured TXs (TX-2043, -2030, and -2036) exhibited more potent protein kinase inhibitory activity than S-configured TXs (TX-2044, - 2031, and -2037), and the IC value of TX-2036 was 1.8 μM. R-configured TXs interacted with Lys and Thr of EGFR-tyk. The combinations of amino acid residues targeted by the S-configured TXs were different from each other (Ile and Thr (TX-2044), Ser, Thr, and Thr (TX-2031), Gly, Cys, and Thr (TX-2037)). Preparing a series of isomers with different target sites was considered beneficial when the target was mutated. (キーワード) EGF receptor / 分子設計 (molecular design) / TX-2036 derivatives / 構造 (structure) (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.14466 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32727791 ● Search Scopus @ Elsevier (PMID): 32727791 ● Search Scopus @ Elsevier (DOI): 10.21873/anticanres.14466 (DOI: 10.21873/anticanres.14466, PubMed: 32727791)
18.	Daisuke Takamatsu, Kayo Okumura, Atsushi Tabata, Mariko Okamoto and Masatoshi Okura : Transcriptional regulator SpxA1a controls the resistance of the honey bee pathogen Melissococcus plutonius to the antimicrobial activity of royal jelly, Environmental Microbiology, Vol.22, No.7, 2736-2755, 2020. (要約) Royal jelly (RJ), a brood food of honey bees, has strong antimicrobial activity. Melissococcus plutonius, the causative agent of European foulbrood of honey bees, exhibits resistance to this antimicrobial activity and infects larvae orally. Among three genetically distinct groups (CC3, CC12 and CC13) of M. plutonius, CC3 strains exhibit the strongest RJ resistance. In this study, to identify genes involved in RJ resistance, we generated an RJ-susceptible derivative from a highly RJ-resistant CC3 strain by UV mutagenesis. Genome sequence analysis of the derivative revealed the presence of a frameshift mutation in the putative regulator gene spxA1a. The deletion of spxA1a from a CC3 strain resulted in increased susceptibility to RJ and its antimicrobial component 10-hydroxy-2-decenoic acid. Moreover, the mutant became susceptible to low-pH and oxidative stress, which may be encountered in brood foods. Differentially expressed gene analysis using wild-type and spxA1a mutants revealed that 45 protein-coding genes were commonly upregulated in spxA1a-positive strains. Many upregulated genes were located in a prophage region, and some highly upregulated genes were annotated as universal/general stress proteins, oxidoreductase/reductase, chaperons and superoxide dismutase. These results suggest that SpxA1a is a key regulator to control the tolerance status of M. plutonius against stress in honey bee colonies. (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1462-2920.15125 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32519428 ● Search Scopus @ Elsevier (PMID): 32519428 ● Search Scopus @ Elsevier (DOI): 10.1111/1462-2920.15125 (DOI: 10.1111/1462-2920.15125, PubMed: 32519428)
19.	Atsushi Tabata, Hisashi Ohkuni, Haruka Hino, Takuya Saigo, Chihiro Kodama, QING TANG, Toshifumi Tomoyasu, Yoshitaka Fukunaga, Yasuhiko Itoh and Hideaki Nagamune : Cytotoxic property of Streptococcus mitis strain producing two different types of cholesterol-dependent cytolysins, Infection, Genetics and Evolution, Vol.85, 104483, 2020. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.meegid.2020.104483 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32731044 ● Summary page in Scopus @ Elsevier: 2-s2.0-85088967403 (DOI: 10.1016/j.meegid.2020.104483, PubMed: 32731044, Elsevier: Scopus)
20.	高尾亞由子, 友安俊文, 田端厚之, 長宗秀明, 前田伸子 : A photometric pH assay for microplate bacterial cultures., Journal of Microbiological Methods, Vol.172, 1-3, 2020年. (要約) A photometric pH assay for sugar-fermenting bacterial culture on a 96-well plate was developed. This assay can save time and effort in repeat handlings. Its use could decrease the risk of bacterial contamination in measurement devices and leakage into the environment. The assay's pH estimation range was pH 4.2-7.6. (キーワード) chemical indicators / 発酵 (fermentation) / pH assay / photometric method / microbial culture (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.mimet.2020.105910 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32229266 ● Summary page in Scopus @ Elsevier: 2-s2.0-85082519032 (DOI: 10.1016/j.mimet.2020.105910, PubMed: 32229266, Elsevier: Scopus)
21.	Miho Kobayashi, Madoka Nishimura, Mina Kawamura, Norio Kamemura, Hideaki Nagamune and Atsushi Tabata : Change in membrane potential induced by streptolysin O, a pore-forming toxin: flow cytometric analysis using a voltage-sensitive fluorescent probe and rat thymic lymphocytes, Microbiology and Immunology, Vol.64, No.1, 10-22, 2020. (要約) Streptolysin O (SLO) is a bacterial pore-forming toxin that is employed to permeabilize cell membranes in some biological experiments. SLO forms various types of pores with different shapes, increasing membrane ion permeability and subsequently inducing changes in membrane potential. To characterize the pores formed by SLO, the changes in membrane potential induced by SLO in rat lymphocytes were considered using flow cytometry with a voltage-sensitive fluorescent probe, bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Oxonol). SLO caused three types of membrane potential responses accessed with Oxonol. One type induces a great decrease in Oxonol fluorescence (large hyperpolarization) that may be elicited via the increase of Ca -dependent K permeability by SLO-induced influx of external Ca . A second type is an increase in Oxonol fluorescence (depolarization) that may be caused by a nonspecific increase in membrane cation permeability. The third type is a small decrease in Oxonol fluorescence (small hyperpolarization), probably via an increase in Cl permeability. That SLO transitionally changes membrane ion permeability may have implications in the pathology of pyogenic group streptococci infections in which SLO is thought to be one of the key virulence factors. (キーワード) Animals / Bacterial Proteins / Bacterial Toxins / Barbiturates / Calcimycin / Calcium / Cell Membrane / Cell Membrane Permeability / Disease Models, Animal / Flow Cytometry / Fluorescent Dyes / Lymphocytes / Male / Membrane Potentials / Rats / Rats, Wistar / Streptolysins (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1348-0421.12748 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31584206 ● Search Scopus @ Elsevier (PMID): 31584206 ● Search Scopus @ Elsevier (DOI): 10.1111/1348-0421.12748 (DOI: 10.1111/1348-0421.12748, PubMed: 31584206)
22.	Fumitoshi Yagishita, Jun-ichi Tanigawa, Chiho Nii, Atsushi Tabata, Hideaki Nagamune, Hiroki Takanari, Yasushi Imada and Yasuhiko Kawamura : Fluorescent Imidazo[1,5-a]pyridinium Salt for a Potential Cancer Therapy Agent, ACS Medicinal Chemistry Letters, Vol.10, No.8, 1110-1114, 2019. (出版サイトへのリンク) ● Publication site (DOI): 10.1021/acsmedchemlett.9b00034 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1021/acsmedchemlett.9b00034 (DOI: 10.1021/acsmedchemlett.9b00034)
23.	Kazuto Ohkura, Atsushi Tabata, Yoshihiro Uto and Hitoshi Hori : Correlation Between Radiosensitizing Activity and the Stereo-structure of the TX-2036 Series of Molecules, Anticancer Research, Vol.39, No.8, 4479-4483, 2019. (要約) The stereo-configuration (R-, S-configuration) of chiral-2-nitroimidazole derivatives alters their radiosensitizing activity. This study aimed at examining the molecular features of these enantiomers by molecular simulation techniques. A series of 2-nitroimidazole-based radiosensitizer TX-2036 molecules were synthesized, and their profiles were examined using molecular structural analysis such as conformation analysis, molecular orbital analysis, and electrostatic potential analysis. R-configured TXs (TX-2043, -2030, -2036) had a weaker radiosensitizing activity than S-configured TXs (TX-2044, -2031, -2037), and R-compounds had a small minus electrostatic potential (ESP) field in the cyclopentene-1,3-dione region. S-configured TX-2046 had weaker radiosensitizing activity than R-configured TX-2045, and TX-2046 had a small minus ESP field as well as R-configured TX-2043, -2030, - 2036. The cyclopentene-1,3-dione involved in the small minus ESP field affected the radiosensitizing activity of the TX-2036 series of molecules. (キーワード) 分子設計 (molecular design) / TX-2036 / radiosensitizing activity / 構造 (structure) (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.13622 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31366548 ● Summary page in Scopus @ Elsevier: 2-s2.0-85070717728 (DOI: 10.21873/anticanres.13622, PubMed: 31366548, Elsevier: Scopus)
24.	Atsushi Tabata, Takuya Yamada, Ohtani Hiromi, Kazuto Ohkura, Toshifumi Tomoyasu and Hideaki Nagamune : β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro, Journal of Oral Microbiology, Vol.11, No.1, 1-11, 2019. (要約) Streptococcus anginosus subsp. (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines and , such as in a mouse infection model. However, no cytotoxic activity of SLS produced from β-hemolytic SAA (β-SAA) has been reported so far. In this study, the SLS-dependent cytotoxicity of the β-SAA strains including the genetically modified strains was investigated . The SLS-producing β-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. The results from this study suggest that the SLS produced from β-SAA might indicate the cytotoxic potential similar to that of the SLS from and the SLS-producing β-SAA would be recognized as "a wolf in sheep's clothing" More attention will be paid to the pathogenicity of β-hemolytic Anginosus group streptococci. (キーワード) 細菌毒素 (bacterial toxins) / 連鎖球菌 (Streptococcus) (徳島大学機関リポジトリ) ● Metadata: 113540 (出版サイトへのリンク) ● Publication site (DOI): 10.1080/20002297.2019.1609839 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31105901 ● Summary page in Scopus @ Elsevier: 2-s2.0-85065585236 (徳島大学機関リポジトリ: 113540, DOI: 10.1080/20002297.2019.1609839, PubMed: 31105901, Elsevier: Scopus)
25.	Toshifumi Tomoyasu, Keigo Tsuruno, Ryosuke Tanatsugu, Aya Miyazaki, Hiroyuki Kondo, Atsushi Tabata, Whiley A. Robert, Kenji Sonomoto and Hideaki Nagamune : Recognizability of heterologous co-chaperones with Streptococcus intermedius DnaK and Escherichia coli DnaK, Microbiology and Immunology, Vol.62, No.11, 681-693, 2018. (要約) Streptococcus intermedius DnaK complements the temperature-sensitive phenotype of an Escherichia coli dnaK null mutant only when co-chaperones DnaJ and GrpE are co-expressed. Therefore, whether S. intermedius DnaK and E. coli DnaK can recognize heterologous co-chaperones in vitro was examined. Addition of heterologous GrpE to DnaK and DnaJ partially stimulated adenosine triphosphatase (ATPase) activity, and almost completely stimulated luciferase refolding activity. Addition of heterologous DnaJ to GrpE and DnaK also stimulated ATPase activity; however, significant luciferase refolding activity was not observed. Moreover, E. coli DnaJ had a negative effect on the luciferase refolding activity of the S. intermedius DnaK chaperone system. In E. coli chaperone mutants, with the exception of E. coli DnaJ, stronger expression of the heterologous co-chaperones partially or almost completely complemented the temperature-sensitive-phenotype. These results indicate that all heterologous co-chaperones can at least partially recognize DnaK of a distantly related species. A region of the ATPase domain that is present in the DnaK of gram-negative bacteria is absent from the DnaK of gram-positive bacteria. This region is believed to be important for recognition of co-chaperones from gram-negative bacteria. However, insertion of this segment into S. intermedius DnaK failed to increase its ability to recognize E. coli co-chaperones, implying that this region is unnecessary or insufficient for the recognition of E. coli co-chaperones. Thus, our data suggest that a basic structural similarity is conserved among the components of the S. intermedius and E. coli DnaK chaperone systems, allowing weak associations between heterologous components. (キーワード) 分子シャペロン (molecular chaperone) / 大腸菌 (Escherichia coli) / 連鎖球菌 (Streptococcus) (徳島大学機関リポジトリ) ● Metadata: 115748 (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1348-0421.12651 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30239035 ● Summary page in Scopus @ Elsevier: 2-s2.0-85056121796 (徳島大学機関リポジトリ: 115748, DOI: 10.1111/1348-0421.12651, PubMed: 30239035, Elsevier: Scopus)
26.	Fumitoshi Yagishita, Chiho Nii, Yoshihiko Tezuka, Atsushi Tabata, Hideaki Nagamune, Naohiro Uemura, Yasushi Yoshida, Takashi Mino, Masami Sakamoto and Yasuhiko Kawamura : Fluorescent N-Heteroarenes Having Large Stokes Shift and Water Solubility Suitable for Bioimaging, Asian Journal of Organic Chemistry, Vol.7, No.8, 1614-1619, 2018. (出版サイトへのリンク) ● Publication site (DOI): 10.1002/ajoc.201800250 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1002/ajoc.201800250 (DOI: 10.1002/ajoc.201800250)
27.	Kazuto Ohkura, Y Kawaguchi, Y Tatematsu, Atsushi Tabata, Yoshihiro Uto and Hitoshi Hori : Structure-associated Functional Control of TX-1877 Series by Glyco-conjugation, Anticancer Research, Vol.38, No.7, 4241-4245, 2018. (要約) Sugar molecules are often used as a tool to structurally modify chemical compounds. The features of synthesized sugar-conjugated TX-1877 derivatives were herein examined. The molecular stabilities (reactivity) and hydrophobicities of sugar (e.g., monosaccharide and tetra-O-acetylated monosaccharide)-conjugated TXs were analyzed using a molecular simulation (e.g. molecular mechanics (MM) and molecular orbital (MO) analysis). The hydrophobicities of TX-1877 derivatives were increased by tetra-O-acetylation, and TX-2244 exhibited the most potent radiosensitizing activity (enhancement ratio: ER=2.30). The conformations and hydrophobicities of chemical compounds may be controlled by adding monosaccharide- and tetra-O-acetyl-conjugated sugars to TX-1877. (キーワード) 分子設計 (molecular design) / TX-1877 / structure glyco-conjugation (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.12720 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29970557 ● Summary page in Scopus @ Elsevier: 2-s2.0-85049784420 (DOI: 10.21873/anticanres.12720, PubMed: 29970557, Elsevier: Scopus)
28.	Qing Tang, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Construction of Anti-HER2 Recombinants as Targeting Modules for a Drug-delivery System Against HER2-positive Cells, Anticancer Research, Vol.38, No.7, 4319-4325, 2018. (要約) Recombinant antibodies have been investigated and used in applications such as targeting modules of drug-delivery systems (DDS) against cancers. This study aimed to prepare recombinant antibodies against HER2, containing sortase A (SrtA) recognition sequence, that are applicable as targeting modules in DDS after linkage with the drug-carrier containing oligoglycine-acceptor peptide by SrtA transpeptidation. The recombinant trastuzumab fragment antibodies (scFvs and Fab) with the SrtA-recognition motif (LPXTG) at their C-terminal were constructed and expressed in Escherichia coli and Chinese hamster ovary (CHO) cells, respectively. The reactivity of the purified recombinant antibodies towards HER2-expressing cells was also evaluated via immunofluorescent staining. Fab demonstrated higher yield and purity and better reactivity towards HER2-expressing cells (HCT-15 and HeLa) when compared to scFvs. The CHO expression system possesses superior yield and purity when compared to the E. coli expression system with respect to the preparation of recombinant antibodies applicable in targeting modules for DDS (DDS-TM). Moreover, a Fab variant prepared in this study demonstrated the potential to be a DDS-TM against HER2-expressing cancer cells. (キーワード) ドラッグデリバリーシステム (drug delivery system) / 癌治療 (cancer therapy) (出版サイトへのリンク) ● Publication site (DOI): 10.21873/anticanres.12731 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29970568 ● Summary page in Scopus @ Elsevier: 2-s2.0-85049804969 (DOI: 10.21873/anticanres.12731, PubMed: 29970568, Elsevier: Scopus)
29.	Toshifumi Tomoyasu, Masaki Matoba, Ayuko Takao, Atsushi Tabata, A Robert. Whiley, Nobuko Maeda and Hideaki Nagamune : Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene., FEMS Microbiology Letters, Vol.365, No.3, fnx-258, 2018. (要約) Streptococcus intermedius is a member of the normal human commensal flora and secretes a human-specific cytolysin intermedilysin (ILY) as a major virulence factor. Expression of ily is repressed by LacR and loss-of-function mutations of LacR are observed in many ILY high-producing strains isolated from deep-seated abscesses, suggesting that high ILY production is necessary for increased virulence. However, because ILY exhibits no β-hemolysis on animal blood agar plates, differentiating ILY high- and low-producing strains using conventional laboratory methods is not possible. Interestingly, S. intermedius also produces glycosidases, including MsgA and NanA, which exhibit N-acetyl-β-d-glucosaminidase and neuraminidase activities, respectively. Moreover, MsgA expression, but not NanA, is negatively regulated by LacR. Here we measured the activities of MsgA, NanA and ILY in strains isolated from clinical specimens and dental plaque to determine the correlation between these glycosidase activities and ILY hemolytic activity. Hemolytic activity showed a strong positive correlation with MsgA and a weak negative correlation with NanA activities. Therefore, we calculated the ratio of MsgA and NanA activity (M/N ratio). This value showed a stronger positive correlation (r = 0.81) with ILY hemolytic activity and many strains with high M/N ratios (>2) were ILY-high producers with loss-of-function mutations in LacR. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) (徳島大学機関リポジトリ) ● Metadata: 115749 (出版サイトへのリンク) ● Publication site (DOI): 10.1093/femsle/fnx258 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29228148 ● Summary page in Scopus @ Elsevier: 2-s2.0-85056596012 (徳島大学機関リポジトリ: 115749, DOI: 10.1093/femsle/fnx258, PubMed: 29228148, Elsevier: Scopus)
30.	Atsushi Tabata, Douglas Deutsch, Seiya Otsuka, Kathleen Verratti, Toshifumi Tomoyasu, Hideaki Nagamune and A. Vincent Fischetti : A novel plasmid, pSAA0430-08, from Streptococcus anginosus subsp. anginosus strain 0430-08, Plasmid, Vol.95, No.1, 16-27, 2018. (要約) Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence of MGEs in Streptococcus anginosus subsp. anginosus (SAA), a representative species belonging to AGS, and found a novel plasmid from SAA strain 0430-08. This plasmid was 7038bp and ~31% G/C content which we named pSAA0430-08, and examined its genetic structure and characteristics. Open reading frame (ORF) prediction revealed that pSAA0430-08 was composed of 10 ORFs including a putative plasmid replication protein (ORF1) and a putative toxin-antitoxin system (ORF9 and ORF10). Between ORF10 and ORF 1, four tandem repeats of 22bp each, generally termed as iteron, were also observed. Using variant plasmids of pSAA0430-08, we confirmed that both ORF1 and iteron were necessary for replication in host cells. Interestingly, the region from ORF4 to ORF7 showed homology with a genomic DNA segment of S. gordonii strains. Thus, this plasmid may travel between the different species in Streptococci, i.e., S. gordonii and S. anginosus. (キーワード) 病原微生物 (pathogenic microorganism) / プラスミド (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.plasmid.2018.01.002 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29355570 ● Summary page in Scopus @ Elsevier: 2-s2.0-85041495681 (DOI: 10.1016/j.plasmid.2018.01.002, PubMed: 29355570, Elsevier: Scopus)
31.	Tomoko Inubushi, Mayumi Sugimoto, Haruka Kunimi, Haruka Hino, Atsushi Tabata, Naohiro Imura, Shin Abe and Norio Kamemura : Biphenyl increases the intracellular Ca2+ concentration in HL-60 cells, Fundamental Toxicological Sciences, Vol.5, No.3, 99-103, 2018. (出版サイトへのリンク) ● Publication site (DOI): 10.2131/fts.5.99 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.2131/fts.5.99 (DOI: 10.2131/fts.5.99)
32.	Tatematsu Yohei, Fujita Haruhi, Hayashi Hiroki, Yamamoto Atsushi, Atsushi Tabata, Hideaki Nagamune and Ohkura Kazuto : Effects of the Nonsteroidal Anti-inflammatory Drug Celecoxib on Mitochondrial Function, Biological & Pharmaceutical Bulletin, Vol.41, No.3, 319-325, 2018. (要約) Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to treat inflammation and pain. In the present study, we examined the effects of celecoxib, a cyclooxygenase-2 (COX-2)-selective NSAID, on rat liver mitochondrial function. Celecoxib dose-dependently induced mitochondria swelling, which was not suppressed by cyclosporine A (CsA). The oxygen consumption rate in mitochondria-suspended solution was facilitated by the addition of celecoxib, and its uncoupling activity was observed. Celecoxib also suppressed SF6847-induced uncoupling, and appeared to exert inhibitory effects on the electron transport chain. Celecoxib suppressed the state 3 oxygen consumption rate in the presence of ADP. Protein release from the mitochondrial matrix was detected following the addition of celecoxib, and aldehyde dehydrogenase 2 (ALDH2) and hydroxymethylglutaryl-CoA (HMG-CoA) synthase 2 (HMGCS2) bands were confirmed in a Western blot analysis. On the other hand, protein release of cytochrome C (CytC), which is an inducer of apoptosis, from the intermembrane space was not observed. Celecoxib enhanced the membrane permeability of human erythrocytes and synthesized liposomes dose-dependently. It then induced the membrane-involving mitochondrial swelling and suppressed mitochondrial function. (キーワード) Aldehyde Dehydrogenase, Mitochondrial / Animals / Anti-Inflammatory Agents, Non-Steroidal / Celecoxib / Cell Membrane Permeability / Cyclooxygenase 2 Inhibitors / Dose-Response Relationship, Drug / Electron Transport / Erythrocytes / Hydroxymethylglutaryl-CoA Synthase / In Vitro Techniques / Male / Mitochondria / Mitochondria, Liver / Mitochondrial Swelling / Oxygen Consumption / Rats / Rats, Wistar (出版サイトへのリンク) ● Publication site (DOI): 10.1248/bpb.b17-00527 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29491208 ● Summary page in Scopus @ Elsevier: 2-s2.0-85050992357 (DOI: 10.1248/bpb.b17-00527, PubMed: 29491208, Elsevier: Scopus)
33.	Toshifumi Tomoyasu, Takahiro Yamasaki, Shinya Chiba, Shingo Kusaka, Atsushi Tabata, Robert A. Whiley and Hideaki Nagamune : Positive- and Negative-Control Pathways by Blood Components for Intermedilysin Production in Streptococcus intermedius., Infection and Immunity, Vol.85, No.9, 1-17, 2017. (要約) is an opportunistic bacterial pathogen secreting a human-specific cytolysin called intermedilysin (ILY) as a major pathogenic factor. This bacterium can degrade glycans into monosaccharides using two glycosidases, multisubstrate glycosidase A (MsgA) and neuraminidase (NanA). Here, we detected a stronger hemolytic activity mediated by ILY when PC574 was cultured in fetal bovine serum (FBS) than when it was grown in the standard culture medium. FBS-cultured cells also showed higher MsgA and NanA activity, although overproduction of ILY in FBS was undetectable in mutants -null and -null. Addition of purified MsgA and NanA to the FBS resulted in a release of 2.8 mM galactose and 4.3 mM -acetylneuraminic acid; these sugar concentrations were sufficient to upregulate the expression of ILY, MsgA, and NanA. Conversely, when strain PC574 was cultured in human plasma, no similar increase in hemolytic activity was observed. Moreover, addition of human plasma to the culture in FBS appeared to inhibit the stimulatory effect of FBS on ILY, MsgA, and NanA, although there were individual differences among the plasma samples. We confirmed that human plasma contains immunoglobulins that can neutralize ILY, MsgA, and NanA activities. In addition, human plasma had a neutralizing effect on cytotoxicity of toward HepG2 cells in FBS, and a higher concentration of human plasma was necessary to reduce the cytotoxicity of an ILY-high-producing strain than an ILY-low-producing strain. Overall, our data show that blood contains factors that stimulate and inhibit ILY expression and activity, which may affect pathogenicity of . (キーワード) Streptococcus intermedius / インターメディリシン (intermedilysin) / 細胞溶解毒素 (cytolysin) / sugar chain / 抗体 (antibody) (出版サイトへのリンク) ● Publication site (DOI): 10.1128/IAI.00379-17 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28607101 ● Summary page in Scopus @ Elsevier: 2-s2.0-85027524694 (DOI: 10.1128/IAI.00379-17, PubMed: 28607101, Elsevier: Scopus)
34.	Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo and Yoichiro Miyake : Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa., Journal of Applied Microbiology, Vol.122, No.4, 893-899, 2017. (要約) Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field due to their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen. The minimum inhibitory concentrations of 4DTBP-6,8, CPC and BAC against P. aeruginosa were measured. 4DTBP-6,8 exhibited strong antibacterial activity. We assessed the bactericidal effects of QACs against P. aeruginosa under certain conditions and their cytotoxicities in human epithelial cells using lactate dehydrogenase (LDH) release. 4DTBP-6,8 exerted excellent bactericidal effects against high concentrations of bacteria, biofilm cells and even in the presence of contaminated proteins. Cellular LDH was not released by the treatment with 4DTBP-6,8. 4DTBP-6,8 exhibited the strongest bactericidal activity against P. aeruginosa among the three QACs tested without any cytotoxicity. The potent bis-QAC, 4DTBP-6,8 has the potential to be an effective disinfectant in preventing hospital infections caused by P. aeruginosa. (キーワード) Anti-Bacterial Agents / Biofilms / Cell Line / Disinfectants / Humans / Niacinamide / 緑膿菌 (Pseudomonas aeruginosa) / Pyridinium Compounds (出版サイトへのリンク) ● Publication site (DOI): 10.1111/jam.13392 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28035713 ● Summary page in Scopus @ Elsevier: 2-s2.0-85013236835 (DOI: 10.1111/jam.13392, PubMed: 28035713, Elsevier: Scopus)
