徳島大学 / 教育研究者総覧 --- 粟飯原睦美

個人のホームページ

https://researchmap.jp/1_2 (researchmap)

https://orcid.org/0000-0003-2286-6051 (ORCID)

専門分野

○	ライフサイエンス (Life sciences) [栄養学、健康科学 (Nutrition and health science)]
○	人文・社会 (Humanities & social sciences) [家政学、生活科学 (Home economics, lifestyle science)]

研究テーマ

著書・論文

著書:

1.	宮脇克行, 粟飯原睦美, 髙橋章, 二川健 : LEDを用いた近未来宇宙植物工場の開発, 株式会社技術情報協会, 2020年4月.
2.	池原敏孝, 勢井宏義, 田中弘之, 上番増喬, 北岡和義, 髙橋章, 中橋睦美, 中屋豊, 藤原広明 : 人体生理学の基礎改訂第2版, 医学出版社, 2016年8月. (キーワード) 生理学 (physiology)
3.	粟飯原睦美, 髙橋章 : 近紫外線発光ダイオードを用いた殺菌システム, 化学工業社, 2010年9月.

学術論文(審査論文):

1.	Hanif ALi, Mone Yamanishi, Keigo Sunagawa, Mizuki Kumon, Rumana Yesmin Hasi, Mutsumi Aihara, Ryushi Kawakami and Tamotsu Tanaka : Protective effect of oleic acid against very long-chain fatty acid-induced apoptosis in peroxisome-deficient CHO cells, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1869, No.3, 159452, 2024. (要約) Very long-chain fatty acids (VLCFAs) are degraded exclusively in peroxisomes, as evidenced by the accumulation of VLCFAs in patients with certain peroxisomal disorders. Although accumulation of VLCFAs is considered to be associated with health issues, including neuronal degeneration, the mechanisms underlying VLCFAs-induced tissue degeneration remain unclear. Here, we report the toxic effect of VLCFA and protective effect of C18: 1 FA in peroxisome-deficient CHO cells. We examined the cytotoxicity of saturated and monounsaturated VLCFAs with chain-length at C20-C26, and found that longer and saturated VLCFA showed potent cytotoxicity at lower accumulation levels. Furthermore, the extent of VLCFA-induced toxicity was found to be associated with a decrease in cellular C18:1 FA levels. Notably, supplementation with C18:1 FA effectively rescued the cells from VLCFA-induced apoptosis without reducing the cellular VLCFAs levels, implying that peroxisome-deficient cells can survive in the presence of accumulated VLCFA, as long as the cells keep sufficient levels of cellular C18:1 FA. These results suggest a therapeutic potential of C18:1 FA in peroxisome disease and may provide new insights into the pharmacological effect of Lorenzo's oil, a 4:1 mixture of C18:1 and C22:1 FA. (徳島大学機関リポジトリ) ● Metadata: 118916 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbalip.2024.159452 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38244676 ● Search Scopus @ Elsevier (PMID): 38244676 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2024.159452 (徳島大学機関リポジトリ: 118916, DOI: 10.1016/j.bbalip.2024.159452, PubMed: 38244676)
2.	Nanaka Torigoe, Megumi Nagahara, Thi Suong Nguyen, Qingyi Lin, Koki Takebayashi, Bin Liu, Mutsumi Aihara, Masayasu Taniguchi and Takeshige Otoi : Development of porcine embryos cultured in media irradiated with ultraviolet-C., Reproduction in Domestic Animals = Zuchthygiene, Vol.59, No.1, e14520, 2024. (要約) Sterilization of the culture medium using ultraviolet (UV)-C reduces the potential adverse effects of microorganisms and allows for long-term use. In the present study, we investigated the effects of a medium directly irradiated with UV-C prior to in vitro culture on the development and quality of porcine in vitro-fertilized embryos and the free amino acid composition of the culture media. The culture media (porcine zygote medium [PZM-5] and porcine blastocyst medium [PBM]) were irradiated with UV-C at 228 and 260 nm for 1 and 3 days, respectively. Next, the culture media were irradiated with UV-C at 228 nm for 3, 7, or 14 days. After in vitro fertilization, the embryos were cultured in the UV-C-irradiated media for 7 days. Free amino acid levels in culture media irradiated with 228 and 260 nm UV-C for 3 days were analysed. The blastocyst formation rate of embryos cultured in media irradiated with 260 nm UV-C for 3 days was significantly lower than that of embryos cultured in non-irradiated control media. However, 228 nm UV-C irradiation for up to 14 days did not affect blastocyst formation rates and quality in the resulting blastocysts. Moreover, 260 nm UV-C irradiation significantly increased the taurine concentration in both culture media and decreased methionine concentration in the PBM. In conclusion, UV-C irradiation at 228 nm before in vitro culture had no detrimental effects on embryonic development. However, 260 nm UV-C irradiation decreased embryo development and altered the composition of free amino acids in the medium. (出版サイトへのリンク) ● Publication site (DOI): 10.1111/rda.14520 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38268205 ● Search Scopus @ Elsevier (PMID): 38268205 ● Search Scopus @ Elsevier (DOI): 10.1111/rda.14520 (DOI: 10.1111/rda.14520, PubMed: 38268205)
3.	Yoshimichi Takai, Rumana Yesmin Hasi, Naoko Matsumoto, Chiho Fujita, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Toshiki Ishikawa, Hiroyuki Imai, Mayuko Wakida, Kazuya Ando and Tamotsu Tanaka : Degradation of glycosylinositol phosphoceramide during plant tissue homogenization, The Journal of Biochemistry, Vol.175, No.1, 115-124, 2024. (要約) A convenient method for the determination of plant sphingolipids (glycosylinositol phosphoceramide, GIPC; glucosylceramide, GluCer; phytoceramide 1-phosphate, PC1P and phytoceramide, PCer) was developed. This method includes the extraction of lipids using 1-butanol, alkali hydrolysis with methylamine and separation by TLC. The amounts of sphingolipids in the sample were determined based on the relative intensities of standard sphingolipids visualized by primulin/UV on TLC. Using this method, we found that almost all GIPCs were degraded in response to tissue homogenization in cruciferous plants (cabbage, broccoli and Arabidopsis thaliana). The decrease in GIPCs was compensated for by increases in PC1P and PCer, indicating that GIPC was degraded by hydrolysis at the D and C positions of GIPC, respectively. In carrot roots and leaves, most of GIPC degradation was compensated for by an increase in PCer. In rice roots, the decrease in GIPCs was not fully explained by the increases in PC1P and PCer, indicating that enzymes other than phospholipase C and D activities operated. As the visualization of lipids on TLC is useful for detecting the appearance or disappearance of lipids, this method will be available for the characterization of metabolism of sphingolipids in plants. (キーワード) Glycosphingolipids / Sphingolipids / Plants / Brassica / Arabidopsis (徳島大学機関リポジトリ) ● Metadata: 118914 (出版サイトへのリンク) ● Publication site (DOI): 10.1093/jb/mvad080 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37827526 ● CiNii @ 国立情報学研究所 (CRID): 1520862036472955648 ● Summary page in Scopus @ Elsevier: 2-s2.0-85181760535 (徳島大学機関リポジトリ: 118914, DOI: 10.1093/jb/mvad080, PubMed: 37827526, CiNii: 1520862036472955648, Elsevier: Scopus)
4.	Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Mutsumi Aihara, Masahiro Nii and Takeshige Otoi : Effects of curcumin supplementation on quality of porcine spermatozoa irradiated with ultraviolet-C at 228 nm during liquid preservation., Reproduction in Domestic Animals = Zuchthygiene, 2023. (要約) It is important to prevent microbial contamination during liquid preservation of semen in pigs. We examined the effects of curcumin supplementation on the quality of porcine spermatozoa irradiated with ultraviolet-C (UV-C) at 228 nm. UV-C is used to disinfect gases and solid surfaces. In the first experiment, porcine semen was preserved with 0, 10, 25, 50 or 100 μM curcumin under UV-C irradiation at 228 nm for 7 days at 15°C. The irradiation did not affect the motility and viability of preserved spermatozoa but decreased the percentage of plasma membrane integrity of spermatozoa. Curcumin supplementation at 25 μM significantly improved the plasma membrane and acrosome integrity of irradiated spermatozoa compared with spermatozoa preserved without curcumin (p < .05). In the second experiment, semen was preserved with or without 25 μM curcumin with UV-C irradiation at 228 or 260 nm for 3 days at 15°C. Curcumin supplementation increased the percentages of total motility, sperm viability and plasma membrane integrity of preserved spermatozoa at both irradiation wavelengths (p < .05). All quality parameters of 260 nm irradiated spermatozoa decreased compared to those of the other groups, irrespective of curcumin supplementation. The collective findings indicate that porcine spermatozoa can retain their viability even after continuous long-duration irradiation with 228 nm UV-C. Curcumin supplementation improves the quality of UV-C irradiated spermatozoa during semen preservation. (出版サイトへのリンク) ● Publication site (DOI): 10.1111/rda.14362 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37081592 ● Search Scopus @ Elsevier (PMID): 37081592 ● Search Scopus @ Elsevier (DOI): 10.1111/rda.14362 (DOI: 10.1111/rda.14362, PubMed: 37081592)
5.	Qingyi Lin, Mutsumi Aihara, Akihiro Shirai, Ami Tanaka, Koki Takebayashi, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Takeo Minamikawa and Takeshige Otoi : Porcine embryo development and inactivation of microorganisms after ultraviolet-C irradiation at 228 nm, Theriogenology, Vol.197, 252-258, 2023. (要約) It is important to prevent contamination inside the incubator as a method of preventing microbial infections during the embryo culture. In the present study, we examined the effects of ultraviolet-C (UV-C) irradiation, used for microorganism inactivation, on embryo development and the growth of bacteria, including Escherichia coli and Staphylococcus aureus, and the fungus Cladosporium cladosporioides. In the embryo irradiation experiment, we examined the effects of the plastic lid of the culture dish, irradiation distances (10, 20, and 25 cm), and different irradiation wavelengths (228 and 260 nm) during embryo culture for 7 days on the development and quality of porcine in vitro-fertilized embryos. None of the embryos cultured in dishes without plastic lids developed into blastocysts after irradiation with 228 nm UV-C. When porcine embryos were cultured in a culture dish with lids, the 228 nm UV-C irradiation decreased blastocyst formation rates of the embryos but not their quality, irrespective of the UV-C irradiation distance. Moreover, irradiation with 260 nm UV-C, even with plastic lids, had more detrimental effects on embryo development than irradiation with 228 nm UV-C. Investigation of the inactivating effects of UV-C irradiation at 228 nm and 260 nm on the growth of the bacteria and fungus showed that 260 nm UV-C reduced the viability to a greater extent than 228 nm UV-C. Moreover, the disinfection efficacy for the bacteria increased when the irradiation duration increased and the distance decreased. In conclusion, porcine embryos can develop into blastocysts without loss of quality even after continuous long-duration irradiation (7 days) with 228 nm UV-C, which can inactivate the growth of bacteria and the tested fungus; however, the development rate of the embryo is reduced. (キーワード) Animals / Swine / Embryonic Development / Blastocyst / Embryo, Mammalian / Fertilization in Vitro / Escherichia coli / Bacteria / Ultraviolet Rays (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.theriogenology.2022.12.015 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36525864 ● Search Scopus @ Elsevier (PMID): 36525864 ● Search Scopus @ Elsevier (DOI): 10.1016/j.theriogenology.2022.12.015 (DOI: 10.1016/j.theriogenology.2022.12.015, PubMed: 36525864)
