Animal gastrointestinal tracts are populated by highly diverse and complex microbiotas. The gut microbiota influences the bioavailability of dietary components and is closely associated with physiological processes in the host. Clostridium butyricum reportedly improves growth performance and affects the gut microbiota and immune functions in post-weaning piglets. However, the effects of C. butyricum on finishing pigs remain unclear. Therefore, we herein investigated the effects of C. butyricum MIYAIRI 588 (CBM588) on the gut microbiota of finishing pigs. 16S rRNA gene sequencing was performed using fecal samples and ileal, cecal, and colonic contents collected after slaughtering. The α-diversity of the small intestinal microbiota was lower than that of the large intestinal microbiota, whereas β-diversity showed different patterns depending on sample collection sites. The administration of CBM588 did not significantly affect the α- or β-diversity of the microbiotas of fecal and intestinal content samples regardless of the collection site. However, a linear discriminant ana-lysis Effect Size revealed that the relative abundance of Lactobacillaceae at the family level, Bifidobacterium at the order level, and Lactobacillus ruminis and Bifidobacterium pseudolongum at the species level were higher in the fecal samples and cecal and colonic contents of the treatment group than in those of the control group. Therefore, the administration of CBM588 to finishing pigs affected the composition of the gut microbiota and increased the abundance of bacteria that are beneficial to the host. These results provide important insights into the effects of probiotic administration on relatively stable gut microbial ecosystems.
Misako Ezoe, Jun-Ichi Wakamatsu, Yoshihisa Takahata, Takanori Hasegawa, Fumiki Morimatsu and Takanori Nishimura : Diet-Induced Thermogenesis and Expression Levels of Thyroid Hormone Target Genes and Their Products in Rats Differ between Meat Proteins., Journal of Nutritional Science and Vitaminology, Vol.62, No.2, 93-100, 2016.
(要約)
We compared the effects of purified meat proteins on postprandial thermogenesis and on the secretion of and responsiveness to thyroid hormones (THs) in rats. Body temperatures at 2 h after feeding were significantly higher in the chicken and mutton protein groups than in the other groups, and these proteins seem to have a strong thermogenic effect. There were no significant differences in plasma TH concentrations among the groups, but levels of TH-responsive Spot 14 protein in the liver and brown adipose tissue were significantly higher in the chicken and mutton protein groups than in the other groups. Levels of malic enzyme 1 protein in the liver and brown adipose tissue were significantly higher in the chicken protein group than in the other groups except for the mutton protein group. Furthermore, levels of uncoupling protein 1 were higher in the chicken and mutton protein groups than in the other groups. The results suggest that the difference in postprandial thermogenesis of meat is strongly dependent on meat proteins; chicken and mutton proteins are strong promoters of postprandial thermogenesis, and THs may contribute to this effect. Since strong postprandial thermogenesis and high expression levels of TH target genes and their products were not observed in the amino acid group, chicken and mutton proteins or their digested peptides might contribute to these effects.
Yasutaka Nishiyama, Kumiko Kamiya, Takashi Matsumoto and Fumiki Morimatsu : The validation of the NH Immunochromato O157 test kit., Journal of AOAC International, Vol.98, No.2, 379-389, 2015.
(要約)
NH Immunochromato O157 is an immunochromatographic test for detection of Escherichia coli O157:H7 and O157:NM in food. It enables simple and rapid testing for the target organism after 18-24 h enrichment. In inclusivity and exclusivity testing, all 50 O157:H7 strains and 15 O157:NM strains tested positive, while all 33 exclusivity strains yielded negative results. Taken together, all 98 strains tested in inclusivity/exclusivity testing were identified correctly. NH Immunochromato O157 method was compared to U.S. Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook, Chapter 5.08, reference method for detection of E. coli O157:H7 in 25 g of raw ground beef. The performance of both methods was revealed to be statistically equivalent. Autoclaved and non-autoclaved sample enrichments yielded the same results, showing sterilization is not mandatory for testing with NH Immunochromato O157. The results of the robustness study were not statistically different in all conditions, suggesting that NH Immunochromato produce reliable results under various conditions. However, the users are recommended to follow the instruction when applying sample to the test strip, because a smaller sample volume may produce invalid result.
Yoshitaka Terao, Kana Takeshita, Yasutaka Nishiyama, Naoki Morishita, Takashi Matsumoto and Fumiki Morimatsu : Promising Nucleic Acid Lateral Flow Assay Plus PCR for Shiga Toxin-Producing Escherichia coli., Journal of Food Protection, Vol.78, No.8, 1560-1568, 2015.
(要約)
Shiga toxin (Stx)-producing Escherichia coli (STEC) is a frequent cause of foodborne infections, and methods for rapid and reliable detection of STEC are needed. A nucleic acid lateral flow assay (NALFA) plus PCR was evaluated for detecting STEC after enrichment. When cell suspensions of 45 STEC strains, 14 non-STEC strains, and 13 non-E. coli strains were tested with the NALFA plus PCR, all of the STEC strains yielded positive results, and all of the non-STEC and non-E. coli strains yielded negative results. The lower detection limit for the STEC strains ranged from 0.1 to 1 pg of genomic DNA (about 20 to 200 CFU) per test, and the NALFA plus PCR was able to detect Stx1- and Stx2-producing E. coli strains with similar sensitivities. The ability of the NALFA plus PCR to detect STEC in enrichment cultures of radish sprouts, tomato, raw ground beef, and beef liver inoculated with 10-fold serially diluted STEC cultures was comparable to that of a real-time PCR assay (at a level of 100 to 100,000 CFU/ml in enrichment culture). The bacterial inoculation test in raw ground beef revealed that the lower detection limit of the NALFA plus PCR was also comparable to that obtained with a real-time PCR assay that followed the U.S. Department of Agriculture guidelines. Although further evaluation is required, these results suggest that the NALFA plus PCR is a specific and sensitive method for detecting STEC in a food manufacturing plant.
(キーワード)
Animals / Cattle / DNA Primers / Food Contamination / Food Microbiology / Guidelines as Topic / Limit of Detection / Lycopersicon esculentum / Raphanus / Real-Time Polymerase Chain Reaction / Red Meat / Seedlings / Sensitivity and Specificity / Shiga-Toxigenic Escherichia coli / United States / United States Department of Agriculture
Fumihiro Arakawa, Masaki Kozono, Tomoko Ishiguro, Kousaku Yamaguchi, Yasuhiro Ihara, Yasuyuki Oishi and Fumiki Morimatsu : Survey of content of trans-fatty acids in meat, Japanese Journal of Food Chemistry and Safety, Vol.21, No.1, 1-7, 2014.
Naoki Morishita, Takashi Matsumoto, Fumiki Morimatsu and Masatake Toyoda : Detection of soybean proteins in fermented soybean products by using heating extraction., Journal of Food Science, Vol.79, No.5, 1049-1054, 2014.
(要約)
Soybean is used in processed foods worldwide. Because soybean can cause adverse reactions in some atopic patients, appropriate labeling regarding its content in processed foods is needed to better protect consumers. In the previous study, we developed a reliable sandwich Enzyme Linked Immunosorbent Assay (ELISA) method with high sensitivity and specificity for detecting soybean proteins by using antibody to Gly m Bd 30K, which was originally characterized as a vacuolar protein with a molecular mass of 34 kDa in soybean. The ELISA displayed satisfactory repeatability and reproducibility in an interlaboratory evaluation. However, it could not detect soybean protein in fermented soybean products. We therefore developed an extraction method combined with a heating process to inhibit soybean protein degradation by microbial proteolytic enzymes in fermented soybean products. This extraction method enables the sensitive detection of soybean protein in fermented soybean products such as natto and miso. It was able to detect with high-sensitivity soybean protein present at 10 μg/g levels in model processed foods. This method is suitable for quantifying soybean protein in processed foods without the degrading effects of microbial proteolytic enzymes. The present extraction method can be used sensitively to monitor labeling systems in a reliable manner and should be useful for the mandatory inspections required under Japanese regulations. The extraction and ELISA methods that we developed enable sensitive detection of soybean protein in soybean products, including fermented foods. These methods should be useful for reliable and sensitive monitoring of product labeling systems and should help to solve the problem of insensitive in soybean labeling of processed foods.
(キーワード)
Antibodies / Enzyme-Linked Immunosorbent Assay / 発酵 (fermentation) / Heating / Hot Temperature / Humans / Reproducibility of Results / Soy Foods / Soybean Proteins / Soybeans
Probiotic bacteria such as lactic acid bacteria (LAB) have recently received attention as candidates for alternative anti-microbial feed additives. We previously isolated Enterococcus faecium strain NHRD IHARA (FERM BP-11090, NHRD IHARA strain) and reported its probiotic efficacy. However, we have not determined the effect of oral administration of heat-killed cells of this strain. Here, we performed two experiments to investigate the effect of oral administration of the heat-killed NHRD IHARA strain on post-weaning piglets. In Experiment 1, there was a significant improvement in growth performance (P = 0.04) and increase in serum immunoglobulin A (IgA) production (P = 0.03) in the group fed heat-killed cells. These results were similar to previous results we obtained with live cells. We also found changes in serum and fecal IgA production that were unrelated to the patterns of microbiotal change. In Experiment 2, we detected a significant improvement in villus growth in the jejunum (P = 0.0002). In conclusion, oral administration of the heat-killed NHRD IHARA strain in post-weaning piglets had the same efficacy as administration of the live strain. The heat-killed NHRD IHARA strain can be used as feed additives to improve pig growth and health on commercial farms.
(キーワード)
Administration, Oral / Animal Feed / Animals / Dietary Supplements / Enterococcus faecium / Hot Temperature / Immunoglobulin A / Intestines / Probiotics / Swine / Weaning
Shin Sukegawa, Takeshi Miyake, Takayuki Ibi, Yoichi Takahagi, Hiroshi Murakami, Fumiki Morimatsu and Takahisa Yamada : Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle., Animal Science Journal, Vol.85, No.3, 193-197, 2014.
(要約)
Marbling in beef, measured by Beef Marbling Standard (BMS) number, is an economically important trait for beef cattle breeding and markets in Japan. We previously detected three single nucleotide polymorphisms (SNPs) associated with BMS number of Japanese Black in Oita prefecture: c.*188G>A in AKIRIN2, g.1471620G>T in EDG1 and g.3109537C>T in RPL27A. Here, we carried out single and multiple marker association analyses for the three SNPs in a different commercial Japanese Black population of 892 genotyped animals. The single marker analyses with the model including a single SNP showed significant associations of all SNPs with BMS number. The multiple marker analysis with the model including the main effects of the three SNPs and their interactions detected only significant main effects of g.1471620G>T and g.3109537C>T and a significant interaction between c.*188G>A and g.1471620G>T. These findings suggest the presence of inter-allelic interactions among genes affecting the development of beef marbling. For effective marker-assisted selection for BMS number, interactions among these markers need to be considered.
Noriki Kohyama, Naomi Soga, Satoko Ohashi, Makoto Hattori, Tadashi Yoshida, Takanori Hasegawa, Yasuyuki Oishi, Fumiki Morimatsu and Koji Takahashi : Improved Functional Properties of Potato Starch through Simultaneous Heat-moisture Treatment and Conjugation with Lysine and Fatty Acylated Saccharide by the Maillard Reaction, Journal of Applied Glycoscience, Vol.60, No.3, 147-153, 2013.
Jun-ichi Wakamatsu, Naomasa Takabayashi, Misako Ezoe, Takanori Hasegawa, Tatsuya Fujimura, Yoshihisa Takahata, Fumiki Morimatsu and Takanori Nishimura : Postprandial thermic effect of chicken involves thyroid hormones and hepatic energy metabolism in rats., Journal of Nutritional Science and Vitaminology, Vol.59, No.6, 516-525, 2013.
