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Tokushima UniversityGraduate School of Technology, Industrial and Social SciencesDivision of Bioscience and BioindustryFood ScienceFood Science and Technology
Tokushima UniversityFaculty of Bioscience and Bioindustry
Tokushima UniversityFaculty of Integrated Arts and SciencesDepartment of Civil and Environmental StudiesSymbiotic Environmental Studies
Tokushima UniversityGraduate School of Integrated Arts and SciencesRegional ScienceEnvironmental Symbiosis Studies
Tokushima UniversityResearch ClustersResearch Clusters (Registered)2004017 食品成分による食品用器材への微生物初期付着抑制と応用 ―食環境の微生物制御(バイオフィルム形成抑制)を目指して―
Tokushima UniversityFaculty of Bioscience and BioindustryDepartment of Bioscience and BioindustryFood Science
Tokushima UniversityResearch ClustersResearch Clusters (Registered)2004011 生物の代謝酵素を利用した環境に優しい 次世代地盤改良技術「バイオグラウト」の開発
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Field of Study

Enzyme Chemistry

Subject of Study

極限環境微生物酵素の機能・構造・応用 (超好熱アーキア, アミノ酸脱水素酵素, アミノ酸ラセマーゼ, タンパク質結晶化, X線結晶構造解析)

Book / Paper

Academic Paper (Judged Full Paper):