35.	Yuki Kawaguchi, Yohei Tatematsu, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Cytolytic Activity and Molecular Feature of Cardiotoxin and Cardiotoxin-like Basic Protein: The Electrostatic Potential Field Is an Important Factor for Cell Lytic Activity, Anticancer Research, Vol.35, No.8, 4515-4519, 2015. (要約) Cardiotoxin (CT) is a well-known cell lytic protein and has been purified from cobra venom. Cardiotoxin-like basic protein (CLBP) has two amino acid insertions and does not exhibit cell lytic activity. The molecular features of these CT family proteins were examined in the present study using molecular modeling and molecular simulation techniques. Molecular models of CT and CLBP were constructed based on the X-ray data of Naja mossambica mossambica CT VII4 (Protein Data Bank ID: 1CDT). The structural features of these models were examined using molecular orbital and electrostatic potential parameters. The stereo-hydrophobicities and molecular torsions of CT and CLBP, which are indexes of structural features, were similar. Electrostatic potential fields (ESP) differed between CT and CLBP and this was considered one of the critical factors in molecular titer. The distribution of ESP fields may affect the cytolytic activity of the CT family. (キーワード) Amino Acid Sequence / Animals / Cobra Cardiotoxin Proteins / Elapidae / Hydrophobic and Hydrophilic Interactions / Models, Molecular / Molecular Sequence Data / Protein Conformation / Sequence Alignment / Static Electricity (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26168495 ● Summary page in Scopus @ Elsevier: 2-s2.0-84939786315 (PubMed: 26168495, Elsevier: Scopus)
36.	Atsushi Tabata, Ohkubo Yukihisa, Natsuki Anyhoji, Keiko Hojo, Toshifumi Tomoyasu, Youhei Tatematsu, Kazuto Ohkura and Hideaki Nagamune : Development of a Sortase A-mediated Peptide-labeled Liposome Applicable to Drug-delivery Systems, Anticancer Research, Vol.35, No.8, 4411-4417, 2015. (要約) In order to develop an efficient drug-delivery system (DDS), a lipopeptide-loaded liposome that functions as a platform for the transpeptidase reaction mediated by sortase A (SrtA) was constructed and its stability, as well as cell-specific targeting were evaluated in the present study. Several lipopeptides possessing an acceptor peptide sequence (oligoglycine ≥ three residues) or donor peptide sequence (LPETG) for the SrtA-mediated reaction were chemically synthesized and then inserted into the liposome membrane composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (DPPC-Chol-lipo) to obtain the lipopeptide-loaded liposomes. The transpeptidase reaction mediated by recombinant SrtA (His-ΔN59SrtA) was employed to modify the peptide moiety on the liposomal surface using a fluorescently-labeled substrate peptide corresponding to the species of each loaded lipopeptide. Furthermore, lung tumor-binding peptide (LTBP)-labeled liposomes, prepared by this transpeptidase reaction, were investigated for selective targeting to lung cancer cells in vitro. The His-ΔN59SrtA-mediated transpeptidation of fluorescently-labeled peptide on the lipopeptide-loaded DPPC-Chol-lipo was confirmed. The selective targeting of LTBP-labeled liposomes to the lung cancer cell line A549 was also observed in vitro. These results suggest that the labeling of acceptor or donor lipopeptide-loaded liposomes with the transpeptidase SrtA could be a useful method for developing a platform applicable to a cancer-targeting DDS. (キーワード) ドラッグデリバリーシステム (drug delivery system) / リポソーム (liposomes) (徳島大学機関リポジトリ) ● Metadata: 117781 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26168480 ● Summary page in Scopus @ Elsevier: 2-s2.0-84939824857 (徳島大学機関リポジトリ: 117781, PubMed: 26168480, Elsevier: Scopus)
37.	Onodera Takefumi, Kuriyama Isoko, Sakamoto Yuka, Kawamura Moe, Kuramochi Kouji, Tsubaki Kazunori, Atsushi Tabata, Hideaki Nagamune and Mizushina Yoshiyuki : 5-O-Acyl plumbagins inhibit DNA polymerase activity and suppress the inflammatory response, Archives of Biochemistry and Biophysics, Vol.573, 100-110, 2015. (要約) We previously found that vitamin K3 (menadione, 2-methyl-1,4-naphthoquinone) inhibits the activity of human mitochondrial DNA polymerase (pol) γ. In this study, we focused on plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), and chemically synthesized novel plumbagins conjugated with C2:0 to C22:6 fatty acids (5-O-acyl plumbagins). These chemically modified plumbagins displayed enhanced mammalian pol inhibition, with plumbagin conjugated to docosahexaenoic acid (C22:6-acyl plumbagin) exhibiting the strongest inhibition of pol λ among the ten 5-O-acyl plumbagins synthesized. C22:6-acyl plumbagin selectively inhibited the activities of mammalian pol species, but did not influence the activities of other pols or DNA metabolic enzymes tested. The inhibition of pol λ, a DNA repair/recombination pol, by these compounds was significantly correlated with both their suppression of lipopolysaccharide (LPS) induced tumor necrosis factor-α (TNF-α) production by mouse RAW264.7 macrophages and the reduction of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in the mouse ear. These data indicate that 5-O-acyl plumbagins act as anti-inflammatory agents by inhibiting mammalian pol λ. These results further suggest that C22:6-acyl plumbagin is a promising anti-inflammatory candidate and that acylation could be an effective chemical modification to improve the anti-inflammatory activity of vitamin K3 derivatives, such as plumbagin. (キーワード) Animals / Anti-Inflammatory Agents / Cell Line / DNA Polymerase beta / Escherichia coli Proteins / Fatty Acids / Gene Knockdown Techniques / Humans / Inflammation / Lipopolysaccharides / Macrophages / Mice / Naphthoquinones / Plant Proteins / Rats / Tetradecanoylphorbol Acetate / Tumor Necrosis Factor-alpha (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.abb.2015.02.032 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25748000 ● Summary page in Scopus @ Elsevier: 2-s2.0-84926219634 (DOI: 10.1016/j.abb.2015.02.032, PubMed: 25748000, Elsevier: Scopus)
38.	Kim Hyejin, Atsushi Tabata, Toshifumi Tomoyasu, Ueno Tomomi, Uchiyama Shigeto, Keizo Yuasa, Akihiko Tsuji and Hideaki Nagamune : Estrogen stimuli promote osteoblastic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells., Journal of Bone and Mineral Metabolism, Vol.33, No.1, 30-39, 2015. (要約) Estrogenic compounds include endogenous estrogens such as estradiol as well as soybean isoflavones, such as daidzein and its metabolite equol, which are known phytoestrogens that prevent osteoporosis in postmenopausal women. Indeed, mineralization of MC3T3-E1 cells, a murine osteoblastic cell line, was significantly decreased in medium containing fetal bovine serum treated with charcoal-dextran to deplete endogenous estrogens, but estradiol and these soybean isoflavones dose-dependently restored the differentiation of MC3T3-E1 cells; equal was tenfold more effective than daidzein. These differentiation promoting effects were inhibited by the addition of fulvestrant, which is a selective downregulator of estrogen receptors. Analysis of the expression pattern of bone-related genes by reverse transcription PCR (RT-PCR)/quantitative real-time PCR (qRT-PCR), which focused on responsiveness to the estrogen stimuli, revealed that the transcription of PACE4, a subtilisin-like proprotein convertase, was tightly linked with the differentiation of MC3T3-E1 cells induced by estrogen stimuli. Moreover, treatment with RNAi of PACE4 in MC3T3-E1 cells resulted in a drastic decrease of mineralization in the presence of estrogen stimuli. These results strongly suggest that PACE4 participates in bone formation at least in osteoblast differentiation, and estrogen receptor-mediated stimuli induce osteoblast differentiation through the upregulation of PACE4 expression. (キーワード) 骨芽細胞 (osteoblast) / PACE4 / Estrogen / Soybean / isoflavone (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s00774-014-0567-9 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24557631 ● Summary page in Scopus @ Elsevier: 2-s2.0-84893948353 (DOI: 10.1007/s00774-014-0567-9, PubMed: 24557631, Elsevier: Scopus)
39.	Kawaguchi Yuki, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Molecular Analysis of Streptococcus anginosus-derived SagA Peptides, Anticancer Research, Vol.34, No.8, 4627-4631, 2014. (要約) SagA1 and SagA2 molecules produced from beta-hemolytic Streptococcus anginosus subsp. anginosus are composed of a leader peptide and a propeptide, and their mature form has hemolytic activity as a well-known Streptococcal peptide toxin, streptolysin. The function of these SagA molecules is thought to be dependent on intra-molecular heterocycle formation. In this study, we examined the heterocycle-involved molecular features of SagA1, SagA2, and S. pyogenes SagA (SPySagA), focusing on their heterocycle formation. Molecular models of SagA1, SagA2, and SPySagA were constructed using a molecular modeling technique. Molecular dynamics and molecular mechanic analyses of the modeled SagA molecules were performed to obtain their energy profiles. Total energy of the modeled SagA1, SagA2, and SPySagA decreased with heterocycle formation, and the border between the leader peptide and propeptide was clearly observed after heterocycle formation. The flexibility of SagA molecules was changed by intramolecular heterocycle formation, and their function (e.g. hemolytic activity) seems to be regulated by structural transition with heterocycle formation. (キーワード) SagA / Streptolysin S / Streptococcus anginosus (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25075110 ● Summary page in Scopus @ Elsevier: 2-s2.0-84908662068 (PubMed: 25075110, Elsevier: Scopus)
40.	Atsushi Tabata, Natsuki Anyoji, Yukimasa Ohkubo, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation on the Reaction Conditions of Staphylococcus aureus Sortase A for Creating Surface-modified Liposomes as a Drug-delivery System Tool, Anticancer Research, Vol.34, No.8, 4521-4527, 2014. (要約) This study aimed to determine the preferred conditions for the transpeptidase reaction of sortase A from Staphylococcus aureus, for the purpose of creating functional liposomes useful for a drug-delivery system (DDS). His-tagged recombinant sortase A with 59 amino acids deleted from the N-terminus (His-ΔN59SrtA) was prepared using an Escherichia coli expression system. The pH dependency and sorting signal sequence dependency of the transpeptidase reaction of His-ΔN59SrtA were analyzed by monitoring the transfer of model donor-substrates (i.e. His-tagged mutant green fluorescent proteins with a C-terminal LPxTG sorting signal) to model acceptor-beads with a GGGGGC peptide. In addition, using preferred conditions, the sortase A reaction was used to modify liposome surface. The transpeptidase reaction of His-ΔN59SrtA was enhanced under weakly acidic conditions. Transfer efficiency, based on sorting signal recognition by His-ΔN59SrtA, was similar to or higher than that obtained using several substrates with amino acids other than Glu in the sorting signal position "x". Furthermore, liposomes containing GGGGGC peptide-linked dipalmitoylphosphatidylethanolamine were successfully modified using the preferred conditions for His-ΔN59SrtA determined in this study. Preferred conditions for the transpeptidase reaction of His-ΔN59SrtA, especially in a weakly acidic environment to enhance reaction, was established and successfully used to create functional liposomes applicable to DDS. (キーワード) Sortase A (徳島大学機関リポジトリ) ● Metadata: 117782 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25075095 ● Summary page in Scopus @ Elsevier: 2-s2.0-84908660909 (徳島大学機関リポジトリ: 117782, PubMed: 25075095, Elsevier: Scopus)
41.	Hidenori Imaki, Toshifumi Tomoyasu, Naoki Yamamoto, Chiharu Taue, Sachiko Masuda, Ayuko Takao, Nobuko Maeda, Atsushi Tabata, Robert A. Whiley and Hideaki Nagamune : Identification and Characterization of a Novel Secreted Glycosidase with Multiple Glycosidase Activities in Streptococcus intermedius, Journal of Bacteriology, Vol.196, No.15, 2817-2826, 2014. (要約) Streptococcus intermedius is a known human pathogen and belongs to the anginosus group (S. anginosus, S. intermedius, and S. constellatus) of streptococci (AGS). We found a large open reading frame (6,708 bp) in the lac operon, and bioinformatic analysis suggested that this gene encodes a novel glycosidase that can exhibit β-d-galactosidase and N-acetyl-β-d-hexosaminidase activities. We, therefore, named this protein "multisubstrate glycosidase A" (MsgA). To test whether MsgA has these glycosidase activities, the msgA gene was disrupted in S. intermedius. The msgA-deficient mutant no longer showed cell- and supernatant-associated β-d-galactosidase, β-d-fucosidase, N-acetyl-β-d-glucosaminidase, and N-acetyl-β-d-galactosaminidase activities, and all phenotypes were complemented in trans with a recombinant plasmid carrying msgA. Purified MsgA had all four of these glycosidase activities and exhibited the lowest Km with 4-methylumbelliferyl-linked N-acetyl-β-d-glucosaminide and the highest kcat with 4-methylumbelliferyl-linked β-d-galactopyranoside. In addition, the purified LacZ domain of MsgA had β-d-galactosidase and β-d-fucosidase activities, and the GH20 domain exhibited both N-acetyl-β-d-glucosaminidase and N-acetyl-β-d-galactosaminidase activities. The β-d-galactosidase and β-d-fucosidase activities of MsgA are thermolabile, and the optimal temperature of the reaction was 40°C, whereas almost all enzymatic activities disappeared at 49°C. The optimal temperatures for the N-acetyl-β-d-glucosaminidase and N-acetyl-β-d-galactosaminidase activities were 58 and 55°C, respectively. The requirement of sialidase treatment to remove sialic acid residues of the glycan branch end for glycan degradation by MsgA on human α1-antitrypsin indicates that MsgA has exoglycosidase activities. MsgA and sialidase might have an important function in the production and utilization of monosaccharides from oligosaccharides, such as glycans for survival in a normal habitat and for pathogenicity of S. intermedius. (キーワード) Streptococcus intermedius / glycosidase / multi-substrates glycosidase (出版サイトへのリンク) ● Publication site (DOI): 10.1128/JB.01727-14 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24858187 ● Summary page in Scopus @ Elsevier: 2-s2.0-84903904703 (DOI: 10.1128/JB.01727-14, PubMed: 24858187, Elsevier: Scopus)
42.	Atsushi Tabata, Yuji Sato, Kentaro Maya, Kota Nakano, Ken Kikuchi, Robert A. Whiley, Kazuto Ohkura, Toshifumi Tomoyasu and Hideaki Nagamune : A streptolysin S homologue is essential for beta-haemolytic Streptococcus constellatus subsp. constellatus cytotoxicit, Microbiology, Vol.160, No.5, 980-991, 2014. (要約) Streptococcus constellatus is a member of the Anginosus group streptococci (AGS) and primarily inhabits the human oral cavity. S. constellatus is composed of three subspecies: S. constellatus subsp. constellatus (SCC), S. constellatus subsp. pharyngis and the newly described subspecies S. constellatus subsp. viborgensis. Although previous studies have established that SCC contains β-haemolytic strains, the factor(s) responsible for β-haemolysis in β-haemolytic SCC (β-SCC) has yet to be clarified. Recently, we discovered that a streptolysin S (SLS) homologue is the β-haemolytic factor of β-haemolytic Streptococcus anginosus subsp. anginosus (β-SAA), another member of the AGS. Furthermore, because previous studies have suggested that other AGS species, except for Streptococcus intermedius, do not possess a haemolysin(s) belonging to the family of cholesterol-dependent cytolysins, we hypothesized that, as with β-SAA, the SLS homologue is the β-haemolytic factor of β-SCC, and therefore aimed to investigate and characterize the haemolytic factor of β-SCC in the present study. PCR amplification revealed that all of the tested β-SCC strains were positive for the sagA homologue of SCC (sagA(SCC)). Further investigations using β-SCC strain W277 were conducted to elucidate the relationship between sagA(SCC) and β-haemolysis by constructing sagA(SCC) deletion mutants, which completely lost β-haemolytic activity. This loss of β-haemolytic activity was restored by trans-complementation of sagA(SCC). Furthermore, a co-cultivation assay established that the cytotoxicity of β-SCC was clearly dependent on the presence of sagA(SCC). These results demonstrate that sagA(SCC) is the factor responsible for β-SCC β-haemolysis and cytotoxicity. (キーワード) Streptolysin S / Streptococcus constellatus / beta-hemolytic / 細胞毒性 (cytotoxicity) (出版サイトへのリンク) ● Publication site (DOI): 10.1099/mic.0.075580-0 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24600025 ● Search Scopus @ Elsevier (PMID): 24600025 ● Search Scopus @ Elsevier (DOI): 10.1099/mic.0.075580-0 (DOI: 10.1099/mic.0.075580-0, PubMed: 24600025)
43.	Atsushi Tabata, Kazuto Ohkura, Ohkubo Yukimasa, Toshifumi Tomoyasu, Ohkuni Hisashi, Whiley A. Robert and Hideaki Nagamune : The diversity of receptor recognition in cholesterol-dependent cytolysins, Microbiology and Immunology, Vol.58, No.3, 155-171, 2014. (要約) Cholesterol-dependent cytolysins (CDCs) are bacterial pore-forming toxins secreted mainly by pathogenic Gram-positive bacteria. CDCs generally recognize and bind to membrane cholesterol to create pores and lyse target cells. However, in contrast to typical CDCs such as streptolysin O, several atypical CDCs have been reported. The first of these was intermedilysin, which is secreted by Streptococcus intermedius and has human cell-specificity, human CD59 (huCD59) being its receptor. In the study reported here, the diversity of receptor recognition among CDCs was investigated and multi-receptor recognition characteristics were identified within this toxin family. Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) secreted by S. mitis strain Nm-65 isolated from a patient with Kawasaki disease was previously shown to hemolyze erythrocytes in a species-dependent manner, its maximum activity being in human cells. In the present study, it was found that Sm-hPAF recognizes both membrane cholesterol and huCD59 as receptors for triggering pore-formation. Moreover, vaginolysin (VLY) of Gardnerella vaginalis showed similar characteristics to Sm-hPAF regarding receptor recognition. On the basis of the results presented here, the mode of receptor recognition of CDCs can be categorized into the following three groups: (i) Group I, comprising typical CDCs with high affinity to cholesterol and no or very little affinity to huCD59; (ii) Group II, including atypical CDCs such as ILY, with no or very little affinity to cholesterol and high affinity to huCD59; and (iii) Group III, which contains atypical CDCs such as Sm-hPAF and VLY with affinity to both cholesterol and huCD59. (キーワード) 細胞溶解毒素 (cytolysin) / cholesterol-dependet cytolysin / diversity (出版サイトへのリンク) ● Publication site (DOI): 10.1111/1348-0421.12131 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24401114 ● CiNii @ 国立情報学研究所 (CRID): 1520291853530158464 ● Summary page in Scopus @ Elsevier: 2-s2.0-84895562361 (DOI: 10.1111/1348-0421.12131, PubMed: 24401114, CiNii: 1520291853530158464, Elsevier: Scopus)
44.	Toshifumi Tomoyasu, Hidenori Imaki, Sachiko Masuda, Ayumi Okamoto, HyeJin Kim, Richard Waite, Robert Whiley, Ken Kikuchi, Keiichi Hiramatsu, Atsushi Tabata and Hideaki Nagamune : LacR mutations are frequently observed in Streptococcus intermedius and are responsible for increased intermedilysin production and virulence., Infection and Immunity, Vol.81, No.9, 3276-3286, 2013. (要約) Streptococcus intermedius secretes a human-specific cytolysin, intermedilysin (ILY), which is considered to be the major virulence factor of this pathogen. We screened for a repressor of ily expression by using random gene disruption in a low-ILY-producing strain (PC574). Three independent high-ILY-producing colonies that had plasmid insertions within a gene that has high homology to lacR were isolated. Validation of these observations was achieved through disruption of lacR in strain PC574 with an erythromycin cassette, which also led to higher hemolytic activity, increased transcription of ily, and higher cytotoxicity against HepG2 cells, compared to the parental strain. The direct binding of LacR within the ily promoter region was shown by a biotinylated DNA probe pulldown assay, and the amount of ILY secreted into the culture supernatant by PC574 cells was increased by adding lactose or galactose to the medium as a carbon source. Furthermore, we examined lacR nucleotide sequences and the hemolytic activity of 50 strains isolated from clinical infections and 7 strains isolated from dental plaque. Of the 50 strains isolated from infections, 13 showed high ILY production, 11 of these 13 strains had one or more point mutations and/or an insertion mutation in LacR, and almost all mutations were associated with a marked decline in LacR function. These results strongly suggest that mutation in lacR is required for the overproduction of ILY, which is associated with an increase in pathogenicity of S. intermedius. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) / 遺伝子発現 (gene expression) (出版サイトへのリンク) ● Publication site (DOI): 10.1128/IAI.00638-13 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23798532 ● Summary page in Scopus @ Elsevier: 2-s2.0-84884217618 (DOI: 10.1128/IAI.00638-13, PubMed: 23798532, Elsevier: Scopus)
45.	Kawaguchi Yuki, Atsushi Tabata, Hideaki Nagamune and Kazuto Ohkura : Profiles of ILY, VLY and Sm-hPAF interaction with human CD59, Anticancer Research, Vol.33, No.7, 2901-2904, 2013. (要約) The molecular features of a new member of the bacterially -derived cytolysin family were examined. In particular, the interactive mechanisms of intermedilysin (ILY), vaginolysin (VLY), and Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) with human CD59 (hCD59) were analyzed. Molecular models of VLY and Sm-hPAF were constructed based on X-ray data of ILY (protein data bank ID=1S3R), and their interactive profiles with hCD59 were examined using molecular simulation. Non-binding (NB) energy between ILY and hCD59 was three orders of magnitude higher than the energy between VLY and hCD59. NB energy between Sm-hPAF and hCD59 was similar to that between VLY and hCD59. A hydrogen bond (ILY Arg432-hCD59 Glu76) was observed between ILY and hCD59, and a stronger interaction was formed by flexible adjustment between them. (キーワード) 細胞溶解毒素 (cytolysin) / コレステロール (cholesterol) / human-specific / インターメディリシン (intermedilysin) / CD59 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23780977 ● Search Scopus @ Elsevier (PMID): 23780977 (PubMed: 23780977)
46.	Atsushi Tabata, Yukimasa Ohkubo, Masato Tamura, Toshifumi Tomoyasu, Kazuto Ohkura and Hideaki Nagamune : Construction of an improved drug delivery system tool with enhanced versatility in cell-targeting., Anticancer Research, Vol.33, No.7, 2905-2910, 2013. (要約) His-Z-CDC(ss)(IS) was revealed to be an improved DDS tool with enhanced versatility in cell targeting. (キーワード) Carcinoembryonic Antigen / Cell Membrane / Cells, Cultured / コレステロール (cholesterol) / Drug Delivery Systems / Fibroblasts / Fluorouracil / Hemolysis / Hep G2 Cells / Humans / Immunoglobulin G / リポソーム (liposomes) / Perforin / Protein Transport / Recombinant Fusion Proteins / Staphylococcus aureus (徳島大学機関リポジトリ) ● Metadata: 117783 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23780978 ● Search Scopus @ Elsevier (PMID): 23780978 (徳島大学機関リポジトリ: 117783, PubMed: 23780978)
47.	Atsushi Tabata, Kota Nakano, Kazuto Ohkura, Toshifumi Tomoyasu, Ken Kikuchi, Robert Whiley and Hideaki Nagamune : Novel Twin Streptolysin S-Like Peptides Encoded in the sag Operon Homologue of Beta-Hemolytic Streptococcus anginosus., Journal of Bacteriology, Vol.195, No.5, 1090-1099, 2013. (要約) Streptococcus anginosus is a member of the anginosus group streptococci, which form part of the normal human oral flora. In contrast to the pyogenic group streptococci, our knowledge of the virulence factors of the anginosus group streptococci, including S. anginosus, is not sufficient to allow a clear understanding of the basis of their pathogenicity. Generally, hemolysins are thought to be important virulence factors in streptococcal infections. In the present study, a sag operon homologue was shown to be responsible for beta-hemolysis in S. anginosus strains by random gene knockout. Interestingly, contrary to pyogenic group streptococci, beta-hemolytic S. anginosus was shown to have two tandem sagA homologues, encoding streptolysin S (SLS)-like peptides, in the sag operon homologue. Gene deletion and complementation experiments revealed that both genes were functional, and these SLS-like peptides were essential for beta-hemolysis in beta-hemolytic S. anginosus. Furthermore, the amino acid sequence of these SLS-like peptides differed from that of the typical SLS of S. pyogenes, especially in their propeptide domain, and an amino acid residue indicated to be important for the cytolytic activity of SLS in S. pyogenes was deleted in both S. anginosus homologues. These data suggest that SLS-like peptides encoded by two sagA homologues in beta-hemolytic S. anginosus may be potential virulence factors with a different structure essential for hemolytic activity and/or the maturation process compared to the typical SLS present in pyogenic group streptococci. (キーワード) 連鎖球菌 (Streptococcus) / Streptolysin S (徳島大学機関リポジトリ) ● Metadata: 117785 (出版サイトへのリンク) ● Publication site (DOI): 10.1128/JB.01344-12 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23292771 ● Search Scopus @ Elsevier (PMID): 23292771 ● Search Scopus @ Elsevier (DOI): 10.1128/JB.01344-12 (徳島大学機関リポジトリ: 117785, DOI: 10.1128/JB.01344-12, PubMed: 23292771)