(要約)
We investigated the postprandial thermic effect of chicken and its mechanisms in rats. A chicken diet showed a strong thermic effect after consumption, and the removal of fat induced more rapid and stronger thermogenesis. Although thermogenesis induced by a purified chicken protein diet was also strong, the thermic reaction was not so rapid and a remarkable rise of peripheral temperatures was not observed. Defatted chicken and purified chicken protein activated the thyroid hormone system and up-regulated rate-limiting enzyme genes of glucose metabolism and the tricarboxylic acid (TCA) cycle in the liver. Moreover, chicken protein up-regulated the mRNA expression of a rate-limiting enzyme of hepatic lipid metabolism. It is possible that the mechanisms by which body temperature is raised are different between chicken protein and defatted chicken. On the other hand, it is possible that chicken fat suppressed the expression of energy metabolism-related genes that was induced by the consumption of lean chicken. As a result, a rise of postprandial body temperature might not have been induced after consumption of chicken fat. These results suggest that the consumption of lean chicken activates the thyroid hormone system and hepatic energy metabolism and consequently induces the postprandial thermic effect of chicken.
(キーワード)
Animals / Body Temperature / Chickens / Diet / エネルギー代謝 (energy metabolism) / Lipid Metabolism / Liver / Male / Postprandial Period / Rats / Rats, Wistar / Thermogenesis / Thyroid Hormones
Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Fumiki Morimatsu, Takayuki Kurazono, Toyoko Hiroi, G Balakrish Nair and Hisao Kurazono : Development of an immunochromatographic test strip for detection of cholera toxin., BioMed Research International, Vol.2013, No.679038 679038, 1-7, 2013.
(要約)
Because cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae infection, detection of CT is critical for diagnosis of the disease. In this study, we constructed an immunochromatographic test strip for detection of CT (CT-IC) with polyclonal antibodies developed against purified recombinant whole CT protein. The detection limit of the CT-IC was 10 ng/mL of purified recombinant CT, and it could detect the CT in culture supernatant of all 15 toxigenic V. cholerae isolates examined, whereas no false-positive signal was detected in all 5 nontoxigenic V. cholerae isolates examined. The specificity of the CT-IC was examined with recombinant heat-labile toxin (LT), which shares high homology with CT, and it was revealed that the minimum detection limit for LT was 100 times higher than that for CT. In addition, lt gene-positive enterotoxigenic Escherichia coli (ETEC) was examined by CT-IC. The false-positive signals were observed in 3 out of 12 ETEC isolates, but these signals were considerably faint. The CT-IC did not develop false-positive signals with all 7 V. parahaemolyticus isolates. These results showed the high specificity of CT-IC and the feasible use of it for the detection and surveillance of toxigenic V. cholerae.
Shengbin Rao, Shin Sukegawa, Yasuhiro Ihara, Fumihiro Arakawa, Hiroshi Murakami, Tatsuya Fujimura and Fumiki Morimatsu : Characterization of probiotic properties of Enterococcus faecium NHRD IHARA isolated from porcine feces., Bioscience, Biotechnology, and Biochemistry, Vol.77, No.9, 1967-1969, 2013.
(要約)
We examined in vitro the adhesion of Enterococcus faecium NHRD IHARA (NHRD IHARA) to porcine small intestinal mucin (PSIM) and inhibition of the adherence of enteropathogenic bacteria due to pre-incubation of PSIM with NHRD IHARA. NHRD IHARA exhibited an effective barrier function in porcine small intestinal mucus layer.
Taro Yonekita, Ryuji Ohtsuki, Eri Hojo, Naoki Morishita, Takashi Matsumoto, Tomoyasu Aizawa and Fumiki Morimatsu : Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli., Journal of Microbiological Methods, Vol.93, No.3, 251-256, 2013.
(要約)
The binding capacity of peptides with broad antimicrobial activity, or antimicrobial peptides (AMPs), to microbes has recently been applied to the specific detection of bacteria and viruses. We established a novel lateral flow assay (LFA) that combines AMPs labeled with colloidal gold and a target-specific antibody immobilized on a nitrocellulose membrane. α-Helical AMPs, especially cecropin P1 (CP1), magainin 2 (MG2), and ceratotoxin A (CtxA), were shown to have optimal properties as probes in LFA. We also established a multiplex LFA for the simultaneous detection and identification of three serogroups of Shiga toxin-producing Escherichia coli (STEC) using the CP1 probe with polyclonal antibodies anti-O157, anti-O26, and anti-O111. Each serogroup of E. coli could easily and rapidly be detected by multiplex LFA using CP1 and each was clearly visualized in a different position on the LFA strip. The multiplex LFA could detect all tested E. coli strains from serogroups O157 (22/22), O26 (17/17), and O111 (7/7), and the detection limit was 10(4)CFU/mL. No other serogroups of E. coli, including STEC O45, O91, O103, O121, and O145, or non-E. coli strains, reacted. The multiplex LFA could detect E. coli O157, O26, and O111 in food samples at very low levels (6.3, 2.9, and 5.6 CFU per 25 g of ground beef, respectively) after 18-h enrichment, and these results were in accordance with the results of the culture method, immunochromatography (IC) strip, and PCR. Given the broad binding capacity, AMP probes in combination with specific antibodies in the novel multiplex LFA may have the potential to detect various microbes simultaneously with identification on a single strip.
(キーワード)
Antibodies / Antimicrobial Cationic Peptides / Bacteriological Techniques / Protein Binding / Sensitivity and Specificity / Shiga-Toxigenic Escherichia coli
Yoshitaka Terao, Taro Yonekita, Naoki Morishita, Tatsuya Fujimura, Takashi Matsumoto and Fumiki Morimatsu : Potential rapid and simple lateral flow assay for Escherichia coli O111., Journal of Food Protection, Vol.76, No.5, 755-761, 2013.
(要約)
We developed and evaluated a lateral flow assay (LFA) as a simple and rapid method for direct detection of Escherichia coli O111 in food after enrichment. When cell suspensions of 8 E. coli O111 strains and 77 non-E. coli O111 strains were tested with the LFA, the former all yielded positive results and the latter all yielded negative results. The minimum detection limits for the E. coli O111 strains were 1.8 × 10(3) to 5.6 × 10(5) CFU/ml of cell suspension, and the LFA was able to detect live cultures or those killed by autoclaving at nearly the same level of sensitivity. To evaluate the ability of LFA to detect its target in food, enrichment cultures of meat samples inoculated with 10-fold serial dilutions of E. coli O111 were tested with the LFA and PCR. Even when there were very few E. coli O111 cells in the meat samples (1.6 × 10(0) to 1.6 × 10(1) CFU/25 g of food), when they were cultured in modified E. coli broth with novobiocin for 22 h at 42°C, the LFA yielded positive results that corresponded to the PCR results. Although the LFA requires further evaluation and field study, these results suggest that this assay has sufficient sensitivity and specificity. This procedure can be completed with a one-step incubation after the test strip has been inserted into the sample after 22 h of culture, whereas the standard culture method requires multiple cultures, skilled personnel, a well-equipped laboratory, and 4 or 5 days. The speed and simplicity of this LFA make it suitable for use as part of routine screening assays in the food industry.
(キーワード)
Colony Count, Microbial / Culture Media / DNA, Bacterial / Food Contamination / Food Microbiology / Polymerase Chain Reaction / Reagent Strips / Reproducibility of Results / Sensitivity and Specificity / Shiga-Toxigenic Escherichia coli / Time Factors
Tomomi Kouguchi, Takashi Ohmori, Muneshige Shimizu, Yoshihisa Takahata, Yoshiaki Maeyama, Takuya Suzuki, Fumiki Morimatsu and Soichi Tanabe : Effects of a chicken collagen hydrolysate on the circulation system in subjects with mild hypertension or high-normal blood pressure., Bioscience, Biotechnology, and Biochemistry, Vol.77, No.4, 691-696, 2013.
(要約)
We investigated the effects of a chicken collagen hydrolysate (CCH) on the circulation system in humans. A total of 58 subjects with either mild hypertension (systolic blood pressure (SBP) of 140-159 mmHg or diastolic blood pressure (DBP) 90-99 mmHg) or high-normal blood pressure (SBP 130-139 mmHg or DBP 85-89 mmHg) were assigned to two groups, one involving a placebo and the other, the test food (including CCH of 2.9 g/d). The parameters related to each subject's circulation system were monitored over the study period of 18 weeks. The Δbrachial-ankle pulse wave velocity (baPWV), an indicator of arterial stiffness and marker of vascular damage, was significantly lower in the test food group than in the placebo group during the treatment period. The blood pressure in the test food group was also significantly lower than that in the placebo group, while the serum nitrogen oxide was higher in the test food group after the treatment. These results suggest that CCH exerted modulatory effects on the human circulation system.
(キーワード)
Animals / Blood Chemical Analysis / Blood Circulation / 血圧 (blood pressure) / Body Mass Index / Body Weight / Cardiovascular System / Chickens / コラーゲン (collagen) / Female / Heart Rate / Humans / Hydrolysis / 高血圧 (hypertension) / Male / Middle Aged / Pulse Wave Analysis / Urinalysis
Taro Yonekita, Tatsuya Fujimura, Naoki Morishita, Takashi Matsumoto and Fumiki Morimatsu : Simple, rapid, and reliable detection of Escherichia coli O26 using immunochromatography., Journal of Food Protection, Vol.76, No.5, 748-754, 2013.
(要約)
Shiga toxin-producing Escherichia coli (STEC) O26 has been increasingly associated with diarrheal disease all over the world. We developed an immunochromatographic (ic) strip for the rapid detection of E. coli O26 in food samples. To determine the specificity of the IC strip, pure cultures of 67 E. coli and 22 non-E. coli strains were tested with the IC strip. The IC strip could detect all (18 of 18) E. coli O26 strains tested and did not react with strains of any other E. coli serogroup or non-E. coli strains tested (0 of 71). The minimum detection limits for E. coli O26 were 2.2 × 10(3) to 1.0 × 10(5) cfu/ml. To evaluate the ability of the IC strip to detect E. coli O26 in food, 25-g food samples (ground beef, beef liver, ground chicken, alfalfa sprout, radish sprout, spinach, natural cheese, and apple juice) were spiked with E. coli O26. The IC strip was able to detect E. coli O26 at very low levels (approximately 1 cfu/25 g of food samples) after an 18-h enrichment, and the IC strip results were in 100% agreement with the results of the culture method and pcr assay. When 115 meat samples purchased from supermarkets were tested, 5 were positive for E. coli O26 with the IC strip; these results were confirmed with a pcr assay. These results suggest that the IC strip is a useful tool for detecting E. coli O26 in food samples.
Mao Nagasawa, Tatsuro Murakami, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu and Mitsuhiro Furuse : Dietary animal proteins alter monoamine metabolism in the brain., Animal Science Journal, Vol.83, No.6, 493-498, 2012.
(要約)
Several amino acids have effects on mental function, including sedative, antidepressant-like and anxiolytic-like effects. However, the influence of integrated amino acid nutrition as protein constituents on mental function remains unclear. Therefore, the purpose of the present study was to compare the influence of chicken, pork and beef protein extracts on brain monoamine metabolism in mice. Changes in monoamine levels and their turnover rates in the brain were induced by different protein sources. In particular, chicken protein group showed the highest norepinephrine levels in the hippocampus and hypothalamus, and beef protein extract caused an activation of the serotonergic system in the hypothalamus, although there were no significant differences in amino acid compositions of these protein extracts. Therefore, it was revealed that amino acid compositions in dietary protein did not induce alteration in monoamine metabolism. However, there were differences in small molecular peptides, such as creatine, carnosine and anserine levels in animal protein extracts. In conclusion, monoamine metabolism was altered by dietary protein sources. However, it was indicated that the alteration in monoamine metabolism may be independent from amino acid compositions in dietary protein. In addition, alteration in monoamine metabolism depending on the dietary protein sources may be induced by small molecular peptides.