1. Rumana Yesmin Hasi, Dai Majima, Katsuya Morito, Hanif Ali, Kentaro Kogure, Meera Nanjundan, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities.,
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, Vol.1152, 122213, 2020.
(Tokushima University Institutional Repository: 115195,   DOI: 10.1016/j.jchromb.2020.122213,   PubMed: 32615533)
2. Ali Hanif, Ryouhei Yamashita, Morishige Jun-ichi, Morito Katsuya, Kakiuchi Naoya, Junji Hayashi, Mutsumi Aihara, Ryushi Kawakami, Koichiro Tsuchiya and Tamotsu Tanaka :
Massspectrometric analysis of sphingomyelin with N-alfa-hydroxy fatty acyl residue in mouse tissues,
Lipids, 2020.
(DOI: 10.1002/lipd.12285)
3. Ryushi Kawakami :
超好熱アーキアのFAD依存性脱水素酵素複合体及びPLP依存性アミノ酸ラセマーゼの研究,
ビタミン, Vol.93, No.12, 531-541, 2019.
(Tokushima University Institutional Repository: 115046,   CiNii: 120006877058)
4. Hasi Yesmin Rumana, Miyagi Makoto, KIDA Takashi, Tatsuya Fukuta, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Quantitative Analysis of Glycosylinositol Phosphoceramide and Phytoceramide 1-Phosphate in Vegetables,
Journal of Nutritional Science and Vitaminology, Vol.65, No.Supplement, S175-S179, 2019.
(Tokushima University Institutional Repository: 115197,   DOI: 10.3177/jnsv.65.S175,   PubMed: 31619623,   Elsevier: Scopus)
5. Hasi Yesmin Rumana, Makoto Miyagi, Katsuya Morito, Toshiki Ishikawa, Maki Kawai-Yamada, Hiroyuki Imai, Tatsuya Fukuta, Kentaro Kogure, Kaori Kanemaru, Junji Hayashi, Ryushi Kawakami and Tamotsu Tanaka :
Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation,
The Journal of Biochemistry, Vol.166, No.5, 441-448, 2019.
(Tokushima University Institutional Repository: 113685,   DOI: 10.1093/jb/mvz056,   PubMed: 31504617,   Elsevier: Scopus)
6. Ryushi Kawakami, Tatsuya Ohshida, Haruhiko Sakuraba and Toshihisa Ohshima :
A novel PLP-dependent Alanine/Serine racemase from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3,
Frontiers in Microbiology, 2018.
(Tokushima University Institutional Repository: 114287,   DOI: 10.3389/fmicb.2018.01481,   Elsevier: Scopus)
7. Ryushi Kawakami, Haruhiko Sakuraba, Ohmori Taketo and Toshihisa Ohshima :
First characterization of an archaeal amino acid racemase with broad substrate specificity from the hyperthermophile Pyrococcus horikoshii OT-3 induced by D-amino acids,
Journal of Bioscience and Bioengineering, Vol.124, No.1, 23-27, 2017.
(Tokushima University Institutional Repository: 111977,   DOI: 10.1016/j.jbiosc.2017.02.004,   PubMed: 28343923,   Elsevier: Scopus)
8. Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru :
Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin.,
European Food Research and Technology, Vol.243, No.12, 2147-2153, 2017.
(Tokushima University Institutional Repository: 111019,   DOI: 10.1007/s00217-017-2915-1)
9. Tatsuya Ohshida, Junji Hayashi, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase,
Protein Expression and Purification, Vol.126, 62-68, 2016.
(Tokushima University Institutional Repository: 111972,   DOI: 10.1016/j.pep.2016.05.009,   PubMed: 27215670,   Elsevier: Scopus)
10. Ryushi Kawakami, Taketo Ohmori, Haruhiko Sakuraba and Toshihisa Ohshima :
Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids,
Amino Acids, Vol.47, No.8, 1579-1587, 2015.
(DOI: 10.1007/s00726-015-2001-6,   PubMed: 25963389,   Elsevier: Scopus)
11. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis,
Bioscience, Biotechnology, and Biochemistry, Vol.78, No.12, 2045-2050, 2014.
(DOI: 10.1080/09168451.2014.946394,   PubMed: 25126984,   Elsevier: Scopus)
12. Ryushi Kawakami, Chiaki Noguchi, Marie Higashi, Haruhiko Sakuraba and Toshihisa Ohshima :
Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes.,
Applied Microbiology and Biotechnology, Vol.97, No.8, 3419-3427, 2013.
(DOI: 10.1007/s00253-012-4201-2,   PubMed: 22752365)
13. Haruhiko Sakuraba, Takenori Satomura, Ryushi Kawakami, Kwang Kim, Yusuke Hara, Kazunari Yoneda and Toshihisa Ohshima :
Crystal structure of novel dye-linked L-proline dehydrogenase from hyperthermophilic archaeon Aeropyrum pernix.,
The Journal of Biological Chemistry, Vol.287, No.24, 20070-20080, 2012.
(DOI: 10.1074/jbc.M111.319038,   PubMed: 22511758)
