Tokushima University / Educator and Researcher Directory --- Sakamoto, Takaiku

Personal Web Page

https://researchmap.jp/takaiku-sakamoto (researchmap)

Field of Study

○	Applied Microbiology, Molecular Biology

Subject of Study

○	バイオマス資源を利用した機能性物質の微生物発酵生産

Book / Paper

Book:

1.	安藤晃規, 奥田知生, 菊川寛史, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : 油糧微生物による種々のω3脂肪酸含有油脂の発酵生産, CMC Publishing Co.,Ltd., Apr. 2021.
2.	Eiji Sakuradani and Takaiku Sakamoto : 廃グリセロールを利用した油脂発酵生産, 三恵社, Nov. 2016.
3.	Takaiku Sakamoto : 化石燃料とキノコ酵素のややこしい関係, The Society for Biotechnology, Japan, 2015. (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1543105995102575232 (CiNii: 1543105995102575232)

Academic Paper (Judged Full Paper):

1.	Hiroshi Kikukawa, Akinori Ando, Asuka Hannya, Mohd Farida Fazli Asras, Tomoyo Okuda, Takaiku Sakamoto, Kiyotaka Y. Hara, Eiji Sakuradani and Jun Ogawa : Mead acid production by disruption of Δ12-desaturase gene in Mortierella alpina 1S-4, Journal of Bioscience and Bioengineering, 2023. (Summary) Mead acid (MA; 20:3ω9) is one of the ω9 series of polyunsaturated fatty acids (PUFAs). MA is used to inhibit the inflammation of joints and is applied to the medicinal or health food field. We aimed to construct MA-producing strains with disruption of the Δ12-desaturase gene (Δ12ds) via an efficient gene-targeting system using the lig4-disrupted strain of Mortierella alpina 1S-4 as the host. The transformants showed a unique fatty acid composition that only comprised ω9-PUFAs and saturated fatty acids, while ω6-and ω3-PUFAs were not detected, and the total composition of ω9-PUFAs, including oleic acid (18:1ω9), 18:2ω9, 20:1ω9, 20:2ω9, and MA, was up to 68.4% of the total fatty acids. The MA production in the Δ12ds-disruptant reached 0.10 g/L (8.5%), which exceeded 0.050 g/L (4.6%) in the conventional Δ12ds-defective mutant JT-180. (Keyword) Mortierella alpina 1S-4 / Gene disruption / Δ12-desaturase / Polyunsaturated fatty acid / Mead acid (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jbiosc.2023.08.001 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37635046 ● Summary page in Scopus @ Elsevier: 2-s2.0-85169049369 (DOI: 10.1016/j.jbiosc.2023.08.001, PubMed: 37635046, Elsevier: Scopus)
2.	Risa Sasaki, Shogo Toda, Takaiku Sakamoto, Eiji Sakuradani and Shinsuke Shigeto : Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in Single Aurantiochytrium limacinum Cells with Linear and Nonlinear Raman Microspectroscopy, The Journal of Physical Chemistry B, Vol.127, No.12, 2708-2718, 2023. (Summary) Thraustochytrids are heterotrophic marine protists known for their high production capacity of various compounds with health benefits, such as polyunsaturated fatty acids and carotenoids. Although much effort has been focused on developing optimal cultivation methods for efficient microbial production, these high-value compounds and their interrelationships are not well understood at the single-cell level. Here we used spontaneous (linear) Raman and multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy to visualize and characterize lipids (e.g., docosahexaenoic acid) and carotenoids (e.g., astaxanthin) accumulated in single living Aurantiochytrium limacinum cells. Spontaneous Raman imaging with the help of multivariate curve resolution alternating least-squares enabled us to make unambiguous assignments of the molecular components we detected and derive their intracellular distributions separately. Near-IR excited CARS imaging yielded the Raman images at least an order of magnitude faster than spontaneous Raman imaging, with suppressed contributions of carotenoids. As the culture time increased from 2 to 5 days, the lipid amount increased by a factor of 7, whereas the carotenoid amount did not change significantly. Furthermore, we observed a highly localized component in A. limacinum cells. This component was found to be mixed crystals of guanine and other purine derivatives. The present study demonstrates the potential of the linear nonlinear Raman hybrid approach that allows for accurate molecular identification and fast imaging in a label-free manner to link information derived from single cells with strategies for mass culture of useful thraustochytrids. (Keyword) Guanine / Hydrocarbons / Imaging / Lipids / Raman spectroscopy (Tokushima University Institutional Repository) ● Metadata: 118912 (Link to Publication Site) ● Publication site (DOI): 10.1021/acs.jpcb.3c00302 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36920390 ● Search Scopus @ Elsevier (PMID): 36920390 ● Search Scopus @ Elsevier (DOI): 10.1021/acs.jpcb.3c00302 (Tokushima University Institutional Repository: 118912, DOI: 10.1021/acs.jpcb.3c00302, PubMed: 36920390)
3.	Takaiku Sakamoto, Yusuke Ikeda, Naruho Masuda and Eiji Sakuradani : Ethanol Enhances Astaxanthin Production by Aurantiochytrium sp. O5-1-1, Journal of Oleo Science, Vol.72, No.4, 441-446, 2023. (Summary) Two-percent ethanol increased the astaxanthin productivity of heterotrophic microalgae Aurantiochytrium sp. O5-1-1 to 2.231 mg/L, 45-fold higher than under ethanol-free condition. Ethanol in the medium decreased at the same rate as spontaneous volatilization, suggesting that it was not a transient signaling factor but a continuous stress on the cells. The triply mutated strain OM3-3 produced 5.075 mg/L astaxanthin under 2% ethanol conditions. Furthermore, the astaxanthin accumulation of the mutant OM3-9 was 0.895 mg/g, which was 150-fold higher than that of strain O5-1-1 in ethanol-free condition. These results are beneficial for the commercial exploitation of carotenoids producing Aurantiochytrium spp. (Keyword) Aurantiochytrium / astaxanthin / ethanol stress / β-carotene (Tokushima University Institutional Repository) ● Metadata: 118143 (Link to Publication Site) ● Publication site (DOI): 10.5650/jos.ess22308 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36990751 ● Search Scopus @ Elsevier (PMID): 36990751 ● Search Scopus @ Elsevier (DOI): 10.5650/jos.ess22308 (Tokushima University Institutional Repository: 118143, DOI: 10.5650/jos.ess22308, PubMed: 36990751)
4.	Kasumi Nakagawa, Michiki Takeuchi, Manami Tada, Momoka Matsunaga, Masami Kugo, Suzuna Kiyofuji, Mayu Kikuchi, Kazuya Yomota, Takaiku Sakamoto, Kenji Kano, Jun Ogawa and Eiji Sakuradani : Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions, Bioscience, Biotechnology, and Biochemistry, Vol.86, No.2, 273-281, 2022. (Summary) In natural indigo dyeing, the water-insoluble indigo included in the composted indigo leaves called sukumo is converted to water-soluble leuco-indigo through the reduction activities of microorganisms under alkaline conditions. To understand the relationship between indigo reduction and microorganisms in indigo-fermentation suspensions, we isolated and identified the microorganisms that reduce indigo and analyzed the microbiota in indigo-fermentation suspensions. Indigo-reducing microorganisms, which were not isolated by means of a conventional indigo carmine-reduction assay method, were isolated by using indigo as a direct substrate and further identified and characterized. We succeeded in isolating bacteria closely related to Corynebacterium glutamicum, Chryseomicrobium aureum, Enterococcus sp. for the first time. Anthraquinone was found to be an effective mediator that facilitated the indigo-reduction activity of the isolated strains. On analysis of the microbiota in indigo-fermentation suspensions, the ratio of indigo-reducing bacteria and others was found to be important for maintaining the indigo-reduction activity. (Keyword) Indigo / Microbiota / fermentation (Link to Publication Site) ● Publication site (DOI): 10.1093/bbb/zbab209 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1093/bbb/zbab209 (DOI: 10.1093/bbb/zbab209)
5.	Naomi Murakawa, Takaiku Sakamoto, Mizuho Kanoh, Park Si-Bum, Shigenobu Kishino, Jun Ogawa and Eiji Sakuradani : Microbial production of hydroxy fatty acids utilizing crude glycerol, Biocatalysis and Agricultural Biotechnology, Vol.39, 102286, 2022. (Keyword) Fusarium solani / 10-Hydroxyoctadecanoic acid / 10-Hydroxy-cis-12-octadecenoic acid / 10-Oxo-octadecanoic acid / Fatty acid hydration / Crude glycerol (Tokushima University Institutional Repository) ● Metadata: 117466 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bcab.2022.102286 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1360013168859284992 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bcab.2022.102286 (Tokushima University Institutional Repository: 117466, DOI: 10.1016/j.bcab.2022.102286, CiNii: 1360013168859284992)
6.	Takaiku Sakamoto, Yuichi Kamegawa, Chinami Kurita, Mizuho Kanoh, Naomi Murakawa and Eiji Sakuradani : Efficient production of biolipids by crude glycerol-assimilating fungi, Bioresource Technology Reports, Vol.16, 100861, 2021. (Summary) The aim of this study was to isolate microorganisms utilizing crude glycerol as a carbon source efficiently and to evaluate their lipid productivity. Fusarium oxysporum W1 grew well on medium containing 20% crude glycerol as well as 50% pure glycerol. The dry cell weights and total fatty acids of F. oxysporum W1 reached 24.5 g/L and 12.4 g/L. Penicillium sp. N1 and P. citrinum N3 were found to accumulate free fatty acids to as much as 56.2% and 48.5% of total fatty acids, respectively, on cultivation in the crude glycerol-containing medium. These strains grew well on medium containing crude glycerol only heat-treated at 80 105 °C without autoclave sterilization. (Keyword) Crude glycerol / Biolipid / Penicillium / Fusarium (Tokushima University Institutional Repository) ● Metadata: 117465 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.biteb.2021.100861 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.biteb.2021.100861 (Tokushima University Institutional Repository: 117465, DOI: 10.1016/j.biteb.2021.100861)