Takayuki Miyaji, Mikako Sato, Hirohiko Maemura, Yoshihisa Takahata and Fumiki Morimatsu : Expression profiles of carnosine synthesis-related genes in mice after ingestion of carnosine or ß-alanine., Journal of the International Society of Sports Nutrition, Vol.9, No.1, 15, 2012.
(要約)
Carnosine is a dipeptide that improves exercise performance. The carnosine synthesis mechanism through carnosine and ß-alanine ingestion remains unclear. Therefore, we investigated the tissue distribution of carnosine synthase, ATP-grasp domain-containing protein-1 (ATPGD1) mRNA, and ATPGD1 and carnosine specific dipeptidase (CN1) gene expression profiles in mice that were given carnosine or ß-alanine orally. ddY mice (7-week-old) were randomly divided into three groups (n = 6 to 8 animals per group) and were orally given 2 g/kg body weight of carnosine, ß-alanine, or water. After 15, 30, 60, 120, 180, or 360 min of treatment, the tissues (brain, blood, liver, kidneys, olfactory bulbs, hindleg muscles) were collected. The obtained tissues measured the expression of ATPGD1 and CN1 genes using quantitative PCR methods. The ATPGD1 gene was expressed in muscle and to a lesser extent in brain. The expression of ATPGD1 in the vastus lateralis muscle increased significantly at 180 min (P = 0.023) after carnosine ingestion and 60 (P = 0.023) and 180 min (P = 0.025) after ß-alanine ingestion. Moreover, the carnosine group showed a significantly increased renal expression of the CN1 gene 60 min after ingestion (P = 0.0015). The ATPGD1 gene showed high expression levels in brain and muscle. The ß-alanine or carnosine administration significantly increased ATPGD1 and CN1 expression in mice.
Mao Nagasawa, Tatsuro Murakami, Shozo Tomonaga, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu and Mitsuhiro Furuse : Impacts of acute imipramine treatment on plasma and brain amino acid metabolism in mice given graded levels of dietary chicken protein., Animal Science Journal, Vol.83, No.12, 777-787, 2012.
(要約)
Several studies have shown a relationship between depression and animal protein intake. To evaluate whether the difference of dietary chicken protein levels induces an antidepressant-like effect and potentiates acute antidepressant effects, three levels of dietary chicken protein were used as the representative animal protein with imipramine used as the antidepressant. In addition, the effects of dietary chicken protein on brain metabolism were evaluated. Open field test (OFT) and forced swimming test (FST) were conducted on the 27th and 28th days, respectively. OFT and FST were not influenced by both imipramine and dietary protein levels. However, characteristic effects of imipramine treatment on brain monoamine metabolism were observed in the cerebral cortex and hypothalamus. In addition, dietary protein significantly increased taurine and L-ornithine levels even though these amino acids were not contained in the diets. In conclusion, the metabolism of several amino acids in the plasma and brain were altered by dietary chicken protein.
Makiko Yamashita, Akira Hitaka, Himiko Fujino, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu, Tsukasa Fujiki and Yoshinori Katakura : Identification of Immunopotentiating Lactic Acid Bacteria that Induce Antibody Production by in vitro Stimulated Human Peripheral Blood Mononuclear Cells., Bioscience of Microbiota, Food and Health, Vol.31, No.1, 7-13, 2011.
(要約)
L-leucyl-L-leucine methyl ester (LLME) is known to remove lysosome-rich cells from human peripheral blood mononuclear cells (PBMCs). To evaluate the immunopotentiating ability of lactic acid bacteria (LAB), we adopted the in vitro stimulation protocol of LLME-treated PBMCs as a model assay system and monitored the level of antibody produced by stimulated PBMCs. The results indicated that several LAB strains have immunopotentiating ability against PBMCs, as evidenced by the enhanced antibody production and increased number of antigen-specific B cells. Next, we identified T cells as the direct target cells of the immunopotentiating LAB strain L32, suggesting that L32 induced antibody production by PBMCs through T-cell activation. Finally, we tested the immunopotentiating ability of ligands for Toll-like receptor 2 (TLR2), which is known to mediate the LAB signal, and observed that both L32 and one of the TLR2 ligands, LTA-BS, induced antigen-specific antibody production by in vitro stimulated PBMC. This suggests that L32 and LTA-BS can be used as an adjuvant for stimulating immune reaction in PBMCs.
Yasuo Kobayashi, Aya Itoh, Kanae Miyawaki, Satoshi Koike, Osamu Iwabuchi, Yuji Iimura, Yuri Kobashi, Tomoyuki Kawashima, Junichi Wakamatsu, Akihito Hattori, Hiroshi Murakami, Fumiki Morimatsu, Takashi Nakaebisu and Takeshi Hishinuma : Effect of liquid whey feeding on fecal microbiota of mature and growing pigs., Animal Science Journal, Vol.82, No.4, 607-615, 2011.
(要約)
The effect of liquid whey feeding on fecal bacteria and their metabolites was assessed in five pregnant sows and 66 growing pigs. Sows were fed a control diet for 4 weeks (control period) followed by the same diet but with whey feeding (5 L/day/pig) for 4 weeks (whey period). One group of growing pigs was given 267 L of whey per pig (whey group), while the other group was not (control group). In both cases, liquid whey was given separately from control diet. Sows in the whey period had feces showing lower pH, lower ammonia concentration, and larger population sizes of total bacteria, lactobacilli and bifidobacteria. The bacterial gene library analysis indicated that Mitsuokella and Megasphaera were more frequently detected, while Clostridium disporicum were detected less frequently in the whey period. Feces from whey-fed growing pigs showed lower pH than that from control pigs in the early stage of growing. Also, larger populations of total bacteria, lactobacilli and bifidobacteria were recorded in the whey group. From the bacterial gene library analysis, the detection frequency of Lactobacillus reuteri tended to be higher in the whey group. These results indicate that whey feeding influences the hindgut microbiota of pigs, possibly leading to a fermentation shift that is favorable for animal health.
Yamada Takahisa, Sasaki Seiki, Sukegawa Shin, Takahagi Youichi, Morita Mitsuo, Murakami Hiroshi, Fumiki Morimatsu and Fujita Tatsuo : Possible Association of Single Nucleotide Polymorphism in Titin Gene with Growth-Related Trait in Japanese Black Beef Cattle, Journal of Animal and Veterinary Advances, Vol.10, No.19, 2603-2606, 2011.
Keishi Kadooka, Asuka Imahayashi, Ayaka Koiso, Makiko Yamashita, Kiichiro Teruya, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu and Yoshinori Katakura : Establishment of a novel method of screening anti-allergic lactic acid bacteria., Bioscience, Biotechnology, and Biochemistry, Vol.75, No.5, 1016-1018, 2011.
(要約)
In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.
Mari Watanabe-Kamiyama, Muneshige Shimizu, Shin Kamiyama, Yasuki Taguchi, Hideyuki Sone, Fumiki Morimatsu, Hitoshi Shirakawa, Yuji Furukawa and Michio Komai : Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats., Journal of Agricultural and Food Chemistry, Vol.58, No.2, 835-841, 2010.
(要約)
Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [(14)C]proline (Pro group) or glycyl-[(14)C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolyl-hydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.
K Kawatsu, M Taguchi, T Yonekita, T Matsumoto, Fumiki Morimatsu and Y Kumeda : Simple and rapid detection of Campylobacter spp. in naturally contaminated chicken-meat samples by combination of a two-step enrichment method with an immunochromatographic assay., International Journal of Food Microbiology, Vol.142, No.1-2, 256-259, 2010.
(要約)
A simple and rapid method to detect Campylobacter spp. in chicken-meat samples was established. This method consisted of a combination of a two-step enrichment method with a commercially available immunochromatographic assay, named NH Immunochromato Campylobacter (NH IC Campy, Nippon Meat Packers, Ibaraki, Japan), which is able to detect Campylobacter antigen in an enrichment culture within 15 min. The enrichment method did not require much blood or a particular system of generating a microaerobic atmosphere, in contrast to the standard method of enriching Campylobacter spp. in chicken-meat samples. The sensitivity of a combination of the two-step enrichment method with NH IC Campy for detection of non- and freeze-stressed Campylobacter spp. in spiked chicken meat was determined using bacterial cells of Campylobacter jejuni and Campylobacter coli. The detection sensitivities for non-stressed C. jejuni and C. coli were found to range from 5.5 to 1.3x10(1) CFU per 25 g of chicken meat, and those for freeze-stressed C. jejuni and C. coli were found to range from 9.2x10(1) to 1.5x10(2) CFU per 25 g of chicken meat. When a total of 68 chicken-meat samples were tested, the combination method determined that 61 samples were positive for Campylobacter spp. This method was more sensitive than a bacterial culture test, which consists of standard enrichment culturing and plating onto selective agars. Because the combination could be conducted in approximately 48 h, from the beginning of the enrichment culture to final determination, it was more rapid than the bacterial culture test, which requires four to five days. Moreover, the combination was simple to perform. These results suggest that combining the two-step enrichment method with NH IC Campy is useful as a simple and rapid alternative to the conventional bacterial culture test for detecting Campylobacter spp. in naturally contaminated chicken meat samples.
F Nagatomo, N Gu, H Fujino, T Okiura, Fumiki Morimatsu, I Takeda and A Ishihara : Effects of exposure to hyperbaric oxygen on oxidative stress in rats with type II collagen-induced arthritis., Clinical and Experimental Medicine, Vol.10, No.1, 7-13, 2010.
(要約)
Arthritis was induced in 9-week-old female Dark Agouti rats by injecting type II collagen. Serum levels of the derivatives of reactive oxygen metabolites (dROMs), which are oxidative stress markers, and C-reactive protein (CRP) in arthritic rats that were exposed to a pressure of 1.25 atmospheres absolute and an oxygen concentration of 36% for 3 weeks (arthritis + HBO group) were compared to those of control rats (control group) and arthritic rats that were not exposed to hyperbaric oxygen (arthritis group). The body weights of the arthritis and arthritis + HBO groups were lower than that of the control group, whereas no difference in the body weight was observed between the arthritis and arthritis + HBO groups. The serum levels of dROMs and CRP in the arthritis group were higher than those in the control and arthritis + HBO groups. No difference in the serum level of CRP was observed between the control and arthritis + HBO groups. These results indicate that the conditions of hyperbaric oxygen exposure used in this study are effective for reducing the levels of reactive oxygen species, which are overproduced during arthritis.
(キーワード)
Animals / 関節炎 (arthritis) / Atmospheric Pressure / Body Weight / C-Reactive Protein / Collagen Type II / Female / 酸化ストレス (oxidative stress) / Oxygen / Rats / 活性酸素種 (reactive oxygen species)
Y. Terao, T. Yonekita, K. Kamiya, N. Morishita, T. Fujimura, T. Matsumoto and Fumiki Morimatsu : Development and Evaluation of Immunochromatographic Assay for Simple Rapid Detection of Escherichia Coli O157, O26, O111 and Vero Toxin 1/2 in Food Products, International Congress of Meat Science and Technology Proceeding, 226, 2010.
38.