14. Takenori Satomura, Akihiro Hiraki, Tomoyuki Kawai, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Expression, purification, crystallization and preliminary X-ray diffraction analysis of a galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.68, No.Pt 3, 330-332, 2012.
(DOI: 10.1107/S1744309112003880,   PubMed: 22442236)
15. Ryushi Kawakami, Takenori Satomura, Haruhiko Sakuraba and Toshihisa Ohshima :
L-proline dehydrogenases in hyperthermophilic archaea: distribution, function, structure, and application.,
Applied Microbiology and Biotechnology, Vol.93, No.1, 83-93, 2011.
(DOI: 10.1007/s00253-011-3682-8,   PubMed: 22089387)
16. Takenori Shibahara, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.67, No.Pt 11, 1425-1427, 2011.
(DOI: 10.1107/S1744309111036098,   PubMed: 22102248)
17. Haitao Zheng, Leyi Lin, Yosuke Okezaki, Ryushi Kawakami, Haruhiko Sakuraba, Toshihisa Ohshima, Keiichi Takagi and Shin-Ichiro Suye :
Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrodes modified by multi-walled carbon nanotubes.,
Beilstein Journal of Nanotechnology, Vol.1, 135-141, 2010.
(DOI: 10.3762/bjnano.1.16,   PubMed: 21977403)
18. Ryushi Kawakami, Masaki Oyama, Haruhiko Sakuraba and Toshihisa Ohshima :
The unique kinetic behavior of the very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum.,
Bioscience, Biotechnology, and Biochemistry, Vol.74, No.4, 884-887, 2010.
(DOI: 10.1271/bbb.90925,   PubMed: 20378971,   CiNii: 10027554364)
19. Ryushi Kawakami, Haruhiko Sakuraba, Shuichiro Goda, Hideaki Tsuge and Toshihisa Ohshima :
Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix.,
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol.1794, No.10, 1496-1504, 2009.
(DOI: 10.1016/j.bbapap.2009.06.014,   PubMed: 19555779)
20. Haruhiko Sakuraba, Kazunari Yoneda, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
Structure of a D-tagatose 3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.65, No.Pt 3, 199-203, 2009.
(DOI: 10.1107/S1744309109002115,   PubMed: 19255464)
21. Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
A novel flavin adenine dinucleotide (FAD) containing d-lactate dehydrogenase from the thermoacidophilic crenarchaeota Sulfolobus tokodaii strain 7: purification, characterization and expression in Escherichia coli.,
Journal of Bioscience and Bioengineering, Vol.106, No.1, 16-21, 2008.
(DOI: 10.1263/jbb.106.16,   PubMed: 18691525)
22. Haruhiko Sakuraba, Kazunari Yoneda, Kumiko Yoshihara, Kyoko Satoh, Ryushi Kawakami, Yoshihiro Uto, Hideaki Tsuge, Katsuyuki Takahashi, Hitoshi Hori and Toshihisa Ohshima :
Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase.,
Applied and Environmental Microbiology, Vol.73, No.22, 7427-7434, 2007.
(DOI: 10.1128/AEM.01101-07,   PubMed: 17905878)
23. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Gene cloning and characterization of the very large NAD-dependent L-glutamate dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil,
Journal of Bacteriology, Vol.189, No.15, 5626-5633, 2007.
(DOI: 10.1128/JB.00496-07,   PubMed: 17526698)
24. Toshihisa Ohshima, Ryushi Kawakami, Yuichiro Kanai, Shuichiro Goda and Haruhiko Sakuraba :
Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii.,
Protein Expression and Purification, Vol.54, No.1, 73-78, 2007.
(DOI: 10.1016/j.pep.2007.02.013,   PubMed: 17407821)
25. Yoneda Kazunari, Ryushi Kawakami, Tagashira Yuya, Haruhiko Sakuraba, Shuichiro Goda and Toshihisa Ohshima :
The first archaeal l-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: gene cloning and enzymological characterization,
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol.1764, No.6, 1087-1093, 2006.
(DOI: 10.1016/j.bbapap.2006.04.006,   PubMed: 16731057)
26. Zheng Haitao, Hirose Yutaka, Kimura Tomokazu, Suye Shin-ichiro, Hori Teruo, Katayama Hideo, Arai3 Jun-ichiro, Ryushi Kawakami and Toshihisa Ohshima :
L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator,
Science and Technology of Advanced Materials, Vol.7, No.3, 243-248, 2006.
(DOI: 10.1016/j.stam.2005.12.010)
27. Shuichiro Goda, Masaki Kojima, Yoshimi Nishikawa, Chizu Kujo, Ryushi Kawakami, Seiki Kuramitsu, Haruhiko Sakuraba, Yuzuru Hiragi and Toshihisa Ohshima :
Intersubunit Interaction Induced by Subunit Rearrangement Is Essential for the Catalytic Activity of the Hyperthermophilic Glutamate Dehydrogenase from Pyrobaculum islandicum.,