7.	Kasumi Nakagawa, Michiki Takeuchi, Mayu Kikuchi, Manami Tada, Takaiku Sakamoto, Kenji Kano, Jun Ogawa and Eiji Sakuradani : Voltammetric in-situ monitoring of leuco-indigo in indigo-fermenting suspensions, Journal of Bioscience and Bioengineering, Vol.131, No.5, 565-571, 2021. (Summary) Cyclic voltammetry was successfully applied to in-vivo monitoring of leuco-indigo in indigo-fermenting suspensions under quiescent conditions without deoxygenation; the working and counter electrodes were kept on the surface of each suspension by a polyethylene vinyl alcohol tube holder. The anodic peak current was used as a measure of the leuco-indigo concentration. The voltammetric wave shape suggested partial solubilization of the indigo with some macromolecules in the fermenting suspensions, which lead to an in-situ method without any electrode surface pretreatment. The anodic peak current well reflected the dyeing activity of a suspensions. The results obtained for laboratory-level fermentation systems clarified the number of days required for dye fermentation, the effectiveness of addition of old suspension as an additive for preparing fresh fermenting suspensions, and the importance of addition of a nitrogen-based nutrient as well as a glucose-based one to recover the indigo-reducing activity. The method can also be applied to determine the amounts of indigo in used dye suspensions and extracts of fermented indigo leaves (sukumo) by adding a chemical reduction pretreatment. (Keyword) Indigo / fermentation / electrochemistry / Anaerobic condition / Cyclic voltammetry (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jbiosc.2021.01.005 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbiosc.2021.01.005 (DOI: 10.1016/j.jbiosc.2021.01.005)
8.	Brian KH Mo, Akinori Ando, Ryohei Nakatsuji, Tomoyo Okuda, Yuki Takemoto, Hiroyuki Ikemoto, Hiroshi Kikukawa, Takaiku Sakamoto, Eiji Sakuradani and Jun Ogawa : Characterization of ω3 fatty acid desaturases from oomycetes and their application toward eicosapentaenoic acid production in Mortierella alpina, Bioscience, Biotechnology, and Biochemistry, Vol.85, No.5, 1252-1265, 2021. (Summary) ω3 polyunsaturated fatty acids are currently obtained mainly from fisheries, thus sustainable alternative sources such as oleaginous microorganisms are required. Here we describe the isolation, characterization, and application of three novel ω3 desaturases with ω3 polyunsaturated fatty acid-producing activity at ordinary temperatures (28 °C). First, we selected Pythium sulcatum and Plectospira myriandra after screening for oomycetes with high eicosapentaenoic acid/arachidonic acid ratios and isolated the genes psulω3 and pmd17, respectively, which encode ω3 desaturases. Subsequent characterization showed that PSULω3 exhibited ω3 desaturase activity on both C18 and C20 ω6 polyunsaturated fatty acids while PMD17 exhibited ω3 desaturase activity exclusively on C20 ω6 polyunsaturated fatty acids. Expression of psulω3 and pmd17 in the arachidonic acid-producer Mortierella alpina resulted in transformants that produced eicosapentaenoic acid/total fatty acid values of 38% and 40%, respectively, at ordinary temperatures. These ω3 desaturases should facilitate the construction of sustainable ω3 polyunsaturated fatty acid sources. (Keyword) Eicosapentaenoic acid / Pythium sulcatum / Plectospira myriandra / ω3 desaturase / Mortierella alpina (Link to Publication Site) ● Publication site (DOI): 10.1093/bbb/zbaa123 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1093/bbb/zbaa123 (DOI: 10.1093/bbb/zbaa123)
9.	Kasumi Nakagawa, Michiki Takeuchi, Mayu Kikuchi, Suzuna Kiyofuji, Masami Kugo, Takaiku Sakamoto, Kenji Kano, Jun Ogawa and Eiji Sakuradani : Mechanistic Insights into Indigo Reduction in Indigo Fermentation: A Voltammetric Study, Electrochemistry, Vol.89, 25-30, 2021. (Summary) Indigo is one of the oldest natural blue dyes. Microorganisms and their enzymatic activities are deeply involved in the traditional indigo staining procedure. To elucidate the mechanism of the microbial indigo reduction, we directly performed cyclic voltammetry on alkaline fermenting dye suspensions. A pair of characteristic redox peaks of leuco-indigo was observed in a supernatant fluid of the fermenting dye suspension. On the other hand, it was found that the indigo/leuco-indigo redox couple mediated two-way microbially catalyzed oxidation and reduction in a sediment-rich suspension of the fermenting suspension. Acetaldehyde was supposed to be the electron donor and acceptor of the catalytic reactions. In order to verify the bioelectrocatalytic reaction, we isolated indigo-reducing bacterium K2-3 from the fermenting suspension, and the two-way bioelectrocatalysis was successfully restaged in a model system containing K2-3 and methyl viologen (as a soluble mediator instead of indigo) as well as acetaldehyde at pH 10. (Keyword) Indigo Reduction Mechanism / Acetaldehyde / Bioelectrocatalysis / Mediator (Tokushima University Institutional Repository) ● Metadata: 116295 (Link to Publication Site) ● Publication site (DOI): 10.5796/electrochemistry.20-00123 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1390568456342112896 ● Summary page in Scopus @ Elsevier: 2-s2.0-85099770189 (Tokushima University Institutional Repository: 116295, DOI: 10.5796/electrochemistry.20-00123, CiNii: 1390568456342112896, Elsevier: Scopus)
10.	Takeru Koga, Takaiku Sakamoto, Eiji Sakuradani and Akihiro Tai : Neurite Outgrowth-Promoting Activity of Compounds in PC12 Cells from Sunflower Seeds, Molecules, Vol.25, No.20, 4748, 2020. (Summary) In the current super-aging society, the establishment of methods for prevention and treatment of Alzheimer's disease (AD) is an urgent task. One of the causes of AD is thought to be a decrease in the revel of nerve growth factor (NGF) in the brain. Compounds showing NGF-mimicking activity and NGF-enhancing activity have been examined as possible agents for improving symptoms. In the present study, sunflower seed extract was found to have neurite outgrowth-promoting activity, which is an NGF-enhancing activity, in PC12 cells. To investigate neurite outgrowth-promoting compounds from sunflower seed extract, bioassay-guided purification was carried out. The purified active fraction was obtained by liquid-liquid partition followed by some column chromatographies. Proton nuclear magnetic resonance and gas chromatography-mass spectrometry analyses of the purified active fraction indicated that the fraction was a mixture of β-sitosterol, stigmasterol and campesterol, with β-sitosterol being the main component. Neurite outgrowth-promoting activities of β-sitosterol, stigmasterol, campesterol and cholesterol were evaluated in PC12 cells. β-Sitosterol and stigmasterol showed the strongest activity of the four sterol compounds (β-sitosterol stigmasterol > campesterol > cholesterol), and cholesterol did not show any activity. The results indicated that β-sitosterol was the major component responsible for the neurite outgrowth-promoting activity of sunflower seeds. Results of immunostaining also showed that promotion by β-sitosterol of neurite formation induced by NGF was accompanied by neurofilament expression. β-Sitosterol, which showed NGF-enhancing activity, might be a candidate ingredient in food for prevention of AD. (Keyword) Alzheimer's disease / sunflower seed / NGF / β-sitosterol / neurite outgrowth-promoting activit (Tokushima University Institutional Repository) ● Metadata: 116242 (Link to Publication Site) ● Publication site (DOI): 10.3390/molecules25204748 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.3390/molecules25204748 (Tokushima University Institutional Repository: 116242, DOI: 10.3390/molecules25204748)
11.	Takaiku Sakamoto, Eiji Sakuradani, Tomoyo Okuda, Hiroshi Kikukawa, Akinori Ando, Shigenobu Kishino, Yoshihiro Izumi, Takeshi Bamba, Jun Shima and Jun Ogawa : Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids, Bioresource Technology, Vol.245, 1610-1615, 2017. (Summary) The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain. (Tokushima University Institutional Repository) ● Metadata: 113896 (Link to Publication Site) ● Publication site (DOI): 10.1016/j.biortech.2017.06.089 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28673516 ● Search Scopus @ Elsevier (PMID): 28673516 ● Search Scopus @ Elsevier (DOI): 10.1016/j.biortech.2017.06.089 (Tokushima University Institutional Repository: 113896, DOI: 10.1016/j.biortech.2017.06.089, PubMed: 28673516)
12.	Tomohiro Fujita, Hiroshi Kawashima, Eiji Sakuradani, Takaiku Sakamoto, Akinori Ando, Jun Ogawa and Sakayu Shimizu : Essential fatty acids for oleaginous fungus Mortierella alpina, Biocatalysis and Agricultural Biotechnology, Vol.8, 167-170, 2016. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.bcab.2016.08.011 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1016/j.bcab.2016.08.011 (DOI: 10.1016/j.bcab.2016.08.011)
13.	Tomoyo Okuda, Akinori Ando, Hiroaki Negoro, Tatsuya Muratsubaki, Hiroshi Kikukawa, Takaiku Sakamoto, Eiji Sakuradani, Sakayu Shimizu and Jun Ogawa : Eicosapentaenoic acid (EPA) production by an oleaginous fungus Mortierella alpina expressing heterologous the Δ17 desaturase gene under ordinary temperature, European Journal of Lipid Science and Technology : EJLST, Vol.117, No.12, 1919-1927, 2015. (Summary) The oleaginous fungus Mortierella alpina is known to accumulate eicosapentaenoic acid (EPA) only when cultivated at a low temperature (below 15°C). Here, we investigated EPA production at a ordinary temperature (28°C) by expressing the Saprolegnia diclina 17 desaturase gene (sdd17m) in M. alpina ST1358, an 3-desaturation activity-defective mutant derived from M. alpina 1S-4. Expression of the exogenous gene was confirmed by EPA accumulation in transformants at both 28°C and 12°C. The EPA content in total lipids produced by transformants was over 20% at 28°C. Bench-scale fermentation with a 5-L jar fermentor showed that EPA content reached 26.4% of total fatty acids, and final EPA production reached 1.8 g/L. This is the first study to report the accumulation of EPA in M. alpina at a ordinary temperature, and provide a platform technology for the industrial production of EPA using M. alpina as a promising source for EPA. (Keyword) 3 polyunsaturated fatty acid / ordinary temperature / Mortierella alpina / Saprolegnia diclina (Link to Publication Site) ● Publication site (DOI): 10.1002/ejlt.201400657 (Link to Search Site for Scientific Articles) ● Search Scopus @ Elsevier (DOI): 10.1002/ejlt.201400657 (DOI: 10.1002/ejlt.201400657)
14.	Takashi Suetomi, Takaiku Sakamoto, Yoshitaka Tokunaga, Toru Kameyama, Yoichi Honda, Hisatoshi Kamitsuji, Isamu Kameshita, Kousuke Izumitsu, Kazumi Suzuki and Toshikazu Irie : Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus., Current Genetics, Vol.61, No.2, 127-140, 2015. (Summary) Previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (LiP) and manganese peroxidase (MnP) using a calmodulin (CaM) inhibitor, W7, in the white-rot fungus Phanerochaete chrysosporium; this suggested that CaM positively regulates their expression. Here, we studied the role of CaM in another white-rot fungus, Pleurotus ostreatus, which produces MnP and versatile peroxidase (VP), but not LiP. W7 upregulated Mn(2+)-dependent oxidation of guaiacol, suggesting that CaM negatively regulates the production of the enzymes. Suppression of CaM in P. ostreatus using RNAi also led to upregulation of enzyme activity, whereas overexpression of CaM in P. ostreatus caused downregulation. Real-time RT-PCR showed that MnP1-6 and VP3 levels in the CaM-knockdown strain were higher than those in the wild-type strain, while MnP-5 and -6 and VP1 and 2 levels in the CaM-overexpressing strain were lower than in the wild type. Moreover, we also found that another ligninolytic enzyme, laccase, which is not produced by P. chrysosporium, was negatively regulated by CaM in P. ostreatus similar to MnP and VP. Although overexpression of CaM did not reduce the ability of P. ostreatus to digest beech wood powder, the percentage of lignin remaining in the digest was slightly higher than in the wild-type strain digest. (Keyword) Lignin / Basidiomycetes / Manganese peroxidase / Calmodulin (Link to Publication Site) ● Publication site (DOI): 10.1007/s00294-014-0460-z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25407463 ● Search Scopus @ Elsevier (PMID): 25407463 ● Search Scopus @ Elsevier (DOI): 10.1007/s00294-014-0460-z (DOI: 10.1007/s00294-014-0460-z, PubMed: 25407463)