6.	Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka : Peroxisomes attenuate cytotoxicity of very long-chain fatty acids, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1868, No.2, 159259, 2023. (要約) One of the major functions of peroxisomes in mammals is oxidation of very long-chain fatty acids (VLCFAs). Genetic defects in peroxisomal β-oxidation result in the accumulation of VLCFAs and lead to a variety of health problems, such as demyelination of nervous tissues. However, the mechanisms by which VLCFAs cause tissue degeneration have not been fully elucidated. Recently, we found that the addition of small amounts of isopropanol can enhance the solubility of saturated VLCFAs in an aqueous medium. In this study, we characterized the biological effect of extracellular VLCFAs in peroxisome-deficient Chinese hamster ovary (CHO) cells, neural crest-derived pheochromocytoma cells (PC12), and immortalized adult Fischer rat Schwann cells (IFRS1) using this solubilizing technique. C20:0 FA was the most toxic of the C16-C26 FAs tested in all cells. The basis of the toxicity of C20:0 FA was apoptosis and was observed at 5 μM and 30 μM in peroxisome-deficient and wild-type CHO cells, respectively. The sensitivity of wild-type CHO cells to cytotoxic C20:0 FA was enhanced in the presence of a peroxisomal β-oxidation inhibitor. Further, a positive correlation was evident between cell toxicity and the extent of intracellular accumulation of toxic FA. These results suggest that peroxisomes are pivotal in the detoxification of apoptotic VLCFAs by preventing their accumulation. (キーワード) Cricetinae / Animals / Peroxisomes / Fatty Acids / CHO Cells / Cricetulus / Oxidation-Reduction (徳島大学機関リポジトリ) ● Metadata: 117825 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbalip.2022.159259 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36460260 ● Search Scopus @ Elsevier (PMID): 36460260 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2022.159259 (徳島大学機関リポジトリ: 117825, DOI: 10.1016/j.bbalip.2022.159259, PubMed: 36460260)
7.	Retsuo Kawakami, Mutsumi Aihara, Takuto Izumi, Akihiro Shirai and Mukai Takashi : Bactericidal Effects of Low-Temperature Atmospheric-Pressure Air Plasma Jets with No Damage to Plant Nutrient Solutions, Biochemical Engineering Journal, Vol.187, 108661:1-108661:9, 2022. (徳島大学機関リポジトリ) ● Metadata: 117580 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bej.2022.108661 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-85139727087 (徳島大学機関リポジトリ: 117580, DOI: 10.1016/j.bej.2022.108661, Elsevier: Scopus)
8.	Rumana Yesmin Hasi, Toshiki Ishikawa, Keigo Sunagawa, Yoshimichi Takai, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Keizo Yuasa, Mutsumi Aihara, Kaori Kanemaru, Hiroyuki Imai and Tamotsu Tanaka : Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide, FEBS Letters, Vol.596, No.23, 3024-3036, 2022. (要約) Glycosylinositol phosphoceramide (GIPC) is a major sphingolipid in the plasma membranes of plants. Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of the D position of GIPC in cabbage and named this activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as a PLD. The recombinant NPC3 protein obtained by heterologous expression system in Escherichia coli produced PC1P from GIPC and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish and Arabidopsis thaliana. From these results, we conclude that NPC3 is one of the enzymes that degrade GIPC. (キーワード) Phospholipase D / Raphanus / Phospholipases / Sphingolipids / Brassica / Arabidopsis (徳島大学機関リポジトリ) ● Metadata: 117824 (出版サイトへのリンク) ● Publication site (DOI): 10.1002/1873-3468.14520 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36266963 ● Search Scopus @ Elsevier (PMID): 36266963 ● Search Scopus @ Elsevier (DOI): 10.1002/1873-3468.14520 (徳島大学機関リポジトリ: 117824, DOI: 10.1002/1873-3468.14520, PubMed: 36266963)
9.	Ali Hanif, Morito Katsuya, Rumana Hasi Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Sango Kazunori and Tamotsu Tanaka : Characterization of uptake and metabolism of very long-chain fatty acids in peroxisome-deficient CHO cells, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1867, No.2, 159088, 2022. (要約) Fatty acids (FAs) longer than C20 are classified as very long-chain fatty acids (VLCFAs). Although biosynthesis and degradation of VLCFAs are important for the development and integrity of the myelin sheath, knowledge on the incorporation of extracellular VLCFAs into the cells is limited due to the experimental difficulty of solubilizing them. In this study, we found that a small amount of isopropanol solubilized VLCFAs in aqueous medium by facilitating the formation of the VLCFA/albumin complex. Using this solubilizing technique, we examined the role of the peroxisome in the uptake and metabolism of VLCFAs in Chinese hamster ovary (CHO) cells. When wild-type CHO cells were incubated with saturated VLCFAs (S-VLCFAs), such as C23:0 FA, C24:0 FA, and C26:0 FA, extensive uptake was observed. Most of the incorporated S-VLCFAs were oxidatively degraded without acylation into cellular lipids. In contrast, in peroxisome-deficient CHO cells uptake of S-VLCFAs was marginal and oxidative metabolism was not observed. Extensive uptake and acylation of monounsaturated (MU)-VLCFAs, such as C24:1 FA and C22:1 FA, were observed in both types of CHO cells. However, oxidative metabolism was evident only in wild-type cells. Similar manners of uptake and metabolism of S-VLCFAs and MU-VLCFAs were observed in IFRS1, a Schwan cell-derived cell line. These results indicate that peroxisome-deficient cells limit intracellular S-VLCFAs at a low level by halting uptake, and as a result, peroxisome-deficient cells almost completely lose the clearance ability of S-VLCFAs accumulated outside of the cells. (徳島大学機関リポジトリ) ● Metadata: 116579 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbalip.2021.159088 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34848380 ● CiNii @ 国立情報学研究所 (CRID): 1360013168847983360 ● Search Scopus @ Elsevier (PMID): 34848380 ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2021.159088 (徳島大学機関リポジトリ: 116579, DOI: 10.1016/j.bbalip.2021.159088, PubMed: 34848380, CiNii: 1360013168847983360)
10.	Tamotsu Tanaka, Kazuya Koyama, Naoko Takahashi, Katsuya Morito, Hanif Ali, Momoyo Azuma, Kozo Kagawa, Hiroshi Kawano, Rumana Yesmin, Mutsumi Aihara and Yasuhiko Nishioka : Lysophosphatidic acid, ceramide 1-phosphate and sphingosine 1-phosphate in peripheral blood of patients with idiophathic pulmonary fibrosis, The Journal of Medical Investigation : JMI, Vol.69, No.3.4, 196-203, 2022. (要約) Idiopathic pulmonary fibrosis (IPF) is the most common idiopathic interstitial pneumonias. Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are signaling lipids that evoke growth factor-like responses to many cells. Recent studies revealed the involvement of LPA and S1P in the pathology of IPF. In this study, we determined LPA, S1P and ceramide 1-phosphate (C1P) in peripheral blood plasma of IPF patients, and examined correlation to the vital capacity of lung (VC), an indicator of development of fibrosis. Blood plasma samples were taken from eleven patients with IPF and seven healthy volunteers. The lipids of the sample were extracted and subjected to liquid chromatography-tandem mass spectrometry for analysis. Results showed that there is a significant negative correlation between VC and plasma LPA levels, indicating that IPF patients with advanced fibrosis had higher concentration of LPA in their plasma. Average of S1P levels were significantly higher in IPF patients than those in healthy subjects. Although it is not statistically significant, a similar correlation trend that observed in LPA levels also found between VC and S1P levels. These results indicated that plasma LPA and S1P may be associated with deterioration of pulmonary function of IPF patients. J. Med. Invest. 69 : 196-203, August, 2022. (キーワード) Ceramides / Fibrosis / Humans / Idiopathic Pulmonary Fibrosis / Lysophospholipids / Sphingosine (徳島大学機関リポジトリ) ● Metadata: 117621 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.69.196 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36244770 ● Search Scopus @ Elsevier (PMID): 36244770 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.69.196 (徳島大学機関リポジトリ: 117621, DOI: 10.2152/jmi.69.196, PubMed: 36244770)
11.	Hanif Ali, Ryouhei Yamashita, Jun-ichi Morishige, Katsuya Morito, Naoya Kakiuchi, Junji Hayashi, Mutsumi Aihara, Ryushi Kawakami, Koichiro Tsuchiya and Tamotsu Tanaka : Massspectrometric analysis of sphingomyelin with N-alfa-hydroxy fatty acyl residue in mouse tissues, Lipids, Vol.56, No.2, 181-188, 2021. (徳島大学機関リポジトリ) ● Metadata: 117826 (出版サイトへのリンク) ● Publication site (DOI): 10.1002/lipd.12285 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.1002/lipd.12285 (徳島大学機関リポジトリ: 117826, DOI: 10.1002/lipd.12285)
12.	Miki Yasui-Maetani, Kazuaki Mawatari, Airi Honjo, Bui Kim Thi Ngan, Takaaki Shimohata, Takashi Uebanso, Mutsumi Aihara, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu, Applied Sciences, Vol.10, No.16, 2020. (徳島大学機関リポジトリ) ● Metadata: 115319 (出版サイトへのリンク) ● Publication site (DOI): 10.3390/app10165549 (文献検索サイトへのリンク) ● Search Scopus @ Elsevier (DOI): 10.3390/app10165549 (徳島大学機関リポジトリ: 115319, DOI: 10.3390/app10165549)
13.	Takaaki Shimohata, Kazuaki Mawatari, Takashi Uebanso, Airi Honjo, Akari Tsunedomi, Sho Hatayama, Yuri Sato, Junko Kido, Risa Nishisaka, Ayumi Yoshimoto, Tomoko Yamashita, Sachie Amano, Miki Maetani-Yasui, Hitomi Iba, Yumi Harada, Mutsumi Nakahashi, Sonoko Yasui-Yamada, Yasuhiro Hamada, Tadahiko Nakagawa, Masahiro Sogabe, Takahiro Emoto, Masatake Akutagawa, Toshiya Okahisa, Yohsuke Kinouchi and Akira Takahashi : Bacterial Contamination of Hemodialysis Devices in Hospital Dialysis Wards., The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 148-152, 2019. (要約) Chronic care patients undergoing hemodialysis for treatment of end-stage renal failure experience higher rates of bloodstream-associated infection due to the patients' compromised immune system and management of the bloodstream through catheters. Staphylococcus species are acommon cause of hemodialysis catheterrelated bloodstream infections. We investigated environmental bacterial contamination of dialysis wards and contamination of hemodialysis devices to determine the source of bacteria for these infections. All bacterial samples were collected by the swab method and the agarose stamp method. And which bacterium were identified by BBL CRYSTAL Kit or 16s rRNA sequences. In our data, bacterial cell number of hemodialysis device was lower than environment of patient surrounds. But Staphylococcus spp. were found predominantly on the hemodialysis device (46.8%), especially on areas frequently touched by healthcare-workers (such as Touch screen). Among Staphylococcus spp., Staphylococcus epidermidis was most frequently observed (42.1% of Staphylococcus spp.), and more surprising, 48.2% of the Staphylococcus spp. indicated high resistance for methicillin. Our finding suggests that hemodialysis device highly contaminated with bloodstream infection associated bacteria. This study can be used as a source to assess the risk of contamination-related infection and to develop the cleaning system for the better prevention for bloodstream infections in patients with hemodialysis. J. Med. Invest. 66 : 148-152, February, 2019. (徳島大学機関リポジトリ) ● Metadata: 113411 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.66.148 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064928 ● Search Scopus @ Elsevier (PMID): 31064928 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.148 (徳島大学機関リポジトリ: 113411, DOI: 10.2152/jmi.66.148, PubMed: 31064928)
14.	Sho Hatayama, Takaaki Shimohata, Sachie Amano, Junko Kido, Q Anh Nguyen, Yuri Sato, Yuna Kanda, Aya Tentaku, Shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells., Frontiers in Cellular and Infection Microbiology, Vol.8, No.30, 2018. (要約) Campylobacter jejuni invasion is closely related to C. jejuni pathogenicity. The intestinal epithelium contains polarized epithelial cells that form tight junctions (TJs) to provide a physical barrier against bacterial invasion. Previous studies indicated that C. jejuni invasion of non-polarized cells involves several cellular features, including lipid rafts. However, the dynamics of C. jejuni invasion of polarized epithelial cells are not fully understood. Here we investigated the interaction between C. jejuni invasion and TJ formation to characterize the mechanism of C. jejuni invasion in polarized epithelial cells. In contrast to non-polarized epithelial cells, C. jejuni invasion was not affected by depletion of lipid rafts in polarized epithelial cells. However, depletion of lipid rafts significantly decreased C. jejuni invasion in TJ disrupted cells or basolateral infection and repair of cellular TJs suppressed lipid raft-mediated C. jejuni invasion in polarized epithelial cells. In addition, pro-inflammatory cytokine, TNF- treatment that induce TJ disruption promote C. jejuni invasion and lipid rafts depletion significantly reduced C. jejuni invasion in TNF- treated cells. These data demonstrated that TJs prevent C. jejuni invasion from the lateral side of epithelial cells, where they play a main part in bacterial invasion and suggest that C. jejuni invasion could be increased in inflammatory condition. Therefore, maintenance of TJs integrity should be considered important in the development of novel therapies for C. jejuni infection. (徳島大学機関リポジトリ) ● Metadata: 111607 (出版サイトへのリンク) ● Publication site (DOI): 10.3389/fcimb.2018.00015 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29441328 ● Search Scopus @ Elsevier (PMID): 29441328 ● Search Scopus @ Elsevier (DOI): 10.3389/fcimb.2018.00015 (徳島大学機関リポジトリ: 111607, DOI: 10.3389/fcimb.2018.00015, PubMed: 29441328)