Biochemistry, Vol.44, No.46, 15304-15313, 2005.
(DOI: 10.1021/bi050478l,   PubMed: 16285734)
28. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Hideo Ago, Masashi Miyano, Kenji Aki, Nobuhiko Katunuma and Toshihisa Ohshima :
Crystal Structure of a Novel FAD-, FMN-, and ATP-containing L-Proline Dehydrogenase Complex from Pyrococcus horikoshii,
The Journal of Biological Chemistry, Vol.280, No.35, 31045-31049, 2005.
(DOI: 10.1074/jbc.C500234200,   PubMed: 16027125)
29. Yasuhiro Shimizu, Haruhiko Sakuraba, Ryushi Kawakami, Shuichiro Goda, Yutaka Kawarabayasi and Toshihisa Ohshima :
L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3, --- gene cloning and enzymatic characterization ---,
Extremophiles, Vol.9, No.4, 317-324, 2005.
(DOI: 10.1007/s00792-005-0447-2,   PubMed: 15902509,   CiNii: 80017783245)
30. Ryushi Kawakami, Haruhiko Sakuraba, Hideaki Tsuge, Shuichiro Goda, Nobuhiko Katunuma and Toshihisa Ohshima :
A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3,
The FEBS Journal, Vol.272, No.16, 4044-4054, 2005.
(DOI: 10.1111/j.1742-4658.2005.04810.x,   PubMed: 16098188)
31. Haruhiko Sakuraba, Ryushi Kawakami and Toshihisa Ohshima :
First Archaeal Inorganic Polyphosphate/ATP-Dependent NAD Kinase, from Hyperthermophilic Archaeon Pyrococcus horikoshii, --- Cloning, Expression, and Characterization ---,
Applied and Environmental Microbiology, Vol.71, No.8, 4352-4358, 2005.
(DOI: 10.1128/AEM.71.8.4352-4358.2005,   PubMed: 16085824)
32. Ryushi Kawakami, Haruhiko Sakuraba, Shintaro Kamohara, Shuichiro Goda, Yutaka Kawarabayasi and Toshihisa Ohshima :
Oxidative stress response in an anaerobic hyperthermophilic archaeon, --- presence of a functional peroxiredoxin in Pyrococcus horikoshii ---,
The Journal of Biochemistry, Vol.136, No.4, 541-547, 2004.
(DOI: 10.1093/jb/mvh157,   PubMed: 15625325)
33. Haruhiko Sakuraba, Ryushi Kawakami, Hajime Takahashi and Toshihisa Ohshima :
Novel Archaeal Alanine:Glyoxylate Aminotransferase from Thermococcus litoralis,
Journal of Bacteriology, Vol.186, No.16, 5513-5518, 2004.
(DOI: 10.1128/JB.186.16.5513-5518.2004,   PubMed: 15292154)
34. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex,
Extremophiles, Vol.8, No.2, 99-108, 2004.
(DOI: 10.1007/s00792-003-0368-x,   PubMed: 15064976)
35. Haruhiko Sakuraba, Hideaki Tsuge, Ikuko Shimoya, Ryushi Kawakami, Shuichiro Goda, Yutaka Kawarabayasi, Nobuhiko Katunuma, Hideo Ago, Masashi Miyano and Toshihisa Ohshima :
The First Crystal Structure of Archaeal Aldolase: Unique Tetrameric Structure of 2-Deoxy-D-ribose-5-phosphate Aldolase from Hyperthermophilic Archaeon Aeropyrum pernix,
The Journal of Biological Chemistry, Vol.278, No.12, 10799-10806, 2003.
(DOI: 10.1074/jbc.M212449200,   PubMed: 12529358)
36. Haruhiko Sakuraba, Issei Yoshioka, Shinji Koga, Mamoru Takahashi, Yuki Kitahama, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
ADP-Dependent Glucokinase/Phosphofructokinase, a Novel Bifunctional Enzyme from the Hyperthermophilic Archaeon Methanococcus jannaschii,
The Journal of Biological Chemistry, Vol.277, No.15, 12495-12498, 2002.
(DOI: 10.1074/jbc.C200059200,   PubMed: 11856730,   CiNii: 80015273383)
37. Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Dye-linked D-Proline Dehydrogenase from Hyperthermophilic Archaeon Pyrobaculum islandicum Is a Novel FAD-dependent Amino Acid Dehydrogenase,
The Journal of Biological Chemistry, Vol.277, No.15, 12861-12867, 2002.
(DOI: 10.1074/jbc.M112272200,   PubMed: 11823469,   CiNii: 80015273429)
38. Haruhiko Sakuraba, Takenori Satomura, Ryushi Kawakami, Sanae Yamamoto, Yutaka Kawarabayasi, Hisasi Kikuchi and Toshihisa Ohshima :
L-Aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing,
Extremophiles, Vol.6, No.4, 275-281, 2002.
(DOI: 10.1007/s00792-001-0254-3,   PubMed: 12215812)
39. Haruhiko Sakuraba, Yoshinori Takamatsu, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
Purification, Characterization, and Application of a Novel Dye-Linked L-Proline Dehydrogenase from a Hyperthermophilic Archaeon, Thermococcus profundus,
Applied and Environmental Microbiology, Vol.67, No.4, 1470-1475, 2001.
(DOI: 10.1128/AEM.67.4.1470-1475.2001,   PubMed: 11282592)