48.	Toshifumi Tomoyasu, Atsushi Tabata, Yoko Ishikawa, Robert Whiley and Hideaki Nagamune : Small heat shock protein AgsA: an effective stabilizer of enzyme activities., Journal of Bioscience and Bioengineering, Vol.115, No.1, 15-19, 2013. (要約) A small heat shock protein, AgsA, possesses chaperone activity that can reduce the amount of heat-aggregated protein in vivo, and suppress the aggregation of chemical- and heat-denatured proteins in vitro. Therefore, we examined the ability of AgsA to stabilize the activity of several enzymes by using this chaperone activity. We observed that AgsA can stabilize the enzymatic activities of Renilla (Renilla reniformis) luciferase, firefly (Photinus pyralis) luciferase, and β-galactosidase, and showed comparable or greater stabilization of these enzymes than bovine serum albumin (BSA), a well-known stabilizer of enzyme activities. In particular, AgsA revealed better stabilization of Renilla luciferase and β-galactosidase than BSA under disulfide bond-reducing conditions with dithiothreitol. In addition, AgsA also increased the enzymatic performance of β-galactosidase and various restriction enzymes to a comparable or greater extent than BSA. These data indicate that AgsA may be useful as a general stabilizer of enzyme activities. (キーワード) Small heat shock protein / HSP / 酵素 (enzyme) (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.jbiosc.2012.08.001 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22929984 ● Search Scopus @ Elsevier (PMID): 22929984 ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbiosc.2012.08.001 (DOI: 10.1016/j.jbiosc.2012.08.001, PubMed: 22929984)
49.	Kazuto Ohkura, Yuki Kawaguchi, Atsushi Tabata, Atsushi Yamamoto, Yasuo Shinohara, Hideaki Nagamune and Hitoshi Hori : Molecular Profiles of Cholesterol-dependent Cytolysin Family-derived 11mer Regions, Anticancer Research, Vol.32, No.6, 2343-2346, 2012. (要約) Cholesterol-dependent cytolysins (CDCs) are secreted from various types of bacteria and are involved in various diseases (e.g. abscess formation). Traditional CDCs has a conserved 11mer region, which is a key structure in membrane recognition. Based on the X-ray data of traditional CDC perfringolysin O (PFO), molecular models of intermedilysin (ILY), pyolysin (PLO), vaginolysin (VLY), and Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) were constructed. The 11mer regions of these models were extracted, and their molecular features were analyzed. The dipole moments of these 11mer regions were classified into four types, and their stereo-hydrophobicity (dGW) was different. It was thought that these results influenced the species specificity and membrane recognition of each cytolysin. Traditional CDCs, ILY, PLO, and VLY consisted of four domains (domains 1 to 4). Domain 0 existed on the N-terminal side in Sm-hPAF in addition to these four domains. The 11mer sequence of Sm-hPAF is the same as that of VLY, but Sm-hPAF has slightly different characteristics (e.g. species specificity, membrane recognition, cholesterol dependency) compared to VLY. Dynamic structure analysis of domain 0 might clarify these differences. (キーワード) Amino Acid Sequence / Cytotoxins / Humans / Models, Molecular / Molecular Sequence Data / X-Ray Diffraction (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22641672 ● Search Scopus @ Elsevier (PMID): 22641672 (PubMed: 22641672)
50.	Atsushi Tabata, Yukimasa Ohkubo, Eriko Sakakura, Toshifumi Tomoyasu, Kazuto Ohkura and Hideaki Nagamune : Investigation of a Bacterial Pore-forming Chimera Toxin for Application as a Novel Drug-delivery System Tool, Anticancer Research, Vol.32, No.6, 2323-2330, 2012. (要約) Cholesterol-dependent cytolysins (CDCs) are pore-forming toxins from Gram-positive bacteria. The aim of this study was to investigate the potential of a CDC, intermedilysin, as a drug-delivery system (DDS) for clinical application. Intermedilysin was modified by the addition of a disulfide bridge to regulate pore formation, by swapping domain 4 to provide cholesterol-binding capacity, and by the introduction of a targeting domain. The resultant chimera protein, His-LTBP-CDC(ss)(IP), was investigated for its use as a DDS tool in vitro. His-LTBP-CDC(ss)(IP) exhibited a regulated pore-forming capacity under reducing conditions. This chimera protein was able to deliver a drug-carrier liposome specifically to the target cell, to be endocytosed into the cell with subsequent release of the components into the cytoplasm. A chimera protein derived from the bacterial pore-forming toxin intermedilysin (His-LTBP-CDC(ss)(IP)) forms the basis for a novel DDS tool. (キーワード) Bacteriocins / Cell Line, Tumor / Drug Delivery Systems / Electrophoresis, Polyacrylamide Gel / Humans / Liposomes / Microscopy, Fluorescence / Recombinant Fusion Proteins (徳島大学機関リポジトリ) ● Metadata: 117784 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22641669 ● Summary page in Scopus @ Elsevier: 2-s2.0-84864557140 (徳島大学機関リポジトリ: 117784, PubMed: 22641669, Elsevier: Scopus)
51.	Toshifumi Tomoyasu, Atsushi Tabata, Hidenori Imaki, Keigo Tsuruno, Aya Miyazaki, Kenji Sonomoto, Robert Whiley and Hideaki Nagamune : Role of Streptococcus intermedius DnaK chaperone system in stress tolerance and pathogenicity, Cell Stress & Chaperones, Vol.17, No.1, 41-55, 2012. (要約) Streptococcus intermedius is a facultatively anaerobic, opportunistic pathogen that causes purulent infections and abscess formation. The DnaK chaperone system has been characterized in several pathogenic bacteria and seems to have important functions in stress resistance and pathogenicity. However, the role of DnaK in S. intermedius remains unclear. Therefore, we constructed a dnaK knockout mutant that exhibited slow growth, thermosensitivity, accumulation of GroEL in the cell, and reduced cytotoxicity to HepG2 cells. The level of secretion of a major pathogenic factor, intermedilysin, was not affected by dnaK mutation. We further examined the function and property of the S. intermedius DnaK chaperone system by using Escherichia coli ΔdnaK and ΔrpoH mutant strains. S. intermedius DnaK could not complement the thermosensitivity of E. coli ΔdnaK mutant. However, the intact S. intermedius DnaK chaperone system could complement the thermosensitivity and acid sensitivity of E. coli ΔdnaK mutant. The S. intermedius DnaK chaperone system could regulate the activity and stability of the heat shock transcription factor ς(32) in E. coli, although S. intermedius does not utilize ς(32) for heat shock transcription. The S. intermedius DnaK chaperone system was also able to efficiently eliminate the aggregated proteins from ΔrpoH mutant cells. Overall, our data showed that the S. intermedius DnaK chaperone system has important functions in quality control of cellular proteins but has less participation in the modulation of expression of pathogenic factors. (キーワード) 連鎖球菌 (Streptococci) / 分子シャペロン (molecular chaperone) / 病原性 (出版サイトへのリンク) ● Publication site (DOI): 10.1007/s12192-011-0284-4 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21822788 ● Search Scopus @ Elsevier (PMID): 21822788 ● Search Scopus @ Elsevier (DOI): 10.1007/s12192-011-0284-4 (DOI: 10.1007/s12192-011-0284-4, PubMed: 21822788)
52.	Hisashi Ohkuni, Hideaki Nagamune, Nana Ozaki, Atsushi Tabata, Yuko Todome, Yukino Watanabe, Hidemi Takahashi, Kazuto Ohkura, Hiroki Kourai, Hiromi Ohtsuka, Vincent A. Fischetti and John B. Zabriskie : Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor, APMIS, Vol.120, No.1, 56-71, 2012. (要約) We previously purified Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) from the culture supernatant of S. mitis strain Nm-65, isolated from the tooth surface of a patient with Kawasaki disease. Here we produced recombinant Sm-hPAF protein (rSm-hPAF) in Escherichia coli, to determine whether rSm-hPAF conserves its platelet aggregation activity. rSm-hPAF precursor (665 amino acids) shows up to 36-56% identity with the family of cholesterol-dependent cytolysins (CDCs), and rSm-hPAF displayed potent hemolytic activity toward mammalian erythrocytes, including human erythrocytes with platelet aggregation activity. The 162-amino acid amino-terminal domain of rSm-hPAF was found in no other CDCs except lectinolysin; this domain is homologous to a portion of pneumococcal fucolectin-related protein. Interestingly, suilysin (SLY) and pneumolysin (PLY) of CDCs also exhibit substantial human platelet aggregation activity, similar to rSm-hPAF, and the platelet aggregation by rSm-hPAF, SLY, and PLY was morphologically confirmed using light and electron microscopy. (キーワード) Adult / Amino Acid Sequence / Animals / Antibodies, Monoclonal / Bacterial Proteins / 細菌毒素 (bacterial toxins) / Base Sequence / Blood Platelets / Cell Adhesion Molecules / コレステロール (cholesterol) / Cytotoxins / DNA, Bacterial / Female / Genes, Bacterial / Hemolysin Proteins / Humans / L-Lactate Dehydrogenase / Mice / Mice, Inbred BALB C / 透過電子顕微鏡法 (transmission electron microscopy) / Molecular Sequence Data / Mucocutaneous Lymph Node Syndrome / Phylogeny / Platelet Aggregation / Recombinant Proteins / Sequence Homology, Amino Acid / Streptococcus mitis / Streptolysins (出版サイトへのリンク) ● Publication site (DOI): 10.1111/j.1600-0463.2011.02813.x (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22151309 ● Search Scopus @ Elsevier (PMID): 22151309 ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0463.2011.02813.x (DOI: 10.1111/j.1600-0463.2011.02813.x, PubMed: 22151309)
53.	Heni Susilowati, Hirohiko Okamura, Katsuhiko Hirota, Masayuki Shono, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells., Biochemical and Biophysical Research Communications, Vol.404, No.1, 57-61, 2011. (要約) (Intermedilysin (ILY) is a cholesterol-dependent cytolysin produced by Streptococcus intermedius, which is associated with human brain and liver abscesses. Although intrahepatic bile duct cells play a valuable role in the pathogenesis of liver abscess, the molecular mechanism of ILY-treated intrahepatic bile duct cells remains unknown. In this study, we report that ILY induced a nuclear accumulation of intracellular calcium ([Ca(2+)]i) in human cholangiocellular cells HuCCT1. We also demonstrate that 10 ng/ml ILY induced NFAT1 dephosphorylation and its nuclear translocation in HuCCT1 cells. In contrast to the result that ILY induced NF-B translocation in human hepatic HepG2 cells, ILY did not affect NF-B localization in HuCCT1 cells. Dephosphorylation and nuclear translocation of NFAT1 caused by ILY were prevented by [Ca(2+)]i calcium chelator, BAPTA/AM, and calcineurin inhibitors, cyclosporine A and tacrolimus. ILY induced early growth response-1 (EGR-1) expression and it was inhibited by the pre-treatment with cyclosporine A, indicating that the calcineurin/NFAT pathway was involved in EGR-1 expression in response to ILY. ILY-induced calcineurin/NFAT1 activation and sequential EGR-1 expression might be related to the pathogenesis of S. intermedius in human bile duct cells.) (キーワード) Bacteriocins / Bile Ducts, Intrahepatic / Calcineurin / カルシウム (calcium) / Cell Line, Tumor / Cell Nucleus / Early Growth Response Protein 1 / Humans / NFATC Transcription Factors (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbrc.2010.11.057 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21094139 ● Summary page in Scopus @ Elsevier: 2-s2.0-78650872240 (DOI: 10.1016/j.bbrc.2010.11.057, PubMed: 21094139, Elsevier: Scopus)
54.	Toshifumi Tomoyasu, Atsushi Tabata, Hiroshima Riki, Imaki Hidenori, Masuda Sachiko, Whiley A. Robert, Aduse-Opoku Joseph, Kikuchi Ken, Hiramatsu Keiichi and Hideaki Nagamune : Role of catabolite control protein A in the regulation of intermedilysin production by Streptococcus intermedius., Infection and Immunity, Vol.78, No.9, 4012-4021, 2010. (要約) Streptococcus intermedius is an opportunistic pathogen of humans that causes purulent infections, including brain and liver abscesses. This pathogen secretes a human-specific cytolysin, intermedilysin, which has been recognized as a major virulence factor. However, most of the expressional control mechanisms of ily are still unknown. To determine these mechanisms, we analyzed the nucleotide sequence of the ily promoter region. We found a highly homologous region to the catabolite-repressible element (cre) in the ily promoter region and observed a considerable decrease in the amount of secreted intermedilysin when cells were grown in a culture medium containing high concentrations of glucose/utilizable carbohydrates. Disruption of the ccpA gene, which encodes catabolite control protein A, did not induce catabolite repression of ily by glucose/utilizable carbohydrates. In cre mutants, catabolite repression of ily was partially restored, and purified catabolite control protein A bound to an oligonucleotide containing the cre consensus sequence in the ily promoter region. In addition, a prolonged lag phase and slower doubling time of the ccpA mutant cells were observed. Our data show that S. intermedius can modulate ily expression and growth rate through catabolite control protein A-mediated monitoring of the extracellular glucose/utilizable carbohydrate concentration. (キーワード) 病原因子 (virulence factor) (出版サイトへのリンク) ● Publication site (DOI): 10.1128/IAI.00113-10 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20624907 ● Search Scopus @ Elsevier (PMID): 20624907 ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.00113-10 (DOI: 10.1128/IAI.00113-10, PubMed: 20624907)
55.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Investigation of the chaperone function of the small heat shock protein AgsA., BMC Biochemistry, Vol.11, 27, 2010. (要約) A small heat shock protein AgsA was originally isolated from Salmonella enterica serovar Typhimurium. We previously demonstrated that AgsA was an effective chaperone that could reduce the amount of heat-aggregated proteins in an Escherichia coli rpoH mutant. AgsA appeared to promote survival at lethal temperatures by cooperating with other chaperones in vivo. To investigate the aggregation prevention mechanisms of AgsA, we constructed N- or C-terminal truncated mutants and compared their properties with wild type AgsA. AgsA showed significant overall homology to wheat sHsp16.9 allowing its three-dimensional structure to be predicted. Truncations of AgsA until the N-terminal 23rd and C-terminal 11th amino acid (AA) from both termini preserved its in vivo chaperone activity. Temperature-controlled gel filtration chromatography showed that purified AgsA could maintain large oligomeric complexes up to 50 degrees C. Destabilization of oligomeric complexes was observed for N-terminal 11- and 17-AA truncated AgsA; C-terminal 11-AA truncated AgsA could not form large oligomeric complexes. AgsA prevented the aggregation of denatured lysozyme, malate dehydrogenase (MDH) and citrate synthase (CS) but did not prevent the aggregation of insulin at 25 degrees C. N-terminal 17-AA truncated AgsA showed no chaperone activity towards MDH. C-terminal 11-AA truncated AgsA showed weak or no chaperone activity towards lysozyme, MDH and CS although it prevented the aggregation of insulin at 25 degrees C. When the same amount of AgsA and C-terminal 11-AA truncated AgsA were mixed (half of respective amount required for efficient chaperone activities), good chaperone activity for all substrates and temperatures was observed. Detectable intermolecular exchanges between AgsA oligomers at 25 degrees C were not observed using fluorescence resonance energy transfer analysis; however, significant exchanges between AgsA oligomers and C-terminal truncated AgsA were observed at 25 degrees C. Our data demonstrate that AgsA has several regions necessary for efficient chaperone activity: region(s) important for lysozyme chaperone activity are located outer surface of the oligomeric complex while those region(s) important for insulin are located inside the oligomeric complex and those for MDH are located within the N-terminal arm. In addition, the equilibrium between the oligomer and the dimer structures appears to be important for its efficient chaperone activity. (キーワード) シャペロン (徳島大学機関リポジトリ) ● Metadata: 114744 (出版サイトへのリンク) ● Publication site (DOI): 10.1186/1471-2091-11-27 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20653971 ● Search Scopus @ Elsevier (PMID): 20653971 ● Search Scopus @ Elsevier (DOI): 10.1186/1471-2091-11-27 (徳島大学機関リポジトリ: 114744, DOI: 10.1186/1471-2091-11-27, PubMed: 20653971)
56.	Fumihiko Namba, Hiroyuki Kitajima, Atsushi Tabata, Masahiro Nakayama, Noriyuki Suehara, Katsura Matsunami, Keiko Yanagihara, Masahiro Nishihara, Akihiro Morita, Minoru Yamada, Akihito Kimoto, Shinya Hirano, Kiyotoshi Sekiguchi, Masanori Fujimura and Itaru Yanagihara : Anti-Annexin A2 IgM Antibody in Preterm Infants: Its Association with Chorioamnionitis, Pediatric Research, Vol.60, No.6, 699-704, 2006. (要約) Intrauterine infection is associated with chorioamnionitis (CAM), which can lead to preterm delivery. We previously reported that the levels of IgM and the incidence of CAM were elevated in preterm infants with neonatal pulmonary emphysema. The pathogen and target of this IgM remain unclear. By using Western blot and amino acid sequences, we have determined one of the target proteins: annexin A2. Immunohistochemical analysis showed that annexin A2 was expressed at fetal chorion and amnion membranes. Among very low birth weight (VLBW) infants with hyper-IgM (> or = 30 mg/dL), 58.8% showed a high titer against annexin A2 (more than x 16), which accounted for about 20%-40% of the total IgM. Anti-annexin A2 IgM antibody inhibited plasmin generation. Furthermore, the median of anti-annexin A2 IgM titer from preterm infants who were delivered with high-grade (grade III) CAM was significantly higher than those from preterm infants without CAM (p = 0.011) and with low-grade CAM (grade I and II) (p = 0.010). Here, we indicate the fetal autoimmunoreactivity against the fetomaternal interface in preterm infants. (キーワード) Amino Acid Sequence / Annexin A2 / Chorioamnionitis / Female / Fibrinolysin / Humans / Immunoglobulin M / Infant, Newborn / Infant, Premature / Infant, Very Low Birth Weight / Molecular Sequence Data / Placenta / Pregnancy / Pregnancy Complications (出版サイトへのリンク) ● Publication site (DOI): 10.1203/01.pdr.0000245910.10929.a6 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17065584 ● Search Scopus @ Elsevier (PMID): 17065584 ● Search Scopus @ Elsevier (DOI): 10.1203/01.pdr.0000245910.10929.a6 (DOI: 10.1203/01.pdr.0000245910.10929.a6, PubMed: 17065584)
57.	Atsushi Tabata, Fumihiko Namba, Minoru Yamada, Taeko Hasegawa, Kumiko Nakahira, Daizo Hamada, Hiroyuki Kitajima, Eiichiro Fukusaki and Itaru Yanagihara : Expression and purification of recombinant human annexin A2 in Pichia pastoris and utility of expression product for detecting annexin A2 antibody, Journal of Bioscience and Bioengineering, Vol.101, No.2, 190-197, 2006. (要約) Annexin A2, a Ca2+-dependent phospholipid binding protein, is abundantly expressed in various human organs, which exists as either a membrane-associated, cytosolic or soluble form in serum. We constructed expression systems for recombinant human annexin A2 (rhA2) using Pichia pastoris. The systems are designed to secrete rhA2 as either the N- or C-terminally His6-tagged form to facilitate purification. Both types of rhA2 were overexpressed, but in the N-terminal-truncated form as revealed from the results of N-terminal amino acid sequencing and Western blotting. Therefore, further purification of N-terminally His6-tagged rhA2 was not feasible because of the removal of the N-terminal His6-tag sequence. C-terminally His6-tagged rhA2 was expressed as either a glycosylated or a nonglycosylated form, and the nonglycosylated form was purified using the combination of nickel-immobilized affinity, concanavalin A and cation exchanged column chromatographies. The solid-phase binding of rhA2 was examined by enzyme-linked immunosorbent assay (ELISA), which revealed the specific reactivity of rhA2 against an anti-annexin A2 monoclonal antibody. These results suggest that the expression system using P. pastoris is useful for the preparation of rhA2 that is applicable to the ELISA detection of the anti-annexin A2 antibody. (キーワード) Amino Acid Sequence / Annexin A2 / Antibodies, Monoclonal / Enzyme-Linked Immunosorbent Assay / Gene Expression Regulation / Histidine / Humans / Molecular Sequence Data / Pichia / Protein Engineering / Recombinant Proteins (出版サイトへのリンク) ● Publication site (DOI): 10.1263/jbb.101.190 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16569618 ● Search Scopus @ Elsevier (PMID): 16569618 ● Search Scopus @ Elsevier (DOI): 10.1263/jbb.101.190 (DOI: 10.1263/jbb.101.190, PubMed: 16569618)
58.	Atsushi Tabata, Hideaki Nagamune, Takuya Maeda, Keiji Murakami, Yoichiro Miyake and Hiroki Kourai : Correlation between resistance of Pseudomonas aeruginosa to quaternary ammonium compounds and expression of outer membrane protein OprR, Antimicrobial Agents and Chemotherapy, Vol.47, No.7, 2093-2099, 2003. (要約) 論文題目:緑膿菌の細胞膜タンパク質OprRの発現と第四アンモニウム塩に対する耐性化の相関第四アンモニウム塩系消毒剤に耐性化した緑膿菌において顕著に発現量が増加する約25.6kDaの蛋白質を2次元電気泳動で分離し，そのN末端アミノ酸配列の解析によって，それが外膜蛋白質のOprRであることを明らかとした．またその耐性化株でOprRのノックアウト株を作成したところ，第四アンモニウム塩系消毒剤に対する感受性が野生株と同程度まで上昇することから，OprRがこの消毒薬耐性に重要な役割を果たしていることを示した． (キーワード) Adaptation, Physiological / Amino Acid Sequence / Bacterial Outer Membrane Proteins / Drug Resistance, Bacterial / Gene Deletion / Genes, Bacterial / Molecular Sequence Data / Mutagenesis / Pseudomonas aeruginosa / Quaternary Ammonium Compounds (徳島大学機関リポジトリ) ● Metadata: 117786 (出版サイトへのリンク) ● Publication site (DOI): 10.1128/AAC.47.7.2093-2099.2003 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12821452 ● Search Scopus @ Elsevier (PMID): 12821452 ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.47.7.2093-2099.2003 (徳島大学機関リポジトリ: 117786, DOI: 10.1128/AAC.47.7.2093-2099.2003, PubMed: 12821452)
59.	Atsushi Tabata, Daxin Zhang, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Microbial Contamination in Home Laundry Operations in Japan, Biocontrol Science, Vol.8, No.1, 9-18, 2003. (要約) 論文題目:日本における家庭洗濯の微生物汚染．家庭洗濯中の衛生レベルは生活様式の変化と共に変遷してきた．諸外国では洗濯中に微生物汚染や感染が報告されている．そこで日本における洗濯中の汚染について様々な着用後の繊維製品に付着している微生物種の同定，洗濯物間の微生物転移による感染の危険性の実態について明らかにした．さらに除菌洗剤と非除菌洗剤を用いて検討し，除菌洗剤の汚染や感染防止効果が高いことを明らかにした． (キーワード) Home laundry / Japan / Microbial contamination / Microbial transmission / Bath water (徳島大学機関リポジトリ) ● Metadata: 117799 (出版サイトへのリンク) ● Publication site (DOI): 10.4265/bio.8.9 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390001204464267648 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.8.9 (徳島大学機関リポジトリ: 117799, DOI: 10.4265/bio.8.9, CiNii: 1390001204464267648)
60.	Atsushi Tabata, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : The Properties of Bacterial Adaptation to Mono-and Bis-Quaternary Ammonium Compounds, Biocontrol Science, Vol.8, No.1, 37-42, 2003. (要約) 論文題目:モノ型およびビス型第四アンモニウム塩に対する細菌の適応の性質．モノ型(デシルピリジニウム塩)およびビス型第四アンモニウム塩(架橋構造がチオール，エステル，カルバモイルおよびアミド)に対する細菌の適応について検討した．最小発育阻止濃度(MIC)測定法により検討した結果，モノ型ではMIC値が上昇し，抵抗性化が見受けられたが，ビス型第四アンモニウム塩では全く耐性化が見られなかった．抵抗性菌の表面疎水性を解析した結果，モノ型の抵抗性化は細胞表面の疎水性が低下したためであるとした． (キーワード) Quaternary ammonium compound / Bis-QAC / Bacterial adaptation / Resistance to disinfectant / Cell surface hydrophobicity (徳島大学機関リポジトリ) ● Metadata: 117798 (出版サイトへのリンク) ● Publication site (DOI): 10.4265/bio.8.37 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390282679441002368 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.8.37 (徳島大学機関リポジトリ: 117798, DOI: 10.4265/bio.8.37, CiNii: 1390282679441002368)
61.	Atsushi Tabata, Takuya Maeda, Hideaki Nagamune and Hiroki Kourai : Characterization of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound, Biocontrol Science, Vol.7, No.3, 147-153, 2002. (要約) 論文題目:第四アンモニウム塩化合物に対して薬剤耐性を示す緑膿菌の特徴 N-ドデシルピリジニウムアイオダイドに対する薬剤耐性化を最小濃度測定法である培地希釈法を用いて行うことに成功した．その結果，感受性菌よりも8倍高い耐性化細菌を得ることができた．電子顕微鏡で観察した結果，耐性化細胞は感受性細胞には見られないバイオフィルム様の凝集が観察された．さらに，耐性化した細胞の表面疎水性について測定した結果，感受性細胞よりも親水性化していることがわかった． (キーワード) Quaternary ammonium compound / Pseudomonas aeruginosa / Disinfectant resistance / Cell surface hydrophobicity / Biofilm (徳島大学機関リポジトリ) ● Metadata: 117800 (出版サイトへのリンク) ● Publication site (DOI): 10.4265/bio.7.147 (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1390001204463971328 ● Search Scopus @ Elsevier (DOI): 10.4265/bio.7.147 (徳島大学機関リポジトリ: 117800, DOI: 10.4265/bio.7.147, CiNii: 1390001204463971328)