15.	Ayumi Yoshimoto, Takashi Uebanso, Mutsumi Nakahashi, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi : Effect of prenatal administration of low dose antibiotics on gut microbiota and body fat composition of newborn mice., Journal of Clinical Biochemistry and Nutrition, Vol.62, No.2, 155-160, 2017. (要約) Several environmental factors during the prenatal period transgenerationally affect the health of newborns in later life. Because low-dose antibiotics have been used for promoting the growth of crops and livestock in agriculture, humans may have ingested residual antibiotics for several decades. However, the effect of prenatal administration of low-dose antibiotics on newborns' health in later life is unclear. In the present study, we found that prenatal treatment of murine mothers with low-dose antibiotics increased the abundance of bacteria of the phylum Firmicutes and the genera Clostridium IV and XIVa in feces from pups. In addition, the body fat percentage of mice in the antibiotic-treated group was higher than those in the control group at 12 weeks of age even though all pups were fed a standard diet. The body fat percentage of all mice was correlated with the abundance of fecal bacteria of Clostridium IV and XIVa. These results predict that low-dose antibiotic administration during the prenatal period affects the gut microbiota of newborns and possibly their health in later life. (徳島大学機関リポジトリ) ● Metadata: 114647 (出版サイトへのリンク) ● Publication site (DOI): 10.3164/jcbn.17-53 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29610555 ● Search Scopus @ Elsevier (PMID): 29610555 ● Search Scopus @ Elsevier (DOI): 10.3164/jcbn.17-53 (徳島大学機関リポジトリ: 114647, DOI: 10.3164/jcbn.17-53, PubMed: 29610555)
16.	Junko Kido, Takaaki Shimohata, Sachie Amano, Syo Hatayama, Quoc Anh Nguyen, Yuri Sato, Yuna Kanda, Aya Tentaku, Shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : CFTR reduces microtubule-dependent Campylobacter jejuni invasion., Infection and Immunity, 2017. (要約) Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection. (徳島大学機関リポジトリ) ● Metadata: 114676 (出版サイトへのリンク) ● Publication site (DOI): 10.1128/IAI.00311-17 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28784926 ● Search Scopus @ Elsevier (PMID): 28784926 ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.00311-17 (徳島大学機関リポジトリ: 114676, DOI: 10.1128/IAI.00311-17, PubMed: 28784926)
17.	Takashi Uebanso, Ai Ohnishi, Reiko Kitayama, Ayumi Yoshimoto, Mutsumi Nakahashi, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi : Effects of Low-Dose Non-Caloric Sweetener Consumption on Gut Microbiota in Mice., Nutrients, Vol.9, No.6, 2017. (要約) Abstract: Non-caloric artificial sweeteners (NASs) provide sweet tastes to food without adding calories or glucose. NASs can be used as alternative sweeteners for controlling blood glucose levels and weight gain. Although the consumption of NASs has increased over the past decade in Japan and other countries, whether these sweeteners affect the composition of the gut microbiome is unclear. In the present study, we examined the effects of sucralose or acesulfame-K ingestion (at most the maximum acceptable daily intake (ADI) levels, 15 mg/kg body weight) on the gut microbiome in mice. Consumption of sucralose, but not acesulfame-K, for 8 weeks reduced the relative amount of Clostridiumcluster XIVa in feces. Meanwhile, sucralose and acesulfame-K did not increase food intake, body weight gain or liver weight, or fat in the epididymis or cecum. Only sucralose intake increased the concentration of hepatic cholesterol and cholic acid. Moreover, the relative concentration of butyrate and the ratio of secondary/primary bile acids in luminal metabolites increased with sucralose consumption in a dose-dependent manner. These results suggest that daily intake of maximum ADI levels of sucralose, but not acesulfame-K, affected the relative amount of the Clostridium cluster XIVa in fecal microbiome and cholesterol bile acid metabolism in mice. (徳島大学機関リポジトリ) ● Metadata: 115661 (出版サイトへのリンク) ● Publication site (DOI): 10.3390/nu9060560 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28587159 ● Search Scopus @ Elsevier (PMID): 28587159 ● Search Scopus @ Elsevier (DOI): 10.3390/nu9060560 (徳島大学機関リポジトリ: 115661, DOI: 10.3390/nu9060560, PubMed: 28587159)
18.	Yanfei Hou, Mutsumi Nakahashi, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Yumi Harada, Akari Tsunedomi, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Combined treatment of UVA irradiation and antibiotics induces greater bactericidal effects on Vibrio parahaemolyticus., The Journal of Medical Investigation : JMI, Vol.63, No.1-2, 63-67, 2016. (要約) The presence of antibiotics in the environment and their subsequent impact on the development of multi-antibiotic resistant bacteria has raised concerns globally. Consequently, much research is focused on a method to produce a better disinfectant. We have established a disinfectant system using UVA-LED that inactivates pathogenic bacteria. We assessed the bactericidal efficiency of a combination of UVA-LED and antibiotics against Vibrio parahaemolyticus. Combined use of antibiotic drugs and UVA irradiation was more bactericidal than UVA irradiation or antibacterial drugs alone. The bactericidal synergy was observed at low concentrations of each drug that are normally unable to kill the bacteria. This combination has the potential to become a sterilization technology. J. Med. Invest. 63: 63-67, February, 2016. (徳島大学機関リポジトリ) ● Metadata: 111155 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.63.63 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27040055 ● Search Scopus @ Elsevier (PMID): 27040055 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.63.63 (徳島大学機関リポジトリ: 111155, DOI: 10.2152/jmi.63.63, PubMed: 27040055)
19.	Quang Ngoc Phan, Takashi Uebanso, Takaaki Shimohata, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi : DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus., Journal of Bacteriology, 2015. (要約) Nucleoid binding protein HU regulate cellular behaviors, including nucleoid structuring, general recombination, transposition, growth, replication, motility, metabolism, and virulence. It is concerned that both number of bacteria and number of virulence factors may affect pathogenecity of bacteria. In the present study, we investigated that which factor have dominant role during infection in one of the most rapidly growing bacteria Vibrio parahaemolyticus. We found that V. parahaemolyticus cytotoxicity is regulated, in a growth rate-independent manner, by the HU proteins through regulation of number of virulence factors including T3SS1 gene expression. (徳島大学機関リポジトリ) ● Metadata: 109503 (出版サイトへのリンク) ● Publication site (DOI): 10.1128/JB.00306-15 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26148713 ● Search Scopus @ Elsevier (PMID): 26148713 ● Search Scopus @ Elsevier (DOI): 10.1128/JB.00306-15 (徳島大学機関リポジトリ: 109503, DOI: 10.1128/JB.00306-15, PubMed: 26148713)
20.	Toshitaka Ikehara, Mutsumi Nakahashi, Zehong Su, Masatake Akutagawa, Koichiro Tsuchiya, Mitsuo Kitamura, Akira Takahashi and Yohsuke Kinouchi : Effects of UV-A LED light irradiation on growth of cultured RAW 264.7 cells, Toxicological and Environmental Chemistry, Vol.97, No.2, 243-255, 2015. (キーワード) 365 nm / LED light / 活性酸素 (reactive oxygen species) / Raw 264.7 cells / scavenger (出版サイトへのリンク) ● Publication site (DOI): 10.1080/02772248.2015.1039771 (文献検索サイトへのリンク) ● Summary page in Scopus @ Elsevier: 2-s2.0-84931571408 (DOI: 10.1080/02772248.2015.1039771, Elsevier: Scopus)
21.	Sachie Negoro, Takaaki Shimohata, Syo Hatayama, Yuri Sato, Mari Matsumoto, Hitomi Iba, Mutsumi Aihara, Takashi Uebanso, Yasuhiro Hamada, Yoshikazu Nishikawa, Shinji Yamasaki, Kazuaki Mawatari and Akira Takahashi : Campylobacter jejuni infection suppressed Cl(-) secretion induced by CFTR activation in T-84 cells., Journal of Infection and Chemotherapy, Vol.20, No.11, 682-688, 2014. (要約) Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl(-)) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl(-) channel transporting Cl(-) to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied. Here, we used an (125)I(-) efflux assay and measurement of short-circuit current to measure Cl(-) secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl(-) secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl(-) secretion suppressed in C. jejuni-infected T-84 cells. The suppression of activated Cl(-) secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract. (徳島大学機関リポジトリ) ● Metadata: 110108 (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.jiac.2014.07.007 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25107576 ● Summary page in Scopus @ Elsevier: 2-s2.0-84908232552 (徳島大学機関リポジトリ: 110108, DOI: 10.1016/j.jiac.2014.07.007, PubMed: 25107576, Elsevier: Scopus)
22.	Mutsumi Nakahashi, Kazuaki Mawatari, Akiko Hirata, Miki Maetani, Takaaki Shimohata, Takashi Uebanso, Yasuhiro Hamada, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus., Photochemistry and Photobiology, Vol.90, No.6, 1397-1403, 2014. (要約) Ultraviolet (UV) irradiation is an increasingly used method of water disinfection. UV rays can be classified by wavelength into UVA (320-400 nm), UVB (280-320 nm), and UVC (< 280 nm). We previously developed UVA sterilization equipment with a UVA-light emitting diode (LED). The aim of this study was to establish a new water disinfection procedure using the combined irradiation of the UVA-LED and another UV wavelength. An oxidative DNA product, 8-hydroxy-2'-deoxyguanosine (8-OHdG), increased after irradiation by UVA-LED alone, and the level of cyclobutane pyrimidine dimers (CPDs) was increased by UVC alone in Vibrio parahaemolyticus. Although sequential irradiation of UVA-LED and UVC induced additional bactericidal effects, simultaneous irradiation with UVA-LED and UVC induced bactericidal synergistic effects. 8-OHdG and CPDs production showed no differences between sequential and simultaneous irradiation. Interestingly, the recovery of CPDs was delayed by simultaneous irradiation. The synergistic effect was absent in SOS response-deficient mutants, such as the recA and lexA strains. Because recA- and lexA-mediated SOS responses have crucial roles in a DNA repair pathway, the synergistic bactericidal effect produced by the simultaneous irradiation could depend on suppression of the CPDs repair. The simultaneous irradiation of UVA-LED and UVC is a candidate new procedure for effective water disinfection. This article is protected by copyright. All rights reserved. (出版サイトへのリンク) ● Publication site (DOI): 10.1111/php.12309 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25041035 ● Summary page in Scopus @ Elsevier: 2-s2.0-84915794877 (DOI: 10.1111/php.12309, PubMed: 25041035, Elsevier: Scopus)
