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徳島大学バイオイノベーション研究所
徳島大学研究クラスター群研究クラスター群 (指定)2001001 ゲノム編集技術を活用した異種キメラブタの開発
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専門分野

ライフサイエンス (Life sciences) [動物生命科学 (Zoological sciences)]

研究テーマ

著書・論文

学術論文(審査論文):

1. Naoaki Yoshimura, Masayasu Taniguchi, Tsukasa Terazono, Tetsushi Ono, Mitsuhiro Takagi, Yoko Sato, Maki Hirata, Fuminori Tanihara and Takeshige Otoi :
Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG.,
Veterinary Medicine and Science, 2021.
(DOI: 10.1002/vms3.552,   PubMed: 34137199)
2. Zhao Namula, Yasuhiro Isumi, Yoko Sato, Anh Quynh Le, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok and Takeshige Otoi :
Improvement of the in vitro fertilization and embryo development using frozen-thawed spermatozoa of microminipigs.,
Archives Animal Breeding, Vol.64, No.1, 265-271, 2021.
(徳島大学機関リポジトリ: 116482,   DOI: 10.5194/aab-64-265-2021,   PubMed: 34189254)
3. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi and Takeshige Otoi :
One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.,
International Journal of Molecular Sciences, Vol.22, No.5, 2249, 2021.
(徳島大学機関リポジトリ: 116340,   DOI: 10.3390/ijms22052249,   PubMed: 33668187,   Elsevier: Scopus)
4. Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Le Anh, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara and Takeshige Otoi :
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.,
Animals : An Open Access Journal from MDPI, Vol.11, No.2, 2021.
(徳島大学機関リポジトリ: 116270,   DOI: 10.3390/ani11020578,   PubMed: 33672168,   Elsevier: Scopus)
5. Anh Quynh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata and Takeshige Otoi :
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.,
BMC Research Notes, Vol.14, No.1, 7, 2021.
(徳島大学機関リポジトリ: 116277,   DOI: 10.1186/s13104-020-05412-8,   PubMed: 33407863)
6. Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, T Nhien Nguyen, A Quynh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara and Takeshige Otoi :
Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.,
Animal Science Journal, Vol.92, No.1, e13534, 2021.
(DOI: 10.1111/asj.13534,   PubMed: 33638256)
7. Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Anh Quynh Le, Qingyi Lin, Koki Takebayashi and Takeshige Otoi :
One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.8, 614-621, 2020.
(DOI: 10.1007/s11626-020-00507-9,   PubMed: 32978715)
8. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi :
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes.,
BMC Biotechnology, Vol.20, No.1, 40, 2020.
(徳島大学機関リポジトリ: 115881,   DOI: 10.1186/s12896-020-00638-7,   PubMed: 32811500)
9. Maki Hirata, Manita Wittayarat, Zhao Namula, Anh Quynh Le, Qingyi Lin, Thi Nhien Nguyen, Koki Takebayashi, Yoko Sato, Fuminori Tanihara and Takeshige Otoi :
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation.,
Molecular Biology Reports, Vol.47, No.7, 5073-5079, 2020.
(DOI: 10.1007/s11033-020-05576-3,   PubMed: 32519310)
10. Takayuki Hirano, Maki Hirata, Shigeyuki Fujimoto, Thi Nhien Nguyen, Le Anh Quynh, Fuminori Tanihara and Takeshige Otoi :
Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells.,
In Vitro Cellular & Developmental Biology. Animal, Vol.56, No.4, 277-280, 2020.