Youzuo Zhang, Tomomi Kouguchi, Muneshige Shimizu, Takashi Ohmori, Yoshihisa Takahata and Fumiki Morimatsu : Chicken collagen hydrolysate protects rats from hypertension and cardiovascular damage., Journal of Medicinal Food, Vol.13, No.2, 399-405, 2010.
(要約)
We previously reported that chicken collagen hydrolysate (CCH) has strong angiotensin I converting enzyme (ACE) inhibitory activity and antihypertensive effects on spontaneously hypertensive rats. Here, we investigated the chronic therapy effects of CCH on blood pressure and vascular relaxation in a cardiovascular damage model of Wistar-Kyoto rats induced by N-nitro-l-arginine methyl ester (L-NAME). Following co-treatment with CCH for 4 weeks, the increment of systolic blood pressure was suppressed significantly. At 8 weeks, the vasorelaxation of thoracic aorta increased significantly, and cardiovascular damage was ameliorated. The concentration of soluble intercellular adhesion molecule-1 (ICAM-1) in blood was reduced significantly by long-term administration of CCH, whereas the nitric oxide concentration was increased significantly at 1 hour post-treatment. The results suggest that beneficial effects of CCH result from antihypertensive function, but also from inhibition of cardiovascular damage to the endothelial cells via its ACE inhibitory activity and regulation of nitric oxide and ICAM-1, which suggests that CCH may be useful as a medicinal food for patients with cardiovascular disease.
Youzuo Zhang, Tomomi Kouguchi, Kazuo Shimizu, Mikako Sato, Yoshihisa Takahata and Fumiki Morimatsu : Chicken collagen hydrolysate reduces proinflammatory cytokine production in C57BL/6.KOR-ApoEshl mice., Journal of Nutritional Science and Vitaminology, Vol.56, No.3, 208-210, 2010.
(要約)
Effects of chicken collagen hydrolysate (CCH) on atherogenesis in apolipoprotein E-deficient C57BL/6.KOR-ApoE(shl) mice were investigated in this paper. The mice were fed on either a normal CE-2 diet (control group) or a diet containing 10% CCH (CCH group) for 12 wk. Compared with that of the control, the amount of total plasma cholesterol, total hepatic cholesterol and hepatic triglycerides in the CCH group was reduced by 14.4, 24.7 and 42.8%, respectively. Histological analysis results showed that the abundance of diffuse hepatic lipid droplets and fat vacuoles was decreased in the CCH group. Meanwhile, the concentration of proinflammatory cytokines in the CCH group plasma, including interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1), and tumor necrosis factor-alpha (TNF-alpha), was downregulated by 43.4, 17.9 and 24.1%, respectively. The present results suggested that CCH treatment might help prevent atherosclerosis through not only its lipid-lowering effects but also inhibiting expression of inflammatory cytokines.
Kazuo Shimizu, Mikako Sato, Youzuo Zhang, Tomomi Kouguchi, Yoshihisa Takahata, Fumiki Morimatsu and Makoto Shimizu : The bioavailable octapeptide Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro stimulates nitric oxide synthesis in vascular endothelial cells., Journal of Agricultural and Food Chemistry, Vol.58, No.11, 6960-6965, 2010.
(要約)
Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro (GAXGLXGP, X: Hyp), an octapeptide contained in chicken collagen hydrolysate, inhibits angiotensin I-converting enzyme activity in vitro. Intestinal Caco-2 and bovine aortic endothelial cells (BAECs) were used to investigate whether the transported GAXGLXGP improves vascular function. When GAXGLXGP was added to the apical side of Caco-2 monolayers, the intact form of GAXGLXGP was released to the basolateral side without incorporation into the cells. This transport was energy-independent but was associated with tight junction permeability. GAXGLXGP was then added to BAECs, and endothelial nitric oxide (NO) synthase (eNOS) activation was examined. GAXGLXGP at a concentration of 10 microM stimulated production of NO during a 1 h incubation. This event involved phosphorylation of eNOS at Ser(1179) without a change in the total eNOS protein level. These findings indicate that GAXGLXGP absorbed intact through the intestinal epithelium has direct effects on eNOS activity in vascular endothelial cells, leading to NO synthesis, thereby suggesting the potential for improvement in vascular function.
Kazuo Shimizu, Mikako Sato, Youzuo Zhang, Tomomi Kouguchi, Yoshihisa Takahata, Fumiki Morimatsu and Makoto Shimizu : Molecular size of collagen peptide reverses the permeability of Caco-2 cells., Bioscience, Biotechnology, and Biochemistry, Vol.74, No.5, 1123-1125, 2010.
(要約)
Intestinal permeability to size-classified fish collagen peptides (FCPs) was examined using Caco-2 monolayers. Minimum-sized FCPs were most efficiently transported across the Caco-2 monolayers. Permeability depended on peptide size. It was independent of the H(+)-coupled peptide transport system but was associated with tight junction permeability, suggesting that the paracellular pathway is responsible for transepithelial transport of collagen peptides.
Yoichi Takahagi, Shinji Toki, Yasutaka Nishiyama, Fumiki Morimatsu and Hiroshi Murakami : Differential effects of porcine circovirus type 2 (PCV2) vaccination on PCV2 genotypes at Japanese pig farms., The Journal of Veterinary Medical Science, Vol.72, No.1, 35-41, 2010.
(要約)
Infection of pigs with porcine circovirus type 2 (PCV2) causes a variety of disorders collectively referred to as porcine circovirus associated diseases (PCVADs). PCV2 isolates can be classified into two major types: PCV2a and PCV2b. In the present study, effects of vaccination on antibody titers in sera, PCV2 viremia, and shedding of PCV2 in feces were studied on Japanese commercial pig farms where vaccination of piglets against PCV2 was performed using commercially available vaccines. The effectiveness of vaccination against various PCV2 genotypes was also assessed. Among the 16 farms studied, 10 and 6 had been infected with PCV2a and PCV2b, respectively. PCV2a was further subdivided into PCV2a-1 and PCV2a-2. PCV2a-1 and PCV2a-2 prevailed on 6 and 4 farms, respectively, among the 10 farms infected with PCV2a. The PCV2 vaccines were effective in reducing PCV2 infection on commercial pig farms. Mean mortality rates were significantly decreased over 8 months after the start of the PCV2 vaccination program as compared to those before the start of the PCV2 vaccination program on farms infected with PCV2a-2 (20.8% vs. 12.1%) and PCV2b (26.5% vs. 13.7%). On the farms with PCV2a-1 infected pigs, there was no significant difference in the mean mortality rate before versus after the start of the vaccination program (14.7% vs. 14.1%). Mortality rate reduction with the PCV2 vaccination might depend on the genetic types of PCV2.
Shin Sukegawa, Takeshi Miyake, Yoichi Takahagi, Hiroshi Murakami, Fumiki Morimatsu, Takahisa Yamada and Yoshiyuki Sasaki : Replicated association of the single nucleotide polymorphism in EDG1 with marbling in three general populations of Japanese Black beef cattle., BMC Research Notes, Vol.3, 66, 2010.
(要約)
Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucleotide polymorphism (SNP), namely, c.-312A>G, in the endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene was associated with the BMS level in the Japanese Black beef cattle population of Oita prefecture, with the G allele being associated with a high level of the BMS. Thus, the c.-312A>G SNP seems to be a candidate marker for marker-assisted selection. In this study, we investigated whether this association could be replicated in 3 other independent Japanese Black cattle populations and analyzed the effect of the SNP genotypes on the carcass traits other than the BMS. Statistically significant differences in the BMS level were detected among the genotypes of the c.-312A>G SNP in the Japanese black beef cattle populations of Miyazaki (P = 0.0377) and Nagasaki (P = 0.0012) prefectures, and marginal difference was detected in the Kagoshima prefecture population (P = 0.0786). The G allele in the SNP was associated with an increase in the BMS level.The G allele also seemed to have a favorable influence, if any, on the carcass weight, rib eye area and rib thickness of the cattle populations. These findings suggest that the association of the c.-312A>G SNP with the BMS level in the Japanese Black beef cattle population was replicated in other beef cattle populations, and revealed favorable effects of the G allele on the beef productivity in the general Japanese Black beef cattle population. Thus, we concluded that the c.-312A>G SNP is useful for effective marker-assisted selection to increase the BMS level in Japanese Black beef cattle.
Muneshige Shimizu, Naoko Sawashita, Fumiki Morimatsu, Jun Ichikawa, Yasuki Taguchi, Yoshinobu Ijiri and Junichiro Yamamoto : Antithrombotic papain-hydrolyzed peptides isolated from pork meat., Thrombosis Research, Vol.123, No.5, 753-757, 2009.
(要約)
Atherothrombotic disease such as coronary artery disease and stroke is one of major causes of death. Platelets play important role in these arterial diseases. Suppression of platelet activity by materials may decrease incidence of the disease. The present study aimed to examine the peptides from pork meat with antithrombotic activity. Peptide fraction or hydrolyzate of defatted pork meat by papain was used as the starting material. Antithrombotic activity of the fraction was assessed by a share-induced platelet function test in vitro (haemostatometry) using non-anticoagulated rat blood, followed by a helium-neon laser-induced mouse carotid artery thrombosis test in vivo. The starting peptide fraction with mean molecular weight 2500 showed antithrombotic activity in vivo after oral administration to mice at 210 mg/kg body weight. The fraction with mean molecular weight 2517 further purified by cation exchange chromatography showed antithrombotic activity after oral administration at 70 mg/kg body weight. Antithrombotic activity of the purified peptide fraction was equivalent to that of aspirin at 50 mg/kg body weight. It is possible this pork peptide could be beneficial to prevent atherothrombosis.
Takahisa Yamada, Seiki Sasaki, Shin Sukegawa, Takeshi Miyake, Tatsuo Fujita, Hiroyuki Kose, Mitsuo Morita, Youichi Takahagi, Hiroshi Murakami, Fumiki Morimatsu and Yoshiyuki Sasaki : Association of a single nucleotide polymorphism in ribosomal protein L27a gene with marbling in Japanese Black beef cattle., Animal Science Journal, Vol.80, No.6, 631-635, 2009.
(要約)
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The c2-11#2 expressed sequence tag (EST) has been previously shown to possess expression difference in musculus longissimus muscle between low-marbled and high-marbled steer groups, and to be located within genomic region of a quantitative trait locus for marbling. Thus, the ribosomal protein L27a (RPL27A) gene containing the c2-11#2 EST sequence was considered as a positional candidate for the gene responsible for marbling. In the present study, a single nucleotide polymorphism (SNP) in the promoter region of the RPL27A, referred to as g.3109537C>T, was detected between the 2 steer groups. The SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. The effect of genotypes of the SNP on the predicted breeding value for subcutaneous fat thickness was not statistically significant. These findings suggest that the RPL27A SNP may be useful for effective marker-assisted selection to increase the levels of marbling in Japanese Black beef cattle.
(キーワード)
Animals / Cattle / Fats / Male / Muscle, Skeletal / Polymorphism, Single Nucleotide / Ribosomal Proteins
Takahisa Yamada, Seiki Sasaki, Shin Sukegawa, Takeshi Miyake, Tatsuo Fujita, Hiroyuki Kose, Mitsuo Morita, Youichi Takahagi, Hiroshi Murakami, Fumiki Morimatsu and Yoshiyuki Sasaki : Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle., Animal Science Journal, Vol.80, No.4, 486-489, 2009.
(要約)
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A>G in the 5' untranslated region (UTR) and c.*446G>A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G>A and g.1471620G>T, in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G>A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G>T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G>T SNP might have an impact on EDG1 expression and also marbling.