学術論文(紀要・その他):

1.	Ogawa Itsuki, Atsushi Tabata, Yukihiro Arakawa, Keiji Minagawa and Fumitoshi Yagishita : Blue Luminescent Boron Complexes Based on N,N-type Bidentate Imidazo[1,2-a]pyridine Ligands, 次世代光フォーラム 2024 in 徳島論文集, Vol.2024, 127-128, 2024.
2.	abe sota, Atsushi Tabata, Yukihiro Arakawa, Keiji Minagawa and Fumitoshi Yagishita : Naphthalene-fused Imidazo[1,2-a]pyridinium Salts Showing Green Emission with High Quantum Yields and Large Stokes Shift, 次世代光フォーラム 2024 in 徳島論文集, Vol.2024, 125-126, 2024.
3.	Fumitoshi Yagishita, Keita Hoshi, Atsushi Tabata, Hideaki Nagamune, Keiji Minagawa and Yasushi Imada : Fluorescent Imidazo[1,2-a]pyridinium Salt for Potent Cancer Therapeutic Agent, Proceedings of the LED General Forum 2021 Tokushima, 155-156, 2021.
4.	Fumitoshi Yagishita, Tanigawa Junichi, Sanagawa Yohei, Okamoto Masaki, Ishihara Kaito, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : Fluorescent N-Heteroarene as a DNA Photo-cleaving Agent under the LED Irradiation, LED総合フォーラム2019 in 徳島論文集, Vol.2019, 71-72, 2019.