(DOI: 10.1007/s11626-020-00451-8,   PubMed: 32394241)
11. Fuminori Tanihara, Maki Hirata, Nhien Nguyen Thi, Le Anh Quynh, Takayuki Hirano and Takeshige Otoi :
Generation of viable PDX1 gene-edited founder pigs as providers of nonmosaics.,
Molecular Reproduction and Development, Vol.87, No.4, 471-481, 2020.
(DOI: 10.1002/mrd.23335,   PubMed: 32166879)
12. Yoko Sato, Ryota Kuriwaki, Shiki Hagino, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi and Takeshige Otoi :
Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.55, No.2, 209-216, 2020.
(DOI: 10.1111/rda.13609,   PubMed: 31858644)
13. A Quynh Le, Maki Hirata, T Nhien Nguyen, Koki Takebayashi, Manita Wittayarat, Yoko Sato, Zhao Namula, Masahiro Nii, Fuminori Tanihara and Takeshige Otoi :
Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes.,
Animal Science Journal, Vol.91, No.1, e13386, 2020.
(DOI: 10.1111/asj.13386,   PubMed: 32512638)
14. Fuminori Tanihara, Maki Hirata, Thi Nhien Nguyen, Le Anh Quynh, Manita Wittayarat, Mokhamad Fahrudin, Takayuki Hirano and Takeshige Otoi :
Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes.,
Animal Biotechnology, 2019.
(DOI: 10.1080/10495398.2019.1668801,   PubMed: 31558095)
15. Maki Hirata, Manita Wittayarat, Takayuki Hirano, Thi Nhien Nguyen, Le Anh Quynh, Zhao Namula, Mokhamad Fahrudin, Fuminori Tanihara and Takeshige Otoi :
The relationship between embryonic development and the efficiency of target mutations in porcine endogenous retroviruses (PERVs) pol genes in porcine embryos.,
Animals : An Open Access Journal from MDPI, Vol.9, No.9, E593, 2019.
(徳島大学機関リポジトリ: 114984,   DOI: 10.3390/ani9090593,   PubMed: 31443357)
16. Namula Zhao, Manita Wittayarat, Maki Hirata, Hirano Takayuki, Nguyen Thi Nhien, Le Anh Quynh, Fahrudin Mokhamad, Fuminori Tanihara and Takeshige Otoi :
Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.8, 598-603, 2019.
(DOI: 10.1007/s11626-019-00385-w,   PubMed: 31297696)
17. Fuminori Tanihara, Maki Hirata, Morikawa Shigeki, Thi Nhien Nguyen, Le Anh Quynh, Hirano Takayuki, Fukumi Yoshiyuki, Abe Toshiaki and Takeshige Otoi :
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts,
The Journal of Reproduction and Development, Vol.65, No.5, 475-479, 2019.
(徳島大学機関リポジトリ: 115540,   DOI: 10.1262/jrd.2019-049,   PubMed: 31178553)
18. Fuminori Tanihara, Maki Hirata, Satoru Iizuka, Shinya Sairiki, Masahiro Nii, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano and Takeshige Otoi :
Relationship among ovarian follicular status, developmental competence of oocytes, and anti-M llerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.,
Animal Science Journal, Vol.90, No.6, 712-718, 2019.
(DOI: 10.1111/asj.13200,   PubMed: 30977253)
19. Nhien Nguyen Thi, Maki Hirata, Fuminori Tanihara, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.54, No.5, 750-755, 2019.
(DOI: 10.1111/rda.13417,   PubMed: 30788874,   Elsevier: Scopus)
20. Zhao Namula, Fuminori Tanihara, Manita Wittayarat, Maki Hirata, Nhien Thi Nguyen, Takayuki Hirano, Quynh Anh Le, Masahiro Nii and Takeshige Otoi :
Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.,
Acta Veterinaria Hungarica, Vol.67, No.1, 106-114, 2019.
(DOI: 10.1556/004.2019.012,   PubMed: 30922097)