23.	Akihiro Shirai, Mutsumi Aihara, Akira Takahashi, Hideaki Maseda and Takeshi Omasa : Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet A light, Journal of Photochemistry and Photobiology B: Biology, Vol.130, 226-233, 2014. (要約) This study examined the utility of synergistic disinfection employing a gemini-quaternary ammonium salt (a gemini-QUAT, namely 3,3'-(2,7-dioxaoctane)bis(1-decylpyridinium bromide)), as an organic biocide in combination with irradiation by an ultraviolet-A (UV-A) light-emitting diode (LED) with a peak wavelength of 365nm. The combined system represents a novel disinfection method utilizing facilitated in situ oxidation depending on overproduction of reactive oxygen species (ROS) triggered by the initial action of the gemini-QUAT on the bacterial membrane. We demonstrate that this combination decreased the viability of pathogenic bacteria in a significant and rapid manner, and depended on doses of the gemini-QUAT and the fluence: the viability of Escherichia coli was reduced by greater than 5.0-logs by the combination procedure, but the decrease in viability was only 2.3-logs for exposure to UV at the same fluence dose in the absence of the gemini-QUAT. Adding catalase as a radical scavenger decreased bacterial inactivation by the combined disinfection procedure. Flow cytometric analysis indicated superoxide and hydrogen peroxide overproduction within cells treated with the combined disinfection procedure. The excessive superoxide, detected only in the combined system, appeared to be generated by the action of the gemini-QUAT at the bacterial membrane, leading to excessive and rapid generation of ROS in the system. Our data strongly suggested that this ROS promoted bacterial membrane peroxidation during initial treatment by the combination method, resulting in increased oxidative modification of DNA. These oxidative reactions may play an important role in the efficacy of this disinfection procedure. (キーワード) Anti-Bacterial Agents / Bacteria / Bacterial Load / DNA / Lipid Peroxidation / Pyridinium Compounds / Quaternary Ammonium Compounds / Reactive Oxygen Species / Ultraviolet Rays (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.jphotobiol.2013.11.027 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24362318 ● Summary page in Scopus @ Elsevier: 2-s2.0-84890840874 (DOI: 10.1016/j.jphotobiol.2013.11.027, PubMed: 24362318, Elsevier: Scopus)
24.	Mutsumi Aihara, Xin Lian, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari, Yumi Harada, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Vegetable surface sterilization system using UVA light-emitting diodes., The Journal of Medical Investigation : JMI, Vol.61, No.3-4, 285-290, 2014. (要約) Surface sterilization of fresh produce has been needed in the food manufacturing/processing industry. Here we report a UVA-LED (Ultra Violet A-Light Emitting Diode) system for surface sterilization that is safe, efficacious, low cost, and apparently harmless to fresh produce. To test the system, Escherichia coli strain DH5 was spot-inoculated onto vegetable tissues, and treated under UVA-LED. Tissues were homogenized and bacteria quantified by colony-forming assay. Possible effects of UVA-LED on vegetable quality were evaluated by HPLC. Tissue weight changes were checked after treatment at 4, 15, and 30. Bacterial inactivation by UVA-LED radiation was observed after a 10 min treatment and increased with increasing time of irradiation. The log survival ratio reached -3.23 after a 90 min treatment. Bacterial cells surviving treatment grew slowly compared to non-irradiated control cells. Cabbage tissue lost weight over time after treatment, and weight loss increased with increasing incubation temperature, but there was no difference between losses by UVA-LED treated and control tissues at any temperature tested. In addition, no differences of Vitamin C content in cabbage tissue were detected by HPLC after UVA-LED treatment. These results suggest that UVA-LED treatment has great potential for vegetable surface sterilization in the food manufacturing/processing industry. (徳島大学機関リポジトリ) ● Metadata: 109573 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.61.285 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25264046 ● Summary page in Scopus @ Elsevier: 2-s2.0-84907509465 (徳島大学機関リポジトリ: 109573, DOI: 10.2152/jmi.61.285, PubMed: 25264046, Elsevier: Scopus)
25.	Hirokazu Takechi, Kazuaki Mawatari, Nagakatsu Harada, Yutaka Nakaya, Megumi Asakura, Mutsumi Aihara, Hiromitsu Takizawa, Masakazu Goto, Takeshi Nishino, Takuya Minato, Yoshihito Furukita, Yota Yamamoto, Yasuhiro Yuasa, Hiromichi Yamai, Takahiro Yoshida, Jun-ichi Seike and Akira Tangoku : Glutamine protects the small intestinal mucosa in anticancer drug-induced rat enteritis model., The Journal of Medical Investigation : JMI, Vol.61, No.1-2, 59-64, 2014. (要約) Supportive therapy during chemotherapy has become essential, but effective preventive therapies to gastrointestinal mucosal injury are few. We investigated the efficacy of glutamine in rat anticancer drug-induced enteritis model. In this study, we used twenty male SD rats. They were divided into control, 5-fluorouracil (5-FU) (orally administered at 20 mg/kg day), 5-FU+glutamine (1000 mg/kg/day) and 5-FU+glutamine+fiber and oligosaccharide (GFO(®)) (1000 mg/kg/day) groups. All groups were sacrificed on day 6 and upper jejunums were excised. The jejunal villous height was measured in specimens. IgA level in jejunal washing solution, and serum diamine oxidase activity were also measured. The jejunal villous height was recognized as shorter in the specimen from 5-FU treated rats compared with 5-FU+glutamine treated rats (p<0.001). Serum diamine oxidase activity in 5-FU+glutamine group were significantly superior to that in 5-FU group (p=0.028). IgA level in jejunal washing solution tended to be higher in 5-FU+glutamine group than that in 5-FU group (p=0.076). On the other hand, serum diamine oxidase activity and IgA level in jejunal washing solution showed no significant difference between 5-FU+GFO and 5-FU treatment group. Our results suggest that glutamine showed protective effects on mucosal injury of small intestine in rat anticancer drug-induced enteritis model. (キーワード) glutamine / gastrointestinal toxity / anticancer drug / diamine oxidase / IgA (徳島大学機関リポジトリ) ● Metadata: 109521 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.61.59 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24705750 ● CiNii @ 国立情報学研究所 (CRID): 1390001204244693120 ● Summary page in Scopus @ Elsevier: 2-s2.0-84897937797 (徳島大学機関リポジトリ: 109521, DOI: 10.2152/jmi.61.59, PubMed: 24705750, CiNii: 1390001204244693120, Elsevier: Scopus)
26.	Takaaki Shimohata, Kazuaki Mawatari, Hitomi Iba, Masakazu Hamano, Sachie Negoro, Shoko Asada, Mutsumi Aihara, Akiko Hirata, Zehong Su and Akira Takahashi : VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of Vibrio parahaemolyticus., Canadian Journal of Microbiology, Vol.58, No.8, 1002-1007, 2012. (要約) Vibrio parahaemolyticus is a pathogenic Vibrio species that causes food-borne acute gastroenteritis, often related to the consumption of raw or undercooked seafood. Vibrio parahaemolyticus has 2 type III secretion systems (T3SS1 and T3SS2). Here, we demonstrate that VP1657 (VopB1) and VP1656 (VopD1), which share sequence similarity with Pseudomonas genes popB (38%) and popD (36%), respectively, are essential for translocation of T3SS1 effectors into host cells. A VP1680CyaA fusion reporter system was constructed to observe effector translocation. Using this reporter assay we showed that the VopB1 and VopD1 deletion strains were unable to translocate VP1680 to host cell but that the secretion of VP1680 into the culture medium was not affected. VopB1 or VopD1 deletion strains did not enhance cytotoxicity and failed to activate mitogen-activated protein kinases and secretion of interleukin-8, which depend on VP1680. Thus, we conclude that VopB1 and VopD1 are essential components of the translocon. To target VopB1 and VopD1 may have therapeutic potential for the treatment or prevention in V. parahaemolyticus infection. (出版サイトへのリンク) ● Publication site (DOI): 10.1139/w2012-081 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22827847 ● Summary page in Scopus @ Elsevier: 2-s2.0-84864769777 (DOI: 10.1139/w2012-081, PubMed: 22827847, Elsevier: Scopus)
27.	Thanh Tam Thi Le, Kazuaki Mawatari, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, Mutsumi Aihara, Akiko Hirata, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi : VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress., Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1820, No.10, 1686-1692, 2012. (要約) The V. parahaemolyticus FeSOD VP2118 may enhance ROS resistance and could promote its survival in the intestinal tract to facilitate host tissue infection. (出版サイトへのリンク) ● Publication site (DOI): 10.1016/j.bbagen.2012.06.019 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22771831 ● Summary page in Scopus @ Elsevier: 2-s2.0-84863814713 (DOI: 10.1016/j.bbagen.2012.06.019, PubMed: 22771831, Elsevier: Scopus)
28.	Tuyet Le Thi Nhung, Hirofumi Nagata, Akira Takahashi, Mutsumi Aihara, Toshihiro Okamoto, Takaaki Shimohata, Kazuaki Mawatari, Masatake Akutagawa, Yohsuke Kinouchi and Masanobu Haraguchi : Sterilization effect of UV light on Bacillus spores using TiO(2) films depends on wavelength., The Journal of Medical Investigation : JMI, Vol.59, No.1-2, 53-58, 2012. (要約) UV light and photocatalysts such as titanium dioxide (TiO(2)) and silver (Ag) are useful for disinfection of water and surfaces. However, the effect of UV wavelength on photocatalytic disinfection of spores is not well understood. Inactivation of Bacillus spores has been examined using different UV wavelengths and TiO(2) or TiO(2)/Ag composite materials. The level of UVA disinfection of Bacillus anthracis and Bacillus brevis vegetative cells increased with the presence of the TiO(2) and Ag photocatalysts, but had little effect on their spores. B. brevis spores were slightly more sensitive to UVB and UVC than the spores of B. atrophaeus. Photocatalytic sterilization against spores was strongest in UVC and UVB and weakest in UVA. The rate of inactivation of Bacillus spores was significantly increased by the presence of TiO(2), but was not markedly different from that induced by the presence of Ag. Therefore, TiO(2)/Ag plus UVA can be used for the sterilization of vegetative cells, while TiO(2) and UVC are effective against spores. (キーワード) Bacillus anthracis / Silver / Spores, Bacterial / 殺菌 (sterilization) / チタン (titanium) / Ultraviolet Rays (徳島大学機関リポジトリ) ● Metadata: 106002 (出版サイトへのリンク) ● Publication site (DOI): 10.2152/jmi.59.53 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22449993 ● Search Scopus @ Elsevier (PMID): 22449993 ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.59.53 (徳島大学機関リポジトリ: 106002, DOI: 10.2152/jmi.59.53, PubMed: 22449993)
29.	Tuyet Nhung Thi Le, Hirofumi Nagata, Mutsumi Aihara, Akira Takahashi, Toshihiro Okamoto, Takaaki Shimohata, Kazuaki Mawatari, Yhosuke Kinouchi, Masatake Akutagawa and Masanobu Haraguchi : Additional effects of silver nanoparticles on bactericidal efficiency depend on calcination temperature and dip-coating speed., Applied and Environmental Microbiology, Vol.77, No.16, 5629-5634, 2011. (要約) There is an increasing interest in the application of photocatalytic properties for disinfection of surfaces, air, and water. Titanium dioxide is widely used as a photocatalyst, and the addition of silver reportedly enhances its bactericidal action. However, the synergy of silver nanoparticles and TiO(2) is not well understood. The photocatalytic elimination of Bacillus atrophaeus was examined under different calcination temperatures, dip-coating speeds, and ratios of TiO(2), SiO(2), and Ag to identify optimal production conditions for the production of TiO(2)- and/or TiO(2)/Ag-coated glass for surface disinfection. Photocatalytic disinfection of pure TiO(2) or TiO(2) plus Ag nanoparticles was dependent primarily on the calcination temperature. The antibacterial activity of TiO(2) films was optimal with a high dip-coating speed and high calcination temperature (600°C). Maximal bacterial inactivation using TiO(2)/Ag-coated glass was also observed following high-speed dip coating but with a low calcination temperature (250°C). Scanning electron microscopy (SEM) showed that the Ag nanoparticles combined together at a high calcination temperature, leading to decreased antibacterial activity of TiO(2)/Ag films due to a smaller surface area of Ag nanoparticles. The presence of Ag enhanced the photocatalytic inactivation rate of TiO(2), producing a more pronounced effect with increasing levels of catalyst loading. (キーワード) Anti-Bacterial Agents / Bacillus / Catalysis / Disinfectants / Glass / Hot Temperature / Metal Nanoparticles / Microbial Sensitivity Tests / Microscopy, Electron, Scanning / 光化学 (photochemistry) / Silicon Dioxide / Silver / Time Factors / チタン (titanium) / Ultraviolet Rays (出版サイトへのリンク) ● Publication site (DOI): 10.1128/AEM.00049-11 (文献検索サイトへのリンク) ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21724887 ● Summary page in Scopus @ Elsevier: 2-s2.0-80052609659 (DOI: 10.1128/AEM.00049-11, PubMed: 21724887, Elsevier: Scopus)