Review, Commentary:

1. Haruhiko Sakuraba, Shuichiro Goda, Ryushi Kawakami, Toshihisa Ohshima, Hideaki Tsuge and 勝沼 信彦 :
Molecular Strategy for Thermostability of Hyperthermophilic Protein,
化学と生物, Vol.44, No.5, 305-312, May 2006.
(DOI: 10.1271/kagakutoseibutsu1962.44.305,   CiNii: 10018130564)
2. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, 安藝 謙嗣, 勝沼 信彦 and Toshihisa Ohshima :
Novel diflavin-containing dehydrogenase family : FAD, FMN and ATP-containing L-proline dehydrogenase,
Seikagaku, Vol.78, No.1, 41-45, Jan. 2006.
(PubMed: 16491912,   CiNii: 10017148546)
3. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアの新規フラビン含有アミノ酸脱水素酵素の機能開発,
酵素工学ニュース, Vol.54, 31-36, Oct. 2005.

Proceeding of International Conference:

1. Fukuda J., Yoneda K., Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Crystallization and Chracterization of L-lysine Dehydrogenase (LysDH) from Hyperthermophilic Archaeon yrococcus horikoshii,
The first joint Symposium on life between Pusan National Univeristy and Kyushu University, Fukuoka, Feb. 2007.
2. Yoneda K., Ryushi Kawakami, Tagashira Y., Haruhiko Sakuraba, Shuichiro Goda, Hideaki Tsuge, Katunuma N. and Toshihisa Ohshima :
The first crystal structure of an archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus,
EXTREMOPHILES 2006 CONFERENCE, Brest, Sep. 2006.
3. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Kenji Aki, Nobuhiko Katunuma, Hideo Ago, Masashi Miyano and Toshihisa Ohshima :
The Crystal Structure of L-proline Dehydrogenase in a Hyperthermophilic Archaeon,
XX Congress of the International Union of Crystallography, Vol.27, No.50, C198, Florence, Italy, Aug. 2005.
4. Toshihisa Ohshima, Ryushi Kawakami and Haruhiko Sakuraba :
Gene and primary stuructures, and properties of a novel type of dye-linked FAD-containing L-proline dehydrogenase complex from the hyperthremophilic archaeon Thermococcus profundus,
The eighth China-Japan-Korea joint symposium on enzyme engineering, 43-44, Hangzhou, China, Oct. 2004.
5. Shin-ichiro Suye, Yutaka Hirose, Tomokazu Kimura, Haitao Zheng, Teruo Hori, Hideo Katayama, Ryushi Kawakami and Toshihisa Ohshima :
Amperometric DNA sensor using gold electrode modified with polymeric mediator and thermostable dehydrogenase by layer-by-layer adsorption,
Nano and Giga Challenges in Microelectronics Symposium and Summer School Reserch and Development Opportunities, Cracow, Sep. 2004.
6. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Kazunari Yoneda, Hideo Ago, Masashi Miyano, Kenji Aki, Nobuhiko Katunuma and Toshihisa Ohshima :
Crystal structure of an unique FAD and FMN containing enzyme; Dye-linked L-proline dehydrogenase from Pyrococcus horikoshii,
The 1st Pacific-Rim International Conference on Protein Science, Yokohama, Apr. 2004.
7. Ryushi Kawakami, Hideaki Tsuge, Shuichiro Goda, Haruhiko Sakuraba, Nobuhiko Katunuma and Toshihisa Ohshima :
Novel dye-linked L-proline dehydrogenase from anaerobic hyperthermophilic archaea, Thermococcus profundus and Pyrococcus horikoshii OT-3,
The 1st Pacific-Rim International Conference on Protein Science, Yokohama, Apr. 2004.
8. Shuichiro Goda, Haruhiko Sakuraba, Mohammad W Bhuiya, Ryushi Kawakami, Seiki Kuramitsu, Yuzuru Hiragi and Toshihisa Ohshima :
Heat and Urea Induced Activations by Subunit Rearrangement of Hyperthermophilic Glutamate Dehydrogenase Produced in Escherichia Coli,
Thermophiles 2003, Vol.P2.17, 143, Exeter, United Kingom, Sep. 2003.
9. Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Novel dye-linked L-prorine dehydrogenase from a hyperthemophilic archaeon, Thermococcus profundus, --- Subunit structure, gene structure and catalytic properties ---,
9th International Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, Jul. 2002.
10. Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Purification and characterization of dye-linked D-proline dehydrogenase from hyperthermophilic archaeon Pyrobaculum islandicum,
International Congress on Biocatalysis, Hamburg, Germany, Jun. 2002.
11. Toshihisa Ohshima, Haruhiko Sakuraba, Takenori Satomura and Ryushi Kawakami :
Two novel dye-linked proline dehydrogenases from anaerobic hyperthermophilic archaea: Their function, structure and application,
Japan-Italy Symposium New Trends in Enzyme Science and Technology, Kyoto, Nov. 2001.