21. Maki Hirata, Fuminori Tanihara, Manita Wittayarat, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, Zhao Namula, Masahiro Nii and Takeshige Otoi :
Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.,
In Vitro Cellular & Developmental Biology. Animal, Vol.55, No.4, 237-242, 2019.
(DOI: 10.1007/s11626-019-00338-3,   PubMed: 30820813)
22. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh LE, Takayuki Hirano and Takeshige Otoi :
Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos.,
The Journal of Reproduction and Development, Vol.65, No.3, 209-214, 2019.
(徳島大学機関リポジトリ: 115541,   DOI: 10.1262/jrd.2018-116,   PubMed: 30726783,   Elsevier: Scopus)
23. Fuminori Tanihara, Maki Hirata, Nhien T. Nguyen, Quynh A. Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.,
Animal Science Journal, Vol.90, No.1, 55-61, 2018.
(DOI: 10.1111/asj.13129,   PubMed: 30368976)
24. Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Quynh Anh Le, Takayuki Hirano, Tatsuya Takemoto, Michiko Nakai, Dai-Ichiro Fuchimoto and Takeshige Otoi :
Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.,
PLoS ONE, Vol.13, No.10, e0206360, 2018.
(徳島大学機関リポジトリ: 112917,   DOI: 10.1371/journal.pone.0206360,   PubMed: 30352075)
25. Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Mitsuhiro Takagi, Tsukasa Terazono and Takeshige Otoi :
Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.,
Veterinary Medicine and Science, 2018.
(徳島大学機関リポジトリ: 115827,   DOI: 10.1002/vms3.121,   PubMed: 30273973)
26. Maki Hirata, Fuminori Tanihara, Taniguchi Masayasu, Takagi Mitsuhiro, Terazono Tsukasa and Takeshige Otoi :
Viability of canine ovaries autografted to different peripheral sites,
The International Journal of Applied Research in Veterinary Medicine, Vol.16, No.2, 140-148, 2018.
27. Zhao Namula, Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Nhien Nguyen Thi, Takayuki Hirano, Masahiro Nii and Takeshige Otoi :
Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.,
Reproduction in Domestic Animals = Zuchthygiene, 2018.
(徳島大学機関リポジトリ: 116076,   DOI: 10.1111/rda.13288,   PubMed: 30053311)
28. Thanh-Van Nguyen, Manita Wittayarat, Kim Lanh Thi Do, Van Thanh Nguyen, Masahiro Nii, Zhao Namula, Toshiki Kunihara, Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.,
Animal Science Journal, Vol.89, No.8, 1207-1213, 2018.
(徳島大学機関リポジトリ: 116078,   DOI: 10.1111/asj.13049,   PubMed: 29806122)
29. Fuminori Tanihara, Maki Hirata, Thi Nguyen Nhien, Takayuki Hirano, Toshiki Kunihara and Takeshige Otoi :
Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation.,
The Journal of Veterinary Medical Science, Vol.80, No.6, 1007-1011, 2018.
(徳島大学機関リポジトリ: 115574,   DOI: 10.1292/jvms.18-0131,   PubMed: 29769451)
30. V T Nguyen, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Katsutoshi Nishio, T Do L Kim, V T Nguyen, M Nii and Takeshige Otoi :
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.,
Cryo Letters, Vol.39, No.2, 131-136, 2018.
(PubMed: 29734422,   Elsevier: Scopus)
31. Katsutoshi Nishio, Fuminori Tanihara, T-V Nguyen, Toshiki Kunihara, M Nii, Maki Hirata, Tatsuya Takemoto and Takeshige Otoi :
Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.,