国際会議:

1.	Akihiro Shirai, Ami Tanaka and Atsushi Tabata : Influences of light-irradiation wavelength and irradiance on growth and deoxynivalenol production in Fusarium graminearum, ASM Microbe 2024, Vol.4247, No.MBP-FRIDAY-610, Atlanta, Jun. 2024.
2.	Nakai Hiroki, Akiyama Daiu, Taniguchi Yoshiaki, Kishinobu Iori, Ikeda Takuya, Atsushi Tabata, Hideaki Nagamune, Yasuhide Ohno and Masao Nagase : Protein detection by electron donor using epitaxial graphene film on SiC substrate, 33rd International Microprocesses and Nanotechnology Conference (MNC2020), No.2020-21-12, Online, Nov. 2020.
3.	Fumitoshi Yagishita, Sanagawa Yohei, Tanigawa Jun-ichi, Nii Chiho, Atsushi Tabata, Hideaki Nagamune, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Water-Soluble Fluorescent N-Heteroarenes And Its Applications, 18th International Symposium on Novel Aromatic Compounds (ISNA-18), Jul. 2019.
4.	Yamada Takuya, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Response of human cells against peptide type hemolysin Streptolysin S derived from Streptococcus anginosus subsp. anginosus, International Peptide Symposium, Dec. 2018. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / ペプチド (peptide)
5.	Tang Qing, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Characterizatio of anti-HER2 scFv and Fab as a targeting module for novel drug-delivery system against HER2-positive cancer, 24th IUBMB congress & 15th FAOBMB congress, Seoul, Korea, Jun. 2018.
6.	Toshifumi Tomoyasu, Atsushi Tabata, Chiba Shinya, Yamasaki Takahiro, Kusaka Shingo, Takeda Nozomi, Tamaoka Masaaki, Kazuto Ohkura, Whiley A. Robert and Hideaki Nagamune : Positive and negative control mechanisms of pathogenicity expression in Streptococcus intermedius, Lancefield International Symposium on Streptococci and Streptococcal Diseases, Denarau Island Fiji, Oct. 2017. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins) / 免疫 (immunity)
7.	Atsushi Tabata, Deutsch Douglas, Otsuka Seiya, Verratti Kathleen, Toshifumi Tomoyasu, Hideaki Nagamune and Fischetti A. Vincent : Characterization of a novel plasmid discovered in a clinical isolate of Streptococcus anginosus subsp. anginosus, Lancefield International Symposium on Streptococci and Streptococcal Diseases, Denarau Island Fiji, Oct. 2017. (キーワード) 病原微生物 (pathogenic microorganism) / プラスミド
8.	Toshifumi Tomoyasu, Shinya Chiba, Takahiro Yamasaki, Shingo Kusaka, Atsushi Tabata and Hideaki Nagamune : Expression control pathways of ily by blood components in Streptococcus intermedius, The 13th Korea - Japan International Symposium on Microbiology (XIII-KJISM), May 2016. (キーワード) 細胞溶解毒素 (cytolysin) / 糖 (sugar) / 抗体 (antibody) / 連鎖球菌 (Streptococcus)
9.	Hideaki Nagamune, Atsushi Tabata, Yoshitoshi Ogura and Tetsuya Hayashi : Diversity in genome structure showing the genomic dynamism of human-derived Streptococcus mitis, International Symposium on Genome Science 2015: Expanding Frontiers of Genome Science II, Jan. 2015. (キーワード) Streptococcus mitis / ゲノム科学 (genome science)
10.	Hideaki Nagamune, Toshifumi Tomoyasu, Hidenori Imaki, Naoki Yamamoto, Chiharu Taue, 増田早智子, 高尾亞由子, 前田伸子, Atsushi Tabata and Whiley A. Robert : CHARACTERIZATION OF A NOVEL SECRETED GLYCOSIDASE WITH MULTIPLE GLYCOSIDASE ACTIVITIES, MSGA: A CANDIDATE KEY ENZYME REGULATING GROWTH AND PATHOGENICITY OF STREPTOCOCCUS INTERMEDIUS, XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Nov. 2014. (キーワード) 連鎖球菌 (Streptococcus) / 酵素 (enzyme) / 遺伝子発現 (gene expression) / 細胞溶解毒素 (cytolysin)
11.	Atsushi Tabata, Kota Nakano, Yuji Sato, Kentaro Maya, Hiromi Ohtani, Yukimasa Ohkubo, Kazuto Ohkura, Ken Kikuchi, Robert A Whiley, Toshifumi Tomoyasu and Hideaki Nagamune : STREPTOLYSIN S HOMOLOGUES OF PEPTIDE HEMOLYSIN: NOVEL BETA-HEMOLYTIC FACTORS DISTRIBUTED AMONG BETA-HEMOLYTIC ANGINOSUS GROUP STREPTOCOCCI, XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Buenos Aires, Argentina, Nov. 2014. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
12.	Yukihiro Nishikawa, Atsushi Tabata, Toshifumi Tomoyasu, Momoyo Azuma, Yasuhiko Nishioka, Saburo Sone and Hideaki Nagamune : Triple fusion PCR immunochromatography for a rapid and simple diagnosis of penicillin-resistant Streptococcus pneumoniae, IUMS 2011 Sapporo Congress, Sapporo, Japan, Sep. 2011. (キーワード) 連鎖球菌 (Streptococci) / 抗生物質 (antibiotics) / 臨床検査 (clinical laboratory testing)
13.	Kota Nakano, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : Investigation of the novel cytotoxic factor secreted from streptococcus anginosus, IUMS 2011 Sapporo Congress, Sapporo, Sep. 2011.
14.	Hidenori Imaki, Toshifumi Tomoyasu, Sachiko Masuda, Atsushi Tabata and Hideaki Nagamune : Molecular and phenotypic analysis of mutants of ily expressional control factors in Streptococcus intermedius, IUMS 2011 Sapporo Congress, Palermo, Italy, Sep. 2011. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
15.	Atsushi Tabata, Yasuhiro Yamamoto, Ayuko Takao, Toshifumi Tomoyasu, Ayako Nakayama, Nobuko Maeda, Hisashi Ohkuni and Hideaki Nagamune : Investigation of pathogenic properties of cholesterol-dependent cytolysin producing Streptococcus mitis, XVIII Lancefield International Symposium, Palermo, Italy, Sep. 2011. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
16.	Toshifumi Tomoyasu, Sachiko Masuda, Hidenori Imaki, Atsushi Tabata and Hideaki Nagamune : Genetic screening of ily expression control factors in Streptococcus intermedius, XVIII Lancefield International Symposium, Palermo, Italy, Sep. 2011. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
17.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Nuclear Translocation of NF-kB induced by Streptococcus intermedius intermedilysin, International Conference of Indonesian Society for Microbiology: Recent advances of Microbiology in Health, Agriculture, Bioindustry, Surabayai, Nov. 2009.
18.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Functions of DnaK chaperone system in Streptococcus intermedius, Cell Stress Society International, Sapporo, Oct. 2009. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
19.	Susilowati Heni, Hirohiko Okamura, Katsuhiko Hirota, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The molecular mechanism for Streptococcus intermedius intermedilysin-induced cell death inhuman cholangiocellular carcinoma cells, International Joint Symposium Frontier in Biomedical Sciences: From Genes to Applications, Yogyakarta, Nov. 2008.
20.	Atsushi Tabata, Shizuka Saito, Eriko Sakakura, Yuki Shinohara, Soichiro Miyoshi, Kou Fukushima, Toshifumi Tomoyasu, Hisashi Ohkuni and Hideaki Nagamune : Streptococcus mitis-derived human platelet aggregation factor is a novel type of cholesterol-dependent cytolysin that recognizes dual receptors, cholesterol and human CD59., XVII Lancefield International Symposium on Streptococci & Streptococcal Diseases, Porto Heli, Greece, Jun. 2008.
21.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : Intermedilysin induces cell death in HepG2 cells, 21st International Association for Dental Research - South East Asia Division, Bali, Indonesia, Sep. 2007.
22.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Function of DnaK in Streptococcus intermedius, Cell Stress Society International, Budapest, Aug. 2007. (キーワード) 連鎖球菌 / シャペロン / DnaK
23.	Heni Susilowati, Katsuhiko Hirota, Hirohiko Okamura, Kaya Yoshida, Keiji Murakami, Atsushi Tabata, Hideaki Nagamune, Tatsuji Haneji and Yoichiro Miyake : The mechanisms of Intermedilysin to induce cell death on HepG2 and HuCCT1 cell lines, The 1st international symposium and workshop ''The Future Direction of Oral Sciences in the 21st century'', Awajishima, Mar. 2007.
24.	Atsushi Tabata, Akiko Inubushi, Toshifumi Tomoyasu and Hideaki Nagamune : Immunochemical analysis of a human specific cytolysin, intermedilysin, for elucidation of its mode of action to human cells, 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto, Jun. 2006.
25.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Small heat shock protein ``AgsA'' suppresses aggregation of heat denatured proteins., 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto, Jun. 2006.

国内講演発表:

1.	高嶋宙, 松村大夢, 大野恭秀, 永瀬雅夫, 田端厚之, 長宗秀明 : 抗体配向修飾SiC上グラフェン膜を用いた蛍光・電気測定による標的検出, 2024年度応用物理・物理系学会中国四国支部合同学術講演会, Vol.Fp-7, 2024年7月.
2.	松村大夢, 森優介, 髙嶋宙, 大野恭秀, 永瀬雅夫, Hoang Anh Tung, 田端厚之, 長宗秀明 : HisタグB-domainを用いたSiC上グラフェンへの抗体配向修飾法, 2024年第71回応用物理学会春季学術講演会, Vol.24a-1BM-7, 2024年3月.
3.	阿部壮太, 上田昭子, 田端厚之, 南川慶二, 今田泰嗣, 八木下史敏 : ナフタレン縮環イミダゾ[1,2-a]ピリジニウム塩の合成と光物性評価, 日本化学会第104春季年会, 2024年3月.
4.	朴素, 梅林隆太, 田端厚之, 荒川幸弘, 南川慶二, 今田泰嗣, 八木下史敏 : サブフタロシアニン誘導体の合成と光細胞傷害性評価, 2023年日本化学会中国四国支部大会山口大会, 2023年11月.
5.	山森優護, 長宗秀明, 友安俊文, 田端厚之 : Streptococcus anginosusが産生するStreptolysin Sに対する宿主細胞応答のメカニズム, 第96回日本生化学会大会, 2023年11月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin)
6.	青山里菜, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius が保有する新規細胞壁アンカータンパク質の機能解析, 第76回日本細菌学会支部総会, 2023年10月. (キーワード) レンサ球菌 / 細胞外マトリックス (extracellular matrix)
7.	田端厚之, 横畑修人, 大倉一人, 友安俊文, 長宗秀明 : レンサ球菌が産生するペプチド溶血毒素ストレプトリジンSの血清アルブミンによる活性保護, 第69回トキシンシンポジウム, 2023年9月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin)
8.	山森優護, 長宗秀明, 友安俊文, 田端厚之 : Streptococcus anginosus が産生する Streptolysin S に対する宿主細胞応答のメカニズム, 第69回トキシンシンポジウム, 2023年9月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / 宿主細胞応答
9.	松本愛理, 大貝悠一, 田端厚之, 住友倫子, 中田匡宣 : ミティス群レンサ球菌における細胞外小胞の産生とその作用特性, 第75回九州微生物研究フォーラム2023, 2023年9月.
10.	田中彩水, 田端厚之, 白井昭博 : Fusarium graminearumの生育とデオキシニバレノール生合成に及ぼす光波長と照度の影響, 日本防菌防黴学会第50回年次大会要旨集, No.2P-Dp05, 200, 2023年8月.
11.	植野美彦, 関陽介, 服部武文, 田端厚之, 向井理恵, 岡直宏, 宇都義浩 : B学部における学校推薦型選抜Ⅰ地方創生型(地域産業振興枠)の設計と実施 ―入試業務効率化に向けた新たな取り組みを踏まえて―, 令和5年度全国大学入学者選抜研究連絡協議会大会(第18回), 2023年5月.
12.	大岡桂一朗, 田端厚之, 大国寿士, 友安俊文, 長宗秀明 : S. mitis由来ヒト血小板凝集因子のN末追加ドメインに依存的な宿主細胞の遺伝子発現亢進, 第96回日本細菌学会総会, 2023年3月. (キーワード) レンサ球菌 / ヒト血小板凝集因子 / 細胞溶解毒素 (cytolysin) / 細胞応答
13.	横畑修人, 大倉一人, 長宗秀明, 友安俊文, 田端厚之 : ヒト血清アルブミンによるStreptolysin Sの細胞傷害活性の安定化, 第96回日本細菌学会総会, 2023年3月. (キーワード) ヒト血清アルブミン / 細胞傷害 / 細胞溶解毒素 (cytolysin)
14.	岡本涼太, 友安俊文, 田端厚之, 長宗秀明 : Brevibacillus brevis DnaKシャペロンシステムの機能解析, 第96回日本細菌学会総会, 2023年3月. (キーワード) Brevibacillus / 分子シャペロン (molecular chaperone) / DnaK / グラム陽性菌
15.	田端厚之, 松本愛理, 藤本あい, 友安俊文, 髙尾亜由子, 大国寿士, 長宗秀明 : S. mitis由来新規5ドメイン型コレステロール依存性細胞溶解毒素Discoidinolysinの分子特性, 第96回日本細菌学会総会, 2023年3月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / 分子特性
16.	山森優護, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus anginosusが産生するStreptolysin Sに対する細胞応答とそのメカニズム, 第96回日本細菌学会総会, 2023年3月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / 細胞応答
17.	竹下凌哉, 田端厚之, 友安俊文, 長宗秀明 : 肺がん細胞指向性DDS ツールの作製に向けたリガンド分子の構築, 第146回徳島生物学会, 2023年1月. (キーワード) 肺がん / DDS ツール / 癌治療 (cancer therapy)
18.	森優介, 松村大夢, 村山圭汰, 竹下凌哉, HOANG ANH TUNG, 大野恭秀, 永瀬雅夫, 田端厚之, 長宗秀明 : His-tag 法を用いた SiC 上グラフェンへの抗体配向修飾技術, 第39回「センサ・マイクロマシンと応用システム」シンポジウム, Vol.16P2-P-52, 2022年11月.
19.	武澤晃司, 田端厚之, 長宗秀明, 友安俊文 : ミセルを用いた癌治療用新規DDSツールの開発とその評価, 第95回日本生化学会大会, 2022年11月. (キーワード) ミセル / DDS / 癌治療 (cancer therapy)
20.	田端厚之, 松本愛理, 藤本あい, 池田拓也, 友安俊文, 髙尾亜由子, 大国寿士, 長宗秀明 : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の分子特性と病原性への寄与, 第52回レンサ球菌研究会, 2022年7月. (キーワード) レンサ球菌 / コレステロール依存性細胞溶解毒素 / 病原性
21.	清水桐也, 友安俊文, 多田雄哉, 田端厚之, 長宗秀明 : Streptococcus intermedius が保有する細胞壁アンカー型エンド-β-N-アセチルグルコサミニダーゼの機能解析, 第52回レンサ球菌研究会, 2022年7月. (キーワード) レンサ球菌 / 糖鎖 / エンド-β-N-アセチルグルコサミニダーゼ
22.	田端厚之, 横畑修人, 友安俊文, 長宗秀明 : レンサ球菌が産生するペプチド溶血毒素ストレプトリジンSに対するTHP-1の応答反応, 第63回日本生化学会中国四国支部例会, 2022年5月. (キーワード) レンサ球菌 / 溶血毒素 / 病原性
23.	田端厚之 : ヒト口腔常在性日和見レンサ球菌が産生する溶血毒素の構造的および機能的多様性と病原性への寄与, 第95回日本細菌学会総会, 2022年3月.
24.	友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusの新規細胞壁アンカー型グリコシダーゼの機能解析, 第95回細菌学会総会, 2022年3月. (キーワード) 連鎖球菌 (Streptococci) / グリコシダーゼ / 糖タンパク質 / 糖鎖
25.	尾田優紀, 松本愛理, 田端厚之, 友安俊文, 大国寿士, 長宗秀明 : Streptococcus mitis 由来Mitilectinホモログの産生特性及び分子特性の解析, 第95回細菌学会総会, 2022年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 宿主細胞定着因子
26.	横畑修人, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus phocae由来β溶血因子の産生特性および作用特性, 第95回細菌学会総会, 2022年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / ストレプトリジンS / 細胞障害性
27.	久保百果, 田端厚之, 池田拓也, 友安俊文, 大国寿士, 長宗秀明 : 川崎病患児由来S. mitis株の膜孔形成毒素に対するヒトマクロファージ様細胞の応答反応, 第95回細菌学会総会, 2022年3月. (キーワード) 連鎖球菌 (Streptococci) / 川崎病 / 細胞溶解毒素 (cytolysin) / 炎症性サイトカイン (inflammatory cytokine)
28.	横畑修人, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus phocae が産生するβ溶血因子の作用特性と病原性への寄与, 第74回⽇本細菌学会中国・四国⽀部総会, 2021年10月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / ストレプトリジンS / コレステロール依存性細胞溶解毒素
29.	田原晃生, 板家將海, 田端厚之, 長宗秀明, 片山哲郎, 古部昭広, 南川慶二, 今田泰嗣, 八木下史敏 : イミダゾ[1,5-a]ピリジン三座配位子-ホウ素錯体の合成および構造と光物性評価, 2021年光化学討論会, 2021年9月.
30.	友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusが保有するType VII輸送装置の遺伝学的解析, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / Type VII輸送装置 / 病原性
31.	三木晴加, 田端厚之, 菊池賢, 劉瀚宇, 友安俊文, 長宗秀明 : Gemella bergeri 由来コレステロール依存性細胞溶解毒素の細胞障害性に関する検討, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / 細胞障害性
32.	田端厚之, 大国寿士, 友安俊文, 長宗秀明 : Streptococcus mitis Nm-65株の潜在的病原性, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 病原因子 (virulence factor) / ゲノム情報
33.	一條安由, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusの細胞壁アンカータンパク質群の精製, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 細胞外マトリックス (extracellular matrix) / 細胞壁アンカータンパク質
34.	池田拓也, 田端厚之, 友安俊文, 高尾亜由子, 大国寿士, 長宗秀明 : N末追加ドメインを持つS.mitis由来5ドメイン型コレステロール依存性細胞溶解毒素の2元的な宿主細胞結合性, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / N末追加ドメイン
35.	白井里奈, 田端厚之, 友安俊文, 長宗秀明 : S. anginosusが産生するストレプトリジンSに対するTHP-1の応答反応の検討, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 細胞溶解毒素 (cytolysin) / 免疫担当細胞
36.	松本愛理, 田端厚之, 大倉一人, 児玉千紘, 大国寿士, 高尾亞由子, 菊池賢, 友安俊文, 長宗秀明 : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素モチーフを持つ細胞接着分子の特性, 第94回日本細菌学会総会, 2021年3月. (キーワード) レンサ球菌 / 細胞接着分子
37.	八木下史敏, 國見祥太, 星恵太, 岡本将輝, 田端厚之, 長宗秀明, 長谷栄治, 南川慶二, 今田泰嗣 : 多光子励起可能なイミダゾ[1,2-a]ピリジニウム塩の合成と光細胞傷害性, 日本化学会第101春季年会, 2021年3月.
38.	楠本一真, 髙成広起, 田端厚之, 長宗秀明, 荒川幸弘, 南川慶二, 今田泰嗣, 八木下史敏 : D-π-A型ベンゾチアゾール誘導体の合成と光機能性, 2020年日本化学会中国四国支部大会島根大会, 2020年11月.
39.	松本愛理, 田端厚之, 高尾亜由子, 菊池賢, 友安俊文, 長宗秀明 : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素様の細胞接着分子の特性, 第73回日本細菌学会中国・四国支部総会, 2020年10月.
40.	楠本一真, 佐名川洋平, 長谷栄治, 髙成広起, 田端厚之, 長宗秀明, 荒川幸弘, 南川慶二, 今田泰嗣, 八木下史敏 : 多光子励起を目指したA-D-A型N-へテロアレーン類のデザインと応用, 2020年web光化学討論会, 2020年9月.
41.	梅林隆太, 岡本将輝, 長谷栄治, 髙成広起, 田端厚之, 長宗秀明, 荒川幸弘, 南川慶二, 今田泰嗣, 八木下史敏 : 多光子励起可能な水溶性イミダゾ[1,2-a]ピリジニウム塩の合成とミトコンドリアイメージング, 2020年web光化学討論会, 2020年9月.
42.	松本愛理, 田端厚之, 大倉一人, 児玉千紘, 大国寿士, 高尾亞由子, 菊池賢, 友安俊文, 長宗秀明 : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素モチーフを持つ細胞接着分子の特性, 第15回細菌学若手コロッセウム, 2020年8月. (キーワード) レンサ球菌 / 細胞接着分子
43.	田端厚之, 大倉一人, 大国寿士, 小林未歩, 松本愛理, 友安俊文, 長宗秀明 : The structural and functional diversity of cholesterol-dependent cytolysin produced from Streptococci, 第20回日本蛋白質科学会年会, 2020年7月.
44.	松本愛理, 田端厚之, 高尾亞由子, 菊池賢, 友安俊文, 長宗秀明 : Cholesterol-dependent cytolysin-like adhesion protein of Mitis group streptococci, 第20回日本蛋白質科学会年会, 2020年7月.
45.	Fumitoshi Yagishita, Junichi Tanigawa, Masaki Okamoto, Eiji Hase, Hiroki Takanari, Atsushi Tabata, Hideaki Nagamune, Takeo Minamikawa, Takeshi Yasui, Yasushi Imada and Yasuhiko Kawamura : Synthesis of Imidazopyridinium Salts Having Potential Application as Image-guided Photodynamic Therapy Agents, The 100th CSJ Annual Meeting, Mar. 2020.
46.	田端厚之 : アンギノーサス群レンサ球菌が産生するペプチド溶血毒素Streptolysin Sの特徴と細胞障害性, 第93回日本細菌学会総会, 2020年2月.
47.	友安俊文, 出口真理, 田端厚之, 髙尾亞由子, 前田伸子, 長宗秀明 : Streptococcus intermediusのヒアルロニダーゼ発現制御機構についての解析, 第93回日本細菌学会総会, 2020年2月. (キーワード) ヒアルロニダーゼ / 遺伝子発現 (gene expression) / 連鎖球菌 (Streptococci)
48.	松本愛理, 田端厚之, 髙尾亞由子, 菊池賢, 友安俊文, 前田伸子, 長宗秀明 : ミチス群レンサ球菌が産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第93回日本細菌学会総会, 2020年2月. (キーワード) 細胞接着因子 / 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
49.	児玉千紘, 田端厚之, 友安俊文, 髙尾亞由子, 前田伸子, 長宗秀明 : S. infantisが産生するコレステロール依存性細胞溶解毒素infantilysinの特性解析, 第93回日本細菌学会総会, 2020年2月. (キーワード) 細胞溶解毒素 (cytolysin) / コレステロール (cholesterol) / 連鎖球菌 (Streptococci)
50.	八木下史敏, 岡本将輝, 髙成広起, 長谷栄治, 田端厚之, 長宗秀明, 今田泰嗣, 安井武史, 河村保彦 : ミトコンドリアイメージングを可能とするイミダゾ[1,2-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, 2019年11月.
51.	八木下史敏, 谷川純一, 新居千穂, 田端厚之, 長宗秀明, 髙成広起, 今田泰嗣, 河村保彦 : 光線力学療法への応用を狙いとしたイミダゾ[1,5-a]ピリジニウム塩の合成, 2019年日本化学会中国四国支部大会徳島大会, 2019年11月.
52.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : S. pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第92回日本生化学会大会, 2019年9月. (キーワード) 細胞接着 / レンサ球菌 / 病原因子 (virulence factor)
53.	友安俊文, 冨永明子, 出口真理, 有本江里, 田端厚之, 高尾亞由子, 前田伸子, 長宗秀明 : Streptococcus intermedius が分泌する病原因子の特性解析, 第66回トキシンシンポジウム, 2019年9月. (キーワード) 細胞溶解毒素 (cytolysin) / ヒアルロニダーゼ / レンサ球菌
54.	八木下史敏, 梅林隆太, 岡本将輝, 田端厚之, 長宗秀明, 髙成広起, 今田泰嗣, 河村保彦 : D-π-A 型イミダゾ [1,2-a] ピリジニウム塩の合成とミトコンドリアイメージングへの応用, 2019年光化学討論会, 2019年9月.
55.	立松三千子, 萩原純孝, 田端厚之, 服部正也, 小谷はるる, 水野靖也, 澤木正孝, 石黒淳子, 吉村章代, 片岡愛弓, 肱岡範, 清水淳市, 岩田広治 : エベロリムス服用患者におけるステロイド含有含嗽薬使用時の口腔内微生物叢の変動についての検討, 第4回日本がんサポーティブケア学会学術集会, 2019年9月.
56.	児玉千紘, 唐卿, 田端厚之, 髙尾亞由子, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantisが産生する新規コレステロール依存性細胞溶解毒素infantilysinの作用特性の解明, 第13回細菌学若手コロッセウム, 2019年8月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
57.	一條安由, 友安俊文, 有本江里, 出口真理, 田端厚之, 高尾亞由子, 前田伸子, 長宗秀明 : Streptococcus intermediusのヒアルロニダーゼ発現制御機構の解析, 第13回細菌学若手コロッセウム, 2019年8月. (キーワード) 連鎖球菌 / ヒアルロン酸分解酵素 / 細胞外マトリックス (extracellular matrix)
58.	白井里奈, 小林未歩, 田端厚之, 大国寿士, 友安俊文, 長宗秀明 : Streptococcus mitisが産生する非典型CDCに対する細胞の応答反応, 第13回細菌学若手コロッセウム, 2019年8月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
59.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第13回細菌学若手コロッセウム, 2019年8月. (キーワード) 連鎖球菌 (Streptococci) / 細胞接着分子 / 細胞溶解毒素 (cytolysin)
60.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様の細胞接着分子, 第51回レンサ球菌研究会, 2019年6月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 細胞接着分子
61.	田端厚之, 友安俊文, 長宗秀明 : Streptococcus anginosus subsp. anginosus由来溶原性ファージの遺伝子的特性, 第51回レンサ球菌研究会, 2019年6月. (キーワード) 連鎖球菌 (Streptococcus) / バクテリオファージ (bacteriophage)
62.	友安俊文, 出口真理, 高尾亞由子, 田端厚之, 前田伸子, 長宗秀明 : Streptococcus intermediusが保有する分泌型グリコシダーゼの局在性, 第92回日本細菌学会総会, 2019年4月. (キーワード) 連鎖球菌 (Streptococcus) / グリコシダーゼ / 局在性
63.	田端厚之, 友安俊文, 長宗秀明 : Streptococcus anginosus由来溶原性ファージの特性, 第92回日本細菌学会総会, 2019年4月. (キーワード) 連鎖球菌 (Streptococcus) / バクテリオファージ (bacteriophage)
64.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : S. pseudopneumoniaeが産生するコレステロール依存性細胞溶解毒素様分子の特性解析, 第92回日本細菌学会総会, 2019年4月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 進化 (evolution)
65.	児玉千紘, 唐卿, 田端厚之, 高尾亞由子, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantisが産生するinfantilysinの分子特性, 第92回日本細菌学会総会, 2019年4月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
66.	小林未歩, 田端厚之, 大国寿士, 高尾亞由子, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitisが産生する5ドメイン型CDCに対するヒト好中球様細胞の応答反応, 第92回日本細菌学会総会, 2019年4月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
67.	八木下史敏, 谷川純一, 新居千穂, 田端厚之, 長宗秀明, 今田泰嗣, 河村保彦 : 水溶性N-へテロアレーンを用いたHeLa細胞の蛍光イメージングと光細胞毒性, 日本化学会第99春季年会, 2019年3月.
68.	田端厚之, 宇都義浩, 堀均, 大倉一人 : Chiral-2-nitroimidazole骨格を有するTX-2036 誘導体の開発:放射線増感能の修飾に関わる分子特性の検証, 第22回バイオ治療法研究会学術集会, 2018年12月.
69.	西平有里菜, 田端厚之, 友安俊文, 大倉一人, 長宗秀明 : 薬剤キャリアのミセルが示す癌細胞障害性, 第22回バイオ治療法研究会学術集会, 2018年12月. (キーワード) ドラッグデリバリーシステム (drug delivery system) / 癌治療 (cancer therapy)
70.	八木下史敏, 谷川純一, 新居千穂, 田端厚之, 長宗秀明, 今田泰嗣, 河村保彦 : 光照射をトリガーとした水溶性N-ヘテロアレーンによるDNA損傷, 2018年日本化学会中国四国支部大会, 2018年11月.
71.	出口真理, 友安俊文, 高尾亞由子, 田端厚之, 長宗秀明 : Streptococcus intermediusが保有するグリコシダーゼの局在性についての解析, 第71回日本細菌学会中国・四国支部総会, 2018年10月. (キーワード) 連鎖球菌 (Streptococcus) / グリコシダーゼ / 局在性
72.	山田拓矢, 田端厚之, 友安俊文, 長宗秀明 : S.anginosus由来Streptolysin Sホモログに対する宿主ヒト培養細胞の応答反応, 第91回日本生化学会大会, 2018年9月.
73.	谷川純一, 八木下史敏, 新居千穂, 田端厚之, 長宗秀明, 今田泰嗣, 河村保彦 : 水溶性N-ヘテロアレーンの蛍光バイオイメージングへの応用とDNA損傷, 2018年光化学討論会, 2018年9月.
74.	谷川純一, 八木下史敏, 新居千穂, 田端厚之, 長宗秀明, 今田泰嗣, 河村保彦 : 蛍光発光性N-ヘテロアレーンのバイオイメージングへの応用とDNA損傷, 第48回複素環化学討論会, 2018年9月.
75.	八木下史敏, 畦﨑翔太, 手塚美彦, 田端厚之, 長宗秀明, 河村保彦 : スチリル基を有するイミダゾ[1,5-a]ピリジン二量体の金属イオン存在下における発光挙動, 第48回複素環化学討論会, 2018年9月.
76.	佐名川洋平, 八木下史敏, 田端厚之, 長宗秀明, 河村保彦 : A-π-D-π-A システムに基づく新規対称発光分子のデザインと合成，及び光物性, 第48回複素環化学討論会, 2018年9月.
77.	児玉千紘, 唐卿, 田端厚之, 髙尾亞由子, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantisが産生するInfantilysinの作用特性を明らかにする, 第12回細菌学若手コロッセウム in OKAYAMA, 2018年8月.
78.	藤本あい, 田端厚之, 髙尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第12回細菌学若手コロッセウム in OKAYAMA, 2018年8月.
79.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus pseudopneumoniaeが保有するコレステロール依存性細胞溶解毒素様分子の解析, 第12回細菌学若手コロッセウム in OKAYAMA, 2018年8月.
80.	田端厚之, 松本愛理, 藤本あい, 児玉千紘, 友安俊文, 長宗秀明 : 咽頭口腔レンサ球菌が産生する溶血毒素とその多様性, 第12回細菌学若手コロッセウム in OKAYAMA, 2018年8月.
81.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : S. pseudopneumoniaeが保有するコレステロール依存性細胞溶解毒素様分子の解析, 第65回トキシンシンポジウム, 2018年7月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
82.	藤本あい, 田端厚之, 髙尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性, 第65回トキシンシンポジウム, 2018年7月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / コレステロール (cholesterol)
83.	冨永明子, 友安俊文, 日下竜馬, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 長宗秀明 : グループ3に属するコレステロール依存性細胞溶解毒素のCD59認識性の比較, 第65回トキシンシンポジウム, 2018年7月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / コレステロール (cholesterol)
84.	田端厚之, 大塚誠也, Deutsch Douglas, 菊池賢, Fischetti A. Vincent, 友安俊文, 長宗秀明 : Streptococcus anginosus subsp. anginosusが保有するプラスミドとその特徴, 第50回レンサ球菌研究会, 2018年6月. (キーワード) 連鎖球菌 (Streptococcus) / プラスミド
85.	友安俊文, 的場正樹, 日下信吾, 田端厚之, 長宗秀明 : Streptococcus intermediusのグリコシダーゼとインターメディリシン産生との関連について, 第50回レンサ球菌研究会, 2018年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原因子 (virulence factor) / 細胞溶解毒素 (cytolysin) / 糖 (sugar)
86.	立松洋平, 田端厚之, 長宗秀明, 大倉一人 : 非ステロイド性抗炎症薬セレコキシブの細胞膜障害メカニズムの解析, 第91回日本細菌学会総会, 2018年3月.
87.	日野はるか, 岡畑達也, 田端厚之, 髙尾亞由子, 大國寿士, 小椋義俊, 友安俊文, 林哲也, 前田伸子, 長宗秀明 : Streptococcus mitisにおけるコレステロール依存性細胞溶解毒素の遺伝子分布パターンと細胞障害性との関連性, 第91回日本細菌学会総会, 2018年3月.
88.	荒木理紗子, 田端厚之, 村上漱, 髙尾亞由子, 大国寿士, 小椋義俊, 友安俊文, 林哲也, 前田伸子, 長宗秀明 : タンデム型末追加ドメインを持つコレステロール依存性細胞溶解毒素の特性解析, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
89.	山田拓矢, 田端厚之, 友安俊文, 長宗秀明 : Transcriptional response of host cells induced by SLS-producing S. anginosus subsp. anginosus, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
90.	高尾亞由子, 友安俊文, 田端厚之, 長宗秀明, 前田伸子 : グリコシダーゼ活性が Streptococcus intermedius の実験的バイオフィルム量に及ぼす影響, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
91.	岩浅弥生, 福富あすか, 田端厚之, 村上漱, 友安俊文, 長宗秀明 : Multiplex-PCR による高病原性 S. mitis 検出系の実用化に向けた検討, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
92.	田端厚之, 大塚誠也, 菊池賢, 友安俊文, 長宗秀明 : S. anginosus subsp. anginosusにおけるプラスミドの保有状況とそれらの特性, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
93.	友安俊文, 冨永明子, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 長宗秀明 : DTT処理赤血球を用いたコレステロール依存性細胞溶解毒素の CD59認識性の比較, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
94.	藤本あい, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : S. mitis由来の新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第91回日本細菌学会総会, 2018年3月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
95.	谷脇竜弥, 杉田知世, 村上圭史, 天羽崇, 田端厚之, 山村佳子, 玉谷哲也, 長宗秀明, 宮本洋二, 藤猪英樹 : 幼児口腔内創部より検出された多剤耐性レンサ球菌について, 四国歯学会第52回例会, 2018年3月.
96.	八木下史敏, 新居千穂, 手塚美彦, 田端厚之, 長宗秀明, 上田昭子, 河村保彦 : Synthesis and Evaluation of Photophysical Properties of Dimeric Imidazo[1,5-a]pyridinium Salts, 日本化学会第98春季年会, 2018年3月.
97.	冨永明子, 友安俊文, 日下竜馬, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 長宗秀明 : グループ3に属するコレステロール依存性細胞溶解毒素のCD59認識性の比較, 2017年度生命科学系学会合同年次大会, 2017年12月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
98.	日下信吾, 田端厚之, 友安俊文, 長宗秀明 : シアリダーゼ阻害剤がStreptococcus intermediusのシアリダーゼ活性や溶血活性に及ぼす効果, 2017年度生命科学系学会合同年次大会, 2017年12月. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
99.	八田和己, 田端厚之, 友安俊文, 長宗秀明 : ナノ粒子表面加工システムの構築に用いる細菌由来ペプチド転移酵素Sortase A組換え体の作製とその特性, 2017年度生命科学系学会合同年次大会, 2017年12月. (キーワード) 癌治療 (cancer therapy)
100.	岡田千穂, 田端厚之, 友安俊文, 長宗秀明 : DDS用エフェクター分子としての細菌毒素組換え体の効率的な調製システムの構築, 2017年度生命科学系学会合同年次大会, 2017年12月. (キーワード) 癌治療 (cancer therapy)
101.	立松洋平, 川口遊喜, 田端厚之, 宇都義浩, 堀均, 大倉一人 : 糖付加によるTX-1877系列化合物の機能制御:放射線増感能との相関解析, 第21回バイオ治療法研究会, 2017年12月.
102.	唐卿, 鬼塚正義, 田端厚之, 友安俊文, 長宗秀明 : 癌治療用新規DDSツール作製に用いるリガンド分子の調製とその機能評価, 第21回バイオ治療法研究会, 2017年12月. (キーワード) 癌治療 (cancer therapy)
103.	藤本あい, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : Streptococcus mitis由来新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性を規定する構造領域の探索, 第70回日本細菌学会中国・四国支部総会, 2017年10月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
104.	友安俊文, 日下信吾, 千葉真也, 山崎貴大, 田端厚之, 長宗秀明 : ヒト血漿によるStreptococcus intermediusの病原性発現抑制機構の解析, 第70回日本細菌学会中国・四国支部総会, 2017年10月. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
105.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : S. mitis由来5ドメイン型コレステロール依存性細胞溶解毒素のN末追加ドメインが示す細胞障害作用, 第11回細菌学若手コロッセウム, 2017年8月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
106.	藤本あい, 田端厚之, 髙尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : Streptococcus mitis が産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性に関する研究, 第11回細菌学若手コロッセウム, 2017年8月. (キーワード) 病原微生物 (pathogenic microorganism) / 細菌毒素 (bacterial toxins)
107.	友安俊文, 田端厚之, 千葉真也, 山崎貴大, 竹田望, 玉岡雅章, 大倉一人, 長宗秀明 : 糖によるStreptococcus intermediusの病原性制御機構, 第64回トキシンシンポジウム, 2017年7月. (キーワード) 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
108.	岡畑達也, 田端厚之, 小椋義俊, 林哲也, 高尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitis におけるコレステロール依存性細胞溶解毒素遺伝子の周辺遺伝子配座の多様性とその特徴, 第58回日本生化学会中国・四国支部例会, 2017年5月. (キーワード) 細菌毒素 (bacterial toxins) / 遺伝子 (gene)
109.	大塚誠也, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus anginosus subsp. anginosusが保有する新規Extra-chromosomal DNAの分子特性, 第90回日本細菌学会総会, 2017年3月. (キーワード) 連鎖球菌 (Streptococcus) / プラスミド / 可動遺伝子
110.	的場正樹, 友安俊文, 高尾亞由子, 田端厚之, 前田伸子, 長宗秀明 : Streptococcus intermediusの高病原性株スクリーニングシステムの開発, 第90回日本細菌学会総会, 2017年3月. (キーワード) 連鎖球菌 (Streptococcus) / 病原因子 (virulence factor) / 細胞溶解毒素 (cytolysin)
111.	岡畑達也, 村上漱, 田端厚之, 小椋義俊, 林哲也, 高尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitisが保有するコレステロール依存性細胞溶解毒素遺伝子周辺における遺伝子配座の多様性, 第90回日本細菌学会総会, 2017年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / ゲノム (genome)
112.	松本愛理, 田端厚之, 友安俊文, 長宗秀明 : S. mitis由来5ドメイン型コレステロール依存性細胞溶解毒素のN末追加ドメインが示す細胞障害作用, 第90回日本細菌学会総会, 2017年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
113.	日下竜馬, 友安俊文, 田端厚之, 長宗秀明 : ウマ赤血球に存在するExD-CDCレセプターの探索, 第3回日本生物工学会西日本支部講演会, 2016年12月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / CD59
114.	藤本あい, 田端厚之, 高尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : Streptococcus mitisが産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性の多様性, 第3回日本生物工学会西日本支部講演会, 2016年12月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
115.	西郷琢也, 田端厚之, 高尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : Streptococcus mitisにおける効果的な形質転換法の検討, 第3回日本生物工学会西日本支部講演会, 2016年12月. (キーワード) 連鎖球菌 (Streptococcus) / 形質転換法 / プラスミド / DNA断片
116.	田端厚之, 大倉一人, 立松洋平, 友安俊文, 長宗秀明 : ヒト特異的な細胞障害性を示す細菌毒素に対する糖の阻害作用, 第20回バイオ治療法研究会, 2016年12月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 糖 (sugar)
117.	日下信吾, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius が産生するシアリダーゼの発現制御機構の解析, 第69回日本細菌学会中国・四国支部総会, 2016年10月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 糖 (sugar)
118.	田村郁実, 田端厚之, 村上漱, 髙尾亞由子, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantis が産生する新規コレステロール依存性細胞溶解毒素Infantilysin, 第63回トキシンシンポジウム, 2016年7月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin)
119.	友安俊文, 千葉真也, 山崎貴大, 日下慎吾, 田端厚之, 長宗秀明 : Streptococcus intermedius の血液成分による病原性亢進とヒト血液成分によるその防御, 第48回レンサ球菌研究会, 2016年7月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 糖 (sugar) / 抗体 (antibody)
120.	田村郁実, 田端厚之, 村上漱, 高尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantis 由来新規コレステロール依存性細胞溶解毒素の細胞障害特性, 第89回日本細菌学会総会, 2016年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 機能 (function)
121.	大谷浩美, 田端厚之, 友安俊文, 長宗秀明 : β 溶血性S. anginosus subsp. anginosus のSLS ホモログ依存的な細胞障害性, 第89回日本細菌学会総会, 2016年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 機能 (function) / 遺伝子発現 (gene expression)
122.	友安俊文, 日下慎也, 的場正樹, 金子幸広, 田端厚之, 長宗秀明 : Streptococcus intermedius のily 遺伝子の広い上流領域がその発現抑制に関与する, 第89回日本細菌学会総会, 2016年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
123.	千葉真也, 友安俊文, 山崎貴大, 日下信吾, 田端厚之, 長宗秀明 : 血液成分によるily 遺伝子発現の正および負の調節, 第89回日本細菌学会総会, 2016年3月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 血液 (blood) / 糖 (sugar)