Proceeding of Domestic Conference:

1. Ryushi Kawakami, Junji Hayashi, 木下 千夏, 河瀬 智紀 and 佐藤 樹夫 :
超好熱アーキア Thermococcus litoralis DSM5473 のアミノ酸ラセマーゼ BAR2 の機能解析,
日本ビタミン学会第 72 回大会, Jun. 2020.
2. Tamotsu Tanaka, Rumana Yesmin Hasi, Katsuya Morito, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, 今井 博之 and 石川 寿樹 :
グリコシルイノシトール ホスホセラミドの単離法の開発,
第12回セラミド研究会学術集会, Oct. 2019.
3. Tamotsu Tanaka, Katsuya Morito, Rumana Yesmin Hasi, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, 若山 睦, 近藤 千恵子, Tatsuya Fukuta and Kentaro Kogure :
食品素材に含まれるセラミドの簡便な定量方法,
日本脂質栄養学会第28回大会, Sep. 2019.
4. Ryushi Kawakami, Nana Hanzawa, 大志田 達也, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT-3のオルニチンアミノトランスフェラーゼの機能解析と構造解析,
2019年度日本ビタミン会大会, Jun. 2019.
5. Ryushi Kawakami :
超好熱アーキアのFAD依存性脱水素酵素複合体及びPLP依存性アミノ酸ラセマーゼの研究,
2019年度日本ビタミン会大会 奨励賞受賞講演, Jun. 2019.
6. Ryushi Kawakami, Kaori Kanemaru and Kumio Yokoigawa :
食品微生物や発酵食品に含まれるD-アミノ酸検出のためのUHPLC検出系の構築,
第38回日本日本食品微生物学会学術総会, Oct. 2017.
7. Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru :
Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin,
第38回日本日本食品微生物学会学術総会, Oct. 2017.
8. Ryushi Kawakami, Taku Fujiwara, Risa Kanamo and Nanako Tsuji :
超好熱アーキアPyrococcus horikoshii OT-3のアミノ酸ラセマーゼホモログの酵素化学的解析,
日本農芸化学会中四国支部第48回講演会, Jun. 2017.
9. 大志田 達也, Junji Hayashi, 里村 武範, Ryushi Kawakami, 大島 敏久 and 櫻庭 春彦 :
高度好塩菌 Haloarcula japonica 由来 2-デオキシリボース 5-リン酸アルドラーゼに関する研究,
日本農芸化学会2017年度大会, Mar. 2017.
10. Kaori Kanemaru, 生杉 笙, Ryushi Kawakami, Yasuo Oyama and Kumio Yokoigawa :
徳島県産食用植物のガン細胞増殖抑制,
日本農芸化学会2017年度(平成29年度)大会講演要旨集, Mar. 2017.
11. Ryushi Kawakami, Taku Fujiwara, Risa Kanamo and Nanako Tsuji :
超好熱アーキアPyrococcus horikoshii OT-3のD-アミノ酸培養とアミノ酸ラセマーゼ活性の相関,
2016年度極限環境生物学会, Nov. 2016.
12. 大志田 達也, Junji Hayashi, 里村 武範, Ryushi Kawakami, 大島 敏久 and 櫻庭 春彦 :
高度好塩菌 Haloarcula japonica由来2-デオキシリボース-5-リン酸アルドラーゼに関する研究,
日本農芸化学会2016年度中四国支部大会, Sep. 2016.
13. Ryushi Kawakami, Haruhiko Sakuraba, 大森 勇門 and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT3のアミノ酸ラセマーゼの酵素化学的解析,
日本農芸化学会中四国支部第45回講演会, Jun. 2016.
14. Ryushi Kawakami :
超好熱アーキアのD-アミノ酸利用とアミノ酸ラセマーゼの発見,
日本農芸化学会中四国支部第22回若手シンポジウム, Apr. 2016.
15. Ryushi Kawakami :
超好熱アーキアのL-プロリン脱水素酵素:機能・構造・応用,
日本農芸化学会中四国支部第18回若手シンポジウム, Sep. 2014.
16. Ryushi Kawakami, 山下 久美, 大森 勇門, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT-3 由来アミノ酸ラセマーゼの同定,
2014年度日本ビタミン会大会, Jun. 2014.
17. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
L-プロリン脱水素酵素からのオキシダーゼ活性除去の検討,
2013年度日本生物工学会大会, Sep. 2013.
18. 黒田 早織, Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshiiのNAD合成酵素(NADS)の機能解析,
日本農芸化学会2007年度大会, Mar. 2007.
19. Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
低温菌由来の巨大グルタミン酸脱水素酵素,
酵素工学研究会, Oct. 2006.