Reproduction in Domestic Animals = Zuchthygiene, Vol.53, No.2, 313-318, 2017.
(徳島大学機関リポジトリ: 116077,   DOI: 10.1111/rda.13106,   PubMed: 29135047)
32. Nobuyuki Nakajima, Tetsuro Tamaki, Maki Hirata, Shuichi Soeda, Masahiro Nitta, Akio Hoshi and Toshiro Terachi :
Purified human skeletal muscle-derived stem cells enhance the repair and regeneration in the damaged urethra.,
Transplantation, Vol.101, No.10, 2312-2320, 2017.
(DOI: 10.1097/TP.0000000000001613,   PubMed: 28027190)
33. Tetsuro Tamaki, Maki Hirata, Nobuyuki Nakajima, Kosuke Saito, Hiroyuki Hashimoto, Shuichi Soeda, Yoshiyasu Uchiyama and Masahiko Watanabe :
A long-gap peripheral nerve injury therapy using human skeletal muscle-derived stem cells (Sk-SCs): An achievement of significant morphological, numerical and functional recovery.,
PLoS ONE, Vol.11, No.11, e0166639, 2016.
(徳島大学機関リポジトリ: 115242,   DOI: 10.1371/journal.pone.0166639,   PubMed: 27846318)
34. Hiroyuki Hashimoto, Tetsuro Tamaki, Maki Hirata, Yoshiyasu Uchiyama, Masato Sato and Joji Mochida :
Reconstitution of the complete rupture in musculotendinous junction using skeletal muscle-derived multipotent stem cell sheet-pellets as a "bio-bond".,
PeerJ, Vol.4, e2231, 2016.
(DOI: 10.7717/peerj.2231,   PubMed: 27547541)
35. Kosuke Saito, Tetsuro Tamaki, Maki Hirata, Hiroyuki Hashimoto, Kenei Nakazato, Nobuyuki Nakajima, Akihito Kazuno, Akihiro Sakai, Masahiro Iida and Kenji Okami :
Reconstruction of multiple facial nerve branches using skeletal muscle-derived multipotent stem cell sheet-pellet transplantation.,
PLoS ONE, Vol.10, No.9, e0138371, 2015.
(DOI: 10.1371/journal.pone.0138371,   PubMed: 26372044)
36. Tetsuro Tamaki, Yoshiyasu Uchiyama, Maki Hirata, Hiroyuki Hashimoto, Nobuyuki Nakajima, Kosuke Saito, Toshiro Terachi and Joji Mochida :
Therapeutic isolation and expansion of human skeletal muscle-derived stem cells for the use of muscle-nerve-blood vessel reconstitution.,
Frontiers in Physiology, Vol.6, 165, 2015.
(DOI: 10.3389/fphys.2015.00165,   PubMed: 26082721)
37. Tetsuro Tamaki, Maki Hirata and Yoshiyasu Uchiyama :
Qualitative alteration of peripheral motor system begins prior to appearance of typical sarcopenia syndrome in middle-aged rats.,
Frontiers in Aging Neuroscience, Vol.6, 296, 2014.
(DOI: 10.3389/fnagi.2014.00296,   PubMed: 25400579)
38. Tetsuro Tamaki, Maki Hirata, Shuichi Soeda, Nobuyuki Nakajima, Kosuke Saito, Kenei Nakazato, Yoshinori Okada, Hiroyuki Hashimoto, Yoshiyasu Uchiyama and Joji Mochida :
Preferential and comprehensive reconstitution of severely damaged sciatic nerve using murine skeletal muscle-derived multipotent stem cells.,
PLoS ONE, Vol.9, No.3, e91257, 2014.
(DOI: 10.1371/journal.pone.0091257,   PubMed: 24614849)
39. Tetsuro Tamaki, Shuichi Soeda, Hiroyuki Hashimoto, Kosuke Saito, Akihiro Sakai, Nobuyuki Nakajima, Maki Masuda, Nahoko Fukunishi, Yoshiyasu Uchiyama, Toshiro Terachi and Joji Mochida :
3D reconstitution of nerve-blood vessel networks using skeletal muscle-derived multipotent stem cell sheet pellets.,
Regenerative Medicine, Vol.8, No.4, 437-451, 2013.
(DOI: 10.2217/rme.13.30,   PubMed: 23826698)
40. Tetsuro Tamaki, Kayoko Tono, Yoshiyasu Uchiyama, Yoshinori Okada, Maki Masuda, Shuichi Soeda, Masahiro Nitta and Akira Akatsuka :
Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro.,
Cell and Tissue Research, Vol.344, No.1, 147-168, 2011.
(DOI: 10.1007/s00441-010-1127-9,   PubMed: 21274567)
41. Masahiro Nitta, Tetsuro Tamaki, Kayoko Tono, Yoshinori Okada, Maki Masuda, Akira Akatsuka, Akio Hoshi, Yukio Usui and Toshiro Terachi :
Reconstitution of experimental neurogenic bladder dysfunction using skeletal muscle-derived multipotent stem cells.,
Transplantation, Vol.89, No.9, 1043-1049, 2010.
(DOI: 10.1097/TP.0b013e3181d45a7f,   PubMed: 20150836)
42. Tetsuro Tamaki, Yoshiyasu Uchiyama, Yoshinori Okada, Kayoko Tono, Maki Masuda, Masahiro Nitta, Akio Hoshi and Akira Akatsuka :
Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo.,
Stem Cells and Development, Vol.19, No.4, 503-512, 2010.
(DOI: 10.1089/scd.2009.0179,   PubMed: 19634996)
43. Kazunari Yamashita, Atsushi Suzuki, Yoshinori Satoh, Mariko Ide, Yoshiko Amano, Maki Masuda-Hirata, K Yukiko Hayashi, Keisuke Hamada, Kazuhiro Ogata and Shigeo Ohno :
The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b.,
Biochemical and Biophysical Research Communications, Vol.391, No.1, 812-817, 2009.
(DOI: 10.1016/j.bbrc.2009.11.144,   PubMed: 19945424)
44. Maki Masuda-Hirata, Atsushi Suzuki, Yoshiko Amano, Kazunari Yamashita, Mariko Ide, Tomoyuki Yamanaka, Michihiro Sakai, Michihiro Imamura and Shigeo Ohno :
Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex.,
Genes to Cells, Vol.14, No.7, 835-850, 2009.
(DOI: 10.1111/j.1365-2443.2009.01315.x,   PubMed: 19549170)
45. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Masahiro Nitta, Akio Hoshi and Akira Akatsuka :
Skeletal muscle-derived CD34+/45- and CD34-/45- stem cells are situated hierarchically upstream of Pax7+ cells.,
Stem Cells and Development, Vol.17, No.4, 653-667, 2008.
(DOI: 10.1089/scd.2008.0070,   PubMed: 18554087)
46. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Mika Wada, Akio Hoshi and Akira Akatsuka :
Synchronized reconstitution of muscle fibers, peripheral nerves and blood vessels by murine skeletal muscle-derived CD34(-)/45 (-) cells.,
Histochemistry and Cell Biology, Vol.128, No.4, 349-360, 2007.
(DOI: 10.1007/s00418-007-0331-5,   PubMed: 17762938)
47. Tetsuro Tamaki, Yoshinori Okada, Yoshiyasu Uchiyama, Kayoko Tono, Maki Masuda, Mika Wada, Akio Hoshi, Tetsuya Ishikawa and Akira Akatsuka :
Clonal multipotency of skeletal muscle-derived stem cells between mesodermal and ectodermal lineage.,
Stem Cells, Vol.25, No.9, 2283-2290, 2007.
(DOI: 10.1634/stemcells.2006-0746,   PubMed: 17588936)
48. Atsushi Suzuki, Maki Hirata, Katsusi Kamimura, Rika Maniwa, Tomoyuki Yamanaka, Keiko Mizuno, Masaru Kishikawa, Hiroko Hirose, Yoshiko Amano, Natsuko Izumi, Yoshihiro Miwa and Shigeo Ohno :
aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity.,
Current Biology, Vol.14, No.16, 1425-1435, 2004.
(DOI: 10.1016/j.cub.2004.08.021,   PubMed: 15324659)
49. Mitsuhiro Kurata, Maki Hirata, Shoji Watabe, Masashi Miyake, Y Susumu Takahashi and Yoshimi Yamamoto :
Expression, purification, and inhibitory activities of mouse cytotoxic T-lymphocyte antigen-2alpha.,
Protein Expression and Purification, Vol.32, No.1, 119-125, 2003.
(DOI: 10.1016/S1046-5928(03)00222-5,   PubMed: 14680948)