総説・解説:

1.	白井昭博, 粟飯原睦美 : LEDの放射特性を利用した微生物制御と食品衛生分野への応用, 日本防菌防黴学会誌, Vol.50, No.3, 121-128, 2022年3月. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1521699231205516544 (CiNii: 1521699231205516544)
2.	中橋睦美, 髙橋章 : 紫外線LEDの医療への応用, 小児科, Vol.57, No.8, 1011-1016, 2016年7月.
3.	中橋睦美, 髙橋章 : 紫外線LEDを用いた殺菌システムと応用 (特集先進的環境プロセス技術の展開), ケミカルエンジニヤリング = Chemical engineering, Vol.61, No.6, 428-432, 2016年6月. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1520010380144585472 (CiNii: 1520010380144585472)
4.	髙橋章, 粟飯原睦美, 馬渡一諭, 芥川正武 : 近紫外線発光ダイオードを用いた殺菌システム, 食品分野における非加熱殺菌技術, 150-166, 2013年11月.
5.	粟飯原睦美, 髙橋章 : 近紫外線発光ダイオードを用いた食肉表面殺菌法の開発, 養牛の友, No.445, 76-79, 2013年4月. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1522543654692705408 (CiNii: 1522543654692705408)
6.	常冨愛香里, 粟飯原睦美, 髙橋章 : 新UVA-LEDの環境浄化への応用と展開 (特集紫外線利用による環境浄化の現状と課題), 空気清浄 : コンタミネーションコントロール, Vol.51, No.3, 33-36, 2013年. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1520854805035201792 (CiNii: 1520854805035201792)
7.	粟飯原睦美, 髙橋章 : 食品製造分野におけるLED殺菌技術の可能性 (特集洗浄・殺菌技術の近況), ジャパンフ-ドサイエンス, Vol.48, No.6, 20-26, 2009年6月. (文献検索サイトへのリンク) ● CiNii @ 国立情報学研究所 (CRID): 1522543655595785856 (CiNii: 1522543655595785856)