15.	Tomoyo Okuda, Akinori Ando, Hiroaki Negoro, Hiroshi Kikukawa, Takaiku Sakamoto, Eiji Sakuradani, Sakayu Shimizu and Jun Ogawa : Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S-4., Journal of Bioscience and Bioengineering, Vol.120, No.3, 299-304, 2015. (Summary) We investigated the omega-3 eicosatetraenoic acid (ETA) production by molecular breeding of the oleaginous fungus Mortierella alpina, which can slightly accumulate ETA only when cultivated at a low temperature. The endogenous ω3-desaturase gene or the heterologous Saprolegnia diclina Δ17 (sdd17m) desaturase gene were overexpressed in M. alpina S14, a Δ5-desaturation activity-defective mutant derived from M. alpina 1S-4. M. alpina S14 transformants introduced with the endogenous ω3-desaturase gene showed ETA at 42.1% content in the total lipids that was 84.2-fold and 3.2-fold higher than that of the wild-type strain 1S-4 and host strain S14, respectively, when cultivated at 12°C. No accumulation of ETA was observed at 28°C. In contrast, transformants with the heterologous sdd17m gene showed 24.9% of the content of total lipids at 28°C. These results indicated that these M. alpina S14 transformants are promising strains for the production of ETA, which is hard to obtain from natural sources. (Link to Publication Site) ● Publication site (DOI): 10.1016/j.jbiosc.2015.01.014 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25845716 ● Summary page in Scopus @ Elsevier: 2-s2.0-84938292293 (DOI: 10.1016/j.jbiosc.2015.01.014, PubMed: 25845716, Elsevier: Scopus)
16.	Hiroshi Kikukawa, Eiji Sakuradani, Masato Nakatani, Akinori Ando, Tomoyo Okuda, Takaiku Sakamoto, Misa Ochiai, Sakayu Shimizu and Jun Ogawa : Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid., Current Genetics, Vol.61, No.4, 579-589, 2015. (Summary) To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The ku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo--linolenic acid (DGLA)-producing strains were constructed by disruption of the 5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the ku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the 5-desaturase gene was succeeded using the ku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4. (Link to Publication Site) ● Publication site (DOI): 10.1007/s00294-015-0481-2 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25782448 ● Summary page in Scopus @ Elsevier: 2-s2.0-84944513241 (DOI: 10.1007/s00294-015-0481-2, PubMed: 25782448, Elsevier: Scopus)
17.	Yuki Yao, Takaiku Sakamoto, Yoichi Honda, Yasuyuki Kagotani, Kousuke Izumitsu, Kazumi Suzuki and Toshikazu Irie : The white-rot fungus pleurotus ostreatus transformant overproduced intracellular cAMP and laccase., Bioscience, Biotechnology, and Biochemistry, Vol.77, No.11, 2309-2311, 2013. (Summary) Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid. (Keyword) Copper Sulfate / Coumaric Acids / Culture Media / Cyclic AMP / Fungal Proteins / GTP-Binding Protein alpha Subunits / Gene Expression Regulation, Fungal / Laccase / Mutation / Pleurotus / Transformation, Genetic / Transgenes (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.130470 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24200784 ● Search Scopus @ Elsevier (PMID): 24200784 ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.130470 (DOI: 10.1271/bbb.130470, PubMed: 24200784)
18.	Takaiku Sakamoto, Yoichi Honda, Isamu Kameshita, Kazumi Suzuki and Toshikazu Irie : Isolation and heterologous expression of the Phanerochaete chrysosporium calmodulin gene, Mycoscience, Vol.54, No.3, 241-246, 2013. (Keyword) Basidiomycete / Electrophoretic migration shift / Pull-down analysis / RACE analysis (Link to Publication Site) ● Publication site (DOI): 10.1016/j.myc.2012.09.017 (Link to Search Site for Scientific Articles) ● CiNii @ National Institute of Informatics (CRID): 1520010380492596224 ● Search Scopus @ Elsevier (DOI): 10.1016/j.myc.2012.09.017 (DOI: 10.1016/j.myc.2012.09.017, CiNii: 1520010380492596224)
19.	Takaiku Sakamoto, Yuki Yao, Yoshifumi Hida, Yoichi Honda, Takashi Watanabe, Wataru Hashigaya, Kazumi Suzuki and Toshikazu Irie : A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium., AMB Express, Vol.2, 7, 2012. (Summary) The capacity of white-rot fungi to degrade wood lignin may be highly applicable to the development of novel bioreactor systems, but the mechanisms underlying this function are not yet fully understood. Lignin peroxidase (LiP) and manganese peroxidase (MnP), which are thought to be very important for the ligninolytic property, demonstrated increased activity in Phanerochaete chrysosporium RP-78 (FGSC #9002, ATCC MYA-4764™) cultures following exposure to 5 mM cyclic adenosine 3', 5'-monophosphate (cAMP) and 500 μM 3'-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that transcription of most LiP and MnP isozyme genes was statistically significantly upregulated in the presence of the cAMP and IBMX compared to the untreated condition. However, 100 μM calmodulin (CaM) inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), which had insignificant effects on fungal growth and intracellular cAMP concentration, not only offset the increased activity and transcription induced by the drugs, but also decreased them to below basal levels. Like the isozyme genes, transcription of the CaM gene (cam) was also upregulated by cAMP and IBMX. These results suggest that cAMP signaling functions to increase the transcription of LiP and MnP through the induction of cam transcription. (Link to Publication Site) ● Publication site (DOI): 10.1186/2191-0855-2-7 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22273182 ● Search Scopus @ Elsevier (PMID): 22273182 ● Search Scopus @ Elsevier (DOI): 10.1186/2191-0855-2-7 (DOI: 10.1186/2191-0855-2-7, PubMed: 22273182)
20.	Takaiku Sakamoto, Hironori Kitaura, Masahiko Minami, Yoichi Honda, Takashi Watanabe, Akio Ueda, Kazumi Suzuki and Toshikazu Irie : Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium., Current Genetics, Vol.56, No.5, 401-410, 2010. (Summary) We investigated the effects of a calmodulin (CaM) inhibitor, W-7, on the expression of lignin peroxidase (LiP) and manganese peroxidase (MnP) genes in Phanerochaete chrysosporium to consider the role of cam gene, which was upregulated in parallel with the total activities of LiP and MnP in our previous transcriptomic analysis. The addition of 100 μM W-7 to the fungal cultures repressed the total activities of LiP and MnP, whereas the addition of 100 μM W-5, which is a control drug of W-7, retained approximately half of them, indicating that the effect of W-7 was attributable to CaM inhibition. Real-time reverse transcription polymerase chain reaction analysis revealed that most of lip and mnp isozyme genes predicted from whole-genome data were significantly inhibited by W-7 at the transcription level (P ≤ 0.05). These results suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level. (Keyword) Calcium-Calmodulin-Dependent Protein Kinases / Calmodulin / DNA Primers / Fungal Proteins / Gene Expression Regulation, Fungal / Genes, Fungal / Genome, Fungal / Lignin / Peroxidases / Phanerochaete / Polymerase Chain Reaction / signal transduction / Sulfonamides / Transcription, Genetic / Trifluoperazine (Link to Publication Site) ● Publication site (DOI): 10.1007/s00294-010-0309-z (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20532887 ● Search Scopus @ Elsevier (PMID): 20532887 ● Search Scopus @ Elsevier (DOI): 10.1007/s00294-010-0309-z (DOI: 10.1007/s00294-010-0309-z, PubMed: 20532887)
21.	Masahiko Minami, Kazumi Suzuki, Akifumi Shimizu, Tomohiro Hongo, Takaiku Sakamoto, Naoki Ohyama, Hironori Kitaura, Akiho Kusaka, Kenji Iwama and Toshikazu Irie : Changes in the gene expression of the white rot fungus Phanerochaete chrysosporium due to the addition of atropine., Bioscience, Biotechnology, and Biochemistry, Vol.73, No.8, 1722-1731, 2009. (Summary) We constructed a LongSAGE (Long Serial Analysis of Gene Expression) library from a 3-d culture of Phanerochaete chrysosporium supplemented with atropine, which inhibits the production of lignin-degrading enzymes. The library (the atropine library) contains 13,108 LongSAGE tags and 6,783 unique tags. The gene expression profile represented by the tags was compared with those of two previously constructed libraries, one of which was constructed using 2-d cultures in which the fungus had not yet produced ligninolytic enzymes (the 2-d library) and the other was constructed using 3-d cultures in which the fungus had just started to produce the enzymes (the 3-d library). We found a total of 595 genes that were at least twice more highly or at least twice less highly expressed in the 3-d library than in the 2-d library or the atropine library, and the fluctuations were statistically significant. The relationships among these 595 genes were considered using cluster analysis. Of the 595 genes, 164 showed expression patterns similar to those of four ligninolytic enzyme genes, which were more expressed on day 3 than under any other conditions. Many of these 164 genes comprised genes possibly involved in lignin degradation, lipid metabolism, xenobiotic degradation, stress response, or signal transduction pathways. (Keyword) Atropine / Base Sequence / Cluster Analysis / Culture Media / Gene Expression Regulation, Fungal / Gene Library / Genes, Fungal / Lignin / Lipid Metabolism / Phanerochaete / signal transduction / Stress, Physiological (Link to Publication Site) ● Publication site (DOI): 10.1271/bbb.80870 (Link to Search Site for Scientific Articles) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19661694 ● Search Scopus @ Elsevier (PMID): 19661694 ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.80870 (DOI: 10.1271/bbb.80870, PubMed: 19661694)