20. Toshihisa Ohshima, 里村 武範, Ryushi Kawakami, Haruhiko Sakuraba, 谷 裕児, Tomoki Yabutani and Junko Motonaka :
超好熱アーキアPyrobaculum islandicumの色素依存性D-プロリン脱水素酵素の機能解析と応用,
第2回D-アミノ酸研究学術講演会, Sep. 2006.
21. Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
低温菌Janthinobacterium lividum UTB1302が生産するNAD依存性グルタミン酸脱水素酵素の遺伝子クローニング,
日本ビタミン学会第58回大会, May 2006.
22. 吉行 貴司, 伊藤 佳洋, Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアSulfolobus tokodaii strain7 由来2-keto-3-deoxygluconate (KDG) aldolase の機能解析,
日本農芸化学会2006年度大会, Mar. 2006.
23. 寺井 利光, 広瀬 由鷹, 末信 一郎, 堀 照夫, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
耐熱性L-プロリン脱水素酵素の電極上への分子配向制御固定,
日本農芸化学会2006年度大会, Mar. 2006.
24. Ryushi Kawakami, Haruhiko Sakuraba, Hideaki Tsuge, 勝沼 信彦 and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshiiの新規色素依存性L-プロリン脱水素酵素の機能と構造,
日本Archaea研究会第18回講演会, Jul. 2005.
25. Ryushi Kawakami, 田頭 祐哉, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアArchaeoglobus fulgidusのNAD生合成に機能すると予想されるL-AspDHホモログの機能解析,
日本ビタミン学会第57回大会, May 2005.
26. Toshihisa Ohshima, 本多 恭子, Ryushi Kawakami, Haruhiko Sakuraba and 新居 修 :
スクモ藍建て工程に関与するインジゴ還元酵素の精製と性質,
日本農芸化学会2005年度大会, Mar. 2005.
27. Haruhiko Sakuraba, Ryushi Kawakami, Hideaki Tsuge, Shuichiro Goda, 勝沼 信彦, 安藝 謙嗣 and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshiiに存在する新規ATP含有アミノ酸脱水素酵素,
日本農芸化学会2005年度大会, Mar. 2005.
28. 廣瀬 由鷹, 木村 友和, 鄭 海涛, 末 信一朗, 堀 照夫, 片山 秀夫, Ryushi Kawakami and Toshihisa Ohshima :
リコンビナント耐熱性L-プロリン脱水素酵素を用いたLegionella属菌のDNA検出システム,
日本農芸化学会2005年度大会, Mar. 2005.
29. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, 吾郷 日出夫, 宮野 雅司, 安藝 謙嗣, 勝沼 信彦 and Toshihisa Ohshima :
P. horikoshii由来L-プロリン脱水素酵素のX線結晶構造解析,
日本生物物理学会第42回年会, Dec. 2004.
30. Toshihisa Ohshima, Ryushi Kawakami, Haruhiko Sakuraba and Hideaki Tsuge :
超好熱アーキアの色素依存性脱水素酵素の探索とその応用,
第27回日本分子生物学会年会, 369, Dec. 2004.
31. 廣瀬 由鷹, 木村 友和, 鄭 海涛, 末 信一朗, 堀 照夫, 片山 秀夫, Ryushi Kawakami and Toshihisa Ohshima :
超好熱アーキア由来色素依存性L-プロリン脱水素酵素を用いたLegionella属菌検出DNAセンサーの構築,
日本生物工学会平成16年度大会, 240, Sep. 2004.
32. Haitao Zheng, Yukata Hirose, Tomokazu Kimura, Shin-ichiro Suye, Teruo Hori, Hideo Katayama, Ryushi Kawakami and Toshihisa Ohshima :
Multilayer assembly of dye-linked L-proline dehydrogenase and poly(allylamine)ferrocene by layer-by layer adsorption method,
日本生物工学会平成16年度大会, 240, Sep. 2004.
33. Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
低温菌Janthinobacterium lividum UTB1302由来のグルタミン酸脱水素酵素の特徴,
日本生物工学会平成16年度大会, 109, Sep. 2004.
34. Ryushi Kawakami, Hideaki Tsuge, Shuichiro Goda, Haruhiko Sakuraba, 勝沼 信彦, 安藝 謙嗣 and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT-3から発見されたフラビン酵素,色素依存性L-プロリン脱水素酵素の機能と構造解析,
日本ビタミン学会第56回大会, Vol.78, No.4, 253, May 2004.