国際会議:

1.	N. Torigoe, Mutsumi Aihara, Q. Lin, K. Takebayashi, B. Liu, Megumi Nagahara and Takeshige Otoi : Development of porcine embryos cultured in media irradiated with ultraviolet at 228 and 260 nm wavelength., The 50th Conference of the International Embryo Technology Society, Jan. 2024.
2.	Akira Takahashi, Katsuyuki Miyawaki, Kazuaki Mawatari, Takeshi Nikawa, Mutsumi Aihara, Fukushima Shiho, Akizawa Shinta, Yamashita Michiyo and Koi Yumena : Development of closed-circulation soybean cultivation system applicable to extreme environments, The 3rd Japan-France International Symposium on Space Nutrition/Medicine, Kyoto, Nov. 2023.
3.	Nomoto Kazuki, Izumi Takuto, Mutsumi Aihara, Takagi Kousuke, Suzuki Misato, Matsumura Takumi, Akihiro Shirai, Takashi Mukai and Retsuo Kawakami : Damage-Less Microbial Inactivation of Plant Nutrient Solutions Irradiated with Atmospheric-Pressure Low-Temperature Air Plasma Jets, Proceedings of International Symposium of Dry Process 2022, 127-128, Osaka, Nov. 2022.
4.	Toshitaka Ikehara, Mutsumi Nakahashi, Takahiro Emoto, Masatake Akutagawa, Koichiro Tsuchiya, Akira Takahashi and Yohsuke Kinouchi : Effects of reactive oxygen species induced by 405 nm light irradiation on Hela S3 cells, The Joint Annual Meeting of the Bioelectromagnetics Society and the European BioElectromagnetics Association, Montpellier, Jun. 2019.
5.	Junko Kido, Takaaki Shimohata, sachie amano, sho hatayama, yuri sato, yuna kanda, aya tentaku, shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : CFTR reduced microtubule-mediated Campylobacter jejuni invasion, 52th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Feb. 2018.
6.	Tsunedomi Akari, Masamura Akinori, Mutsumi Nakahashi, Nishisaka Risa, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Disinfection system with UVA-LED for hydroponic nutrient solution, 6th FEMS Microbiology Congress, Jun. 2015.
7.	Hatayama Sho, Takaaki Shimohata, Negoro Sachie, Sato Yuri, Kido Junko, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : The denudation of lateral part promote Campylobacter jejuni invasion in Caco-2 cells., 6th FEMS Microbiology Congress, Jun. 2015.
8.	Takashi Uebanso, Takaaki Shimohata, Iba Hitomi, Nishimura Kazuya, Taniguchi Yuichi, Kazuki Horikawa, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi : COMBINATION BETWEEN A FEW T3SS INJECTISOME AND A LOT EFFECTOR FOR KILLING HOST CELLS ON VIBRIO PARAHAEMOLYTICUS, 6th FEMS Microbiology Congress, Jun. 2015.
9.	Takaaki Shimohata, Negoro Sachie, Kido Junko, Hatayama Sho, Sato Yuri, Iba Hitomi, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : INFECTION OF CAMPYLOBACTER JEJUNI REDUCES CFTR MEDIATED CL- SECRETION IN T-84, 6th FEMS Microbiology Congress, Jun. 2015.
10.	Ayumi Yoshimoto, Takashi Uebanso, Kazuaki Mawatari, Mutsumi Nakahashi, Takaaki Shimohata and Akira Takahashi : Low dose antibiotics treatment in the prenatal period affect newborns health in later life, 12th Asian Congress of Nutrition, 70, May 2015.
11.	Takashi Uebanso, Ai Ohnishi, Takaaki Shimohata, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi : Acceptable daily intake levels of sucralose consumption reduces fecal Clostridium cluster 4 in mouse, 12th Asian Congress of Nutrition, 70, May 2015.
12.	Takaaki Shimohata, Sachie Negoro, Sho Hatayama, Yuri Sato, Hitomi Iba, Mutsumi Aihara, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : Campylobacter jejuni Infection Reduces Cftr Mediated Cl- Secretion In T-84, American Society for Microbiology 114th General Meeting, May 2014.
13.	Mutsumi Aihara, Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi : Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus, 48th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Feb. 2014.
14.	Sachie Negoro, Takaaki Shimohata, Syo Hatayama, Mari Matsumoto, Yuri Sato, Mutsumi Aihara, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : Suppression of CFTR-mediated Cl- transport in Campylobacter jejuni infection, International Symposium Hannover - Tokushima Research Communication, Aug. 2013.
15.	Takashi Uebanso, Hitomi Iba, Shoko Asada, Takaaki Shimohata, Mutsumi Aihara, Kazuaki Mawatari and Akira Takahashi : Regulation and effects of VP1671 (VscQ) on virulence of Vibrio parahaemolyticus, 5th FEMS Microbiology Congress, Jul. 2013.
16.	Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi : Synergistic bactericidal effect of ultraviolet light with a combination of different wavelength, 5th FEMS Microbiology Congress, Jul. 2013.
17.	Kazuaki Mawatari, Tam Thanh Thi Le, Miki Maetani, Tomomi Yamamoto, Airi Honjo, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi : VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress, 5th FEMS Microbiology Congress, Jul. 2013.
18.	Kazuaki Mawatari, Tam Thanh Thi Le, Miki Maetani, Tomomi Yamamoto, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi : VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress, US-Japan Cooperative Medical Science Program, 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections, Dec. 2012.
19.	Masachika Ishizaki, Yusuke Manabe, Mutsumi Aihara, Akira Takahashi, Masatake Akutagawa, Takahiro Emoto, Yohsuke Kinouchi and Toshitaka Ikehara : Improvement of a pipe type UVA-LED sterilizer using a condenser lens, 34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract), 189-190, Brisbane, Jun. 2012. (要約) UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた．
20.	Toshitaka Ikehara, Mutsumi Aihara, Zehong Su, Akira Takahashi, Masatake Akutagawa and Yohsuke Kinouchi : Effects of UVA radiation on growth of RAW 264.7 cells, 34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract), 179-180, Brisbane, Jun. 2012. (要約) マウス由来のRAW 264.7細胞に対してUVAを照射した際の増殖の違いについて検討した．
21.	Kazuaki Mawatari, Yuka Isumi, Minami Hirayama, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, Mutsumi Aihara, Takaaki Shimohata and Akira Takahashi : OtnA-OtnB modulate capsular polysaccharide and polymyxin B resistance in Vibrio parahaemolyticus, 112th General Meeting of the American Society for Microbiology, Jun. 2012.
22.	Mutsumi Aihara, Kazuaki Mawatari, Takaaki Shimohata and Akira Takahashi : Bactericidal Effect of Ultraviolet Light with a Combination of Different Wavelengths, 112th General Meeting of the American Society for Microbiology, Jun. 2012.
23.	Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi : VPA1604, a protein containing phosphorylated tyrosine, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus, 4th FEMS Microbiology Congress, Jun. 2011.
24.	Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi : VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus, US-Japan Cooperative Medical Science Program, 45th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel, Kyoto, Dec. 2010.