Review, Commentary:

1.	安藤晃規, 奥田知生, 菊川寛史, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : Production of Various ω3-polyunsaturated Fatty Acid-containing Lipids by Oleaginous Microorganisms, BIO INDUSTRY, Vol.37, No.10, 3-13, Oct. 2020.

Proceeding of International Conference:

1.	Eiji Sakuradani, Yoshida Kai, Murakawa Naomi and Takaiku Sakamoto : Studies on filamentous fungus Fusarium sp. accumulating hydroxy fatty acids, 2022 AOCS Annual Meeting & Expo, May 2022.
2.	Eiji Sakuradani, Naomi Murakawa, Konomi Ueno, Takaiku Sakamoto, Yuki Soma, Yoshihiro Izumi, Takeshi Bamba, Akinori Ando, Shigenobu Kishino and Jun Ogawa : Molecular breeding using a fatty acid hydratase from a filamentous fungus, The 15th International Symposium on Biocatalysis and Agricultural Biotechnology, Hiroshima, Sep. 2019.
3.	Naomi Murakawa, Takaiku Sakamoto and Eiji Sakuradani : Oxidized fatty acids produced by filamentous fungus Fusarium sp., The 1st International Symposium on Chemical Communication(ISCC2019), Tokyo, Jan. 2019.
4.	Eiji Sakuradani, Naomi Murakawa and Takaiku Sakamoto : Production of Microbial Lipids using Crude Glycerol., 2018 AOCS Annual Meeting & Expo, Minnesota USA, May 2018.
5.	Toshikazu Irie, Takashi Suetomi, Takaiku Sakamoto, Yoshitaka Tokunaga, Yoichi Honda, Hisatoshi Kamitsuji, Isamu Kameshita, Kousuk Izumitsu and Kazumi Suzuki : Effects of CaM on manganese peroxidase expression in Pleurotus ostreatus., The 10th International Mycological Congress, Bangkok Thailand, Aug. 2014.
6.	Eiji Sakuradani, Akinori Ando, Takaiku Sakamoto, Jun Shima, Sakayu Shimizu and Jun Ogawa : Microbial Production of Various Polyunsaturated Fatty Acids by Molecular Breeding of oleaginous fungus Mortierella alpina, The 1st International Symposium on Microbial Technology for Food and Energy Security, Bangkok Thailand, Nov. 2013.
7.	Takaiku Sakamoto, Yoichi Honda, Isamu Kameshita, Takashi Watanabe, Kazumi Suzuki and Toshikazu Irie : Screening of calmodulin-binding proteins expressed during ligninolytic enzyme production., IUMS2011: The Unlimited World of Microbes, Sapporo Japan, Sep. 2011.
8.	Yuki Yao, Takaiku Sakamoto, Yoichi Honda, Takashi Watanabe, Wataru Hashigaya, Yoshihumi Hida, Kazumi Suzuki and Toshikazu Irie : Effects of cAMP on ligninolytic enzymes production in Phanerochaete chrysosporium., IUMS2011: The Unlimited World of Microbes, Sapporo Japan, Sep. 2011.
9.	Toshikazu Irie, Takaiku Sakamoto, Yuki Yao and Kazumi Suzuki : Research on signaling pathways in ligninolytic enzyme expression., World Congress of Microbes-2011, Beijing China, Aug. 2011.
10.	Takaiku Sakamoto, Masahiko Minami, Yoichi Honda, Takashi Watanabe, Kazumi Suzuki and Toshikazu Irie : A calmodulin inhibitor, W-7 repressed expression of ligninolytic enzyme genes., The 9th International Mycological Congress, Edinburgh UK, Aug. 2010.
11.	Toshikazu Irie, Takaiku Sakamoto and Kazumi Suzuki : A study on signaling pathway of ligninolytic enzyme expression, The 9th International Mycological Congress, Edinburgh UK, Aug. 2010.

Proceeding of Domestic Conference:

1.	SHIBATA Yume, IBARAGI Masahiro, IKEDA Shiori, Takaiku Sakamoto and Eiji Sakuradani : フザリウム属糸状菌におけるΔ6 脂肪酸不飽和化酵素遺伝子発現によるγ-リノレン酸生産, 酵素工学研究会第92回講演会, Nov. 2024.
2.	IKEDA Shiori, IBARAGI Masahiro, SHIBATA Yume, 玉野孝一, Takaiku Sakamoto and Eiji Sakuradani : Breeding of strains overexpressing the Aspergillus oryzae-derived lipase gene in filamentous fungi of the genus Fusarium, 第23回糸状菌分子生物学コンファレンス, Nov. 2024.
3.	SHIBATA Yume, IBARAGI Masahiro, IKEDA Shiori, Takaiku Sakamoto and Eiji Sakuradani : Screening and transformation of Fusarium and closely-related species that produce high levels of hydroxy fatty acids, 第23回糸状菌分子生物学コンファレンス, Nov. 2024.
4.	IBARAGI Masahiro, SHIBATA Yume, IKEDA Shiori, Takaiku Sakamoto and Eiji Sakuradani : Heterologous expression of Δ6 desaturase from Mortierella alpina is suitable for phenotypic evaluation of Fusarium sp. transformants, 第23回糸状菌分子生物学コンファレンス, Nov. 2024.
5.	Eiji Sakuradani, SHIBATA Yume, IBARAGI Masahiro, IKEDA Shiori and Takaiku Sakamoto : 廃グリセロールや植物油を利用した Fusarium 属糸状菌による水酸化脂肪酸生産, 第76回日本生物工学会大会, Sep. 2024.
6.	Kume Izumi, Okuno Nene, Ikekita Aika, Takaiku Sakamoto and Eiji Sakuradani : Aurantiochytrium属微細藻類を用いたカロテノイド生産条件の検討, 第9回ラビリンチュラシンポジウム, Aug. 2024.
7.	OHHATA Haruka, Kasumi Nakagawa, 竹内道樹, Takaiku Sakamoto and Eiji Sakuradani : 藍染液中のインジゴ還元におけるリグニンの役割, 日本農芸化学会中四国支部第67回講演会(例会), Jan. 2024.
8.	YOSHIDA Kai, Takaiku Sakamoto, 杉森大助 and Eiji Sakuradani : フザリウム属糸状菌による様々な植物油からの水酸化脂肪酸への変換条件の検討, 日本農芸化学会中四国支部第67回講演会(例会), Jan. 2024.
9.	YOSHIDA Kai, Takaiku Sakamoto, 杉森大助 and Eiji Sakuradani : フザリウム属糸状菌による植物油からの水酸化脂肪酸への変換条件の検討, 酵素工学研究会第90回講演会, Nov. 2023.
10.	Kasumi Nakagawa, MATSUNAGA Momoka, OHHATA Haruka, Takaiku Sakamoto and Eiji Sakuradani : 微生物によるインジゴ還元を促進する物質の探索, 2023年度生物工学若手研究者の集い(若手会)夏のセミナー2023, Jun. 2023.
11.	迫野眞大, 安藤晃規, 奥田知生, モブライアン, 中辻諒平, 竹本有貴, 池本裕之, 菊川寛史, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : Mortierella alpinaによる常温EPA生産を目的とした新規ω3不飽和化酵素の探索および機能解析, 日本農芸化学会2023年度大会, Mar. 2023.
12.	SANO Sohhei, TAKASAKI Ren, 岡久修己, Takaiku Sakamoto and Eiji Sakuradani : 香気成分生産性に優れた野生酵母の探索, 日本農芸化学会2022年度中四国支部大会(第63回講演会), Sep. 2022.
13.	竹内道樹, Kasumi Nakagawa, 菊地真由, 宋和慶盛, MATSUNAGA Momoka, Takaiku Sakamoto, 安藤晃規, 小川順, 加納健司 and Eiji Sakuradani : 藍染め染色液の電気化学的解析と微生物燃料電池への応用, 電気化学会第89回大会, Mar. 2022.
14.	Naoki Masuda, Takaiku Sakamoto, 西海信, Takahito Watanabe, Eiji Sakuradani, Taro Mito and Rie Mukai : 食用昆虫の摂取が実験動物の代謝に及ぼす影響, 日本農芸化学会2022年度大会, Mar. 2022.
15.	TAKASAKI Ren, SANO Sohhei, 岡久修己, Takaiku Sakamoto and Eiji Sakuradani : Non-Saccharomyces酵母変異株の探索とそれらを利用した簡易ビール醸造試験, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
16.	KITAGUCHI Naoki, Mao Kusunoki, KINOUCHI Kanae, Takaiku Sakamoto and Eiji Sakuradani : 担子菌における子実体形成関連遺伝子の探索, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
17.	MASUDA Naruho, Yusuke Ikeda, KUME Izumi, Takaiku Sakamoto and Eiji Sakuradani : 油糧微生物ラビリンチュラ類の変異によるアスタキサンチン高生産株の育種, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
18.	HANIBUCHI Kohki, MAEDA Ryogo, Takaiku Sakamoto and Eiji Sakuradani : 微生物によるデオキシコール酸代謝機構の解析, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
19.	Yuji Nakamura, EGUCHI Kazuma, Takaiku Sakamoto and Eiji Sakuradani : 油糧微細藻Thraustochytridの変異育種による脂肪酸組成改変, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
20.	Mari Yoshizaki, Tomomi Inoue, KOHNO Rino, Takaiku Sakamoto and Eiji Sakuradani : Bacillus属細菌による抗菌活性薬剤Iturin生産に関する研究, 日本農芸化学会中四国支部第61回講演会(例会), Jan. 2022.
21.	糸川未来, 安藤晃規, 奥田知生, MO Brian King Himm, 中辻諒平, 竹本有貴, 池本裕之, 菊川寛史, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : Mortierella alpina による EPA 生産のための ω3 不飽和化酵素の探索及び機能評価, 2021年度第3回脂質駆動学術産業創生研究部会講演会, Dec. 2021.
22.	YOSHIDA Kai, Naomi Murakawa, Takaiku Sakamoto and Eiji Sakuradani : 水酸化脂肪酸生産性糸状菌Fusarium solani D2株の形質転換条件の検討, 2021年度第3回脂質駆動学術産業創生研究部会講演会, Dec. 2021.
23.	MASUDA Naoki, Takaiku Sakamoto, 西海信, Takahito Watanabe, Eiji Sakuradani, Taro Mito and Rie Mukai : 食用昆虫の脂質組成分析と実験動物への影響, 2021年度第3回脂質駆動学術産業創生研究部会講演会, Dec. 2021.
24.	Kasumi Nakagawa, 竹内道樹, 菊池真由, Takaiku Sakamoto, 小川順, 加納健司 and Eiji Sakuradani : 藍染液中で起こるインジゴの酸化還元に関わる電子伝達メカニズムの解明, 第22回生体触媒化学シンポジウム, Dec. 2020.
25.	Kasumi Nakagawa, 竹内道樹, 菊池真由, Takaiku Sakamoto, 小川順, 加納健司 and Eiji Sakuradani : 藍染液中のインジゴ還元メカニズムの解明, 日本生物工学会西日本支部大会2020(第5回講演会), Nov. 2020.
26.	KOGA Takeru, Takaiku Sakamoto, Eiji Sakuradani and Akihiro Tai : ヒマワリ種子由来のPC12細胞における神経突起形成促進作用物質, 日本農芸化学会中四国支部第57回講演会(例会), Sep. 2020.
27.	Hiroki Azuma, Kazuhiro Nakamura, Takaiku Sakamoto and Eiji Sakuradani : 油糧糸状菌のSeipin様タンパク質遺伝子の機能解析, 日本農芸化学会中四国支部第57回講演会(例会), Sep. 2020.
28.	KITAGUCHI Naoki, Mao Kusunoki, Takaiku Sakamoto and Eiji Sakuradani : アグロバクテリウム法を用いたシイタケ形質転換法の開発, 日本農芸化学会中四国支部第57回講演会(例会), Sep. 2020.
29.	島田良美, 下平武彦, 相馬悠希, 和泉自泰, 安藤晃規, 岸野重信, Takaiku Sakamoto, 馬場健史, 小川順 and Eiji Sakuradani : 油糧糸状菌Mortierella alpinaの分子育種による遊離脂肪酸生産株のリピドーム解析, 日本農芸化学会2020年度大会, Mar. 2020.