(キーワード)
5' Flanking Region / Animals / Cattle / Male / Meat / Polymorphism, Single Nucleotide
Takanori Hasegawa, Kozue Hirakawa, Takashi Matsumoto, Shinji Toki, Yoshiaki Maeyama and Fumiki Morimatsu : Efficacy of Lactobacillus plantarum strain HSK201 in relief from Japanese cedar pollinosis., Bioscience, Biotechnology, and Biochemistry, Vol.73, No.12, 2626-2631, 2009.
(要約)
To determine the effects of Lactobacillus plantarum strain HSK201 on Japanese cedar pollinosis, a single-blind, placebo-controlled study was conducted in 2008. The HSK201 group was administered fermented milk prepared with HSK201 for 8 weeks, and the placebo group was administered non-fermented milk adjusted to the same acidity and taste. We found HSK201 strain intake to suppress both helper T cell type 1/2 ratio reduction and serum Japanese cedar pollen-specific IgE elevation at the peak of pollen dispersion. In addition, the nasal and ocular symptom scores in the HSK201 group were also lower than those in the placebo group during the early phase of the pollen season. Although this was a preliminary study with 19 employees of our own company serving as subjects, the results suggest that ingestion of the HSK201 strain alleviates pollinosis symptoms during the period when pollen exposure is low and the symptoms are mild.
S. Tomonaga, M. Sato, Y. Takahata, Fumiki Morimatsu and M. Furuse : Oral Administration of chicken Breast Extract Activates Serotonin Metabolism in the Hippocampus of Rats, Journal of Animal and Veterinary Advances, Vol.8, No.11, 2776-2279, 2009.
Takaaki Mishima, Takashi Yamada, Makoto Sakamoto, Minako Sugiyama, Satoshi Matsunaga, Hirohiko Maemura, Muneshige Shimizu, Yoshihisa Takahata, Fumiki Morimatsu and Masanobu Wada : Chicken breast attenuates high-intensity-exercise-induced decrease in rat sarcoplasmic reticulum Ca2+ handling., International Journal of Sport Nutrition and Exercise Metabolism, Vol.18, No.4, 399-411, 2008.
(要約)
This study was conducted to determine whether dietary chicken-breast extract (CBEX), a rich source of histidine-containing dipeptides, could modify exercise-induced changes in sarcoplasmic reticulum (SR) function. After 5 weeks of dietary CBEX, SR Ca2+-handling ability was examined in the vastus lateralis muscles of rats subjected to high-intensity running for 2.5 min. Dietary CBEX caused an approximately 15% and 45% increase (p<.01) in muscle carnosine and anserine concentrations, respectively. In resting muscles, depressions in SR Ca2+-ATPase activity were evoked by dietary CBEX without concomitant changes in SR Ca2+ uptake and release rates. The data confirm that high-intensity exercise depresses SR Ca2+ handling. In spite of the same run time, SR Ca2+ handling was reduced to a lesser degree in muscles of CBEX-containing-chow-fed rats than in standard-chow-fed rats (p<.05). These results suggest that dietary CBEX might attenuate deteriorations in SR Ca2+-handling ability that occur with high-intensity exercise.
Shinji Toki, Shinji Kagaya, Miwa Shinohara, Hiroshi Wakiguchi, Takashi Matsumoto, Yoshihisa Takahata, Fumiki Morimatsu, Hirohisa Saito and Kenji Matsumoto : Lactobacillus rhamnosus GG and Lactobacillus casei suppress Escherichia coli-induced chemokine expression in intestinal epithelial cells., International Archives of Allergy and Immunology, Vol.148, No.1, 45-58, 2008.
(要約)
Recently, some strains of lactic acid bacteria (LAB) have been reported to prevent the development of atopic dermatitis and to improve allergic symptoms, especially in young children. However, the mechanisms involved in these effects are not fully understood. Intestinal microbiota play critical roles in the development of host immune development and are recognized and regulated by the host through intestinal epithelial cells (IECs). We thus hypothesized that LAB influence the host immune system through the activation of IECs. To begin testing this hypothesis, chemokine expression in IECs exposed to intestinal bacteria was investigated. Caco-2 cell monolayers were stimulated with different concentrations of various live or heat-killed intestinal bacteria or bacterial components for up to 3 h. Changes in the gene expressions of various chemokines were measured using quantitative real-time PCR. The expressions of CCL20, CXCL8, CXCL10 and CX3CL1 were strongly induced by nonpathogenic Escherichia coli in a dose-dependent manner and were partially induced by some commensal LAB. In contrast, Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei did not induce these chemokine expressions. In addition, LGG significantly suppressed the expressions of CCL20 and CXCL10 induced by E. coli, peptidoglycan or flagellin when cultured simultaneously. LGG and L. casei markedly suppressed E. coli-induced chemokine expression, presumably through the suppression of the Toll-like receptor-mediated signal transduction pathway, at least in part. The clinical importance of this suppressive effect and the mechanisms involved require further investigation; however, such effects can be used as a marker to identify clinically useful LAB.
Hang Guo, Ai Ekusa, Koji Iwai, Masami Yonekura, Yoshihisa Takahata and Fumiki Morimatsu : Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo., Journal of Nutritional Science and Vitaminology, Vol.54, No.3, 191-195, 2008.
(要約)
Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
Yoichi Takahagi, Yasutaka Nishiyama, Shinji Toki, Taro Yonekita, Fumiki Morimatsu and Hiroshi Murakami : Genotypic change of porcine circovirus type 2 on Japanese pig farms as revealed by restriction fragment length polymorphism analysis., The Journal of Veterinary Medical Science, Vol.70, No.6, 603-606, 2008.
(要約)
Porcine circovirus type 2 (PCV2) has been recognized as the causal agent of postweaning multisystemic wasting syndrome and can be divided into two major genotypic groups. We developed a method of restriction fragment length polymorphism (RFLP) analysis of PCV2 open reading frame 2 for easy discrimination between the two major groups. Genotyping of PCV2 isolates from 10 Japanese commercial pig farms was performed, and the analysis revealed that both PCV2 groups and at least five RFLP types of PCV2 are prevalent in Japan. On two farms, the genotypes of the PCV2 isolates in the spring of 2007 were different from those in the autumn of 2006. One genotype may have become dominant within only six months on these farms.
(キーワード)
Animals / Base Sequence / Circoviridae Infections / Circovirus / Cluster Analysis / DNA Primers / Genotype / 日本 (Japan) / Molecular Sequence Data / Phylogeny / Polymorphism, Restriction Fragment Length / Sequence Analysis, DNA / Sus scrofa / Swine Diseases
Tatsuya Fujimura, Takashi Matsumoto, Soichi Tanabe and Fumiki Morimatsu : Specific discrimination of chicken DNA from other poultry DNA in processed foods using the polymerase chain reaction., Bioscience, Biotechnology, and Biochemistry, Vol.72, No.3, 909-913, 2008.
(要約)
In the present study, specific discrimination of chicken DNA contamination in processed foods using the polymerase chain reaction was investigated. The primer pair was designed to amplify a 102-bp fragment of the chicken mitochondrial 16S ribosomal RNA gene. While the DNA from chicken meat was amplified, the DNA from other poultry meat, mammalian meat, fish, shellfish, and cereals was not amplified. The primer amplified DNA fragments derived from model processed and nonprocessed food samples containing 0.001, 0.01, 0.1, 1, 10, and 100% chicken.
Yurika Mizobe, Daichi Oikawa, Shoichiro Tsuyama, Yoriko Akimoto, Kousuke Hamasu, Eiichiro Onitsuka, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu and Mitsuhiro Furuse : mRNA expression of lysyl oxidase and matrix metalloproteinase-12 in mouse skin., Bioscience, Biotechnology, and Biochemistry, Vol.72, No.11, 3067-3070, 2008.
(要約)
Elastic fibers in the dermis play an important role in skin elasticity. The desmosine crosslinking structure constructed of lysyl oxidase (LOX) in elastic fibers contributes to elasticity, while elastic fibers are primarily degraded by one of the matrix metalloproteinases (MMPs), MMP-12. We investigated the gender differences and diurnal variation of these enzymes. Gender-based differences in LOX mRNA expression were detected, and were significantly lower in females. In contrast, higher MMP-12 mRNA expression was observed in the light period, suggesting that elastic fibers might be degraded in the light rather than the dark period.
Depression is a pathological state of mood and is considered as one of the major causes of disabilities. Thus, the prevention of depression and care for individuals with depression is important. In the present study, we examined whether a single oral dose of CBEX (chicken breast extract), or carnosine (one of the major components of CBEX) affects immobility time, an index of depressive-like behavior, in the forced swimming test in male Wistar rats. CBEX tended to (P=0.09) and carnosine significantly (P<0.05) decreased immobility time in the forced swimming test. In the hippocampus, both CBEX and carnosine significantly decreased 3-methoxy-4-hydroxyphenylglycol, a major metabolite of norepinephrine, indicating that CBEX and carnosine could reduce NE activity in the hippocampus in the forced swimming test. CBEX and carnosine did not affect total locomotive distance or rearing in the open field test, suggesting that the reductions of immobility time by both treatments in the forced swimming test were not merely due to the stimulation of general motor activity. Taken together, these results suggest that CBEX has an antidepressant-like effect, which may be due, in part, to the effect of carnosine.
Ai Saiga, Koji Iwai, Toru Hayakawa, Yoshihisa Takahata, Shiniich Kitamura, Toshihide Nishimura and Fumiki Morimatsu : Angiotensin I-converting enzyme-inhibitory peptides obtained from chicken collagen hydrolysate., Journal of Agricultural and Food Chemistry, Vol.56, No.20, 9586-9591, 2008.
(要約)
In this study, collagen extracted from chicken legs (which are the yellow keratin parts containing a nail) was hydrolyzed with various enzymes, and the angiotensin I-converting enzyme (ACE)-inhibitory activity of each hydrolysate was determined. The hydrolysate by treatment with an Aspergillus species-derived enzyme had the highest activity (IC 50 = 260 microg/mL). The fraction of this hydrolysate obtained by ultrafiltration with a molecular-weight cutoff of 3000 Da (low fraction) had a stronger activity (IC 50 = 130 microg/mL) than the fractionated one. This fraction was further fractionated by HPLC, and the peptides in the fraction with high ACE-inhibitory activity were identified. The amino acid sequences of the four peptides were identified using a protein sequencer. These peptides were synthesized to confirm their ACE-inhibitory activities; this showed that peptides with a Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro sequence had the highest activity (IC 50 = 29 microM). When the low fraction was administered to spontaneous hypertensive rats, a decrease in their blood pressure was observed after 2 h of administration, and a significant decrease in blood pressure (-50 mmHg) was observed after 6 h. Moreover, long-term administration studies indicated that the low fraction showed a significant suppression of increased blood pressure.
Tomonori Okiura, Fumiko Nagatomo, Ning Gu, Yasuki Taguchi, Fumiki Morimatsu and Akihiko Ishihara : Bone density of the femur and fiber cross-sectional area and oxidative enzyme activity of the tibialis anterior muscle in type II collagen-induced arthritic mice., The Journal of Physiological Sciences, Vol.58, No.4, 221-227, 2008.
(要約)
Femur bone densities and tibialis anterior muscle properties of type II collagen-induced arthritic mice were determined. Furthermore, voluntary running activities of arthritic mice were compared with those of controls. Arthritis was induced by an intradermal injection of type II collagen in the adjuvant. Body and muscle weights were lower in arthritic mice than in controls. Cortical and trabecular bone densities and muscle fiber cross-sectional areas were decreased by arthritis. After classifying the arthritic severity into slight, intermediate, and severe levels based on the degree of knuckle swelling, cortical and trabecular bone densities, fiber cross-sectional areas, and fiber succinate dehydrogenase activities were lowest when arthritis was most severe. Furthermore, arthritic mice, especially those with intermediate and severe levels, showed lower voluntary running activities. These findings indicate that lower bone density and muscle atrophy of type II collagen-induced arthritic mice are related to arthritic severity and decreased motor activity.