総説・解説:

1. Fuminori Tanihara, Maki Hirata and Takeshige Otoi :
Current status of the application of gene editing in pigs.,
The Journal of Reproduction and Development, Vol.67, No.3, 177-187, Apr. 2021.
(DOI: 10.1262/jrd.2021-025,   PubMed: 33840678)

国際会議:

1. Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
2. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Hirano Takayuki, Le Anh Quynh and Takeshige Otoi :
Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
3. Le Anh Quynh, Maki Hirata, Fuminori Tanihara, Nguyen Thi Nhien, Hirano Takayuki and Takeshige Otoi :
Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.
4. Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Le Anh Quynh, Hirano Takayuki and Takeshige Otoi :
Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation,
The 15th Transgenic Technology Meeting (TT2019), Kobe, Apr. 2019.

国内講演発表:

1. 平田 真樹 :
畜産領域におけるアニマルウェルフェアとOne health,
第23回臨床腸内微生物学会総会・学術集会, 2020年9月.
2. 谷原 史倫, 平田 真樹, Nguyen Thi Nhien, Quynh Anh Le, 平野 隆之, 音井 威重 :
エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製,
第162回日本獣医学会学術集会, 2019年9月.
3. 平田 真樹, 谷原 史倫, NGUYEN NHIEN THI, Namula Zhao, 音井 威重 :
ブタ体外受精卵におけるCrispr/Cas9システムを使用したゲノム編集の効率,
日本ゲノム編集学会 第3回大会, 2018年6月.
4. 谷原 史倫, 平田 真樹, NGUYEN NHIEN THI, 平野 隆之, 音井 威重 :
ブタ内在性レトロウイルス遺伝子を標的としたゲノム編集が胚発育能に及ぼす影響,
日本ゲノム編集学会第3回大会, 2018年6月.

その他・研究会:

1. 吉田 知加, 平田 真樹, 松岡 美樹, 大貫 燿, 岡 健太郎, 高橋 志達, 原口 雅宣, 森松 文毅 :
照明色の違いが豚の攻撃的行動と生産性に及ぼす影響,
LED総合フォーラム2021in徳島, P-19, 2021年2月.
2. 谷原 史倫, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 :
CRISPR/Cas9システムによるブタ体外受精卵のINS遺伝子への点変異導入,
第7回日本先進医工学ブタ研究会, 2019年10月.
3. 音井 威重, 平田 真樹, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 谷原 史倫 :
GEEP法を用いた遺伝子改変ブタの作製と遺伝子改変効率,
第7回日本先進医工学ブタ研究会, 2019年10月.
4. 平田 真樹, 谷原 史倫, NGUYEN THI NHIEN, LE ANH QUYNH, 平野 隆之, 音井 威重 :
ブタ体外受精卵におけるCRISPR/Cas9システムを用いた複数遺伝子の同時改変,
第7回日本先進医工学ブタ研究会, 2019年10月.
5. Le Anh Quynh, Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano and Takeshige Otoi :
Concentration of CRISPR/Cas9 components effects on genetic mosaicism of cytoplasmic microinjected porcine embryos,
第6回日本先進医工学ブタ研究会, Oct. 2018.
6. Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Le Anh Quynh, 新居 雅宏 and Takeshige Otoi :
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid,
第6回日本先進医工学ブタ研究会, Oct. 2018.
7. 平田 真樹, 谷原 史倫, 平野 隆之, NGUYEN NHIEN THI, LE ANH QUYNH, 新居 雅宏, 音井 威重 :
ブタにおける受精前後でのゲノム編集が胚盤胞の変異導入効率に及ぼす影響,
第6回日本先進医工学ブタ研究会, 2018年10月.
8. 谷原 史倫, 平田 真樹, NGUYEN NHIEN THI, LE ANH QUYNH, 平野 隆之, 竹本 龍也, 中井 美智子, 淵本 大一郎, 音井 威重 :
ゲノム編集によるTP53遺伝子改変ブタの作製と表現型の解析,
第6回日本先進医工学ブタ研究会, 2018年10月.
9. Fuminori Tanihara, Tatsuya Takemoto, Maki Hirata, N Nguyen Thi, Toshiki Kunihara, R Nishinakamura and Takeshige Otoi :
Modification of SALL1 gene via CRISPR/Cas9-mediated gene editing introduced into porcine zygotes by electroporation,
KEY Forum: The 3rd International symposium on Stem Cell Traits and Developmental Systems, Jan. 2018.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

  • ゲノム編集技術を用いた免疫不全マイクロミニブタの創出 (研究課題/領域番号: 18K12062 )
  • 気道・肺の障害・修復と再生におけるp53の役割~p53遺伝子改変ブタを用いた研究 (研究課題/領域番号: 18K08785 )
  • 研究者番号(10815583)による検索