30.	Kasumi Nakagawa, Manami Tada, Takaiku Sakamoto, 竹内道樹, 加納健司, 小川順 and Eiji Sakuradani : 藍染液の電気化学的解析によるインジゴの測定, 日本農芸化学会2020年度大会, Mar. 2020.
31.	Naomi Murakawa, Takaiku Sakamoto, Konomi Ueno, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 糸状菌Fusarium solani D2株由来オレイン酸水和酵素の機能解析, 日本農芸化学会中四国支部第56回講演会(例会), Jan. 2020.
32.	Yusuke Ikeda, Naruho Masuda, 金崎仁志, Takaiku Sakamoto and Eiji Sakuradani : アスタキサンチン生産性ラビリンチュラの探索および育種, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
33.	Yuji Nakamura, Kazuma Eguchi, Takaiku Sakamoto and Eiji Sakuradani : 油糧微生物ラビリンチュラの育種による DHA高生産株の創製, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
34.	Yuka Sakaguchi, Kasumi Nakagawa, Hitomi Takano, Yui Hattori, Takaiku Sakamoto and Eiji Sakuradani : 海洋生物共生菌によるアルキルグリセロール生産, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
35.	Mari Yoshizaki, Tomomi Inoue, Takaiku Sakamoto and Eiji Sakuradani : 灰色かび病菌Botrytis cinerea に抗菌特性を示す微生物の探索, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
36.	Kazuki Nishikawa, Kohki Hanibuchi, Takaiku Sakamoto and Eiji Sakuradani : コール酸資化性菌におけるコール酸代謝経路の解析, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
37.	Konomi Ueno, Naomi Murakawa, Takaiku Sakamoto, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 油糧糸状菌Mortierella alpinaの育種による10-ヒドロキシステアリン酸生産, 日本農芸化学会 2019年度西日本・中四国支部合同大会, Nov. 2019.
38.	Kazuhiro Nakamura, Hiroki Azuma, Takaiku Sakamoto, 島田良美, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 油糧糸状菌Mortierella alpinaにおけるPerilipin様タンパク質の機能解析, 第19回糸状菌分子生物学コンファレンス, Nov. 2019.
39.	Takaiku Sakamoto, Naomi Murakawa, Konomi Ueno, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 糸状菌Fusarium solani D2株から単離した脂肪酸水和酵素の機能解析, 第19回糸状菌分子生物学コンファレンス, Nov. 2019.
40.	Aika Noguchi, Naomi Murakawa, Konomi Ueno, Takaiku Sakamoto and Eiji Sakuradani : 水酸化脂肪酸生産性糸状菌Fusarium solani D2株の形質転換法の開発, 第19回糸状菌分子生物学コンファレンス, Nov. 2019.
41.	Ayae Matsuka, Takaiku Sakamoto, 岡久修己 and Eiji Sakuradani : 自然界より単離した酵母の醸造特性評価, 日本農芸化学会2019年度大会, Mar. 2019.
42.	Naomi Murakawa, Konomi Ueno, Takaiku Sakamoto, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 糸状菌由来オレイン酸水和酵素遺伝子の機能解析, 日本農芸化学会2019年度大会, Mar. 2019.
43.	Kasumi Nakagawa, Manami Tada, Nanoha Nishibayashi, Takaiku Sakamoto, 清藤鈴奈, 竹内道樹, 加納健司, 小川順 and Eiji Sakuradani : 藍染液中の電子伝達に関わる微生物・反応促進物質の探索, 日本農芸化学会2019年度大会, Mar. 2019.
44.	Yuji Nakamura, Takaiku Sakamoto and Eiji Sakuradani : 油糧微生物ラビリンチュラにおけるDHA生産条件の検討, 日本農芸化学会中四国支部第52回講演会, Sep. 2018.
45.	Takumi Otsuka, Takaiku Sakamoto and Eiji Sakuradani : 植物寄生菌の脂質解析, 日本農芸化学会中四国支部第52回講演会, Sep. 2018.
46.	Manami Tada, Kasumi Nakagawa, Takaiku Sakamoto, 清藤鈴奈, 竹内道樹, 小川順 and Eiji Sakuradani : 藍染液より単離した微生物の解析, 日本農芸化学会中四国支部第52回講演会, Sep. 2018.
47.	清藤鈴奈, 竹内道樹, 久郷将見, Manami Tada, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : インジゴ還元菌におけるメディエーターの影響とその機能解析, 日本農芸化学会2018年度大会, Mar. 2018.
48.	Naomi Murakawa, Mizuho Kanoh, Takaiku Sakamoto, 安藤晃規, 岸野重信, 相馬悠希, 馬場健史, 小川順 and Eiji Sakuradani : 廃グリセロール資化性糸状菌が生産する脂肪酸代謝産物, 日本農芸化学会2018年度大会, Mar. 2018.
49.	Ayae Matsuka, Takaiku Sakamoto and Eiji Sakuradani : エタノール生産性天然酵母の単離および諸性質解明, 第9回日本醸造学会若手シンポジウム, Oct. 2017.
50.	Takaiku Sakamoto, Risa Ohta, Minami Kusunoki, Hitomi Takano and Eiji Sakuradani : スクアレン生産性酵母の探索と培養条件の検討, 日本農芸化学会関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会), Sep. 2017.
51.	Mizuho Kanoh, Yuichi Kamegawa, Chinami Kurita, Takaiku Sakamoto and Eiji Sakuradani : 廃グリセロール資化性菌による微生物油脂生産, 日本農芸化学会関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会), Sep. 2017.
52.	Kento Ueda, Takumi Otsuka, Takaiku Sakamoto and Eiji Sakuradani : 冬虫夏草の脂質解析, 日本農芸化学会関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会), Sep. 2017.
53.	Ayae Matsuka, Takaiku Sakamoto and Eiji Sakuradani : 自然界より単離したエタノール生産性酵母の諸性質解明, 日本農芸化学会中四国支部第48回講演会, Jun. 2017.
54.	清藤鈴奈, 竹内道樹, 久郷将見, Kazuya Yomota, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : インジゴ還元能の定量的評価法を用いたインジゴ還元菌の探索及び機能解析, 日本農芸化学会中四国支部第48回講演会, Jun. 2017.
55.	久郷将見, 竹内道樹, Kazuya Yomota, Takaiku Sakamoto, Eiji Sakuradani and 小川順 : インジゴ還元菌の探索及び定量的評価法を用いる機能解析, 日本農芸化学会2017年度大会, Mar. 2017.
56.	Sanae Ide, Takaiku Sakamoto, 安藤晃規, 岸野重信, 和泉自泰, 馬場健史, 島純, 小川順 and Eiji Sakuradani : 油糧糸状菌 Mortierella alpina から誘導したリシノール酸生産変異株について, 日本農芸化学会2017年度大会, Mar. 2017.
57.	Hitomi Takano, Takaiku Sakamoto and Eiji Sakuradani : 海洋性微生物のジピコリン酸生産性評価, 日本農芸化学会2017年度大会, Mar. 2017.
58.	井本篤志, 栗山和也, Takaiku Sakamoto, 左近静香, 泉津弘佑, 鈴木一実 and 入江俊一 : Phanerochaete chrysosporium における aldo-keto還元酵素AKR1, 日本農芸化学会2017年度大会, Mar. 2017.
59.	Eiji Sakuradani, Takaiku Sakamoto, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 島純 and 小川順 : リシノール酸生産を目指した油糧微生物 Mortierella alpina の分子育種, 第7回学際的脂質創生研究部会講演会, Jan. 2017.
60.	和泉自泰, 安藤晃規, Takaiku Sakamoto, 岸野重信, 相馬悠希, 福崎英一郎, Eiji Sakuradani, 島純, 小川順 and 馬場健史 : メタボローム解析による油糧微生物の脂質生産性向上のための代謝改変戦略の立案, 第7回学際的脂質創生研究部会講演会, Jan. 2017.