35. Haruhiko Sakuraba, Ryushi Kawakami and Toshihisa Ohshima :
超好熱アーキアThermococcus litoralisに存在する新規アラニン:グリオキシル酸アミノトランスフェラーゼ,
日本ビタミン学会第56回大会, Vol.78, No.4, 246, May 2004.
36. 本多 恭子, Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba, 新居 修 and Toshihisa Ohshima :
藍染め染色液中の好アルカリ性菌の分離とインジゴ還元酵素の諸性質の解明,
日本農芸化学会2004年度大会, 263, Mar. 2004.
37. Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba, Hideaki Tsuge and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT-3の色素依存性L-プロリン脱水素酵素の性質と構造,
日本農芸化学会2004年度大会, 118, Mar. 2004.
38. Shuichiro Goda, Koji Ono, Ryushi Kawakami, Haruhiko Sakuraba, Yutaka Kawarabayasi and Toshihisa Ohshima :
Pyrococcus horikoshii agmatinase: gene expression in E. coli and characterization,
第76回日本生化学会大会, Vol.75, No.8, 1066, Oct. 2003.
39. Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT-3に存在する2種類の色素依存性L-プロリン脱水素酵素の特徴,
日本生物工学会平成15年度大会, 45, Sep. 2003.
40. Shuichiro Goda, Haruhiko Sakuraba, Mohammad W Bhuiya, 里村 武範, Ryushi Kawakami, 倉光 成紀, 柊 弓絃 and Toshihisa Ohshima :
大腸菌によって発現された低活性超好熱菌由来グルタミン酸脱水素酵素の熱及び尿素による活性化過程のX線小角散乱と熱量計による解析,
日本アーキア研究会第16回講演会, 26, Jun. 2003.
41. Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアThermococcus profundusの色素依存性L-プロリン/NADH脱水素酵素複合体の解析,
日本農芸化学会2003年度大会, 258, Apr. 2003.
42. Shuichiro Goda, Haruhiko Sakuraba, Mohammad W Bhuiya, 里村 武範, Ryushi Kawakami, 柊 弓絃 and Toshihisa Ohshima :
好熱菌由来グルタミン酸脱水素酵素の熱及び尿素による成熟化課程の物理化学的解明,
日本農芸化学会2003年度大会, 241, Apr. 2003.
43. 里村 武範, Ryushi Kawakami, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアD-アミノ酸脱水素酵素の性質,
第3回極限環境微生物学会年会, 5-6, Dec. 2002.
44. 里村 武範, Ryushi Kawakami, 藤崎 礼子, 中山 奈央子, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
好熱好酸性アーキアSulfolobus tokodaii strain 7 由来 D- 乳酸脱水素酵素の特徴,
日本生物工学会平成14年度大会, Oct. 2002.
45. Ryushi Kawakami, 里村 武範, Shuichiro Goda, Haruhiko Sakuraba and Toshihisa Ohshima :
超好熱アーキアPyrococcus horikoshii OT3 の 色素依存性 L- プロリン脱水素酵素の精製と酵素遺伝子の同定,
日本生物工学会平成14年度大会, Oct. 2002.
46. Toshihisa Ohshima, Ryushi Kawakami and Haruhiko Sakuraba :
超好熱アーキアの色素依存性L-プロリン脱水素酵素の特徴,
第388回ビタミンB研究委員会, Aug. 2002.

Et cetera, Workshop:

1. Toshihisa Ohshima, Haruhiko Sakuraba, Ryushi Kawakami and Hideaki Tsuge :
超好熱菌のアミノ酸代謝関連酵素,NAD生合成系酵素の立体構造解析,
タンパク3000プロジェクト代謝系評価会, Nov. 2006.

Patent:

1. Toshihisa Ohshima, Haruhiko Sakuraba, Ryushi Kawakami, 吉田 洋一 and 下家 郁子 : 耐熱性2-デオキシリボース-5-リン酸アルドラーゼ, 2002-049172 (Feb. 2002), 2003-250553 (Sep. 2003), .

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

  • 遺伝子発現とアミノ酸動態の解析によるD-アミノ酸誘導性タンパク質発現の解明 (Project/Area Number: 20K05729 )
  • Functional and structural analyses of the archaeal L-proline dehydrogenase complexes (Project/Area Number: 21780099 )
  • Search by Researcher Number (90380120)