(キーワード)
Animals / Arthritis, Experimental / Body Weight / Bone Density / Collagen Type II / Femur / Male / Mice / Mice, Inbred DBA / Motor Activity / Muscle Fibers, Skeletal / Muscle, Skeletal / Muscular Atrophy / Organ Size / Running / Severity of Illness Index / Succinate Dehydrogenase
Takahisa Yamada, Seiki Sasaki, Shin Sukegawa, Youji Muramatsu, Youichi Takahagi, Sachiyo Yoshioka, Tatsuo Fujita, Mitsuo Morita, Hiroshi Murakami, Fumiki Morimatsu and Yoshiyuki Sasaki : Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes., Research Communications in Molecular Pathology and Pharmacology, Vol.120-121, No.1-6, 105-114, 2008.
(要約)
We have recently showed that the pyruvate dehydrogenase (lipoamide) beta (PDHB) gene involved in fatty acid oxidation, the sorbin and SH3 domain containing 1 (SORBS1) gene involved in insulin-stimulated glucose uptake, and the endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene involved in blood vessel formation possess expression differences in musculus longissimus muscle between low-marbled and high-marbled steer groups. In the present study, we detected single nucleotide polymorphisms (SNPs) in the promoter regions of the 3 genes between the 2 steer groups. A SNP in the EDG1 exhibited significantly different allelic distribution between animals with extremely high predicted breeding value for marbling and with extremely low one. The EDG1 SNP may be related to changes in gene expression and/or marbling.
(キーワード)
Adipose Tissue / Alleles / Animals / Body Fat Distribution / Breeding / Cattle / Male / Microfilament Proteins / Muscle, Skeletal / Polymorphism, Single Nucleotide / Promoter Regions, Genetic / Pyruvate Dehydrogenase (Lipoamide) / Receptors, Lysosphingolipid
(文献検索サイトへのリンク)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21469508
M Sato, N Karasawa, M Shimizu, Fumiki Morimatsu and R Yamada : Safety evaluation of chicken breast extract containing carnosine and anserine., Food and Chemical Toxicology, Vol.46, No.2, 480-489, 2007.
(要約)
Chicken breast extract (CBEX) is obtained via hot water extraction of chicken breast and contains among its primary constituents carnosine and anserine, which are histidine-containing dipeptides present in the muscle tissues of most vertebrate species. Dietary intake of CBEX has been previously shown to buffer hydrogen ions formed during high-intensity exercise in human skeletal muscle cells, thereby inhibiting a decrease in muscle cell pH and subsequent muscle fatigue. The objective of this paper is to report the results of safety studies completed on CBEX. CBEX was determined to have an oral LD(50) value of more than 6000 mg/kg body weight in rats. Gavage doses of 500 or 2000 mg CBEX/kg body weight/day administered to rats for 90 days produced no toxicologically significant, dose-related, differences between control and treated animals with respect to body weight gain, food consumption, behavioral effects, hematological and clinical chemistry parameters, absolute and relative organ weights, or gross and microscopic findings. In the presence or absence of metabolic activation, CBEX exerted no mutagenic activity in the Ames assay conducted in various strains of Salmonella typhimurium and Escherichia coli. The results of these studies support the safety of CBEX as a potential dietary source of carnosine and anserine.
Shozo Tomonaga, Toru Hayakawa, Haruka Yamane, Hirohiko Maemura, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu and Mitsuhiro Furuse : Oral administration of chicken breast extract increases brain carnosine and anserine concentrations in rats., Nutritional Neuroscience, Vol.10, No.3-4, 181-186, 2007.
(要約)
Carnosine (beta-alanyl-L-histidine) and its derivative anserine (beta-alanyl-1-methyl-L-histidine) are antioxidants and putative neurotransmitters in the brain. These dipeptides are rich in the commercially available supplement chicken breast extract (CBEX). To clarify the effects of CBEX on the brain, we examined whether single oral administration of CBEX (20 ml/kg) affects brain dipeptide and free amino acid concentrations in male Wistar rats. CBEX significantly and time-dependently increased carnosine and anserine levels in the plasma (at 120 min after injection, increase rates were 2976 and 4142%, respectively), hippocampus (64 and 78%), and hypothalamus (188 and 120%), but not in cerebral cortex. Significant and time-dependent increases in citrulline in the hippocampus (49%) and hypothalamus (41%) demonstrated generation of nitric oxide due to the increased carnosine and/or anserine levels in these brain regions. These findings suggest that CBEX modifies brain functions by increasing levels of these dipeptides.
Shin Sukegawa, Takahisa Yamada, Seiki Sasaki, Youji Muramatsu, Youichi Takahagi, Sachiyo Yoshioka, Tatsuo Fujita, Mitsuo Morita, Hiroshi Murakami, Fumiki Morimatsu and Yoshiyuki Sasaki : Polymorphisms for 7 expressed sequence tags showing marbling-associated expression changes., Research Communications in Molecular Pathology and Pharmacology, Vol.120-121, No.1-6, 33-42, 2007.
(要約)
We have recently showed that the 7 expressed sequence tags (ESTs), c2-11#2, c3-3, c20-29, c22-3, c26-18#1, c26-42, and g5-10, possess expression differences in musculus longissimus muscle between low-marbled and high-marbled steer groups. In the present study, we detected single nucleotide polymorphisms (SNPs) in the 5' flanking regions of the 7 EST sequences between the 2 steer groups. A SNP for the c2-11#2 EST exhibited significantly different allelic distribution between animals with extremely high predicted breeding value for marbling and with extremely low one. The SNP in the ribosomal protein L27a (RPL27A) gene containing the c2-11#2 EST sequence may be related to changes in gene expression and/or marbling.
(キーワード)
Adipose Tissue / Alleles / Animals / Body Fat Distribution / Breeding / Cattle / Expressed Sequence Tags / 遺伝子発現 (gene expression) / Male / Muscle, Skeletal / Polymorphism, Genetic / Polymorphism, Single Nucleotide
(文献検索サイトへのリンク)
● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21469502
A Matsumoto, T Okiura, Fumiki Morimatsu, Y Ohira and A Ishihara : Effects of hyperbaric exposure with high oxygen concentration on the physical activity of developing rats., Developmental Neuroscience, Vol.29, No.6, 452-459, 2007.
(要約)
The effects of hyperbaric exposure with high oxygen concentration on the physical activity of developing male rats were investigated. Five-week-old male rats were exposed to an atmospheric pressure of 1.25 with an oxygen concentration of 36.0% for 12 h (7.00-19.00 h) and exercised voluntarily for 12 h (19.00-7.00 h) daily for 8 weeks. The voluntary running activities were compared with those in age-matched rats without hyperbaric exposure. In addition, the properties of the soleus and plantaris muscle fibers and their spinal motoneurons were examined. The voluntary running activities of rats with or without hyperbaric exposure increased during development. However, the mean voluntary running activities were higher in rats with hyperbaric exposure (7,104 m/day) than in those without hyperbaric exposure (4,932 m/day). The oxidative capacities of the soleus and plantaris muscle fibers and their spinal motoneurons increased following hyperbaric exposure. It is suggested that adaptations of neuromuscular units to hyperbaric exposure with high oxygen concentration enhance the metabolism, and thus, the function of neuromuscular units is promoted.
(キーワード)
Animals / Animals, Newborn / Atmospheric Pressure / Body Weight / Male / Motor Activity / Motor Neurons / Muscle Fibers, Skeletal / Muscle, Skeletal / Organ Size / Osmolar Concentration / 酸化と還元 (oxidation and reduction) / Oxygen / Rats / Rats, Wistar / 脊髄 (spinal cord) / Volition
Hang Guo, Ai Saiga, Mikako Sato, Izumi Miyazawa, Makoto Shibata, Yoshihisa Takahata and Fumiki Morimatsu : Royal jelly supplementation improves lipoprotein metabolism in humans., Journal of Nutritional Science and Vitaminology, Vol.53, No.4, 345-348, 2007.
(要約)
Royal jelly (RJ) has several physiological effects and is widely used in commercial medical products and health foods. We examined the effects of RJ supplementation on serum lipoprotein metabolism in humans. Fifteen volunteers were divided into an RJ intake group (n=7) and a control group (n=8). The RJ group took 6 g per day for 4 wk. Their serum total cholesterol (TC) and serum low-density lipoprotein (LDL) decreased significantly compared with those of the control group (p<0.05). There were no significant differences in serum high-density lipoprotein (HDL) or triglyceride concentrations. Moreover, the relationship between the serum cholesterol and lipoprotein levels was investigated. Among the lipoprotein fractions, small very-low-density lipoprotein was decreased (p<0.05) after RJ intake. Our results suggest that dietary RJ decreases TC and LDL by lowering small VLDL levels.
(キーワード)
Adult / Blood Cell Count / Blood Chemical Analysis / Body Composition / Body Mass Index / Body Weight / Cholesterol, HDL / Cholesterol, LDL / Dietary Supplements / Fatty Acids / Female / Hemoglobins / Humans / Lipoproteins / Male / Triglycerides
Eiichiro Onitsuka, Tomoyuki Okumura, Hiroshi Murakami, Norikazu Nishino and Fumiki Morimatsu : A judgment on postmortem aging in longissimus dorsi based on a peptide substrate library., Bioscience, Biotechnology, and Biochemistry, Vol.70, No.12, 2836-2843, 2006.
(要約)
We attempted to develop a method to determine easily and effectively the degree of postmortem aging of pork longissimus dorsi (LD) by measuring the activity of proteases in the LD using fluorogenic peptide substrates. LD was used to measure the change with time in the protease activity detected with these substrates. Determining the variations within the LD muscles, strong positive correlations were found between changes in hardness and fluorescence intensities against Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P<0.005), and strong negative correlations were found between changes in total amounts of free amino acids and Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P<0.001). Negative correlations were also observed between changes in the amounts of free Ala, Arg, Lys, Leu, Met, Phe, and Tyr and the fluorescence intensities against Ala, Arg, Lys, Leu, Met, Phe, and Tyr-MCA respectively (P<0.001).
Ai Saiga, Tomoyuki Okumura, Toshikazu Makihara, Shin-Ichiro Katsuda, Fumiki Morimatsu and Toshihide Nishimura : Action mechanism of an angiotensin I-converting enzyme inhibitory peptide derived from chicken breast muscle., Journal of Agricultural and Food Chemistry, Vol.54, No.3, 942-945, 2006.
(要約)
In a previous study, we isolated the inhibitory peptide (P4 peptide, Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe) for angiotensin I-converting enzyme (ACE) from chicken breast muscle extract possessing hypotensive activity for spontaneously hypertensive rats (SHRs). This study was performed to elucidate the peptide's action mechanisms of inhibiting ACE. Intravenous administration of synthetic P4 peptide resulted in significant drops in the blood pressures of SHRs. As Dixon plots indicate, the P4 peptide showed high affinity toward ACE (K(i) = 11.48 microM) and only 10% of the total amount of the P4 peptide was decomposed. The analyses of the relationship between the ACE inhibitory activity and structure of the P4 peptide clarified that Hyp-Gly-Leu-Hyp-Gly-Phe showed a stronger activity (IC50 = 10 microM) than the P4 peptide (IC50 = 46 microM). When Phe at the C-terminus of the P4 peptide was deleted, IC50 changed to 25000 microM, indicating that Phe at the C-terminus of the peptide is very important for ACE inhibitory activity.