61.	Naohiro Oka, Takaiku Sakamoto, Eiji Sakuradani, 團昭紀 and Tatsuo Hamano : 有用海藻Agardhiella subulataの脂肪酸分析について, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
62.	Takaiku Sakamoto, Kenshi Morishita, Yasuko Tominaga, Momoka Hamano, 安藤晃規, 岸野重信, 和泉自泰, 馬場健史, 島純, 小川順 and Eiji Sakuradani : 油糧糸状菌Mortierella alpinaの分子育種によるリシノール酸生産, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
63.	Masato Kajiura, Mizuho Kanoh, Yuichi Kamegawa, Chinami Kurita, Takaiku Sakamoto and Eiji Sakuradani : 廃グリセロール資化性菌の探索と微生物油脂生産への応用, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
64.	Kazuya Yomota, Takaiku Sakamoto, Ryuji Oshita, 久郷将見, 竹内道樹, 小川順 and Eiji Sakuradani : インジゴ還元に関わる微生物の探索及び機能性評価, 第3回日本生物工学会西日本支部講演会, Dec. 2016.
65.	富永康子, Momoka Hamano, Takaiku Sakamoto, Sanae Ide, 菊川寛史, 安藤晃規, 岸野重信, 和泉自泰, 馬場健史, 島純, 小川順 and Eiji Sakuradani : リシノール酸高生産株の作製とその脂質解析, 第16回糸状菌分子生物学コンファレンス, Nov. 2016.
66.	Takaiku Sakamoto, 富永康子, Sanae Ide, 奥田知生, 安藤晃規, 岸野重信, 和泉自泰, 馬場健史, 島純, Eiji Sakuradani and 小川順 : 油糧糸状菌におけるリシノール酸生産株の分子育種, 第16回糸状菌分子生物学コンファレンス, Nov. 2016.
67.	左近静香, Takaiku Sakamoto, 泉津弘佑, 鈴木一実 and 入江俊一 : Phanerochaete chrysosporiumにおけるCaM相互作用性アルドケト還元酵素, 日本菌学会第60回大会, Sep. 2016.
68.	Yuichi Kamegawa, Mizuho Kanoh, Chinami Kurita, Takaiku Sakamoto and Eiji Sakuradani : 廃グリセロールを利用した微生物油脂生産, 日本農芸化学会2016年度中四国支部大会, Sep. 2016.
69.	Yuichi Kamegawa, Chinami Kurita, Takaiku Sakamoto and Eiji Sakuradani : グリセロールを利用した微生物油脂生産, 第6回学際的脂質創生研究部会講演会, Jan. 2016.
70.	和泉自泰, 安藤晃規, Takaiku Sakamoto, 光永均, 中尾素直, 岸野重信, 福崎英一郎, Eiji Sakuradani, 島純, 小川順 and 馬場健史 : 油糧糸状菌Mortierella alpinaのメタボローム解析, 第67回日本生物工学会大会, Oct. 2015.
71.	和泉自泰, 安藤晃規, Takaiku Sakamoto, 光永均, 中尾素直, 岸野重信, 福崎英一郎, Eiji Sakuradani, 島純, 小川順 and 馬場健史 : 油糧微生物 Mortierella alpinaのメタボローム解析, 第9回メタボロームシンポジウム, Oct. 2015.
72.	Yusuke Yuasa, Takaiku Sakamoto, 清水昌, 小川順 and Eiji Sakuradani : ステロール生産性 Mortierella alpina の形質転換系の確立, 日本農芸化学会中四国支部第42回講演会, Jun. 2015.
73.	Takaiku Sakamoto, 菊川寛史, 安藤晃規, Eiji Sakuradani, 落合美佐, 島純, 清水昌 and 小川順 : 油糧微生物Mortierella alpina 1S-4株におけるΔ6不飽和化酵素遺伝子をターゲットとした分子育種株の構築, 日本農芸化学会2015年度大会, Mar. 2015.
74.	Takaiku Sakamoto, Eiji Sakuradani, 安藤晃規, 島純 and 小川順 : オレイン酸及びリノール酸高生産を目的としたMortierella alpinaの分子育種, 第14回糸状菌分子生物学コンファレンス, Nov. 2014.
75.	Takaiku Sakamoto, Eiji Sakuradani, 安藤晃規, 島純 and 小川順 : Mortierella alpinaにおけるオレイン酸及びリノール酸高生産株の分子育種, 第66回日本生物工学会大会, Sep. 2014.
76.	Takaiku Sakamoto, Eiji Sakuradani, 安藤晃規, 島純 and 小川順 : オレイン酸及びリノール酸高蓄積性Mortierella alpinaの育種, 生物工学若手研究者の集い, Jul. 2014.
77.	末冨高志, Takaiku Sakamoto, 徳永祥孝, 本田与一, 亀下勇, 鈴木一実, 泉津弘佑 and 入江俊一 : カルモデュリン遺伝子組換えヒラタケにおけるマンガンペルオキシダーゼアイソザイム遺伝子の発現様式, 第64回日本木材学会大会, Mar. 2014.
78.	Takaiku Sakamoto, Eiji Sakuradani, 安藤晃規, 島純 and 小川順 : 油糧微生物Mortierella alpinaにおけるオレイン酸及びリノール酸生産性の向上, 日本農芸化学会2014年度大会, Mar. 2014.
79.	Takaiku Sakamoto, Eiji Sakuradani, 安藤晃規, 島純 and 小川順 : Mortierella alpinaの分子育種によるオレイン酸生産, 第4回学際的脂質創生研究部会講演会, Jan. 2014.
80.	末冨高志, Takaiku Sakamoto, 徳永祥孝, 本田与一, 泉津弘佑, 鈴木一実 and 入江俊一 : ヒラタケのリグニン分解酵素発現調節機構におけるカルモデュリンの役割, 第13回糸状菌分子生物学コンファレンス, Nov. 2013.
81.	菊川寛史, 浅岡卓也, Eiji Sakuradani, 奥田知生, 安藤晃規, Takaiku Sakamoto, 島純 and 小川順 : 油糧微生物Mortierella alpina 1S-4における外来不飽和化酵素遺伝子発現による高度不飽和脂肪酸生産, 第65回日本生物工学会大会, Sep. 2013.
82.	Takaiku Sakamoto, 矢尾祐樹, 本田与一, 鈴木一実 and 入江俊一 : Gタンパク質αサブユニット遺伝子点突然変異がヒラタケのラッカーゼ生産に与える効果, 日本農芸化学会2013年度大会, Mar. 2013.
83.	徳永祥孝, Takaiku Sakamoto, 本田与一, 亀田勇, 鈴木一実 and 入江俊一 : ヒラタケのリグイン分解酵素活性発現におけるカルモデュリン遺伝子の機能, 第63回日本木材学会大会, Mar. 2013.
84.	矢尾祐樹, Takaiku Sakamoto, 岡村沙樹, 本田与一, 鈴木一実 and 入江俊一 : ヒラタケのリグニン分解酵素活性発現におけるサイクリックAMPシグナル伝達関連遺伝子の機能, 第63回日本木材学会大会, Mar. 2013.
85.	熊野翔太, 田村美理, 小篠貴臣, 泉津弘佑, Takaiku Sakamoto, 北浦博法, 鈴木一実 and 入江俊一 : Phanerochaete chrysosporium自然突然変異株由来のカルボキシン耐性遺伝子, 日本農芸化学会2012年度大会, Mar. 2012.
86.	Takaiku Sakamoto, 矢尾祐樹, 上田美波, 本田与一, 亀下勇, 鈴木一実 and 入江俊一 : リグニン分解酵素発現におけるカルモデュリンの働き, 日本農芸化学会2012年度大会, Mar. 2012.
87.	Takaiku Sakamoto, 本田与一, 渡辺隆司, 大山尚毅, 鈴木一実 and 入江俊一 : リグニン分解酵素の発現を調節するカルモデュリン相互作用タンパクの検索, 第61回日本木材学会大会, Mar. 2011.
88.	矢尾祐樹, Takaiku Sakamoto, 橋ヶ谷渉, 肥田嘉文, 鈴木一実, 本田与一, 渡辺隆司 and 入江俊一 : リグニン分解酵素発現におけるcAMPシグナルの作用, 日本農芸化学会2011年度大会, Mar. 2011.
89.	Takaiku Sakamoto, 北浦博法, 南正彦, 上田暁生, 鈴木一実 and 入江俊一 : カルモデュリン阻害条件におけるPhanerochaete chrysosporiumリグニン分解酵素アイソザイム群の発現様式, 第9回糸状菌分子生物学コンファレンス, Nov. 2009.
90.	Takaiku Sakamoto, 北浦博法, 南正彦, 上田暁生, 鈴木一実 and 入江俊一 : Phanerochaete chrysosporiumのリグニン分解酵素発現におけるカルモデュリン阻害剤の影響, 第54回リグニン討論会, Aug. 2009.
91.	Takaiku Sakamoto, 南正彦, 上田暁生, 鈴木一実 and 入江俊一 : Phanerochaete chrysosporiumリグニン分解酵素発現におけるカルモデュリン阻害剤の影響, 日本菌学会第53回大会, Aug. 2009.
92.	南正彦, 鈴木一実, 清水顕史, 大山尚毅, Takaiku Sakamoto and 入江俊一 : LongSAGE法によるPhanerochaete chrysosporiumリグニン分解酵素発現に関与する遺伝子の検索, 第8回糸状菌分子生物学コンファレンス, Nov. 2008.
93.	南正彦, 清水顕史, 鈴木一実, 森真理, 本郷朋博, 大山尚毅, 草加明歩, Takaiku Sakamoto and 入江俊一 : Phanerochaete chrysosporiumリグニン分解酵素発現におけるアトロピン非抑制遺伝子検索, 第57回日本木材学会大会, Aug. 2007.