国内講演発表:

1.	砂川佳吾, Md Hanif Ali, 山西百音, 小林美佑, 公門瑞希, Rumana Yesmin Hasi, 粟飯原睦美, 田中保 : ペルオキシソーム欠損細胞における極長鎖脂肪酸毒性とオレイン酸による毒性解除, 日本農芸化学会中四国支部第67回講演会, 2024年1月.
2.	松本尚子, 髙井誠道, 藤田智帆, MD MAJIDUL ISLAM, Rumana Yesmin Hasi, 粟飯原睦美, 田中保 : 植物におけるグリコシルイノシトールホスホセラミドとその分解酵素の解析, 日本農芸化学会中四国支部第67回講演会, 2024年1月.
3.	小野実優, 石田快, 牧本真奈, 下畑隆明, 上番増喬, 粟飯原睦美, 芥川正武, 榎本崇宏, 馬渡一諭, 岩田剛敏, 髙橋章 : UVA 照射による Campylobacter jejuni の上皮定着性に対する影響, 第16 回日本カンピロバクター研究会総会, 2023年12月.
4.	髙井誠道, Rumana Yesmin Hasi, 松本尚子, 藤田智帆, MD MAJIDUL ISLAM, 粟飯原睦美, 石川寿樹, 今井博之, 田中保 : TLCイメージングを用いた植物スフィンゴ脂質の分解経路の解析, 第96回日本生化学大会プログラム集p126, 2023年10月.
5.	Ali Hanif Md, Kobayashi Miyu, Kumon Mizuki, Yamanishi Mone, Hasi Yesmin Rumana, Mutsumi Aihara and Tamotsu Tanaka : Effect of very long-chain fatty acids on viability of different cells, 第96回日本生化学大会プログラム集p127, Oct. 2023.
6.	山西百音, Md Hanif Ali, 小林美佑, 公門瑞希, 粟飯原睦美, 田中保 : 可溶化した極長鎖脂肪酸の細胞への取り込みと毒性の解析, 第96回日本生化学大会プログラム集 p127, 2023年10月.
7.	相澤心太, 小井優萌那, 山下路代, 白石志帆, 宮脇克行, 粟飯原睦美, 二川健, 上番増喬, 馬渡一諭, 髙橋章 : 極地環境での大豆栽培方法の確立, 第56回日本栄養·食糧学会中国·四国支部大会, 2023年10月.
8.	Rumana Yesmin Hasi, 倭村直宏, 砂川佳吾, 髙井誠道, 松本尚子, 藤田智帆, Md Hanif Ali, MD MAJIDUL ISLAM, 石川寿樹, 梅村ゆうた, 田中秀則, 今井博之, 粟飯原睦美, 田中保 : Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide, 第65回日本脂質生化学会, Vol.65, 237-240, 2023年6月.
9.	Md Hanif Ali, 小林美佑, 公門瑞希, 山西百音, 粟飯原睦美, 田中保 : 極長鎖脂肪酸の可溶化と細胞への取り込み解析, 第64回日本生化学中国・四国支部例会プログラム・講演要旨集 p33, 2023年5月.
10.	粟飯原睦美, 泉匠人, 白井昭博, 向井孝志, 川上烈生 : 非平衡大気圧プラズマジェットを用いた植物栽培における養液の衛生管理技術の開発, 第96回日本細菌学会総会, 2023年3月. (キーワード) プラズマジェット / 植物栽培養液 / 衛生管理技術 / ダメージレス
11.	石田快, 粟飯原睦美, 下畑隆明, 北山栞里, 勢川玲花, 上番増喬, 馬渡一諭, 鈴木浩司, 芥川正武, 榎本崇宏, 山本光生, 富久章子, 和田敬宏, 岡本雅之, 伊藤浩, 安野卓, 木内陽介, 髙橋章 : 鶏舎におけるUV-LED導入による衛生環境改善効果の検討, 第43回日本食品微生物学会学術総会, 2022年9月.
12.	石田快, 粟飯原睦美, 下畑隆明, 北山栞里, 勢川玲花, 上番増喬, 馬渡一諭, 鈴木浩司, 芥川正武, 榎本崇宏, 山本光生, 富久章子, 和田敬宏, 岡本雅之, 伊藤浩, 安野卓, 木内陽介, 髙橋章 : 鶏舎内へのUV―LED導入による鶏の生育及び衛生環境の改善効果の検討, 日本家禽学会2022年度秋季大会, 2022年9月.
13.	田中保, Md Hanif Ali, 小林美佑, Rumana Yesmin Hasi, 粟飯原睦美, 林順司, 川上竜巳 : 極長鎖脂肪酸による毒性とその解毒装置としてのペルオキシソームの役割, 脂質栄養学, Vol.31, No.2, 143, 2022年9月.
14.	Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka : Distribution and characterization of glycosylinositol phosphoceramide specific phospholipase D in Brassica plants, 第64回日本脂質生化学会, Vol.64, 272-275, Jun. 2022.
15.	Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka : Peroxisomes attenuate lipotoxicity of very-long-chain fatty acids, 第64回日本脂質生化学会, Vol.64, 43-46, Jun. 2022.
16.	Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka : Metabolism and biological effect of very-long-chain fatty acid in peroxisome-deficient cells, 第63回日本生化学中国・四国支部例会, May 2022.
17.	北山栞里, 下畑隆明, 白石志帆, 石田快, 緒方美裕起, 上番増喬, 馬渡一諭, 粟飯原睦美, 芥川正武, 榎本崇宏, 鈴木浩司, 安野卓, 伊藤浩, 和田敬宏, 岡本雅之, 富久章子, 髙橋章, 木内陽介 : UV-LEDによる鶏舎内光環境の構築, LED総合フォーラム2022 in 徳島, 169-172, 2022年1月.
18.	Toshitaka Ikehara, Mutsumi Aihara, Koichiro Tsuchiya, Takahiro Emoto, Masatake Akutagawa, Akira Takahashi and Yohsuke Kinouchi : Studies of reactive oxygen species scavenging system of cultured cells by using LED light irradiation, LED総合フォーラム2022 in 徳島, 193-198, Jan. 2022.
19.	Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka : Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells, 第94回日本生化学大会, Nov. 2021.
20.	Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka : Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants, 第94回日本生化学大会, Nov. 2021.
21.	Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka : Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells, 第62回日本生化学中国・四国支部例会, Sep. 2021.
22.	Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka : Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants, 第62回日本生化学中国・四国支部例会, Sep. 2021.
23.	高尾祐希, 川上烈生, 白井昭博, 味元勇樹, 粟飯原睦美, 向井孝志 : アナターゼ型光触媒TiO2ナノ粒子による非接触殺菌効果, 日本防菌防黴学会第48回年次大会, 136, 2021年9月.
24.	泉匠人, 川上烈生, 粟飯原睦美, 松村拓海, 白井昭博, 宮脇克行, 向井孝志 : 植物養液への大気圧低温空気プラズマジェット殺菌効果, 2021年度応用物理・物理系学会中国四国支部合同学術講演会, 40, 2021年7月.
25.	Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya and Tamotsu Tanaka : Uptake and metabolism of very-long-chain fatty acid in animal cells, 第63回日本脂質生化学会, Jun. 2021.
26.	泉匠人, 川上烈生, 粟飯原睦美, 白井昭博, 宮脇克行, 向井孝志 : 大気圧低温プラズマジェット照射による植物養液殺菌効果, 第68回春季応用物理学会学術講演会, 07-070, 2021年3月.
27.	Rumana Yesmin Hasi, Yoshimichi Takai, Hanif Ali, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Kaori Kanemaru and Tamotsu Tanaka : Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate from cabbage leaves and their chemical stabilities., 日本農芸化学会2020年度中四国支部大会(第57回講演会), Sep. 2020.
28.	泉匠人, 川上烈生, 粟飯原睦美, 白井昭博, 宮脇克行, 向井孝志 : 植物養液への非平衡大気圧空気プラズマジェット照射効果, 2020年度応用物理・物理系学会中国四国支部合同学術講演会, 10, 2020年8月.
29.	高見直樹, 川上烈生, 中橋睦美, 新部正人, 中野由崇, 東知里, 向井孝志 : 空気プラズマジェット照射による種子の発芽促進効果, 令和元年度電気関係学会四国支部連合大会講演論文集, 43, 2019年9月.
30.	畑山翔, 下畑隆明, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 中本晶子, 首藤恵泉, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni汚染調理器具に対するUVA-LED照射殺菌の有効性について, 第39回日本食品微生物学会学術総会, 2018年9月.
31.	木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTR発現はCampylobacter jejuniの微小管を介した輸送を抑制する, 2017年度生命科学系学会合同年次大会, 2017年12月.
32.	木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 佐藤優里, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTR reduced microtubule-mediated Campylobacter jejuni invasion, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2017年8月.
33.	上番増喬, 大西愛, 北山礼子, 吉本亜由美, 中橋睦美, 下畑隆明, 馬渡一諭, 髙橋章 : 許容上限量の甘味料の摂取が腸内細菌叢と宿主へ及ぼす影響の解析, 日本栄養食糧学会, 243, 2017年5月.
34.	上番増喬, 吉本亜由美, 下畑隆明, 中橋睦美, 馬渡一諭, 髙橋章 : 食事成分の腸内細菌叢への影響を代謝産物から読み解けるか?, 日本細菌学会総会, 42, 2017年3月.
35.	渡邊瞳, 馬渡一諭, 西坂理沙, 中橋睦美, 常冨愛香里, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : 透析液の細菌汚染調査と近視外LEDによる殺菌効果の評価, 第69回日本細菌学会中国・四国支部総会, 2016年10月.
36.	下畑隆明, 福島志帆, 佐藤優里, 扶川留音, 木戸純子, 神田結奈, 天宅あや, 畑山翔, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniの宿主細胞内生存戦力に関する検討, 第69回日本細菌学会中国・四国支部総会, 2016年10月.
37.	木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTR発現によるCampylobacter jejuniの侵入抑制機序の解明, 第69回日本細菌学会中国・四国支部総会, 2016年10月.
38.	中橋睦美, 常冨愛香里, 上番増喬, 下畑隆明, 馬渡一諭, 芥川正武, 木内陽介, 髙橋章 : 紫外線LEDと次亜塩素酸ナトリウムの併用による食品殺菌装置の開発, 第37回日本食品微生物学会学術総会, 2016年9月.
39.	髙橋章, 下畑隆明, 馬渡一諭, 上番増喬, 常冨愛香里, 中橋睦美, 芥川正武, 木内陽介 : 飲料水の色が近紫外線殺菌にあたえる影響, 第37回日本食品微生物学会学術総会, 2016年9月.
40.	常冨愛香里, 下畑隆明, 永田早紀恵, 天野幸恵, 中橋睦美, 原田優美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有用性について, 第37回日本食品微生物学会学術総会, 2016年9月.
41.	下畑隆明, 福島志帆, 佐藤優里, 扶川留音, 木戸純子, 神田結奈, 天宅あや, 畑山翔, 中橋睦美, 上番増喬, 原田永勝, 馬渡一諭, 髙橋章 : 上皮細胞に侵入したCampylobacter jejuniのエネルギー獲得機構について, 第37回日本食品微生物学会学術総会, 2016年9月.
42.	西坂理沙, 渡邊瞳, 馬渡一諭, 中橋睦美, 常冨愛香里, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : 県内医療施設の透析液細菌汚染調査と近紫外LEDによる殺菌効果の評価, 第253回徳島医学会学術集会, 2016年7月.
43.	木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTR発現によりCampylobacter jejuniの微小管依存性侵入機構が抑制される, 第253回徳島医学会学術集会, 2016年7月.
44.	吉本亜由美, 上番増喬, 下畑隆明, 中橋睦美, 馬渡一諭, 髙橋章 : 妊娠期の母親の低用量の抗菌薬摂取が子供の健康に及ぼす影響の解析, 第253回徳島医学会学術集会, 24, 2016年7月.
45.	畑山翔, 下畑隆明, 吉兼道子, 天野幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : 食中毒原因菌Campylobacter jejuniは腸管上皮細胞側面の露出により宿主内へ効率的に侵入する, 第70回日本栄養・食糧学会大会, 2016年5月.
46.	常冨愛香里, 下畑隆明, 後藤茉凜, 天野幸恵, 中橋睦美, 原田優美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : UVA-LED殺菌システムによるCampylobacter jejuni食中毒の予防, 第70回日本栄養・食糧学会大会, 2016年5月.
47.	木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniの細胞侵入は細胞膜表面CFTRにより調節される, 第89回日本細菌学会総会, 2016年3月.
48.	下畑隆明, 佐藤優里, 扶川留音, 木戸純子, 天野幸恵, 畑山翔, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : 代謝解析で明らかとなったCampylobacter jejuni感染による宿主細胞での特徴的なアミノ酸変動について, 第89回日本細菌学会総会, 2016年3月.
49.	吉本亜由美, 上番増喬, 中橋睦美, 下畑隆明, 馬渡一諭, 髙橋章 : Effect of low dose antibiotics administration during prenatal period on newborns postnatal health, 第89回日本細菌学会総会, 2016年3月.
50.	常冨愛香里, 正村彰規, 中橋睦美, 西坂理沙, 後藤茉凛, 馬渡一諭, 下畑隆明, 上番増喬, 宮脇克行, 芥川正武, 木内陽介, 髙橋章 : 植物工場の養液殺菌システムの開発, 第251回徳島医学会学術集会, 2015年8月.
51.	畑山翔, 下畑隆明, 吉兼道子, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniの上皮細胞への侵入は細胞のラテラル面の露出により増加する, 第251回徳島医学会学術集会, 2015年8月.
52.	下畑隆明, 木戸純子, 根来幸恵, 畑山翔, 佐藤優里, 射場仁美, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTR regulates Campylobacter jejuni invasion in cultured cells, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2015年7月.
53.	馬渡一諭, 本庄アイリ, 安井実希, 畑山翔, 中橋睦美, 下畑隆明, 髙橋章 : 腸炎ビブリオのVP2357遺伝子変異は近紫外線(UVA)照射耐性獲得に寄与する, 第88回日本細菌学会総会, 2015年3月.
54.	Quang Ngoc Phan, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Nakahashi and Akira Takahashi : DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus, 第88回日本細菌学会総会, Mar. 2015.
55.	畑山翔, 下畑隆明, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniは腸管上皮の側部より効率的に侵入する, 第88回日本細菌学会総会, 2015年3月.
56.	Phan Quang Ngoc, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Nakahashi and Akira Takahashi : DNA-binding protein HU coordinate pathogenicity in Vibrio parahaemolyticus, 第67回日本細菌学会中国・四国支部総会, Oct. 2014.
57.	常冨愛香里, 中橋睦美, 西坂理沙, 馬渡一諭, 下畑隆明, 上番増喬, 宮脇克行, 芥川正武, 木内陽介, 髙橋章 : 水耕栽培用養液の還流型殺菌システムの開発, 第67回日本細菌学会中国・四国支部総会, 2014年10月.
58.	木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniによる細胞侵入とCFTRの関連について, 第67回日本細菌学会中国・四国支部総会, 2014年10月.
59.	星山哲平, 芥川正武, 榎本崇宏, 中橋睦美, 林田麻里王, 髙橋章, 馬渡一諭, 池原敏孝, 小中信典, 木内陽介 : UV及び可視光照射に対する腸炎ビブリオの遺伝子発現解析, 電気関係学会四国支部連合大会講演論文集, No.14-1, 229, 2014年9月. (要約) 腸炎ビブリオを紫外線および可視光に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．その結果，UV-A領域の光を照射した際，酸化修復に関する遺伝子の働きが抑えられること，緑色の光を照射した際に鞭毛関連の遺伝子が活性化することなどが示された．
60.	本庄アイリ, 馬渡一諭, 前谷実希, 岩本夏実, 山下智子, 中橋睦美, 下畑隆明, 上番増喬, 髙橋章 : Anti-sigma factor VP2357の遺伝子変異はVibrio parahaemolyticusの近紫外線(UVA)耐性獲得に関与する, 第249回徳島医学会学術集会, 2014年7月.
61.	Phan Quang Ngoc, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Aihara and Akira Takahashi : DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus., 第249回徳島医学会学術集会, Jul. 2014.
62.	畑山翔, 下畑隆明, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni 感染による腸管上皮細胞のイオン輸送に関する検討, 第249回徳島医学会学術集会, 2014年7月.
63.	星山哲平, 芥川正武, 榎本崇宏, 中橋睦美, 林田麻里王, 髙橋章, 馬渡一諭, 池原敏孝, 小中信典, 木内陽介 : 異なる波長の光照射に対する腸炎ビブリオの遺伝子発現解析, 電子情報通信学会技術研究報告(MEとバイオサイバネティックス), Vol.114, No.154, 19-24, 2014年7月. (要約) 本研究は細菌へどのような光を照射すると，どのような機能が活性されるかについて考察するものである．現在，光照射が細菌にもたらす影響が多数報告されており，例えば紫外(UV)照射を行う事で殺菌効果が得られ，可視光照射では光受容体という機能の存在が示されている．本研究では，このような異なる波長の光照射に対する細菌への影響を，遺伝子発現解析により考察した．その結果， UV及び可視光の各波長の光照射時について変動の見られた腸炎ビブリオ遺伝子を特定する事ができた．また， 520nmの光を腸炎ビブリオに照射すると，腸炎ビブリオの鞭毛遊走機能が活性化される可能性が示唆された． (キーワード) 光照射 / 波長依存性 / 遺伝子発現解析 / 腸炎ビブリオ
64.	常冨愛香里, 正村彰規, 粟飯原睦美, 馬渡一諭, 下畑隆明, 上番増喬, 石崎仁愛, 芥川正武, 木内陽介, 髙橋章 : 近紫外線による水耕栽培用培養液の殺菌法の開発, 第68回日本栄養・食糧学会大会, 2014年5月.
65.	本庄アイリ, 馬渡一諭, 前谷実希, 常冨愛香里, 畑山翔, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 : Vibrio parahaemolyticusのkatGgene, VPA0768発現上昇は近紫外線(UVA)耐性獲得に関与する, 第68回日本栄養・食糧学会大会, 2014年5月.
66.	根耒幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni 感染はCFTR機能を抑制する, 第68回日本栄養・食糧学会大会, 2014年5月.
67.	馬渡一諭, 本庄アイリ, 常冨愛香里, 畑山翔, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 : 近紫外線(UVA)照射により酸化される腸炎ビブリオタンパク質の同定, 第87回日本細菌学会総会, 2014年3月.
68.	下畑隆明, 根耒幸恵, 畑山翔, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni感染はT84細胞におけるCl-分泌を抑制する, 第87回日本細菌学会総会, 2014年3月.
69.	林田麻里王, 粟飯原睦美, 上番増喬, 下畑隆明, 馬渡一諭, 髙橋章, 星山哲平, 芥川正武, 榎本崇宏, 木内陽介 : 紫外線・可視光照射により変動する微生物遺伝子の網羅的解析, 第66回日本細菌学会中国・四国支部総会, 2013年10月.
70.	本庄アイリ, 枝川美幸, 馬渡一諭, 前谷実希, 粟飯原睦美, 下畑隆明, 上番増喬, 上手麻希, 間世田英明, 髙橋章 : トランスポゾン挿入変異ライブラリを用いた腸炎ビブリオの近紫外線関連遺伝子の探索, 第66回日本細菌学会中国・四国支部総会, 2013年10月.
71.	星山哲平, 芥川正武, 榎本崇宏, 小中信典, 木内陽介, 粟飯原睦美, 林田麻里王, 髙橋章 : UV照射に対する腸炎ビブリオの遺伝子発現解析, 平成25年度電気関係学会四国支部連合大会講演論文集, 252, 2013年9月. (要約) 腸炎ビブリオを紫外線に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．UV-A，UV-B，UV-Cそれぞれの代表的な波長について，腸炎ビブリオにUV照射した後に解析を行い，各波長ごとの遺伝子発現の違いについて調査した．
72.	Mutsumi Aihara, Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi : Synergistic bactercidal effect of Ultraviolet light with a combination of different, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Aug. 2013.
73.	林田麻里王, 粟飯原睦美, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 星山哲平, 榎本崇宏, 芥川正武, 木内陽介 : DNA マイクロアレイを用いた微生物遺伝子の網羅的解析, 第247回徳島医学会学術集会, 2013年8月.
74.	常冨愛香里, 粟飯原睦美, 石崎仁愛, 上番増喬, 下畑隆明, 芥川正武, 馬渡一諭, 髙橋章, 正村彰規 : UVA-LED を用いた水耕栽培用養液の殺菌, 第247回徳島医学会学術集会, 2013年8月.
75.	本庄アイリ, 馬渡一諭, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 : VP0221とVPA1604は腸炎ビブリオの莢膜多糖類を調節し，抗菌ペプチドと補体耐性に働く, 第247回徳島医学会学術集会, 2013年8月.
76.	根来幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni 感染によるCl‐輸送チャネルの活性変化, 第247回徳島医学会学術集会, 2013年8月.
77.	畑山翔, 下畑隆明, 根来幸恵, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : 食中毒起因菌カンピロバクターによる下痢の機序に関する研究, 第247回徳島医学会学術集会, 2013年8月.
78.	下畑隆明, 根来幸恵, 畑山翔, 松本麻里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni感染によるクロライド分泌の変化, 第86回日本細菌学会総会, 2013年3月.
79.	馬渡一諭, 黒川香菜, 本庄アイリ, 藤井麻未, 山本智実, 前谷実希, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 : Vibrio parahaemolyticusを用いたUVA紫外線照射による殺菌機構の解析, 第65回日本細菌学会・中国・四国支部総会, 2012年10月.
80.	Tam Thanh Thi Le, Kazuaki Mawatari, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi : VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Aug. 2012.
81.	馬渡一諭, 井角友香, 平山みなみ, 前谷実希, 山本智実, 林田沙也加, 射場仁美, 粟飯原睦美, 下畑隆明, 髙橋章 : Vibrio parahaemolyticus capsular polysaccharide is modulated by two pathways, VP0220-1 and VPA1602-4, 第85回日本細菌学会総会, 2012年3月.
82.	下畑隆明, 射場仁美, 根来幸恵, 浅田翔子, 粟飯原睦美, 馬渡一諭, 髙橋章 : VP1680 induces IL-8 secretion in Vibrio parahaemolyticus infection, 第85回日本細菌学会総会, 2012年3月.
83.	下畑隆明, 射場仁美, 根来幸恵, 浅田翔子, 粟飯原睦美, 馬渡一諭, 髙橋章 : 腸炎ビブリオT3SS1におけるトランスロコンの解析, 第64回日本細菌学会中国・四国支部総会, 2011年10月.
84.	林田沙也加, 粟飯原睦美, 下畑隆明, 馬渡一諭, 芥川正武, 木ノ内陽介, 髙橋章 : 水中の細菌に対するUVA-LEDと塩素との併用殺菌効果, 第64回日本細菌学会中国・四国支部総会, 2011年10月.
85.	馬渡一諭, Yuka Isumi, Minami Hirayama, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, 粟飯原睦美, 下畑隆明, 髙橋章 : VP0220-0221 and VPA1602-1604 modulate capsular polysaccharides in Vibrio parahaemolyticus, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, 2011年8月.
86.	馬渡一諭, 米田由美, 本田真奈美, 射場仁美, 粟飯原睦美, 下畑隆明, 蘇澤紅, 大和正幸, 髙橋章 : Vibrio parahaemolyticus のチロシンキナーゼVPA1604 は高分子量型莢膜多糖類(HMW-CPS)形成を調節する, 第63回日本細菌学会・中国・四国支部総会, 2010年10月.
87.	粟飯原睦美, 大和正幸, 馬渡一諭, 髙橋章, 芥川正武, 木内陽介 : 異なる紫外線波長の併用による殺菌効果, 第63回日本細菌学会・中国・四国支部総会, 2010年10月.
88.	Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi : VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus, 日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Jul. 2010.
89.	前田未来, 粟飯原睦美, 廉馨, 木内陽介, 芥川正武, 髙橋章 : UV-LED を用いた野菜の表面殺菌, 電気関係学会四国支部連合大会講演論文集, 236, 2009年9月.
90.	Mosrafa Gadelmoula, Xin Lian, 前田未来, Mutsumi Aihara, 八木教行, 片山麻衣, 濱本晶子, Masayuki Yamato, Masatake Akutagawa, Yohsuke Kinouchi, Yutaka Nakaya and Akira Takahashi : UVA-LEDを用いた空気殺菌の試み, 第23回生体・生理工学シンポジウム, Sep. 2008.
91.	廉馨, 前田未来, Mutsumi Aihara, Akira Takahashi, 八木教行, 橘聡子, 濱本晶子, Masayuki Yamato, Masatake Akutagawa, 中野政之, Yutaka Nakaya and Yohsuke Kinouchi : UVA-LED装置を用いた野菜の表面殺菌, 第23回生体・生理工学シンポジウム, Sep. 2008.
92.	粟飯原睦美, 廉馨, 前田未来, 橘聡子, 大和正幸, 中野政之, 芥川正武, 木内陽介, 髙橋章, 中屋豊 : 高出力紫外線装置を用いた野菜表面の殺菌, 日本防菌防黴学会第35回年次大会, 2008年8月.