Muneshige Shimizu, Soichi Tanabe, Fumiki Morimatsu, Koji Nagao, Teruyoshi Yanagita, Norihisa Kato and Toshihide Nishimura : Consumption of pork-liver protein hydrolysate reduces body fat in Otsuka Long-Evans Tokushima Fatty rats by suppressing hepatic lipogenesis., Bioscience, Biotechnology, and Biochemistry, Vol.70, No.1, 112-118, 2006.
(要約)
This study was performed to examine the effect of consumption of pork-liver protein hydrolysate (PLH) on body fat accumulation in Otsuka Long-Evans Tokushima Fatty (OLETF) rats as a non-insulin-dependent diabetes mellitus model and in Long-Evans Tokushima Otsuka (LETO) rats as a control. Male 20-week-old OLETF and LETO rats were pair-fed either PLH or casein containing diet for 14 weeks. In the OLETF rats, dietary PLH significantly reduced the growth and weight of fat pad including perirenal and epididymal adipose tissues. Consumption of PLH markedly suppressed hepatic activities of lipogenesis enzymes such as glucose-6-phosphate dehydrogenase and fatty acid synthase and slightly elevated fecal excretion of total fat. In the LETO rats, growth and adipose tissue weight were unaffected by dietary treatment. The results suggest that PLH is a novel ingredient suppressing body fat in genetically obese rats by reducing lipogenesis.
Naoko Sawashita, Aki Naemura, Muneshige Shimizu, Fumiki Morimatsu, Yoshinobu Ijiri and Junichiro Yamamoto : Effect of dietary vegetable and animal proteins on atherothrombosis in mice., Nutrition, Vol.22, No.6, 661-667, 2006.
(要約)
It is believed that vegetable and fish, but not animal, proteins prevent thrombosis. The present study compared the effect of long-term intake of purified vegetable and animal proteins (casein, pork, egg white, chicken, white and red fish, soybean, and potato) and powders from whole vegetable and animal meats (soybean, pork, chicken, and horse mackerel) on thrombotic tendency. Western-style high-fat diets with various proteins were given to mice deficient in apolipoprotein-E and low-density lipoprotein receptor for 8 or 12 wk. Thrombotic tendency was assessed by a laser-induced thrombosis test, severity of atherosclerosis was assessed by morphometric analysis of the aortic lesions, and reactivity of platelets was measured by a shear-induced platelet function test. There was no difference in thrombotic tendency between the vegetable and animal protein diets with the exception of pork protein, which showed a prothrombotic effect. The latter was not observed in animals kept on a high-protein diet. Thrombotic tendency was not affected by preparation of dietary powders from whole pork, chicken, or horse mackerel meats and casein (control), all of which contained the same percentages of protein and lipid. In contrast, soybean powder showed an antithrombotic effect, which was mainly due to an inhibition of atherogenesis rather than to platelet inhibition. Neither the purified animal nor the vegetable protein diet affected thrombotic tendency. Soybean powder inhibited atherothrombosis mainly by inhibiting the atherosclerotic process.
Yasuhiro Suzuki, Tomomi Nakao, Hirohiko Maemura, Mikako Sato, Kazuyuki Kamahara, Fumiki Morimatsu and Kaoru Takamatsu : Carnosine and anserine ingestion enhances contribution of nonbicarbonate buffering., Medicine and Science in Sports and Exercise, Vol.38, No.2, 334-338, 2006.
(要約)
The purpose of the present study was to investigate the effect of supplementation with chicken breast extract (CBEX), which was a rich source of carnosine and anserine, on acid-base balance and performance during intense intermittent exercise. Eight male subjects performed intense intermittent exercise that consisted of 10 x 5-s maximal cycle ergometer sprints with a 25-s recovery period between each sprint. The subjects ingested 190 g of the test soup containing either CBEX or a placebo 30 min before the commencement of exercise. Arterial blood samples were collected at rest and during exercise to estimate the carnosine and anserine concentrations, pH, and bicarbonate concentration ([HCO3-]). Concentrations of anserine and its related amino acid significantly increased 30 min after CBEX supplementation, as compared with their values at rest. However, carnosine did not increase significantly. Following CBEX supplementation, the pH was significantly higher (P < 0.05) at the end of exercise, and [HCO3-] was also significantly higher (P < 0.05) during the latter half of exercise and after exercise. There were no significant differences in the total power and mean power of each set between the CBEX and placebo supplemented groups. Although oral supplementation with CBEX (which is a rich source of carnosine and anserine) increased the contribution of the nonbicarbonate buffering action and decreased bicarbonate buffering action in blood, intense intermittent exercise performance did not improve significantly.
(キーワード)
Adult / 分散分析 (analysis of variance) / Animals / Anserine / Bicarbonates / Blood Gas Analysis / Buffers / Carnosine / Chickens / Cross-Over Studies / Double-Blind Method / Exercise Test / Humans / Hydrogen-Ion Concentration / Male
Ryoji Yamada, Masaki Kozono, Takashi Ohmori, Fumiki Morimatsu and Masahiko Kitayama : Simultaneous determination of residual veterinary drugs in bovine, porcine, and chicken muscle using liquid chromatography coupled with electrospray ionization tandem mass spectrometry., Bioscience, Biotechnology, and Biochemistry, Vol.70, No.1, 54-65, 2006.
(要約)
A simple and rapid method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of 130 veterinary drugs and their metabolites in bovine, porcine, and chicken muscle was developed. The drugs (1 to 10 ng/g, in muscle) were extracted from bovine, porcine, or chicken muscles with acetonitrile-methanol (95:5, v/v), and the extracts were delipidated with n-hexane saturated with acetonitrile. The extracts were evaporated, dissolved with methanol, analyzed by liquid chromatography with gradient elution on a C18 column, and determined by electrospray ionization tandem mass spectrometry. The detection limits ranged from 0.03 to 3 ng/g. The quantitation limits ranged from 0.1 to 10 ng/g. One hundred eleven, 122, and 123 drugs from bovine, porcine, and chicken muscle respectively showed recoveries between 70 and 110%.
Hirohiko Maemura, Kazushige Goto, Toshitsugu Yoshioka, Mikako Sato, Yoshihisa Takahata, Fumiki Morimatsu and Kaoru Takamatsu : Effects of Carnosine and Anserine Supplementation on Relatively High Intensity Endurance Performance, International Journal of Sport and Health Science, Vol.4, 86-94, 2006.
84.
Koji Iwai, Takanori Hasegawa, Yasuki Taguchi, Fumiki Morimatsu, Kenji Sato, Yasushi Nakamura, Akane Higashi, Yasuhiro Kido, Yukihiro Nakabo and Kozo Ohtsuki : Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates., Journal of Agricultural and Food Chemistry, Vol.53, No.16, 6531-6536, 2005.
(要約)
In the present study, we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates. Healthy human volunteers ingested the gelatin hydrolysates (9.4-23 g) from porcine skin, chicken feet, and cartilage after 12 h of fasting. Negligible amounts of the peptide form of hydroxyproline (Hyp) were observed in human blood before the ingestion. After the oral ingestion, the peptide form of Hyp significantly increased and reached a maximum level (20-60 nmol/mL of plasma) after 1-2 h and then decreased to half of the maximum level at 4 h after the ingestion. Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp. In addition, small but significant amounts of Ala-Hyp, Ala-Hyp-Gly, Pro-Hyp-Gly, Leu-Hyp, Ile-Hyp, and Phe-Hyp were contained.
Akihiko Ishihara, Fuminori Kawano, Tomonori Okiura, Fumiki Morimatsu and Yoshinobu Ohira : Hyperbaric exposure with high oxygen concentration enhances oxidative capacity of neuromuscular units., Neuroscience Research, Vol.52, No.2, 146-152, 2005.
(要約)
The effects of hyperbaric exposure with high oxygen concentration on spinal motoneurons and the skeletal muscle fibers that they innervate were investigated. Five-week-old male rats were exposed to a hyperbaric (1.25 atmospheric pressure) environment with a high oxygen concentration (35.0%) for 6h daily. The number, cell body size, and oxidative enzyme activity of motoneurons innervating the soleus and plantaris muscles were examined after 8 weeks of hyperbaric exposure. In addition, the fiber type distribution, cell size, and oxidative enzyme activity of the slow soleus and fast plantaris muscles were examined. The oxidative enzyme activity of alpha motoneurons innervating the soleus and plantaris muscles increased after hyperbaric exposure, irrespective of their cell body sizes. The percentage of high-oxidative fibers in the soleus and plantaris muscles increased after hyperbaric exposure. The oxidative enzyme activity of all types of fibers in the soleus and plantaris muscles increased after hyperbaric exposure. It is concluded that hyperbaric exposure with high oxygen concentration enhances the oxidative capacity of neuromuscular units.
Masaki Hashida, Katsuyuki Miyatake, Yoshiharu Okamoto, Kotaro Fujita, Takayuki Matumoto, Fumiki Morimatsu, Koji Sakamoto and Saburo Minami : Synergistic Effects of D-Glucosamine and Collagen Peptide on Healing Experimental Cartilage Injury, Macromolecular Bioscience, Vol.3, No.10, 596-603, 2002.
96.
Soichi Tanabe, Yoko Kobayashi, Yoshihisa Takahata, Fumiki Morimatsu, Rumiko Shibata and Toshihide Nishimura : Some human B and T cell epitopes of bovine serum albumin, the major beef allergen, Biochemical and Biophysical Research Communications, Vol.293, No.5, 1348-1353, 2002.
(要約)
Bovine serum albumin (BSA) is the major beef allergen. Since IgE and T cell recognitions are central to the specific immune response to allergens, the identification and immunologic characterization of B and T cell epitopes of BSA represent important steps in the development of treatments for beef allergy. Prior to our experiments, we hypothesized that BSA-specific antibodies and T cells react primarily with sequential epitopes in which the amino acid sequences differ greatly between bovine and human albumin. To clarify this hypothesis, 16 peptides corresponding to such regions were synthesized as candidate epitopes. Among them, at least two regions, aa336-345 and aa451-459, were found to be B cell (IgE-binding) epitopes. In inhibition ELISA experiments, EYAV (aa338-341) and LILNR (aa453-457) bound to patient IgE antibodies and were found to be the cores of the IgE-binding epitopes. Three regions, DDSPDLPKLKPDPNTLC (aa107-123), PHACYTSVFDKLKHLVDEP (aa364-382), and LSLILNRLC (aa451-459), were found to induce T cell proliferation in more than half of the patients tested. Of interest was that these three regions were also recognized by B cells. Information concerning human B and T cells epitopes can contribute greatly to the elucidation of the etiology of beef allergy.
Shin-ichiro Katsuda, Yumi Waseda, Fumiki Morimatsu, Yasuki Taguichi, Masamitsu Hasegawa, Michiko Ito, Shigeko Takaichi, Ryoji Yamada, Yuji Furukawa and Tuyoshi Shimizu : Papain-Hydrolyzed Pork Meat Reduces Serum Cholesterol level and Premature Atherosclerosis in Dietary-Induced Hypercholesterolemic Rabbit, Journal of Nutritional Science and Vitaminology, Vol.46, No.4, 180-187, 2000.
(要約)
The effects of the low-molecular-weight fraction of papain-hydrolyzed pork meat (LMF) on the plasma cholesterol level and the generation of atherosclerosis were studied in rabbits fed a cholesterol-enriched diet. In LMF-fed rabbits, the plasma and liver cholesterol concentrations were both significantly lower (p<0.0 1) than in rabbits fed untreated pork meat (PM). Similarly, the cholesterol concentrations of the chylomicron and VLDL fractions were significantly lower in LMF-fed rabbits than in rabbits fed PM. Deposition of lipid in transverse sections of the aortic arch was significantly less in rabbits fed LMF than in those fed PM. Electron microscopic studies revealed preventive effects against premature atherosclerotic lesions in the aorta of rabbits fed LME These results indicate that LMF has a hypocholesterolemic action and preventive effects against premature atherosclerosis.