Et cetera, Workshop:

1.	Takaiku Sakamoto, Naomi Murakawa, Konomi Ueno, 安藤晃規, 岸野重信, 相馬悠希, 和泉自泰, 馬場健史, 小川順 and Eiji Sakuradani : 糸状菌に由来する脂肪酸水和酵素の機能解析, 酵素・補酵素研究会2019, Jul. 2019.
2.	Eiji Sakuradani, Naomi Murakawa and Takaiku Sakamoto : 糸状菌Fusarium sp.由来オレイン酸水和酵素遺伝子の機能解析, 化学コミュニケーションのフロンティア第5回公開シンポジウム, Jun. 2019.
3.	Takaiku Sakamoto : キノコは錬金術師? ∼森の分解と物質生産∼, 日本農芸化学会中四国支部第 28 回若手研究者シンポジウム, Jun. 2018.
4.	Takaiku Sakamoto : もっと知りたいキノコという名の微生物 ∼キノコがつくる薬と毒∼, 公益財団法人国際科学技術財団「やさしい科学技術セミナー」, Oct. 2016.

Patent:

1.	入江俊一, 矢尾祐樹, Takaiku Sakamoto and 鈴木一実 : 真正担子菌のラッカーゼ産生能を増加させる方法, 2012-265662 (Dec. 2012), 2014-108103 (Jun. 2014), .

Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)

Fusarium属糸状菌によるポリマー原料発酵生産機構の解明 (Project/Area Number: 24K08683 )
Elucidation of anaerobic lipid metabolism mechanism of filamentous fungi for polymer production (Project/Area Number: 20K15436 )
Development of a multi-step salt catalyst pressurized microwave treatment system for complete energy conversion of holocellulose (Project/Area Number: 19K22155 )
Development of rare lipid production methods using marine microorganisms symbiotic with the intestinal tracts of marine organisms (Project/Area Number: 17K07723 )
Search by Researcher Number (90740332)

Associate Professor : Sakamoto, Takaiku

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Grants-in-Aid for Scientific Research (KAKEN Grants Database @ NII.ac.jp)