124.	Qing Tang, Atsushi Tabata, Toshifumi Tomoyasu and Hideaki Nagamune : A trial for development of effective drug delivery system using small targeting module against CEA-positive cancer, Joint Meeting of the 38th Annual Meeting of the Molecular Biology Society of Japan and the 88th Annual Meeting of the Japanese Biochemical Society, Dec. 2015. (キーワード) ドラッグデリバリーシステム (drug delivery system) / 抗体 (antibody)
125.	棚次亮介, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius DnaKを用いたグラム陰性菌DnaKに特異的な保存領域の機能解析, 第38回日本分子生物学会年会, 2015年12月. (キーワード) 連鎖球菌 (Streptococcus) / 分子シャペロン (molecular chaperone) / タンパク質 (protein)
126.	日下慎也, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius のily遺伝子発現制御領域の探索, 第38回日本分子生物学会年会, 2015年12月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
127.	千葉真也, 友安俊文, 山崎貴大, 田端厚之, 長宗秀明 : 血液成分によるインターメディリシン発現調節機構の解析, 第68回日本細菌学会中国・四国支部総会, 2015年10月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 糖 (sugar) / 血液 (blood)
128.	田村郁実, 田端厚之, 村上漱, 髙尾亞由子, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus infantis由来の新規コレステロール依存性細胞溶解毒素の作用特性, 第68回日本細菌学会中国・四国支部総会, 2015年10月. (キーワード) 連鎖球菌 (Streptococcus) / 細胞溶解毒素 (cytolysin) / 病原因子 (virulence factor)
129.	友安俊文, 千葉真也, 山崎貴大, 甲斐瑛之, 田端厚之, 長宗秀明 : Streptococcus intermediusのグリコシダーゼによるインターメディリシン発現調節機構の解析, 第56回日本生化学会中国四国支部例会, 2015年5月. (キーワード) 連鎖球菌 (Streptococci) / 酵素 (enzyme) / 糖 (sugar) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
130.	眞屋健太朗, 田端厚之, 大倉一人, 菊池賢, 友安俊文, 長宗秀明 : S. anginosus subsp. whileyi およびS. constellatus subsp. viborgensis が保有するβ溶血因子の特性解析, 第88回日本細菌学会総会, 2015年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 機能 (function)
131.	森下惇, 田端厚之, 村上漱, 高尾亞由子, 大国寿士, 小椋義俊, 林哲也, 友安俊文, 前田伸子, 長宗秀明 : S. mitis におけるコレステロール依存性細胞溶解毒素遺伝子の分布とβ-ラクタム系薬剤耐性の関連性, 第88回日本細菌学会総会, 2015年3月. (キーワード) 連鎖球菌 (Streptococci) / 抗生物質 (antibiotics) / 細胞溶解毒素 (cytolysin)
132.	山崎貴大, 友安俊文, 田端厚之, 長宗秀明 : S. intermedius が保有するグリコシダーゼがインターメディリシン発現制御に果たす役割についての解析, 第88回日本細菌学会総会, 2015年3月. (キーワード) 連鎖球菌 (Streptococci) / 遺伝子発現 (gene expression) / 酵素 (enzyme) / 糖 (sugar)
133.	友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius が保有するシアリダーゼの精製とその性状の解析, 第88回日本細菌学会総会, 2015年3月. (キーワード) 連鎖球菌 (Streptococci) / タンパク質 (protein) / 糖 (sugar)
134.	吉田創, 田端厚之, 友安俊文, 長宗秀明 : Inerolysin (INY) の pH 依存的な膜孔形成活性と立体構造変化, 第133回徳島生物学会総会, 2014年12月. (キーワード) 病原微生物 (pathogenic microorganism) / 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / pH
135.	大久保行将, 安養寺夏希, 田端厚之, 北條恵子, 友安俊文, 立松洋平, 大倉一人, 長宗秀明 : ドラッグデリバリーシステム創成を目指したSortase Aによるペプチド修飾化リポソーム作製技術の開発, 第18回バイオ治療法研究会, 2014年12月. (キーワード) ドラッグデリバリーシステム (drug delivery system) / Sortase A / リポソーム (liposomes)
136.	大久保行将, 安養寺夏希, 田端厚之, 北條恵子, 友安俊文, 長宗秀明 : 新規ドラッグデリバリーシステムツール作製法としてのペプチド修飾化リポソームの作製, 第87回日本生化学会大会, 2014年10月. (キーワード) ドラッグデリバリーシステム (drug delivery system) / リポソーム (liposomes) / ガン治療
137.	玉岡雅章, 竹田望, 田端厚之, 大倉一人, 友安俊文, 大国寿士, 長宗秀明 : コレステロール依存性細胞溶解毒素の膜孔形成活性に対する糖の抑制効果, 第87回日本生化学会大会, 2014年10月. (キーワード) 病原微生物 (pathogenic microorganism) / 連鎖球菌 (Streptococcus) / 病原因子 (virulence factor) / 糖 (sugar) / 細胞溶解毒素 (cytolysin)
138.	安養寺夏希, 田端厚之, 大久保行将, 友安俊文, 長宗秀明 : 細菌由来ペプチド転移酵素Sortase Aの由来菌種依存的な基質認識特性, 第87回日本生化学会大会, 2014年10月. (キーワード) グラム陽性菌 / ブドウ球菌 / 連鎖球菌 (Streptococcus) / Sortase A / 基質特異性
139.	森下惇, 田端厚之, 村上漱, 高尾亞由子, 勝部康弘, 大国寿士, 友安俊文, 前田伸子, 長宗秀明 : Streptococcus mitisにおける膜孔形成毒素遺伝子の保有とβ-ラクタム薬耐性の関連性, 第67回日本細菌学会中国四国支部総会, 2014年10月. (キーワード) 病原微生物 (pathogenic microorganism) / 連鎖球菌 (Streptococcus) / 病原因子 (virulence factor) / 溶血因子 / 薬剤耐性
140.	山崎貴大, 友安俊文, 今木英統, 田端厚之, 長宗秀明 : Streptococcus intermediusの病原性とグリコシダーゼとの関連性, 第67回日本細菌学会中国四国支部総会, 2014年10月. (キーワード) 病原微生物 (pathogenic microorganism) / 連鎖球菌 (Streptococcus) / 糖 (sugar) / グリコシダーゼ
141.	友安俊文, 今木英統, 田上千遥, 山本直輝, 田端厚之, 長宗秀明 : Streptococcus intermedius が保有するグリコシダーゼの病原性に果たす役割について, 第61 回トキシンシンポジウム ∼鳴門渦潮カンファレンス∼, 2014年9月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 糖 (sugar) / グリコシダーゼ
142.	田端厚之, 眞屋健太郎, 大谷浩美, 佐藤裕士, 中野晃太, 大倉一人, 菊池賢, 友安俊文, 長宗秀明 : β溶血性アンギノーサス群レンサ球菌が保有するストレプトリジンSホモログの多様性, 第46回レンサ球菌研究会, 2014年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) / 溶血因子
143.	田端厚之, 竹田望, 玉岡雅章, 友安俊文, 高尾亞由子, 前田伸子, 大国寿士, 長宗秀明 : コレステロール依存性細胞溶解毒素の膜孔形成活性に及ぼす糖の影響, 第55回日本生化学会中国・四国支部例会, 2014年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) / 糖 (sugar)
144.	友安俊文, 今木英統, 田上千遥, 山本直輝, 田端厚之, 長宗秀明 : Streptococcus intermediusが保有する多基質酵素``MsgA''の性状解析, 第55回日本生化学会中国・四国支部例会, 2014年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) / 糖鎖 / グリコシダーゼ
145.	友安俊文, 金子幸広, 田端厚之, 長宗秀明 : Identification and study of the binding region of ily transcriptional repressive element, 第87回日本細菌学会総会, 2014年3月. (キーワード) 連鎖球菌 (Streptococci) / 転写抑制因子結合部位 / 遺伝子発現 (gene expression) / 細胞溶解毒素 (cytolysin)
146.	村上漱, 田端厚之, 髙尾亞由子, 大国寿士, 友安俊文, 長宗秀明 : 高病原性Streptococcus mitisの検出・同定を目的とした新規検査システムの開発, 第87回日本細菌学会総会, 2014年3月. (キーワード) 連鎖球菌 (Streptococci) / 同定 (identification) / PCR / 細胞溶解毒素 (cytolysin)
147.	山本直輝, 友安俊文, 今木英統, 田端厚之, 長宗秀明 : Characterization of a novel secreted glycosidase (MsgA) of Streptococcus intermedius, 第87回日本細菌学会総会, 2014年3月. (キーワード) 連鎖球菌 (Streptococci) / グリコシダーゼ / 糖鎖 / 分解 (decomposition)
148.	安養寺夏希, 田端厚之, 大久保行将, 友安俊文, 長宗秀明 : Comparison of the transition activity of Sortase A using GFP-substrate panel, 第87回日本細菌学会総会, 2014年3月. (キーワード) 酵素 (enzyme) / ペプチド転位酵素 / GFP
149.	金惠珍, 田端厚之, 友安俊文, 上野友美, 内山成人, 湯浅恵造, 辻明彦, 長宗秀明 : エストロゲン作用物質が示す骨芽細胞分化促進作用の解析, 徳島生物学会, 2013年12月. (キーワード) エストロゲン / 骨芽細胞分化
150.	大倉一人, 川口遊喜, 田端厚之, 長宗秀明 : Streptococcus anginosus由来ストレプトリジンSホモログの分子内環形成による活性制御, 第17回バイオ治療法研究会学術集会, 2013年12月. (キーワード) 細菌 (bacteria) / ストレプトリジンS / Streptococcus anginosus
151.	田端厚之, 安養寺夏希, 大久保行将, 友安俊文, 長宗秀明 : 細菌由来ペプチド転移酵素Sortase Aを用いたDDS用新規機能性リポソームの創成に関する検討, 第17回バイオ治療法研究会学術集会, 2013年12月. (キーワード) 細菌 (bacteria) / ドラッグデリバリーシステム (drug delivery system) / リポソーム (liposomes) / ペプチド転移酵素 / GFP
152.	友安俊文, 今木英統, 山本直輝, 田端厚之, 長宗秀明 : Identification and characterization of MsgA, a novel secreted glycosidase of Streptococcus intermedius, 第36回日本分子生物学会年会, 2013年12月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 糖 (sugar) / 酵素 (enzyme)
153.	村上漱, 田端厚之, 髙尾亞由子, 友安俊文, 大国寿士, 長宗秀明 : 高病原性Streptococcus mitis検出・同定システムの構築, 第66回日本細菌学会中国・四国支部総会, 2013年10月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 同定 (identification) / PCR
154.	山本直輝, 友安俊文, 今木英統, 田端厚之, 長宗秀明 : Streptococcus intermediusが分泌する新規グリコシダーゼの発見と性状解析, 第66回日本細菌学会中国・四国支部総会, 2013年10月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 糖 (sugar) / 酵素 (enzyme)
155.	安養寺夏希, 田端厚之, 大久保行将, 友安俊文, 長宗秀明 : 細菌由来ペプチド転移酵素Sortase Aの比較解析 -新規DDS開発への応用を目指して-, 日本生化学会, 2013年9月. (キーワード) 細菌 (bacteria) / ドラッグデリバリーシステム (drug delivery system) / 蛍光タンパク質 / ペプチド転移酵素
156.	竹田望, 田端厚之, 野上明日香, 髙尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : N末に追加ドメインを有するコレステロール依存性細胞溶解毒素の作用特性, 日本生化学会, 2013年9月. (キーワード) 細胞溶解毒素 (cytolysin) / タンパク質 (protein) / コレステロール (cholesterol) / 糖 (sugar)
157.	田端厚之, 大倉一人, 友安俊文, 大国寿士, 長宗秀明 : コレステロール依存性細胞溶解毒素の受容体認識および作用特性における多様性, 第60回毒素シンポジウム, 2013年7月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 遺伝子発現 (gene expression) / 病原因子 (virulence factor)
158.	友安俊文, 今木英統, 山本直輝, 田端厚之, 長宗秀明 : Streptococcus intermedius の主要病原因子であるインターメディリシンの発現調節機構の解析, 第60回毒素シンポジウム, 2013年7月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 遺伝子発現 (gene expression) / 病原因子 (virulence factor)
159.	田端厚之, 中野晃太, 大倉一人, 菊池賢, 友安俊文, 長宗秀明 : β溶血性 Streptococcus anginosus が保有するストレプトリジンS ホモログの特徴と細胞障害性への寄与, 第22回 Lancefieldレンサ球菌研究会, 2013年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor)
160.	今木英統, 友安俊文, 増田早智子, 山本直輝, 田端厚之, 長宗秀明 : 糖タンパク質によるStreptococcus intermediusのily発現促進機構の解析, 第22回 Lancefieldレンサ球菌研究会, 2013年6月. (キーワード) 連鎖球菌 (Streptococcus) / 病原微生物 (pathogenic microorganism) / 病原因子 (virulence factor) / 遺伝子発現 (gene expression)
161.	友安俊文, 近藤博之, 娚杉昌典, 田端厚之, 長宗秀明 : グラム陽性菌と陰性菌のDnaKシャペロンシステムの比較, 第54回日本生化学会中国・四国支部例会, 2013年6月. (キーワード) DnaK / ClpB / グラム陽性菌 / グラム陰性菌 / 分子シャペロン (molecular chaperone)
162.	野上明日香, 田端厚之, 竹田望, 高尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : β溶血性の高病原性Streptococcus mitisサブグループの特性, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 臨床検査 (clinical laboratory testing)
163.	金子幸広, 友安俊文, 今木英統, 田端厚之, 長宗秀明 : ily転写抑制因子の結合領域の探索, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
164.	田端厚之, 佐藤裕士, 中野晃太, 大倉一人, 菊池賢, 友安俊文, 長宗秀明 : S. constellatus subsp. constellatusが保有するβ溶血因子の探索, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 酵素 (enzyme)
165.	竹田望, 田端厚之, 野上明日香, 高尾亞由子, 大国寿士, 前田伸子, 友安俊文, 長宗秀明 : 新規5ドメイン型コレステロール依存性細胞溶解毒素の分子特性, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 酵素 (enzyme)
166.	石川未来, 田端厚之, 友安俊文, 長宗秀明 : ヒト特異的な膜孔形成毒素インターメディリシンにより好中球に誘導される応答反応, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 細胞感染
167.	今木英統, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusのily発現促進因子の同定, 第86回日本細菌学会総会, 2013年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
168.	友安俊文, 田端厚之, 長宗秀明 : グラム陽性菌のDnaKは大腸菌のClpBを認識するのか?, 第86回日本細菌学会総会, 2013年3月. (キーワード) 分子シャペロン (molecular chaperone) / 連鎖球菌 (Streptococcus) / 酵素 (enzyme)
169.	長瀧健司, 友安俊文, 田端厚之, 長宗秀明 : セルラーゼ活性の向上法とその応用についての検討, 日本生化学会, 2012年12月. (キーワード) 分子シャペロン (molecular chaperone) / セルラーゼ / バイオエタノール / 応用 (application)
170.	友安俊文, 田端厚之, 長宗秀明 : 小型分子シャペロンのAgsAは効率的に酵素活性を安定化する, 日本生化学会, 2012年12月. (キーワード) 分子シャペロン (molecular chaperone) / sHSP / 酵素 (enzyme)
171.	金惠珍, 田端厚之, 友安俊文, 上野友美, 内山成人, 湯浅恵造, 辻明彦, 長宗秀明 : エストロゲン刺激で誘導される骨芽細胞の分化機構に関する研究, 日本生化学会, 2012年12月. (キーワード) 骨芽細胞 (osteoblast) / エストロゲン / イソフラボン
172.	大倉一人, 川口遊喜, 田端厚之, 篠原康雄, 長宗秀明 : 細菌由来細胞溶解毒素のヒト側受容体との相互作用, 第16回バイオ治療法研究会学術集会, 2012年12月.
173.	青栁愛美, 田端厚之, 友安俊文, 長宗秀明 : インターメディリシンの細胞膜結合能と分子集合メカニズムの解析, 徳島生物学会, 2012年12月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 細胞膜
174.	岩野泰宜, 田端厚之, 友安俊文, 長宗秀明 : インターメディリシン刺激が単球系細胞THP-1の遺伝子発現に及ぼす影響, 徳島生物学会, 2012年12月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
175.	娚杉昌典, 友安俊文, 田端厚之, 長宗秀明 : 大腸菌のClpBは連鎖球菌のDnaKシャペロンシステムを認識するのか, 徳島生物学会, 2012年12月. (キーワード) 分子シャペロン (molecular chaperone) / Hsp70 / Hsp104 / DnaK / ClpB
176.	竹田望, 田端厚之, 高尾亞由子, 大国寿士, 友安俊文, 田端厚之 : Streptococcus mitis が産生する新規5ドメイン型コレステロール依存性細胞溶解毒素の作用特性, 日本細菌学会, 2012年10月. (キーワード) 連鎖球菌 (Streptococci) / Streptococcus mitis / コレステロール依存性細胞溶解毒素
177.	金子幸広, 友安俊文, 今木英統, 田端厚之, 長宗秀明 : ily 発現抑制因子が認識するコンセンサス配列の探索, 日本細菌学会, 2012年10月. (キーワード) 連鎖球菌 (Streptococci) / 遺伝子発現 (gene expression) / コンセンサス配列
178.	田端厚之, 中野晃太, 大倉一人, 友安俊文, 菊池賢 : アンギノーサス群連鎖球菌が保有するβ溶血因子の探索とその特性, 第59回毒素シンポジウム, 2012年8月.
179.	田端厚之, 中野晃太, 大倉一人, 友安俊文, 菊池賢, 長宗秀明 : β溶血性Streptococcus anginosusが保有するペプチド性溶血因子の分子特性, 第6回細菌学若手コロッセウム, 2012年8月.
180.	金惠珍, 田端厚之, 友安俊文, 上野友美, 内山成人, 長宗秀明 : Effect of Equol, an Enterobacterial Metabolite of Soybean Isoflavones on Osteoblast Differentiation., 第16回腸内細菌学会, 2012年6月.
181.	今木英統, 友安俊文, 増田早智子, 田端厚之, 長宗秀明 : LacRはStreptococcus intermediusのily発現の主要な制御因子である, 第21回 Lancefieldレンサ球菌研究会, 2012年6月.
182.	田端厚之, 野上明日香, 竹田望, 髙尾亞由子, 大国寿士, 友安俊文, 長宗秀明 : コレステロール依存性細胞溶解毒素遺伝子を保有するStreptococcus mitis株の毒素産生特性とヒト細胞障害性, 第21回 Lancefieldレンサ球菌研究会, 2012年6月.
183.	金惠珍, 田端厚之, 友安俊文, 上野友美, 内山成人, 湯浅恵造, 辻明彦, 長宗秀明 : エストロゲン作用物質が示す骨芽細胞の分化促進作用, 第53回日本生化学会中国・四国支部例会, 2012年5月.
184.	友安俊文, 今木英統, 田端厚之, 長宗秀明 : グラム陽性菌と陰性菌のコシャペロンによるDnaK活性化機構の比較, 第85回日本細菌学会総会, 2012年3月. (キーワード) 連鎖球菌 (Streptococci) / 分子シャペロン (molecular chaperone) / タンパク質 (protein)
185.	今木英統, 友安俊文, 増田早智子, 田端厚之, 長宗秀明 : ILYを高産生するStreptococcus intermediusの臨床分離株の多くがLacRに変異を持つ, 第85回日本細菌学会総会, 2012年3月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
186.	田端厚之, 中野晃太, 友安俊文, 大倉一人, 菊池賢, 長宗秀明 : TOMM family peptides decide the hemolytic character of Streptococcus anginosus, 第85回日本細菌学会総会, 2012年3月. (キーワード) 連鎖球菌 (Streptococci) / 細菌毒素 (bacterial toxins)
187.	福居孝之, 田端厚之, 友安俊文, 長宗秀明 : コレステロール依存性細胞溶解毒素の膜孔形成反応における分子動態の解析, 第127回徳島生物学会, 2011年12月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
188.	田端厚之, 大久保行将, 友安俊文, 長宗秀明 : 膜孔形成性キメラ毒素の新規DDSツールとしての応用性に関する検討, 第15回バイオ治療法研究会学術集会, 2011年12月. (キーワード) 癌治療 (cancer therapy)
189.	友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusが保有するDnaKシャペロンシステムの機能解析, 第64回日本細菌学会中国・四国支部総会, 2011年10月. (キーワード) 連鎖球菌 (Streptococci) / 分子シャペロン (molecular chaperone)
190.	中野晃太, 田端厚之, 友安俊文, 大倉一人, 菊池賢, 平松啓一, 長宗秀明 : β溶血性Streptococcus anginosusで見出された新規ペプチド性溶血因子, 第64回日本細菌学会中国・四国支部総会, 2011年10月. (キーワード) 連鎖球菌 (Streptococci) / 細菌毒素 (bacterial toxins)
191.	今木英統, 友安俊文, 増田早智子, 田端厚之, 長宗秀明 : Streptococcus intermediusのily発現調節因子の探索, 第64回日本細菌学会中国・四国支部総会, 2011年10月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin) / 遺伝子発現 (gene expression)
192.	大久保行将, 田端厚之, 田村仁人, 友安俊文, 長宗秀明 : 細胞標的化キメラ細菌毒素を用いた新規DDSツールの開発, 第84回日本生化学会大会, 2011年9月. (キーワード) 癌治療 (cancer therapy)
193.	岡久奈緒美, 田端厚之, 友安俊文, 長宗秀明 : キノコβ-グルカンによるマクロファージ様THP-1の活性化作用, 第84回日本生化学会大会, 2011年9月. (キーワード) 癌免疫療法 (cancer immunotherapy)
194.	田端厚之, 中野晃太, 友安俊文, 長宗秀明 : アンギノーサス群連鎖球菌が保有する溶血因子に関する検討, 第5回細菌学若手コロッセウム, 2011年8月. (キーワード) 連鎖球菌 (Streptococci) / 細菌毒素 (bacterial toxins)
195.	友安俊文, 今木英統, 増田早智子, 田端厚之, 長宗秀明 : コレステロール依存性細胞溶解毒素intermedilysinの発現調節因子がStreptococcus intermediusの病原性に果たす役割の解析, 第58回トキシンシンポジウム, 2011年7月. (キーワード) 連鎖球菌 (Streptococci) / 細胞溶解毒素 (cytolysin)
196.	田端厚之, 中野晃太, 友安俊文, 長宗秀明 : アンギノーサス群連鎖球菌が保有する溶血因子の探索, 第20回Lancefieldレンサ球菌研究会/第43回レンサ球菌感染症研究会, 2011年6月.
197.	友安俊文, 今木英統, 田端厚之, 長宗秀明 : カタボライト抑制因子CcpAによるStreptococcus intermediusのヒト特異的細胞溶解毒素intermedilysinの発現調節, 第33回日本分子生物学会年会第83回日本生化学会大会合同大会, 2010年12月.
198.	田端厚之, 山本泰裕, 友安俊文, 大国寿士, 長宗秀明 : 2種のコレステロール依存性細胞溶解毒素を産生するStreptococcus mitis Nm-65株の細胞障害性に関する検討, 第33回日本分子生物学会年会第83回日本生化学会大会合同大会, 2010年12月.
199.	田村仁人, 田端厚之, 友安俊文, 長宗秀明 : 細菌由来タンパク質をベースとしたDDSツールの開発-標的細胞に対する選択的送達能に関する検討-, 第125回徳島生物学会, 2010年12月.
200.	村田貴洋, 田端厚之, 友安俊文, 長宗秀明 : コレステロール依存性細胞溶解毒素による宿主細胞の応答反応の検討, 第125回徳島生物学会, 2010年12月.
201.	田端厚之, 中野晃太, 友安俊文, 長宗秀明 : Streptococcus anginosusが保有する細胞傷害因子に関する検討, 第63回日本細菌学会中国・四国支部総会, 2010年10月.
202.	増田早智子, 友安俊文, 田端厚之, 長宗秀明 : 血液成分によるily遺伝子の発現調節, 第63回日本細菌学会中国・四国支部総会, 2010年10月.
203.	山本泰裕, 田端厚之, 友安俊文, 弘田克彦, 大国寿士, 長宗秀明 : 川崎病患児由来Streptococcus mitis Nm-65株が産生する2種類のコレステロール依存性細胞溶解毒素遺伝子の発現とその病原性への寄与, 第57回トキシンシンポジウム, 2010年7月.
204.	田端厚之, 鈴木圭祐, 友安俊文, 長宗秀明 : 細胞膜構造に依存したインターメディリシンの作用特性, 第51回日本生化学会中国・四国支部例会, 2010年5月.
205.	今木英統, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermedius ccpA破壊株とcre変異株の細胞毒性の解析, 第83回日本細菌学会総会, 2010年3月. (キーワード) 病原因子 (virulence factor)
206.	友安俊文, 今木英統, 田端厚之, 長宗秀明 : Streptococcus intermedius dnaK破壊相補株の表現型の解析, 第83回日本細菌学会総会, 2010年3月. (キーワード) 病原因子 (virulence factor)
207.	山本泰裕, 田端厚之, 友安俊文, 弘田克彦, 三宅洋一郎, 長宗秀明, 大國寿士 : 2種類のコレステロール依存性細胞溶解毒素遺伝子を持つ新奇Streptococcus mitis株の解析, 第83回日本細菌学会総会, 2010年3月.
208.	Toshifumi Tomoyasu, Atsushi Tabata and Hideaki Nagamune : Dissection of the chaperone function of the small heat shock protein AgsA, 第32回日本分子生物学会年会, Dec. 2009.
209.	田端厚之, 金惠珍, 友安俊文, 高麗寛紀, 澤田千恵子, 下野生世, 菅井基行, 赤松優, 長宗秀明 : 融合PCRイムノクロマトグラフィーによる迅速微生物検査系の構築, 日本防菌防黴学会 2009年度若手の会, 2009年12月.
210.	田端厚之, 三好宗一郎, 天河崇, 友安俊文, 長宗秀明 : 脂質ラフトを反応場とするStreptococcus intermediusのヒト細胞感染機構, 第82回日本生化学会大会, 2009年10月.
211.	垣口貴沙, 田端厚之, 友安俊文, 長宗秀明 : ヒト特異的細胞溶解毒素の膜貫入領域が膜孔形成反応において果たす機能の解析, 第82回日本生化学会大会, 2009年10月.
212.	鈴木圭祐, 田端厚之, 友安俊文, 長宗秀明 : 多様な受容体認識性を示すコレステロール依存性細胞溶解毒素の細胞膜構造に依存した作用特性, 第62回日本細菌学会中国・四国支部総会, 2009年10月.
213.	伊藤貴洋, 田端厚之, 友安俊文, 長宗秀明 : 蛍光性Streptococcus intermedius株の作製, 第62回日本細菌学会中国・四国支部総会, 2009年10月.
214.	田端厚之, 鈴木圭祐, 友安俊文, 大国寿士, 長宗秀明 : 標的細胞膜構造がコレステロール依存性細胞溶解毒素の受容体認識多様性に及ぼす影響, 若手研究者育成のためのワークショップ若手コロセウム(III), 2009年10月.
215.	友安俊文, 田端厚之, 菊池賢, 佐々木崇, 馬場理, 平松啓一, 長宗秀明 : Streptococcus intermediusが産生するヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第56回トキシンシンポジウム, 106-109, 2009年8月.
216.	友安俊文, 廣島理樹, 今木英統, 田端厚之, 長宗秀明 : Streptococcus intermediusのily発現調節機構の解析, 第18回 Lancefield レンサ球菌研究会, 2009年6月.
217.	田端厚之, 篠原由樹, 鈴木圭祐, 友安俊文, 長宗秀明 : インターメディリシンの膜孔形成に携わる分子構造と機能に関する解析, 第18回 Lancefieldレンサ球菌研究会, 2009年6月.
218.	田端厚之, 篠原由樹, 友安俊文, 長宗秀明 : インターメディリシンの分子間相互作用に寄与する部分構造の解析, 第50回日本生化学会中国・四国支部例会, 2009年5月.
219.	友安俊文, 田端厚之, 菊池賢, 佐々木崇, 馬場理, 平松啓一, 長宗秀明 : カタボライト抑制因子CcpAによるヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第82回日本細菌学会総会, 2009年3月.
220.	垣口貴沙, 田端厚之, 友安俊文, 長宗秀明 : ヒト特異的な細胞溶解毒素インターメディリシン(ILY)の膜孔形成を誘導する構造変化領域の解析, 第82回日本日本細菌学会総会, 2009年3月.
221.	金惠珍, 友安俊文, 田端厚之, 菅井基行, 赤松優, 長宗秀明 : 融合PCRイムノクロマトグラフィーによる迅速細菌検査, 第82回日本細菌学会総会, 2009年3月.
222.	友安俊文, 田端厚之, 長宗秀明 : Streptooccus intermediusのストレス誘導型シャペロンDnaKの機能解析, 第82回日本日本細菌学会総会, 2009年3月.
223.	弘田克彦, 鹿山鎭男, 湯本浩通, 田端厚之, 長宗秀明, 小野恒子, 三宅洋一郎 : Intermedilysinにより誘導される小胞体異常とgp210の細胞内分布の変動, 第82回日本日本細菌学会総会, 2009年3月.
224.	齋藤静香, 田端厚之, 坂倉永里子, 鈴木圭祐, 垣口貴沙, 篠原由樹, 福島江, 友安俊文, 大国寿士, 長宗秀明 : コレステロール依存性細胞溶解毒素の受容体認識特性に関する検討, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月.
225.	坂倉永里子, 田端厚之, 齋藤静香, 友安俊文, 長宗秀明 : 癌標的化毒素を用いた癌療法のための効果的DDSの開発, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月.
226.	廣島理樹, 友安俊文, 小南章, 田端厚之, 菊池賢, 佐々木崇, 馬場理, 平松啓一, 長宗秀明 : Streptococcus intermediusが保有するヒト特異的細胞溶解毒素インターメディリシンをコードするily遺伝子のプロモーター領域の解析, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月.
227.	石川洋子, 友安俊文, 田端厚之, 長宗秀明 : サルモネラ属細菌が保有する小型分子シャペロンAgsAのN末端とC末端領域の機能解析, 第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月.
228.	垣口貴沙, 田端厚之, 友安俊文, 長宗秀明 : ヒト特異的な細胞溶解毒素インターメディリシンの膜孔形成に関与する分子構造の解析, 第120回徳島生物学会, 2008年11月.
229.	弘田克彦, 鹿山鎭男, 田端厚之, 長宗秀明, 小野恒子, 三宅洋一郎 : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死とgp210の細胞内分布の変動, 第61回日本細菌学会中国・四国支部総会, 2008年10月.
230.	田端厚之, 齋藤静香, 坂倉永里子, 鈴木圭祐, 篠原由樹, 福島江, 友安俊文, 大国寿士, 長宗秀明 : コレステロール及びヒト型CD59を受容体とするCholesterol-dependent cytolysin:Sm-hPAFのコレステロール認識特性に関する検討, 第61回日本細菌学会中国・四国支部総会, 2008年10月.
231.	三好宗一郎, 田端厚之, 友安俊文, 長宗秀明 : Streptococcus intermediusのヒト細胞感染現象におけるインターメディリシンと宿主細胞膜構造の役割, 第61回日本細菌学会中国・四国支部総会, 2008年10月.
232.	田端厚之, 友安俊文, 長宗秀明 : 連鎖球菌属が産生するコレステロール依存性細胞溶解毒素の受容体認識における多様性, 第2回細菌学若手コロッセウム, 2008年8月.
233.	廣島理樹, 友安俊文, 田端厚之, 長宗秀明 : ヒト特異的細胞溶解毒素インターメディリシンの発現調節機構の解析, 第17回 Lancefieldレンサ球菌研究会, 2008年7月.
234.	田端厚之, 齋藤静香, 坂倉永里子, 鈴木圭祐, 山本泰裕, 篠原由樹, 福島江, 友安俊文, 大国寿士, 長宗秀明 : ヒトCD59指向性コレステロール依存性細胞溶解毒素の受容体認識特性に関する検討, 第17回 Lancefieldレンサ球菌研究会, 2008年7月.
235.	弘田克彦, Heni Susilowati, 岡村裕彦, 吉田賀弥, 田端厚之, 鹿山鎭男, 湯本浩通, 羽地達次, 長宗秀明, 小野恒子, 三宅洋一郎 : Intermedilysinにより誘導されるカルシウム振動と胆管上皮細胞死, 第17回Lancefielfレンサ球菌研究会 2008, 2008年7月.
236.	石川洋子, 友安俊文, 田端厚之, 長宗秀明 : サルモネラ属細菌が保有する小型分子シャペロンAgsAの機能解析, 第49回日本生化学会中国・四国支部例会, 2008年5月.
237.	垣口貴沙, 田端厚之, 友安俊文, 長宗秀明 : ヒト特異的な細胞溶解毒素インターメディリシン(ILY)の膜孔形成に関わる部分構造に関する解析, 第49回日本生化学会中国・四国支部例会, 2008年5月.
238.	田端厚之, 三好宗一郎, 友安俊文, 長宗秀明 : 宿主細胞膜構造がStreptococcus intermediusのヒト細胞感染に及ぼす影響, 第81回日本細菌学会総会, 2008年3月. (キーワード) 連鎖球菌 / 脂質ラフト / 感染
239.	長宗秀明, 友安俊文, 田端厚之 : ヒト特異的な細胞溶解毒素インターメディリシンの発現調節の遺伝学的解析, 第81回日本細菌学会総会, 2008年3月.
240.	弘田克彦, 鹿山鎭男, 田端厚之, 長宗秀明, 小野恒子, 三宅洋一郎 : Intermedilysinにより誘導される非アポトーシス型細胞死と細胞内Ca²⁺濃度の変動, 第81回日本細菌学会総会, 2008年3月.
241.	友安俊文, 田端厚之, 長宗秀明 : 連鎖球菌(S. intermedius)ClpP破壊株の作成とその表現型の解析, 第81回日本細菌学会総会, 2008年3月. (キーワード) 連鎖球菌 / プロテアーゼ (protease) / ClpP
242.	田端厚之, 三好宗一郎, 角田明由葉, 福島江, 助野晃子, 友安俊文, 弘田克彦, 三宅洋一郎, 大国寿士, 長宗秀明 : 細菌感染反応場としてのヒト型CD59:ヒト細胞指向性コレステロール依存性細胞溶解毒素の受容体及びStreptococcus intermedius感染の宿主側トリガー因子としての役割, 第80回日本生化学会大会-第30回日本分子生物学会年会合同大会, 2007年12月. (キーワード) 連鎖球菌 / CD59 / 感染
243.	廣島理樹, 友安俊文, 田端厚之, 長宗秀明 : Streptococcus intermediusが生産するヒト特異的細胞溶解毒素インターメディシンの発現調節, 第80回日本生化学会大会-第30回日本分子生物学会年会合同大会, 2007年12月. (キーワード) 連鎖球菌 / 遺伝子発現
244.	友安俊文, 東俊介, 今木英統, 田端厚之, 長宗秀明 : 腸管出血性大腸菌のGrlAによるLEE1オペロンの新規発現調節機構, 第30回日本分子生物学会年会, 2007年12月. (キーワード) 腸管出血性大腸菌 / LEE pathgenisity island
245.	弘田克彦, Susilowati Heni, 劉大力, 鹿山鎭男, 田端厚之, 長宗秀明, 小野恒子, 三宅洋一郎 : Intermedilysinにより誘導されるヒト培養胆管上皮細胞死の分子機構の解明, 第60回日本細菌学会中国四国支部総会, 2007年10月.
246.	篠原由樹, 石田巧, 田端厚之, 友安俊文, 長宗秀明 : インターメディリシンの分子間相互作用部位の解析, 第60回日本細菌学会中国四国支部総会, 2007年10月. (キーワード) 連鎖球菌 / 細胞溶解毒素
247.	田端厚之, 三好宗一郎, 角田明由葉, 助野晃子, 弘田克彦, 友安俊文, 三宅洋一郎, 長宗秀明 : Streptococcus intermedius が分泌する細胞溶解毒素インターメディリシンが関与するヒト細胞特異的な感染現象, 第54回毒素シンポジウム, 2007年9月. (キーワード) 細胞毒性 (cytotoxicity) / 連鎖球菌
248.	友安俊文, 廣島理樹, 石田俊輔, 小南章, 田端厚之, 長宗秀明 : Streptococcus intermediusの病原性発現調節機構の解析, 第16回Lancefieldレンサ球菌研究会, 2007年6月. (キーワード) 連鎖球菌 / 病原性 / シャペロン
249.	田端厚之, 三好宗一郎, 友安俊文, 弘田克彦, 三宅洋一郎, 長宗秀明 : 細胞溶解毒素インターメディリシンのヒト細胞特異的感染因子としての役割, 第80回日本細菌学会総会, 2007年3月.
250.	今木英統, 友安俊文, 田端厚之, 長宗秀明 : GrlR，GrlAによる腸管出血性大腸菌(EHEC)のLEE領域発現制御機構の解析, 第80回日本細菌学会総会, 2007年3月.
251.	友安俊文, 田端厚之, 長宗秀明 : 腸管出血性大腸菌(EHEC)のLEE領域発現制御機構の解析, 第59回日本細菌学会中国・四国支部総, 2006年10月.
252.	福島江, 田端厚之, 友安俊文, 大倉一人, 大国壽士, 長宗秀明 : Sm-hPAFの受容体多様性:コレステロール依存性細胞溶解毒素の分子進化, 第59回日本細菌学会中国・四国支部総, 2006年10月.