その他・研究会:

1.	高木皓介, 鈴木美里, 松本拓海, 粟飯原睦美, 川上烈生 : 高圧アニーリングにより形成させたグラファイト状窒化炭素のLED光分解効果, 次世代光フォーラム2023 in 徳島, 113-114, 2023年2月.
2.	高尾祐希, 白井昭博, 味元勇樹, 粟飯原睦美, 川上烈生 : LED照射下でのアナターゼTiO2ナノ粒子の非接触殺菌効果, LED総合フォーラム2022 in 徳島, 137-138, 2022年1月.
3.	池原敏孝, 中橋睦美, 土屋浩一郎, 榎本崇宏, 芥川正武, 髙橋章, 木内陽介 : 405 nm LED光照射に誘導される活性酸素が培養HeLaS3細胞に及ぼす影響, LED総合フォーラム2020 in 徳島, 145-148, 2020年2月.
4.	畑山翔, 下畑隆明, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 中本晶子, 首藤恵泉, 上番増喬, 馬渡一諭, 髙橋章 : UVA-LED照射による調理器具汚染Campylobacter jejuniの殺菌効果について, 第11回日本カンピロバクター研究会総会, 2018年12月.
5.	常冨愛香里, 畑山翔, 下畑隆明, 木戸純子, 天野幸恵, 中橋睦美, 上番増喬, 馬渡一諭, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : Campylobacter jejuni汚染対策に向けたUVA-LEDの有用性について, 第10回日本カンピロバクター研究会総会, 2017年12月.
6.	馬渡一諭, 渡邊瞳, 西坂理沙, 中橋睦美, 常冨愛香里, 児島瑞基, 野上夏希, 牧野美鈴, 増田瑠見子, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : 透析液の細菌汚染調査と近紫外発光ダイオードによる殺菌効果の評価, 電子情報通信学会技術研究報告(MEとバイオサイバネティックス), Vol.117, No.165, 13-16, 2017年7月. (キーワード) 人工透析 / 細菌汚染 / 近紫外線 / 発光ダイオード (LED) / 殺菌
7.	畑山翔, 下畑隆明, 天野幸恵, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniの宿主腸管上皮細胞への侵入とTight Junctions形成の関連について, 第9回日本カンピロバクター研究会総会, 2016年11月.
8.	常冨愛香里, 下畑隆明, 後藤茉凜, 天野幸恵, 中橋睦美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 : Campylobacter jejuniに対するUVA-LEDの有効性, 第8回日本カンピロバクター研究会総会, 2015年12月.
9.	下畑隆明, 木戸純子, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 : CFTRはCampylobacter jejuniの侵入抑制に寄与する, 第7回日本カンピロバクター研究会総会, 2014年12月.
10.	佐藤優里, 下畑隆明, 根来幸恵, 畑山翔, 木戸純子, 中橋睦美, 上番増喬, 原田永勝, 馬渡一諭, 髙橋章 : Campylobacter jejuni感染とAutophagyの関連について, 第7回日本カンピロバクター研究会総会, 2014年12月.
11.	根来幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuni感染はCFTR機能を抑制する, 第6回日本カンピロバクター研究会, 2013年7月.
12.	畑山翔, 下畑隆明, 根来幸恵, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 : Campylobacter jejuniによる下痢誘発機構の検討, 第6回日本カンピロバクター研究会, 2013年7月.
13.	粟飯原睦美, 新谷佳子, 廉馨, Mostafa Gadelmoula, 大和正幸, 馬渡一諭, 芥川正武, 木内陽介, 髙橋章 : UVA-LED装置を用いた水殺菌装置の開発, LED総合フォーラム論文集, 79-80, 2010年4月.

特許:

1.	三好仁志, 髙橋章, 馬渡一諭, 福島志帆, 粟飯原睦美, 宮脇克行, 二川健, 牧野美鈴 : (2023年3月), 特許第2023-046795号.

科学研究費補助金 (KAKEN Grants Database @ NII.ac.jp)

LED紫外光と殺菌剤の併用によるバイオフィルム形成の抑制を通じた衛生管理法の開発 (研究課題/領域番号: 23K13906 )
カンピロバクターの鶏肉表面汚染低減を目指した、LED光殺菌システムの開発 (研究課題/領域番号: 20K11647 )
LED光照射の傷害修復制御機能を利用したVBNC・損傷菌による食中毒予防法の開発 (研究課題/領域番号: 26870411 )
光照射によるカンピロバクター侵入性制御を目指した新規鶏肉保存システムの開発 (研究課題/領域番号: 26560046 )
光による食生活環境制御法の確立 (研究課題/領域番号: 23650479 )
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講師 : 粟飯原 睦美

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講師 : 粟飯原睦美