森松 文毅 : 高脂血症と食肉製品(大豆蛋白質), BIO Clinica, Vol.11, 511-513, 1996年.
107.
Fumiki Morimatsu, Michiko Ito, Budijanto Slamet, Itaru Watanabe, Yuji Furukawa and Shuichi Kimura : Plasma cholesterol-suppressing effect of papain-hydrolyzed pork meat in rats fed hypercholesterolemic diet., Journal of Nutritional Science and Vitaminology, Vol.42, No.2, 145-153, 1996.
(要約)
The effects of papain-hydrolyzed pork meat on plasma and liver cholesterol levels were studied in rats fed a cholesterol-enriched diet. In rats fed the low-molecular-weight fraction of papain-hydrolyzed pork meat, the plasma cholesterol concentration, more particularly the VLDL and LDL cholesterol concentrations, were significantly lower (p < 0.01) than in the rats fed untreated pork meat or soybean protein. Feeding with this fraction rather than with untreated pork meat also led to a significantly lower liver cholesterol concentration (p < 0.01) and increased fecal excretion of neutral and acidic steroids. The low-molecular-weight fraction contained peptides with molecular weights of 3,000 or less and had an amino acid composition similar to that of pork meat itself. This study suggests that peptides produced by papain-hydrolysis of pork meat have a hypocholesterolemic activity through their interference with the steroid absorption process.
Suh-Ching Yang, Michiko Ito, Fumiki Morimatsu, Yuji Furukawa and Shuichi Kimura : Effects of amino acids on alcohol intake in stroke-prone spontaneously hypertensive rats., Journal of Nutritional Science and Vitaminology, Vol.39, No.1, 55-61, 1993.
(要約)
Examination was made of the effects of amino acids on alcohol intake in stroke-prone spontaneously hypertensive rats (SHRSP). The animals were divided into two groups according to dietary protein level, normal (15%) and low (5%). The two groups were further divided into two groups according to whether they selected the ethanol solution containing amino acids (non-amino acid group and amino acid group). The 5% ethanol solutions with and without various amino acids were prepared in a water-supplying tube to which the animals had free access for 40 days. The 5% ethanol solution intake in rats fed the normal protein diet was higher than that of the low protein group. Regardless of dietary protein level, 5% ethanol solution intake increased in the amino acid group. Intake of the 5% ethanol solution containing 100 mM L-proline, 100 mM L-lysine, and 100 mM L-threonine was large. For the amino acid group of rats fed normal protein and low protein, plasma glutamate oxalacetate transaminase (GOT) activity was significantly reduced. It is suggested that the alcohol intake may increase by adding amino acid to the alcohol solution.
S Kimura, C H. Kim, I M. Ohtomo, Y Yokomukai, M Komai and Fumiki Morimatsu : Nutritional Studies of the Roles of Dietary Protein levels and Umami in the Preference Response to Sodium Chloride for Experimental Animals, Physiology & Behavior, Vol.49, No.5, 997-1002, 1991.
(要約)
Previously we found that appetite or preference for sodium chloride depends not only on a genetic factor, but also on the nutritional status, i.e., dietary protein levels. To determine whether all kinds of protein had reducing effects on sodium chloride intake, the effect of dietary protein source on appetite or preference for sodium chloride was studied. It was found that the effect depended not only on protein levels, but also on the protein sources, i.e., amino acid patterns of diets. On the other hand, the turnover time of gustatory taste bud cells was measured by the pulse-labeled method using 3H-thymidine. It was found that the turnover time of gustatory cells in the taste buds of rats fed a low-protein diet was longer than in rats fed a high-protein diet. This suggested that some reduction of receptor functions may occur with low-protein diet. It was found that the dietary protein levels and umami also modified appetite or preference for alcohol solutions.
R. Sakata, T. Oshida, T. Okiura, T. Okumura, Fumiki Morimatsu and R. Yamada : Japaner schaetzen hohe Fleichqualitaet Vergleichende Beurteilung von importiertem aus tralischen und US-Rindfleisch, Fleischwirtschaft, Vol.85, No.2, 16-18, Sep. 2005.
Maki Hirata, Matsuoka Miki, Hashimoto Takuma, Oura Takamichi, Ohnuki Yo, Yoshida Chika, Minemura Ayaka, Miura Daiki, Oka Kentaro, Takahashi Motomichi and Fumiki Morimatsu : Effect of Clostridium butyricum MIYAIRI 588 supplementation on the intestinal microbiota and meat quality of fattening pigs., 68th International Congress of Meat Science and Technology (ICoMST2022), Kobe, Aug. 2022.
2.
Fumiki Morimatsu : Current status of processed meat products in Japan, Fecoprod Simposium, Asunción, Paraguay, Jul. 2017.
3.
M. Sato and Fumiki Morimatsu : Application of Carnosine and Anserine in Functional Food, 国際カルノシン・アンセリン学会2014要旨集, Tokyo, Aug. 2014.
4.
K. Takeshita, Y. Terao, Y. Nishiyama, N. Morishita, T. Matsumoto and Fumiki Morimatsu : Development and evaluation of a nucleic acid lateral flow assay for detection of STEC in foods, 127th AOAC International annual meeting, Chicago, Aug. 2013.
5.
Y. Nishiyama, T. Yonekita, E. Hojo, K. Kamiya, N. Morishita, T. Matsumoto and Fumiki Morimatsu : Immunochromatographic assays for detection of allergenic substances in processed foods, 127th AOAC International annual meeting, Chicago, Aug. 2013.
6.
K. Kadooka, T. Matsumoto, Fumiki Morimatsu and Y. Katakura : Carnosine indeces Caco-2 cells to secrete neurotrophic factors and activate SH-SY5Y cells, Keystone Symposia Conference, Santa Fe,New Mexico, USA, Oct. 2012.
7.
Y. Nishiyama, Y. Terao, N. Morishita, T. Matsumono, Fumiki Morimatsu and S. Igimi : Development of a novel nucleic acid lateral flow assay for detection of Listeria monocytogenes in food, 126th AOAC International Annual Meeting & Exposition, Las Vegas, Oct. 2012.
8.
Y. Nishiyama, K. Kamiya, T. Yonekita, N. Morishita, T. Matsumono and Fumiki Morimatsu : Evaluation of an immunochromatographic assay for simple and rapid detection of Escherichia coli O157, 126th AOAC International Annual Meeting & Exposition, Las Vegas, Oct. 2012.
9.
E. Houjo, K. Kamiya, T. Yonekita, Y. Terao, N. Morishita, T. Matsumoto, Fumiki Morimatsu and O. Kawamura : Single-laboratory validation of an ELISA test kit for the detection of total aflatoxins in corn by comparison with HPLC, 126th AOAC International Annual Meeting & Exposition, Las Vegas, Oct. 2012.
K. Ichie, Y. Taguchi, Y. Takahata, S. Toki, Fumiki Morimatsu, T. Yamanaka, T. Kumagai, Y. Wataya, K. Kimura, Y. Tanaka, K. Ikeda, A. Umetsu, R. Shibata, J. Kurisaki and R. Yamada : Low Allergic gelatin Prepared from Chicken Cartilage by Acid Processing., 45th ICoMST 1999. Congress Proceeding, 714-715, Jul. 1999.
12.
S. Toki, K. Ichie, Y. Taguchi, Y. Takahata, Fumiki Morimatsu, T. Yamanaka, T. Kumagai, Y. Wataya, K. Kimura, Y. Tanaka, K. Ikeda, A. Umetsu, R. Shibata, J. Kurisaki and R. Yamada : Allergenic Properties of Heat-Denatured Collagen are Different Depending on the Collagen Type and the Source., 45th ICoMST 1999. Congress Proceeding, 712-713, Jul. 1999.
13.
K. Fujita, Y. Takahata, Fumiki Morimatsu, R. Shibata, J. Kurisaki and R. Yamada : Determination and Elimination of Allergens in Beef and Chicken., 45th ICoMST 1999. Congress Proceeding, 710-711, Jul. 1999.
14.
T. Matsumoto, A. Ametani, S. Hachimura, A. Iwata, Y. Taguchi, K. Fujita, Fumiki Morimatsu, T. Shigehisa, R. Yamada and S. Kaminogawa : Intransal Adoministration of Denatured Type- Collagen and Its Fragments can Suppresses Collagen-Induced Arthritis., 45th ICoMST 1999. Congress Proceeding,, 703-704, Jul. 1999.
15.
Y. Taguchi, K. Urashima, K. Fujita, Fumiki Morimatsu, T. Shigehisa, S. Kaminogawa and R. Yamada : Intransal Administration of Type- Collagen Suppresses Collagen-Induced Arthritis in Mice and Enhances the Effect of an Anti-Inflammatory Drug, 45th ICoMST 1999. Congress Proceeding,, 702-703, Jul. 1999.
16.
N. Shioda, K. Urashima, M. Tanaka, Fumiki Morimatsu, K. Tezuka and R. Yamada : Oral Administration of Chicken Gelatin Peptide Improves Moisture Retention of Skin, 45th ICoMST 1999. Congress Proceeding, 686-687, Jul. 1999.
17.
K. Urashima, Y. Taguchi, Fumiki Morimatsu, Y. Furukawa and R. Yamada : Papain-Digested Pork Reduces Serum Cholesterol Levels in Rats, 45th ICoMST 1999. Congress Proceeding, 696-697, Jul. 1999.
18.
M. Sato, T Ohmori, Fumiki Morimatsu and R. Yamada : Pork Hydrolyzed Stimulates Alcohol Metabolism in Rats and Man, 45th ICoMST 1999. Congress Proceeding, 680-681, Jul. 1999.
19.
M. Shimane, K. Fujita, Y. Takahata, Fumiki Morimatsu, F. Furukawa, M. Takigawa and R. Yamada : Alleviation of Atopic Dermatitis with γ-Linolenic Acid-Containing Sausage, 45th ICoMST 1999. Congress Proceeding, 670-671, Jul. 1999.
20.
R. Yamada, J. Kurisaki, R. Sshibata, Y. Takahata, Fumiki Morimatsu and K. Mizumachi : Specificity of the IgE Antibodies from Allergic Patients Sensitive to Meat Products, 43rd ICoMST 1997 Congress Proceeding, 766-777, Jun. 1997.
21.
楊 素卿, 伊藤 道子, 森松 文毅, 古川 勇次, 木村 修一 : ラットのアルコール代謝に及ぼすアミノ酸の影響, Proceeding of the 26th Symposium on Taste and Smell, 153-156, 1993年5月.
22.
Fumiki Morimatsu, M. Kodama, M. Komai, M. Ito and S. Kimura : Effects of Dietary Protein Source on the Palatability to Salty Taste in Rats, Proceeding of the 22nd Japanese Symposium on the Taste and Smell, 45-48, May 1988.
山崎 栄樹 and Fumiki Morimatsu : Testing accuracy control in academic diagnostic center accredited by ISO/IEC 17025, and application of the accreditation to business., イノベーションジャパン 2017, Aug. 2017.
Y. Muneta, Y. Yakabe, H. Iseki, H. Kobayashi, Y. Takahagi, Fumiki Morimatsu, H. Sinkai and H. Uenishi : Serum interleukin-18(IL-18) concentration is a useful indicator to evaluate hygiene status of pig production farm, 18th Federation of Asian Veterinary Associations Congress, Nov. 2014.