作品:

1.	田端厚之 : 細胞膜に障害を及ぼすレンサ球菌由来溶血毒素の構造的および機能的な多様性, 生化学6, Vol.95, No.6, 757-764, 2023年12月.
2.	田端厚之 : 溶血毒素産生性アンギノーサス群レンサ球菌とその潜在的病原性, 月刊「細胞」, Vol.54, No.10, 54(588)-57(591), 2022年9月.

その他・研究会:

1.	田端厚之, 宇都義浩, 大倉一人 : UTX-114ファミリーとEGFR-tykとの相互作用解析:アセチルグルコース付加によるゲフィチニブ機能の修飾, 第27回バイオ治療法学会, 2023年12月.
2.	田端厚之, 横畑修人, 大倉一人, 友安俊文, 長宗秀明 : ペプチド溶血毒素ストレプトリジンSの血液成分による安定化と病原性への寄与, 第53回レンサ球菌研究会, 2023年7月.
3.	田端厚之, 大倉一人 : C-配糖体型SGLT2阻害剤の分子特性:ヒトSGLT2モデルとの相互作用解析, 第26回日本バイオ治療法学会学術集会, 2022年12月.
4.	田端厚之, 宇都義浩, 大倉一人 : UTX-51誘導体とモデル標的タンパク質との相互作用:中性子照射による終末糖化産物破壊に寄与するボロントレース化合物の開発, 第25回バイオ治療法研究会学術集会, 2021年12月.
5.	田端厚之, 立松洋平, 大倉一人 : リアルタイム薬剤検出システムの構築試行:ミトコンドリア酸素消費速度を利用した担体からの薬剤放出特性の解析, 第24回バイオ治療法研究会学術集会, 2020年12月.
6.	田端厚之, 宇都義浩, 堀均, 大倉一人 : Chiral-2-nitroimidazole骨格を足場としたTX-2036誘導体の特性:EGF受容体キナーゼドメインとの相互作用, 第23回バイオ治療法研究会学術集会, 2019年12月.
7.	天羽崇, 村上圭史, 田端厚之, 長宗秀明, 弘田克彦, 三宅洋一郎, 藤猪英樹 : 幼児口腔内から検出された多剤耐性レンサ球菌について, 第71回日本細菌学会中国，四国支部総会, 2018年10月.
8.	田端厚之, 安養寺夏希, 大久保行将, 北條恵子, 水品善之, 友安俊文, 長宗秀明 : ナノ粒子の標的化技術としての表面加工システムの開発, --- GFP基質パネルを用いた細菌由来タンパク質転移酵素Sortase Aのペプチド転移反応の至適化とその応用に向けて ---, 神戸学院大学ライフサイエンス産学連携研究センター, 2014年3月.
9.	田端厚之, 大久保行将, 安養寺夏希, 友安俊文, 長宗秀明 : ナノ粒子の標的化技術としての表面加工システムの開発, --- 表面加工システムに用いるタンパク質転移酵素SrtA用の基質化システムの開発と検討 ---, 神戸学院大学ライフサイエンス産学連携研究センター, 2013年3月.
10.	田端厚之, 大久保行将, 友安俊文, 長宗秀明 : 標的細胞に対する汎用性を高めたDDSツールの構築とその機能に関する評価, 第16回バイオ治療法研究会学術集会, 2012年12月.
11.	友安俊文, 岡本歩, 今木英統, 田端厚之, 長宗秀明 : ラクトースリプレッサーLacRによるインターメディリシン遺伝子の発現抑制, 第19回Lancefieldレンサ球菌研究会, 2010年6月.
12.	田端厚之, 山本泰裕, 友安俊文, 弘田克彦, 三宅洋一郎, 大国寿士, 長宗秀明 : 2種のコレステロール依存性細胞溶解毒素を産生する川崎病患児由来Streptococcus mitis Nm-65株の病原性に関する検討, 第19回Lancefieldレンサ球菌研究会, 2010年6月.

報告書:

1.	田端厚之 : 川崎病児由来レンサ球菌が産生するコレステロール依存性細胞溶解毒素による血管炎症惹起メカニズムの解明, 公益財団法人大山健康財団 2023年度年報, Vol.49, 19-22, 2023年12月.

特許:

1.	長宗秀明, 友安俊文, 田端厚之 : コレステロール依存性細胞溶解毒素の変異体及びそのDDSへの利用, 特願WO2012/121395 A1 (2012年9月), .
2.	佐々木健二, 綿矢有佑, 金惠淑, 高麗寛紀, 田端厚之, 難波哲人, 清武義弘, 田村卓二, 吉田宗弘 : 新規抗マラリア剤, 特願2003-419719 (2003年12月), 特開2005-179212 (2005年7月), .

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

バイオマス由来の低分子化リグニンを活用したカビの次世代型光殺菌技術の開発 (研究課題/領域番号: 23K27041 )
アンギノーサス群レンサ球菌が産生するストレプトリジンSに特徴的な分子ダイナミズム (研究課題/領域番号: 23K09139 )
病原性連鎖球菌に由来する細胞傷害因子の構造機能解析と感染制御への応用 (研究課題/領域番号: 21K06655 )
アンギノーサス群レンサ球菌をヒト病原細菌へと変貌させる環境要因は何か？ (研究課題/領域番号: 20K09919 )
口腔連鎖球菌の病原性に関与する溶血毒素の分子進化 (研究課題/領域番号: 18K09552 )
連鎖球菌産生毒素の機能解析と感染症制御への応用 (研究課題/領域番号: 18K06764 )
細胞障害性を示すアンギノーサス群連鎖球菌における可動性遺伝因子の存在意義 (研究課題/領域番号: 17K11615 )
アンギノーサス群連鎖球菌由来ストレプトリジンSホモログに特徴的な分子特性の解析 (研究課題/領域番号: 15K20361 )
細胞溶解毒素遺伝子の伝搬とその発現による口腔連鎖球菌の病原性化機構 (研究課題/領域番号: 15K11012 )
ヒト特異的な病原性連鎖球菌が分泌する新規グリコシダーゼの病原性に果たす役割の解析 (研究課題/領域番号: 26460528 )
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教授 : 田端厚之

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教授 : 田端 厚之

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教授 : 田端厚之